CACNA1I cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1I
Entrez Gene ID	100075872
Full Name	calcium voltage-gated channel subunit alpha1 I
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003431046.2	XP_003431094.1	voltage-dependent T-type calcium channel subunit alpha-1I
XM_029079137.1	XP_028934970.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
XM_029079136.1	XP_028934969.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
XM_029079134.1	XP_028934967.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
XM_029079135.1	XP_028934968.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
XM_029079138.1	XP_028934971.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X4

Pathways from BioSystems
Gene Ontology

The following CACNA1I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc116263	XM_029079134.1 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc116263	XM_029079135.1 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc116264	XM_029079136.1 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc116265	XM_029079137.1 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc116266	XM_029079138.1 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc12700	XM_003431046.2 Latest version!	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1I ( XM_029079134.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028934967.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc116263
Clone ID Related Accession (Same CDS sequence)	XM_029079134.1 , XM_029079135.1
Accession Version	XM_029079134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041741.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GAGAACTTGT GGCTGGACAG TGTCTCTTTA ATTATTAAGG ACTCCTTCGA AGGGGAGTTA 
ATGATCATCG ATAACCTTTC AGGCTCCGTC TTCCACCATT ACTCCTCGCC GGCCGTGTGC 
GAGAAGTGTA ACCATGACAC GCAAGAGGTC CAGCTGGCGG AGACGGAAGC TTTCTCCCTG 
AACTCGGACA AGTCCTCTTC CGTCCTGCTG GGGGACGACC TCAGCCTGGA TGACCCCGGC 
CTCTGCCCTC CCGGCGCCAA GGGGATCAAG GGCGAACCAG AGACCCCGGA CCCCGTCAAC 
GTGGGAGACT TGGGTGAATG TTTCTTCCCG CCGACTAACC TCGCAGTTCC TCCGCCTGCA 
GAGAGTTCTC TGGGGGAGAT GGAGACCACC CCCTTCCACC CCGTCCCTGC TACGTGGCTT 
GAGCAAGAGA GCAGCCCAGT TCCTTCGAGC CCTTTCTCTC CGGACACCCC CAGCCCCCTC 
TTGCCTGTGC CAGCCGAGTT CTTTCAGCCG GTCGTCTCCG CCAGCCAGCA GGGCCAGGAG 
AAAACTGCCC GCCCCGGGAG CCTCCCCAAG ATCTCCCTGC AGGGCTCCTG GGCGACCCTG 
CGCCCCCCAA CCGTGAACTG CACGCTTCTC CGTCAGCCCC CCGAGAGCGA CCCCTCCCTG 
GACGACAGCG GGAGCAGCTC GGAGGGCAGC CTGCAGACCA CCCTGGAGGA CAGCCTGAAC 
CTGAGCGACT CCCCACAGCG TCCCTTGGAC CTCCCCGCCG CCCTCTTCCC GCGCCCGACG 
GCCGGCGCCC ACAGAACCGC GACGGCCCCA CCGTCCCCGG CCCCCCGGCG CCCGGGCCCT 
CGCACCCTCG GCCTCCGCGG GCTCCGGCGG CACCAACGCA GCCTCAGCAG CGGCGGGAGC 
ACCAGCCCCG GCTGCGCCCG CCGTGACTCC GTGGACCCTT CGGATGAGGA GGGGACGGGG 
GGCGGCCCCC GGGGGGGCGG CCCCGGCGGC GAACCCTCGG AGGCCCTGAG CACCCTCTCT 
CTGACTGCCC TCTTCTCGCC CCCGCCACCG CCCCCGGGCC TGGCCCTCAC CAAGAGATGC 
GGCAGCACCA GCAGCCTCTC CTGCTCCGCC CTGCCCTCCC GGGGCCTGGC GGCAGTGACC 
AGCAGACCGC CGTGCTCCGC CGGCCCCTAC GGCCTCCTCT CCGGGCCCGC CTGGGTGACG 
GACTTCTGCG AGGACCCCCC GGCCCCGAGC CAGGCGGCAG AGTGCGGCCC ACTGCCCGCT 
CCCAAGCTAG AGCCGGGACC AGGCCCGAGC AAGAGGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028934967.1
CDS	149..6733
Translation	MAELVSQPSSSSGWAPAAPPPAPSAPGPPDPADLGVTDQQPQAQPPPSPDLSGGAQPVPDPDVPHPDLAPVVFFCLKQTTSPRNWCIKMVCNPWFECVSMMVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLRQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFEKFRDGQEGRSDPKLCTIPMTPNGHLDPNLPLPSQPLAAGGVLPAPRNSLQPDQMLIALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCEKCNHDTQEVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESSLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_029079134.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029079134.1

ORF Insert Sequence:

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GAGAACTTGT GGCTGGACAG TGTCTCTTTA ATTATTAAGG ACTCCTTCGA AGGGGAGTTA 
ATGATCATCG ATAACCTTTC AGGCTCCGTC TTCCACCATT ACTCCTCGCC GGCCGTGTGC 
GAGAAGTGTA ACCATGACAC GCAAGAGGTC CAGCTGGCGG AGACGGAAGC TTTCTCCCTG 
AACTCGGACA AGTCCTCTTC CGTCCTGCTG GGGGACGACC TCAGCCTGGA TGACCCCGGC 
CTCTGCCCTC CCGGCGCCAA GGGGATCAAG GGCGAACCAG AGACCCCGGA CCCCGTCAAC 
GTGGGAGACT TGGGTGAATG TTTCTTCCCG CCGACTAACC TCGCAGTTCC TCCGCCTGCA 
GAGAGTTCTC TGGGGGAGAT GGAGACCACC CCCTTCCACC CCGTCCCTGC TACGTGGCTT 
GAGCAAGAGA GCAGCCCAGT TCCTTCGAGC CCTTTCTCTC CGGACACCCC CAGCCCCCTC 
TTGCCTGTGC CAGCCGAGTT CTTTCAGCCG GTCGTCTCCG CCAGCCAGCA GGGCCAGGAG 
AAAACTGCCC GCCCCGGGAG CCTCCCCAAG ATCTCCCTGC AGGGCTCCTG GGCGACCCTG 
CGCCCCCCAA CCGTGAACTG CACGCTTCTC CGTCAGCCCC CCGAGAGCGA CCCCTCCCTG 
GACGACAGCG GGAGCAGCTC GGAGGGCAGC CTGCAGACCA CCCTGGAGGA CAGCCTGAAC 
CTGAGCGACT CCCCACAGCG TCCCTTGGAC CTCCCCGCCG CCCTCTTCCC GCGCCCGACG 
GCCGGCGCCC ACAGAACCGC GACGGCCCCA CCGTCCCCGG CCCCCCGGCG CCCGGGCCCT 
CGCACCCTCG GCCTCCGCGG GCTCCGGCGG CACCAACGCA GCCTCAGCAG CGGCGGGAGC 
ACCAGCCCCG GCTGCGCCCG CCGTGACTCC GTGGACCCTT CGGATGAGGA GGGGACGGGG 
GGCGGCCCCC GGGGGGGCGG CCCCGGCGGC GAACCCTCGG AGGCCCTGAG CACCCTCTCT 
CTGACTGCCC TCTTCTCGCC CCCGCCACCG CCCCCGGGCC TGGCCCTCAC CAAGAGATGC 
GGCAGCACCA GCAGCCTCTC CTGCTCCGCC CTGCCCTCCC GGGGCCTGGC GGCAGTGACC 
AGCAGACCGC CGTGCTCCGC CGGCCCCTAC GGCCTCCTCT CCGGGCCCGC CTGGGTGACG 
GACTTCTGCG AGGACCCCCC GGCCCCGAGC CAGGCGGCAG AGTGCGGCCC ACTGCCCGCT 
CCCAAGCTAG AGCCGGGACC AGGCCCGAGC AAGAGGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_029079135.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028934968.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X2, mRNA.

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CloneID	OOc116263
Clone ID Related Accession (Same CDS sequence)	XM_029079134.1 , XM_029079135.1
Accession Version	XM_029079135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041741.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GAGAACTTGT GGCTGGACAG TGTCTCTTTA ATTATTAAGG ACTCCTTCGA AGGGGAGTTA 
ATGATCATCG ATAACCTTTC AGGCTCCGTC TTCCACCATT ACTCCTCGCC GGCCGTGTGC 
GAGAAGTGTA ACCATGACAC GCAAGAGGTC CAGCTGGCGG AGACGGAAGC TTTCTCCCTG 
AACTCGGACA AGTCCTCTTC CGTCCTGCTG GGGGACGACC TCAGCCTGGA TGACCCCGGC 
CTCTGCCCTC CCGGCGCCAA GGGGATCAAG GGCGAACCAG AGACCCCGGA CCCCGTCAAC 
GTGGGAGACT TGGGTGAATG TTTCTTCCCG CCGACTAACC TCGCAGTTCC TCCGCCTGCA 
GAGAGTTCTC TGGGGGAGAT GGAGACCACC CCCTTCCACC CCGTCCCTGC TACGTGGCTT 
GAGCAAGAGA GCAGCCCAGT TCCTTCGAGC CCTTTCTCTC CGGACACCCC CAGCCCCCTC 
TTGCCTGTGC CAGCCGAGTT CTTTCAGCCG GTCGTCTCCG CCAGCCAGCA GGGCCAGGAG 
AAAACTGCCC GCCCCGGGAG CCTCCCCAAG ATCTCCCTGC AGGGCTCCTG GGCGACCCTG 
CGCCCCCCAA CCGTGAACTG CACGCTTCTC CGTCAGCCCC CCGAGAGCGA CCCCTCCCTG 
GACGACAGCG GGAGCAGCTC GGAGGGCAGC CTGCAGACCA CCCTGGAGGA CAGCCTGAAC 
CTGAGCGACT CCCCACAGCG TCCCTTGGAC CTCCCCGCCG CCCTCTTCCC GCGCCCGACG 
GCCGGCGCCC ACAGAACCGC GACGGCCCCA CCGTCCCCGG CCCCCCGGCG CCCGGGCCCT 
CGCACCCTCG GCCTCCGCGG GCTCCGGCGG CACCAACGCA GCCTCAGCAG CGGCGGGAGC 
ACCAGCCCCG GCTGCGCCCG CCGTGACTCC GTGGACCCTT CGGATGAGGA GGGGACGGGG 
GGCGGCCCCC GGGGGGGCGG CCCCGGCGGC GAACCCTCGG AGGCCCTGAG CACCCTCTCT 
CTGACTGCCC TCTTCTCGCC CCCGCCACCG CCCCCGGGCC TGGCCCTCAC CAAGAGATGC 
GGCAGCACCA GCAGCCTCTC CTGCTCCGCC CTGCCCTCCC GGGGCCTGGC GGCAGTGACC 
AGCAGACCGC CGTGCTCCGC CGGCCCCTAC GGCCTCCTCT CCGGGCCCGC CTGGGTGACG 
GACTTCTGCG AGGACCCCCC GGCCCCGAGC CAGGCGGCAG AGTGCGGCCC ACTGCCCGCT 
CCCAAGCTAG AGCCGGGACC AGGCCCGAGC AAGAGGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028934968.1
CDS	87..6671
Translation	MAELVSQPSSSSGWAPAAPPPAPSAPGPPDPADLGVTDQQPQAQPPPSPDLSGGAQPVPDPDVPHPDLAPVVFFCLKQTTSPRNWCIKMVCNPWFECVSMMVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLRQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFEKFRDGQEGRSDPKLCTIPMTPNGHLDPNLPLPSQPLAAGGVLPAPRNSLQPDQMLIALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCEKCNHDTQEVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESSLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_029079135.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029079135.1

ORF Insert Sequence:

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GAGAACTTGT GGCTGGACAG TGTCTCTTTA ATTATTAAGG ACTCCTTCGA AGGGGAGTTA 
ATGATCATCG ATAACCTTTC AGGCTCCGTC TTCCACCATT ACTCCTCGCC GGCCGTGTGC 
GAGAAGTGTA ACCATGACAC GCAAGAGGTC CAGCTGGCGG AGACGGAAGC TTTCTCCCTG 
AACTCGGACA AGTCCTCTTC CGTCCTGCTG GGGGACGACC TCAGCCTGGA TGACCCCGGC 
CTCTGCCCTC CCGGCGCCAA GGGGATCAAG GGCGAACCAG AGACCCCGGA CCCCGTCAAC 
GTGGGAGACT TGGGTGAATG TTTCTTCCCG CCGACTAACC TCGCAGTTCC TCCGCCTGCA 
GAGAGTTCTC TGGGGGAGAT GGAGACCACC CCCTTCCACC CCGTCCCTGC TACGTGGCTT 
GAGCAAGAGA GCAGCCCAGT TCCTTCGAGC CCTTTCTCTC CGGACACCCC CAGCCCCCTC 
TTGCCTGTGC CAGCCGAGTT CTTTCAGCCG GTCGTCTCCG CCAGCCAGCA GGGCCAGGAG 
AAAACTGCCC GCCCCGGGAG CCTCCCCAAG ATCTCCCTGC AGGGCTCCTG GGCGACCCTG 
CGCCCCCCAA CCGTGAACTG CACGCTTCTC CGTCAGCCCC CCGAGAGCGA CCCCTCCCTG 
GACGACAGCG GGAGCAGCTC GGAGGGCAGC CTGCAGACCA CCCTGGAGGA CAGCCTGAAC 
CTGAGCGACT CCCCACAGCG TCCCTTGGAC CTCCCCGCCG CCCTCTTCCC GCGCCCGACG 
GCCGGCGCCC ACAGAACCGC GACGGCCCCA CCGTCCCCGG CCCCCCGGCG CCCGGGCCCT 
CGCACCCTCG GCCTCCGCGG GCTCCGGCGG CACCAACGCA GCCTCAGCAG CGGCGGGAGC 
ACCAGCCCCG GCTGCGCCCG CCGTGACTCC GTGGACCCTT CGGATGAGGA GGGGACGGGG 
GGCGGCCCCC GGGGGGGCGG CCCCGGCGGC GAACCCTCGG AGGCCCTGAG CACCCTCTCT 
CTGACTGCCC TCTTCTCGCC CCCGCCACCG CCCCCGGGCC TGGCCCTCAC CAAGAGATGC 
GGCAGCACCA GCAGCCTCTC CTGCTCCGCC CTGCCCTCCC GGGGCCTGGC GGCAGTGACC 
AGCAGACCGC CGTGCTCCGC CGGCCCCTAC GGCCTCCTCT CCGGGCCCGC CTGGGTGACG 
GACTTCTGCG AGGACCCCCC GGCCCCGAGC CAGGCGGCAG AGTGCGGCCC ACTGCCCGCT 
CCCAAGCTAG AGCCGGGACC AGGCCCGAGC AAGAGGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_029079136.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028934969.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X3, mRNA.

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CloneID	OOc116264
Clone ID Related Accession (Same CDS sequence)	XM_029079136.1
Accession Version	XM_029079136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041741.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTGCAGGGCG ACATCACCCT GCCCCCTTAC TACCAGCCCG AGGAGGACGA CGAGACGCCC 
TTCATCTGCT CGCTGTCGGG GGACAACGGC ATCATGGGGT GCCACGAGAT CCCGCCCCTC 
AAGGAGCGGG GCCACGAGTG CTGCCTGGAC AAGGACAGCG TGGCCTTAGA GCGGTCCGGC 
CGCCAAGACC ACAACGCCAG CGGCCTGTGC GTCAACTGGA ACCAGTATTA CAACGTGTGC 
CGCCCTGGCA GCGCCAACCC CCACAAGGGC GCCATCAACT TCGACAACAT CGGCTACGCC 
TGGATCGTCA TCTTCCAGGT GATCACCCTG GAAGGCTGGG TGGAGATCAT GTACTACGTG 
ATGGATGCTC ATTCCTTCTA CAATTTCATC TACTTTATCC TGCTCATTAT AGTGGGCTCC 
TTCTTCATGA TCAACCTGTG CCTCGTCGTC ATAGCGACCC AGTTCTCGGA GACCAAGCAG 
AGGGAGCACC GGCTGATGCA GGAGCAGAGG CAGCGGTACC TGTCGTCCAG CACGGTGGCC 
AGCTACACGG AGCCCGGCGA CTGCTACGAG GAGATCTTCC AGTACGTCTG TCACATCCTG 
CGCAAGGCCA AACGCCGCCT CCTGGGCCTC TACCACGCCC TGCAGACCCG CCGCCAGAGC 
CGCTGCCATC GGGGACTGGG CCGGGGCCAG CCGGAGCCCG GGGGCGACCC CCCGGGGGCC 
TGGGGAAACG GGAAACCACA GAAGCACTAT AAGCTTTGCC AGAAACACAA CCCCCTGGAC 
TACCCGCCGC ACACCCTCGT CCAGCCCATC TCGGTGACGC TGACCTCTGA CCCCGCCAAC 
TGCCCCAGAT GCCACCGGGC CCAGCGGGAG GCGGCCCGGC GGCTCTCCGG CCTGGACAGC 
CCGCCCTCGG ACCGGGAGGA GGGGTCCGGT GGCGGCCGGG GGGAGGCCAG CGGGAGCAGC 
GACGGCGCCT CAGAGCTGGA GAAGGAGGAG GAGGACGGGG ACCGAGGGGG CCTGAGGTGG 
CTGTGCGGGG CCGTGTGGAG GGAAATCAGG GCCAGGCTGC GCGGGATCGT CGACAGCAAG 
TACTTCAACC GCGGCATCAT GATCGCCATC CTGGTGAACA CCATCAGCAT GGGGATCGAG 
CACCACGAGC AGCCCGAGGA GCTGACCAAC ATCCTGGAAA TCAGCAACGT GGTGTTCACC 
AGCATGTTTG CGCTGGAGAT GATCCTGAAG CTGGCAGCCT TCGGCCTCTT CGACTACCTC 
CGCAACCCCT ACAACATCTT CGACAGCATC ATCGTCATCA TCAGCATCTG GGAGATCATC 
GGCCAGTCGG ACGGGGGCCT GTCGGTGCTG AGGACGTTCC GGCTGCTGCG CGTGCTGAAG 
CTGGTGCGGT TCATGCCCGC GCTGCGTCGC CAGCTGGTCG TGCTCATGAA GACCATGGAC 
AACGTGGCCA CCTTCTGCAT GCTGCTCATG CTCTTCATCT TCATCTTCAG CATCCTGGGG 
ATGCACATCT TCGGCTGCAA ATTCAGCCTC AGGACGGACA CGGGGGACAC GGTCCCGGAC 
CGGAAGAACT TCGACTCCCT GCTGTGGGCC ATCGTCACCG TGTTCCAGAT CCTGACGCAG 
GAGGACTGGA ATATGGTGCT GTACAACGGC ATGGCCTCCA CCTCGCCCTG GGCCTCCCTT 
TACTTTGTAG CCCTCATGAC CTTCGGCAAC TACGTGCTCT TCAATCTGCT GGTGGCCATC 
CTGGTGGAGG GCTTCCAGGC AGAGGGAGAC GCCAACCGCT CGTACTCGGA TGAGGACCAG 
AGCTCATCCA ACATGGAGGA GTTTGAGAAG TTCAGGGACG GCCAGGAGGG CCGCTCTGAT 
CCCAAACTCT GCACAATCCC CATGACCCCA AATGGACACC TGGACCCCAA CCTCCCGCTC 
CCCAGCCAGC CTCTGGCTGC GGGCGGGGTG CTGCCTGCCC CCCGGAATTC CCTGCAGCCC 
GACCAGATGC TCATCGCCCT AGGATCCCGG AAGAGCAGTG TGATGTCTCT GGGGAGGATG 
AGCTACGACC AGCGGTCCCT GTCCAGCTCG CGCAGCTCCT ACTATGGGGC TTGGGGCCGC 
AGCGCGGCCT GGGGGAGCCG CCGTGCCAGC TGGAACAGCC TGGCCCGGGG CCACAGCCTG 
AAGCACCAGC CCCCGTCCGC CGAGCACGAG TCCCTGCTGT CGGGGGAGAG GCGAGTGGTG 
CCTGACGGGG AGCGAGGCGG GGCCCGGGGG ACCCACCGCC ACCGCCGGAC GCTCTCCCTG 
GACACCAAGG ACCCCTCCGA CCTGGTGGAG GTGCCCCCTG CCCTCCCCCC AGCTCACCCC 
CGGGCCCCCC GCAAGGTGGG TGCCATCCCG GGGGCCGGGG AACACCAGAA CTGCAATGGC 
AAGATGCCCG GAGTTGCCAA GGACGTCTTC ACTAAGATGA ACGACCCGGA GGGCCACGGG 
GAGGACGAGG AAGAGATGGA TTACACCCTG TGTTTCCGCA TCCGGAAGAT GATGGACGTC 
TACAAGCCAG ACTGGTGTGA GATCCGTGAA GACTGGTCCA TCTACCTCTT CTCCCCTCAG 
AACAGGTTCC GGGTGCTCTG CCAGACCGTC ATCGCTCACA AACTCTTCGA CTACGTGGTC 
CTGGCCTTCA TCTTCCTCAA CTGCATCACC ATCGCCCTGG AGAGGCCTCA GATTGAGAAC 
CGCAGCACGG AGAGGATCTT CCTCACAGTC TCCAACTACA TCTTCACCGC CATCTTCGTG 
GCTGAGATGA CGCTCAAGGT GGTCTCGCTG GGCCTGTATT TCGGGGAGCA GGCCTATCTC 
CGCAGCAGCT GGAACATCCT GGACGGCTTC CTGGTCTTCG TGTCCATCAT CGACATCGTG 
GTCTCCATGG CCTCCGCGGG CGGCGCCAAG ATCCTGGGCG TCCTCCGTGT CCTCCGCCTC 
CTCCGCACCC TCCGCCCCCT CCGGGTGATC AGCCGGGCCC CGGGCCTGAA GCTGGTGGTG 
GAAACCCTGA TCTCCTCCCT GAAGCCCATC GGGAACATCG TCCTAATCTG CTGCGCCTTC 
TTCATCATCT TCGGCATCCT GGGAGTTCAG CTCTTCAAAG GGAAATTCTA CCACTGTCTG 
GGCCTGGACA TCCGCAACAT CACCAACCGC TCCGACTGCC TGGCCGCCAA CTACCGATGG 
GTGCACCACA AGTACAACTT CGACAACCTG GGCCAGGCCC TGATGTCCCT CTTCGTTCTG 
GCCTCGAAGG ACGGCTGGGT CAATATCATG TATAACGGAC TGGATGCGGT GGCTGTAGAC 
CAGCAGCCGG TGACCAACAA TAACCCCTGG ATGCTCCTGT ACTTCATCTC CTTCCTCCTC 
ATCGTCAGCT TCTTCGTGCT GAACATGTTC GTGGGTGTGG TGGTGGAGAA TTTCCACAAG 
TGCCGGCAGC ACCAGGAGGC CGAGGAGGCC CGGCGCCGTG AGGAGAAACG ACTACGGCGC 
CTCGACAAGA AGCGCAGGAA AGCCCAGCGG CTGCCGTACT ATGCAACCTA CTGCCCCACT 
CGCCTCCTCA TCCACTCTGT CTGTACCAGC CACTACCTGG ACATCTTCAT CACCTTCATC 
ATCTGCCTCA ACGTGGTCAC TATGTCCCTG GAGCACTACA ACCAGCCAGT GTCCCTGGAG 
ATCGCCCTCA AGTACTGCAA CTATATGTTC ACCACCGTGT TCGTGCTGGA GGCCGTGCTG 
AAGCTGGTGG CCTTTGGGCT GAGACGCTTC TTCAAAGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TACTGTCGGT CATGGGCATC ACCCTGGAGG AGATCGAGAT CAACGCCGCC 
CTGCCCATCA ACCCCACCAT CATCCGCATT ATGAGAGTCC TGAGGATCGC CCGCGTACTG 
AAGCTCCTGA AGATGGCCAC GGGGATGCGG GCTTTACTGG ACACGGTGGT GCAGGCCCTT 
CCTCAGGTGG GGAACCTTGG TCTGCTCTTC ATGCTCCTCT TCTTCATCTA CGCAGCCCTA 
GGAGTGGAGC TCTTTGGGAA ACTGGTGTGC AACGAGGAGA ACCCCTGTGA GGGCATGAGT 
CGCCACGCCA CCTTCGAGAA CTTCGGCATG GCCTTCTTGA CGCTCTTCCA GGTCTCCACT 
GGCGACAACT GGAATGGAAT CATGAAGGAT ACCCTCCGGG ATTGCACCCA CGACGAGCGC 
AGCTGCCTGA GCAACCTGCA GTTCATCTCG CCCCTGTACT TCGTCAGCTT CGTGCTCACG 
GCCCAGTTCG TCCTCATCAA CGTGGTCGTG GCCGTGCTCA TGAAGCACCT GGACGACAGC 
AACAAGGAGG CTCAGGAGGA TGCCGAGATG GACGCCGAGA TCGAGCTGGA GATGGCGCAC 
GGGCTGTGTC CCCGGGGGAC CAGCCCGCCG GGCCCCGGCA GGGGGGCAGG AGGAGGAGCC 
GGAGGAGCGG TCGACCCCGA GGGCCATCTC TGCATGAAGT GCTACTCTCC AGCCCAGGAG 
AACTTGTGGC TGGACAGTGT CTCTTTAATT ATTAAGGACT CCTTCGAAGG GGAGTTAATG 
ATCATCGATA ACCTTTCAGG CTCCGTCTTC CACCATTACT CCTCGCCGGC CGTGTGCGAG 
AAGTGTAACC ATGACACGCA AGAGGTCCAG CTGGCGGAGA CGGAAGCTTT CTCCCTGAAC 
TCGGACAAGT CCTCTTCCGT CCTGCTGGGG GACGACCTCA GCCTGGATGA CCCCGGCCTC 
TGCCCTCCCG GCGCCAAGGG GATCAAGGGC GAACCAGAGA CCCCGGACCC CGTCAACGTG 
GGAGACTTGG GTGAATGTTT CTTCCCGCCG ACTAACCTCG CAGTTCCTCC GCCTGCAGAG 
AGTTCTCTGG GGGAGATGGA GACCACCCCC TTCCACCCCG TCCCTGCTAC GTGGCTTGAG 
CAAGAGAGCA GCCCAGTTCC TTCGAGCCCT TTCTCTCCGG ACACCCCCAG CCCCCTCTTG 
CCTGTGCCAG CCGAGTTCTT TCAGCCGGTC GTCTCCGCCA GCCAGCAGGG CCAGGAGAAA 
ACTGCCCGCC CCGGGAGCCT CCCCAAGATC TCCCTGCAGG GCTCCTGGGC GACCCTGCGC 
CCCCCAACCG TGAACTGCAC GCTTCTCCGT CAGCCCCCCG AGAGCGACCC CTCCCTGGAC 
GACAGCGGGA GCAGCTCGGA GGGCAGCCTG CAGACCACCC TGGAGGACAG CCTGAACCTG 
AGCGACTCCC CACAGCGTCC CTTGGACCTC CCCGCCGCCC TCTTCCCGCG CCCGACGGCC 
GGCGCCCACA GAACCGCGAC GGCCCCACCG TCCCCGGCCC CCCGGCGCCC GGGCCCTCGC 
ACCCTCGGCC TCCGCGGGCT CCGGCGGCAC CAACGCAGCC TCAGCAGCGG CGGGAGCACC 
AGCCCCGGCT GCGCCCGCCG TGACTCCGTG GACCCTTCGG ATGAGGAGGG GACGGGGGGC 
GGCCCCCGGG GGGGCGGCCC CGGCGGCGAA CCCTCGGAGG CCCTGAGCAC CCTCTCTCTG 
ACTGCCCTCT TCTCGCCCCC GCCACCGCCC CCGGGCCTGG CCCTCACCAA GAGATGCGGC 
AGCACCAGCA GCCTCTCCTG CTCCGCCCTG CCCTCCCGGG GCCTGGCGGC AGTGACCAGC 
AGACCGCCGT GCTCCGCCGG CCCCTACGGC CTCCTCTCCG GGCCCGCCTG GGTGACGGAC 
TTCTGCGAGG ACCCCCCGGC CCCGAGCCAG GCGGCAGAGT GCGGCCCACT GCCCGCTCCC 
AAGCTAGAGC CGGGACCAGG CCCGAGCAAG AGGAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028934969.1
CDS	149..6730
Translation	MAELVSQPSSSSGWAPAAPPPAPSAPGPPDPADLGVTDQQPQAQPPPSPDLSGGAQPVPDPDVPHPDLAPVVFFCLKQTTSPRNWCIKMVCNPWFECVSMMVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFEKFRDGQEGRSDPKLCTIPMTPNGHLDPNLPLPSQPLAAGGVLPAPRNSLQPDQMLIALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCEKCNHDTQEVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESSLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_029079136.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029079136.1

ORF Insert Sequence:

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTGCAGGGCG ACATCACCCT GCCCCCTTAC TACCAGCCCG AGGAGGACGA CGAGACGCCC 
TTCATCTGCT CGCTGTCGGG GGACAACGGC ATCATGGGGT GCCACGAGAT CCCGCCCCTC 
AAGGAGCGGG GCCACGAGTG CTGCCTGGAC AAGGACAGCG TGGCCTTAGA GCGGTCCGGC 
CGCCAAGACC ACAACGCCAG CGGCCTGTGC GTCAACTGGA ACCAGTATTA CAACGTGTGC 
CGCCCTGGCA GCGCCAACCC CCACAAGGGC GCCATCAACT TCGACAACAT CGGCTACGCC 
TGGATCGTCA TCTTCCAGGT GATCACCCTG GAAGGCTGGG TGGAGATCAT GTACTACGTG 
ATGGATGCTC ATTCCTTCTA CAATTTCATC TACTTTATCC TGCTCATTAT AGTGGGCTCC 
TTCTTCATGA TCAACCTGTG CCTCGTCGTC ATAGCGACCC AGTTCTCGGA GACCAAGCAG 
AGGGAGCACC GGCTGATGCA GGAGCAGAGG CAGCGGTACC TGTCGTCCAG CACGGTGGCC 
AGCTACACGG AGCCCGGCGA CTGCTACGAG GAGATCTTCC AGTACGTCTG TCACATCCTG 
CGCAAGGCCA AACGCCGCCT CCTGGGCCTC TACCACGCCC TGCAGACCCG CCGCCAGAGC 
CGCTGCCATC GGGGACTGGG CCGGGGCCAG CCGGAGCCCG GGGGCGACCC CCCGGGGGCC 
TGGGGAAACG GGAAACCACA GAAGCACTAT AAGCTTTGCC AGAAACACAA CCCCCTGGAC 
TACCCGCCGC ACACCCTCGT CCAGCCCATC TCGGTGACGC TGACCTCTGA CCCCGCCAAC 
TGCCCCAGAT GCCACCGGGC CCAGCGGGAG GCGGCCCGGC GGCTCTCCGG CCTGGACAGC 
CCGCCCTCGG ACCGGGAGGA GGGGTCCGGT GGCGGCCGGG GGGAGGCCAG CGGGAGCAGC 
GACGGCGCCT CAGAGCTGGA GAAGGAGGAG GAGGACGGGG ACCGAGGGGG CCTGAGGTGG 
CTGTGCGGGG CCGTGTGGAG GGAAATCAGG GCCAGGCTGC GCGGGATCGT CGACAGCAAG 
TACTTCAACC GCGGCATCAT GATCGCCATC CTGGTGAACA CCATCAGCAT GGGGATCGAG 
CACCACGAGC AGCCCGAGGA GCTGACCAAC ATCCTGGAAA TCAGCAACGT GGTGTTCACC 
AGCATGTTTG CGCTGGAGAT GATCCTGAAG CTGGCAGCCT TCGGCCTCTT CGACTACCTC 
CGCAACCCCT ACAACATCTT CGACAGCATC ATCGTCATCA TCAGCATCTG GGAGATCATC 
GGCCAGTCGG ACGGGGGCCT GTCGGTGCTG AGGACGTTCC GGCTGCTGCG CGTGCTGAAG 
CTGGTGCGGT TCATGCCCGC GCTGCGTCGC CAGCTGGTCG TGCTCATGAA GACCATGGAC 
AACGTGGCCA CCTTCTGCAT GCTGCTCATG CTCTTCATCT TCATCTTCAG CATCCTGGGG 
ATGCACATCT TCGGCTGCAA ATTCAGCCTC AGGACGGACA CGGGGGACAC GGTCCCGGAC 
CGGAAGAACT TCGACTCCCT GCTGTGGGCC ATCGTCACCG TGTTCCAGAT CCTGACGCAG 
GAGGACTGGA ATATGGTGCT GTACAACGGC ATGGCCTCCA CCTCGCCCTG GGCCTCCCTT 
TACTTTGTAG CCCTCATGAC CTTCGGCAAC TACGTGCTCT TCAATCTGCT GGTGGCCATC 
CTGGTGGAGG GCTTCCAGGC AGAGGGAGAC GCCAACCGCT CGTACTCGGA TGAGGACCAG 
AGCTCATCCA ACATGGAGGA GTTTGAGAAG TTCAGGGACG GCCAGGAGGG CCGCTCTGAT 
CCCAAACTCT GCACAATCCC CATGACCCCA AATGGACACC TGGACCCCAA CCTCCCGCTC 
CCCAGCCAGC CTCTGGCTGC GGGCGGGGTG CTGCCTGCCC CCCGGAATTC CCTGCAGCCC 
GACCAGATGC TCATCGCCCT AGGATCCCGG AAGAGCAGTG TGATGTCTCT GGGGAGGATG 
AGCTACGACC AGCGGTCCCT GTCCAGCTCG CGCAGCTCCT ACTATGGGGC TTGGGGCCGC 
AGCGCGGCCT GGGGGAGCCG CCGTGCCAGC TGGAACAGCC TGGCCCGGGG CCACAGCCTG 
AAGCACCAGC CCCCGTCCGC CGAGCACGAG TCCCTGCTGT CGGGGGAGAG GCGAGTGGTG 
CCTGACGGGG AGCGAGGCGG GGCCCGGGGG ACCCACCGCC ACCGCCGGAC GCTCTCCCTG 
GACACCAAGG ACCCCTCCGA CCTGGTGGAG GTGCCCCCTG CCCTCCCCCC AGCTCACCCC 
CGGGCCCCCC GCAAGGTGGG TGCCATCCCG GGGGCCGGGG AACACCAGAA CTGCAATGGC 
AAGATGCCCG GAGTTGCCAA GGACGTCTTC ACTAAGATGA ACGACCCGGA GGGCCACGGG 
GAGGACGAGG AAGAGATGGA TTACACCCTG TGTTTCCGCA TCCGGAAGAT GATGGACGTC 
TACAAGCCAG ACTGGTGTGA GATCCGTGAA GACTGGTCCA TCTACCTCTT CTCCCCTCAG 
AACAGGTTCC GGGTGCTCTG CCAGACCGTC ATCGCTCACA AACTCTTCGA CTACGTGGTC 
CTGGCCTTCA TCTTCCTCAA CTGCATCACC ATCGCCCTGG AGAGGCCTCA GATTGAGAAC 
CGCAGCACGG AGAGGATCTT CCTCACAGTC TCCAACTACA TCTTCACCGC CATCTTCGTG 
GCTGAGATGA CGCTCAAGGT GGTCTCGCTG GGCCTGTATT TCGGGGAGCA GGCCTATCTC 
CGCAGCAGCT GGAACATCCT GGACGGCTTC CTGGTCTTCG TGTCCATCAT CGACATCGTG 
GTCTCCATGG CCTCCGCGGG CGGCGCCAAG ATCCTGGGCG TCCTCCGTGT CCTCCGCCTC 
CTCCGCACCC TCCGCCCCCT CCGGGTGATC AGCCGGGCCC CGGGCCTGAA GCTGGTGGTG 
GAAACCCTGA TCTCCTCCCT GAAGCCCATC GGGAACATCG TCCTAATCTG CTGCGCCTTC 
TTCATCATCT TCGGCATCCT GGGAGTTCAG CTCTTCAAAG GGAAATTCTA CCACTGTCTG 
GGCCTGGACA TCCGCAACAT CACCAACCGC TCCGACTGCC TGGCCGCCAA CTACCGATGG 
GTGCACCACA AGTACAACTT CGACAACCTG GGCCAGGCCC TGATGTCCCT CTTCGTTCTG 
GCCTCGAAGG ACGGCTGGGT CAATATCATG TATAACGGAC TGGATGCGGT GGCTGTAGAC 
CAGCAGCCGG TGACCAACAA TAACCCCTGG ATGCTCCTGT ACTTCATCTC CTTCCTCCTC 
ATCGTCAGCT TCTTCGTGCT GAACATGTTC GTGGGTGTGG TGGTGGAGAA TTTCCACAAG 
TGCCGGCAGC ACCAGGAGGC CGAGGAGGCC CGGCGCCGTG AGGAGAAACG ACTACGGCGC 
CTCGACAAGA AGCGCAGGAA AGCCCAGCGG CTGCCGTACT ATGCAACCTA CTGCCCCACT 
CGCCTCCTCA TCCACTCTGT CTGTACCAGC CACTACCTGG ACATCTTCAT CACCTTCATC 
ATCTGCCTCA ACGTGGTCAC TATGTCCCTG GAGCACTACA ACCAGCCAGT GTCCCTGGAG 
ATCGCCCTCA AGTACTGCAA CTATATGTTC ACCACCGTGT TCGTGCTGGA GGCCGTGCTG 
AAGCTGGTGG CCTTTGGGCT GAGACGCTTC TTCAAAGACA GGTGGAACCA GCTGGACCTG 
GCCATCGTGC TACTGTCGGT CATGGGCATC ACCCTGGAGG AGATCGAGAT CAACGCCGCC 
CTGCCCATCA ACCCCACCAT CATCCGCATT ATGAGAGTCC TGAGGATCGC CCGCGTACTG 
AAGCTCCTGA AGATGGCCAC GGGGATGCGG GCTTTACTGG ACACGGTGGT GCAGGCCCTT 
CCTCAGGTGG GGAACCTTGG TCTGCTCTTC ATGCTCCTCT TCTTCATCTA CGCAGCCCTA 
GGAGTGGAGC TCTTTGGGAA ACTGGTGTGC AACGAGGAGA ACCCCTGTGA GGGCATGAGT 
CGCCACGCCA CCTTCGAGAA CTTCGGCATG GCCTTCTTGA CGCTCTTCCA GGTCTCCACT 
GGCGACAACT GGAATGGAAT CATGAAGGAT ACCCTCCGGG ATTGCACCCA CGACGAGCGC 
AGCTGCCTGA GCAACCTGCA GTTCATCTCG CCCCTGTACT TCGTCAGCTT CGTGCTCACG 
GCCCAGTTCG TCCTCATCAA CGTGGTCGTG GCCGTGCTCA TGAAGCACCT GGACGACAGC 
AACAAGGAGG CTCAGGAGGA TGCCGAGATG GACGCCGAGA TCGAGCTGGA GATGGCGCAC 
GGGCTGTGTC CCCGGGGGAC CAGCCCGCCG GGCCCCGGCA GGGGGGCAGG AGGAGGAGCC 
GGAGGAGCGG TCGACCCCGA GGGCCATCTC TGCATGAAGT GCTACTCTCC AGCCCAGGAG 
AACTTGTGGC TGGACAGTGT CTCTTTAATT ATTAAGGACT CCTTCGAAGG GGAGTTAATG 
ATCATCGATA ACCTTTCAGG CTCCGTCTTC CACCATTACT CCTCGCCGGC CGTGTGCGAG 
AAGTGTAACC ATGACACGCA AGAGGTCCAG CTGGCGGAGA CGGAAGCTTT CTCCCTGAAC 
TCGGACAAGT CCTCTTCCGT CCTGCTGGGG GACGACCTCA GCCTGGATGA CCCCGGCCTC 
TGCCCTCCCG GCGCCAAGGG GATCAAGGGC GAACCAGAGA CCCCGGACCC CGTCAACGTG 
GGAGACTTGG GTGAATGTTT CTTCCCGCCG ACTAACCTCG CAGTTCCTCC GCCTGCAGAG 
AGTTCTCTGG GGGAGATGGA GACCACCCCC TTCCACCCCG TCCCTGCTAC GTGGCTTGAG 
CAAGAGAGCA GCCCAGTTCC TTCGAGCCCT TTCTCTCCGG ACACCCCCAG CCCCCTCTTG 
CCTGTGCCAG CCGAGTTCTT TCAGCCGGTC GTCTCCGCCA GCCAGCAGGG CCAGGAGAAA 
ACTGCCCGCC CCGGGAGCCT CCCCAAGATC TCCCTGCAGG GCTCCTGGGC GACCCTGCGC 
CCCCCAACCG TGAACTGCAC GCTTCTCCGT CAGCCCCCCG AGAGCGACCC CTCCCTGGAC 
GACAGCGGGA GCAGCTCGGA GGGCAGCCTG CAGACCACCC TGGAGGACAG CCTGAACCTG 
AGCGACTCCC CACAGCGTCC CTTGGACCTC CCCGCCGCCC TCTTCCCGCG CCCGACGGCC 
GGCGCCCACA GAACCGCGAC GGCCCCACCG TCCCCGGCCC CCCGGCGCCC GGGCCCTCGC 
ACCCTCGGCC TCCGCGGGCT CCGGCGGCAC CAACGCAGCC TCAGCAGCGG CGGGAGCACC 
AGCCCCGGCT GCGCCCGCCG TGACTCCGTG GACCCTTCGG ATGAGGAGGG GACGGGGGGC 
GGCCCCCGGG GGGGCGGCCC CGGCGGCGAA CCCTCGGAGG CCCTGAGCAC CCTCTCTCTG 
ACTGCCCTCT TCTCGCCCCC GCCACCGCCC CCGGGCCTGG CCCTCACCAA GAGATGCGGC 
AGCACCAGCA GCCTCTCCTG CTCCGCCCTG CCCTCCCGGG GCCTGGCGGC AGTGACCAGC 
AGACCGCCGT GCTCCGCCGG CCCCTACGGC CTCCTCTCCG GGCCCGCCTG GGTGACGGAC 
TTCTGCGAGG ACCCCCCGGC CCCGAGCCAG GCGGCAGAGT GCGGCCCACT GCCCGCTCCC 
AAGCTAGAGC CGGGACCAGG CCCGAGCAAG AGGAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_029079137.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028934970.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X4, mRNA.

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CloneID	OOc116265
Clone ID Related Accession (Same CDS sequence)	XM_029079137.1
Accession Version	XM_029079137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041741.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GGATCCCGGA AGAGCAGTGT GATGTCTCTG GGGAGGATGA GCTACGACCA GCGGTCCCTG 
TCCAGCTCGC GCAGCTCCTA CTATGGGGCT TGGGGCCGCA GCGCGGCCTG GGGGAGCCGC 
CGTGCCAGCT GGAACAGCCT GGCCCGGGGC CACAGCCTGA AGCACCAGCC CCCGTCCGCC 
GAGCACGAGT CCCTGCTGTC GGGGGAGAGG CGAGTGGTGC CTGACGGGGA GCGAGGCGGG 
GCCCGGGGGA CCCACCGCCA CCGCCGGACG CTCTCCCTGG ACACCAAGGA CCCCTCCGAC 
CTGGTGGAGG TGCCCCCTGC CCTCCCCCCA GCTCACCCCC GGGCCCCCCG CAAGGTGGGT 
GCCATCCCGG GGGCCGGGGA ACACCAGAAC TGCAATGGCA AGATGCCCGG AGTTGCCAAG 
GACGTCTTCA CTAAGATGAA CGACCCGGAG GGCCACGGGG AGGACGAGGA AGAGATGGAT 
TACACCCTGT GTTTCCGCAT CCGGAAGATG ATGGACGTCT ACAAGCCAGA CTGGTGTGAG 
ATCCGTGAAG ACTGGTCCAT CTACCTCTTC TCCCCTCAGA ACAGGTTCCG GGTGCTCTGC 
CAGACCGTCA TCGCTCACAA ACTCTTCGAC TACGTGGTCC TGGCCTTCAT CTTCCTCAAC 
TGCATCACCA TCGCCCTGGA GAGGCCTCAG ATTGAGAACC GCAGCACGGA GAGGATCTTC 
CTCACAGTCT CCAACTACAT CTTCACCGCC ATCTTCGTGG CTGAGATGAC GCTCAAGGTG 
GTCTCGCTGG GCCTGTATTT CGGGGAGCAG GCCTATCTCC GCAGCAGCTG GAACATCCTG 
GACGGCTTCC TGGTCTTCGT GTCCATCATC GACATCGTGG TCTCCATGGC CTCCGCGGGC 
GGCGCCAAGA TCCTGGGCGT CCTCCGTGTC CTCCGCCTCC TCCGCACCCT CCGCCCCCTC 
CGGGTGATCA GCCGGGCCCC GGGCCTGAAG CTGGTGGTGG AAACCCTGAT CTCCTCCCTG 
AAGCCCATCG GGAACATCGT CCTAATCTGC TGCGCCTTCT TCATCATCTT CGGCATCCTG 
GGAGTTCAGC TCTTCAAAGG GAAATTCTAC CACTGTCTGG GCCTGGACAT CCGCAACATC 
ACCAACCGCT CCGACTGCCT GGCCGCCAAC TACCGATGGG TGCACCACAA GTACAACTTC 
GACAACCTGG GCCAGGCCCT GATGTCCCTC TTCGTTCTGG CCTCGAAGGA CGGCTGGGTC 
AATATCATGT ATAACGGACT GGATGCGGTG GCTGTAGACC AGCAGCCGGT GACCAACAAT 
AACCCCTGGA TGCTCCTGTA CTTCATCTCC TTCCTCCTCA TCGTCAGCTT CTTCGTGCTG 
AACATGTTCG TGGGTGTGGT GGTGGAGAAT TTCCACAAGT GCCGGCAGCA CCAGGAGGCC 
GAGGAGGCCC GGCGCCGTGA GGAGAAACGA CTACGGCGCC TCGACAAGAA GCGCAGGAAA 
GCCCAGCGGC TGCCGTACTA TGCAACCTAC TGCCCCACTC GCCTCCTCAT CCACTCTGTC 
TGTACCAGCC ACTACCTGGA CATCTTCATC ACCTTCATCA TCTGCCTCAA CGTGGTCACT 
ATGTCCCTGG AGCACTACAA CCAGCCAGTG TCCCTGGAGA TCGCCCTCAA GTACTGCAAC 
TATATGTTCA CCACCGTGTT CGTGCTGGAG GCCGTGCTGA AGCTGGTGGC CTTTGGGCTG 
AGACGCTTCT TCAAAGACAG GTGGAACCAG CTGGACCTGG CCATCGTGCT ACTGTCGGTC 
ATGGGCATCA CCCTGGAGGA GATCGAGATC AACGCCGCCC TGCCCATCAA CCCCACCATC 
ATCCGCATTA TGAGAGTCCT GAGGATCGCC CGCGTACTGA AGCTCCTGAA GATGGCCACG 
GGGATGCGGG CTTTACTGGA CACGGTGGTG CAGGCCCTTC CTCAGGTGGG GAACCTTGGT 
CTGCTCTTCA TGCTCCTCTT CTTCATCTAC GCAGCCCTAG GAGTGGAGCT CTTTGGGAAA 
CTGGTGTGCA ACGAGGAGAA CCCCTGTGAG GGCATGAGTC GCCACGCCAC CTTCGAGAAC 
TTCGGCATGG CCTTCTTGAC GCTCTTCCAG GTCTCCACTG GCGACAACTG GAATGGAATC 
ATGAAGGATA CCCTCCGGGA TTGCACCCAC GACGAGCGCA GCTGCCTGAG CAACCTGCAG 
TTCATCTCGC CCCTGTACTT CGTCAGCTTC GTGCTCACGG CCCAGTTCGT CCTCATCAAC 
GTGGTCGTGG CCGTGCTCAT GAAGCACCTG GACGACAGCA ACAAGGAGGC TCAGGAGGAT 
GCCGAGATGG ACGCCGAGAT CGAGCTGGAG ATGGCGCACG GGCTGTGTCC CCGGGGGACC 
AGCCCGCCGG GCCCCGGCAG GGGGGCAGGA GGAGGAGCCG GAGGAGCGGT CGACCCCGAG 
GGCCATCTCT GCATGAAGTG CTACTCTCCA GCCCAGGAGA ACTTGTGGCT GGACAGTGTC 
TCTTTAATTA TTAAGGACTC CTTCGAAGGG GAGTTAATGA TCATCGATAA CCTTTCAGGC 
TCCGTCTTCC ACCATTACTC CTCGCCGGCC GTGTGCGAGA AGTGTAACCA TGACACGCAA 
GAGGTCCAGC TGGCGGAGAC GGAAGCTTTC TCCCTGAACT CGGACAAGTC CTCTTCCGTC 
CTGCTGGGGG ACGACCTCAG CCTGGATGAC CCCGGCCTCT GCCCTCCCGG CGCCAAGGGG 
ATCAAGGGCG AACCAGAGAC CCCGGACCCC GTCAACGTGG GAGACTTGGG TGAATGTTTC 
TTCCCGCCGA CTAACCTCGC AGTTCCTCCG CCTGCAGAGA GTTCTCTGGG GGAGATGGAG 
ACCACCCCCT TCCACCCCGT CCCTGCTACG TGGCTTGAGC AAGAGAGCAG CCCAGTTCCT 
TCGAGCCCTT TCTCTCCGGA CACCCCCAGC CCCCTCTTGC CTGTGCCAGC CGAGTTCTTT 
CAGCCGGTCG TCTCCGCCAG CCAGCAGGGC CAGGAGAAAA CTGCCCGCCC CGGGAGCCTC 
CCCAAGATCT CCCTGCAGGG CTCCTGGGCG ACCCTGCGCC CCCCAACCGT GAACTGCACG 
CTTCTCCGTC AGCCCCCCGA GAGCGACCCC TCCCTGGACG ACAGCGGGAG CAGCTCGGAG 
GGCAGCCTGC AGACCACCCT GGAGGACAGC CTGAACCTGA GCGACTCCCC ACAGCGTCCC 
TTGGACCTCC CCGCCGCCCT CTTCCCGCGC CCGACGGCCG GCGCCCACAG AACCGCGACG 
GCCCCACCGT CCCCGGCCCC CCGGCGCCCG GGCCCTCGCA CCCTCGGCCT CCGCGGGCTC 
CGGCGGCACC AACGCAGCCT CAGCAGCGGC GGGAGCACCA GCCCCGGCTG CGCCCGCCGT 
GACTCCGTGG ACCCTTCGGA TGAGGAGGGG ACGGGGGGCG GCCCCCGGGG GGGCGGCCCC 
GGCGGCGAAC CCTCGGAGGC CCTGAGCACC CTCTCTCTGA CTGCCCTCTT CTCGCCCCCG 
CCACCGCCCC CGGGCCTGGC CCTCACCAAG AGATGCGGCA GCACCAGCAG CCTCTCCTGC 
TCCGCCCTGC CCTCCCGGGG CCTGGCGGCA GTGACCAGCA GACCGCCGTG CTCCGCCGGC 
CCCTACGGCC TCCTCTCCGG GCCCGCCTGG GTGACGGACT TCTGCGAGGA CCCCCCGGCC 
CCGAGCCAGG CGGCAGAGTG CGGCCCACTG CCCGCTCCCA AGCTAGAGCC GGGACCAGGC 
CCGAGCAAGA GGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028934970.1
CDS	149..6589
Translation	MAELVSQPSSSSGWAPAAPPPAPSAPGPPDPADLGVTDQQPQAQPPPSPDLSGGAQPVPDPDVPHPDLAPVVFFCLKQTTSPRNWCIKMVCNPWFECVSMMVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLRQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFEKFRDGQEGRSGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCEKCNHDTQEVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESSLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_029079137.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029079137.1

ORF Insert Sequence:

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GGATCCCGGA AGAGCAGTGT GATGTCTCTG GGGAGGATGA GCTACGACCA GCGGTCCCTG 
TCCAGCTCGC GCAGCTCCTA CTATGGGGCT TGGGGCCGCA GCGCGGCCTG GGGGAGCCGC 
CGTGCCAGCT GGAACAGCCT GGCCCGGGGC CACAGCCTGA AGCACCAGCC CCCGTCCGCC 
GAGCACGAGT CCCTGCTGTC GGGGGAGAGG CGAGTGGTGC CTGACGGGGA GCGAGGCGGG 
GCCCGGGGGA CCCACCGCCA CCGCCGGACG CTCTCCCTGG ACACCAAGGA CCCCTCCGAC 
CTGGTGGAGG TGCCCCCTGC CCTCCCCCCA GCTCACCCCC GGGCCCCCCG CAAGGTGGGT 
GCCATCCCGG GGGCCGGGGA ACACCAGAAC TGCAATGGCA AGATGCCCGG AGTTGCCAAG 
GACGTCTTCA CTAAGATGAA CGACCCGGAG GGCCACGGGG AGGACGAGGA AGAGATGGAT 
TACACCCTGT GTTTCCGCAT CCGGAAGATG ATGGACGTCT ACAAGCCAGA CTGGTGTGAG 
ATCCGTGAAG ACTGGTCCAT CTACCTCTTC TCCCCTCAGA ACAGGTTCCG GGTGCTCTGC 
CAGACCGTCA TCGCTCACAA ACTCTTCGAC TACGTGGTCC TGGCCTTCAT CTTCCTCAAC 
TGCATCACCA TCGCCCTGGA GAGGCCTCAG ATTGAGAACC GCAGCACGGA GAGGATCTTC 
CTCACAGTCT CCAACTACAT CTTCACCGCC ATCTTCGTGG CTGAGATGAC GCTCAAGGTG 
GTCTCGCTGG GCCTGTATTT CGGGGAGCAG GCCTATCTCC GCAGCAGCTG GAACATCCTG 
GACGGCTTCC TGGTCTTCGT GTCCATCATC GACATCGTGG TCTCCATGGC CTCCGCGGGC 
GGCGCCAAGA TCCTGGGCGT CCTCCGTGTC CTCCGCCTCC TCCGCACCCT CCGCCCCCTC 
CGGGTGATCA GCCGGGCCCC GGGCCTGAAG CTGGTGGTGG AAACCCTGAT CTCCTCCCTG 
AAGCCCATCG GGAACATCGT CCTAATCTGC TGCGCCTTCT TCATCATCTT CGGCATCCTG 
GGAGTTCAGC TCTTCAAAGG GAAATTCTAC CACTGTCTGG GCCTGGACAT CCGCAACATC 
ACCAACCGCT CCGACTGCCT GGCCGCCAAC TACCGATGGG TGCACCACAA GTACAACTTC 
GACAACCTGG GCCAGGCCCT GATGTCCCTC TTCGTTCTGG CCTCGAAGGA CGGCTGGGTC 
AATATCATGT ATAACGGACT GGATGCGGTG GCTGTAGACC AGCAGCCGGT GACCAACAAT 
AACCCCTGGA TGCTCCTGTA CTTCATCTCC TTCCTCCTCA TCGTCAGCTT CTTCGTGCTG 
AACATGTTCG TGGGTGTGGT GGTGGAGAAT TTCCACAAGT GCCGGCAGCA CCAGGAGGCC 
GAGGAGGCCC GGCGCCGTGA GGAGAAACGA CTACGGCGCC TCGACAAGAA GCGCAGGAAA 
GCCCAGCGGC TGCCGTACTA TGCAACCTAC TGCCCCACTC GCCTCCTCAT CCACTCTGTC 
TGTACCAGCC ACTACCTGGA CATCTTCATC ACCTTCATCA TCTGCCTCAA CGTGGTCACT 
ATGTCCCTGG AGCACTACAA CCAGCCAGTG TCCCTGGAGA TCGCCCTCAA GTACTGCAAC 
TATATGTTCA CCACCGTGTT CGTGCTGGAG GCCGTGCTGA AGCTGGTGGC CTTTGGGCTG 
AGACGCTTCT TCAAAGACAG GTGGAACCAG CTGGACCTGG CCATCGTGCT ACTGTCGGTC 
ATGGGCATCA CCCTGGAGGA GATCGAGATC AACGCCGCCC TGCCCATCAA CCCCACCATC 
ATCCGCATTA TGAGAGTCCT GAGGATCGCC CGCGTACTGA AGCTCCTGAA GATGGCCACG 
GGGATGCGGG CTTTACTGGA CACGGTGGTG CAGGCCCTTC CTCAGGTGGG GAACCTTGGT 
CTGCTCTTCA TGCTCCTCTT CTTCATCTAC GCAGCCCTAG GAGTGGAGCT CTTTGGGAAA 
CTGGTGTGCA ACGAGGAGAA CCCCTGTGAG GGCATGAGTC GCCACGCCAC CTTCGAGAAC 
TTCGGCATGG CCTTCTTGAC GCTCTTCCAG GTCTCCACTG GCGACAACTG GAATGGAATC 
ATGAAGGATA CCCTCCGGGA TTGCACCCAC GACGAGCGCA GCTGCCTGAG CAACCTGCAG 
TTCATCTCGC CCCTGTACTT CGTCAGCTTC GTGCTCACGG CCCAGTTCGT CCTCATCAAC 
GTGGTCGTGG CCGTGCTCAT GAAGCACCTG GACGACAGCA ACAAGGAGGC TCAGGAGGAT 
GCCGAGATGG ACGCCGAGAT CGAGCTGGAG ATGGCGCACG GGCTGTGTCC CCGGGGGACC 
AGCCCGCCGG GCCCCGGCAG GGGGGCAGGA GGAGGAGCCG GAGGAGCGGT CGACCCCGAG 
GGCCATCTCT GCATGAAGTG CTACTCTCCA GCCCAGGAGA ACTTGTGGCT GGACAGTGTC 
TCTTTAATTA TTAAGGACTC CTTCGAAGGG GAGTTAATGA TCATCGATAA CCTTTCAGGC 
TCCGTCTTCC ACCATTACTC CTCGCCGGCC GTGTGCGAGA AGTGTAACCA TGACACGCAA 
GAGGTCCAGC TGGCGGAGAC GGAAGCTTTC TCCCTGAACT CGGACAAGTC CTCTTCCGTC 
CTGCTGGGGG ACGACCTCAG CCTGGATGAC CCCGGCCTCT GCCCTCCCGG CGCCAAGGGG 
ATCAAGGGCG AACCAGAGAC CCCGGACCCC GTCAACGTGG GAGACTTGGG TGAATGTTTC 
TTCCCGCCGA CTAACCTCGC AGTTCCTCCG CCTGCAGAGA GTTCTCTGGG GGAGATGGAG 
ACCACCCCCT TCCACCCCGT CCCTGCTACG TGGCTTGAGC AAGAGAGCAG CCCAGTTCCT 
TCGAGCCCTT TCTCTCCGGA CACCCCCAGC CCCCTCTTGC CTGTGCCAGC CGAGTTCTTT 
CAGCCGGTCG TCTCCGCCAG CCAGCAGGGC CAGGAGAAAA CTGCCCGCCC CGGGAGCCTC 
CCCAAGATCT CCCTGCAGGG CTCCTGGGCG ACCCTGCGCC CCCCAACCGT GAACTGCACG 
CTTCTCCGTC AGCCCCCCGA GAGCGACCCC TCCCTGGACG ACAGCGGGAG CAGCTCGGAG 
GGCAGCCTGC AGACCACCCT GGAGGACAGC CTGAACCTGA GCGACTCCCC ACAGCGTCCC 
TTGGACCTCC CCGCCGCCCT CTTCCCGCGC CCGACGGCCG GCGCCCACAG AACCGCGACG 
GCCCCACCGT CCCCGGCCCC CCGGCGCCCG GGCCCTCGCA CCCTCGGCCT CCGCGGGCTC 
CGGCGGCACC AACGCAGCCT CAGCAGCGGC GGGAGCACCA GCCCCGGCTG CGCCCGCCGT 
GACTCCGTGG ACCCTTCGGA TGAGGAGGGG ACGGGGGGCG GCCCCCGGGG GGGCGGCCCC 
GGCGGCGAAC CCTCGGAGGC CCTGAGCACC CTCTCTCTGA CTGCCCTCTT CTCGCCCCCG 
CCACCGCCCC CGGGCCTGGC CCTCACCAAG AGATGCGGCA GCACCAGCAG CCTCTCCTGC 
TCCGCCCTGC CCTCCCGGGG CCTGGCGGCA GTGACCAGCA GACCGCCGTG CTCCGCCGGC 
CCCTACGGCC TCCTCTCCGG GCCCGCCTGG GTGACGGACT TCTGCGAGGA CCCCCCGGCC 
CCGAGCCAGG CGGCAGAGTG CGGCCCACTG CCCGCTCCCA AGCTAGAGCC GGGACCAGGC 
CCGAGCAAGA GGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_029079138.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028934971.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X5, mRNA.

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CloneID	OOc116266
Clone ID Related Accession (Same CDS sequence)	XM_029079138.1
Accession Version	XM_029079138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041741.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GTCCAGCTGG CGGAGACGGA AGCTTTCTCC CTGAACTCGG ACAAGTCCTC TTCCGTCCTG 
CTGGGGGACG ACCTCAGCCT GGATGACCCC GGCCTCTGCC CTCCCGGCGC CAAGGGGATC 
AAGGGCGAAC CAGAGACCCC GGACCCCGTC AACGTGGGAG ACTTGGGTGA ATGTTTCTTC 
CCGCCGACTA ACCTCGCAGT TCCTCCGCCT GCAGAGAGTT CTCTGGGGGA GATGGAGACC 
ACCCCCTTCC ACCCCGTCCC TGCTACGTGG CTTGAGCAAG AGAGCAGCCC AGTTCCTTCG 
AGCCCTTTCT CTCCGGACAC CCCCAGCCCC CTCTTGCCTG TGCCAGCCGA GTTCTTTCAG 
CCGGTCGTCT CCGCCAGCCA GCAGGGCCAG GAGAAAACTG CCCGCCCCGG GAGCCTCCCC 
AAGATCTCCC TGCAGGGCTC CTGGGCGACC CTGCGCCCCC CAACCGTGAA CTGCACGCTT 
CTCCGTCAGC CCCCCGAGAG CGACCCCTCC CTGGACGACA GCGGGAGCAG CTCGGAGGGC 
AGCCTGCAGA CCACCCTGGA GGACAGCCTG AACCTGAGCG ACTCCCCACA GCGTCCCTTG 
GACCTCCCCG CCGCCCTCTT CCCGCGCCCG ACGGCCGGCG CCCACAGAAC CGCGACGGCC 
CCACCGTCCC CGGCCCCCCG GCGCCCGGGC CCTCGCACCC TCGGCCTCCG CGGGCTCCGG 
CGGCACCAAC GCAGCCTCAG CAGCGGCGGG AGCACCAGCC CCGGCTGCGC CCGCCGTGAC 
TCCGTGGACC CTTCGGATGA GGAGGGGACG GGGGGCGGCC CCCGGGGGGG CGGCCCCGGC 
GGCGAACCCT CGGAGGCCCT GAGCACCCTC TCTCTGACTG CCCTCTTCTC GCCCCCGCCA 
CCGCCCCCGG GCCTGGCCCT CACCAAGAGA TGCGGCAGCA CCAGCAGCCT CTCCTGCTCC 
GCCCTGCCCT CCCGGGGCCT GGCGGCAGTG ACCAGCAGAC CGCCGTGCTC CGCCGGCCCC 
TACGGCCTCC TCTCCGGGCC CGCCTGGGTG ACGGACTTCT GCGAGGACCC CCCGGCCCCG 
AGCCAGGCGG CAGAGTGCGG CCCACTGCCC GCTCCCAAGC TAGAGCCGGG ACCAGGCCCG 
AGCAAGAGGA ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028934971.1
CDS	149..6586
Translation	MAELVSQPSSSSGWAPAAPPPAPSAPGPPDPADLGVTDQQPQAQPPPSPDLSGGAQPVPDPDVPHPDLAPVVFFCLKQTTSPRNWCIKMVCNPWFECVSMMVILLNCVTLGMYQPCEDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLRQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFEKFRDGQEGRSDPKLCTIPMTPNGHLDPNLPLPSQPLAAGGVLPAPRNSLQPDQMLIALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESSLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_029079138.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029079138.1

ORF Insert Sequence:

ATGGCCGAAC TGGTCTCCCA GCCCTCTTCC TCTTCGGGCT GGGCTCCTGC CGCCCCACCC 
CCTGCGCCCT CAGCCCCAGG CCCCCCGGAC CCCGCTGACC TCGGAGTTAC GGACCAGCAG 
CCTCAGGCGC AGCCCCCGCC GAGCCCGGAC CTGAGCGGCG GCGCCCAACC CGTGCCCGAC 
CCGGACGTCC CTCACCCCGA CCTGGCTCCG GTGGTGTTCT TCTGCCTCAA ACAGACCACC 
AGCCCCCGCA ACTGGTGCAT CAAGATGGTG TGTAACCCTT GGTTCGAGTG CGTCAGCATG 
ATGGTGATTC TCCTGAACTG TGTGACCCTG GGGATGTACC AGCCCTGTGA GGATATGGAC 
TGCCTCTCTG ACCGCTGTAA GATCCTACAG GTGTTCGATG ACTTCATCTT CATCTTCTTT 
GCCATGGAGA TGGTGCTGAA GATGGTGGCC CTGGGCATCT TCGGGAAGAA GTGCTACCTC 
GGGGACACCT GGAACCGTCT GGATTTCTTC ATCGTCATGG CAGGGATGGT GGAATACTCC 
CTGGACCTTC AGAACATCAA CCTGTCGGCG ATCCGCACAG TCCGGGTCCT GAGGCCCCTC 
AAAGCCATCA ACCGGGTGCC CAGCATGCGG ATCCTGGTGA ACCTACTCCT GGACACGCTG 
CCCATGCTGG GAAACGTCCT GCTGCTCTGC TTCTTCGTCT TCTTCATCTT CGGCATCATC 
GGCGTGCAGC TGTGGGCCGG CCTGCTCCGC AACCGCTGCT TCCTGGAGGA CAACTTCACC 
CTCAGGCAGG GCGACATCAC CCTGCCCCCT TACTACCAGC CCGAGGAGGA CGACGAGACG 
CCCTTCATCT GCTCGCTGTC GGGGGACAAC GGCATCATGG GGTGCCACGA GATCCCGCCC 
CTCAAGGAGC GGGGCCACGA GTGCTGCCTG GACAAGGACA GCGTGGCCTT AGAGCGGTCC 
GGCCGCCAAG ACCACAACGC CAGCGGCCTG TGCGTCAACT GGAACCAGTA TTACAACGTG 
TGCCGCCCTG GCAGCGCCAA CCCCCACAAG GGCGCCATCA ACTTCGACAA CATCGGCTAC 
GCCTGGATCG TCATCTTCCA GGTGATCACC CTGGAAGGCT GGGTGGAGAT CATGTACTAC 
GTGATGGATG CTCATTCCTT CTACAATTTC ATCTACTTTA TCCTGCTCAT TATAGTGGGC 
TCCTTCTTCA TGATCAACCT GTGCCTCGTC GTCATAGCGA CCCAGTTCTC GGAGACCAAG 
CAGAGGGAGC ACCGGCTGAT GCAGGAGCAG AGGCAGCGGT ACCTGTCGTC CAGCACGGTG 
GCCAGCTACA CGGAGCCCGG CGACTGCTAC GAGGAGATCT TCCAGTACGT CTGTCACATC 
CTGCGCAAGG CCAAACGCCG CCTCCTGGGC CTCTACCACG CCCTGCAGAC CCGCCGCCAG 
AGCCGCTGCC ATCGGGGACT GGGCCGGGGC CAGCCGGAGC CCGGGGGCGA CCCCCCGGGG 
GCCTGGGGAA ACGGGAAACC ACAGAAGCAC TATAAGCTTT GCCAGAAACA CAACCCCCTG 
GACTACCCGC CGCACACCCT CGTCCAGCCC ATCTCGGTGA CGCTGACCTC TGACCCCGCC 
AACTGCCCCA GATGCCACCG GGCCCAGCGG GAGGCGGCCC GGCGGCTCTC CGGCCTGGAC 
AGCCCGCCCT CGGACCGGGA GGAGGGGTCC GGTGGCGGCC GGGGGGAGGC CAGCGGGAGC 
AGCGACGGCG CCTCAGAGCT GGAGAAGGAG GAGGAGGACG GGGACCGAGG GGGCCTGAGG 
TGGCTGTGCG GGGCCGTGTG GAGGGAAATC AGGGCCAGGC TGCGCGGGAT CGTCGACAGC 
AAGTACTTCA ACCGCGGCAT CATGATCGCC ATCCTGGTGA ACACCATCAG CATGGGGATC 
GAGCACCACG AGCAGCCCGA GGAGCTGACC AACATCCTGG AAATCAGCAA CGTGGTGTTC 
ACCAGCATGT TTGCGCTGGA GATGATCCTG AAGCTGGCAG CCTTCGGCCT CTTCGACTAC 
CTCCGCAACC CCTACAACAT CTTCGACAGC ATCATCGTCA TCATCAGCAT CTGGGAGATC 
ATCGGCCAGT CGGACGGGGG CCTGTCGGTG CTGAGGACGT TCCGGCTGCT GCGCGTGCTG 
AAGCTGGTGC GGTTCATGCC CGCGCTGCGT CGCCAGCTGG TCGTGCTCAT GAAGACCATG 
GACAACGTGG CCACCTTCTG CATGCTGCTC ATGCTCTTCA TCTTCATCTT CAGCATCCTG 
GGGATGCACA TCTTCGGCTG CAAATTCAGC CTCAGGACGG ACACGGGGGA CACGGTCCCG 
GACCGGAAGA ACTTCGACTC CCTGCTGTGG GCCATCGTCA CCGTGTTCCA GATCCTGACG 
CAGGAGGACT GGAATATGGT GCTGTACAAC GGCATGGCCT CCACCTCGCC CTGGGCCTCC 
CTTTACTTTG TAGCCCTCAT GACCTTCGGC AACTACGTGC TCTTCAATCT GCTGGTGGCC 
ATCCTGGTGG AGGGCTTCCA GGCAGAGGGA GACGCCAACC GCTCGTACTC GGATGAGGAC 
CAGAGCTCAT CCAACATGGA GGAGTTTGAG AAGTTCAGGG ACGGCCAGGA GGGCCGCTCT 
GATCCCAAAC TCTGCACAAT CCCCATGACC CCAAATGGAC ACCTGGACCC CAACCTCCCG 
CTCCCCAGCC AGCCTCTGGC TGCGGGCGGG GTGCTGCCTG CCCCCCGGAA TTCCCTGCAG 
CCCGACCAGA TGCTCATCGC CCTAGGATCC CGGAAGAGCA GTGTGATGTC TCTGGGGAGG 
ATGAGCTACG ACCAGCGGTC CCTGTCCAGC TCGCGCAGCT CCTACTATGG GGCTTGGGGC 
CGCAGCGCGG CCTGGGGGAG CCGCCGTGCC AGCTGGAACA GCCTGGCCCG GGGCCACAGC 
CTGAAGCACC AGCCCCCGTC CGCCGAGCAC GAGTCCCTGC TGTCGGGGGA GAGGCGAGTG 
GTGCCTGACG GGGAGCGAGG CGGGGCCCGG GGGACCCACC GCCACCGCCG GACGCTCTCC 
CTGGACACCA AGGACCCCTC CGACCTGGTG GAGGTGCCCC CTGCCCTCCC CCCAGCTCAC 
CCCCGGGCCC CCCGCAAGGT GGGTGCCATC CCGGGGGCCG GGGAACACCA GAACTGCAAT 
GGCAAGATGC CCGGAGTTGC CAAGGACGTC TTCACTAAGA TGAACGACCC GGAGGGCCAC 
GGGGAGGACG AGGAAGAGAT GGATTACACC CTGTGTTTCC GCATCCGGAA GATGATGGAC 
GTCTACAAGC CAGACTGGTG TGAGATCCGT GAAGACTGGT CCATCTACCT CTTCTCCCCT 
CAGAACAGGT TCCGGGTGCT CTGCCAGACC GTCATCGCTC ACAAACTCTT CGACTACGTG 
GTCCTGGCCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC TCAGATTGAG 
AACCGCAGCA CGGAGAGGAT CTTCCTCACA GTCTCCAACT ACATCTTCAC CGCCATCTTC 
GTGGCTGAGA TGACGCTCAA GGTGGTCTCG CTGGGCCTGT ATTTCGGGGA GCAGGCCTAT 
CTCCGCAGCA GCTGGAACAT CCTGGACGGC TTCCTGGTCT TCGTGTCCAT CATCGACATC 
GTGGTCTCCA TGGCCTCCGC GGGCGGCGCC AAGATCCTGG GCGTCCTCCG TGTCCTCCGC 
CTCCTCCGCA CCCTCCGCCC CCTCCGGGTG ATCAGCCGGG CCCCGGGCCT GAAGCTGGTG 
GTGGAAACCC TGATCTCCTC CCTGAAGCCC ATCGGGAACA TCGTCCTAAT CTGCTGCGCC 
TTCTTCATCA TCTTCGGCAT CCTGGGAGTT CAGCTCTTCA AAGGGAAATT CTACCACTGT 
CTGGGCCTGG ACATCCGCAA CATCACCAAC CGCTCCGACT GCCTGGCCGC CAACTACCGA 
TGGGTGCACC ACAAGTACAA CTTCGACAAC CTGGGCCAGG CCCTGATGTC CCTCTTCGTT 
CTGGCCTCGA AGGACGGCTG GGTCAATATC ATGTATAACG GACTGGATGC GGTGGCTGTA 
GACCAGCAGC CGGTGACCAA CAATAACCCC TGGATGCTCC TGTACTTCAT CTCCTTCCTC 
CTCATCGTCA GCTTCTTCGT GCTGAACATG TTCGTGGGTG TGGTGGTGGA GAATTTCCAC 
AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCCCGGCGCC GTGAGGAGAA ACGACTACGG 
CGCCTCGACA AGAAGCGCAG GAAAGCCCAG CGGCTGCCGT ACTATGCAAC CTACTGCCCC 
ACTCGCCTCC TCATCCACTC TGTCTGTACC AGCCACTACC TGGACATCTT CATCACCTTC 
ATCATCTGCC TCAACGTGGT CACTATGTCC CTGGAGCACT ACAACCAGCC AGTGTCCCTG 
GAGATCGCCC TCAAGTACTG CAACTATATG TTCACCACCG TGTTCGTGCT GGAGGCCGTG 
CTGAAGCTGG TGGCCTTTGG GCTGAGACGC TTCTTCAAAG ACAGGTGGAA CCAGCTGGAC 
CTGGCCATCG TGCTACTGTC GGTCATGGGC ATCACCCTGG AGGAGATCGA GATCAACGCC 
GCCCTGCCCA TCAACCCCAC CATCATCCGC ATTATGAGAG TCCTGAGGAT CGCCCGCGTA 
CTGAAGCTCC TGAAGATGGC CACGGGGATG CGGGCTTTAC TGGACACGGT GGTGCAGGCC 
CTTCCTCAGG TGGGGAACCT TGGTCTGCTC TTCATGCTCC TCTTCTTCAT CTACGCAGCC 
CTAGGAGTGG AGCTCTTTGG GAAACTGGTG TGCAACGAGG AGAACCCCTG TGAGGGCATG 
AGTCGCCACG CCACCTTCGA GAACTTCGGC ATGGCCTTCT TGACGCTCTT CCAGGTCTCC 
ACTGGCGACA ACTGGAATGG AATCATGAAG GATACCCTCC GGGATTGCAC CCACGACGAG 
CGCAGCTGCC TGAGCAACCT GCAGTTCATC TCGCCCCTGT ACTTCGTCAG CTTCGTGCTC 
ACGGCCCAGT TCGTCCTCAT CAACGTGGTC GTGGCCGTGC TCATGAAGCA CCTGGACGAC 
AGCAACAAGG AGGCTCAGGA GGATGCCGAG ATGGACGCCG AGATCGAGCT GGAGATGGCG 
CACGGGCTGT GTCCCCGGGG GACCAGCCCG CCGGGCCCCG GCAGGGGGGC AGGAGGAGGA 
GCCGGAGGAG CGGTCGACCC CGAGGGCCAT CTCTGCATGA AGTGCTACTC TCCAGCCCAG 
GTCCAGCTGG CGGAGACGGA AGCTTTCTCC CTGAACTCGG ACAAGTCCTC TTCCGTCCTG 
CTGGGGGACG ACCTCAGCCT GGATGACCCC GGCCTCTGCC CTCCCGGCGC CAAGGGGATC 
AAGGGCGAAC CAGAGACCCC GGACCCCGTC AACGTGGGAG ACTTGGGTGA ATGTTTCTTC 
CCGCCGACTA ACCTCGCAGT TCCTCCGCCT GCAGAGAGTT CTCTGGGGGA GATGGAGACC 
ACCCCCTTCC ACCCCGTCCC TGCTACGTGG CTTGAGCAAG AGAGCAGCCC AGTTCCTTCG 
AGCCCTTTCT CTCCGGACAC CCCCAGCCCC CTCTTGCCTG TGCCAGCCGA GTTCTTTCAG 
CCGGTCGTCT CCGCCAGCCA GCAGGGCCAG GAGAAAACTG CCCGCCCCGG GAGCCTCCCC 
AAGATCTCCC TGCAGGGCTC CTGGGCGACC CTGCGCCCCC CAACCGTGAA CTGCACGCTT 
CTCCGTCAGC CCCCCGAGAG CGACCCCTCC CTGGACGACA GCGGGAGCAG CTCGGAGGGC 
AGCCTGCAGA CCACCCTGGA GGACAGCCTG AACCTGAGCG ACTCCCCACA GCGTCCCTTG 
GACCTCCCCG CCGCCCTCTT CCCGCGCCCG ACGGCCGGCG CCCACAGAAC CGCGACGGCC 
CCACCGTCCC CGGCCCCCCG GCGCCCGGGC CCTCGCACCC TCGGCCTCCG CGGGCTCCGG 
CGGCACCAAC GCAGCCTCAG CAGCGGCGGG AGCACCAGCC CCGGCTGCGC CCGCCGTGAC 
TCCGTGGACC CTTCGGATGA GGAGGGGACG GGGGGCGGCC CCCGGGGGGG CGGCCCCGGC 
GGCGAACCCT CGGAGGCCCT GAGCACCCTC TCTCTGACTG CCCTCTTCTC GCCCCCGCCA 
CCGCCCCCGG GCCTGGCCCT CACCAAGAGA TGCGGCAGCA CCAGCAGCCT CTCCTGCTCC 
GCCCTGCCCT CCCGGGGCCT GGCGGCAGTG ACCAGCAGAC CGCCGTGCTC CGCCGGCCCC 
TACGGCCTCC TCTCCGGGCC CGCCTGGGTG ACGGACTTCT GCGAGGACCC CCCGGCCCCG 
AGCCAGGCGG CAGAGTGCGG CCCACTGCCC GCTCCCAAGC TAGAGCCGGG ACCAGGCCCG 
AGCAAGAGGA ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_003431046.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_003431094.1 Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

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CloneID	OOc12700
Clone ID Related Accession (Same CDS sequence)	XM_003431046.2
Accession Version	XM_003431046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794386.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 29, 2014 this sequence version replaced XM_003431046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGTGGAAT ACTCCCTGGA CCTTCAGAAC ATCAACCTGT CGGCGATCCG CACAGTCCGG 
GTCCTGAGGC CCCTCAAAGC CATCAACCGG GTGCCCAGCA TGCGGATCCT GGTGAACCTA 
CTCCTGGACA CGCTGCCCAT GCTGGGAAAC GTCCTGCTGC TCTGCTTCTT CGTCTTCTTC 
ATCTTCGGCA TCATCGGCGT GCAGCTGTGG GCCGGCCTGC TCCGCAACCG CTGCTTCCTG 
GAGGACAACT TCACCCTGCA GGGCGACATC ACCCTGCCCC CTTACTACCA GCCCGAGGAG 
GACGACGAGA CGCCCTTCAT CTGCTCGCTG TCGGGGGACA ACGGCATCAT GGGGTGCCAC 
GAGATCCCGC CCCTCAAGGA GCGGGGCCAC GAGTGCTGCC TGGACAAGGA CAGCGTGGCC 
TTAGAGCGGT CCGGCCGCCA AGACCACAAC GCCAGCGGCC TGTGCGTCAA CTGGAACCAG 
TATTACAACG TGTGCCGCCC CGGCAGCGCC AACCCCCACA AGGGCGCCAT CAACTTCGAC 
AACATCGGCT ACGCCTGGAT CGTCATCTTC CAGGTGATCA CCCTGGAAGG CTGGGTGGAG 
ATCATGTACT ACGTGATGGA TGCTCATTCC TTCTACAATT TCATCTACTT TATCCTGCTC 
ATTATAGTGG GCTCCTTCTT CATGATCAAC CTGTGCCTCG TCGTCATAGC GACCCAGTTC 
TCGGAGACCA AGCAGAGGGA GCACCGGCTG ATGCAGGAGC AGAGGCAGCG GTACCTGTCG 
TCCAGCACGG TGGCCAGCTA CACGGAGCCC GGCGACTGCT ACGAGGAGAT CTTCCAGTAC 
GTCTGTCACA TCCTGCGCAA GGCCAAACGC CGCCTCCTGG GCCTCTACCA CGCCCTGCAG 
ACCCGCCGCC AGAGCCGCTG CCATCGGGGA CTGGGCCGGG GCCAGCCGGA GCCCGGGGGC 
GACCCCCCGG GGGCCTGGGG AAACGGGAAA CCACAGAAGC ACTATAAGCT TTGCCAGAAA 
CACAACCCCC TGGACTACCC GCCGCACACC CTCGTCCAGC CCATCTCGGT GACGCTGACC 
TCTGACCCCG CCAACTGCCC CAGATGCCAC CGGGCCCAGC GGGAGGCGGC CCGGCGGCTC 
TCCGGCCTGG ACAGCCCGCC CTCGGACCGG GAGGAGGGGT CCGGTGGCGG CCGGGGGGAG 
GCCAGCGGGA GCAGCGACGG CGCCTCAGAG CTGGAGAAGG AGGAGGAGGA CGGGGACCGA 
GGGGGCCTGA GGTGGCTGTG CGGGGCCGTG TGGAGGGAAA TCAGGGCCAG GCTGCGCGGG 
ATCGTCGACA GCAAGTACTT CAACCGCGGC ATCATGATCG CCATCCTGGT GAACACCATC 
AGCATGGGGA TCGAGCACCA CGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAAATCAGC 
AACGTGGTGT TCACCAGCAT GTTTGCGCTG GAGATGATCC TGAAGCTGGC AGCCTTCGGC 
CTCTTCGACT ACCTCCGCAA CCCCTACAAC ATCTTCGACA GCATCATCGT CATCATCAGC 
ATCTGGGAGA TCATCGGCCA GTCGGACGGG GGCCTGTCGG TGCTGAGGAC GTTCCGGCTG 
CTGCGCGTGC TGAAGCTGGT GCGGTTCATG CCCGCGCTGC GTCGCCAGCT GGTCGTGCTC 
ATGAAGACCA TGGACAACGT GGCCACCTTC TGCATGCTGC TCATGCTCTT CATCTTCATC 
TTCAGGACCT ATCCCAAACT CTGCACAATC CCCATGACCC CAAATGGACA CCTGGACCCC 
AACCTCCCGC TCCCCAGCCA GCCTCTGGCT GCGGGCGGGG TGCTGCCTGC CCCCCGGAAT 
TCCCTGCAGC CCGACCAGAT GCTCATCGCC CTAGGATCCC GGAAGAGCAG TGTGATGTCT 
CTGGGGAGGA TGAGCTACGA CCAGCGGTCC CTGTCCAGCT CGCGCAGCTC CTACTATGGG 
GCTTGGGGCC GCAGCGCGGC CTGGGGGAGC CGCCGTGCCA GCTGGAACAG CCTGGCCCGG 
GGCCACAGCC TGAAGCACCA GCCCCCGTCC GCCGAGCACG AGTCCCTGCT GTCGGGGGAG 
AGGCGAGTGG TGCCTGACGG GGAGCGAGGC GGGGCCCGGG GGACCCACCG CCACCGCCGG 
ACGCTCTCCC TGGACACCAA GGACCCCTCC GACCTGGTGG AGGTGCCCCC TGCCCTCCCC 
CCAGCTCACC CCCGGGCCCC CCGCAAGGTG GGTGCCATCC CGGGGGCCGG GGAACACCAG 
AACTGCAATG GCAAGATGCC CGGAGTTGCC AAGGACGTCT TCACTAAGAT GAACGACCCG 
GAGGGCCACG GGGAGGACGA GGAAGAGATG GATTACACCC TGTGTTTCCG CATCCGGAAG 
ATGATGGACG TCTACAAGCC AGACTGGTGT GAGATCCGTG AAGACTGGTC CATCTACCTC 
TTCTCCCCTC AGAACAGGTT CCGGGTGCTC TGCCAGACCG TCATCGCTCA CAAACTCTTC 
GACTACGTGG TCCTGGCCTT CATCTTCCTC AACTGCATCA CCATCGCCCT GGAGAGGCCT 
CAGATTGAGA ACCGCAGCAC GGAGAGGATC TTCCTCACAG TCTCCAACTA CATCTTCACC 
GCCATCTTCG TGGCTGAGAT GACGCTCAAG GTGGTCTCGC TGGGCCTGTA TTTCGGGGAG 
CAGGCCTATC TCCGCAGCAG CTGGAACATC CTGGACGGCT TCCTGGTCTT CGTGTCCATC 
ATCGACATCG TGGTCTCCAT GGCCTCCGCG GGCGGCGCCA AGATCCTGGG CGTCCTCCGT 
GTCCTCCGCC TCCTCCGCAC CCTCCGCCCC CTCCGGGTGA TCAGCCGGGC CCCGGGCCTG 
AAGCTGGTGG TGGAAACCCT GATCTCCTCC CTGAAGCCCA TCGGGAACAT CGTCCTAATC 
TGCTGCGCCT TCTTCATCAT CTTCGGCATC CTGGGAGTTC AGCTCTTCAA AGGGAAATTC 
TACCACTGTC TGGGCCTGGA CATCCGCAAC ATCACCAACC GCTCCGACTG CCTGGCCGCC 
AACTACCGAT GGGTGCACCA CAAGTACAAC TTCGACAACC TGGGCCAGGC CCTGATGTCC 
CTCTTCGTTC TGGCCTCGAA GGACGGCTGG GTCAATATCA TGTATAACGG ACTGGATGCG 
GTGGCTGTAG ACCAGCAGCC GGTGACCAAC AATAACCCCT GGATGCTCCT GTACTTCATC 
TCCTTCCTCC TCATCGTCAG CTTCTTCGTG CTGAACATGT TCGTGGGTGT GGTGGTGGAG 
AATTTCCACA AGTGCCGGCA GCACCAGGAG GCCGAGGAGG CCCGGCGCCG TGAGGAGAAA 
CGACTACGGC GCCTCGACAA GAAGCGCAGG AAAGCCCAGC GGCTGCCGTA CTATGCAACC 
TACTGCCCCA CTCGCCTCCT CATCCACTCT GTCTGTACCA GCCACTACCT GGACATCTTC 
ATCACCTTCA TCATCTGCCT CAACGTGGTC ACTATGTCCC TGGAGCACTA CAACCAGCCA 
GTGTCCCTGG AGATCGCCCT CAAGTACTGC AACTATATGT TCACCACCGT GTTCGTGCTG 
GAGGCCGTGC TGAAGCTGGT GGCCTTTGGG CTGAGACGCT TCTTCAAAGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTACTGTCG GTCATGGGCA TCACCCTGGA GGAGATCGAG 
ATCAACGCCG CCCTGCCCAT CAACCCCACC ATCATCCGCA TTATGAGAGT CCTGAGGATC 
GCCCGCGTAC TGAAGCTCCT GAAGATGGCC ACGGGGATGC GGGCTTTACT GGACACGGTG 
GTGCAGGCCC TTCCTCAGGT GGGGAACCTT GGTCTGCTCT TCATGCTCCT CTTCTTCATC 
TACGCAGCCC TAGGAGTGGA GCTCTTTGGG AAACTGGTGT GCAACGAGGA GAACCCCTGT 
GAGGGCATGA GTCGCCACGC CACCTTCGAG AACTTCGGCA TGGCCTTCTT GACGCTCTTC 
CAGGTCTCCA CTGGCGACAA CTGGAATGGA ATCATGAAGG ATACCCTCCG GGATTGCACC 
CACGACGAGC GCAGCTGCCT GAGCAACCTG CAGTTCATCT CGCCCCTGTA CTTCGTCAGC 
TTCGTGCTCA CGGCCCAGTT CGTCCTCATC AACGTGGTCG TGGCCGTGCT CATGAAGCAC 
CTGGACGACA GCAACAAGGA GGCTCAGGAG GATGCCGAGA TGGACGCCGA GATCGAGCTG 
GAGATGGCGC ACGGGCTGTG TCCCCGGGGG ACCAGCCCGC CGGGCCCCGG CAGGGGGGCA 
GGAGGAGGAG CCGGAGGAGC GGTCGACCCC GAGGGCCATC TCTGCATGAA GTGCTACTCT 
CCAGCCCAGG AGAACTTGTG GCTGGACAGT GTCTCTTTAA TTATTAAGGA CTCCTTCGAA 
GGGGAGTTAA TGATCATCGA TAACCTTTCA GGCTCCGTCT TCCACCATTA CTCCTCGCCG 
GCCGTGTGCG AGAAGTGTAA CCATGACACG CAAGAGGTCC AGCTGGCGGA GACGGAAGCT 
TTCTCCCTGA ACTCGGACAA GTCCTCTTCC GTCCTGCTGG GGGACGACCT CAGCCTGGAT 
GACCCCGGCC TCTGCCCTCC CGGCGCCAAG GGGATCAAGG GCGAACCAGA GACCCCGGAC 
CCCGTCAACG TGGGAGACTT GGGTGAATGT TTCTTCCCGC CGACTAACCT CGCAGTTCCT 
CCGCCTGCAG AGAGTTATCT GGGGGAGATG GAGACCACCC CCTTCCACCC CGTCCCTGCT 
ACGTGGCTTG AGCAAGAGAG CAGCCCAGTT CCTTCGAGCC CTTTCTCTCC GGACACCCCC 
AGCCCCCTCT TGCCTGTGCC AGCCGAGTTC TTTCAGCCGG TCGTCTCCGC CAGCCAGCAG 
GGCCAGGAGA AAACTGCCCG CCCCGGGAGC CTCCCCAAGA TCTCCCTGCA GGGCTCCTGG 
GCGACCCTGC GCCCCCCAAC CGTGAACTGC ACGCTTCTCC GTCAGCCCCC CGAGAGCGAC 
CCCTCCCTGG ACGACAGCGG GAGCAGCTCG GAGGGCAGCC TGCAGACCAC CCTGGAGGAC 
AGCCTGAACC TGAGCGACTC CCCACAGCGT CCCTTGGACC TCCCCGCCGC CCTCTTCCCG 
CGCCCGACGG CCGGCGCCCA CAGAACCGCG ACGGCCCCAC CGTCCCCGGC CCCCCGGCGC 
CCGGGCCCTC GCACCCTCGG CCTCCGCGGG CTCCGGCGGC ACCAACGCAG CCTCAGCAGC 
GGCGGGAGCA CCAGCCCCGG CTGCGCCCGC CGTGACTCCG TGGACCCTTC GGATGAGGAG 
GGGACGGGGG GCGGCCCCCG GGGGGGCGGC CCCGGCGGCG AACCCTCGGA GGCCCTGAGC 
ACCCTCTCTC TGACTGCCCT CTTCTCGCCC CCGCCACCGC CCCCGGGCCT GGCCCTCACC 
AAGAGATGCG GCAGCACCAG CAGCCTCTCC TGCTCCGCCC TGCCCTCCCG GGGCCTGGCG 
GCAGTGACCA GCAGACCGCC GTGCTCCGCC GGCCCCTACG GCCTCCTCTC CGGGCCCGCC 
TGGGTGACGG ACTTCTGCGA GGACCCCCCG GCCCCGAGCC AGGCGGCAGA GTGCGGCCCA 
CTGCCCGCTC CCAAGCTAGA GCCGGGACCA GGCCCGAGCA AGAGGAACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431094.1
CDS	1..5694
Translation	MVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEDNFTLQGDITLPPYYQPEEDDETPFICSLSGDNGIMGCHEIPPLKERGHECCLDKDSVALERSGRQDHNASGLCVNWNQYYNVCRPGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMQEQRQRYLSSSTVASYTEPGDCYEEIFQYVCHILRKAKRRLLGLYHALQTRRQSRCHRGLGRGQPEPGGDPPGAWGNGKPQKHYKLCQKHNPLDYPPHTLVQPISVTLTSDPANCPRCHRAQREAARRLSGLDSPPSDREEGSGGGRGEASGSSDGASELEKEEEDGDRGGLRWLCGAVWREIRARLRGIVDSKYFNRGIMIAILVNTISMGIEHHEQPEELTNILEISNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIIGQSDGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFRTYPKLCTIPMTPNGHLDPNLPLPSQPLAAGGVLPAPRNSLQPDQMLIALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGAWGRSAAWGSRRASWNSLARGHSLKHQPPSAEHESLLSGERRVVPDGERGGARGTHRHRRTLSLDTKDPSDLVEVPPALPPAHPRAPRKVGAIPGAGEHQNCNGKMPGVAKDVFTKMNDPEGHGEDEEEMDYTLCFRIRKMMDVYKPDWCEIREDWSIYLFSPQNRFRVLCQTVIAHKLFDYVVLAFIFLNCITIALERPQIENRSTERIFLTVSNYIFTAIFVAEMTLKVVSLGLYFGEQAYLRSSWNILDGFLVFVSIIDIVVSMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGLDIRNITNRSDCLAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLDKKRRKAQRLPYYATYCPTRLLIHSVCTSHYLDIFITFIICLNVVTMSLEHYNQPVSLEIALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSNLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLCPRGTSPPGPGRGAGGGAGGAVDPEGHLCMKCYSPAQENLWLDSVSLIIKDSFEGELMIIDNLSGSVFHHYSSPAVCEKCNHDTQEVQLAETEAFSLNSDKSSSVLLGDDLSLDDPGLCPPGAKGIKGEPETPDPVNVGDLGECFFPPTNLAVPPPAESYLGEMETTPFHPVPATWLEQESSPVPSSPFSPDTPSPLLPVPAEFFQPVVSASQQGQEKTARPGSLPKISLQGSWATLRPPTVNCTLLRQPPESDPSLDDSGSSSEGSLQTTLEDSLNLSDSPQRPLDLPAALFPRPTAGAHRTATAPPSPAPRRPGPRTLGLRGLRRHQRSLSSGGSTSPGCARRDSVDPSDEEGTGGGPRGGGPGGEPSEALSTLSLTALFSPPPPPPGLALTKRCGSTSSLSCSALPSRGLAAVTSRPPCSAGPYGLLSGPAWVTDFCEDPPAPSQAAECGPLPAPKLEPGPGPSKRNR

Target ORF information:

RefSeq Version	XM_003431046.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431046.2

ORF Insert Sequence:

ATGGTGGAAT ACTCCCTGGA CCTTCAGAAC ATCAACCTGT CGGCGATCCG CACAGTCCGG 
GTCCTGAGGC CCCTCAAAGC CATCAACCGG GTGCCCAGCA TGCGGATCCT GGTGAACCTA 
CTCCTGGACA CGCTGCCCAT GCTGGGAAAC GTCCTGCTGC TCTGCTTCTT CGTCTTCTTC 
ATCTTCGGCA TCATCGGCGT GCAGCTGTGG GCCGGCCTGC TCCGCAACCG CTGCTTCCTG 
GAGGACAACT TCACCCTGCA GGGCGACATC ACCCTGCCCC CTTACTACCA GCCCGAGGAG 
GACGACGAGA CGCCCTTCAT CTGCTCGCTG TCGGGGGACA ACGGCATCAT GGGGTGCCAC 
GAGATCCCGC CCCTCAAGGA GCGGGGCCAC GAGTGCTGCC TGGACAAGGA CAGCGTGGCC 
TTAGAGCGGT CCGGCCGCCA AGACCACAAC GCCAGCGGCC TGTGCGTCAA CTGGAACCAG 
TATTACAACG TGTGCCGCCC CGGCAGCGCC AACCCCCACA AGGGCGCCAT CAACTTCGAC 
AACATCGGCT ACGCCTGGAT CGTCATCTTC CAGGTGATCA CCCTGGAAGG CTGGGTGGAG 
ATCATGTACT ACGTGATGGA TGCTCATTCC TTCTACAATT TCATCTACTT TATCCTGCTC 
ATTATAGTGG GCTCCTTCTT CATGATCAAC CTGTGCCTCG TCGTCATAGC GACCCAGTTC 
TCGGAGACCA AGCAGAGGGA GCACCGGCTG ATGCAGGAGC AGAGGCAGCG GTACCTGTCG 
TCCAGCACGG TGGCCAGCTA CACGGAGCCC GGCGACTGCT ACGAGGAGAT CTTCCAGTAC 
GTCTGTCACA TCCTGCGCAA GGCCAAACGC CGCCTCCTGG GCCTCTACCA CGCCCTGCAG 
ACCCGCCGCC AGAGCCGCTG CCATCGGGGA CTGGGCCGGG GCCAGCCGGA GCCCGGGGGC 
GACCCCCCGG GGGCCTGGGG AAACGGGAAA CCACAGAAGC ACTATAAGCT TTGCCAGAAA 
CACAACCCCC TGGACTACCC GCCGCACACC CTCGTCCAGC CCATCTCGGT GACGCTGACC 
TCTGACCCCG CCAACTGCCC CAGATGCCAC CGGGCCCAGC GGGAGGCGGC CCGGCGGCTC 
TCCGGCCTGG ACAGCCCGCC CTCGGACCGG GAGGAGGGGT CCGGTGGCGG CCGGGGGGAG 
GCCAGCGGGA GCAGCGACGG CGCCTCAGAG CTGGAGAAGG AGGAGGAGGA CGGGGACCGA 
GGGGGCCTGA GGTGGCTGTG CGGGGCCGTG TGGAGGGAAA TCAGGGCCAG GCTGCGCGGG 
ATCGTCGACA GCAAGTACTT CAACCGCGGC ATCATGATCG CCATCCTGGT GAACACCATC 
AGCATGGGGA TCGAGCACCA CGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAAATCAGC 
AACGTGGTGT TCACCAGCAT GTTTGCGCTG GAGATGATCC TGAAGCTGGC AGCCTTCGGC 
CTCTTCGACT ACCTCCGCAA CCCCTACAAC ATCTTCGACA GCATCATCGT CATCATCAGC 
ATCTGGGAGA TCATCGGCCA GTCGGACGGG GGCCTGTCGG TGCTGAGGAC GTTCCGGCTG 
CTGCGCGTGC TGAAGCTGGT GCGGTTCATG CCCGCGCTGC GTCGCCAGCT GGTCGTGCTC 
ATGAAGACCA TGGACAACGT GGCCACCTTC TGCATGCTGC TCATGCTCTT CATCTTCATC 
TTCAGGACCT ATCCCAAACT CTGCACAATC CCCATGACCC CAAATGGACA CCTGGACCCC 
AACCTCCCGC TCCCCAGCCA GCCTCTGGCT GCGGGCGGGG TGCTGCCTGC CCCCCGGAAT 
TCCCTGCAGC CCGACCAGAT GCTCATCGCC CTAGGATCCC GGAAGAGCAG TGTGATGTCT 
CTGGGGAGGA TGAGCTACGA CCAGCGGTCC CTGTCCAGCT CGCGCAGCTC CTACTATGGG 
GCTTGGGGCC GCAGCGCGGC CTGGGGGAGC CGCCGTGCCA GCTGGAACAG CCTGGCCCGG 
GGCCACAGCC TGAAGCACCA GCCCCCGTCC GCCGAGCACG AGTCCCTGCT GTCGGGGGAG 
AGGCGAGTGG TGCCTGACGG GGAGCGAGGC GGGGCCCGGG GGACCCACCG CCACCGCCGG 
ACGCTCTCCC TGGACACCAA GGACCCCTCC GACCTGGTGG AGGTGCCCCC TGCCCTCCCC 
CCAGCTCACC CCCGGGCCCC CCGCAAGGTG GGTGCCATCC CGGGGGCCGG GGAACACCAG 
AACTGCAATG GCAAGATGCC CGGAGTTGCC AAGGACGTCT TCACTAAGAT GAACGACCCG 
GAGGGCCACG GGGAGGACGA GGAAGAGATG GATTACACCC TGTGTTTCCG CATCCGGAAG 
ATGATGGACG TCTACAAGCC AGACTGGTGT GAGATCCGTG AAGACTGGTC CATCTACCTC 
TTCTCCCCTC AGAACAGGTT CCGGGTGCTC TGCCAGACCG TCATCGCTCA CAAACTCTTC 
GACTACGTGG TCCTGGCCTT CATCTTCCTC AACTGCATCA CCATCGCCCT GGAGAGGCCT 
CAGATTGAGA ACCGCAGCAC GGAGAGGATC TTCCTCACAG TCTCCAACTA CATCTTCACC 
GCCATCTTCG TGGCTGAGAT GACGCTCAAG GTGGTCTCGC TGGGCCTGTA TTTCGGGGAG 
CAGGCCTATC TCCGCAGCAG CTGGAACATC CTGGACGGCT TCCTGGTCTT CGTGTCCATC 
ATCGACATCG TGGTCTCCAT GGCCTCCGCG GGCGGCGCCA AGATCCTGGG CGTCCTCCGT 
GTCCTCCGCC TCCTCCGCAC CCTCCGCCCC CTCCGGGTGA TCAGCCGGGC CCCGGGCCTG 
AAGCTGGTGG TGGAAACCCT GATCTCCTCC CTGAAGCCCA TCGGGAACAT CGTCCTAATC 
TGCTGCGCCT TCTTCATCAT CTTCGGCATC CTGGGAGTTC AGCTCTTCAA AGGGAAATTC 
TACCACTGTC TGGGCCTGGA CATCCGCAAC ATCACCAACC GCTCCGACTG CCTGGCCGCC 
AACTACCGAT GGGTGCACCA CAAGTACAAC TTCGACAACC TGGGCCAGGC CCTGATGTCC 
CTCTTCGTTC TGGCCTCGAA GGACGGCTGG GTCAATATCA TGTATAACGG ACTGGATGCG 
GTGGCTGTAG ACCAGCAGCC GGTGACCAAC AATAACCCCT GGATGCTCCT GTACTTCATC 
TCCTTCCTCC TCATCGTCAG CTTCTTCGTG CTGAACATGT TCGTGGGTGT GGTGGTGGAG 
AATTTCCACA AGTGCCGGCA GCACCAGGAG GCCGAGGAGG CCCGGCGCCG TGAGGAGAAA 
CGACTACGGC GCCTCGACAA GAAGCGCAGG AAAGCCCAGC GGCTGCCGTA CTATGCAACC 
TACTGCCCCA CTCGCCTCCT CATCCACTCT GTCTGTACCA GCCACTACCT GGACATCTTC 
ATCACCTTCA TCATCTGCCT CAACGTGGTC ACTATGTCCC TGGAGCACTA CAACCAGCCA 
GTGTCCCTGG AGATCGCCCT CAAGTACTGC AACTATATGT TCACCACCGT GTTCGTGCTG 
GAGGCCGTGC TGAAGCTGGT GGCCTTTGGG CTGAGACGCT TCTTCAAAGA CAGGTGGAAC 
CAGCTGGACC TGGCCATCGT GCTACTGTCG GTCATGGGCA TCACCCTGGA GGAGATCGAG 
ATCAACGCCG CCCTGCCCAT CAACCCCACC ATCATCCGCA TTATGAGAGT CCTGAGGATC 
GCCCGCGTAC TGAAGCTCCT GAAGATGGCC ACGGGGATGC GGGCTTTACT GGACACGGTG 
GTGCAGGCCC TTCCTCAGGT GGGGAACCTT GGTCTGCTCT TCATGCTCCT CTTCTTCATC 
TACGCAGCCC TAGGAGTGGA GCTCTTTGGG AAACTGGTGT GCAACGAGGA GAACCCCTGT 
GAGGGCATGA GTCGCCACGC CACCTTCGAG AACTTCGGCA TGGCCTTCTT GACGCTCTTC 
CAGGTCTCCA CTGGCGACAA CTGGAATGGA ATCATGAAGG ATACCCTCCG GGATTGCACC 
CACGACGAGC GCAGCTGCCT GAGCAACCTG CAGTTCATCT CGCCCCTGTA CTTCGTCAGC 
TTCGTGCTCA CGGCCCAGTT CGTCCTCATC AACGTGGTCG TGGCCGTGCT CATGAAGCAC 
CTGGACGACA GCAACAAGGA GGCTCAGGAG GATGCCGAGA TGGACGCCGA GATCGAGCTG 
GAGATGGCGC ACGGGCTGTG TCCCCGGGGG ACCAGCCCGC CGGGCCCCGG CAGGGGGGCA 
GGAGGAGGAG CCGGAGGAGC GGTCGACCCC GAGGGCCATC TCTGCATGAA GTGCTACTCT 
CCAGCCCAGG AGAACTTGTG GCTGGACAGT GTCTCTTTAA TTATTAAGGA CTCCTTCGAA 
GGGGAGTTAA TGATCATCGA TAACCTTTCA GGCTCCGTCT TCCACCATTA CTCCTCGCCG 
GCCGTGTGCG AGAAGTGTAA CCATGACACG CAAGAGGTCC AGCTGGCGGA GACGGAAGCT 
TTCTCCCTGA ACTCGGACAA GTCCTCTTCC GTCCTGCTGG GGGACGACCT CAGCCTGGAT 
GACCCCGGCC TCTGCCCTCC CGGCGCCAAG GGGATCAAGG GCGAACCAGA GACCCCGGAC 
CCCGTCAACG TGGGAGACTT GGGTGAATGT TTCTTCCCGC CGACTAACCT CGCAGTTCCT 
CCGCCTGCAG AGAGTTATCT GGGGGAGATG GAGACCACCC CCTTCCACCC CGTCCCTGCT 
ACGTGGCTTG AGCAAGAGAG CAGCCCAGTT CCTTCGAGCC CTTTCTCTCC GGACACCCCC 
AGCCCCCTCT TGCCTGTGCC AGCCGAGTTC TTTCAGCCGG TCGTCTCCGC CAGCCAGCAG 
GGCCAGGAGA AAACTGCCCG CCCCGGGAGC CTCCCCAAGA TCTCCCTGCA GGGCTCCTGG 
GCGACCCTGC GCCCCCCAAC CGTGAACTGC ACGCTTCTCC GTCAGCCCCC CGAGAGCGAC 
CCCTCCCTGG ACGACAGCGG GAGCAGCTCG GAGGGCAGCC TGCAGACCAC CCTGGAGGAC 
AGCCTGAACC TGAGCGACTC CCCACAGCGT CCCTTGGACC TCCCCGCCGC CCTCTTCCCG 
CGCCCGACGG CCGGCGCCCA CAGAACCGCG ACGGCCCCAC CGTCCCCGGC CCCCCGGCGC 
CCGGGCCCTC GCACCCTCGG CCTCCGCGGG CTCCGGCGGC ACCAACGCAG CCTCAGCAGC 
GGCGGGAGCA CCAGCCCCGG CTGCGCCCGC CGTGACTCCG TGGACCCTTC GGATGAGGAG 
GGGACGGGGG GCGGCCCCCG GGGGGGCGGC CCCGGCGGCG AACCCTCGGA GGCCCTGAGC 
ACCCTCTCTC TGACTGCCCT CTTCTCGCCC CCGCCACCGC CCCCGGGCCT GGCCCTCACC 
AAGAGATGCG GCAGCACCAG CAGCCTCTCC TGCTCCGCCC TGCCCTCCCG GGGCCTGGCG 
GCAGTGACCA GCAGACCGCC GTGCTCCGCC GGCCCCTACG GCCTCCTCTC CGGGCCCGCC 
TGGGTGACGG ACTTCTGCGA GGACCCCCCG GCCCCGAGCC AGGCGGCAGA GTGCGGCCCA 
CTGCCCGCTC CCAAGCTAGA GCCGGGACCA GGCCCGAGCA AGAGGAACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CACNA1I cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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