CNOT1 gene cDNA ORF clone, Ornithorhynchus anatinus(platypus)

mRNA	Protein	Name
XM_007671974.1	XP_007670164.1	CCR4-NOT transcription complex subunit 1 isoform X4
XM_007671978.1	XP_007670168.1	CCR4-NOT transcription complex subunit 1 isoform X9
XM_007671979.1	XP_007670169.1	CCR4-NOT transcription complex subunit 1 isoform X10
XM_029075140.1	XP_028930973.1	CCR4-NOT transcription complex subunit 1 isoform X5
XM_029075142.1	XP_028930975.1	CCR4-NOT transcription complex subunit 1 isoform X7
XM_029075139.1	XP_028930972.1	CCR4-NOT transcription complex subunit 1 isoform X4
XM_029075144.1	XP_028930977.1	CCR4-NOT transcription complex subunit 1 isoform X8
XM_007671972.1	XP_007670162.1	CCR4-NOT transcription complex subunit 1 isoform X2
XM_007671975.1	XP_007670165.1	CCR4-NOT transcription complex subunit 1 isoform X6
XM_007671976.1	XP_007670166.1	CCR4-NOT transcription complex subunit 1 isoform X7
XM_007671971.2	XP_007670161.1	CCR4-NOT transcription complex subunit 1 isoform X1
XM_007671971.1	XP_007670161.1	CCR4-NOT transcription complex subunit 1 isoform X1
XM_007671973.1	XP_007670163.1	CCR4-NOT transcription complex subunit 1 isoform X3
XM_029075136.1	XP_028930969.1	CCR4-NOT transcription complex subunit 1 isoform X1
XM_029075146.1	XP_028930979.1	CCR4-NOT transcription complex subunit 1 isoform X10
XM_003431273.2	XP_003431321.1	CCR4-NOT transcription complex subunit 1 isoform X5
XM_007671977.1	XP_007670167.1	CCR4-NOT transcription complex subunit 1 isoform X8
XM_029075137.1	XP_028930970.1	CCR4-NOT transcription complex subunit 1 isoform X2
XM_029075138.1	XP_028930971.1	CCR4-NOT transcription complex subunit 1 isoform X3
XM_029075141.1	XP_028930974.1	CCR4-NOT transcription complex subunit 1 isoform X6
XM_029075145.1	XP_028930978.1	CCR4-NOT transcription complex subunit 1 isoform X9
XM_029075147.1	XP_028930980.1	CCR4-NOT transcription complex subunit 1 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc112956	XM_007671971.2 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112956	XM_029075136.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112957	XM_029075137.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112958	XM_029075138.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112959	XM_029075139.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112960	XM_029075140.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112961	XM_029075141.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112962	XM_029075142.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112963	XM_029075144.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc112964	XM_029075145.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc112965	XM_029075146.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc112966	XM_029075147.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc13651	XM_007671971.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13652	XM_007671972.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13653	XM_007671973.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13654	XM_007671974.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13655	XM_003431273.2 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13656	XM_007671975.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13657	XM_007671976.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13658	XM_007671977.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OOc13659	XM_007671978.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OOc13660	XM_007671979.1 Latest version!	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

CNOT1 ( XM_007671971.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670161.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc112956
Clone ID Related Accession (Same CDS sequence)	XM_007671971.2 , XM_029075136.1
Accession Version	XM_007671971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 29, 2019 this sequence version replaced XM_007671971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCATACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670161.1
CDS	193..7341
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671971.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671971.2

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCATACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075136.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930969.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

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CloneID	OOc112956
Clone ID Related Accession (Same CDS sequence)	XM_007671971.2 , XM_029075136.1
Accession Version	XM_029075136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCATACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930969.1
CDS	224..7372
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075136.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075136.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCATACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075137.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930970.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

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CloneID	OOc112957
Clone ID Related Accession (Same CDS sequence)	XM_029075137.1
Accession Version	XM_029075137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CATACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930970.1
CDS	224..7369
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075137.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075137.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CATACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075138.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930971.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

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CloneID	OOc112958
Clone ID Related Accession (Same CDS sequence)	XM_029075138.1
Accession Version	XM_029075138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGGT AGAGCTTTTG 
GAAAGAAAGA TGCACCAGCA AGGAATCTTG AAGACCGACG ACCTCATAAC AAGGTTCTTT 
CGCCTGTGCA CAGAAATGTG TGTTGAGATC AGCTACCGAG CTCAGGCCGA GCAGCAACAC 
AATCCCGCAG CCAACCCTAC CATGATCCGA GCCAAATGCT ATCATAACCT GGATGCTTTT 
GTGCGACTTA TTGCCCTCCT GGTCAAACAT TCGGGGGAGG CCACCAATAC GGTCACCAAG 
ATAAACCTGC TGAATAAGGT TCTTGGGATC GTGGTGGGCG TCCTCCTGCA GGATCACGAT 
GTTCGGCAAA ACGATTTTCA GCAGCTTCCA TACCACCGTA TTTTCATCAT GTTGCTTTTG 
GAACTCAATG CCCCCGAGCA TGTCTTGGAG ACCATTAATT TTCAGACGCT CACAGCTTTC 
TGCAATACAT TCCACATCTT GAGGCCTACC AAAGCTCCCG GCTTTGTGTA TGCCTGGCTT 
GAGCTGATCT CCCACCGGAT ATTTATCGCA CGGATGCTGG CACATACACC ACAGCAGAAG 
GGCTGGCCCA TGTATGCCCA ACTACTGATT GATTTGTTCA AATATTTGGC TCCCTTCCTT 
AGAAATGTGG AACTTACCAA ACCTATGCAA ATCCTGTACA AGGGTACTTT ACGAGTGCTC 
CTGGTCTTAT TGCATGACTT TCCGGAATTC CTTTGTGATT ACCATTATGG GTTCTGTGAT 
GTCATCCCAC CCAATTGCAT CCAGCTAAGA AATTTGATCC TGAGTGCCTT TCCACGCAAC 
ATGAGGCTCC CAGACCCATT CACTCCCAAC TTAAAGGTGG ATATGTTGAG TGAAATTAAC 
ATCGCTCCTA GAATCCTCAC CAATTTCACA GGAGTGATGC CACCTCAGTT CAAAAAAGAT 
CTGGATTCCT ATCTCAAAAC CCGCTCACCA GTCACGTTCC TTTCGGACTT GCGCAGCAAC 
CTGCAGGTAT CGAATGAACC CGGTAATCGT TACAACATCC AGTTGATCAA CGCCCTGGTG 
CTCTACGTAG GTACTCAAGC CATCGCCCAC ATCCACAATA AAGGTAGCAC GCCATCCATG 
AGCACCATCA CCCACTCGGC TCACATGGAC ATCTTCCAGA ACCTGGCCGT GGACCTGGAC 
ACTGAAGGTC GGTACCTGTT CCTGAATGCC ATCGCTAACC AGCTGCGGTA CCCGAATAGC 
CACACTCATT ACTTCAGTTG TACCATGCTG TACCTGTTCG CAGAAGCCAA CACTGAAGCC 
ATCCAGGAAC AGATCACCAG GGTTCTCTTG GAACGGTTGA TTGTAAATAG GCCACATCCT 
TGGGGTCTCC TCATTACCTT CATCGAGCTG ATTAAAAACC CAGCGTTTAA GTTCTGGAAC 
CACGAGTTCG TTCACTGTGC CCCAGAAATT GAAAAGTTGT TCCAGTCGGT TGCACAATGC 
TGCATGGGGC AGAAGCAGGC TCAGCAAGTG ATGGAAGGGA CCGGTGCCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930971.1
CDS	224..7357
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075138.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075138.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGGT AGAGCTTTTG 
GAAAGAAAGA TGCACCAGCA AGGAATCTTG AAGACCGACG ACCTCATAAC AAGGTTCTTT 
CGCCTGTGCA CAGAAATGTG TGTTGAGATC AGCTACCGAG CTCAGGCCGA GCAGCAACAC 
AATCCCGCAG CCAACCCTAC CATGATCCGA GCCAAATGCT ATCATAACCT GGATGCTTTT 
GTGCGACTTA TTGCCCTCCT GGTCAAACAT TCGGGGGAGG CCACCAATAC GGTCACCAAG 
ATAAACCTGC TGAATAAGGT TCTTGGGATC GTGGTGGGCG TCCTCCTGCA GGATCACGAT 
GTTCGGCAAA ACGATTTTCA GCAGCTTCCA TACCACCGTA TTTTCATCAT GTTGCTTTTG 
GAACTCAATG CCCCCGAGCA TGTCTTGGAG ACCATTAATT TTCAGACGCT CACAGCTTTC 
TGCAATACAT TCCACATCTT GAGGCCTACC AAAGCTCCCG GCTTTGTGTA TGCCTGGCTT 
GAGCTGATCT CCCACCGGAT ATTTATCGCA CGGATGCTGG CACATACACC ACAGCAGAAG 
GGCTGGCCCA TGTATGCCCA ACTACTGATT GATTTGTTCA AATATTTGGC TCCCTTCCTT 
AGAAATGTGG AACTTACCAA ACCTATGCAA ATCCTGTACA AGGGTACTTT ACGAGTGCTC 
CTGGTCTTAT TGCATGACTT TCCGGAATTC CTTTGTGATT ACCATTATGG GTTCTGTGAT 
GTCATCCCAC CCAATTGCAT CCAGCTAAGA AATTTGATCC TGAGTGCCTT TCCACGCAAC 
ATGAGGCTCC CAGACCCATT CACTCCCAAC TTAAAGGTGG ATATGTTGAG TGAAATTAAC 
ATCGCTCCTA GAATCCTCAC CAATTTCACA GGAGTGATGC CACCTCAGTT CAAAAAAGAT 
CTGGATTCCT ATCTCAAAAC CCGCTCACCA GTCACGTTCC TTTCGGACTT GCGCAGCAAC 
CTGCAGGTAT CGAATGAACC CGGTAATCGT TACAACATCC AGTTGATCAA CGCCCTGGTG 
CTCTACGTAG GTACTCAAGC CATCGCCCAC ATCCACAATA AAGGTAGCAC GCCATCCATG 
AGCACCATCA CCCACTCGGC TCACATGGAC ATCTTCCAGA ACCTGGCCGT GGACCTGGAC 
ACTGAAGGTC GGTACCTGTT CCTGAATGCC ATCGCTAACC AGCTGCGGTA CCCGAATAGC 
CACACTCATT ACTTCAGTTG TACCATGCTG TACCTGTTCG CAGAAGCCAA CACTGAAGCC 
ATCCAGGAAC AGATCACCAG GGTTCTCTTG GAACGGTTGA TTGTAAATAG GCCACATCCT 
TGGGGTCTCC TCATTACCTT CATCGAGCTG ATTAAAAACC CAGCGTTTAA GTTCTGGAAC 
CACGAGTTCG TTCACTGTGC CCCAGAAATT GAAAAGTTGT TCCAGTCGGT TGCACAATGC 
TGCATGGGGC AGAAGCAGGC TCAGCAAGTG ATGGAAGGGA CCGGTGCCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075139.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930972.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

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CloneID	OOc112959
Clone ID Related Accession (Same CDS sequence)	XM_029075139.1
Accession Version	XM_029075139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA 
AGAAAGATGC ACCAGCAAGG AATCTTGAAG ACCGACGACC TCATAACAAG GTTCTTTCGC 
CTGTGCACAG AAATGTGTGT TGAGATCAGC TACCGAGCTC AGGCCGAGCA GCAACACAAT 
CCCGCAGCCA ACCCTACCAT GATCCGAGCC AAATGCTATC ATAACCTGGA TGCTTTTGTG 
CGACTTATTG CCCTCCTGGT CAAACATTCG GGGGAGGCCA CCAATACGGT CACCAAGATA 
AACCTGCTGA ATAAGGTTCT TGGGATCGTG GTGGGCGTCC TCCTGCAGGA TCACGATGTT 
CGGCAAAACG ATTTTCAGCA GCTTCCATAC CACCGTATTT TCATCATGTT GCTTTTGGAA 
CTCAATGCCC CCGAGCATGT CTTGGAGACC ATTAATTTTC AGACGCTCAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCCGGCT TTGTGTATGC CTGGCTTGAG 
CTGATCTCCC ACCGGATATT TATCGCACGG ATGCTGGCAC ATACACCACA GCAGAAGGGC 
TGGCCCATGT ATGCCCAACT ACTGATTGAT TTGTTCAAAT ATTTGGCTCC CTTCCTTAGA 
AATGTGGAAC TTACCAAACC TATGCAAATC CTGTACAAGG GTACTTTACG AGTGCTCCTG 
GTCTTATTGC ATGACTTTCC GGAATTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTC 
ATCCCACCCA ATTGCATCCA GCTAAGAAAT TTGATCCTGA GTGCCTTTCC ACGCAACATG 
AGGCTCCCAG ACCCATTCAC TCCCAACTTA AAGGTGGATA TGTTGAGTGA AATTAACATC 
GCTCCTAGAA TCCTCACCAA TTTCACAGGA GTGATGCCAC CTCAGTTCAA AAAAGATCTG 
GATTCCTATC TCAAAACCCG CTCACCAGTC ACGTTCCTTT CGGACTTGCG CAGCAACCTG 
CAGGTATCGA ATGAACCCGG TAATCGTTAC AACATCCAGT TGATCAACGC CCTGGTGCTC 
TACGTAGGTA CTCAAGCCAT CGCCCACATC CACAATAAAG GTAGCACGCC ATCCATGAGC 
ACCATCACCC ACTCGGCTCA CATGGACATC TTCCAGAACC TGGCCGTGGA CCTGGACACT 
GAAGGTCGGT ACCTGTTCCT GAATGCCATC GCTAACCAGC TGCGGTACCC GAATAGCCAC 
ACTCATTACT TCAGTTGTAC CATGCTGTAC CTGTTCGCAG AAGCCAACAC TGAAGCCATC 
CAGGAACAGA TCACCAGGGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTCCTCA TTACCTTCAT CGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAC 
GAGTTCGTTC ACTGTGCCCC AGAAATTGAA AAGTTGTTCC AGTCGGTTGC ACAATGCTGC 
ATGGGGCAGA AGCAGGCTCA GCAAGTGATG GAAGGGACCG GTGCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930972.1
CDS	224..7354
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075139.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075139.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA 
AGAAAGATGC ACCAGCAAGG AATCTTGAAG ACCGACGACC TCATAACAAG GTTCTTTCGC 
CTGTGCACAG AAATGTGTGT TGAGATCAGC TACCGAGCTC AGGCCGAGCA GCAACACAAT 
CCCGCAGCCA ACCCTACCAT GATCCGAGCC AAATGCTATC ATAACCTGGA TGCTTTTGTG 
CGACTTATTG CCCTCCTGGT CAAACATTCG GGGGAGGCCA CCAATACGGT CACCAAGATA 
AACCTGCTGA ATAAGGTTCT TGGGATCGTG GTGGGCGTCC TCCTGCAGGA TCACGATGTT 
CGGCAAAACG ATTTTCAGCA GCTTCCATAC CACCGTATTT TCATCATGTT GCTTTTGGAA 
CTCAATGCCC CCGAGCATGT CTTGGAGACC ATTAATTTTC AGACGCTCAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCCGGCT TTGTGTATGC CTGGCTTGAG 
CTGATCTCCC ACCGGATATT TATCGCACGG ATGCTGGCAC ATACACCACA GCAGAAGGGC 
TGGCCCATGT ATGCCCAACT ACTGATTGAT TTGTTCAAAT ATTTGGCTCC CTTCCTTAGA 
AATGTGGAAC TTACCAAACC TATGCAAATC CTGTACAAGG GTACTTTACG AGTGCTCCTG 
GTCTTATTGC ATGACTTTCC GGAATTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTC 
ATCCCACCCA ATTGCATCCA GCTAAGAAAT TTGATCCTGA GTGCCTTTCC ACGCAACATG 
AGGCTCCCAG ACCCATTCAC TCCCAACTTA AAGGTGGATA TGTTGAGTGA AATTAACATC 
GCTCCTAGAA TCCTCACCAA TTTCACAGGA GTGATGCCAC CTCAGTTCAA AAAAGATCTG 
GATTCCTATC TCAAAACCCG CTCACCAGTC ACGTTCCTTT CGGACTTGCG CAGCAACCTG 
CAGGTATCGA ATGAACCCGG TAATCGTTAC AACATCCAGT TGATCAACGC CCTGGTGCTC 
TACGTAGGTA CTCAAGCCAT CGCCCACATC CACAATAAAG GTAGCACGCC ATCCATGAGC 
ACCATCACCC ACTCGGCTCA CATGGACATC TTCCAGAACC TGGCCGTGGA CCTGGACACT 
GAAGGTCGGT ACCTGTTCCT GAATGCCATC GCTAACCAGC TGCGGTACCC GAATAGCCAC 
ACTCATTACT TCAGTTGTAC CATGCTGTAC CTGTTCGCAG AAGCCAACAC TGAAGCCATC 
CAGGAACAGA TCACCAGGGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTCCTCA TTACCTTCAT CGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAC 
GAGTTCGTTC ACTGTGCCCC AGAAATTGAA AAGTTGTTCC AGTCGGTTGC ACAATGCTGC 
ATGGGGCAGA AGCAGGCTCA GCAAGTGATG GAAGGGACCG GTGCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075140.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930973.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

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CloneID	OOc112960
Clone ID Related Accession (Same CDS sequence)	XM_029075140.1
Accession Version	XM_029075140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGATGCACC AGCAAGGAAT CTTGAAGACC GACGACCTCA TAACAAGGTT CTTTCGCCTG 
TGCACAGAAA TGTGTGTTGA GATCAGCTAC CGAGCTCAGG CCGAGCAGCA ACACAATCCC 
GCAGCCAACC CTACCATGAT CCGAGCCAAA TGCTATCATA ACCTGGATGC TTTTGTGCGA 
CTTATTGCCC TCCTGGTCAA ACATTCGGGG GAGGCCACCA ATACGGTCAC CAAGATAAAC 
CTGCTGAATA AGGTTCTTGG GATCGTGGTG GGCGTCCTCC TGCAGGATCA CGATGTTCGG 
CAAAACGATT TTCAGCAGCT TCCATACCAC CGTATTTTCA TCATGTTGCT TTTGGAACTC 
AATGCCCCCG AGCATGTCTT GGAGACCATT AATTTTCAGA CGCTCACAGC TTTCTGCAAT 
ACATTCCACA TCTTGAGGCC TACCAAAGCT CCCGGCTTTG TGTATGCCTG GCTTGAGCTG 
ATCTCCCACC GGATATTTAT CGCACGGATG CTGGCACATA CACCACAGCA GAAGGGCTGG 
CCCATGTATG CCCAACTACT GATTGATTTG TTCAAATATT TGGCTCCCTT CCTTAGAAAT 
GTGGAACTTA CCAAACCTAT GCAAATCCTG TACAAGGGTA CTTTACGAGT GCTCCTGGTC 
TTATTGCATG ACTTTCCGGA ATTCCTTTGT GATTACCATT ATGGGTTCTG TGATGTCATC 
CCACCCAATT GCATCCAGCT AAGAAATTTG ATCCTGAGTG CCTTTCCACG CAACATGAGG 
CTCCCAGACC CATTCACTCC CAACTTAAAG GTGGATATGT TGAGTGAAAT TAACATCGCT 
CCTAGAATCC TCACCAATTT CACAGGAGTG ATGCCACCTC AGTTCAAAAA AGATCTGGAT 
TCCTATCTCA AAACCCGCTC ACCAGTCACG TTCCTTTCGG ACTTGCGCAG CAACCTGCAG 
GTATCGAATG AACCCGGTAA TCGTTACAAC ATCCAGTTGA TCAACGCCCT GGTGCTCTAC 
GTAGGTACTC AAGCCATCGC CCACATCCAC AATAAAGGTA GCACGCCATC CATGAGCACC 
ATCACCCACT CGGCTCACAT GGACATCTTC CAGAACCTGG CCGTGGACCT GGACACTGAA 
GGTCGGTACC TGTTCCTGAA TGCCATCGCT AACCAGCTGC GGTACCCGAA TAGCCACACT 
CATTACTTCA GTTGTACCAT GCTGTACCTG TTCGCAGAAG CCAACACTGA AGCCATCCAG 
GAACAGATCA CCAGGGTTCT CTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT 
CTCCTCATTA CCTTCATCGA GCTGATTAAA AACCCAGCGT TTAAGTTCTG GAACCACGAG 
TTCGTTCACT GTGCCCCAGA AATTGAAAAG TTGTTCCAGT CGGTTGCACA ATGCTGCATG 
GGGCAGAAGC AGGCTCAGCA AGTGATGGAA GGGACCGGTG CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930973.1
CDS	224..7351
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075140.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075140.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGATGCACC AGCAAGGAAT CTTGAAGACC GACGACCTCA TAACAAGGTT CTTTCGCCTG 
TGCACAGAAA TGTGTGTTGA GATCAGCTAC CGAGCTCAGG CCGAGCAGCA ACACAATCCC 
GCAGCCAACC CTACCATGAT CCGAGCCAAA TGCTATCATA ACCTGGATGC TTTTGTGCGA 
CTTATTGCCC TCCTGGTCAA ACATTCGGGG GAGGCCACCA ATACGGTCAC CAAGATAAAC 
CTGCTGAATA AGGTTCTTGG GATCGTGGTG GGCGTCCTCC TGCAGGATCA CGATGTTCGG 
CAAAACGATT TTCAGCAGCT TCCATACCAC CGTATTTTCA TCATGTTGCT TTTGGAACTC 
AATGCCCCCG AGCATGTCTT GGAGACCATT AATTTTCAGA CGCTCACAGC TTTCTGCAAT 
ACATTCCACA TCTTGAGGCC TACCAAAGCT CCCGGCTTTG TGTATGCCTG GCTTGAGCTG 
ATCTCCCACC GGATATTTAT CGCACGGATG CTGGCACATA CACCACAGCA GAAGGGCTGG 
CCCATGTATG CCCAACTACT GATTGATTTG TTCAAATATT TGGCTCCCTT CCTTAGAAAT 
GTGGAACTTA CCAAACCTAT GCAAATCCTG TACAAGGGTA CTTTACGAGT GCTCCTGGTC 
TTATTGCATG ACTTTCCGGA ATTCCTTTGT GATTACCATT ATGGGTTCTG TGATGTCATC 
CCACCCAATT GCATCCAGCT AAGAAATTTG ATCCTGAGTG CCTTTCCACG CAACATGAGG 
CTCCCAGACC CATTCACTCC CAACTTAAAG GTGGATATGT TGAGTGAAAT TAACATCGCT 
CCTAGAATCC TCACCAATTT CACAGGAGTG ATGCCACCTC AGTTCAAAAA AGATCTGGAT 
TCCTATCTCA AAACCCGCTC ACCAGTCACG TTCCTTTCGG ACTTGCGCAG CAACCTGCAG 
GTATCGAATG AACCCGGTAA TCGTTACAAC ATCCAGTTGA TCAACGCCCT GGTGCTCTAC 
GTAGGTACTC AAGCCATCGC CCACATCCAC AATAAAGGTA GCACGCCATC CATGAGCACC 
ATCACCCACT CGGCTCACAT GGACATCTTC CAGAACCTGG CCGTGGACCT GGACACTGAA 
GGTCGGTACC TGTTCCTGAA TGCCATCGCT AACCAGCTGC GGTACCCGAA TAGCCACACT 
CATTACTTCA GTTGTACCAT GCTGTACCTG TTCGCAGAAG CCAACACTGA AGCCATCCAG 
GAACAGATCA CCAGGGTTCT CTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT 
CTCCTCATTA CCTTCATCGA GCTGATTAAA AACCCAGCGT TTAAGTTCTG GAACCACGAG 
TTCGTTCACT GTGCCCCAGA AATTGAAAAG TTGTTCCAGT CGGTTGCACA ATGCTGCATG 
GGGCAGAAGC AGGCTCAGCA AGTGATGGAA GGGACCGGTG CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075141.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930974.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.

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CloneID	OOc112961
Clone ID Related Accession (Same CDS sequence)	XM_029075141.1
Accession Version	XM_029075141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
ATGCACCAGC AAGGAATCTT GAAGACCGAC GACCTCATAA CAAGGTTCTT TCGCCTGTGC 
ACAGAAATGT GTGTTGAGAT CAGCTACCGA GCTCAGGCCG AGCAGCAACA CAATCCCGCA 
GCCAACCCTA CCATGATCCG AGCCAAATGC TATCATAACC TGGATGCTTT TGTGCGACTT 
ATTGCCCTCC TGGTCAAACA TTCGGGGGAG GCCACCAATA CGGTCACCAA GATAAACCTG 
CTGAATAAGG TTCTTGGGAT CGTGGTGGGC GTCCTCCTGC AGGATCACGA TGTTCGGCAA 
AACGATTTTC AGCAGCTTCC ATACCACCGT ATTTTCATCA TGTTGCTTTT GGAACTCAAT 
GCCCCCGAGC ATGTCTTGGA GACCATTAAT TTTCAGACGC TCACAGCTTT CTGCAATACA 
TTCCACATCT TGAGGCCTAC CAAAGCTCCC GGCTTTGTGT ATGCCTGGCT TGAGCTGATC 
TCCCACCGGA TATTTATCGC ACGGATGCTG GCACATACAC CACAGCAGAA GGGCTGGCCC 
ATGTATGCCC AACTACTGAT TGATTTGTTC AAATATTTGG CTCCCTTCCT TAGAAATGTG 
GAACTTACCA AACCTATGCA AATCCTGTAC AAGGGTACTT TACGAGTGCT CCTGGTCTTA 
TTGCATGACT TTCCGGAATT CCTTTGTGAT TACCATTATG GGTTCTGTGA TGTCATCCCA 
CCCAATTGCA TCCAGCTAAG AAATTTGATC CTGAGTGCCT TTCCACGCAA CATGAGGCTC 
CCAGACCCAT TCACTCCCAA CTTAAAGGTG GATATGTTGA GTGAAATTAA CATCGCTCCT 
AGAATCCTCA CCAATTTCAC AGGAGTGATG CCACCTCAGT TCAAAAAAGA TCTGGATTCC 
TATCTCAAAA CCCGCTCACC AGTCACGTTC CTTTCGGACT TGCGCAGCAA CCTGCAGGTA 
TCGAATGAAC CCGGTAATCG TTACAACATC CAGTTGATCA ACGCCCTGGT GCTCTACGTA 
GGTACTCAAG CCATCGCCCA CATCCACAAT AAAGGTAGCA CGCCATCCAT GAGCACCATC 
ACCCACTCGG CTCACATGGA CATCTTCCAG AACCTGGCCG TGGACCTGGA CACTGAAGGT 
CGGTACCTGT TCCTGAATGC CATCGCTAAC CAGCTGCGGT ACCCGAATAG CCACACTCAT 
TACTTCAGTT GTACCATGCT GTACCTGTTC GCAGAAGCCA ACACTGAAGC CATCCAGGAA 
CAGATCACCA GGGTTCTCTT GGAACGGTTG ATTGTAAATA GGCCACATCC TTGGGGTCTC 
CTCATTACCT TCATCGAGCT GATTAAAAAC CCAGCGTTTA AGTTCTGGAA CCACGAGTTC 
GTTCACTGTG CCCCAGAAAT TGAAAAGTTG TTCCAGTCGG TTGCACAATG CTGCATGGGG 
CAGAAGCAGG CTCAGCAAGT GATGGAAGGG ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930974.1
CDS	224..7348
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075141.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075141.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
ATGCACCAGC AAGGAATCTT GAAGACCGAC GACCTCATAA CAAGGTTCTT TCGCCTGTGC 
ACAGAAATGT GTGTTGAGAT CAGCTACCGA GCTCAGGCCG AGCAGCAACA CAATCCCGCA 
GCCAACCCTA CCATGATCCG AGCCAAATGC TATCATAACC TGGATGCTTT TGTGCGACTT 
ATTGCCCTCC TGGTCAAACA TTCGGGGGAG GCCACCAATA CGGTCACCAA GATAAACCTG 
CTGAATAAGG TTCTTGGGAT CGTGGTGGGC GTCCTCCTGC AGGATCACGA TGTTCGGCAA 
AACGATTTTC AGCAGCTTCC ATACCACCGT ATTTTCATCA TGTTGCTTTT GGAACTCAAT 
GCCCCCGAGC ATGTCTTGGA GACCATTAAT TTTCAGACGC TCACAGCTTT CTGCAATACA 
TTCCACATCT TGAGGCCTAC CAAAGCTCCC GGCTTTGTGT ATGCCTGGCT TGAGCTGATC 
TCCCACCGGA TATTTATCGC ACGGATGCTG GCACATACAC CACAGCAGAA GGGCTGGCCC 
ATGTATGCCC AACTACTGAT TGATTTGTTC AAATATTTGG CTCCCTTCCT TAGAAATGTG 
GAACTTACCA AACCTATGCA AATCCTGTAC AAGGGTACTT TACGAGTGCT CCTGGTCTTA 
TTGCATGACT TTCCGGAATT CCTTTGTGAT TACCATTATG GGTTCTGTGA TGTCATCCCA 
CCCAATTGCA TCCAGCTAAG AAATTTGATC CTGAGTGCCT TTCCACGCAA CATGAGGCTC 
CCAGACCCAT TCACTCCCAA CTTAAAGGTG GATATGTTGA GTGAAATTAA CATCGCTCCT 
AGAATCCTCA CCAATTTCAC AGGAGTGATG CCACCTCAGT TCAAAAAAGA TCTGGATTCC 
TATCTCAAAA CCCGCTCACC AGTCACGTTC CTTTCGGACT TGCGCAGCAA CCTGCAGGTA 
TCGAATGAAC CCGGTAATCG TTACAACATC CAGTTGATCA ACGCCCTGGT GCTCTACGTA 
GGTACTCAAG CCATCGCCCA CATCCACAAT AAAGGTAGCA CGCCATCCAT GAGCACCATC 
ACCCACTCGG CTCACATGGA CATCTTCCAG AACCTGGCCG TGGACCTGGA CACTGAAGGT 
CGGTACCTGT TCCTGAATGC CATCGCTAAC CAGCTGCGGT ACCCGAATAG CCACACTCAT 
TACTTCAGTT GTACCATGCT GTACCTGTTC GCAGAAGCCA ACACTGAAGC CATCCAGGAA 
CAGATCACCA GGGTTCTCTT GGAACGGTTG ATTGTAAATA GGCCACATCC TTGGGGTCTC 
CTCATTACCT TCATCGAGCT GATTAAAAAC CCAGCGTTTA AGTTCTGGAA CCACGAGTTC 
GTTCACTGTG CCCCAGAAAT TGAAAAGTTG TTCCAGTCGG TTGCACAATG CTGCATGGGG 
CAGAAGCAGG CTCAGCAAGT GATGGAAGGG ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075142.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930975.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.

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CloneID	OOc112962
Clone ID Related Accession (Same CDS sequence)	XM_029075142.1
Accession Version	XM_029075142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCAT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930975.1
CDS	224..7336
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075142.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075142.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCAT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075144.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930977.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.

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CloneID	OOc112963
Clone ID Related Accession (Same CDS sequence)	XM_029075144.1
Accession Version	XM_029075144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGAGCCC TTCATCCAGG CGTGTATGAC TTTCTTAAAG 
AGACGGTGCC CTTCTATCTT GGGTGGCCTT GCCCCAGAGA AGGATCAGCC CAAAAGCGCC 
CAACTTCCTC CAGAAACACT GGCAACAATG TTGGCCTGTC TTCAAGCGTG TGCCGGGAGT 
GTTTCTCAAG AGCTTTCGGA GACTATCCTC ACTATGGTGG CCAACTGCAG CAATGTTATG 
AACAAGGCTA GACAGCCACC CCCCGGAGTC ATGCCAAAAG GACGCCCTCC CAGTGCAAGC 
AGCTTAGACG CCATTTCTCC CGTACAGATT GACTCTCTTG GTGGGATGGC ATCTCTTAGT 
ATAGGTGGTT CAGCTGCCCC CCACACCCAG AGTATGCAGG GTTTTCCTCC AAATTTGGGT 
TCTGCCTTCA GTACCCCTCA GTCACCAGCA AAAGCATTTC CACCCCTTTC TACCCCAAAT 
CAGACCACAG CTTTCAGCGG TATCGGAGGG CTCTCTTCAC AACTTCCAGT AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CATACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930977.1
CDS	224..7249
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075144.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075144.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGAGCCC TTCATCCAGG CGTGTATGAC TTTCTTAAAG 
AGACGGTGCC CTTCTATCTT GGGTGGCCTT GCCCCAGAGA AGGATCAGCC CAAAAGCGCC 
CAACTTCCTC CAGAAACACT GGCAACAATG TTGGCCTGTC TTCAAGCGTG TGCCGGGAGT 
GTTTCTCAAG AGCTTTCGGA GACTATCCTC ACTATGGTGG CCAACTGCAG CAATGTTATG 
AACAAGGCTA GACAGCCACC CCCCGGAGTC ATGCCAAAAG GACGCCCTCC CAGTGCAAGC 
AGCTTAGACG CCATTTCTCC CGTACAGATT GACTCTCTTG GTGGGATGGC ATCTCTTAGT 
ATAGGTGGTT CAGCTGCCCC CCACACCCAG AGTATGCAGG GTTTTCCTCC AAATTTGGGT 
TCTGCCTTCA GTACCCCTCA GTCACCAGCA AAAGCATTTC CACCCCTTTC TACCCCAAAT 
CAGACCACAG CTTTCAGCGG TATCGGAGGG CTCTCTTCAC AACTTCCAGT AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CATACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075145.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930978.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.

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CloneID	OOc112964
Clone ID Related Accession (Same CDS sequence)	XM_029075145.1
Accession Version	XM_029075145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGTAGG TGGTCTCGGT ACAGGCAGCC TGACCGGTAT AGGAACTGCC 
GCTCTTGGGC TCCCCGCAGT GAATAATGAT CCGTTTGTGC AGAGGAAACT GGGCACATCT 
GGACTGAACC AGCCTACATT CCAGCAGAGT AAGATGAAAC CTTCGGACTT GTCTCAGGTG 
TGGCCAGAGG CAAATCAGCA CTTTAGCAAA GAGATAGATG ATGAAGCAAA CAGCTATTTT 
CAGCGTATTT ATAACCATCC ACCACACCCA ACCATGTCTG TTGATGAGGT TTTAGAAATG 
CTACAGAGGT TTAAAGACTC CAACATAAAA CGGGAACGAG AAGTGTTTAA CTGTATGTTG 
AGGAACTTGT TTGAAGAGTA TCGGTTTTTC CCCCAGTACC CAGATAAAGA GCTGCATATA 
ACAGCCTGCC TCTTTGGTGG GATAATAGAA AAAGGACTGG TCACTTACAT GGCTCTGGGT 
CTTGCCCTGC GCTATGTTCT TGAAGCCTTA CGCAAGCCGT TTGCATCCAA AATGTATTAT 
TTTGGAATCG CTGCACTAGA TAGGTTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT 
CAGCACTTGG CTTCTATTAG TCACTTTATA CAGTTTCCTC ATCATCTACA GGAGTACATT 
GAATACGGAC AGCAATCTAG AGACCCTCCT GTAAAAATGC AGGGTTCCAT CACCACCCCT 
GGAAGTATTG CACTTGCCCA GGCTCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCTCTT 
GCTGGTCAAG TCAGCACTAT AGTAACCACC TCTACCACCA CCACTGCTAA AACCATCACG 
ATCACCCGAC CAACAGGAGT CAGCTTCAAA AAGGATGTGC CACCTTCTAT TAATACTACC 
AACATAGATA CACTGCTAGT GGCGACCGAT CAAACTGAGA GAATTGTGGA ACCACCAGAA 
AATGTGCAAG AGAAAATTGC TTTCATCTTC AACAATCTCT CTCAATCAAA CATGACACAG 
AAGGTTGAGG AGCTGAAAGA AACGGTAAAA GAGGAATTTA TGCCTTGGGT TTCACAATAC 
CTGGTAATGA AGAGAGTCAG TATTGAGCCA AACTTCCACA GCTTGTATTC AAATTTCCTC 
GATACTCTTA AGAACCCTGA ATTTAACAAG ATGGTTCTGA ATGAAACCTA CAGAAACATC 
AAAGTGCTAC TGACATCAGA TAAAGCTGCA GCCAATTTTT CAGACCGTTC CCTACTGAAG 
AACCTGGGCC ACTGGCTGGG AATGATTACA CTAGCTAAAA ATAAACCCAT CTTGCACACT 
GACTTGGATG TAAAATCTTT GCTGCTCGAA GCTTACGTTA AAGGACAACA AGAATTGCTT 
TATGTGGTGC CCTTTGTTGC CAAAGTACTG GAATCCAGTG TTCGAAGTGT GGTGTTCAGG 
CCACCAAACC CCTGGACTAT GGCTATCATG AACGTCTTAG CCGAGTTACA TCAAGAACAT 
GACCTGAAGC TAAACTTGAA ATTTGAAATT GAGGTGCTCT GCAAGAACCT TGCGTTAGAC 
ATCAGTGAGC TAAAACCCGG GAACCTTCTG AAGGATAAAG ATCGATTAAA AAACTTAGAA 
GAACAGCTCT CTGCTCCAAA GAAAGATGTA AAACAACCAG AAGAACTTCC AGCCATCACA 
ACAACTACGG CCTCTACAAC ACCAGCCACC AGTACGACCT GTACGGCGAC AGTTCCTCCT 
CAGCCCCAGT ACAGCTACCA CGACATCAAC GTGTACTCGC TCGGAGGGCT GGCCCCACAC 
ATTACTCTAA ATCCCACGAT TCCGTTGTTC CAGGCCCATC CACAGTTAAA GCAGTGTGTG 
CGCCAAGCAA TCGAACGTGC TGTACAAGAG CTTGTCCATC CGGTGGTGGA TAGGTCCATT 
AAGATTGCCA TGACTACTTG TGAACAAATA GTCAGGAAGG ATTTTGCACT GGATTCAGAG 
GAGTCACGCA TGCGAATAGC AGCACATCAC ATGATGAGGA ATCTGACAGC CGGAATGGCT 
ATGATAACTT GCAGGGAACC TTTGCTGATG AGTATAGCTA CCAACCTGAA AAACAGCTTC 
GCCACCGCAC TGCGGGCTGC GTCTCCTCAG CAAAGGGAAA TGATGGAGCA GGCAGCTGCG 
CAGTTAGCTC AGGACAATTG TGAATTAGCC TGCTGCTTCA TTCAGAAGAC TGCCGTAGAA 
AAAGCAGGCC CCGAAATGGA CAAGAGGCTA GCAACTGAGT TTGAGCTGCG CAAACACGCT 
AGGCAGGAGG GCCGTAGGTA CTGTGATCCC GTTGTATTAA CCTATCAGGC TGAGCGGATG 
CCAGAACAGA TCAGGCTGAA GGTAGGAGGT GTGGACCCCA AACAGCTGGC TGTTTATGAG 
GAGTTTGCAC GCAATGTTCC TGGCTTCTTG CCTACTAATG ATTTAACCCA GCCCACGGGA 
TTCTTAGCGC AGCCTATGAA ACAAGCTTGG GCAACAGACG ATGTAGCCCA GATTTATGAC 
AAGTGTATTA CAGAACTGGA ACAGCACCTG CATGCCATTC CACCTGCATT GGCCATGAAC 
CCTCAAGCTC AGGCTCTTCG CAGCCTTTTG GAAGCTGTGG TGCTGTCTCG CAACTCTCGT 
GATGCCATAG CGGCACTTGG GTTGCTTCAG AAGGCAGTAG AGGGTTTACT GGATGCCACA 
AGCGGTGCAG ATGCCGACCT ATTGCTACGT TACCGAGAGT GTCATCTACT TGTACTGAAA 
GCGCTGCAGG ATGGTCGGGC CTATGGTTCA CCGTGGTGTA ATAAACAGAT CACGAGGTGC 
CTGATCGAGT GTCGGGATGA ATATAAGTAT AACGTTGAAG CAGTGGAACT GCTGATTCGG 
AACCATCTAG TTAACATGCA GCAGTATGAC CTGCACCTGG CTCAGTCAAT GGAGAATGGT 
TTGAACTACA TGGCAGTGGC ATTTGCTATG CAGTTGGTGA AGATCCTGTT GGTGGATGAA 
AGGACTGTAG CCCATGTAAC AGAGGCAGAT TTATTTCACA CCATTGAAAC TCTGATGAGG 
ATTAATGCTC ACTCCAGAGG AAATGCCCCC GAAGGATTAC CCCAGCTGAT GGAAGTGGTG 
CGTGCCAACT ATGAAGCAAT GATTGATCGT GCTCATGGAG GTCCTAACTT CATGATGCAC 
TCTGGGATCT CTCAAGCCTC AGAATATGAT GACCCTCCAG GCCTAAGGGA GAAGGCAGAG 
TATCTTCTGA GGGAATGGGT GAATCTCTAC CATTCAGCCG CAGCTGGTCG CGACAGTACC 
AAAGCTTTCT CTGCGTTTGT TGGACAGGTA GAGCTTTTGG AAAGAAAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCAT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930978.1
CDS	224..7216
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075145.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075145.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGTAGG TGGTCTCGGT ACAGGCAGCC TGACCGGTAT AGGAACTGCC 
GCTCTTGGGC TCCCCGCAGT GAATAATGAT CCGTTTGTGC AGAGGAAACT GGGCACATCT 
GGACTGAACC AGCCTACATT CCAGCAGAGT AAGATGAAAC CTTCGGACTT GTCTCAGGTG 
TGGCCAGAGG CAAATCAGCA CTTTAGCAAA GAGATAGATG ATGAAGCAAA CAGCTATTTT 
CAGCGTATTT ATAACCATCC ACCACACCCA ACCATGTCTG TTGATGAGGT TTTAGAAATG 
CTACAGAGGT TTAAAGACTC CAACATAAAA CGGGAACGAG AAGTGTTTAA CTGTATGTTG 
AGGAACTTGT TTGAAGAGTA TCGGTTTTTC CCCCAGTACC CAGATAAAGA GCTGCATATA 
ACAGCCTGCC TCTTTGGTGG GATAATAGAA AAAGGACTGG TCACTTACAT GGCTCTGGGT 
CTTGCCCTGC GCTATGTTCT TGAAGCCTTA CGCAAGCCGT TTGCATCCAA AATGTATTAT 
TTTGGAATCG CTGCACTAGA TAGGTTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT 
CAGCACTTGG CTTCTATTAG TCACTTTATA CAGTTTCCTC ATCATCTACA GGAGTACATT 
GAATACGGAC AGCAATCTAG AGACCCTCCT GTAAAAATGC AGGGTTCCAT CACCACCCCT 
GGAAGTATTG CACTTGCCCA GGCTCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCTCTT 
GCTGGTCAAG TCAGCACTAT AGTAACCACC TCTACCACCA CCACTGCTAA AACCATCACG 
ATCACCCGAC CAACAGGAGT CAGCTTCAAA AAGGATGTGC CACCTTCTAT TAATACTACC 
AACATAGATA CACTGCTAGT GGCGACCGAT CAAACTGAGA GAATTGTGGA ACCACCAGAA 
AATGTGCAAG AGAAAATTGC TTTCATCTTC AACAATCTCT CTCAATCAAA CATGACACAG 
AAGGTTGAGG AGCTGAAAGA AACGGTAAAA GAGGAATTTA TGCCTTGGGT TTCACAATAC 
CTGGTAATGA AGAGAGTCAG TATTGAGCCA AACTTCCACA GCTTGTATTC AAATTTCCTC 
GATACTCTTA AGAACCCTGA ATTTAACAAG ATGGTTCTGA ATGAAACCTA CAGAAACATC 
AAAGTGCTAC TGACATCAGA TAAAGCTGCA GCCAATTTTT CAGACCGTTC CCTACTGAAG 
AACCTGGGCC ACTGGCTGGG AATGATTACA CTAGCTAAAA ATAAACCCAT CTTGCACACT 
GACTTGGATG TAAAATCTTT GCTGCTCGAA GCTTACGTTA AAGGACAACA AGAATTGCTT 
TATGTGGTGC CCTTTGTTGC CAAAGTACTG GAATCCAGTG TTCGAAGTGT GGTGTTCAGG 
CCACCAAACC CCTGGACTAT GGCTATCATG AACGTCTTAG CCGAGTTACA TCAAGAACAT 
GACCTGAAGC TAAACTTGAA ATTTGAAATT GAGGTGCTCT GCAAGAACCT TGCGTTAGAC 
ATCAGTGAGC TAAAACCCGG GAACCTTCTG AAGGATAAAG ATCGATTAAA AAACTTAGAA 
GAACAGCTCT CTGCTCCAAA GAAAGATGTA AAACAACCAG AAGAACTTCC AGCCATCACA 
ACAACTACGG CCTCTACAAC ACCAGCCACC AGTACGACCT GTACGGCGAC AGTTCCTCCT 
CAGCCCCAGT ACAGCTACCA CGACATCAAC GTGTACTCGC TCGGAGGGCT GGCCCCACAC 
ATTACTCTAA ATCCCACGAT TCCGTTGTTC CAGGCCCATC CACAGTTAAA GCAGTGTGTG 
CGCCAAGCAA TCGAACGTGC TGTACAAGAG CTTGTCCATC CGGTGGTGGA TAGGTCCATT 
AAGATTGCCA TGACTACTTG TGAACAAATA GTCAGGAAGG ATTTTGCACT GGATTCAGAG 
GAGTCACGCA TGCGAATAGC AGCACATCAC ATGATGAGGA ATCTGACAGC CGGAATGGCT 
ATGATAACTT GCAGGGAACC TTTGCTGATG AGTATAGCTA CCAACCTGAA AAACAGCTTC 
GCCACCGCAC TGCGGGCTGC GTCTCCTCAG CAAAGGGAAA TGATGGAGCA GGCAGCTGCG 
CAGTTAGCTC AGGACAATTG TGAATTAGCC TGCTGCTTCA TTCAGAAGAC TGCCGTAGAA 
AAAGCAGGCC CCGAAATGGA CAAGAGGCTA GCAACTGAGT TTGAGCTGCG CAAACACGCT 
AGGCAGGAGG GCCGTAGGTA CTGTGATCCC GTTGTATTAA CCTATCAGGC TGAGCGGATG 
CCAGAACAGA TCAGGCTGAA GGTAGGAGGT GTGGACCCCA AACAGCTGGC TGTTTATGAG 
GAGTTTGCAC GCAATGTTCC TGGCTTCTTG CCTACTAATG ATTTAACCCA GCCCACGGGA 
TTCTTAGCGC AGCCTATGAA ACAAGCTTGG GCAACAGACG ATGTAGCCCA GATTTATGAC 
AAGTGTATTA CAGAACTGGA ACAGCACCTG CATGCCATTC CACCTGCATT GGCCATGAAC 
CCTCAAGCTC AGGCTCTTCG CAGCCTTTTG GAAGCTGTGG TGCTGTCTCG CAACTCTCGT 
GATGCCATAG CGGCACTTGG GTTGCTTCAG AAGGCAGTAG AGGGTTTACT GGATGCCACA 
AGCGGTGCAG ATGCCGACCT ATTGCTACGT TACCGAGAGT GTCATCTACT TGTACTGAAA 
GCGCTGCAGG ATGGTCGGGC CTATGGTTCA CCGTGGTGTA ATAAACAGAT CACGAGGTGC 
CTGATCGAGT GTCGGGATGA ATATAAGTAT AACGTTGAAG CAGTGGAACT GCTGATTCGG 
AACCATCTAG TTAACATGCA GCAGTATGAC CTGCACCTGG CTCAGTCAAT GGAGAATGGT 
TTGAACTACA TGGCAGTGGC ATTTGCTATG CAGTTGGTGA AGATCCTGTT GGTGGATGAA 
AGGACTGTAG CCCATGTAAC AGAGGCAGAT TTATTTCACA CCATTGAAAC TCTGATGAGG 
ATTAATGCTC ACTCCAGAGG AAATGCCCCC GAAGGATTAC CCCAGCTGAT GGAAGTGGTG 
CGTGCCAACT ATGAAGCAAT GATTGATCGT GCTCATGGAG GTCCTAACTT CATGATGCAC 
TCTGGGATCT CTCAAGCCTC AGAATATGAT GACCCTCCAG GCCTAAGGGA GAAGGCAGAG 
TATCTTCTGA GGGAATGGGT GAATCTCTAC CATTCAGCCG CAGCTGGTCG CGACAGTACC 
AAAGCTTTCT CTGCGTTTGT TGGACAGGTA GAGCTTTTGG AAAGAAAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCAT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075146.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930979.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X11, mRNA.

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CloneID	OOc112965
Clone ID Related Accession (Same CDS sequence)	XM_029075146.1
Accession Version	XM_029075146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGGTGG TCTCGGTACA GGCAGCCTGA CCGGTATAGG AACTGCCGCT 
CTTGGGCTCC CCGCAGTGAA TAATGATCCG TTTGTGCAGA GGAAACTGGG CACATCTGGA 
CTGAACCAGC CTACATTCCA GCAGAGTAAG ATGAAACCTT CGGACTTGTC TCAGGTGTGG 
CCAGAGGCAA ATCAGCACTT TAGCAAAGAG ATAGATGATG AAGCAAACAG CTATTTTCAG 
CGTATTTATA ACCATCCACC ACACCCAACC ATGTCTGTTG ATGAGGTTTT AGAAATGCTA 
CAGAGGTTTA AAGACTCCAA CATAAAACGG GAACGAGAAG TGTTTAACTG TATGTTGAGG 
AACTTGTTTG AAGAGTATCG GTTTTTCCCC CAGTACCCAG ATAAAGAGCT GCATATAACA 
GCCTGCCTCT TTGGTGGGAT AATAGAAAAA GGACTGGTCA CTTACATGGC TCTGGGTCTT 
GCCCTGCGCT ATGTTCTTGA AGCCTTACGC AAGCCGTTTG CATCCAAAAT GTATTATTTT 
GGAATCGCTG CACTAGATAG GTTTAAAAAT AGATTGAAGG ACTATCCCCA GTATTGTCAG 
CACTTGGCTT CTATTAGTCA CTTTATACAG TTTCCTCATC ATCTACAGGA GTACATTGAA 
TACGGACAGC AATCTAGAGA CCCTCCTGTA AAAATGCAGG GTTCCATCAC CACCCCTGGA 
AGTATTGCAC TTGCCCAGGC TCAGGCCCAG GCCCAGGTTC CAGCAAAAGC TCCTCTTGCT 
GGTCAAGTCA GCACTATAGT AACCACCTCT ACCACCACCA CTGCTAAAAC CATCACGATC 
ACCCGACCAA CAGGAGTCAG CTTCAAAAAG GATGTGCCAC CTTCTATTAA TACTACCAAC 
ATAGATACAC TGCTAGTGGC GACCGATCAA ACTGAGAGAA TTGTGGAACC ACCAGAAAAT 
GTGCAAGAGA AAATTGCTTT CATCTTCAAC AATCTCTCTC AATCAAACAT GACACAGAAG 
GTTGAGGAGC TGAAAGAAAC GGTAAAAGAG GAATTTATGC CTTGGGTTTC ACAATACCTG 
GTAATGAAGA GAGTCAGTAT TGAGCCAAAC TTCCACAGCT TGTATTCAAA TTTCCTCGAT 
ACTCTTAAGA ACCCTGAATT TAACAAGATG GTTCTGAATG AAACCTACAG AAACATCAAA 
GTGCTACTGA CATCAGATAA AGCTGCAGCC AATTTTTCAG ACCGTTCCCT ACTGAAGAAC 
CTGGGCCACT GGCTGGGAAT GATTACACTA GCTAAAAATA AACCCATCTT GCACACTGAC 
TTGGATGTAA AATCTTTGCT GCTCGAAGCT TACGTTAAAG GACAACAAGA ATTGCTTTAT 
GTGGTGCCCT TTGTTGCCAA AGTACTGGAA TCCAGTGTTC GAAGTGTGGT GTTCAGGCCA 
CCAAACCCCT GGACTATGGC TATCATGAAC GTCTTAGCCG AGTTACATCA AGAACATGAC 
CTGAAGCTAA ACTTGAAATT TGAAATTGAG GTGCTCTGCA AGAACCTTGC GTTAGACATC 
AGTGAGCTAA AACCCGGGAA CCTTCTGAAG GATAAAGATC GATTAAAAAA CTTAGAAGAA 
CAGCTCTCTG CTCCAAAGAA AGATGTAAAA CAACCAGAAG AACTTCCAGC CATCACAACA 
ACTACGGCCT CTACAACACC AGCCACCAGT ACGACCTGTA CGGCGACAGT TCCTCCTCAG 
CCCCAGTACA GCTACCACGA CATCAACGTG TACTCGCTCG GAGGGCTGGC CCCACACATT 
ACTCTAAATC CCACGATTCC GTTGTTCCAG GCCCATCCAC AGTTAAAGCA GTGTGTGCGC 
CAAGCAATCG AACGTGCTGT ACAAGAGCTT GTCCATCCGG TGGTGGATAG GTCCATTAAG 
ATTGCCATGA CTACTTGTGA ACAAATAGTC AGGAAGGATT TTGCACTGGA TTCAGAGGAG 
TCACGCATGC GAATAGCAGC ACATCACATG ATGAGGAATC TGACAGCCGG AATGGCTATG 
ATAACTTGCA GGGAACCTTT GCTGATGAGT ATAGCTACCA ACCTGAAAAA CAGCTTCGCC 
ACCGCACTGC GGGCTGCGTC TCCTCAGCAA AGGGAAATGA TGGAGCAGGC AGCTGCGCAG 
TTAGCTCAGG ACAATTGTGA ATTAGCCTGC TGCTTCATTC AGAAGACTGC CGTAGAAAAA 
GCAGGCCCCG AAATGGACAA GAGGCTAGCA ACTGAGTTTG AGCTGCGCAA ACACGCTAGG 
CAGGAGGGCC GTAGGTACTG TGATCCCGTT GTATTAACCT ATCAGGCTGA GCGGATGCCA 
GAACAGATCA GGCTGAAGGT AGGAGGTGTG GACCCCAAAC AGCTGGCTGT TTATGAGGAG 
TTTGCACGCA ATGTTCCTGG CTTCTTGCCT ACTAATGATT TAACCCAGCC CACGGGATTC 
TTAGCGCAGC CTATGAAACA AGCTTGGGCA ACAGACGATG TAGCCCAGAT TTATGACAAG 
TGTATTACAG AACTGGAACA GCACCTGCAT GCCATTCCAC CTGCATTGGC CATGAACCCT 
CAAGCTCAGG CTCTTCGCAG CCTTTTGGAA GCTGTGGTGC TGTCTCGCAA CTCTCGTGAT 
GCCATAGCGG CACTTGGGTT GCTTCAGAAG GCAGTAGAGG GTTTACTGGA TGCCACAAGC 
GGTGCAGATG CCGACCTATT GCTACGTTAC CGAGAGTGTC ATCTACTTGT ACTGAAAGCG 
CTGCAGGATG GTCGGGCCTA TGGTTCACCG TGGTGTAATA AACAGATCAC GAGGTGCCTG 
ATCGAGTGTC GGGATGAATA TAAGTATAAC GTTGAAGCAG TGGAACTGCT GATTCGGAAC 
CATCTAGTTA ACATGCAGCA GTATGACCTG CACCTGGCTC AGTCAATGGA GAATGGTTTG 
AACTACATGG CAGTGGCATT TGCTATGCAG TTGGTGAAGA TCCTGTTGGT GGATGAAAGG 
ACTGTAGCCC ATGTAACAGA GGCAGATTTA TTTCACACCA TTGAAACTCT GATGAGGATT 
AATGCTCACT CCAGAGGAAA TGCCCCCGAA GGATTACCCC AGCTGATGGA AGTGGTGCGT 
GCCAACTATG AAGCAATGAT TGATCGTGCT CATGGAGGTC CTAACTTCAT GATGCACTCT 
GGGATCTCTC AAGCCTCAGA ATATGATGAC CCTCCAGGCC TAAGGGAGAA GGCAGAGTAT 
CTTCTGAGGG AATGGGTGAA TCTCTACCAT TCAGCCGCAG CTGGTCGCGA CAGTACCAAA 
GCTTTCTCTG CGTTTGTTGG ACAGGTAGAG CTTTTGGAAA GAAAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCATACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930979.1
CDS	224..7213
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075146.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075146.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGGTGG TCTCGGTACA GGCAGCCTGA CCGGTATAGG AACTGCCGCT 
CTTGGGCTCC CCGCAGTGAA TAATGATCCG TTTGTGCAGA GGAAACTGGG CACATCTGGA 
CTGAACCAGC CTACATTCCA GCAGAGTAAG ATGAAACCTT CGGACTTGTC TCAGGTGTGG 
CCAGAGGCAA ATCAGCACTT TAGCAAAGAG ATAGATGATG AAGCAAACAG CTATTTTCAG 
CGTATTTATA ACCATCCACC ACACCCAACC ATGTCTGTTG ATGAGGTTTT AGAAATGCTA 
CAGAGGTTTA AAGACTCCAA CATAAAACGG GAACGAGAAG TGTTTAACTG TATGTTGAGG 
AACTTGTTTG AAGAGTATCG GTTTTTCCCC CAGTACCCAG ATAAAGAGCT GCATATAACA 
GCCTGCCTCT TTGGTGGGAT AATAGAAAAA GGACTGGTCA CTTACATGGC TCTGGGTCTT 
GCCCTGCGCT ATGTTCTTGA AGCCTTACGC AAGCCGTTTG CATCCAAAAT GTATTATTTT 
GGAATCGCTG CACTAGATAG GTTTAAAAAT AGATTGAAGG ACTATCCCCA GTATTGTCAG 
CACTTGGCTT CTATTAGTCA CTTTATACAG TTTCCTCATC ATCTACAGGA GTACATTGAA 
TACGGACAGC AATCTAGAGA CCCTCCTGTA AAAATGCAGG GTTCCATCAC CACCCCTGGA 
AGTATTGCAC TTGCCCAGGC TCAGGCCCAG GCCCAGGTTC CAGCAAAAGC TCCTCTTGCT 
GGTCAAGTCA GCACTATAGT AACCACCTCT ACCACCACCA CTGCTAAAAC CATCACGATC 
ACCCGACCAA CAGGAGTCAG CTTCAAAAAG GATGTGCCAC CTTCTATTAA TACTACCAAC 
ATAGATACAC TGCTAGTGGC GACCGATCAA ACTGAGAGAA TTGTGGAACC ACCAGAAAAT 
GTGCAAGAGA AAATTGCTTT CATCTTCAAC AATCTCTCTC AATCAAACAT GACACAGAAG 
GTTGAGGAGC TGAAAGAAAC GGTAAAAGAG GAATTTATGC CTTGGGTTTC ACAATACCTG 
GTAATGAAGA GAGTCAGTAT TGAGCCAAAC TTCCACAGCT TGTATTCAAA TTTCCTCGAT 
ACTCTTAAGA ACCCTGAATT TAACAAGATG GTTCTGAATG AAACCTACAG AAACATCAAA 
GTGCTACTGA CATCAGATAA AGCTGCAGCC AATTTTTCAG ACCGTTCCCT ACTGAAGAAC 
CTGGGCCACT GGCTGGGAAT GATTACACTA GCTAAAAATA AACCCATCTT GCACACTGAC 
TTGGATGTAA AATCTTTGCT GCTCGAAGCT TACGTTAAAG GACAACAAGA ATTGCTTTAT 
GTGGTGCCCT TTGTTGCCAA AGTACTGGAA TCCAGTGTTC GAAGTGTGGT GTTCAGGCCA 
CCAAACCCCT GGACTATGGC TATCATGAAC GTCTTAGCCG AGTTACATCA AGAACATGAC 
CTGAAGCTAA ACTTGAAATT TGAAATTGAG GTGCTCTGCA AGAACCTTGC GTTAGACATC 
AGTGAGCTAA AACCCGGGAA CCTTCTGAAG GATAAAGATC GATTAAAAAA CTTAGAAGAA 
CAGCTCTCTG CTCCAAAGAA AGATGTAAAA CAACCAGAAG AACTTCCAGC CATCACAACA 
ACTACGGCCT CTACAACACC AGCCACCAGT ACGACCTGTA CGGCGACAGT TCCTCCTCAG 
CCCCAGTACA GCTACCACGA CATCAACGTG TACTCGCTCG GAGGGCTGGC CCCACACATT 
ACTCTAAATC CCACGATTCC GTTGTTCCAG GCCCATCCAC AGTTAAAGCA GTGTGTGCGC 
CAAGCAATCG AACGTGCTGT ACAAGAGCTT GTCCATCCGG TGGTGGATAG GTCCATTAAG 
ATTGCCATGA CTACTTGTGA ACAAATAGTC AGGAAGGATT TTGCACTGGA TTCAGAGGAG 
TCACGCATGC GAATAGCAGC ACATCACATG ATGAGGAATC TGACAGCCGG AATGGCTATG 
ATAACTTGCA GGGAACCTTT GCTGATGAGT ATAGCTACCA ACCTGAAAAA CAGCTTCGCC 
ACCGCACTGC GGGCTGCGTC TCCTCAGCAA AGGGAAATGA TGGAGCAGGC AGCTGCGCAG 
TTAGCTCAGG ACAATTGTGA ATTAGCCTGC TGCTTCATTC AGAAGACTGC CGTAGAAAAA 
GCAGGCCCCG AAATGGACAA GAGGCTAGCA ACTGAGTTTG AGCTGCGCAA ACACGCTAGG 
CAGGAGGGCC GTAGGTACTG TGATCCCGTT GTATTAACCT ATCAGGCTGA GCGGATGCCA 
GAACAGATCA GGCTGAAGGT AGGAGGTGTG GACCCCAAAC AGCTGGCTGT TTATGAGGAG 
TTTGCACGCA ATGTTCCTGG CTTCTTGCCT ACTAATGATT TAACCCAGCC CACGGGATTC 
TTAGCGCAGC CTATGAAACA AGCTTGGGCA ACAGACGATG TAGCCCAGAT TTATGACAAG 
TGTATTACAG AACTGGAACA GCACCTGCAT GCCATTCCAC CTGCATTGGC CATGAACCCT 
CAAGCTCAGG CTCTTCGCAG CCTTTTGGAA GCTGTGGTGC TGTCTCGCAA CTCTCGTGAT 
GCCATAGCGG CACTTGGGTT GCTTCAGAAG GCAGTAGAGG GTTTACTGGA TGCCACAAGC 
GGTGCAGATG CCGACCTATT GCTACGTTAC CGAGAGTGTC ATCTACTTGT ACTGAAAGCG 
CTGCAGGATG GTCGGGCCTA TGGTTCACCG TGGTGTAATA AACAGATCAC GAGGTGCCTG 
ATCGAGTGTC GGGATGAATA TAAGTATAAC GTTGAAGCAG TGGAACTGCT GATTCGGAAC 
CATCTAGTTA ACATGCAGCA GTATGACCTG CACCTGGCTC AGTCAATGGA GAATGGTTTG 
AACTACATGG CAGTGGCATT TGCTATGCAG TTGGTGAAGA TCCTGTTGGT GGATGAAAGG 
ACTGTAGCCC ATGTAACAGA GGCAGATTTA TTTCACACCA TTGAAACTCT GATGAGGATT 
AATGCTCACT CCAGAGGAAA TGCCCCCGAA GGATTACCCC AGCTGATGGA AGTGGTGCGT 
GCCAACTATG AAGCAATGAT TGATCGTGCT CATGGAGGTC CTAACTTCAT GATGCACTCT 
GGGATCTCTC AAGCCTCAGA ATATGATGAC CCTCCAGGCC TAAGGGAGAA GGCAGAGTAT 
CTTCTGAGGG AATGGGTGAA TCTCTACCAT TCAGCCGCAG CTGGTCGCGA CAGTACCAAA 
GCTTTCTCTG CGTTTGTTGG ACAGGTAGAG CTTTTGGAAA GAAAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCATACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_029075147.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028930980.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X12, mRNA.

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CloneID	OOc112966
Clone ID Related Accession (Same CDS sequence)	XM_029075147.1
Accession Version	XM_029075147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041738.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCATACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028930980.1
CDS	224..7333
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_029075147.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029075147.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCATACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671971.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670161.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

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CloneID	OOc13651
Clone ID Related Accession (Same CDS sequence)	XM_007671971.1
Accession Version	XM_007671971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCGTACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670161.1
CDS	195..7343
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671971.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671971.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCTTGAAGAC CGACGACCTC 
ATAACAAGGT TCTTTCGCCT GTGCACAGAA ATGTGTGTTG AGATCAGCTA CCGAGCTCAG 
GCCGAGCAGC AACACAATCC CGCAGCCAAC CCTACCATGA TCCGAGCCAA ATGCTATCAT 
AACCTGGATG CTTTTGTGCG ACTTATTGCC CTCCTGGTCA AACATTCGGG GGAGGCCACC 
AATACGGTCA CCAAGATAAA CCTGCTGAAT AAGGTTCTTG GGATCGTGGT GGGCGTCCTC 
CTGCAGGATC ACGATGTTCG GCAAAACGAT TTTCAGCAGC TTCCGTACCA CCGTATTTTC 
ATCATGTTGC TTTTGGAACT CAATGCCCCC GAGCATGTCT TGGAGACCAT TAATTTTCAG 
ACGCTCACAG CTTTCTGCAA TACATTCCAC ATCTTGAGGC CTACCAAAGC TCCCGGCTTT 
GTGTATGCCT GGCTTGAGCT GATCTCCCAC CGGATATTTA TCGCACGGAT GCTGGCACAT 
ACACCACAGC AGAAGGGCTG GCCCATGTAT GCCCAACTAC TGATTGATTT GTTCAAATAT 
TTGGCTCCCT TCCTTAGAAA TGTGGAACTT ACCAAACCTA TGCAAATCCT GTACAAGGGT 
ACTTTACGAG TGCTCCTGGT CTTATTGCAT GACTTTCCGG AATTCCTTTG TGATTACCAT 
TATGGGTTCT GTGATGTCAT CCCACCCAAT TGCATCCAGC TAAGAAATTT GATCCTGAGT 
GCCTTTCCAC GCAACATGAG GCTCCCAGAC CCATTCACTC CCAACTTAAA GGTGGATATG 
TTGAGTGAAA TTAACATCGC TCCTAGAATC CTCACCAATT TCACAGGAGT GATGCCACCT 
CAGTTCAAAA AAGATCTGGA TTCCTATCTC AAAACCCGCT CACCAGTCAC GTTCCTTTCG 
GACTTGCGCA GCAACCTGCA GGTATCGAAT GAACCCGGTA ATCGTTACAA CATCCAGTTG 
ATCAACGCCC TGGTGCTCTA CGTAGGTACT CAAGCCATCG CCCACATCCA CAATAAAGGT 
AGCACGCCAT CCATGAGCAC CATCACCCAC TCGGCTCACA TGGACATCTT CCAGAACCTG 
GCCGTGGACC TGGACACTGA AGGTCGGTAC CTGTTCCTGA ATGCCATCGC TAACCAGCTG 
CGGTACCCGA ATAGCCACAC TCATTACTTC AGTTGTACCA TGCTGTACCT GTTCGCAGAA 
GCCAACACTG AAGCCATCCA GGAACAGATC ACCAGGGTTC TCTTGGAACG GTTGATTGTA 
AATAGGCCAC ATCCTTGGGG TCTCCTCATT ACCTTCATCG AGCTGATTAA AAACCCAGCG 
TTTAAGTTCT GGAACCACGA GTTCGTTCAC TGTGCCCCAG AAATTGAAAA GTTGTTCCAG 
TCGGTTGCAC AATGCTGCAT GGGGCAGAAG CAGGCTCAGC AAGTGATGGA AGGGACCGGT 
GCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671972.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670162.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

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CloneID	OOc13652
Clone ID Related Accession (Same CDS sequence)	XM_007671972.1
Accession Version	XM_007671972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CGTACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670162.1
CDS	195..7340
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671972.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671972.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CGTACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671973.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670163.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

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CloneID	OOc13653
Clone ID Related Accession (Same CDS sequence)	XM_007671973.1
Accession Version	XM_007671973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGGT AGAGCTTTTG 
GAAAGAAAGA TGCACCAGCA AGGAATCTTG AAGACCGACG ACCTCATAAC AAGGTTCTTT 
CGCCTGTGCA CAGAAATGTG TGTTGAGATC AGCTACCGAG CTCAGGCCGA GCAGCAACAC 
AATCCCGCAG CCAACCCTAC CATGATCCGA GCCAAATGCT ATCATAACCT GGATGCTTTT 
GTGCGACTTA TTGCCCTCCT GGTCAAACAT TCGGGGGAGG CCACCAATAC GGTCACCAAG 
ATAAACCTGC TGAATAAGGT TCTTGGGATC GTGGTGGGCG TCCTCCTGCA GGATCACGAT 
GTTCGGCAAA ACGATTTTCA GCAGCTTCCG TACCACCGTA TTTTCATCAT GTTGCTTTTG 
GAACTCAATG CCCCCGAGCA TGTCTTGGAG ACCATTAATT TTCAGACGCT CACAGCTTTC 
TGCAATACAT TCCACATCTT GAGGCCTACC AAAGCTCCCG GCTTTGTGTA TGCCTGGCTT 
GAGCTGATCT CCCACCGGAT ATTTATCGCA CGGATGCTGG CACATACACC ACAGCAGAAG 
GGCTGGCCCA TGTATGCCCA ACTACTGATT GATTTGTTCA AATATTTGGC TCCCTTCCTT 
AGAAATGTGG AACTTACCAA ACCTATGCAA ATCCTGTACA AGGGTACTTT ACGAGTGCTC 
CTGGTCTTAT TGCATGACTT TCCGGAATTC CTTTGTGATT ACCATTATGG GTTCTGTGAT 
GTCATCCCAC CCAATTGCAT CCAGCTAAGA AATTTGATCC TGAGTGCCTT TCCACGCAAC 
ATGAGGCTCC CAGACCCATT CACTCCCAAC TTAAAGGTGG ATATGTTGAG TGAAATTAAC 
ATCGCTCCTA GAATCCTCAC CAATTTCACA GGAGTGATGC CACCTCAGTT CAAAAAAGAT 
CTGGATTCCT ATCTCAAAAC CCGCTCACCA GTCACGTTCC TTTCGGACTT GCGCAGCAAC 
CTGCAGGTAT CGAATGAACC CGGTAATCGT TACAACATCC AGTTGATCAA CGCCCTGGTG 
CTCTACGTAG GTACTCAAGC CATCGCCCAC ATCCACAATA AAGGTAGCAC GCCATCCATG 
AGCACCATCA CCCACTCGGC TCACATGGAC ATCTTCCAGA ACCTGGCCGT GGACCTGGAC 
ACTGAAGGTC GGTACCTGTT CCTGAATGCC ATCGCTAACC AGCTGCGGTA CCCGAATAGC 
CACACTCATT ACTTCAGTTG TACCATGCTG TACCTGTTCG CAGAAGCCAA CACTGAAGCC 
ATCCAGGAAC AGATCACCAG GGTTCTCTTG GAACGGTTGA TTGTAAATAG GCCACATCCT 
TGGGGTCTCC TCATTACCTT CATCGAGCTG ATTAAAAACC CAGCGTTTAA GTTCTGGAAC 
CACGAGTTCG TTCACTGTGC CCCAGAAATT GAAAAGTTGT TCCAGTCGGT TGCACAATGC 
TGCATGGGGC AGAAGCAGGC TCAGCAAGTG ATGGAAGGGA CCGGTGCCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670163.1
CDS	195..7328
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671973.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671973.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGGT AGAGCTTTTG 
GAAAGAAAGA TGCACCAGCA AGGAATCTTG AAGACCGACG ACCTCATAAC AAGGTTCTTT 
CGCCTGTGCA CAGAAATGTG TGTTGAGATC AGCTACCGAG CTCAGGCCGA GCAGCAACAC 
AATCCCGCAG CCAACCCTAC CATGATCCGA GCCAAATGCT ATCATAACCT GGATGCTTTT 
GTGCGACTTA TTGCCCTCCT GGTCAAACAT TCGGGGGAGG CCACCAATAC GGTCACCAAG 
ATAAACCTGC TGAATAAGGT TCTTGGGATC GTGGTGGGCG TCCTCCTGCA GGATCACGAT 
GTTCGGCAAA ACGATTTTCA GCAGCTTCCG TACCACCGTA TTTTCATCAT GTTGCTTTTG 
GAACTCAATG CCCCCGAGCA TGTCTTGGAG ACCATTAATT TTCAGACGCT CACAGCTTTC 
TGCAATACAT TCCACATCTT GAGGCCTACC AAAGCTCCCG GCTTTGTGTA TGCCTGGCTT 
GAGCTGATCT CCCACCGGAT ATTTATCGCA CGGATGCTGG CACATACACC ACAGCAGAAG 
GGCTGGCCCA TGTATGCCCA ACTACTGATT GATTTGTTCA AATATTTGGC TCCCTTCCTT 
AGAAATGTGG AACTTACCAA ACCTATGCAA ATCCTGTACA AGGGTACTTT ACGAGTGCTC 
CTGGTCTTAT TGCATGACTT TCCGGAATTC CTTTGTGATT ACCATTATGG GTTCTGTGAT 
GTCATCCCAC CCAATTGCAT CCAGCTAAGA AATTTGATCC TGAGTGCCTT TCCACGCAAC 
ATGAGGCTCC CAGACCCATT CACTCCCAAC TTAAAGGTGG ATATGTTGAG TGAAATTAAC 
ATCGCTCCTA GAATCCTCAC CAATTTCACA GGAGTGATGC CACCTCAGTT CAAAAAAGAT 
CTGGATTCCT ATCTCAAAAC CCGCTCACCA GTCACGTTCC TTTCGGACTT GCGCAGCAAC 
CTGCAGGTAT CGAATGAACC CGGTAATCGT TACAACATCC AGTTGATCAA CGCCCTGGTG 
CTCTACGTAG GTACTCAAGC CATCGCCCAC ATCCACAATA AAGGTAGCAC GCCATCCATG 
AGCACCATCA CCCACTCGGC TCACATGGAC ATCTTCCAGA ACCTGGCCGT GGACCTGGAC 
ACTGAAGGTC GGTACCTGTT CCTGAATGCC ATCGCTAACC AGCTGCGGTA CCCGAATAGC 
CACACTCATT ACTTCAGTTG TACCATGCTG TACCTGTTCG CAGAAGCCAA CACTGAAGCC 
ATCCAGGAAC AGATCACCAG GGTTCTCTTG GAACGGTTGA TTGTAAATAG GCCACATCCT 
TGGGGTCTCC TCATTACCTT CATCGAGCTG ATTAAAAACC CAGCGTTTAA GTTCTGGAAC 
CACGAGTTCG TTCACTGTGC CCCAGAAATT GAAAAGTTGT TCCAGTCGGT TGCACAATGC 
TGCATGGGGC AGAAGCAGGC TCAGCAAGTG ATGGAAGGGA CCGGTGCCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671974.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670164.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

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CloneID	OOc13654
Clone ID Related Accession (Same CDS sequence)	XM_007671974.1
Accession Version	XM_007671974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA 
AGAAAGATGC ACCAGCAAGG AATCTTGAAG ACCGACGACC TCATAACAAG GTTCTTTCGC 
CTGTGCACAG AAATGTGTGT TGAGATCAGC TACCGAGCTC AGGCCGAGCA GCAACACAAT 
CCCGCAGCCA ACCCTACCAT GATCCGAGCC AAATGCTATC ATAACCTGGA TGCTTTTGTG 
CGACTTATTG CCCTCCTGGT CAAACATTCG GGGGAGGCCA CCAATACGGT CACCAAGATA 
AACCTGCTGA ATAAGGTTCT TGGGATCGTG GTGGGCGTCC TCCTGCAGGA TCACGATGTT 
CGGCAAAACG ATTTTCAGCA GCTTCCGTAC CACCGTATTT TCATCATGTT GCTTTTGGAA 
CTCAATGCCC CCGAGCATGT CTTGGAGACC ATTAATTTTC AGACGCTCAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCCGGCT TTGTGTATGC CTGGCTTGAG 
CTGATCTCCC ACCGGATATT TATCGCACGG ATGCTGGCAC ATACACCACA GCAGAAGGGC 
TGGCCCATGT ATGCCCAACT ACTGATTGAT TTGTTCAAAT ATTTGGCTCC CTTCCTTAGA 
AATGTGGAAC TTACCAAACC TATGCAAATC CTGTACAAGG GTACTTTACG AGTGCTCCTG 
GTCTTATTGC ATGACTTTCC GGAATTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTC 
ATCCCACCCA ATTGCATCCA GCTAAGAAAT TTGATCCTGA GTGCCTTTCC ACGCAACATG 
AGGCTCCCAG ACCCATTCAC TCCCAACTTA AAGGTGGATA TGTTGAGTGA AATTAACATC 
GCTCCTAGAA TCCTCACCAA TTTCACAGGA GTGATGCCAC CTCAGTTCAA AAAAGATCTG 
GATTCCTATC TCAAAACCCG CTCACCAGTC ACGTTCCTTT CGGACTTGCG CAGCAACCTG 
CAGGTATCGA ATGAACCCGG TAATCGTTAC AACATCCAGT TGATCAACGC CCTGGTGCTC 
TACGTAGGTA CTCAAGCCAT CGCCCACATC CACAATAAAG GTAGCACGCC ATCCATGAGC 
ACCATCACCC ACTCGGCTCA CATGGACATC TTCCAGAACC TGGCCGTGGA CCTGGACACT 
GAAGGTCGGT ACCTGTTCCT GAATGCCATC GCTAACCAGC TGCGGTACCC GAATAGCCAC 
ACTCATTACT TCAGTTGTAC CATGCTGTAC CTGTTCGCAG AAGCCAACAC TGAAGCCATC 
CAGGAACAGA TCACCAGGGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTCCTCA TTACCTTCAT CGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAC 
GAGTTCGTTC ACTGTGCCCC AGAAATTGAA AAGTTGTTCC AGTCGGTTGC ACAATGCTGC 
ATGGGGCAGA AGCAGGCTCA GCAAGTGATG GAAGGGACCG GTGCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670164.1
CDS	195..7325
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671974.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671974.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA 
AGAAAGATGC ACCAGCAAGG AATCTTGAAG ACCGACGACC TCATAACAAG GTTCTTTCGC 
CTGTGCACAG AAATGTGTGT TGAGATCAGC TACCGAGCTC AGGCCGAGCA GCAACACAAT 
CCCGCAGCCA ACCCTACCAT GATCCGAGCC AAATGCTATC ATAACCTGGA TGCTTTTGTG 
CGACTTATTG CCCTCCTGGT CAAACATTCG GGGGAGGCCA CCAATACGGT CACCAAGATA 
AACCTGCTGA ATAAGGTTCT TGGGATCGTG GTGGGCGTCC TCCTGCAGGA TCACGATGTT 
CGGCAAAACG ATTTTCAGCA GCTTCCGTAC CACCGTATTT TCATCATGTT GCTTTTGGAA 
CTCAATGCCC CCGAGCATGT CTTGGAGACC ATTAATTTTC AGACGCTCAC AGCTTTCTGC 
AATACATTCC ACATCTTGAG GCCTACCAAA GCTCCCGGCT TTGTGTATGC CTGGCTTGAG 
CTGATCTCCC ACCGGATATT TATCGCACGG ATGCTGGCAC ATACACCACA GCAGAAGGGC 
TGGCCCATGT ATGCCCAACT ACTGATTGAT TTGTTCAAAT ATTTGGCTCC CTTCCTTAGA 
AATGTGGAAC TTACCAAACC TATGCAAATC CTGTACAAGG GTACTTTACG AGTGCTCCTG 
GTCTTATTGC ATGACTTTCC GGAATTCCTT TGTGATTACC ATTATGGGTT CTGTGATGTC 
ATCCCACCCA ATTGCATCCA GCTAAGAAAT TTGATCCTGA GTGCCTTTCC ACGCAACATG 
AGGCTCCCAG ACCCATTCAC TCCCAACTTA AAGGTGGATA TGTTGAGTGA AATTAACATC 
GCTCCTAGAA TCCTCACCAA TTTCACAGGA GTGATGCCAC CTCAGTTCAA AAAAGATCTG 
GATTCCTATC TCAAAACCCG CTCACCAGTC ACGTTCCTTT CGGACTTGCG CAGCAACCTG 
CAGGTATCGA ATGAACCCGG TAATCGTTAC AACATCCAGT TGATCAACGC CCTGGTGCTC 
TACGTAGGTA CTCAAGCCAT CGCCCACATC CACAATAAAG GTAGCACGCC ATCCATGAGC 
ACCATCACCC ACTCGGCTCA CATGGACATC TTCCAGAACC TGGCCGTGGA CCTGGACACT 
GAAGGTCGGT ACCTGTTCCT GAATGCCATC GCTAACCAGC TGCGGTACCC GAATAGCCAC 
ACTCATTACT TCAGTTGTAC CATGCTGTAC CTGTTCGCAG AAGCCAACAC TGAAGCCATC 
CAGGAACAGA TCACCAGGGT TCTCTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG 
GGTCTCCTCA TTACCTTCAT CGAGCTGATT AAAAACCCAG CGTTTAAGTT CTGGAACCAC 
GAGTTCGTTC ACTGTGCCCC AGAAATTGAA AAGTTGTTCC AGTCGGTTGC ACAATGCTGC 
ATGGGGCAGA AGCAGGCTCA GCAAGTGATG GAAGGGACCG GTGCCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_003431273.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_003431321.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

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CloneID	OOc13655
Clone ID Related Accession (Same CDS sequence)	XM_003431273.2
Accession Version	XM_003431273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 29, 2014 this sequence version replaced XM_003431273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGATGCACC AGCAAGGAAT CTTGAAGACC GACGACCTCA TAACAAGGTT CTTTCGCCTG 
TGCACAGAAA TGTGTGTTGA GATCAGCTAC CGAGCTCAGG CCGAGCAGCA ACACAATCCC 
GCAGCCAACC CTACCATGAT CCGAGCCAAA TGCTATCATA ACCTGGATGC TTTTGTGCGA 
CTTATTGCCC TCCTGGTCAA ACATTCGGGG GAGGCCACCA ATACGGTCAC CAAGATAAAC 
CTGCTGAATA AGGTTCTTGG GATCGTGGTG GGCGTCCTCC TGCAGGATCA CGATGTTCGG 
CAAAACGATT TTCAGCAGCT TCCGTACCAC CGTATTTTCA TCATGTTGCT TTTGGAACTC 
AATGCCCCCG AGCATGTCTT GGAGACCATT AATTTTCAGA CGCTCACAGC TTTCTGCAAT 
ACATTCCACA TCTTGAGGCC TACCAAAGCT CCCGGCTTTG TGTATGCCTG GCTTGAGCTG 
ATCTCCCACC GGATATTTAT CGCACGGATG CTGGCACATA CACCACAGCA GAAGGGCTGG 
CCCATGTATG CCCAACTACT GATTGATTTG TTCAAATATT TGGCTCCCTT CCTTAGAAAT 
GTGGAACTTA CCAAACCTAT GCAAATCCTG TACAAGGGTA CTTTACGAGT GCTCCTGGTC 
TTATTGCATG ACTTTCCGGA ATTCCTTTGT GATTACCATT ATGGGTTCTG TGATGTCATC 
CCACCCAATT GCATCCAGCT AAGAAATTTG ATCCTGAGTG CCTTTCCACG CAACATGAGG 
CTCCCAGACC CATTCACTCC CAACTTAAAG GTGGATATGT TGAGTGAAAT TAACATCGCT 
CCTAGAATCC TCACCAATTT CACAGGAGTG ATGCCACCTC AGTTCAAAAA AGATCTGGAT 
TCCTATCTCA AAACCCGCTC ACCAGTCACG TTCCTTTCGG ACTTGCGCAG CAACCTGCAG 
GTATCGAATG AACCCGGTAA TCGTTACAAC ATCCAGTTGA TCAACGCCCT GGTGCTCTAC 
GTAGGTACTC AAGCCATCGC CCACATCCAC AATAAAGGTA GCACGCCATC CATGAGCACC 
ATCACCCACT CGGCTCACAT GGACATCTTC CAGAACCTGG CCGTGGACCT GGACACTGAA 
GGTCGGTACC TGTTCCTGAA TGCCATCGCT AACCAGCTGC GGTACCCGAA TAGCCACACT 
CATTACTTCA GTTGTACCAT GCTGTACCTG TTCGCAGAAG CCAACACTGA AGCCATCCAG 
GAACAGATCA CCAGGGTTCT CTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT 
CTCCTCATTA CCTTCATCGA GCTGATTAAA AACCCAGCGT TTAAGTTCTG GAACCACGAG 
TTCGTTCACT GTGCCCCAGA AATTGAAAAG TTGTTCCAGT CGGTTGCACA ATGCTGCATG 
GGGCAGAAGC AGGCTCAGCA AGTGATGGAA GGGACCGGTG CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431321.1
CDS	195..7322
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_003431273.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431273.2

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT 
AGCAAAGAGA TAGATGATGA AGCAAACAGC TATTTTCAGC GTATTTATAA CCATCCACCA 
CACCCAACCA TGTCTGTTGA TGAGGTTTTA GAAATGCTAC AGAGGTTTAA AGACTCCAAC 
ATAAAACGGG AACGAGAAGT GTTTAACTGT ATGTTGAGGA ACTTGTTTGA AGAGTATCGG 
TTTTTCCCCC AGTACCCAGA TAAAGAGCTG CATATAACAG CCTGCCTCTT TGGTGGGATA 
ATAGAAAAAG GACTGGTCAC TTACATGGCT CTGGGTCTTG CCCTGCGCTA TGTTCTTGAA 
GCCTTACGCA AGCCGTTTGC ATCCAAAATG TATTATTTTG GAATCGCTGC ACTAGATAGG 
TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC TATTAGTCAC 
TTTATACAGT TTCCTCATCA TCTACAGGAG TACATTGAAT ACGGACAGCA ATCTAGAGAC 
CCTCCTGTAA AAATGCAGGG TTCCATCACC ACCCCTGGAA GTATTGCACT TGCCCAGGCT 
CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCTCTTGCTG GTCAAGTCAG CACTATAGTA 
ACCACCTCTA CCACCACCAC TGCTAAAACC ATCACGATCA CCCGACCAAC AGGAGTCAGC 
TTCAAAAAGG ATGTGCCACC TTCTATTAAT ACTACCAACA TAGATACACT GCTAGTGGCG 
ACCGATCAAA CTGAGAGAAT TGTGGAACCA CCAGAAAATG TGCAAGAGAA AATTGCTTTC 
ATCTTCAACA ATCTCTCTCA ATCAAACATG ACACAGAAGG TTGAGGAGCT GAAAGAAACG 
GTAAAAGAGG AATTTATGCC TTGGGTTTCA CAATACCTGG TAATGAAGAG AGTCAGTATT 
GAGCCAAACT TCCACAGCTT GTATTCAAAT TTCCTCGATA CTCTTAAGAA CCCTGAATTT 
AACAAGATGG TTCTGAATGA AACCTACAGA AACATCAAAG TGCTACTGAC ATCAGATAAA 
GCTGCAGCCA ATTTTTCAGA CCGTTCCCTA CTGAAGAACC TGGGCCACTG GCTGGGAATG 
ATTACACTAG CTAAAAATAA ACCCATCTTG CACACTGACT TGGATGTAAA ATCTTTGCTG 
CTCGAAGCTT ACGTTAAAGG ACAACAAGAA TTGCTTTATG TGGTGCCCTT TGTTGCCAAA 
GTACTGGAAT CCAGTGTTCG AAGTGTGGTG TTCAGGCCAC CAAACCCCTG GACTATGGCT 
ATCATGAACG TCTTAGCCGA GTTACATCAA GAACATGACC TGAAGCTAAA CTTGAAATTT 
GAAATTGAGG TGCTCTGCAA GAACCTTGCG TTAGACATCA GTGAGCTAAA ACCCGGGAAC 
CTTCTGAAGG ATAAAGATCG ATTAAAAAAC TTAGAAGAAC AGCTCTCTGC TCCAAAGAAA 
GATGTAAAAC AACCAGAAGA ACTTCCAGCC ATCACAACAA CTACGGCCTC TACAACACCA 
GCCACCAGTA CGACCTGTAC GGCGACAGTT CCTCCTCAGC CCCAGTACAG CTACCACGAC 
ATCAACGTGT ACTCGCTCGG AGGGCTGGCC CCACACATTA CTCTAAATCC CACGATTCCG 
TTGTTCCAGG CCCATCCACA GTTAAAGCAG TGTGTGCGCC AAGCAATCGA ACGTGCTGTA 
CAAGAGCTTG TCCATCCGGT GGTGGATAGG TCCATTAAGA TTGCCATGAC TACTTGTGAA 
CAAATAGTCA GGAAGGATTT TGCACTGGAT TCAGAGGAGT CACGCATGCG AATAGCAGCA 
CATCACATGA TGAGGAATCT GACAGCCGGA ATGGCTATGA TAACTTGCAG GGAACCTTTG 
CTGATGAGTA TAGCTACCAA CCTGAAAAAC AGCTTCGCCA CCGCACTGCG GGCTGCGTCT 
CCTCAGCAAA GGGAAATGAT GGAGCAGGCA GCTGCGCAGT TAGCTCAGGA CAATTGTGAA 
TTAGCCTGCT GCTTCATTCA GAAGACTGCC GTAGAAAAAG CAGGCCCCGA AATGGACAAG 
AGGCTAGCAA CTGAGTTTGA GCTGCGCAAA CACGCTAGGC AGGAGGGCCG TAGGTACTGT 
GATCCCGTTG TATTAACCTA TCAGGCTGAG CGGATGCCAG AACAGATCAG GCTGAAGGTA 
GGAGGTGTGG ACCCCAAACA GCTGGCTGTT TATGAGGAGT TTGCACGCAA TGTTCCTGGC 
TTCTTGCCTA CTAATGATTT AACCCAGCCC ACGGGATTCT TAGCGCAGCC TATGAAACAA 
GCTTGGGCAA CAGACGATGT AGCCCAGATT TATGACAAGT GTATTACAGA ACTGGAACAG 
CACCTGCATG CCATTCCACC TGCATTGGCC ATGAACCCTC AAGCTCAGGC TCTTCGCAGC 
CTTTTGGAAG CTGTGGTGCT GTCTCGCAAC TCTCGTGATG CCATAGCGGC ACTTGGGTTG 
CTTCAGAAGG CAGTAGAGGG TTTACTGGAT GCCACAAGCG GTGCAGATGC CGACCTATTG 
CTACGTTACC GAGAGTGTCA TCTACTTGTA CTGAAAGCGC TGCAGGATGG TCGGGCCTAT 
GGTTCACCGT GGTGTAATAA ACAGATCACG AGGTGCCTGA TCGAGTGTCG GGATGAATAT 
AAGTATAACG TTGAAGCAGT GGAACTGCTG ATTCGGAACC ATCTAGTTAA CATGCAGCAG 
TATGACCTGC ACCTGGCTCA GTCAATGGAG AATGGTTTGA ACTACATGGC AGTGGCATTT 
GCTATGCAGT TGGTGAAGAT CCTGTTGGTG GATGAAAGGA CTGTAGCCCA TGTAACAGAG 
GCAGATTTAT TTCACACCAT TGAAACTCTG ATGAGGATTA ATGCTCACTC CAGAGGAAAT 
GCCCCCGAAG GATTACCCCA GCTGATGGAA GTGGTGCGTG CCAACTATGA AGCAATGATT 
GATCGTGCTC ATGGAGGTCC TAACTTCATG ATGCACTCTG GGATCTCTCA AGCCTCAGAA 
TATGATGACC CTCCAGGCCT AAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT 
CTCTACCATT CAGCCGCAGC TGGTCGCGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA 
CAGATGCACC AGCAAGGAAT CTTGAAGACC GACGACCTCA TAACAAGGTT CTTTCGCCTG 
TGCACAGAAA TGTGTGTTGA GATCAGCTAC CGAGCTCAGG CCGAGCAGCA ACACAATCCC 
GCAGCCAACC CTACCATGAT CCGAGCCAAA TGCTATCATA ACCTGGATGC TTTTGTGCGA 
CTTATTGCCC TCCTGGTCAA ACATTCGGGG GAGGCCACCA ATACGGTCAC CAAGATAAAC 
CTGCTGAATA AGGTTCTTGG GATCGTGGTG GGCGTCCTCC TGCAGGATCA CGATGTTCGG 
CAAAACGATT TTCAGCAGCT TCCGTACCAC CGTATTTTCA TCATGTTGCT TTTGGAACTC 
AATGCCCCCG AGCATGTCTT GGAGACCATT AATTTTCAGA CGCTCACAGC TTTCTGCAAT 
ACATTCCACA TCTTGAGGCC TACCAAAGCT CCCGGCTTTG TGTATGCCTG GCTTGAGCTG 
ATCTCCCACC GGATATTTAT CGCACGGATG CTGGCACATA CACCACAGCA GAAGGGCTGG 
CCCATGTATG CCCAACTACT GATTGATTTG TTCAAATATT TGGCTCCCTT CCTTAGAAAT 
GTGGAACTTA CCAAACCTAT GCAAATCCTG TACAAGGGTA CTTTACGAGT GCTCCTGGTC 
TTATTGCATG ACTTTCCGGA ATTCCTTTGT GATTACCATT ATGGGTTCTG TGATGTCATC 
CCACCCAATT GCATCCAGCT AAGAAATTTG ATCCTGAGTG CCTTTCCACG CAACATGAGG 
CTCCCAGACC CATTCACTCC CAACTTAAAG GTGGATATGT TGAGTGAAAT TAACATCGCT 
CCTAGAATCC TCACCAATTT CACAGGAGTG ATGCCACCTC AGTTCAAAAA AGATCTGGAT 
TCCTATCTCA AAACCCGCTC ACCAGTCACG TTCCTTTCGG ACTTGCGCAG CAACCTGCAG 
GTATCGAATG AACCCGGTAA TCGTTACAAC ATCCAGTTGA TCAACGCCCT GGTGCTCTAC 
GTAGGTACTC AAGCCATCGC CCACATCCAC AATAAAGGTA GCACGCCATC CATGAGCACC 
ATCACCCACT CGGCTCACAT GGACATCTTC CAGAACCTGG CCGTGGACCT GGACACTGAA 
GGTCGGTACC TGTTCCTGAA TGCCATCGCT AACCAGCTGC GGTACCCGAA TAGCCACACT 
CATTACTTCA GTTGTACCAT GCTGTACCTG TTCGCAGAAG CCAACACTGA AGCCATCCAG 
GAACAGATCA CCAGGGTTCT CTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT 
CTCCTCATTA CCTTCATCGA GCTGATTAAA AACCCAGCGT TTAAGTTCTG GAACCACGAG 
TTCGTTCACT GTGCCCCAGA AATTGAAAAG TTGTTCCAGT CGGTTGCACA ATGCTGCATG 
GGGCAGAAGC AGGCTCAGCA AGTGATGGAA GGGACCGGTG CCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671975.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670165.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

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CloneID	OOc13656
Clone ID Related Accession (Same CDS sequence)	XM_007671975.1
Accession Version	XM_007671975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
ATGCACCAGC AAGGAATCTT GAAGACCGAC GACCTCATAA CAAGGTTCTT TCGCCTGTGC 
ACAGAAATGT GTGTTGAGAT CAGCTACCGA GCTCAGGCCG AGCAGCAACA CAATCCCGCA 
GCCAACCCTA CCATGATCCG AGCCAAATGC TATCATAACC TGGATGCTTT TGTGCGACTT 
ATTGCCCTCC TGGTCAAACA TTCGGGGGAG GCCACCAATA CGGTCACCAA GATAAACCTG 
CTGAATAAGG TTCTTGGGAT CGTGGTGGGC GTCCTCCTGC AGGATCACGA TGTTCGGCAA 
AACGATTTTC AGCAGCTTCC GTACCACCGT ATTTTCATCA TGTTGCTTTT GGAACTCAAT 
GCCCCCGAGC ATGTCTTGGA GACCATTAAT TTTCAGACGC TCACAGCTTT CTGCAATACA 
TTCCACATCT TGAGGCCTAC CAAAGCTCCC GGCTTTGTGT ATGCCTGGCT TGAGCTGATC 
TCCCACCGGA TATTTATCGC ACGGATGCTG GCACATACAC CACAGCAGAA GGGCTGGCCC 
ATGTATGCCC AACTACTGAT TGATTTGTTC AAATATTTGG CTCCCTTCCT TAGAAATGTG 
GAACTTACCA AACCTATGCA AATCCTGTAC AAGGGTACTT TACGAGTGCT CCTGGTCTTA 
TTGCATGACT TTCCGGAATT CCTTTGTGAT TACCATTATG GGTTCTGTGA TGTCATCCCA 
CCCAATTGCA TCCAGCTAAG AAATTTGATC CTGAGTGCCT TTCCACGCAA CATGAGGCTC 
CCAGACCCAT TCACTCCCAA CTTAAAGGTG GATATGTTGA GTGAAATTAA CATCGCTCCT 
AGAATCCTCA CCAATTTCAC AGGAGTGATG CCACCTCAGT TCAAAAAAGA TCTGGATTCC 
TATCTCAAAA CCCGCTCACC AGTCACGTTC CTTTCGGACT TGCGCAGCAA CCTGCAGGTA 
TCGAATGAAC CCGGTAATCG TTACAACATC CAGTTGATCA ACGCCCTGGT GCTCTACGTA 
GGTACTCAAG CCATCGCCCA CATCCACAAT AAAGGTAGCA CGCCATCCAT GAGCACCATC 
ACCCACTCGG CTCACATGGA CATCTTCCAG AACCTGGCCG TGGACCTGGA CACTGAAGGT 
CGGTACCTGT TCCTGAATGC CATCGCTAAC CAGCTGCGGT ACCCGAATAG CCACACTCAT 
TACTTCAGTT GTACCATGCT GTACCTGTTC GCAGAAGCCA ACACTGAAGC CATCCAGGAA 
CAGATCACCA GGGTTCTCTT GGAACGGTTG ATTGTAAATA GGCCACATCC TTGGGGTCTC 
CTCATTACCT TCATCGAGCT GATTAAAAAC CCAGCGTTTA AGTTCTGGAA CCACGAGTTC 
GTTCACTGTG CCCCAGAAAT TGAAAAGTTG TTCCAGTCGG TTGCACAATG CTGCATGGGG 
CAGAAGCAGG CTCAGCAAGT GATGGAAGGG ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670165.1
CDS	195..7319
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671975.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671975.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
ATGCACCAGC AAGGAATCTT GAAGACCGAC GACCTCATAA CAAGGTTCTT TCGCCTGTGC 
ACAGAAATGT GTGTTGAGAT CAGCTACCGA GCTCAGGCCG AGCAGCAACA CAATCCCGCA 
GCCAACCCTA CCATGATCCG AGCCAAATGC TATCATAACC TGGATGCTTT TGTGCGACTT 
ATTGCCCTCC TGGTCAAACA TTCGGGGGAG GCCACCAATA CGGTCACCAA GATAAACCTG 
CTGAATAAGG TTCTTGGGAT CGTGGTGGGC GTCCTCCTGC AGGATCACGA TGTTCGGCAA 
AACGATTTTC AGCAGCTTCC GTACCACCGT ATTTTCATCA TGTTGCTTTT GGAACTCAAT 
GCCCCCGAGC ATGTCTTGGA GACCATTAAT TTTCAGACGC TCACAGCTTT CTGCAATACA 
TTCCACATCT TGAGGCCTAC CAAAGCTCCC GGCTTTGTGT ATGCCTGGCT TGAGCTGATC 
TCCCACCGGA TATTTATCGC ACGGATGCTG GCACATACAC CACAGCAGAA GGGCTGGCCC 
ATGTATGCCC AACTACTGAT TGATTTGTTC AAATATTTGG CTCCCTTCCT TAGAAATGTG 
GAACTTACCA AACCTATGCA AATCCTGTAC AAGGGTACTT TACGAGTGCT CCTGGTCTTA 
TTGCATGACT TTCCGGAATT CCTTTGTGAT TACCATTATG GGTTCTGTGA TGTCATCCCA 
CCCAATTGCA TCCAGCTAAG AAATTTGATC CTGAGTGCCT TTCCACGCAA CATGAGGCTC 
CCAGACCCAT TCACTCCCAA CTTAAAGGTG GATATGTTGA GTGAAATTAA CATCGCTCCT 
AGAATCCTCA CCAATTTCAC AGGAGTGATG CCACCTCAGT TCAAAAAAGA TCTGGATTCC 
TATCTCAAAA CCCGCTCACC AGTCACGTTC CTTTCGGACT TGCGCAGCAA CCTGCAGGTA 
TCGAATGAAC CCGGTAATCG TTACAACATC CAGTTGATCA ACGCCCTGGT GCTCTACGTA 
GGTACTCAAG CCATCGCCCA CATCCACAAT AAAGGTAGCA CGCCATCCAT GAGCACCATC 
ACCCACTCGG CTCACATGGA CATCTTCCAG AACCTGGCCG TGGACCTGGA CACTGAAGGT 
CGGTACCTGT TCCTGAATGC CATCGCTAAC CAGCTGCGGT ACCCGAATAG CCACACTCAT 
TACTTCAGTT GTACCATGCT GTACCTGTTC GCAGAAGCCA ACACTGAAGC CATCCAGGAA 
CAGATCACCA GGGTTCTCTT GGAACGGTTG ATTGTAAATA GGCCACATCC TTGGGGTCTC 
CTCATTACCT TCATCGAGCT GATTAAAAAC CCAGCGTTTA AGTTCTGGAA CCACGAGTTC 
GTTCACTGTG CCCCAGAAAT TGAAAAGTTG TTCCAGTCGG TTGCACAATG CTGCATGGGG 
CAGAAGCAGG CTCAGCAAGT GATGGAAGGG ACCGGTGCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671976.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670166.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.

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CloneID	OOc13657
Clone ID Related Accession (Same CDS sequence)	XM_007671976.1
Accession Version	XM_007671976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCGT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670166.1
CDS	195..7307
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671976.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671976.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGTAGGTGGT 
CTCGGTACAG GCAGCCTGAC CGGTATAGGA ACTGCCGCTC TTGGGCTCCC CGCAGTGAAT 
AATGATCCGT TTGTGCAGAG GAAACTGGGC ACATCTGGAC TGAACCAGCC TACATTCCAG 
CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGCAA AGAGATAGAT 
GATGAAGCAA ACAGCTATTT TCAGCGTATT TATAACCATC CACCACACCC AACCATGTCT 
GTTGATGAGG TTTTAGAAAT GCTACAGAGG TTTAAAGACT CCAACATAAA ACGGGAACGA 
GAAGTGTTTA ACTGTATGTT GAGGAACTTG TTTGAAGAGT ATCGGTTTTT CCCCCAGTAC 
CCAGATAAAG AGCTGCATAT AACAGCCTGC CTCTTTGGTG GGATAATAGA AAAAGGACTG 
GTCACTTACA TGGCTCTGGG TCTTGCCCTG CGCTATGTTC TTGAAGCCTT ACGCAAGCCG 
TTTGCATCCA AAATGTATTA TTTTGGAATC GCTGCACTAG ATAGGTTTAA AAATAGATTG 
AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCTATTA GTCACTTTAT ACAGTTTCCT 
CATCATCTAC AGGAGTACAT TGAATACGGA CAGCAATCTA GAGACCCTCC TGTAAAAATG 
CAGGGTTCCA TCACCACCCC TGGAAGTATT GCACTTGCCC AGGCTCAGGC CCAGGCCCAG 
GTTCCAGCAA AAGCTCCTCT TGCTGGTCAA GTCAGCACTA TAGTAACCAC CTCTACCACC 
ACCACTGCTA AAACCATCAC GATCACCCGA CCAACAGGAG TCAGCTTCAA AAAGGATGTG 
CCACCTTCTA TTAATACTAC CAACATAGAT ACACTGCTAG TGGCGACCGA TCAAACTGAG 
AGAATTGTGG AACCACCAGA AAATGTGCAA GAGAAAATTG CTTTCATCTT CAACAATCTC 
TCTCAATCAA ACATGACACA GAAGGTTGAG GAGCTGAAAG AAACGGTAAA AGAGGAATTT 
ATGCCTTGGG TTTCACAATA CCTGGTAATG AAGAGAGTCA GTATTGAGCC AAACTTCCAC 
AGCTTGTATT CAAATTTCCT CGATACTCTT AAGAACCCTG AATTTAACAA GATGGTTCTG 
AATGAAACCT ACAGAAACAT CAAAGTGCTA CTGACATCAG ATAAAGCTGC AGCCAATTTT 
TCAGACCGTT CCCTACTGAA GAACCTGGGC CACTGGCTGG GAATGATTAC ACTAGCTAAA 
AATAAACCCA TCTTGCACAC TGACTTGGAT GTAAAATCTT TGCTGCTCGA AGCTTACGTT 
AAAGGACAAC AAGAATTGCT TTATGTGGTG CCCTTTGTTG CCAAAGTACT GGAATCCAGT 
GTTCGAAGTG TGGTGTTCAG GCCACCAAAC CCCTGGACTA TGGCTATCAT GAACGTCTTA 
GCCGAGTTAC ATCAAGAACA TGACCTGAAG CTAAACTTGA AATTTGAAAT TGAGGTGCTC 
TGCAAGAACC TTGCGTTAGA CATCAGTGAG CTAAAACCCG GGAACCTTCT GAAGGATAAA 
GATCGATTAA AAAACTTAGA AGAACAGCTC TCTGCTCCAA AGAAAGATGT AAAACAACCA 
GAAGAACTTC CAGCCATCAC AACAACTACG GCCTCTACAA CACCAGCCAC CAGTACGACC 
TGTACGGCGA CAGTTCCTCC TCAGCCCCAG TACAGCTACC ACGACATCAA CGTGTACTCG 
CTCGGAGGGC TGGCCCCACA CATTACTCTA AATCCCACGA TTCCGTTGTT CCAGGCCCAT 
CCACAGTTAA AGCAGTGTGT GCGCCAAGCA ATCGAACGTG CTGTACAAGA GCTTGTCCAT 
CCGGTGGTGG ATAGGTCCAT TAAGATTGCC ATGACTACTT GTGAACAAAT AGTCAGGAAG 
GATTTTGCAC TGGATTCAGA GGAGTCACGC ATGCGAATAG CAGCACATCA CATGATGAGG 
AATCTGACAG CCGGAATGGC TATGATAACT TGCAGGGAAC CTTTGCTGAT GAGTATAGCT 
ACCAACCTGA AAAACAGCTT CGCCACCGCA CTGCGGGCTG CGTCTCCTCA GCAAAGGGAA 
ATGATGGAGC AGGCAGCTGC GCAGTTAGCT CAGGACAATT GTGAATTAGC CTGCTGCTTC 
ATTCAGAAGA CTGCCGTAGA AAAAGCAGGC CCCGAAATGG ACAAGAGGCT AGCAACTGAG 
TTTGAGCTGC GCAAACACGC TAGGCAGGAG GGCCGTAGGT ACTGTGATCC CGTTGTATTA 
ACCTATCAGG CTGAGCGGAT GCCAGAACAG ATCAGGCTGA AGGTAGGAGG TGTGGACCCC 
AAACAGCTGG CTGTTTATGA GGAGTTTGCA CGCAATGTTC CTGGCTTCTT GCCTACTAAT 
GATTTAACCC AGCCCACGGG ATTCTTAGCG CAGCCTATGA AACAAGCTTG GGCAACAGAC 
GATGTAGCCC AGATTTATGA CAAGTGTATT ACAGAACTGG AACAGCACCT GCATGCCATT 
CCACCTGCAT TGGCCATGAA CCCTCAAGCT CAGGCTCTTC GCAGCCTTTT GGAAGCTGTG 
GTGCTGTCTC GCAACTCTCG TGATGCCATA GCGGCACTTG GGTTGCTTCA GAAGGCAGTA 
GAGGGTTTAC TGGATGCCAC AAGCGGTGCA GATGCCGACC TATTGCTACG TTACCGAGAG 
TGTCATCTAC TTGTACTGAA AGCGCTGCAG GATGGTCGGG CCTATGGTTC ACCGTGGTGT 
AATAAACAGA TCACGAGGTG CCTGATCGAG TGTCGGGATG AATATAAGTA TAACGTTGAA 
GCAGTGGAAC TGCTGATTCG GAACCATCTA GTTAACATGC AGCAGTATGA CCTGCACCTG 
GCTCAGTCAA TGGAGAATGG TTTGAACTAC ATGGCAGTGG CATTTGCTAT GCAGTTGGTG 
AAGATCCTGT TGGTGGATGA AAGGACTGTA GCCCATGTAA CAGAGGCAGA TTTATTTCAC 
ACCATTGAAA CTCTGATGAG GATTAATGCT CACTCCAGAG GAAATGCCCC CGAAGGATTA 
CCCCAGCTGA TGGAAGTGGT GCGTGCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA 
GGTCCTAACT TCATGATGCA CTCTGGGATC TCTCAAGCCT CAGAATATGA TGACCCTCCA 
GGCCTAAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCC 
GCAGCTGGTC GCGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCGT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671977.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670167.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.

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CloneID	OOc13658
Clone ID Related Accession (Same CDS sequence)	XM_007671977.1
Accession Version	XM_007671977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGAGCCC TTCATCCAGG CGTGTATGAC TTTCTTAAAG 
AGACGGTGCC CTTCTATCTT GGGTGGCCTT GCCCCAGAGA AGGATCAGCC CAAAAGCGCC 
CAACTTCCTC CAGAAACACT GGCAACAATG TTGGCCTGTC TTCAAGCGTG TGCCGGGAGT 
GTTTCTCAAG AGCTTTCGGA GACTATCCTC ACTATGGTGG CCAACTGCAG CAATGTTATG 
AACAAGGCTA GACAGCCACC CCCCGGAGTC ATGCCAAAAG GACGCCCTCC CAGTGCAAGC 
AGCTTAGACG CCATTTCTCC CGTACAGATT GACTCTCTTG GTGGGATGGC ATCTCTTAGT 
ATAGGTGGTT CAGCTGCCCC CCACACCCAG AGTATGCAGG GTTTTCCTCC AAATTTGGGT 
TCTGCCTTCA GTACCCCTCA GTCACCAGCA AAAGCATTTC CACCCCTTTC TACCCCAAAT 
CAGACCACAG CTTTCAGCGG TATCGGAGGG CTCTCTTCAC AACTTCCAGT AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CGTACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670167.1
CDS	195..7220
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671977.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671977.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGAGCCC TTCATCCAGG CGTGTATGAC TTTCTTAAAG 
AGACGGTGCC CTTCTATCTT GGGTGGCCTT GCCCCAGAGA AGGATCAGCC CAAAAGCGCC 
CAACTTCCTC CAGAAACACT GGCAACAATG TTGGCCTGTC TTCAAGCGTG TGCCGGGAGT 
GTTTCTCAAG AGCTTTCGGA GACTATCCTC ACTATGGTGG CCAACTGCAG CAATGTTATG 
AACAAGGCTA GACAGCCACC CCCCGGAGTC ATGCCAAAAG GACGCCCTCC CAGTGCAAGC 
AGCTTAGACG CCATTTCTCC CGTACAGATT GACTCTCTTG GTGGGATGGC ATCTCTTAGT 
ATAGGTGGTT CAGCTGCCCC CCACACCCAG AGTATGCAGG GTTTTCCTCC AAATTTGGGT 
TCTGCCTTCA GTACCCCTCA GTCACCAGCA AAAGCATTTC CACCCCTTTC TACCCCAAAT 
CAGACCACAG CTTTCAGCGG TATCGGAGGG CTCTCTTCAC AACTTCCAGT AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGC 
AAAGAGATAG ATGATGAAGC AAACAGCTAT TTTCAGCGTA TTTATAACCA TCCACCACAC 
CCAACCATGT CTGTTGATGA GGTTTTAGAA ATGCTACAGA GGTTTAAAGA CTCCAACATA 
AAACGGGAAC GAGAAGTGTT TAACTGTATG TTGAGGAACT TGTTTGAAGA GTATCGGTTT 
TTCCCCCAGT ACCCAGATAA AGAGCTGCAT ATAACAGCCT GCCTCTTTGG TGGGATAATA 
GAAAAAGGAC TGGTCACTTA CATGGCTCTG GGTCTTGCCC TGCGCTATGT TCTTGAAGCC 
TTACGCAAGC CGTTTGCATC CAAAATGTAT TATTTTGGAA TCGCTGCACT AGATAGGTTT 
AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCTAT TAGTCACTTT 
ATACAGTTTC CTCATCATCT ACAGGAGTAC ATTGAATACG GACAGCAATC TAGAGACCCT 
CCTGTAAAAA TGCAGGGTTC CATCACCACC CCTGGAAGTA TTGCACTTGC CCAGGCTCAG 
GCCCAGGCCC AGGTTCCAGC AAAAGCTCCT CTTGCTGGTC AAGTCAGCAC TATAGTAACC 
ACCTCTACCA CCACCACTGC TAAAACCATC ACGATCACCC GACCAACAGG AGTCAGCTTC 
AAAAAGGATG TGCCACCTTC TATTAATACT ACCAACATAG ATACACTGCT AGTGGCGACC 
GATCAAACTG AGAGAATTGT GGAACCACCA GAAAATGTGC AAGAGAAAAT TGCTTTCATC 
TTCAACAATC TCTCTCAATC AAACATGACA CAGAAGGTTG AGGAGCTGAA AGAAACGGTA 
AAAGAGGAAT TTATGCCTTG GGTTTCACAA TACCTGGTAA TGAAGAGAGT CAGTATTGAG 
CCAAACTTCC ACAGCTTGTA TTCAAATTTC CTCGATACTC TTAAGAACCC TGAATTTAAC 
AAGATGGTTC TGAATGAAAC CTACAGAAAC ATCAAAGTGC TACTGACATC AGATAAAGCT 
GCAGCCAATT TTTCAGACCG TTCCCTACTG AAGAACCTGG GCCACTGGCT GGGAATGATT 
ACACTAGCTA AAAATAAACC CATCTTGCAC ACTGACTTGG ATGTAAAATC TTTGCTGCTC 
GAAGCTTACG TTAAAGGACA ACAAGAATTG CTTTATGTGG TGCCCTTTGT TGCCAAAGTA 
CTGGAATCCA GTGTTCGAAG TGTGGTGTTC AGGCCACCAA ACCCCTGGAC TATGGCTATC 
ATGAACGTCT TAGCCGAGTT ACATCAAGAA CATGACCTGA AGCTAAACTT GAAATTTGAA 
ATTGAGGTGC TCTGCAAGAA CCTTGCGTTA GACATCAGTG AGCTAAAACC CGGGAACCTT 
CTGAAGGATA AAGATCGATT AAAAAACTTA GAAGAACAGC TCTCTGCTCC AAAGAAAGAT 
GTAAAACAAC CAGAAGAACT TCCAGCCATC ACAACAACTA CGGCCTCTAC AACACCAGCC 
ACCAGTACGA CCTGTACGGC GACAGTTCCT CCTCAGCCCC AGTACAGCTA CCACGACATC 
AACGTGTACT CGCTCGGAGG GCTGGCCCCA CACATTACTC TAAATCCCAC GATTCCGTTG 
TTCCAGGCCC ATCCACAGTT AAAGCAGTGT GTGCGCCAAG CAATCGAACG TGCTGTACAA 
GAGCTTGTCC ATCCGGTGGT GGATAGGTCC ATTAAGATTG CCATGACTAC TTGTGAACAA 
ATAGTCAGGA AGGATTTTGC ACTGGATTCA GAGGAGTCAC GCATGCGAAT AGCAGCACAT 
CACATGATGA GGAATCTGAC AGCCGGAATG GCTATGATAA CTTGCAGGGA ACCTTTGCTG 
ATGAGTATAG CTACCAACCT GAAAAACAGC TTCGCCACCG CACTGCGGGC TGCGTCTCCT 
CAGCAAAGGG AAATGATGGA GCAGGCAGCT GCGCAGTTAG CTCAGGACAA TTGTGAATTA 
GCCTGCTGCT TCATTCAGAA GACTGCCGTA GAAAAAGCAG GCCCCGAAAT GGACAAGAGG 
CTAGCAACTG AGTTTGAGCT GCGCAAACAC GCTAGGCAGG AGGGCCGTAG GTACTGTGAT 
CCCGTTGTAT TAACCTATCA GGCTGAGCGG ATGCCAGAAC AGATCAGGCT GAAGGTAGGA 
GGTGTGGACC CCAAACAGCT GGCTGTTTAT GAGGAGTTTG CACGCAATGT TCCTGGCTTC 
TTGCCTACTA ATGATTTAAC CCAGCCCACG GGATTCTTAG CGCAGCCTAT GAAACAAGCT 
TGGGCAACAG ACGATGTAGC CCAGATTTAT GACAAGTGTA TTACAGAACT GGAACAGCAC 
CTGCATGCCA TTCCACCTGC ATTGGCCATG AACCCTCAAG CTCAGGCTCT TCGCAGCCTT 
TTGGAAGCTG TGGTGCTGTC TCGCAACTCT CGTGATGCCA TAGCGGCACT TGGGTTGCTT 
CAGAAGGCAG TAGAGGGTTT ACTGGATGCC ACAAGCGGTG CAGATGCCGA CCTATTGCTA 
CGTTACCGAG AGTGTCATCT ACTTGTACTG AAAGCGCTGC AGGATGGTCG GGCCTATGGT 
TCACCGTGGT GTAATAAACA GATCACGAGG TGCCTGATCG AGTGTCGGGA TGAATATAAG 
TATAACGTTG AAGCAGTGGA ACTGCTGATT CGGAACCATC TAGTTAACAT GCAGCAGTAT 
GACCTGCACC TGGCTCAGTC AATGGAGAAT GGTTTGAACT ACATGGCAGT GGCATTTGCT 
ATGCAGTTGG TGAAGATCCT GTTGGTGGAT GAAAGGACTG TAGCCCATGT AACAGAGGCA 
GATTTATTTC ACACCATTGA AACTCTGATG AGGATTAATG CTCACTCCAG AGGAAATGCC 
CCCGAAGGAT TACCCCAGCT GATGGAAGTG GTGCGTGCCA ACTATGAAGC AATGATTGAT 
CGTGCTCATG GAGGTCCTAA CTTCATGATG CACTCTGGGA TCTCTCAAGC CTCAGAATAT 
GATGACCCTC CAGGCCTAAG GGAGAAGGCA GAGTATCTTC TGAGGGAATG GGTGAATCTC 
TACCATTCAG CCGCAGCTGG TCGCGACAGT ACCAAAGCTT TCTCTGCGTT TGTTGGACAG 
GTAGAGCTTT TGGAAAGAAA GATGCACCAG CAAGGAATCT TGAAGACCGA CGACCTCATA 
ACAAGGTTCT TTCGCCTGTG CACAGAAATG TGTGTTGAGA TCAGCTACCG AGCTCAGGCC 
GAGCAGCAAC ACAATCCCGC AGCCAACCCT ACCATGATCC GAGCCAAATG CTATCATAAC 
CTGGATGCTT TTGTGCGACT TATTGCCCTC CTGGTCAAAC ATTCGGGGGA GGCCACCAAT 
ACGGTCACCA AGATAAACCT GCTGAATAAG GTTCTTGGGA TCGTGGTGGG CGTCCTCCTG 
CAGGATCACG ATGTTCGGCA AAACGATTTT CAGCAGCTTC CGTACCACCG TATTTTCATC 
ATGTTGCTTT TGGAACTCAA TGCCCCCGAG CATGTCTTGG AGACCATTAA TTTTCAGACG 
CTCACAGCTT TCTGCAATAC ATTCCACATC TTGAGGCCTA CCAAAGCTCC CGGCTTTGTG 
TATGCCTGGC TTGAGCTGAT CTCCCACCGG ATATTTATCG CACGGATGCT GGCACATACA 
CCACAGCAGA AGGGCTGGCC CATGTATGCC CAACTACTGA TTGATTTGTT CAAATATTTG 
GCTCCCTTCC TTAGAAATGT GGAACTTACC AAACCTATGC AAATCCTGTA CAAGGGTACT 
TTACGAGTGC TCCTGGTCTT ATTGCATGAC TTTCCGGAAT TCCTTTGTGA TTACCATTAT 
GGGTTCTGTG ATGTCATCCC ACCCAATTGC ATCCAGCTAA GAAATTTGAT CCTGAGTGCC 
TTTCCACGCA ACATGAGGCT CCCAGACCCA TTCACTCCCA ACTTAAAGGT GGATATGTTG 
AGTGAAATTA ACATCGCTCC TAGAATCCTC ACCAATTTCA CAGGAGTGAT GCCACCTCAG 
TTCAAAAAAG ATCTGGATTC CTATCTCAAA ACCCGCTCAC CAGTCACGTT CCTTTCGGAC 
TTGCGCAGCA ACCTGCAGGT ATCGAATGAA CCCGGTAATC GTTACAACAT CCAGTTGATC 
AACGCCCTGG TGCTCTACGT AGGTACTCAA GCCATCGCCC ACATCCACAA TAAAGGTAGC 
ACGCCATCCA TGAGCACCAT CACCCACTCG GCTCACATGG ACATCTTCCA GAACCTGGCC 
GTGGACCTGG ACACTGAAGG TCGGTACCTG TTCCTGAATG CCATCGCTAA CCAGCTGCGG 
TACCCGAATA GCCACACTCA TTACTTCAGT TGTACCATGC TGTACCTGTT CGCAGAAGCC 
AACACTGAAG CCATCCAGGA ACAGATCACC AGGGTTCTCT TGGAACGGTT GATTGTAAAT 
AGGCCACATC CTTGGGGTCT CCTCATTACC TTCATCGAGC TGATTAAAAA CCCAGCGTTT 
AAGTTCTGGA ACCACGAGTT CGTTCACTGT GCCCCAGAAA TTGAAAAGTT GTTCCAGTCG 
GTTGCACAAT GCTGCATGGG GCAGAAGCAG GCTCAGCAAG TGATGGAAGG GACCGGTGCC 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671978.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670168.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.

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CloneID	OOc13659
Clone ID Related Accession (Same CDS sequence)	XM_007671978.1
Accession Version	XM_007671978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGTAGG TGGTCTCGGT ACAGGCAGCC TGACCGGTAT AGGAACTGCC 
GCTCTTGGGC TCCCCGCAGT GAATAATGAT CCGTTTGTGC AGAGGAAACT GGGCACATCT 
GGACTGAACC AGCCTACATT CCAGCAGAGT AAGATGAAAC CTTCGGACTT GTCTCAGGTG 
TGGCCAGAGG CAAATCAGCA CTTTAGCAAA GAGATAGATG ATGAAGCAAA CAGCTATTTT 
CAGCGTATTT ATAACCATCC ACCACACCCA ACCATGTCTG TTGATGAGGT TTTAGAAATG 
CTACAGAGGT TTAAAGACTC CAACATAAAA CGGGAACGAG AAGTGTTTAA CTGTATGTTG 
AGGAACTTGT TTGAAGAGTA TCGGTTTTTC CCCCAGTACC CAGATAAAGA GCTGCATATA 
ACAGCCTGCC TCTTTGGTGG GATAATAGAA AAAGGACTGG TCACTTACAT GGCTCTGGGT 
CTTGCCCTGC GCTATGTTCT TGAAGCCTTA CGCAAGCCGT TTGCATCCAA AATGTATTAT 
TTTGGAATCG CTGCACTAGA TAGGTTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT 
CAGCACTTGG CTTCTATTAG TCACTTTATA CAGTTTCCTC ATCATCTACA GGAGTACATT 
GAATACGGAC AGCAATCTAG AGACCCTCCT GTAAAAATGC AGGGTTCCAT CACCACCCCT 
GGAAGTATTG CACTTGCCCA GGCTCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCTCTT 
GCTGGTCAAG TCAGCACTAT AGTAACCACC TCTACCACCA CCACTGCTAA AACCATCACG 
ATCACCCGAC CAACAGGAGT CAGCTTCAAA AAGGATGTGC CACCTTCTAT TAATACTACC 
AACATAGATA CACTGCTAGT GGCGACCGAT CAAACTGAGA GAATTGTGGA ACCACCAGAA 
AATGTGCAAG AGAAAATTGC TTTCATCTTC AACAATCTCT CTCAATCAAA CATGACACAG 
AAGGTTGAGG AGCTGAAAGA AACGGTAAAA GAGGAATTTA TGCCTTGGGT TTCACAATAC 
CTGGTAATGA AGAGAGTCAG TATTGAGCCA AACTTCCACA GCTTGTATTC AAATTTCCTC 
GATACTCTTA AGAACCCTGA ATTTAACAAG ATGGTTCTGA ATGAAACCTA CAGAAACATC 
AAAGTGCTAC TGACATCAGA TAAAGCTGCA GCCAATTTTT CAGACCGTTC CCTACTGAAG 
AACCTGGGCC ACTGGCTGGG AATGATTACA CTAGCTAAAA ATAAACCCAT CTTGCACACT 
GACTTGGATG TAAAATCTTT GCTGCTCGAA GCTTACGTTA AAGGACAACA AGAATTGCTT 
TATGTGGTGC CCTTTGTTGC CAAAGTACTG GAATCCAGTG TTCGAAGTGT GGTGTTCAGG 
CCACCAAACC CCTGGACTAT GGCTATCATG AACGTCTTAG CCGAGTTACA TCAAGAACAT 
GACCTGAAGC TAAACTTGAA ATTTGAAATT GAGGTGCTCT GCAAGAACCT TGCGTTAGAC 
ATCAGTGAGC TAAAACCCGG GAACCTTCTG AAGGATAAAG ATCGATTAAA AAACTTAGAA 
GAACAGCTCT CTGCTCCAAA GAAAGATGTA AAACAACCAG AAGAACTTCC AGCCATCACA 
ACAACTACGG CCTCTACAAC ACCAGCCACC AGTACGACCT GTACGGCGAC AGTTCCTCCT 
CAGCCCCAGT ACAGCTACCA CGACATCAAC GTGTACTCGC TCGGAGGGCT GGCCCCACAC 
ATTACTCTAA ATCCCACGAT TCCGTTGTTC CAGGCCCATC CACAGTTAAA GCAGTGTGTG 
CGCCAAGCAA TCGAACGTGC TGTACAAGAG CTTGTCCATC CGGTGGTGGA TAGGTCCATT 
AAGATTGCCA TGACTACTTG TGAACAAATA GTCAGGAAGG ATTTTGCACT GGATTCAGAG 
GAGTCACGCA TGCGAATAGC AGCACATCAC ATGATGAGGA ATCTGACAGC CGGAATGGCT 
ATGATAACTT GCAGGGAACC TTTGCTGATG AGTATAGCTA CCAACCTGAA AAACAGCTTC 
GCCACCGCAC TGCGGGCTGC GTCTCCTCAG CAAAGGGAAA TGATGGAGCA GGCAGCTGCG 
CAGTTAGCTC AGGACAATTG TGAATTAGCC TGCTGCTTCA TTCAGAAGAC TGCCGTAGAA 
AAAGCAGGCC CCGAAATGGA CAAGAGGCTA GCAACTGAGT TTGAGCTGCG CAAACACGCT 
AGGCAGGAGG GCCGTAGGTA CTGTGATCCC GTTGTATTAA CCTATCAGGC TGAGCGGATG 
CCAGAACAGA TCAGGCTGAA GGTAGGAGGT GTGGACCCCA AACAGCTGGC TGTTTATGAG 
GAGTTTGCAC GCAATGTTCC TGGCTTCTTG CCTACTAATG ATTTAACCCA GCCCACGGGA 
TTCTTAGCGC AGCCTATGAA ACAAGCTTGG GCAACAGACG ATGTAGCCCA GATTTATGAC 
AAGTGTATTA CAGAACTGGA ACAGCACCTG CATGCCATTC CACCTGCATT GGCCATGAAC 
CCTCAAGCTC AGGCTCTTCG CAGCCTTTTG GAAGCTGTGG TGCTGTCTCG CAACTCTCGT 
GATGCCATAG CGGCACTTGG GTTGCTTCAG AAGGCAGTAG AGGGTTTACT GGATGCCACA 
AGCGGTGCAG ATGCCGACCT ATTGCTACGT TACCGAGAGT GTCATCTACT TGTACTGAAA 
GCGCTGCAGG ATGGTCGGGC CTATGGTTCA CCGTGGTGTA ATAAACAGAT CACGAGGTGC 
CTGATCGAGT GTCGGGATGA ATATAAGTAT AACGTTGAAG CAGTGGAACT GCTGATTCGG 
AACCATCTAG TTAACATGCA GCAGTATGAC CTGCACCTGG CTCAGTCAAT GGAGAATGGT 
TTGAACTACA TGGCAGTGGC ATTTGCTATG CAGTTGGTGA AGATCCTGTT GGTGGATGAA 
AGGACTGTAG CCCATGTAAC AGAGGCAGAT TTATTTCACA CCATTGAAAC TCTGATGAGG 
ATTAATGCTC ACTCCAGAGG AAATGCCCCC GAAGGATTAC CCCAGCTGAT GGAAGTGGTG 
CGTGCCAACT ATGAAGCAAT GATTGATCGT GCTCATGGAG GTCCTAACTT CATGATGCAC 
TCTGGGATCT CTCAAGCCTC AGAATATGAT GACCCTCCAG GCCTAAGGGA GAAGGCAGAG 
TATCTTCTGA GGGAATGGGT GAATCTCTAC CATTCAGCCG CAGCTGGTCG CGACAGTACC 
AAAGCTTTCT CTGCGTTTGT TGGACAGGTA GAGCTTTTGG AAAGAAAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCGT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670168.1
CDS	195..7187
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQVELLERKMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671978.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671978.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ACCACAGCTT TCAGCGGTAT CGGAGGGCTC 
TCTTCACAAC TTCCAGTAGG TGGTCTCGGT ACAGGCAGCC TGACCGGTAT AGGAACTGCC 
GCTCTTGGGC TCCCCGCAGT GAATAATGAT CCGTTTGTGC AGAGGAAACT GGGCACATCT 
GGACTGAACC AGCCTACATT CCAGCAGAGT AAGATGAAAC CTTCGGACTT GTCTCAGGTG 
TGGCCAGAGG CAAATCAGCA CTTTAGCAAA GAGATAGATG ATGAAGCAAA CAGCTATTTT 
CAGCGTATTT ATAACCATCC ACCACACCCA ACCATGTCTG TTGATGAGGT TTTAGAAATG 
CTACAGAGGT TTAAAGACTC CAACATAAAA CGGGAACGAG AAGTGTTTAA CTGTATGTTG 
AGGAACTTGT TTGAAGAGTA TCGGTTTTTC CCCCAGTACC CAGATAAAGA GCTGCATATA 
ACAGCCTGCC TCTTTGGTGG GATAATAGAA AAAGGACTGG TCACTTACAT GGCTCTGGGT 
CTTGCCCTGC GCTATGTTCT TGAAGCCTTA CGCAAGCCGT TTGCATCCAA AATGTATTAT 
TTTGGAATCG CTGCACTAGA TAGGTTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT 
CAGCACTTGG CTTCTATTAG TCACTTTATA CAGTTTCCTC ATCATCTACA GGAGTACATT 
GAATACGGAC AGCAATCTAG AGACCCTCCT GTAAAAATGC AGGGTTCCAT CACCACCCCT 
GGAAGTATTG CACTTGCCCA GGCTCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCTCTT 
GCTGGTCAAG TCAGCACTAT AGTAACCACC TCTACCACCA CCACTGCTAA AACCATCACG 
ATCACCCGAC CAACAGGAGT CAGCTTCAAA AAGGATGTGC CACCTTCTAT TAATACTACC 
AACATAGATA CACTGCTAGT GGCGACCGAT CAAACTGAGA GAATTGTGGA ACCACCAGAA 
AATGTGCAAG AGAAAATTGC TTTCATCTTC AACAATCTCT CTCAATCAAA CATGACACAG 
AAGGTTGAGG AGCTGAAAGA AACGGTAAAA GAGGAATTTA TGCCTTGGGT TTCACAATAC 
CTGGTAATGA AGAGAGTCAG TATTGAGCCA AACTTCCACA GCTTGTATTC AAATTTCCTC 
GATACTCTTA AGAACCCTGA ATTTAACAAG ATGGTTCTGA ATGAAACCTA CAGAAACATC 
AAAGTGCTAC TGACATCAGA TAAAGCTGCA GCCAATTTTT CAGACCGTTC CCTACTGAAG 
AACCTGGGCC ACTGGCTGGG AATGATTACA CTAGCTAAAA ATAAACCCAT CTTGCACACT 
GACTTGGATG TAAAATCTTT GCTGCTCGAA GCTTACGTTA AAGGACAACA AGAATTGCTT 
TATGTGGTGC CCTTTGTTGC CAAAGTACTG GAATCCAGTG TTCGAAGTGT GGTGTTCAGG 
CCACCAAACC CCTGGACTAT GGCTATCATG AACGTCTTAG CCGAGTTACA TCAAGAACAT 
GACCTGAAGC TAAACTTGAA ATTTGAAATT GAGGTGCTCT GCAAGAACCT TGCGTTAGAC 
ATCAGTGAGC TAAAACCCGG GAACCTTCTG AAGGATAAAG ATCGATTAAA AAACTTAGAA 
GAACAGCTCT CTGCTCCAAA GAAAGATGTA AAACAACCAG AAGAACTTCC AGCCATCACA 
ACAACTACGG CCTCTACAAC ACCAGCCACC AGTACGACCT GTACGGCGAC AGTTCCTCCT 
CAGCCCCAGT ACAGCTACCA CGACATCAAC GTGTACTCGC TCGGAGGGCT GGCCCCACAC 
ATTACTCTAA ATCCCACGAT TCCGTTGTTC CAGGCCCATC CACAGTTAAA GCAGTGTGTG 
CGCCAAGCAA TCGAACGTGC TGTACAAGAG CTTGTCCATC CGGTGGTGGA TAGGTCCATT 
AAGATTGCCA TGACTACTTG TGAACAAATA GTCAGGAAGG ATTTTGCACT GGATTCAGAG 
GAGTCACGCA TGCGAATAGC AGCACATCAC ATGATGAGGA ATCTGACAGC CGGAATGGCT 
ATGATAACTT GCAGGGAACC TTTGCTGATG AGTATAGCTA CCAACCTGAA AAACAGCTTC 
GCCACCGCAC TGCGGGCTGC GTCTCCTCAG CAAAGGGAAA TGATGGAGCA GGCAGCTGCG 
CAGTTAGCTC AGGACAATTG TGAATTAGCC TGCTGCTTCA TTCAGAAGAC TGCCGTAGAA 
AAAGCAGGCC CCGAAATGGA CAAGAGGCTA GCAACTGAGT TTGAGCTGCG CAAACACGCT 
AGGCAGGAGG GCCGTAGGTA CTGTGATCCC GTTGTATTAA CCTATCAGGC TGAGCGGATG 
CCAGAACAGA TCAGGCTGAA GGTAGGAGGT GTGGACCCCA AACAGCTGGC TGTTTATGAG 
GAGTTTGCAC GCAATGTTCC TGGCTTCTTG CCTACTAATG ATTTAACCCA GCCCACGGGA 
TTCTTAGCGC AGCCTATGAA ACAAGCTTGG GCAACAGACG ATGTAGCCCA GATTTATGAC 
AAGTGTATTA CAGAACTGGA ACAGCACCTG CATGCCATTC CACCTGCATT GGCCATGAAC 
CCTCAAGCTC AGGCTCTTCG CAGCCTTTTG GAAGCTGTGG TGCTGTCTCG CAACTCTCGT 
GATGCCATAG CGGCACTTGG GTTGCTTCAG AAGGCAGTAG AGGGTTTACT GGATGCCACA 
AGCGGTGCAG ATGCCGACCT ATTGCTACGT TACCGAGAGT GTCATCTACT TGTACTGAAA 
GCGCTGCAGG ATGGTCGGGC CTATGGTTCA CCGTGGTGTA ATAAACAGAT CACGAGGTGC 
CTGATCGAGT GTCGGGATGA ATATAAGTAT AACGTTGAAG CAGTGGAACT GCTGATTCGG 
AACCATCTAG TTAACATGCA GCAGTATGAC CTGCACCTGG CTCAGTCAAT GGAGAATGGT 
TTGAACTACA TGGCAGTGGC ATTTGCTATG CAGTTGGTGA AGATCCTGTT GGTGGATGAA 
AGGACTGTAG CCCATGTAAC AGAGGCAGAT TTATTTCACA CCATTGAAAC TCTGATGAGG 
ATTAATGCTC ACTCCAGAGG AAATGCCCCC GAAGGATTAC CCCAGCTGAT GGAAGTGGTG 
CGTGCCAACT ATGAAGCAAT GATTGATCGT GCTCATGGAG GTCCTAACTT CATGATGCAC 
TCTGGGATCT CTCAAGCCTC AGAATATGAT GACCCTCCAG GCCTAAGGGA GAAGGCAGAG 
TATCTTCTGA GGGAATGGGT GAATCTCTAC CATTCAGCCG CAGCTGGTCG CGACAGTACC 
AAAGCTTTCT CTGCGTTTGT TGGACAGGTA GAGCTTTTGG AAAGAAAGAT GCACCAGCAA 
GGAATCTTGA AGACCGACGA CCTCATAACA AGGTTCTTTC GCCTGTGCAC AGAAATGTGT 
GTTGAGATCA GCTACCGAGC TCAGGCCGAG CAGCAACACA ATCCCGCAGC CAACCCTACC 
ATGATCCGAG CCAAATGCTA TCATAACCTG GATGCTTTTG TGCGACTTAT TGCCCTCCTG 
GTCAAACATT CGGGGGAGGC CACCAATACG GTCACCAAGA TAAACCTGCT GAATAAGGTT 
CTTGGGATCG TGGTGGGCGT CCTCCTGCAG GATCACGATG TTCGGCAAAA CGATTTTCAG 
CAGCTTCCGT ACCACCGTAT TTTCATCATG TTGCTTTTGG AACTCAATGC CCCCGAGCAT 
GTCTTGGAGA CCATTAATTT TCAGACGCTC ACAGCTTTCT GCAATACATT CCACATCTTG 
AGGCCTACCA AAGCTCCCGG CTTTGTGTAT GCCTGGCTTG AGCTGATCTC CCACCGGATA 
TTTATCGCAC GGATGCTGGC ACATACACCA CAGCAGAAGG GCTGGCCCAT GTATGCCCAA 
CTACTGATTG ATTTGTTCAA ATATTTGGCT CCCTTCCTTA GAAATGTGGA ACTTACCAAA 
CCTATGCAAA TCCTGTACAA GGGTACTTTA CGAGTGCTCC TGGTCTTATT GCATGACTTT 
CCGGAATTCC TTTGTGATTA CCATTATGGG TTCTGTGATG TCATCCCACC CAATTGCATC 
CAGCTAAGAA ATTTGATCCT GAGTGCCTTT CCACGCAACA TGAGGCTCCC AGACCCATTC 
ACTCCCAACT TAAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCTAG AATCCTCACC 
AATTTCACAG GAGTGATGCC ACCTCAGTTC AAAAAAGATC TGGATTCCTA TCTCAAAACC 
CGCTCACCAG TCACGTTCCT TTCGGACTTG CGCAGCAACC TGCAGGTATC GAATGAACCC 
GGTAATCGTT ACAACATCCA GTTGATCAAC GCCCTGGTGC TCTACGTAGG TACTCAAGCC 
ATCGCCCACA TCCACAATAA AGGTAGCACG CCATCCATGA GCACCATCAC CCACTCGGCT 
CACATGGACA TCTTCCAGAA CCTGGCCGTG GACCTGGACA CTGAAGGTCG GTACCTGTTC 
CTGAATGCCA TCGCTAACCA GCTGCGGTAC CCGAATAGCC ACACTCATTA CTTCAGTTGT 
ACCATGCTGT ACCTGTTCGC AGAAGCCAAC ACTGAAGCCA TCCAGGAACA GATCACCAGG 
GTTCTCTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTCCT CATTACCTTC 
ATCGAGCTGA TTAAAAACCC AGCGTTTAAG TTCTGGAACC ACGAGTTCGT TCACTGTGCC 
CCAGAAATTG AAAAGTTGTT CCAGTCGGTT GCACAATGCT GCATGGGGCA GAAGCAGGCT 
CAGCAAGTGA TGGAAGGGAC CGGTGCCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNOT1 ( XM_007671979.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007670169.1 Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.

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CloneID	OOc13660
Clone ID Related Accession (Same CDS sequence)	XM_007671979.1
Accession Version	XM_007671979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	CCR4-NOT transcription complex subunit 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794492.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCGTACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007670169.1
CDS	195..7304
Translation	MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPSPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGIAKTMMESSLADFMQEVGYGFCASIEECRSIIRQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLQNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFNFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDSLGGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTAALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFASKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFIQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTIVTTSTTTTAKTITITRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSVRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDISELKPGNLLKDKDRLKNLEEQLSAPKKDVKQPEELPAITTTTASTTPATSTTCTATVPPQPQYSYHDINVYSLGGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFATALRAASPQQREMMEQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLTQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPALAMNPQAQALRSLLEAVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERTVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRANYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQNDFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNIQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS

Target ORF information:

RefSeq Version	XM_007671979.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007671979.1

ORF Insert Sequence:

ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGC 
CCCGAAGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ACGTGGATTT CAGTGGCGAT 
GGTAAAAGCA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCG 
CTGATTACGA AGCCAAACTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCCTTGCAC 
TATCAGAAGA GTCTAAAGCC GTCACCCCAC TTATTTGCCC AATTGAGTAA AGTTCTCAAG 
TTAAGCAAAG TTCAGGAGGT AATTTTTGGT CTTGCGCTGT TGAATTCATC TAGCTCAGAT 
CTACGAGGTT TTGCTGCCCA GTTTATCAAA CAAAAGCTTC CAGACCTTCT GCGCTCTTAC 
ATCGACGCGG ACGTTAGCGG AAATCAAGAA GGTGGCTTCC AAGATATTGC AATAGAGGTC 
TTGCACCTCC TCCTCTCCCA TCTCCTATTT GGGCAGAAGG GAGCCTTCGG GGTTGGACAG 
GAACAGATCG ACGCATTCCT CAAGACGCTG CGTAGAGATT TTCCCCAGGA ACGCTGTCCT 
GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGGATCCTG 
CCTGACTCTG GAGGGATAGC TAAAACCATG ATGGAGAGCT CTCTTGCAGA CTTCATGCAA 
GAAGTTGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAGTATAAT CAGGCAGTTT 
GGTGTTCGGG AGGTCACAGC AGCCCAGGTC GCCAGGGTTT TGGGAATGAT GGCCCGCACT 
CATTCAGGGT TGACGGATGG AATTCCATTA CAGTCCATTT CAGCCCCTGG CAGTGGGATA 
TGGAGTGATG GAAAAGATAA AAGTGATGGT GCACAGGCCC ATACCTGGAA TGTGGAAGTC 
TTAATTGATG TCTTGAAGGA GCTGAACCCT AGTTTGAACT TCAAGGAAGT AACTTACGAA 
CTGGACCACC CTGGATTTCA AATTCGTGAC AGTAAGGGAC TGCAGAATGT GGTGTATGGC 
ATTCAGAGAG GCCTTGGCAT GGAAGTCTTC CCAGTAGATC TCATTTATAG GCCTTGGAAA 
CACGCCGAGG GCCAGCTCTC CTTCATTCAG CATTCCCTCA TAAATCCAGA GATCTTCTGT 
TTTGCTGATT ATCCCTGTCA TACTGTGGCA ACTGACATCC TGAAAGCACC ACCGGAAGAC 
GACAATCGAG AAATAGCCAC ATGGAAGAGC CTGGACTTGA TTGAATCCCT GTTGCGACTG 
GCTGAGGTGG GCCAGTATGA GCAGGTCAAA CAACTCTTCA ACTTCCCTAT CAAACACTGT 
CCGGATATGT TGGTATTAGC CTTACTGCAG ATCAACACCT CCTGGCATAC CTTGCGACAT 
GAACTCATCT CCACACTGAT GCCAATTTTC CTTGGAAACC ACCCTAATTC TGCCATTATT 
TTGCACTATG CATGGCATGG TCAGGGCCAG TCTCCTTCAA TTCGCCAACT TATCATGCAT 
GCAATGGCAG AATGGTACAT GAGAGGGGAG CAGTACGACC AGGCAAAATT ATCTCGGATT 
CTTGACGTGG CCCAAGACCT GAAGGCCTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC 
TTTGTTATTG ACCTTGCTGC ACTTGCCTCT CGTCGGGAAT ACCTCAAACT TGACAAATGG 
CTCACAGATA AAATTCGGGA GCATGGGGAG CCCTTCATCC AGGCGTGTAT GACTTTCTTA 
AAGAGACGGT GCCCTTCTAT CTTGGGTGGC CTTGCCCCAG AGAAGGATCA GCCCAAAAGC 
GCCCAACTTC CTCCAGAAAC ACTGGCAACA ATGTTGGCCT GTCTTCAAGC GTGTGCCGGG 
AGTGTTTCTC AAGAGCTTTC GGAGACTATC CTCACTATGG TGGCCAACTG CAGCAATGTT 
ATGAACAAGG CTAGACAGCC ACCCCCCGGA GTCATGCCAA AAGGACGCCC TCCCAGTGCA 
AGCAGCTTAG ACGCCATTTC TCCCGTACAG ATTGACTCTC TTGGTGGGAT GGCATCTCTT 
AGTATAGGTG GTTCAGCTGC CCCCCACACC CAGAGTATGC AGGGTTTTCC TCCAAATTTG 
GGTTCTGCCT TCAGTACCCC TCAGTCACCA GCAAAAGCAT TTCCACCCCT TTCTACCCCA 
AATCAGACCA CAGCTTTCAG CGGTATCGGA GGGCTCTCTT CACAACTTCC AGGTGGTCTC 
GGTACAGGCA GCCTGACCGG TATAGGAACT GCCGCTCTTG GGCTCCCCGC AGTGAATAAT 
GATCCGTTTG TGCAGAGGAA ACTGGGCACA TCTGGACTGA ACCAGCCTAC ATTCCAGCAG 
ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGCAAAGA GATAGATGAT 
GAAGCAAACA GCTATTTTCA GCGTATTTAT AACCATCCAC CACACCCAAC CATGTCTGTT 
GATGAGGTTT TAGAAATGCT ACAGAGGTTT AAAGACTCCA ACATAAAACG GGAACGAGAA 
GTGTTTAACT GTATGTTGAG GAACTTGTTT GAAGAGTATC GGTTTTTCCC CCAGTACCCA 
GATAAAGAGC TGCATATAAC AGCCTGCCTC TTTGGTGGGA TAATAGAAAA AGGACTGGTC 
ACTTACATGG CTCTGGGTCT TGCCCTGCGC TATGTTCTTG AAGCCTTACG CAAGCCGTTT 
GCATCCAAAA TGTATTATTT TGGAATCGCT GCACTAGATA GGTTTAAAAA TAGATTGAAG 
GACTATCCCC AGTATTGTCA GCACTTGGCT TCTATTAGTC ACTTTATACA GTTTCCTCAT 
CATCTACAGG AGTACATTGA ATACGGACAG CAATCTAGAG ACCCTCCTGT AAAAATGCAG 
GGTTCCATCA CCACCCCTGG AAGTATTGCA CTTGCCCAGG CTCAGGCCCA GGCCCAGGTT 
CCAGCAAAAG CTCCTCTTGC TGGTCAAGTC AGCACTATAG TAACCACCTC TACCACCACC 
ACTGCTAAAA CCATCACGAT CACCCGACCA ACAGGAGTCA GCTTCAAAAA GGATGTGCCA 
CCTTCTATTA ATACTACCAA CATAGATACA CTGCTAGTGG CGACCGATCA AACTGAGAGA 
ATTGTGGAAC CACCAGAAAA TGTGCAAGAG AAAATTGCTT TCATCTTCAA CAATCTCTCT 
CAATCAAACA TGACACAGAA GGTTGAGGAG CTGAAAGAAA CGGTAAAAGA GGAATTTATG 
CCTTGGGTTT CACAATACCT GGTAATGAAG AGAGTCAGTA TTGAGCCAAA CTTCCACAGC 
TTGTATTCAA ATTTCCTCGA TACTCTTAAG AACCCTGAAT TTAACAAGAT GGTTCTGAAT 
GAAACCTACA GAAACATCAA AGTGCTACTG ACATCAGATA AAGCTGCAGC CAATTTTTCA 
GACCGTTCCC TACTGAAGAA CCTGGGCCAC TGGCTGGGAA TGATTACACT AGCTAAAAAT 
AAACCCATCT TGCACACTGA CTTGGATGTA AAATCTTTGC TGCTCGAAGC TTACGTTAAA 
GGACAACAAG AATTGCTTTA TGTGGTGCCC TTTGTTGCCA AAGTACTGGA ATCCAGTGTT 
CGAAGTGTGG TGTTCAGGCC ACCAAACCCC TGGACTATGG CTATCATGAA CGTCTTAGCC 
GAGTTACATC AAGAACATGA CCTGAAGCTA AACTTGAAAT TTGAAATTGA GGTGCTCTGC 
AAGAACCTTG CGTTAGACAT CAGTGAGCTA AAACCCGGGA ACCTTCTGAA GGATAAAGAT 
CGATTAAAAA ACTTAGAAGA ACAGCTCTCT GCTCCAAAGA AAGATGTAAA ACAACCAGAA 
GAACTTCCAG CCATCACAAC AACTACGGCC TCTACAACAC CAGCCACCAG TACGACCTGT 
ACGGCGACAG TTCCTCCTCA GCCCCAGTAC AGCTACCACG ACATCAACGT GTACTCGCTC 
GGAGGGCTGG CCCCACACAT TACTCTAAAT CCCACGATTC CGTTGTTCCA GGCCCATCCA 
CAGTTAAAGC AGTGTGTGCG CCAAGCAATC GAACGTGCTG TACAAGAGCT TGTCCATCCG 
GTGGTGGATA GGTCCATTAA GATTGCCATG ACTACTTGTG AACAAATAGT CAGGAAGGAT 
TTTGCACTGG ATTCAGAGGA GTCACGCATG CGAATAGCAG CACATCACAT GATGAGGAAT 
CTGACAGCCG GAATGGCTAT GATAACTTGC AGGGAACCTT TGCTGATGAG TATAGCTACC 
AACCTGAAAA ACAGCTTCGC CACCGCACTG CGGGCTGCGT CTCCTCAGCA AAGGGAAATG 
ATGGAGCAGG CAGCTGCGCA GTTAGCTCAG GACAATTGTG AATTAGCCTG CTGCTTCATT 
CAGAAGACTG CCGTAGAAAA AGCAGGCCCC GAAATGGACA AGAGGCTAGC AACTGAGTTT 
GAGCTGCGCA AACACGCTAG GCAGGAGGGC CGTAGGTACT GTGATCCCGT TGTATTAACC 
TATCAGGCTG AGCGGATGCC AGAACAGATC AGGCTGAAGG TAGGAGGTGT GGACCCCAAA 
CAGCTGGCTG TTTATGAGGA GTTTGCACGC AATGTTCCTG GCTTCTTGCC TACTAATGAT 
TTAACCCAGC CCACGGGATT CTTAGCGCAG CCTATGAAAC AAGCTTGGGC AACAGACGAT 
GTAGCCCAGA TTTATGACAA GTGTATTACA GAACTGGAAC AGCACCTGCA TGCCATTCCA 
CCTGCATTGG CCATGAACCC TCAAGCTCAG GCTCTTCGCA GCCTTTTGGA AGCTGTGGTG 
CTGTCTCGCA ACTCTCGTGA TGCCATAGCG GCACTTGGGT TGCTTCAGAA GGCAGTAGAG 
GGTTTACTGG ATGCCACAAG CGGTGCAGAT GCCGACCTAT TGCTACGTTA CCGAGAGTGT 
CATCTACTTG TACTGAAAGC GCTGCAGGAT GGTCGGGCCT ATGGTTCACC GTGGTGTAAT 
AAACAGATCA CGAGGTGCCT GATCGAGTGT CGGGATGAAT ATAAGTATAA CGTTGAAGCA 
GTGGAACTGC TGATTCGGAA CCATCTAGTT AACATGCAGC AGTATGACCT GCACCTGGCT 
CAGTCAATGG AGAATGGTTT GAACTACATG GCAGTGGCAT TTGCTATGCA GTTGGTGAAG 
ATCCTGTTGG TGGATGAAAG GACTGTAGCC CATGTAACAG AGGCAGATTT ATTTCACACC 
ATTGAAACTC TGATGAGGAT TAATGCTCAC TCCAGAGGAA ATGCCCCCGA AGGATTACCC 
CAGCTGATGG AAGTGGTGCG TGCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGT 
CCTAACTTCA TGATGCACTC TGGGATCTCT CAAGCCTCAG AATATGATGA CCCTCCAGGC 
CTAAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCCGCA 
GCTGGTCGCG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA 
ATCTTGAAGA CCGACGACCT CATAACAAGG TTCTTTCGCC TGTGCACAGA AATGTGTGTT 
GAGATCAGCT ACCGAGCTCA GGCCGAGCAG CAACACAATC CCGCAGCCAA CCCTACCATG 
ATCCGAGCCA AATGCTATCA TAACCTGGAT GCTTTTGTGC GACTTATTGC CCTCCTGGTC 
AAACATTCGG GGGAGGCCAC CAATACGGTC ACCAAGATAA ACCTGCTGAA TAAGGTTCTT 
GGGATCGTGG TGGGCGTCCT CCTGCAGGAT CACGATGTTC GGCAAAACGA TTTTCAGCAG 
CTTCCGTACC ACCGTATTTT CATCATGTTG CTTTTGGAAC TCAATGCCCC CGAGCATGTC 
TTGGAGACCA TTAATTTTCA GACGCTCACA GCTTTCTGCA ATACATTCCA CATCTTGAGG 
CCTACCAAAG CTCCCGGCTT TGTGTATGCC TGGCTTGAGC TGATCTCCCA CCGGATATTT 
ATCGCACGGA TGCTGGCACA TACACCACAG CAGAAGGGCT GGCCCATGTA TGCCCAACTA 
CTGATTGATT TGTTCAAATA TTTGGCTCCC TTCCTTAGAA ATGTGGAACT TACCAAACCT 
ATGCAAATCC TGTACAAGGG TACTTTACGA GTGCTCCTGG TCTTATTGCA TGACTTTCCG 
GAATTCCTTT GTGATTACCA TTATGGGTTC TGTGATGTCA TCCCACCCAA TTGCATCCAG 
CTAAGAAATT TGATCCTGAG TGCCTTTCCA CGCAACATGA GGCTCCCAGA CCCATTCACT 
CCCAACTTAA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCTAGAAT CCTCACCAAT 
TTCACAGGAG TGATGCCACC TCAGTTCAAA AAAGATCTGG ATTCCTATCT CAAAACCCGC 
TCACCAGTCA CGTTCCTTTC GGACTTGCGC AGCAACCTGC AGGTATCGAA TGAACCCGGT 
AATCGTTACA ACATCCAGTT GATCAACGCC CTGGTGCTCT ACGTAGGTAC TCAAGCCATC 
GCCCACATCC ACAATAAAGG TAGCACGCCA TCCATGAGCA CCATCACCCA CTCGGCTCAC 
ATGGACATCT TCCAGAACCT GGCCGTGGAC CTGGACACTG AAGGTCGGTA CCTGTTCCTG 
AATGCCATCG CTAACCAGCT GCGGTACCCG AATAGCCACA CTCATTACTT CAGTTGTACC 
ATGCTGTACC TGTTCGCAGA AGCCAACACT GAAGCCATCC AGGAACAGAT CACCAGGGTT 
CTCTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTCCTCAT TACCTTCATC 
GAGCTGATTA AAAACCCAGC GTTTAAGTTC TGGAACCACG AGTTCGTTCA CTGTGCCCCA 
GAAATTGAAA AGTTGTTCCA GTCGGTTGCA CAATGCTGCA TGGGGCAGAA GCAGGCTCAG 
CAAGTGATGG AAGGGACCGG TGCCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CNOT1
Entrez Gene ID	100074556
Full Name	CCR4-NOT transcription complex subunit 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CNOT1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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