DENND5A cDNA ORF clone, Ornithorhynchus anatinus(platypus)

  • Gene

  • Clones

  • gRNAs

The following DENND5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOc00703 XM_007658386.1
Latest version!
Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OOc125853 XM_029060532.1
Latest version!
Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125854 XM_029060534.1
Latest version!
Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125855 XM_029060535.1
Latest version!
Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOc125856 XM_029060536.1
Latest version!
Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOc00703
    Clone ID Related Accession (Same CDS sequence) XM_007658386.1
    Accession Version XM_007658386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-04-14
    Organism Ornithorhynchus anatinus(platypus)
    Product LOW QUALITY PROTEIN: DENN domain-containing protein 5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTATAA AGGTTGCACC TGTCTTCCTT GGGCGCCCGA AAGGAATACG TACCATCAGC 
    AACAGCAGAA ATCTCGTCCG AATAAGATTA TTTATAGCTT TATGCCAGTA CATACAGGCT
    TCTAAAGCCA GAGGTGGTGC CAGCCCTTTC ATTTCCAGTG CCGCAGAAGG GGAAAATTTT
    GAGCAGACAC CTCTGAGGCG AACGTTCAAG TCCAAGGTTC TCGCCCGTTA TCCTGACAAC
    GTGGAGTGGA ATCCCTTTGA CCAGGATGCA GTGGGAATGC TGTGCATGCC CAAGGGGCTG
    GCGTTCAAGA CCCAGGCGGA CCCCAGGGAC CCCCAGTTCC ACTCTTTTAT CATCACCCGG
    GAAGACGGCT CGCGGACGTT CGGCTTCTCC CTGACGTTTT ACGAGGAGGT GACCAGCAAG
    CAGATCTGCA GCGCCATGCA GACGCTCTAC CACATGCACA ATGCCGAGTA CGACGTGTTC
    CACGTGGCCC CATCCGATGG TGGGGAGGGC AGGGGGGACT CCGAGGACTG CGACGCCACC
    CCCGGGACCA AACTGCAGCG CTTCAACTCG TACGACATCA GCCGGGACAC GCTCTACGTA
    TCCAAGTGCA TCTGCCTCAT CGCCCCCATG TCCTTCATGA AGGCCTGCAG GCGGGTATTG
    GAGCAGCTCC ACCAGGCAGT CACCTCGCCC CAGCCTCCAC CCCTCCCCCT CGAGAGCTAC
    ATCTACAACA TCCTCTACGA GGTGCCCCTC CCTCCTCCCG GGCGGCCANN TGAAATCTCG
    GGGGTCTATG GACCCATCGT CTGCCAGAGG CCGAGCACCG GCGAGTTGCC CCTCTTCGAC
    TTCCCCGTGA GGGAGGTTTT TGAGCTGCTC GGCGTGGAGA ACGTACTCCA GCTCTTTACC
    TGCGCCCTCT TGGAGTTTCA GATCCTGCTG TACTCACAGC ATTACCAGAG GCTGATGACA
    GTGGCGGAGA CGATCACGGC CCTCATGTTT CCCTTCCAGT GGCAGCACGT CTATGTGCCA
    ATCCTCCCGG CCTCGCTTCT GCACTTCTTA GACGCTCCCG TCCCCTACCT GATGGGCCTG
    CACTCCAATG GCCTGGATGA CCGGTCCAAG CTGGAACTGC CTCAGGAGGC TAACCTGTGC
    TTTGTGGACA TAGACAACCA CTTTATTGAG CTGCCTGAAG ACTTGCCTCA GTTTCCCAAC
    AAACTGGAGT TTGTCCAGGA GGTCTCCGAG ATCCTCATGG CATTTGGTAT CCCCCCGGAA
    GGGAACCTGC ACTGCAGCGA AAGTGCCTCC AAGCTGAAGA GCCTAAGGGC CTGTGACATT
    GTGTCGGACA AGCGGAACGG GAACCTTGCC AGCTCTCCTC TGAATTCCTA TGAACTTCTC
    AAGAAAAATG AGACCATCGC TCGGCTCCAA GCCCTGGTCA AAAGGACCGG GGTGAGCTTA
    GAAAAGCTTG ACGTTCGGGA GGAGACCAGT AGCAATAAGG ATCTCAAAGT CCAGTGCGAC
    GAAGAGGAGC TGAAGATTTA CCAGCTGAAC ATTCAAGTCC GGGAGGTCTT TGCAAACCGT
    TTCACTCAGA TGTTTGCCGA CTATGAGGTC TTTGTCATCC AGCCCAGTCA AGATAAGGAG
    TCCTGGTTCA CCAACAGGGA GCAGATGCAA AACTTTGATA AAGCATCTTT CTTATCAGAC
    CAGCCCGAGC CCTACCTGCC CTTCCTTTCC CGGTTCCTGG AGACGCAGAT GTTTGCCTCC
    TTTGTGGACA ATAAGATCAT GTGCCACGAT GATGATGACA AAGACCCCGT GCTGCGGGTG
    TTTGATGCGC GGGTGGACAA GATCAGGTTG CTAAATGTCA GGACCCCCAC CCTGCGGACA
    TCTATGTACC AGAAGTGCAC CACTGTGGAT GAAGCAGAGA AGGCCATTGA GATGAGGCTG
    GCCAAGATCG ACCACACGGC TGTGCACCCC CACTTACTAG ACATGAAGAT TGGGCAGGGC
    AAGTACGAGC CGGGATTCTT CCCCAAACTG CAGCCGGACG TGCTGTCCAC CGGCCCGGCC
    AGCAATAAGT GGACGAAGAG GAACGCACCC GCCCAGTGGA GGCGGAAGGA TCGGCAGAAG
    CAACACACGG AGCATCTACG CCTGGACAAC GATCAGAGGG AGCGGTTGGA CTTTTCCGCT
    CCCAAGCTTC AGATGCAGTT TTATTTAGCA CGGAGCCAGC CCGGGTGGTC TTTCAGGTCC
    TCTCTAAAAC CGATGTCCGA CCTTTACCTG AAATACATCC AGGAGGCCAG GAACCTTGGC
    AGCAACATCC GCCAGCCCAA ATTATCGAAC CTCTCACCAT CCGTGATTGC GCAGACCAAC
    TGGAAGTTTG TTGAGGGTTT ACTGAAAGAA TGCCGCAACA AGACCAAGAG GATGCTGGTG
    GAGAAGATGG GCCGGGAAGC CGTGGAGCTG GGCCACGGGG AGGTGAACAT CACTGGCGTA
    GAAGAGAACA CTCTGATCGC CAGCCTCTGT GATCTGCTGG AGAGAATTTG GAGCCACGGC
    CTGCAGATCA AGCAGGGTAA ATCAGCCTTG TGGTCTCACT TGTTACATTA CCAGGAAAAC
    AGGCAGAGGA GGCCAACGTC AGGAAGTTTA AGTACCTCAG GAATCCTTCT CGATTCAGAA
    CGGAGGAAGT CTGATGCCAG TCCAGCGATG TCTCCTCTAA GGGTGTCTCT CATTCAGGAT
    ATGAGGCACA TCCAGAACAT TGGAGAAATC AAAACAGATG TCGGGAAGGC CCGAGCCTGG
    GTCCGGCTGT CCATGGAAAA GAAGTTGCTT TCCAGACACC TCAAACAGCT TCTTTCGGAT
    CATGAACTGA CCAAGAAGCT GTACAAGCGT TACGCCTTTC TGCGCTGTGA CGATGAGAAG
    GAGCAGTTCC TCTACCACCT GCTCTCCTTC AATGCTGTCG ATTACTTCTG CTTCACCAAC
    GTCTTCACCA CCATCATGAT CCCGTACCAC ATCCTGATCG TCCCAAGCAA GAAGCTCGGT
    GGGTCCATGT TCACTGCCAA TCCCTGGCTC TGCATTTCGG GCGAGCTGGG CGAGACACAG
    ATCCTGCAGA TCCCCAGGAA CGTCTTGGAG ATGACCTTCG AGTGGCAGAA CCTGGGGAAG
    CTTACCACCG TCCAGATCGG GCACGACAAC TCAGGCCTCT ATGCCAAGTG GCTCGTGGAG
    TATGTGATGG TCAGAAACGA AATCACGGGA CACACGTACA AATTCCCCTG TGGCCGGTGG
    CTGGGGAAGG GTACGGACGA TGGGAGTCTG GAGAGGGTCC TGGTCGGGGA GCTGCTCACA
    TCGCACCCTG AGGCAGATGA GCGGCAGTCT CGGACCCCAC CCCTGCAGCA GTCCCCCAGC
    ATGATTAGGA GACTGGTCAC CATCTCCCCC AACAACAAGC CAAAGTTGAA CACGGGGCAG
    ATTCAGGAGT CCATTGGGGA AGCGGTCAAT GGCATCGTGA AGCATTTCCA CAAGCCTGAG
    AAAGAGAGAG GCAGCCTGAC CCTGCTGCTG TGTGGGGAGT GCGGGCTCGT GTCTGCCCTG
    GAACAAGTTT TTCAGCACGG TTTTAAATCG CCCCGGCTCT TCAAGAACGT CTTCATCTGG
    GACTTCCTGG AAAAAGCACA AACCTACTAT GAGACCCTGG ACCAGAGTGA GATGGTCCCT
    GAGGAGAACT GGCAGACACG GGCCCGGAAC TTCTGCCGCT TCATCACAGC AATCAACAAC
    ACCCCACGGA ACATCGGCAA GGACGGAAAA TTCCAGACGC TGGTGTGCCT GGGTGCCAGA
    GACCACCTCC TCCACCACTG GATCGCTTTG CTGGCGGACT GCCCCATCAC GGCACAGATG
    TATGAGGACG TGGCATTGAT TAAGGACCAC ACCCTTGTCA ATTCCTTGAT CAGAGTGCTG
    CAGACCTTAC AGGAATTCAA CATCACTCTG GAGACGTCGC TCGTCAAAGG GATTGATATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007656576.1
    CDS1..3963
    Translation

    Target ORF information:

    RefSeq Version XM_007658386.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_007658386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTATAA AGGTTGCACC TGTCTTCCTT GGGCGCCCGA AAGGAATACG TACCATCAGC 
    AACAGCAGAA ATCTCGTCCG AATAAGATTA TTTATAGCTT TATGCCAGTA CATACAGGCT
    TCTAAAGCCA GAGGTGGTGC CAGCCCTTTC ATTTCCAGTG CCGCAGAAGG GGAAAATTTT
    GAGCAGACAC CTCTGAGGCG AACGTTCAAG TCCAAGGTTC TCGCCCGTTA TCCTGACAAC
    GTGGAGTGGA ATCCCTTTGA CCAGGATGCA GTGGGAATGC TGTGCATGCC CAAGGGGCTG
    GCGTTCAAGA CCCAGGCGGA CCCCAGGGAC CCCCAGTTCC ACTCTTTTAT CATCACCCGG
    GAAGACGGCT CGCGGACGTT CGGCTTCTCC CTGACGTTTT ACGAGGAGGT GACCAGCAAG
    CAGATCTGCA GCGCCATGCA GACGCTCTAC CACATGCACA ATGCCGAGTA CGACGTGTTC
    CACGTGGCCC CATCCGATGG TGGGGAGGGC AGGGGGGACT CCGAGGACTG CGACGCCACC
    CCCGGGACCA AACTGCAGCG CTTCAACTCG TACGACATCA GCCGGGACAC GCTCTACGTA
    TCCAAGTGCA TCTGCCTCAT CGCCCCCATG TCCTTCATGA AGGCCTGCAG GCGGGTATTG
    GAGCAGCTCC ACCAGGCAGT CACCTCGCCC CAGCCTCCAC CCCTCCCCCT CGAGAGCTAC
    ATCTACAACA TCCTCTACGA GGTGCCCCTC CCTCCTCCCG GGCGGCCANN TGAAATCTCG
    GGGGTCTATG GACCCATCGT CTGCCAGAGG CCGAGCACCG GCGAGTTGCC CCTCTTCGAC
    TTCCCCGTGA GGGAGGTTTT TGAGCTGCTC GGCGTGGAGA ACGTACTCCA GCTCTTTACC
    TGCGCCCTCT TGGAGTTTCA GATCCTGCTG TACTCACAGC ATTACCAGAG GCTGATGACA
    GTGGCGGAGA CGATCACGGC CCTCATGTTT CCCTTCCAGT GGCAGCACGT CTATGTGCCA
    ATCCTCCCGG CCTCGCTTCT GCACTTCTTA GACGCTCCCG TCCCCTACCT GATGGGCCTG
    CACTCCAATG GCCTGGATGA CCGGTCCAAG CTGGAACTGC CTCAGGAGGC TAACCTGTGC
    TTTGTGGACA TAGACAACCA CTTTATTGAG CTGCCTGAAG ACTTGCCTCA GTTTCCCAAC
    AAACTGGAGT TTGTCCAGGA GGTCTCCGAG ATCCTCATGG CATTTGGTAT CCCCCCGGAA
    GGGAACCTGC ACTGCAGCGA AAGTGCCTCC AAGCTGAAGA GCCTAAGGGC CTGTGACATT
    GTGTCGGACA AGCGGAACGG GAACCTTGCC AGCTCTCCTC TGAATTCCTA TGAACTTCTC
    AAGAAAAATG AGACCATCGC TCGGCTCCAA GCCCTGGTCA AAAGGACCGG GGTGAGCTTA
    GAAAAGCTTG ACGTTCGGGA GGAGACCAGT AGCAATAAGG ATCTCAAAGT CCAGTGCGAC
    GAAGAGGAGC TGAAGATTTA CCAGCTGAAC ATTCAAGTCC GGGAGGTCTT TGCAAACCGT
    TTCACTCAGA TGTTTGCCGA CTATGAGGTC TTTGTCATCC AGCCCAGTCA AGATAAGGAG
    TCCTGGTTCA CCAACAGGGA GCAGATGCAA AACTTTGATA AAGCATCTTT CTTATCAGAC
    CAGCCCGAGC CCTACCTGCC CTTCCTTTCC CGGTTCCTGG AGACGCAGAT GTTTGCCTCC
    TTTGTGGACA ATAAGATCAT GTGCCACGAT GATGATGACA AAGACCCCGT GCTGCGGGTG
    TTTGATGCGC GGGTGGACAA GATCAGGTTG CTAAATGTCA GGACCCCCAC CCTGCGGACA
    TCTATGTACC AGAAGTGCAC CACTGTGGAT GAAGCAGAGA AGGCCATTGA GATGAGGCTG
    GCCAAGATCG ACCACACGGC TGTGCACCCC CACTTACTAG ACATGAAGAT TGGGCAGGGC
    AAGTACGAGC CGGGATTCTT CCCCAAACTG CAGCCGGACG TGCTGTCCAC CGGCCCGGCC
    AGCAATAAGT GGACGAAGAG GAACGCACCC GCCCAGTGGA GGCGGAAGGA TCGGCAGAAG
    CAACACACGG AGCATCTACG CCTGGACAAC GATCAGAGGG AGCGGTTGGA CTTTTCCGCT
    CCCAAGCTTC AGATGCAGTT TTATTTAGCA CGGAGCCAGC CCGGGTGGTC TTTCAGGTCC
    TCTCTAAAAC CGATGTCCGA CCTTTACCTG AAATACATCC AGGAGGCCAG GAACCTTGGC
    AGCAACATCC GCCAGCCCAA ATTATCGAAC CTCTCACCAT CCGTGATTGC GCAGACCAAC
    TGGAAGTTTG TTGAGGGTTT ACTGAAAGAA TGCCGCAACA AGACCAAGAG GATGCTGGTG
    GAGAAGATGG GCCGGGAAGC CGTGGAGCTG GGCCACGGGG AGGTGAACAT CACTGGCGTA
    GAAGAGAACA CTCTGATCGC CAGCCTCTGT GATCTGCTGG AGAGAATTTG GAGCCACGGC
    CTGCAGATCA AGCAGGGTAA ATCAGCCTTG TGGTCTCACT TGTTACATTA CCAGGAAAAC
    AGGCAGAGGA GGCCAACGTC AGGAAGTTTA AGTACCTCAG GAATCCTTCT CGATTCAGAA
    CGGAGGAAGT CTGATGCCAG TCCAGCGATG TCTCCTCTAA GGGTGTCTCT CATTCAGGAT
    ATGAGGCACA TCCAGAACAT TGGAGAAATC AAAACAGATG TCGGGAAGGC CCGAGCCTGG
    GTCCGGCTGT CCATGGAAAA GAAGTTGCTT TCCAGACACC TCAAACAGCT TCTTTCGGAT
    CATGAACTGA CCAAGAAGCT GTACAAGCGT TACGCCTTTC TGCGCTGTGA CGATGAGAAG
    GAGCAGTTCC TCTACCACCT GCTCTCCTTC AATGCTGTCG ATTACTTCTG CTTCACCAAC
    GTCTTCACCA CCATCATGAT CCCGTACCAC ATCCTGATCG TCCCAAGCAA GAAGCTCGGT
    GGGTCCATGT TCACTGCCAA TCCCTGGCTC TGCATTTCGG GCGAGCTGGG CGAGACACAG
    ATCCTGCAGA TCCCCAGGAA CGTCTTGGAG ATGACCTTCG AGTGGCAGAA CCTGGGGAAG
    CTTACCACCG TCCAGATCGG GCACGACAAC TCAGGCCTCT ATGCCAAGTG GCTCGTGGAG
    TATGTGATGG TCAGAAACGA AATCACGGGA CACACGTACA AATTCCCCTG TGGCCGGTGG
    CTGGGGAAGG GTACGGACGA TGGGAGTCTG GAGAGGGTCC TGGTCGGGGA GCTGCTCACA
    TCGCACCCTG AGGCAGATGA GCGGCAGTCT CGGACCCCAC CCCTGCAGCA GTCCCCCAGC
    ATGATTAGGA GACTGGTCAC CATCTCCCCC AACAACAAGC CAAAGTTGAA CACGGGGCAG
    ATTCAGGAGT CCATTGGGGA AGCGGTCAAT GGCATCGTGA AGCATTTCCA CAAGCCTGAG
    AAAGAGAGAG GCAGCCTGAC CCTGCTGCTG TGTGGGGAGT GCGGGCTCGT GTCTGCCCTG
    GAACAAGTTT TTCAGCACGG TTTTAAATCG CCCCGGCTCT TCAAGAACGT CTTCATCTGG
    GACTTCCTGG AAAAAGCACA AACCTACTAT GAGACCCTGG ACCAGAGTGA GATGGTCCCT
    GAGGAGAACT GGCAGACACG GGCCCGGAAC TTCTGCCGCT TCATCACAGC AATCAACAAC
    ACCCCACGGA ACATCGGCAA GGACGGAAAA TTCCAGACGC TGGTGTGCCT GGGTGCCAGA
    GACCACCTCC TCCACCACTG GATCGCTTTG CTGGCGGACT GCCCCATCAC GGCACAGATG
    TATGAGGACG TGGCATTGAT TAAGGACCAC ACCCTTGTCA ATTCCTTGAT CAGAGTGCTG
    CAGACCTTAC AGGAATTCAA CATCACTCTG GAGACGTCGC TCGTCAAAGG GATTGATATC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125853
    Clone ID Related Accession (Same CDS sequence) XM_029060532.1
    Accession Version XM_029060532.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product DENN domain-containing protein 5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GCTTTATGCC AGTACATACA GGCTTCTAAA GCCAGAGGTG GTGCCAGCCC TTTCATTTCC
    AGTGCCGCAG AAGGGGAAAA TTTTGAGCAG ACACCTCTGA GGCGAACGTT CAAGTCCAAG
    GTTCTCGCCC GTTATCCTGA CAACGTGGAG TGGAATCCCT TTGACCAGGA TGCAGTGGGA
    ATGCTGTGCA TGCCCAAGGG GCTGGCATTC AAGACCCAGG CGGACCCCAG GGACCCCCAG
    TTCCACTCTT TTATCATCAC CCGGGAAGAC GGCTCGCGGA CGTTCGGCTT CTCCCTGACG
    TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGCGCCA TGCAGACGCT CTACCACATG
    CACAATGCCG AGTACGACGT GTTCCACGTG GCCCCATCCG ATGGTGGGGA GGGCAGGGGG
    GACTCCGAGG ACTGCGACGC CACCCCCGGG ACCAAACTGC AGCGCTTCAA CTCGTACGAC
    ATCAGCCGGG ACACGCTCTA CGTATCCAAG TGCATCTGCC TCATCGCCCC CATGTCCTTC
    ATGAAGGCCT GCAGGCGGGT ATTGGAGCAG CTCCACCAGG CAGTCACCTC GCCCCAGCCT
    CCACCCCTCC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTCCCTCCT
    CCCGGGCGGT CCCTGAAATT CTCGGGGGTC TATGGACCCA TCGTCTGCCA GAGGCCGAGC
    ACCGGCGAGT TGCCCCTCTT CGACTTCCCC GTGAGGGAGG TTTTTGAGCT GCTCGGCGTG
    GAGAACGTAC TCCAGCTCTT TACCTGCGCC CTCTTGGAGT TTCAGATCCT GCTGTACTCA
    CAGCATTACC AGAGGCTGAT GACAGTGGCG GAGACGATCA CGGCCCTCAT GTTTCCCTTC
    CAGTGGCAGC ACGTCTATGT GCCAATCCTC CCGGCCTCGC TTCTGCACTT CTTAGACGCT
    CCCGTCCCCT ACCTGATGGG CCTGCACTCC AATGGCCTGG ATGACCGGTC CAAGCTGGAA
    CTGCCTCAGG AGGCTAACCT GTGCTTTGTG GACATAGACA ACCACTTTAT TGAGCTGCCT
    GAAGACTTGC CTCAGTTTCC CAACAAACTG GAGTTTGTCC AGGAGGTCTC CGAGATCCTC
    ATGGCATTTG GTATCCCCCC GGAAGGGAAC CTGCACTGCA GCGAAAGTGC CTCCAAGCTG
    AAGAGCCTAA GGGCCTGTGA CATTGTGTCG GACAAGCGGA ACGGGAACCT TGCCAGCTCT
    CCTCTGAATT CCTATGAACT TCTCAAGAAA AATGAGACCA TCGCTCGGCT CCAAGCCCTG
    GTCAAAAGGA CCGGGGTGAG CTTAGAAAAG CTTGACGTTC GGGAGGAGAC CAGTAGCAAT
    AAGGATCTCA AAGTCCAGTG CGACGAAGAG GAGCTGAAGA TTTACCAGCT GAACATTCAA
    GTCCGGGAGG TCTTTGCAAA CCGTTTCACT CAGATGTTTG CCGACTATGA GGTCTTTGTC
    ATCCAGCCCA GTCAAGATAA GGAGTCCTGG TTCACCAACA GGGAGCAGAT GCAAAACTTT
    GATAAAGCAT CTTTCTTATC AGACCAGCCC GAGCCCTACC TGCCCTTCCT TTCCCGGTTC
    CTGGAGACGC AGATGTTTGC CTCCTTTGTG GACAATAAGA TCATGTGCCA CGATGATGAT
    GACAAAGACC CCGTGCTGCG GGTGTTTGAT GCGCGGGTGG ACAAGATCAG GTTGCTAAAT
    GTCAGGACCC CCACCCTGCG GACATCTATG TACCAGAAGT GCACCACTGT GGATGAAGCA
    GAGAAGGCCA TTGAGATGAG GCTGGCCAAG ATCGACCACA CGGCTGTGCA CCCCCACTTA
    CTAGACATGA AGATTGGGCA GGGCAAGTAC GAGCCGGGAT TCTTCCCCAA ACTGCAGCCG
    GACGTGCTGT CCACCGGCCC GGCCAGCAAT AAGTGGACGA AGAGGAACGC ACCCGCCCAG
    TGGAGGCGGA AGGATCGGCA GAAGCAACAC ACGGAGCATC TACGCCTGGA CAACGATCAG
    AGGGAGCGGT TGGACTTTTC CGCTCCCAAG CTTCAGATGC AGTTTTATTT AGCACGGAGC
    CAGCCCGGGT GGTCTTTCAG GTCCTCTCTA AAACCGATGT CCGACCTTTA CCTGAAATAC
    ATCCAGGAGG CCAGGAACCT TGGCAGCAAC ATCCGCCAGC CCAAATTATC GAACCTCTCA
    CCATCCGTGA TTGCGCAGAC CAACTGGAAG TTTGTTGAGG GTTTACTGAA AGAATGCCGC
    AACAAGACCA AGAGGATGCT GGTGGAGAAG ATGGGCCGGG AAGCCGTGGA GCTGGGCCAC
    GGGGAGGTGA ACATCACTGG CGTAGAAGAG AACACTCTGA TCGCCAGCCT CTGTGATCTG
    CTGGAGAGAA TTTGGAGCCA CGGCCTGCAG ATCAAGCAGG GTAAATCAGC CTTGTGGTCT
    CACTTGTTAC ATTACCAGGA AAACAGGCAG AGGAGGCCAA CGTCAGGAAG TTTAAGTACC
    TCAGGAATCC TTCTCGATTC AGAACGGAGG AAGTCTGATG CCAGTCCAGC GATGTCTCCT
    CTAAGGGTGT CTCTCATTCA GGATATGAGG CACATCCAGA ACATTGGAGA AATCAAAACA
    GATGTCGGGA AGGCCCGAGC CTGGGTCCGG CTGTCCATGG AAAAGAAGTT GCTTTCCAGA
    CACCTCAAAC AGCTTCTTTC GGATCATGAA CTGACCAAGA AGCTGTACAA GCGTTACGCC
    TTTCTGCGCT GTGACGATGA GAAGGAGCAG TTCCTCTACC ACCTGCTCTC CTTCAATGCT
    GTCGATTACT TCTGCTTCAC CAACGTCTTC ACCACCATCA TGATCCCGTA CCACATCCTG
    ATCGTCCCAA GCAAGAAGCT CGGTGGGTCC ATGTTCACTG CCAATCCCTG GCTCTGCATT
    TCGGGCGAGC TGGGCGAGAC ACAGATCCTG CAGATCCCCA GGAACGTCTT GGAGATGACC
    TTCGAGTGGC AGAACCTGGG GAAGCTTACC ACCGTCCAGA TCGGGCACGA CAACTCAGGC
    CTCTATGCCA AGTGGCTCGT GGAGTATGTG ATGGTCAGAA ACGAAATCAC GGGACACACG
    TACAAATTCC CCTGTGGCCG GTGGCTGGGG AAGGGTACGG ACGATGGGAG TCTGGAGAGG
    GTCCTGGTCG GGGAGCTGCT CACATCGCAC CCTGAGGCAG ATGAGCGGCA GTCTCGGACC
    CCACCCCTGC AGCAGTCCCC CAGCATGATT AGGAGACTGG TCACCATCTC CCCCAACAAC
    AAGCCAAAGT TGAACACGGG GCAGATTCAG GAGTCCATTG GGGAAGCGGT CAATGGCATC
    GTGAAGCATT TCCACAAGCC TGAGAAAGAG AGAGGCAGCC TGACCCTGCT GCTGTGTGGG
    GAGTGCGGGC TCGTGTCTGC CCTGGAACAA GTTTTTCAGC ACGGTTTTAA ATCGCCCCGG
    CTCTTCAAGA ACGTCTTCAT CTGGGACTTC CTGGAAAAAG CACAAACCTA CTATGAGACC
    CTGGACCAGA GTGAGATGGT CCCTGAGGAG AACTGGCAGA CACGGGCCCG GAACTTCTGC
    CGCTTCATCA CAGCAATCAA CAACACCCCA CGGAACATCG GCAAGGACGG AAAATTCCAG
    ACGCTGGTGT GCCTGGGTGC CAGAGACCAC CTCCTCCACC ACTGGATCGC TTTGCTGGCG
    GACTGCCCCA TCACGGCACA GATGTATGAG GACGTGGCAT TGATTAAGGA CCACACCCTT
    GTCAATTCCT TGATCAGAGT GCTGCAGACC TTACAGGAAT TCAACATCAC TCTGGAGACG
    TCGCTCGTCA AAGGGATTGA TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028916365.1
    CDS350..4336
    Translation

    Target ORF information:

    RefSeq Version XM_029060532.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029060532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GCTTTATGCC AGTACATACA GGCTTCTAAA GCCAGAGGTG GTGCCAGCCC TTTCATTTCC
    AGTGCCGCAG AAGGGGAAAA TTTTGAGCAG ACACCTCTGA GGCGAACGTT CAAGTCCAAG
    GTTCTCGCCC GTTATCCTGA CAACGTGGAG TGGAATCCCT TTGACCAGGA TGCAGTGGGA
    ATGCTGTGCA TGCCCAAGGG GCTGGCATTC AAGACCCAGG CGGACCCCAG GGACCCCCAG
    TTCCACTCTT TTATCATCAC CCGGGAAGAC GGCTCGCGGA CGTTCGGCTT CTCCCTGACG
    TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGCGCCA TGCAGACGCT CTACCACATG
    CACAATGCCG AGTACGACGT GTTCCACGTG GCCCCATCCG ATGGTGGGGA GGGCAGGGGG
    GACTCCGAGG ACTGCGACGC CACCCCCGGG ACCAAACTGC AGCGCTTCAA CTCGTACGAC
    ATCAGCCGGG ACACGCTCTA CGTATCCAAG TGCATCTGCC TCATCGCCCC CATGTCCTTC
    ATGAAGGCCT GCAGGCGGGT ATTGGAGCAG CTCCACCAGG CAGTCACCTC GCCCCAGCCT
    CCACCCCTCC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTCCCTCCT
    CCCGGGCGGT CCCTGAAATT CTCGGGGGTC TATGGACCCA TCGTCTGCCA GAGGCCGAGC
    ACCGGCGAGT TGCCCCTCTT CGACTTCCCC GTGAGGGAGG TTTTTGAGCT GCTCGGCGTG
    GAGAACGTAC TCCAGCTCTT TACCTGCGCC CTCTTGGAGT TTCAGATCCT GCTGTACTCA
    CAGCATTACC AGAGGCTGAT GACAGTGGCG GAGACGATCA CGGCCCTCAT GTTTCCCTTC
    CAGTGGCAGC ACGTCTATGT GCCAATCCTC CCGGCCTCGC TTCTGCACTT CTTAGACGCT
    CCCGTCCCCT ACCTGATGGG CCTGCACTCC AATGGCCTGG ATGACCGGTC CAAGCTGGAA
    CTGCCTCAGG AGGCTAACCT GTGCTTTGTG GACATAGACA ACCACTTTAT TGAGCTGCCT
    GAAGACTTGC CTCAGTTTCC CAACAAACTG GAGTTTGTCC AGGAGGTCTC CGAGATCCTC
    ATGGCATTTG GTATCCCCCC GGAAGGGAAC CTGCACTGCA GCGAAAGTGC CTCCAAGCTG
    AAGAGCCTAA GGGCCTGTGA CATTGTGTCG GACAAGCGGA ACGGGAACCT TGCCAGCTCT
    CCTCTGAATT CCTATGAACT TCTCAAGAAA AATGAGACCA TCGCTCGGCT CCAAGCCCTG
    GTCAAAAGGA CCGGGGTGAG CTTAGAAAAG CTTGACGTTC GGGAGGAGAC CAGTAGCAAT
    AAGGATCTCA AAGTCCAGTG CGACGAAGAG GAGCTGAAGA TTTACCAGCT GAACATTCAA
    GTCCGGGAGG TCTTTGCAAA CCGTTTCACT CAGATGTTTG CCGACTATGA GGTCTTTGTC
    ATCCAGCCCA GTCAAGATAA GGAGTCCTGG TTCACCAACA GGGAGCAGAT GCAAAACTTT
    GATAAAGCAT CTTTCTTATC AGACCAGCCC GAGCCCTACC TGCCCTTCCT TTCCCGGTTC
    CTGGAGACGC AGATGTTTGC CTCCTTTGTG GACAATAAGA TCATGTGCCA CGATGATGAT
    GACAAAGACC CCGTGCTGCG GGTGTTTGAT GCGCGGGTGG ACAAGATCAG GTTGCTAAAT
    GTCAGGACCC CCACCCTGCG GACATCTATG TACCAGAAGT GCACCACTGT GGATGAAGCA
    GAGAAGGCCA TTGAGATGAG GCTGGCCAAG ATCGACCACA CGGCTGTGCA CCCCCACTTA
    CTAGACATGA AGATTGGGCA GGGCAAGTAC GAGCCGGGAT TCTTCCCCAA ACTGCAGCCG
    GACGTGCTGT CCACCGGCCC GGCCAGCAAT AAGTGGACGA AGAGGAACGC ACCCGCCCAG
    TGGAGGCGGA AGGATCGGCA GAAGCAACAC ACGGAGCATC TACGCCTGGA CAACGATCAG
    AGGGAGCGGT TGGACTTTTC CGCTCCCAAG CTTCAGATGC AGTTTTATTT AGCACGGAGC
    CAGCCCGGGT GGTCTTTCAG GTCCTCTCTA AAACCGATGT CCGACCTTTA CCTGAAATAC
    ATCCAGGAGG CCAGGAACCT TGGCAGCAAC ATCCGCCAGC CCAAATTATC GAACCTCTCA
    CCATCCGTGA TTGCGCAGAC CAACTGGAAG TTTGTTGAGG GTTTACTGAA AGAATGCCGC
    AACAAGACCA AGAGGATGCT GGTGGAGAAG ATGGGCCGGG AAGCCGTGGA GCTGGGCCAC
    GGGGAGGTGA ACATCACTGG CGTAGAAGAG AACACTCTGA TCGCCAGCCT CTGTGATCTG
    CTGGAGAGAA TTTGGAGCCA CGGCCTGCAG ATCAAGCAGG GTAAATCAGC CTTGTGGTCT
    CACTTGTTAC ATTACCAGGA AAACAGGCAG AGGAGGCCAA CGTCAGGAAG TTTAAGTACC
    TCAGGAATCC TTCTCGATTC AGAACGGAGG AAGTCTGATG CCAGTCCAGC GATGTCTCCT
    CTAAGGGTGT CTCTCATTCA GGATATGAGG CACATCCAGA ACATTGGAGA AATCAAAACA
    GATGTCGGGA AGGCCCGAGC CTGGGTCCGG CTGTCCATGG AAAAGAAGTT GCTTTCCAGA
    CACCTCAAAC AGCTTCTTTC GGATCATGAA CTGACCAAGA AGCTGTACAA GCGTTACGCC
    TTTCTGCGCT GTGACGATGA GAAGGAGCAG TTCCTCTACC ACCTGCTCTC CTTCAATGCT
    GTCGATTACT TCTGCTTCAC CAACGTCTTC ACCACCATCA TGATCCCGTA CCACATCCTG
    ATCGTCCCAA GCAAGAAGCT CGGTGGGTCC ATGTTCACTG CCAATCCCTG GCTCTGCATT
    TCGGGCGAGC TGGGCGAGAC ACAGATCCTG CAGATCCCCA GGAACGTCTT GGAGATGACC
    TTCGAGTGGC AGAACCTGGG GAAGCTTACC ACCGTCCAGA TCGGGCACGA CAACTCAGGC
    CTCTATGCCA AGTGGCTCGT GGAGTATGTG ATGGTCAGAA ACGAAATCAC GGGACACACG
    TACAAATTCC CCTGTGGCCG GTGGCTGGGG AAGGGTACGG ACGATGGGAG TCTGGAGAGG
    GTCCTGGTCG GGGAGCTGCT CACATCGCAC CCTGAGGCAG ATGAGCGGCA GTCTCGGACC
    CCACCCCTGC AGCAGTCCCC CAGCATGATT AGGAGACTGG TCACCATCTC CCCCAACAAC
    AAGCCAAAGT TGAACACGGG GCAGATTCAG GAGTCCATTG GGGAAGCGGT CAATGGCATC
    GTGAAGCATT TCCACAAGCC TGAGAAAGAG AGAGGCAGCC TGACCCTGCT GCTGTGTGGG
    GAGTGCGGGC TCGTGTCTGC CCTGGAACAA GTTTTTCAGC ACGGTTTTAA ATCGCCCCGG
    CTCTTCAAGA ACGTCTTCAT CTGGGACTTC CTGGAAAAAG CACAAACCTA CTATGAGACC
    CTGGACCAGA GTGAGATGGT CCCTGAGGAG AACTGGCAGA CACGGGCCCG GAACTTCTGC
    CGCTTCATCA CAGCAATCAA CAACACCCCA CGGAACATCG GCAAGGACGG AAAATTCCAG
    ACGCTGGTGT GCCTGGGTGC CAGAGACCAC CTCCTCCACC ACTGGATCGC TTTGCTGGCG
    GACTGCCCCA TCACGGCACA GATGTATGAG GACGTGGCAT TGATTAAGGA CCACACCCTT
    GTCAATTCCT TGATCAGAGT GCTGCAGACC TTACAGGAAT TCAACATCAC TCTGGAGACG
    TCGCTCGTCA AAGGGATTGA TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125854
    Clone ID Related Accession (Same CDS sequence) XM_029060534.1
    Accession Version XM_029060534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product DENN domain-containing protein 5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GGGGAAAATT TTGAGCAGAC ACCTCTGAGG CGAACGTTCA AGTCCAAGGT TCTCGCCCGT
    TATCCTGACA ACGTGGAGTG GAATCCCTTT GACCAGGATG CAGTGGGAAT GCTGTGCATG
    CCCAAGGGGC TGGCATTCAA GACCCAGGCG GACCCCAGGG ACCCCCAGTT CCACTCTTTT
    ATCATCACCC GGGAAGACGG CTCGCGGACG TTCGGCTTCT CCCTGACGTT TTACGAGGAG
    GTGACCAGCA AGCAGATCTG CAGCGCCATG CAGACGCTCT ACCACATGCA CAATGCCGAG
    TACGACGTGT TCCACGTGGC CCCATCCGAT GGTGGGGAGG GCAGGGGGGA CTCCGAGGAC
    TGCGACGCCA CCCCCGGGAC CAAACTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC
    ACGCTCTACG TATCCAAGTG CATCTGCCTC ATCGCCCCCA TGTCCTTCAT GAAGGCCTGC
    AGGCGGGTAT TGGAGCAGCT CCACCAGGCA GTCACCTCGC CCCAGCCTCC ACCCCTCCCC
    CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TCCCTCCTCC CGGGCGGTCC
    CTGAAATTCT CGGGGGTCTA TGGACCCATC GTCTGCCAGA GGCCGAGCAC CGGCGAGTTG
    CCCCTCTTCG ACTTCCCCGT GAGGGAGGTT TTTGAGCTGC TCGGCGTGGA GAACGTACTC
    CAGCTCTTTA CCTGCGCCCT CTTGGAGTTT CAGATCCTGC TGTACTCACA GCATTACCAG
    AGGCTGATGA CAGTGGCGGA GACGATCACG GCCCTCATGT TTCCCTTCCA GTGGCAGCAC
    GTCTATGTGC CAATCCTCCC GGCCTCGCTT CTGCACTTCT TAGACGCTCC CGTCCCCTAC
    CTGATGGGCC TGCACTCCAA TGGCCTGGAT GACCGGTCCA AGCTGGAACT GCCTCAGGAG
    GCTAACCTGT GCTTTGTGGA CATAGACAAC CACTTTATTG AGCTGCCTGA AGACTTGCCT
    CAGTTTCCCA ACAAACTGGA GTTTGTCCAG GAGGTCTCCG AGATCCTCAT GGCATTTGGT
    ATCCCCCCGG AAGGGAACCT GCACTGCAGC GAAAGTGCCT CCAAGCTGAA GAGCCTAAGG
    GCCTGTGACA TTGTGTCGGA CAAGCGGAAC GGGAACCTTG CCAGCTCTCC TCTGAATTCC
    TATGAACTTC TCAAGAAAAA TGAGACCATC GCTCGGCTCC AAGCCCTGGT CAAAAGGACC
    GGGGTGAGCT TAGAAAAGCT TGACGTTCGG GAGGAGACCA GTAGCAATAA GGATCTCAAA
    GTCCAGTGCG ACGAAGAGGA GCTGAAGATT TACCAGCTGA ACATTCAAGT CCGGGAGGTC
    TTTGCAAACC GTTTCACTCA GATGTTTGCC GACTATGAGG TCTTTGTCAT CCAGCCCAGT
    CAAGATAAGG AGTCCTGGTT CACCAACAGG GAGCAGATGC AAAACTTTGA TAAAGCATCT
    TTCTTATCAG ACCAGCCCGA GCCCTACCTG CCCTTCCTTT CCCGGTTCCT GGAGACGCAG
    ATGTTTGCCT CCTTTGTGGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC
    GTGCTGCGGG TGTTTGATGC GCGGGTGGAC AAGATCAGGT TGCTAAATGT CAGGACCCCC
    ACCCTGCGGA CATCTATGTA CCAGAAGTGC ACCACTGTGG ATGAAGCAGA GAAGGCCATT
    GAGATGAGGC TGGCCAAGAT CGACCACACG GCTGTGCACC CCCACTTACT AGACATGAAG
    ATTGGGCAGG GCAAGTACGA GCCGGGATTC TTCCCCAAAC TGCAGCCGGA CGTGCTGTCC
    ACCGGCCCGG CCAGCAATAA GTGGACGAAG AGGAACGCAC CCGCCCAGTG GAGGCGGAAG
    GATCGGCAGA AGCAACACAC GGAGCATCTA CGCCTGGACA ACGATCAGAG GGAGCGGTTG
    GACTTTTCCG CTCCCAAGCT TCAGATGCAG TTTTATTTAG CACGGAGCCA GCCCGGGTGG
    TCTTTCAGGT CCTCTCTAAA ACCGATGTCC GACCTTTACC TGAAATACAT CCAGGAGGCC
    AGGAACCTTG GCAGCAACAT CCGCCAGCCC AAATTATCGA ACCTCTCACC ATCCGTGATT
    GCGCAGACCA ACTGGAAGTT TGTTGAGGGT TTACTGAAAG AATGCCGCAA CAAGACCAAG
    AGGATGCTGG TGGAGAAGAT GGGCCGGGAA GCCGTGGAGC TGGGCCACGG GGAGGTGAAC
    ATCACTGGCG TAGAAGAGAA CACTCTGATC GCCAGCCTCT GTGATCTGCT GGAGAGAATT
    TGGAGCCACG GCCTGCAGAT CAAGCAGGGT AAATCAGCCT TGTGGTCTCA CTTGTTACAT
    TACCAGGAAA ACAGGCAGAG GAGGCCAACG TCAGGAAGTT TAAGTACCTC AGGAATCCTT
    CTCGATTCAG AACGGAGGAA GTCTGATGCC AGTCCAGCGA TGTCTCCTCT AAGGGTGTCT
    CTCATTCAGG ATATGAGGCA CATCCAGAAC ATTGGAGAAA TCAAAACAGA TGTCGGGAAG
    GCCCGAGCCT GGGTCCGGCT GTCCATGGAA AAGAAGTTGC TTTCCAGACA CCTCAAACAG
    CTTCTTTCGG ATCATGAACT GACCAAGAAG CTGTACAAGC GTTACGCCTT TCTGCGCTGT
    GACGATGAGA AGGAGCAGTT CCTCTACCAC CTGCTCTCCT TCAATGCTGT CGATTACTTC
    TGCTTCACCA ACGTCTTCAC CACCATCATG ATCCCGTACC ACATCCTGAT CGTCCCAAGC
    AAGAAGCTCG GTGGGTCCAT GTTCACTGCC AATCCCTGGC TCTGCATTTC GGGCGAGCTG
    GGCGAGACAC AGATCCTGCA GATCCCCAGG AACGTCTTGG AGATGACCTT CGAGTGGCAG
    AACCTGGGGA AGCTTACCAC CGTCCAGATC GGGCACGACA ACTCAGGCCT CTATGCCAAG
    TGGCTCGTGG AGTATGTGAT GGTCAGAAAC GAAATCACGG GACACACGTA CAAATTCCCC
    TGTGGCCGGT GGCTGGGGAA GGGTACGGAC GATGGGAGTC TGGAGAGGGT CCTGGTCGGG
    GAGCTGCTCA CATCGCACCC TGAGGCAGAT GAGCGGCAGT CTCGGACCCC ACCCCTGCAG
    CAGTCCCCCA GCATGATTAG GAGACTGGTC ACCATCTCCC CCAACAACAA GCCAAAGTTG
    AACACGGGGC AGATTCAGGA GTCCATTGGG GAAGCGGTCA ATGGCATCGT GAAGCATTTC
    CACAAGCCTG AGAAAGAGAG AGGCAGCCTG ACCCTGCTGC TGTGTGGGGA GTGCGGGCTC
    GTGTCTGCCC TGGAACAAGT TTTTCAGCAC GGTTTTAAAT CGCCCCGGCT CTTCAAGAAC
    GTCTTCATCT GGGACTTCCT GGAAAAAGCA CAAACCTACT ATGAGACCCT GGACCAGAGT
    GAGATGGTCC CTGAGGAGAA CTGGCAGACA CGGGCCCGGA ACTTCTGCCG CTTCATCACA
    GCAATCAACA ACACCCCACG GAACATCGGC AAGGACGGAA AATTCCAGAC GCTGGTGTGC
    CTGGGTGCCA GAGACCACCT CCTCCACCAC TGGATCGCTT TGCTGGCGGA CTGCCCCATC
    ACGGCACAGA TGTATGAGGA CGTGGCATTG ATTAAGGACC ACACCCTTGT CAATTCCTTG
    ATCAGAGTGC TGCAGACCTT ACAGGAATTC AACATCACTC TGGAGACGTC GCTCGTCAAA
    GGGATTGATA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028916367.1
    CDS350..4264
    Translation

    Target ORF information:

    RefSeq Version XM_029060534.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029060534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GGGGAAAATT TTGAGCAGAC ACCTCTGAGG CGAACGTTCA AGTCCAAGGT TCTCGCCCGT
    TATCCTGACA ACGTGGAGTG GAATCCCTTT GACCAGGATG CAGTGGGAAT GCTGTGCATG
    CCCAAGGGGC TGGCATTCAA GACCCAGGCG GACCCCAGGG ACCCCCAGTT CCACTCTTTT
    ATCATCACCC GGGAAGACGG CTCGCGGACG TTCGGCTTCT CCCTGACGTT TTACGAGGAG
    GTGACCAGCA AGCAGATCTG CAGCGCCATG CAGACGCTCT ACCACATGCA CAATGCCGAG
    TACGACGTGT TCCACGTGGC CCCATCCGAT GGTGGGGAGG GCAGGGGGGA CTCCGAGGAC
    TGCGACGCCA CCCCCGGGAC CAAACTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC
    ACGCTCTACG TATCCAAGTG CATCTGCCTC ATCGCCCCCA TGTCCTTCAT GAAGGCCTGC
    AGGCGGGTAT TGGAGCAGCT CCACCAGGCA GTCACCTCGC CCCAGCCTCC ACCCCTCCCC
    CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TCCCTCCTCC CGGGCGGTCC
    CTGAAATTCT CGGGGGTCTA TGGACCCATC GTCTGCCAGA GGCCGAGCAC CGGCGAGTTG
    CCCCTCTTCG ACTTCCCCGT GAGGGAGGTT TTTGAGCTGC TCGGCGTGGA GAACGTACTC
    CAGCTCTTTA CCTGCGCCCT CTTGGAGTTT CAGATCCTGC TGTACTCACA GCATTACCAG
    AGGCTGATGA CAGTGGCGGA GACGATCACG GCCCTCATGT TTCCCTTCCA GTGGCAGCAC
    GTCTATGTGC CAATCCTCCC GGCCTCGCTT CTGCACTTCT TAGACGCTCC CGTCCCCTAC
    CTGATGGGCC TGCACTCCAA TGGCCTGGAT GACCGGTCCA AGCTGGAACT GCCTCAGGAG
    GCTAACCTGT GCTTTGTGGA CATAGACAAC CACTTTATTG AGCTGCCTGA AGACTTGCCT
    CAGTTTCCCA ACAAACTGGA GTTTGTCCAG GAGGTCTCCG AGATCCTCAT GGCATTTGGT
    ATCCCCCCGG AAGGGAACCT GCACTGCAGC GAAAGTGCCT CCAAGCTGAA GAGCCTAAGG
    GCCTGTGACA TTGTGTCGGA CAAGCGGAAC GGGAACCTTG CCAGCTCTCC TCTGAATTCC
    TATGAACTTC TCAAGAAAAA TGAGACCATC GCTCGGCTCC AAGCCCTGGT CAAAAGGACC
    GGGGTGAGCT TAGAAAAGCT TGACGTTCGG GAGGAGACCA GTAGCAATAA GGATCTCAAA
    GTCCAGTGCG ACGAAGAGGA GCTGAAGATT TACCAGCTGA ACATTCAAGT CCGGGAGGTC
    TTTGCAAACC GTTTCACTCA GATGTTTGCC GACTATGAGG TCTTTGTCAT CCAGCCCAGT
    CAAGATAAGG AGTCCTGGTT CACCAACAGG GAGCAGATGC AAAACTTTGA TAAAGCATCT
    TTCTTATCAG ACCAGCCCGA GCCCTACCTG CCCTTCCTTT CCCGGTTCCT GGAGACGCAG
    ATGTTTGCCT CCTTTGTGGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC
    GTGCTGCGGG TGTTTGATGC GCGGGTGGAC AAGATCAGGT TGCTAAATGT CAGGACCCCC
    ACCCTGCGGA CATCTATGTA CCAGAAGTGC ACCACTGTGG ATGAAGCAGA GAAGGCCATT
    GAGATGAGGC TGGCCAAGAT CGACCACACG GCTGTGCACC CCCACTTACT AGACATGAAG
    ATTGGGCAGG GCAAGTACGA GCCGGGATTC TTCCCCAAAC TGCAGCCGGA CGTGCTGTCC
    ACCGGCCCGG CCAGCAATAA GTGGACGAAG AGGAACGCAC CCGCCCAGTG GAGGCGGAAG
    GATCGGCAGA AGCAACACAC GGAGCATCTA CGCCTGGACA ACGATCAGAG GGAGCGGTTG
    GACTTTTCCG CTCCCAAGCT TCAGATGCAG TTTTATTTAG CACGGAGCCA GCCCGGGTGG
    TCTTTCAGGT CCTCTCTAAA ACCGATGTCC GACCTTTACC TGAAATACAT CCAGGAGGCC
    AGGAACCTTG GCAGCAACAT CCGCCAGCCC AAATTATCGA ACCTCTCACC ATCCGTGATT
    GCGCAGACCA ACTGGAAGTT TGTTGAGGGT TTACTGAAAG AATGCCGCAA CAAGACCAAG
    AGGATGCTGG TGGAGAAGAT GGGCCGGGAA GCCGTGGAGC TGGGCCACGG GGAGGTGAAC
    ATCACTGGCG TAGAAGAGAA CACTCTGATC GCCAGCCTCT GTGATCTGCT GGAGAGAATT
    TGGAGCCACG GCCTGCAGAT CAAGCAGGGT AAATCAGCCT TGTGGTCTCA CTTGTTACAT
    TACCAGGAAA ACAGGCAGAG GAGGCCAACG TCAGGAAGTT TAAGTACCTC AGGAATCCTT
    CTCGATTCAG AACGGAGGAA GTCTGATGCC AGTCCAGCGA TGTCTCCTCT AAGGGTGTCT
    CTCATTCAGG ATATGAGGCA CATCCAGAAC ATTGGAGAAA TCAAAACAGA TGTCGGGAAG
    GCCCGAGCCT GGGTCCGGCT GTCCATGGAA AAGAAGTTGC TTTCCAGACA CCTCAAACAG
    CTTCTTTCGG ATCATGAACT GACCAAGAAG CTGTACAAGC GTTACGCCTT TCTGCGCTGT
    GACGATGAGA AGGAGCAGTT CCTCTACCAC CTGCTCTCCT TCAATGCTGT CGATTACTTC
    TGCTTCACCA ACGTCTTCAC CACCATCATG ATCCCGTACC ACATCCTGAT CGTCCCAAGC
    AAGAAGCTCG GTGGGTCCAT GTTCACTGCC AATCCCTGGC TCTGCATTTC GGGCGAGCTG
    GGCGAGACAC AGATCCTGCA GATCCCCAGG AACGTCTTGG AGATGACCTT CGAGTGGCAG
    AACCTGGGGA AGCTTACCAC CGTCCAGATC GGGCACGACA ACTCAGGCCT CTATGCCAAG
    TGGCTCGTGG AGTATGTGAT GGTCAGAAAC GAAATCACGG GACACACGTA CAAATTCCCC
    TGTGGCCGGT GGCTGGGGAA GGGTACGGAC GATGGGAGTC TGGAGAGGGT CCTGGTCGGG
    GAGCTGCTCA CATCGCACCC TGAGGCAGAT GAGCGGCAGT CTCGGACCCC ACCCCTGCAG
    CAGTCCCCCA GCATGATTAG GAGACTGGTC ACCATCTCCC CCAACAACAA GCCAAAGTTG
    AACACGGGGC AGATTCAGGA GTCCATTGGG GAAGCGGTCA ATGGCATCGT GAAGCATTTC
    CACAAGCCTG AGAAAGAGAG AGGCAGCCTG ACCCTGCTGC TGTGTGGGGA GTGCGGGCTC
    GTGTCTGCCC TGGAACAAGT TTTTCAGCAC GGTTTTAAAT CGCCCCGGCT CTTCAAGAAC
    GTCTTCATCT GGGACTTCCT GGAAAAAGCA CAAACCTACT ATGAGACCCT GGACCAGAGT
    GAGATGGTCC CTGAGGAGAA CTGGCAGACA CGGGCCCGGA ACTTCTGCCG CTTCATCACA
    GCAATCAACA ACACCCCACG GAACATCGGC AAGGACGGAA AATTCCAGAC GCTGGTGTGC
    CTGGGTGCCA GAGACCACCT CCTCCACCAC TGGATCGCTT TGCTGGCGGA CTGCCCCATC
    ACGGCACAGA TGTATGAGGA CGTGGCATTG ATTAAGGACC ACACCCTTGT CAATTCCTTG
    ATCAGAGTGC TGCAGACCTT ACAGGAATTC AACATCACTC TGGAGACGTC GCTCGTCAAA
    GGGATTGATA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125855
    Clone ID Related Accession (Same CDS sequence) XM_029060535.1
    Accession Version XM_029060535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product DENN domain-containing protein 5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GCTTTATGCC AGTACATACA GGCTTCTAAA GCCAGAGGTG GTGCCAGCCC TTTCATTTCC
    AGTGCCGCAG AAGGGGAAAA TTTTGAGCAG ACACCTCTGA GGCGAACGTT CAAGTCCAAG
    GTTCTCGCCC GTTATCCTGA CAACGTGGAG TGGAATCCCT TTGACCAGGA TGCAGTGGGA
    ATGCTGTGCA TGCCCAAGGG GCTGGCATTC AAGACCCAGG CGGACCCCAG GGACCCCCAG
    TTCCACTCTT TTATCATCAC CCGGGAAGAC GGCTCGCGGA CGTTCGGCTT CTCCCTGACG
    TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGCGCCA TGCAGACGCT CTACCACATG
    CACAATGCCG AGTACGACGT GTTCCACGTG GCCCCATCCG ATGGTGGGGA GGGCAGGGGG
    GACTCCGAGG ACTGCGACGC CACCCCCGGG ACCAAACTGC AGCGCTTCAA CTCGTACGAC
    ATCAGCCGGG ACACGCTCTA CGTATCCAAG TGCATCTGCC TCATCGCCCC CATGTCCTTC
    ATGAAGGCCT GCAGGCGGGT ATTGGAGCAG CTCCACCAGG CAGTCACCTC GCCCCAGCCT
    CCACCCCTCC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTCCCTCCT
    CCCGGGCGGT CCCTGAAATT CTCGGGGGTC TATGGACCCA TCGTCTGCCA GAGGCCGAGC
    ACCGGCGAGT TGCCCCTCTT CGACTTCCCC GTGAGGGAGG TTTTTGAGCT GCTCGGCGTG
    GAGAACGTAC TCCAGCTCTT TACCTGCGCC CTCTTGGAGT TTCAGATCCT GCTGTACTCA
    CAGCATTACC AGAGGCTGAT GACAGTGGCG GAGACGATCA CGGCCCTCAT GTTTCCCTTC
    CAGTGGCAGC ACGTCTATGT GCCAATCCTC CCGGCCTCGC TTCTGCACTT CTTAGACGCT
    CCCGTCCCCT ACCTGATGGG CCTGCACTCC AATGGCCTGG ATGACCGGTC CAAGCTGGAA
    CTGCCTCAGG AGGCTAACCT GTGCTTTGTG GACATAGACA ACCACTTTAT TGAGCTGCCT
    GAAGACTTGC CTCAGTTTCC CAACAAACTG GAGTTTGTCC AGGAGGTCTC CGAGATCCTC
    ATGGCATTTG GTATCCCCCC GGAAGGGAAC CTGCACTGCA GCGAAAGTGC CTCCAAGCTG
    AAGAGCCTAA GGGCCTGTGA CATTGTGTCG GACAAGCGGA ACGGGAACCT TGCCAGCTCT
    CCTCTGAATT CCTATGAACT TCTCAAGAAA AATGAGACCA TCGCTCGGCT CCAAGCCCTG
    GTCAAAAGGA CCGGGGTGAG CTTAGAAAAG CTTGACGTTC GGGAGGAGAC CAGTAGCAAT
    AAGGATCTCA AAGTCCAGTG CGACGAAGAG GAGCTGAAGA TTTACCAGCT GAACATTCAA
    GTCCGGGAGG TCTTTGCAAA CCGTTTCACT CAGATGTTTG CCGACTATGA GGTCTTTGTC
    ATCCAGCCCA GTCAAGATAA GGAGTCCTGG TTCACCAACA GGGAGCAGAT GCAAAACTTT
    GATAAAGCAT CTTTCTTATC AGACCAGCCC GAGCCCTACC TGCCCTTCCT TTCCCGGTTC
    CTGGAGACGC AGATGTTTGC CTCCTTTGTG GACAATAAGA TCATGTGCCA CGATGATGAT
    GACAAAGACC CCGTGCTGCG GGTGTTTGAT GCGCGGGTGG ACAAGATCAG GTTGCTAAAT
    GTCAGGACCC CCACCCTGCG GACATCTATG TACCAGAAGT GCACCACTGT GGATGAAGCA
    GAGAAGGCCA TTGAGATGAG GCTGGCCAAG ATCGACCACA CGGCTGTGCA CCCCCACTTA
    CTAGACATGA AGATTGGGCA GGGCAAGTAC GAGCCGGGAT TCTTCCCCAA ACTGCAGCCG
    GACGTGCTGT CCACCGGCCC GGCCAGCAAT AAGTGGACGA AGAGGAACGC ACCCGCCCAG
    TGGAGGCGGA AGGATCGGCA GAAGCAACAC ACGGAGCATC TACGCCTGGA CAACGATCAG
    AGGGAGAAAT ACATCCAGGA GGCCAGGAAC CTTGGCAGCA ACATCCGCCA GCCCAAATTA
    TCGAACCTCT CACCATCCGT GATTGCGCAG ACCAACTGGA AGTTTGTTGA GGGTTTACTG
    AAAGAATGCC GCAACAAGAC CAAGAGGATG CTGGTGGAGA AGATGGGCCG GGAAGCCGTG
    GAGCTGGGCC ACGGGGAGGT GAACATCACT GGCGTAGAAG AGAACACTCT GATCGCCAGC
    CTCTGTGATC TGCTGGAGAG AATTTGGAGC CACGGCCTGC AGATCAAGCA GGGTAAATCA
    GCCTTGTGGT CTCACTTGTT ACATTACCAG GAAAACAGGC AGAGGAGGCC AACGTCAGGA
    AGTTTAAGTA CCTCAGGAAT CCTTCTCGAT TCAGAACGGA GGAAGTCTGA TGCCAGTCCA
    GCGATGTCTC CTCTAAGGGT GTCTCTCATT CAGGATATGA GGCACATCCA GAACATTGGA
    GAAATCAAAA CAGATGTCGG GAAGGCCCGA GCCTGGGTCC GGCTGTCCAT GGAAAAGAAG
    TTGCTTTCCA GACACCTCAA ACAGCTTCTT TCGGATCATG AACTGACCAA GAAGCTGTAC
    AAGCGTTACG CCTTTCTGCG CTGTGACGAT GAGAAGGAGC AGTTCCTCTA CCACCTGCTC
    TCCTTCAATG CTGTCGATTA CTTCTGCTTC ACCAACGTCT TCACCACCAT CATGATCCCG
    TACCACATCC TGATCGTCCC AAGCAAGAAG CTCGGTGGGT CCATGTTCAC TGCCAATCCC
    TGGCTCTGCA TTTCGGGCGA GCTGGGCGAG ACACAGATCC TGCAGATCCC CAGGAACGTC
    TTGGAGATGA CCTTCGAGTG GCAGAACCTG GGGAAGCTTA CCACCGTCCA GATCGGGCAC
    GACAACTCAG GCCTCTATGC CAAGTGGCTC GTGGAGTATG TGATGGTCAG AAACGAAATC
    ACGGGACACA CGTACAAATT CCCCTGTGGC CGGTGGCTGG GGAAGGGTAC GGACGATGGG
    AGTCTGGAGA GGGTCCTGGT CGGGGAGCTG CTCACATCGC ACCCTGAGGC AGATGAGCGG
    CAGTCTCGGA CCCCACCCCT GCAGCAGTCC CCCAGCATGA TTAGGAGACT GGTCACCATC
    TCCCCCAACA ACAAGCCAAA GTTGAACACG GGGCAGATTC AGGAGTCCAT TGGGGAAGCG
    GTCAATGGCA TCGTGAAGCA TTTCCACAAG CCTGAGAAAG AGAGAGGCAG CCTGACCCTG
    CTGCTGTGTG GGGAGTGCGG GCTCGTGTCT GCCCTGGAAC AAGTTTTTCA GCACGGTTTT
    AAATCGCCCC GGCTCTTCAA GAACGTCTTC ATCTGGGACT TCCTGGAAAA AGCACAAACC
    TACTATGAGA CCCTGGACCA GAGTGAGATG GTCCCTGAGG AGAACTGGCA GACACGGGCC
    CGGAACTTCT GCCGCTTCAT CACAGCAATC AACAACACCC CACGGAACAT CGGCAAGGAC
    GGAAAATTCC AGACGCTGGT GTGCCTGGGT GCCAGAGACC ACCTCCTCCA CCACTGGATC
    GCTTTGCTGG CGGACTGCCC CATCACGGCA CAGATGTATG AGGACGTGGC ATTGATTAAG
    GACCACACCC TTGTCAATTC CTTGATCAGA GTGCTGCAGA CCTTACAGGA ATTCAACATC
    ACTCTGGAGA CGTCGCTCGT CAAAGGGATT GATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028916368.1
    CDS350..4228
    Translation

    Target ORF information:

    RefSeq Version XM_029060535.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029060535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GCTTTATGCC AGTACATACA GGCTTCTAAA GCCAGAGGTG GTGCCAGCCC TTTCATTTCC
    AGTGCCGCAG AAGGGGAAAA TTTTGAGCAG ACACCTCTGA GGCGAACGTT CAAGTCCAAG
    GTTCTCGCCC GTTATCCTGA CAACGTGGAG TGGAATCCCT TTGACCAGGA TGCAGTGGGA
    ATGCTGTGCA TGCCCAAGGG GCTGGCATTC AAGACCCAGG CGGACCCCAG GGACCCCCAG
    TTCCACTCTT TTATCATCAC CCGGGAAGAC GGCTCGCGGA CGTTCGGCTT CTCCCTGACG
    TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGCGCCA TGCAGACGCT CTACCACATG
    CACAATGCCG AGTACGACGT GTTCCACGTG GCCCCATCCG ATGGTGGGGA GGGCAGGGGG
    GACTCCGAGG ACTGCGACGC CACCCCCGGG ACCAAACTGC AGCGCTTCAA CTCGTACGAC
    ATCAGCCGGG ACACGCTCTA CGTATCCAAG TGCATCTGCC TCATCGCCCC CATGTCCTTC
    ATGAAGGCCT GCAGGCGGGT ATTGGAGCAG CTCCACCAGG CAGTCACCTC GCCCCAGCCT
    CCACCCCTCC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTCCCTCCT
    CCCGGGCGGT CCCTGAAATT CTCGGGGGTC TATGGACCCA TCGTCTGCCA GAGGCCGAGC
    ACCGGCGAGT TGCCCCTCTT CGACTTCCCC GTGAGGGAGG TTTTTGAGCT GCTCGGCGTG
    GAGAACGTAC TCCAGCTCTT TACCTGCGCC CTCTTGGAGT TTCAGATCCT GCTGTACTCA
    CAGCATTACC AGAGGCTGAT GACAGTGGCG GAGACGATCA CGGCCCTCAT GTTTCCCTTC
    CAGTGGCAGC ACGTCTATGT GCCAATCCTC CCGGCCTCGC TTCTGCACTT CTTAGACGCT
    CCCGTCCCCT ACCTGATGGG CCTGCACTCC AATGGCCTGG ATGACCGGTC CAAGCTGGAA
    CTGCCTCAGG AGGCTAACCT GTGCTTTGTG GACATAGACA ACCACTTTAT TGAGCTGCCT
    GAAGACTTGC CTCAGTTTCC CAACAAACTG GAGTTTGTCC AGGAGGTCTC CGAGATCCTC
    ATGGCATTTG GTATCCCCCC GGAAGGGAAC CTGCACTGCA GCGAAAGTGC CTCCAAGCTG
    AAGAGCCTAA GGGCCTGTGA CATTGTGTCG GACAAGCGGA ACGGGAACCT TGCCAGCTCT
    CCTCTGAATT CCTATGAACT TCTCAAGAAA AATGAGACCA TCGCTCGGCT CCAAGCCCTG
    GTCAAAAGGA CCGGGGTGAG CTTAGAAAAG CTTGACGTTC GGGAGGAGAC CAGTAGCAAT
    AAGGATCTCA AAGTCCAGTG CGACGAAGAG GAGCTGAAGA TTTACCAGCT GAACATTCAA
    GTCCGGGAGG TCTTTGCAAA CCGTTTCACT CAGATGTTTG CCGACTATGA GGTCTTTGTC
    ATCCAGCCCA GTCAAGATAA GGAGTCCTGG TTCACCAACA GGGAGCAGAT GCAAAACTTT
    GATAAAGCAT CTTTCTTATC AGACCAGCCC GAGCCCTACC TGCCCTTCCT TTCCCGGTTC
    CTGGAGACGC AGATGTTTGC CTCCTTTGTG GACAATAAGA TCATGTGCCA CGATGATGAT
    GACAAAGACC CCGTGCTGCG GGTGTTTGAT GCGCGGGTGG ACAAGATCAG GTTGCTAAAT
    GTCAGGACCC CCACCCTGCG GACATCTATG TACCAGAAGT GCACCACTGT GGATGAAGCA
    GAGAAGGCCA TTGAGATGAG GCTGGCCAAG ATCGACCACA CGGCTGTGCA CCCCCACTTA
    CTAGACATGA AGATTGGGCA GGGCAAGTAC GAGCCGGGAT TCTTCCCCAA ACTGCAGCCG
    GACGTGCTGT CCACCGGCCC GGCCAGCAAT AAGTGGACGA AGAGGAACGC ACCCGCCCAG
    TGGAGGCGGA AGGATCGGCA GAAGCAACAC ACGGAGCATC TACGCCTGGA CAACGATCAG
    AGGGAGAAAT ACATCCAGGA GGCCAGGAAC CTTGGCAGCA ACATCCGCCA GCCCAAATTA
    TCGAACCTCT CACCATCCGT GATTGCGCAG ACCAACTGGA AGTTTGTTGA GGGTTTACTG
    AAAGAATGCC GCAACAAGAC CAAGAGGATG CTGGTGGAGA AGATGGGCCG GGAAGCCGTG
    GAGCTGGGCC ACGGGGAGGT GAACATCACT GGCGTAGAAG AGAACACTCT GATCGCCAGC
    CTCTGTGATC TGCTGGAGAG AATTTGGAGC CACGGCCTGC AGATCAAGCA GGGTAAATCA
    GCCTTGTGGT CTCACTTGTT ACATTACCAG GAAAACAGGC AGAGGAGGCC AACGTCAGGA
    AGTTTAAGTA CCTCAGGAAT CCTTCTCGAT TCAGAACGGA GGAAGTCTGA TGCCAGTCCA
    GCGATGTCTC CTCTAAGGGT GTCTCTCATT CAGGATATGA GGCACATCCA GAACATTGGA
    GAAATCAAAA CAGATGTCGG GAAGGCCCGA GCCTGGGTCC GGCTGTCCAT GGAAAAGAAG
    TTGCTTTCCA GACACCTCAA ACAGCTTCTT TCGGATCATG AACTGACCAA GAAGCTGTAC
    AAGCGTTACG CCTTTCTGCG CTGTGACGAT GAGAAGGAGC AGTTCCTCTA CCACCTGCTC
    TCCTTCAATG CTGTCGATTA CTTCTGCTTC ACCAACGTCT TCACCACCAT CATGATCCCG
    TACCACATCC TGATCGTCCC AAGCAAGAAG CTCGGTGGGT CCATGTTCAC TGCCAATCCC
    TGGCTCTGCA TTTCGGGCGA GCTGGGCGAG ACACAGATCC TGCAGATCCC CAGGAACGTC
    TTGGAGATGA CCTTCGAGTG GCAGAACCTG GGGAAGCTTA CCACCGTCCA GATCGGGCAC
    GACAACTCAG GCCTCTATGC CAAGTGGCTC GTGGAGTATG TGATGGTCAG AAACGAAATC
    ACGGGACACA CGTACAAATT CCCCTGTGGC CGGTGGCTGG GGAAGGGTAC GGACGATGGG
    AGTCTGGAGA GGGTCCTGGT CGGGGAGCTG CTCACATCGC ACCCTGAGGC AGATGAGCGG
    CAGTCTCGGA CCCCACCCCT GCAGCAGTCC CCCAGCATGA TTAGGAGACT GGTCACCATC
    TCCCCCAACA ACAAGCCAAA GTTGAACACG GGGCAGATTC AGGAGTCCAT TGGGGAAGCG
    GTCAATGGCA TCGTGAAGCA TTTCCACAAG CCTGAGAAAG AGAGAGGCAG CCTGACCCTG
    CTGCTGTGTG GGGAGTGCGG GCTCGTGTCT GCCCTGGAAC AAGTTTTTCA GCACGGTTTT
    AAATCGCCCC GGCTCTTCAA GAACGTCTTC ATCTGGGACT TCCTGGAAAA AGCACAAACC
    TACTATGAGA CCCTGGACCA GAGTGAGATG GTCCCTGAGG AGAACTGGCA GACACGGGCC
    CGGAACTTCT GCCGCTTCAT CACAGCAATC AACAACACCC CACGGAACAT CGGCAAGGAC
    GGAAAATTCC AGACGCTGGT GTGCCTGGGT GCCAGAGACC ACCTCCTCCA CCACTGGATC
    GCTTTGCTGG CGGACTGCCC CATCACGGCA CAGATGTATG AGGACGTGGC ATTGATTAAG
    GACCACACCC TTGTCAATTC CTTGATCAGA GTGCTGCAGA CCTTACAGGA ATTCAACATC
    ACTCTGGAGA CGTCGCTCGT CAAAGGGATT GATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOc125856
    Clone ID Related Accession (Same CDS sequence) XM_029060536.1
    Accession Version XM_029060536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-28
    Organism Ornithorhynchus anatinus(platypus)
    Product DENN domain-containing protein 5A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GGGGAAAATT TTGAGCAGAC ACCTCTGAGG CGAACGTTCA AGTCCAAGGT TCTCGCCCGT
    TATCCTGACA ACGTGGAGTG GAATCCCTTT GACCAGGATG CAGTGGGAAT GCTGTGCATG
    CCCAAGGGGC TGGCATTCAA GACCCAGGCG GACCCCAGGG ACCCCCAGTT CCACTCTTTT
    ATCATCACCC GGGAAGACGG CTCGCGGACG TTCGGCTTCT CCCTGACGTT TTACGAGGAG
    GTGACCAGCA AGCAGATCTG CAGCGCCATG CAGACGCTCT ACCACATGCA CAATGCCGAG
    TACGACGTGT TCCACGTGGC CCCATCCGAT GGTGGGGAGG GCAGGGGGGA CTCCGAGGAC
    TGCGACGCCA CCCCCGGGAC CAAACTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC
    ACGCTCTACG TATCCAAGTG CATCTGCCTC ATCGCCCCCA TGTCCTTCAT GAAGGCCTGC
    AGGCGGGTAT TGGAGCAGCT CCACCAGGCA GTCACCTCGC CCCAGCCTCC ACCCCTCCCC
    CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TCCCTCCTCC CGGGCGGTCC
    CTGAAATTCT CGGGGGTCTA TGGACCCATC GTCTGCCAGA GGCCGAGCAC CGGCGAGTTG
    CCCCTCTTCG ACTTCCCCGT GAGGGAGGTT TTTGAGCTGC TCGGCGTGGA GAACGTACTC
    CAGCTCTTTA CCTGCGCCCT CTTGGAGTTT CAGATCCTGC TGTACTCACA GCATTACCAG
    AGGCTGATGA CAGTGGCGGA GACGATCACG GCCCTCATGT TTCCCTTCCA GTGGCAGCAC
    GTCTATGTGC CAATCCTCCC GGCCTCGCTT CTGCACTTCT TAGACGCTCC CGTCCCCTAC
    CTGATGGGCC TGCACTCCAA TGGCCTGGAT GACCGGTCCA AGCTGGAACT GCCTCAGGAG
    GCTAACCTGT GCTTTGTGGA CATAGACAAC CACTTTATTG AGCTGCCTGA AGACTTGCCT
    CAGTTTCCCA ACAAACTGGA GTTTGTCCAG GAGGTCTCCG AGATCCTCAT GGCATTTGGT
    ATCCCCCCGG AAGGGAACCT GCACTGCAGC GAAAGTGCCT CCAAGCTGAA GAGCCTAAGG
    GCCTGTGACA TTGTGTCGGA CAAGCGGAAC GGGAACCTTG CCAGCTCTCC TCTGAATTCC
    TATGAACTTC TCAAGAAAAA TGAGACCATC GCTCGGCTCC AAGCCCTGGT CAAAAGGACC
    GGGGTGAGCT TAGAAAAGCT TGACGTTCGG GAGGAGACCA GTAGCAATAA GGATCTCAAA
    GTCCAGTGCG ACGAAGAGGA GCTGAAGATT TACCAGCTGA ACATTCAAGT CCGGGAGGTC
    TTTGCAAACC GTTTCACTCA GATGTTTGCC GACTATGAGG TCTTTGTCAT CCAGCCCAGT
    CAAGATAAGG AGTCCTGGTT CACCAACAGG GAGCAGATGC AAAACTTTGA TAAAGCATCT
    TTCTTATCAG ACCAGCCCGA GCCCTACCTG CCCTTCCTTT CCCGGTTCCT GGAGACGCAG
    ATGTTTGCCT CCTTTGTGGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC
    GTGCTGCGGG TGTTTGATGC GCGGGTGGAC AAGATCAGGT TGCTAAATGT CAGGACCCCC
    ACCCTGCGGA CATCTATGTA CCAGAAGTGC ACCACTGTGG ATGAAGCAGA GAAGGCCATT
    GAGATGAGGC TGGCCAAGAT CGACCACACG GCTGTGCACC CCCACTTACT AGACATGAAG
    ATTGGGCAGG GCAAGTACGA GCCGGGATTC TTCCCCAAAC TGCAGCCGGA CGTGCTGTCC
    ACCGGCCCGG CCAGCAATAA GTGGACGAAG AGGAACGCAC CCGCCCAGTG GAGGCGGAAG
    GATCGGCAGA AGCAACACAC GGAGCATCTA CGCCTGGACA ACGATCAGAG GGAGAAATAC
    ATCCAGGAGG CCAGGAACCT TGGCAGCAAC ATCCGCCAGC CCAAATTATC GAACCTCTCA
    CCATCCGTGA TTGCGCAGAC CAACTGGAAG TTTGTTGAGG GTTTACTGAA AGAATGCCGC
    AACAAGACCA AGAGGATGCT GGTGGAGAAG ATGGGCCGGG AAGCCGTGGA GCTGGGCCAC
    GGGGAGGTGA ACATCACTGG CGTAGAAGAG AACACTCTGA TCGCCAGCCT CTGTGATCTG
    CTGGAGAGAA TTTGGAGCCA CGGCCTGCAG ATCAAGCAGG GTAAATCAGC CTTGTGGTCT
    CACTTGTTAC ATTACCAGGA AAACAGGCAG AGGAGGCCAA CGTCAGGAAG TTTAAGTACC
    TCAGGAATCC TTCTCGATTC AGAACGGAGG AAGTCTGATG CCAGTCCAGC GATGTCTCCT
    CTAAGGGTGT CTCTCATTCA GGATATGAGG CACATCCAGA ACATTGGAGA AATCAAAACA
    GATGTCGGGA AGGCCCGAGC CTGGGTCCGG CTGTCCATGG AAAAGAAGTT GCTTTCCAGA
    CACCTCAAAC AGCTTCTTTC GGATCATGAA CTGACCAAGA AGCTGTACAA GCGTTACGCC
    TTTCTGCGCT GTGACGATGA GAAGGAGCAG TTCCTCTACC ACCTGCTCTC CTTCAATGCT
    GTCGATTACT TCTGCTTCAC CAACGTCTTC ACCACCATCA TGATCCCGTA CCACATCCTG
    ATCGTCCCAA GCAAGAAGCT CGGTGGGTCC ATGTTCACTG CCAATCCCTG GCTCTGCATT
    TCGGGCGAGC TGGGCGAGAC ACAGATCCTG CAGATCCCCA GGAACGTCTT GGAGATGACC
    TTCGAGTGGC AGAACCTGGG GAAGCTTACC ACCGTCCAGA TCGGGCACGA CAACTCAGGC
    CTCTATGCCA AGTGGCTCGT GGAGTATGTG ATGGTCAGAA ACGAAATCAC GGGACACACG
    TACAAATTCC CCTGTGGCCG GTGGCTGGGG AAGGGTACGG ACGATGGGAG TCTGGAGAGG
    GTCCTGGTCG GGGAGCTGCT CACATCGCAC CCTGAGGCAG ATGAGCGGCA GTCTCGGACC
    CCACCCCTGC AGCAGTCCCC CAGCATGATT AGGAGACTGG TCACCATCTC CCCCAACAAC
    AAGCCAAAGT TGAACACGGG GCAGATTCAG GAGTCCATTG GGGAAGCGGT CAATGGCATC
    GTGAAGCATT TCCACAAGCC TGAGAAAGAG AGAGGCAGCC TGACCCTGCT GCTGTGTGGG
    GAGTGCGGGC TCGTGTCTGC CCTGGAACAA GTTTTTCAGC ACGGTTTTAA ATCGCCCCGG
    CTCTTCAAGA ACGTCTTCAT CTGGGACTTC CTGGAAAAAG CACAAACCTA CTATGAGACC
    CTGGACCAGA GTGAGATGGT CCCTGAGGAG AACTGGCAGA CACGGGCCCG GAACTTCTGC
    CGCTTCATCA CAGCAATCAA CAACACCCCA CGGAACATCG GCAAGGACGG AAAATTCCAG
    ACGCTGGTGT GCCTGGGTGC CAGAGACCAC CTCCTCCACC ACTGGATCGC TTTGCTGGCG
    GACTGCCCCA TCACGGCACA GATGTATGAG GACGTGGCAT TGATTAAGGA CCACACCCTT
    GTCAATTCCT TGATCAGAGT GCTGCAGACC TTACAGGAAT TCAACATCAC TCTGGAGACG
    TCGCTCGTCA AAGGGATTGA TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_028916369.1
    CDS350..4156
    Translation

    Target ORF information:

    RefSeq Version XM_029060536.1
    Organism Ornithorhynchus anatinus(platypus)
    Definition Ornithorhynchus anatinus DENN domain containing 5A (DENND5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_029060536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCC CCGGTGGGGG CGGTGGGGGC AGCAGCAGCT CAGCCCCCAG CCGCTTCGCC 
    GATTACTTTG TCATCTGCGG GCTGGACACC GACACCGGGC TGGAGCCGGA CGAGCTGTCG
    GGGGAAAATT TTGAGCAGAC ACCTCTGAGG CGAACGTTCA AGTCCAAGGT TCTCGCCCGT
    TATCCTGACA ACGTGGAGTG GAATCCCTTT GACCAGGATG CAGTGGGAAT GCTGTGCATG
    CCCAAGGGGC TGGCATTCAA GACCCAGGCG GACCCCAGGG ACCCCCAGTT CCACTCTTTT
    ATCATCACCC GGGAAGACGG CTCGCGGACG TTCGGCTTCT CCCTGACGTT TTACGAGGAG
    GTGACCAGCA AGCAGATCTG CAGCGCCATG CAGACGCTCT ACCACATGCA CAATGCCGAG
    TACGACGTGT TCCACGTGGC CCCATCCGAT GGTGGGGAGG GCAGGGGGGA CTCCGAGGAC
    TGCGACGCCA CCCCCGGGAC CAAACTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC
    ACGCTCTACG TATCCAAGTG CATCTGCCTC ATCGCCCCCA TGTCCTTCAT GAAGGCCTGC
    AGGCGGGTAT TGGAGCAGCT CCACCAGGCA GTCACCTCGC CCCAGCCTCC ACCCCTCCCC
    CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TCCCTCCTCC CGGGCGGTCC
    CTGAAATTCT CGGGGGTCTA TGGACCCATC GTCTGCCAGA GGCCGAGCAC CGGCGAGTTG
    CCCCTCTTCG ACTTCCCCGT GAGGGAGGTT TTTGAGCTGC TCGGCGTGGA GAACGTACTC
    CAGCTCTTTA CCTGCGCCCT CTTGGAGTTT CAGATCCTGC TGTACTCACA GCATTACCAG
    AGGCTGATGA CAGTGGCGGA GACGATCACG GCCCTCATGT TTCCCTTCCA GTGGCAGCAC
    GTCTATGTGC CAATCCTCCC GGCCTCGCTT CTGCACTTCT TAGACGCTCC CGTCCCCTAC
    CTGATGGGCC TGCACTCCAA TGGCCTGGAT GACCGGTCCA AGCTGGAACT GCCTCAGGAG
    GCTAACCTGT GCTTTGTGGA CATAGACAAC CACTTTATTG AGCTGCCTGA AGACTTGCCT
    CAGTTTCCCA ACAAACTGGA GTTTGTCCAG GAGGTCTCCG AGATCCTCAT GGCATTTGGT
    ATCCCCCCGG AAGGGAACCT GCACTGCAGC GAAAGTGCCT CCAAGCTGAA GAGCCTAAGG
    GCCTGTGACA TTGTGTCGGA CAAGCGGAAC GGGAACCTTG CCAGCTCTCC TCTGAATTCC
    TATGAACTTC TCAAGAAAAA TGAGACCATC GCTCGGCTCC AAGCCCTGGT CAAAAGGACC
    GGGGTGAGCT TAGAAAAGCT TGACGTTCGG GAGGAGACCA GTAGCAATAA GGATCTCAAA
    GTCCAGTGCG ACGAAGAGGA GCTGAAGATT TACCAGCTGA ACATTCAAGT CCGGGAGGTC
    TTTGCAAACC GTTTCACTCA GATGTTTGCC GACTATGAGG TCTTTGTCAT CCAGCCCAGT
    CAAGATAAGG AGTCCTGGTT CACCAACAGG GAGCAGATGC AAAACTTTGA TAAAGCATCT
    TTCTTATCAG ACCAGCCCGA GCCCTACCTG CCCTTCCTTT CCCGGTTCCT GGAGACGCAG
    ATGTTTGCCT CCTTTGTGGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC
    GTGCTGCGGG TGTTTGATGC GCGGGTGGAC AAGATCAGGT TGCTAAATGT CAGGACCCCC
    ACCCTGCGGA CATCTATGTA CCAGAAGTGC ACCACTGTGG ATGAAGCAGA GAAGGCCATT
    GAGATGAGGC TGGCCAAGAT CGACCACACG GCTGTGCACC CCCACTTACT AGACATGAAG
    ATTGGGCAGG GCAAGTACGA GCCGGGATTC TTCCCCAAAC TGCAGCCGGA CGTGCTGTCC
    ACCGGCCCGG CCAGCAATAA GTGGACGAAG AGGAACGCAC CCGCCCAGTG GAGGCGGAAG
    GATCGGCAGA AGCAACACAC GGAGCATCTA CGCCTGGACA ACGATCAGAG GGAGAAATAC
    ATCCAGGAGG CCAGGAACCT TGGCAGCAAC ATCCGCCAGC CCAAATTATC GAACCTCTCA
    CCATCCGTGA TTGCGCAGAC CAACTGGAAG TTTGTTGAGG GTTTACTGAA AGAATGCCGC
    AACAAGACCA AGAGGATGCT GGTGGAGAAG ATGGGCCGGG AAGCCGTGGA GCTGGGCCAC
    GGGGAGGTGA ACATCACTGG CGTAGAAGAG AACACTCTGA TCGCCAGCCT CTGTGATCTG
    CTGGAGAGAA TTTGGAGCCA CGGCCTGCAG ATCAAGCAGG GTAAATCAGC CTTGTGGTCT
    CACTTGTTAC ATTACCAGGA AAACAGGCAG AGGAGGCCAA CGTCAGGAAG TTTAAGTACC
    TCAGGAATCC TTCTCGATTC AGAACGGAGG AAGTCTGATG CCAGTCCAGC GATGTCTCCT
    CTAAGGGTGT CTCTCATTCA GGATATGAGG CACATCCAGA ACATTGGAGA AATCAAAACA
    GATGTCGGGA AGGCCCGAGC CTGGGTCCGG CTGTCCATGG AAAAGAAGTT GCTTTCCAGA
    CACCTCAAAC AGCTTCTTTC GGATCATGAA CTGACCAAGA AGCTGTACAA GCGTTACGCC
    TTTCTGCGCT GTGACGATGA GAAGGAGCAG TTCCTCTACC ACCTGCTCTC CTTCAATGCT
    GTCGATTACT TCTGCTTCAC CAACGTCTTC ACCACCATCA TGATCCCGTA CCACATCCTG
    ATCGTCCCAA GCAAGAAGCT CGGTGGGTCC ATGTTCACTG CCAATCCCTG GCTCTGCATT
    TCGGGCGAGC TGGGCGAGAC ACAGATCCTG CAGATCCCCA GGAACGTCTT GGAGATGACC
    TTCGAGTGGC AGAACCTGGG GAAGCTTACC ACCGTCCAGA TCGGGCACGA CAACTCAGGC
    CTCTATGCCA AGTGGCTCGT GGAGTATGTG ATGGTCAGAA ACGAAATCAC GGGACACACG
    TACAAATTCC CCTGTGGCCG GTGGCTGGGG AAGGGTACGG ACGATGGGAG TCTGGAGAGG
    GTCCTGGTCG GGGAGCTGCT CACATCGCAC CCTGAGGCAG ATGAGCGGCA GTCTCGGACC
    CCACCCCTGC AGCAGTCCCC CAGCATGATT AGGAGACTGG TCACCATCTC CCCCAACAAC
    AAGCCAAAGT TGAACACGGG GCAGATTCAG GAGTCCATTG GGGAAGCGGT CAATGGCATC
    GTGAAGCATT TCCACAAGCC TGAGAAAGAG AGAGGCAGCC TGACCCTGCT GCTGTGTGGG
    GAGTGCGGGC TCGTGTCTGC CCTGGAACAA GTTTTTCAGC ACGGTTTTAA ATCGCCCCGG
    CTCTTCAAGA ACGTCTTCAT CTGGGACTTC CTGGAAAAAG CACAAACCTA CTATGAGACC
    CTGGACCAGA GTGAGATGGT CCCTGAGGAG AACTGGCAGA CACGGGCCCG GAACTTCTGC
    CGCTTCATCA CAGCAATCAA CAACACCCCA CGGAACATCG GCAAGGACGG AAAATTCCAG
    ACGCTGGTGT GCCTGGGTGC CAGAGACCAC CTCCTCCACC ACTGGATCGC TTTGCTGGCG
    GACTGCCCCA TCACGGCACA GATGTATGAG GACGTGGCAT TGATTAAGGA CCACACCCTT
    GTCAATTCCT TGATCAGAGT GCTGCAGACC TTACAGGAAT TCAACATCAC TCTGGAGACG
    TCGCTCGTCA AAGGGATTGA TATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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