Echs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Echs1
Entrez Gene ID	93747
Full Name	enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms	C80529
General protein information	Preferred Names enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Names enoyl-CoA hydratase, mitochondrial SCEH enoyl-CoA hydratase 1 short-chain enoyl-CoA hydratase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F4

mRNA and Protein(s)

mRNA Protein Name

NM_053119.3 NP_444349.1 enoyl-CoA hydratase, mitochondrial precursor

NM_053119.2 NP_444349.1 enoyl-CoA hydratase, mitochondrial precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_053119.3	NP_444349.1	enoyl-CoA hydratase, mitochondrial precursor
NM_053119.2	NP_444349.1	enoyl-CoA hydratase, mitochondrial precursor

Homologies

Rattus norvegicus (Norway rat)	Echs1	NP_511178.1
Homo sapiens (human)	ECHS1	NP_004083.3
Rattus norvegicus (Norway rat)	LOC100911186	XP_003748934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011833	XP_320683.2
Caenorhabditis elegans (roundworm)	ech-6	NP_499156.1
Xenopus tropicalis (tropical clawed frog)	echs1	NP_001005437.1
Canis lupus familiaris (dog)	ECHS1	XP_537945.4
Danio rerio (zebrafish)	echs1	NP_001004529.1
Drosophila melanogaster (fruit fly)	CG6543	NP_610910.1
Neurospora crassa	NCU06448	XP_957339.1
Gallus gallus (chicken)	ECHS1	NP_001264324.1
Pan troglodytes (chimpanzee)	ECHS1	XP_003312886.1
Bos taurus (cattle)	ECHS1	NP_001020377.2
Mus musculus (house mouse)	Echs1	NP_444349.1
Magnaporthe oryzae (rice blast fungus)	MGG_12868	XP_003717318.1

Gene Ontology
Bibliography

Related articles in PubMed

Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase ? and Echs1.
Zhu SG, Xi L, Kukreja RC
Journal of cellular and molecular medicine16(4)950-8(2012 Apr)

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y
Molecular cell23(4)607-18(2006 Aug)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

potential protein targets for the loss of PostC may include F(1)-ATPase gamma, Echs1 and Hsp20 that could regulate cellular ATP consumption/production and defense response to ischaemic stress
Title: Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase ? and Echs1.

The following Echs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Echs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14909	NM_053119.3 Latest version!	Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu14909	NM_053119.2	Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Echs1 ( NM_053119.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444349.1 Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.

Price & Availability↑

CloneID	OMu14909
Clone ID Related Accession (Same CDS sequence)	NM_053119.3 , NM_053119.2
Accession Version	NM_053119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Mus musculus(house mouse)
Product	enoyl-CoA hydratase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK040391.1 and BC072658.1. On Jun 9, 2018 this sequence version replaced NM_053119.2. ##Evidence-Data-START## Transcript exon combination :: BC072658.1, AK040391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCCC TGCGTGCTCT GCTGCCCAGA GCCTGTAGCT CACTGTTGTC CTCAGTCCGC 
TGCCCAGAAC TACGGCGCTT CGCCTCGGGT GCTAACTTTC AGTACATCAT CACAGAAAAG 
AAAGGAAAGA ATAGCAGCGT GGGGCTGATC CAGTTGAACC GCCCCAAAGC ACTCAATGCA 
CTTTGCAATG GCCTGATTGA GGAGCTCAAC CAAGCACTGG AGACCTTTGA GCAAGATCCT 
GCTGTGGGTG CCATTGTGCT CACTGGTGGG GATAAGGCCT TTGCAGCTGG AGCTGACATC 
AAGGAAATGC AGAACCGAAC ATTTCAGGAC TGTTACTCCA GCAAGTTCCT GAGCCACTGG 
GACCACATCA CCCGGGTCAA GAAACCGGTC ATCGCAGCTG TCAATGGTTA TGCTCTTGGT 
GGGGGTTGTG AACTTGCCAT GATGTGTGAT ATCATCTATG CTGGCGAGAA AGCCCAGTTC 
GGACAGCCAG AAATCCTCCT GGGGACCATC CCAGGTGCTG GAGGCACTCA GAGACTCACC 
CGAGCAGTCG GCAAATCGCT AGCAATGGAG ATGGTCCTCA CTGGTGACCG CATCTCAGCT 
CAGGATGCAA AGCAGGCAGG TCTTGTAAGC AAGATTTTTC CTGTTGAAAA ACTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAATTCTA AAATCGTAGT AGCCATGGCG 
AAAGAATCTG TGAATGCAGC CTTTGAGATG ACGTTAACAG AAGGAAATAA GCTGGAGAAG 
AGGCTTTTCT ATTCCACCTT TGCCACCGAT GACCGGAGAG AAGGGATGAC TGCATTTGTA 
GAGAAAAGGA AGGCCAACTT CAAAGACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444349.1
CDS	28..900
Translation	MAALRALLPRACSSLLSSVRCPELRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFVEKRKANFKDH

Target ORF information:

RefSeq Version	NM_053119.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053119.3

ORF Insert Sequence:

ATGGCGGCCC TGCGTGCTCT GCTGCCCAGA GCCTGTAGCT CACTGTTGTC CTCAGTCCGC 
TGCCCAGAAC TACGGCGCTT CGCCTCGGGT GCTAACTTTC AGTACATCAT CACAGAAAAG 
AAAGGAAAGA ATAGCAGCGT GGGGCTGATC CAGTTGAACC GCCCCAAAGC ACTCAATGCA 
CTTTGCAATG GCCTGATTGA GGAGCTCAAC CAAGCACTGG AGACCTTTGA GCAAGATCCT 
GCTGTGGGTG CCATTGTGCT CACTGGTGGG GATAAGGCCT TTGCAGCTGG AGCTGACATC 
AAGGAAATGC AGAACCGAAC ATTTCAGGAC TGTTACTCCA GCAAGTTCCT GAGCCACTGG 
GACCACATCA CCCGGGTCAA GAAACCGGTC ATCGCAGCTG TCAATGGTTA TGCTCTTGGT 
GGGGGTTGTG AACTTGCCAT GATGTGTGAT ATCATCTATG CTGGCGAGAA AGCCCAGTTC 
GGACAGCCAG AAATCCTCCT GGGGACCATC CCAGGTGCTG GAGGCACTCA GAGACTCACC 
CGAGCAGTCG GCAAATCGCT AGCAATGGAG ATGGTCCTCA CTGGTGACCG CATCTCAGCT 
CAGGATGCAA AGCAGGCAGG TCTTGTAAGC AAGATTTTTC CTGTTGAAAA ACTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAATTCTA AAATCGTAGT AGCCATGGCG 
AAAGAATCTG TGAATGCAGC CTTTGAGATG ACGTTAACAG AAGGAAATAA GCTGGAGAAG 
AGGCTTTTCT ATTCCACCTT TGCCACCGAT GACCGGAGAG AAGGGATGAC TGCATTTGTA 
GAGAAAAGGA AGGCCAACTT CAAAGACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Echs1 ( NM_053119.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444349.1 Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.

Price & Availability↑

CloneID	OMu14909
Clone ID Related Accession (Same CDS sequence)	NM_053119.3 , NM_053119.2
Accession Version	NM_053119.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Mus musculus(house mouse)
Product	enoyl-CoA hydratase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK040391.1. On Apr 6, 2007 this sequence version replaced NM_053119.1. ##Evidence-Data-START## Transcript exon combination :: AK040391.1, BC072658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCCC TGCGTGCTCT GCTGCCCAGA GCCTGTAGCT CACTGTTGTC CTCAGTCCGC 
TGCCCAGAAC TACGGCGCTT CGCCTCGGGT GCTAACTTTC AGTACATCAT CACAGAAAAG 
AAAGGAAAGA ATAGCAGCGT GGGGCTGATC CAGTTGAACC GCCCCAAAGC ACTCAATGCA 
CTTTGCAATG GCCTGATTGA GGAGCTCAAC CAAGCACTGG AGACCTTTGA GCAAGATCCT 
GCTGTGGGTG CCATTGTGCT CACTGGTGGG GATAAGGCCT TTGCAGCTGG AGCTGACATC 
AAGGAAATGC AGAACCGAAC ATTTCAGGAC TGTTACTCCA GCAAGTTCCT GAGCCACTGG 
GACCACATCA CCCGGGTCAA GAAACCGGTC ATCGCAGCTG TCAATGGTTA TGCTCTTGGT 
GGGGGTTGTG AACTTGCCAT GATGTGTGAT ATCATCTATG CTGGCGAGAA AGCCCAGTTC 
GGACAGCCAG AAATCCTCCT GGGGACCATC CCAGGTGCTG GAGGCACTCA GAGACTCACC 
CGAGCAGTCG GCAAATCGCT AGCAATGGAG ATGGTCCTCA CTGGTGACCG CATCTCAGCT 
CAGGATGCAA AGCAGGCAGG TCTTGTAAGC AAGATTTTTC CTGTTGAAAA ACTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAATTCTA AAATCGTAGT AGCCATGGCG 
AAAGAATCTG TGAATGCAGC CTTTGAGATG ACGTTAACAG AAGGAAATAA GCTGGAGAAG 
AGGCTTTTCT ATTCCACCTT TGCCACCGAT GACCGGAGAG AAGGGATGAC TGCATTTGTA 
GAGAAAAGGA AGGCCAACTT CAAAGACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444349.1
CDS	30..902
Translation	MAALRALLPRACSSLLSSVRCPELRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFVEKRKANFKDH

Target ORF information:

RefSeq Version	NM_053119.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053119.2

ORF Insert Sequence:

ATGGCGGCCC TGCGTGCTCT GCTGCCCAGA GCCTGTAGCT CACTGTTGTC CTCAGTCCGC 
TGCCCAGAAC TACGGCGCTT CGCCTCGGGT GCTAACTTTC AGTACATCAT CACAGAAAAG 
AAAGGAAAGA ATAGCAGCGT GGGGCTGATC CAGTTGAACC GCCCCAAAGC ACTCAATGCA 
CTTTGCAATG GCCTGATTGA GGAGCTCAAC CAAGCACTGG AGACCTTTGA GCAAGATCCT 
GCTGTGGGTG CCATTGTGCT CACTGGTGGG GATAAGGCCT TTGCAGCTGG AGCTGACATC 
AAGGAAATGC AGAACCGAAC ATTTCAGGAC TGTTACTCCA GCAAGTTCCT GAGCCACTGG 
GACCACATCA CCCGGGTCAA GAAACCGGTC ATCGCAGCTG TCAATGGTTA TGCTCTTGGT 
GGGGGTTGTG AACTTGCCAT GATGTGTGAT ATCATCTATG CTGGCGAGAA AGCCCAGTTC 
GGACAGCCAG AAATCCTCCT GGGGACCATC CCAGGTGCTG GAGGCACTCA GAGACTCACC 
CGAGCAGTCG GCAAATCGCT AGCAATGGAG ATGGTCCTCA CTGGTGACCG CATCTCAGCT 
CAGGATGCAA AGCAGGCAGG TCTTGTAAGC AAGATTTTTC CTGTTGAAAA ACTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAATTCTA AAATCGTAGT AGCCATGGCG 
AAAGAATCTG TGAATGCAGC CTTTGAGATG ACGTTAACAG AAGGAAATAA GCTGGAGAAG 
AGGCTTTTCT ATTCCACCTT TGCCACCGAT GACCGGAGAG AAGGGATGAC TGCATTTGTA 
GAGAAAAGGA AGGCCAACTT CAAAGACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase ? and Echs1.
Journal of cellular and molecular medicine16(4)950-8(2012 Apr)
Zhu SG,Xi L,Kukreja RC

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Molecular cell23(4)607-18(2006 Aug)
Kim SC,Sprung R,Chen Y,Xu Y,Ball H,Pei J,Cheng T,Kho Y,Xiao H,Xiao L,Grishin NV,White M,Yang XJ,Zhao Y

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Echs1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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