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BUB1B cDNA ORF clone, Pan troglodytes(chimpanzee)

Summary

Gene Symbol BUB1B
Entrez Gene ID 453324
Full Name BUB1 mitotic checkpoint serine/threonine kinase B
General protein information
Preferred Names

BUB1 mitotic checkpoint serine/threonine kinase B

Names

mitotic checkpoint serine/threonine-protein kinase BUB1 beta
BUB1 budding uninhibited by benzimidazoles 1 homolog beta
budding uninhibited by benzimidazoles 1 homolog beta
Gene Type protein-coding
Organism Pan troglodytes(chimpanzee)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_009428789.2 XP_009427064.2 mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X3
XM_009428788.2 XP_009427063.2 mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
XM_009428788.3 XP_009427063.2 mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
XM_009428789.3 XP_009427064.2 mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X3
XM_510299.5 XP_510299.4 mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) BUB1B NP_001202.4
Pan troglodytes (chimpanzee) BUB1B XP_510299.3
BUB1B XP_002804771.1
Canis lupus familiaris (dog) BUB1B XP_850443.2
Gallus gallus (chicken) BUB1B NP_989734.1
Bos taurus (cattle) BUB1B NP_001138645.1
Arabidopsis thaliana (thale cress) BUBR1 NP_565767.1
Oryza sativa (rice) Os02g0193600 NP_001046171.1
Mus musculus (house mouse) Bub1b NP_033903.2
Rattus norvegicus (Norway rat) Bub1b XP_342495.4
Danio rerio (zebrafish) bub1bb XP_695384.3
Xenopus tropicalis (tropical clawed frog) bub1b NP_001096484.1

Gene Ontology

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc69471 XM_009428788.3
Latest version!		 Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
OPc69472 XM_510299.5
Latest version!		 Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
OPc69473 XM_009428789.3
Latest version!		 Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OPc69471 XM_009428788.2 Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
OPc69473 XM_009428789.2 Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OPc69471
Clone ID Related Accession (Same CDS sequence) XM_009428788.2 , XM_009428788.3
Accession Version XM_009428788.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3168bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-01
Organism Pan troglodytes(chimpanzee)
Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGTCT CTCTTTCTCT AGGTCCCAGT GTACCTTTCT CCATTTTTGA TGAGTTTCTT 
CTTTCAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009427063.2
CDS208..3375
Translation

Target ORF information:

RefSeq Version XM_009428788.2
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009428788.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGTCT CTCTTTCTCT AGGTCCCAGT GTACCTTTCT CCATTTTTGA TGAGTTTCTT 
CTTTCAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc69471
Clone ID Related Accession (Same CDS sequence) XM_009428788.2 , XM_009428788.3
Accession Version XM_009428788.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3168bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-19
Organism Pan troglodytes(chimpanzee)
Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGTCT CTCTTTCTCT AGGTCCCAGT GTACCTTTCT CCATTTTTGA TGAGTTTCTT 
CTTTCAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009427063.2
CDS208..3375
Translation

Target ORF information:

RefSeq Version XM_009428788.3
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009428788.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGTCT CTCTTTCTCT AGGTCCCAGT GTACCTTTCT CCATTTTTGA TGAGTTTCTT 
CTTTCAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc69472
Clone ID Related Accession (Same CDS sequence) XM_510299.5
Accession Version XM_510299.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3153bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-19
Organism Pan troglodytes(chimpanzee)
Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_510299.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAGGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTATAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCG 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_510299.4
CDS213..3365
Translation

Target ORF information:

RefSeq Version XM_510299.5
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_510299.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAGGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTATAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCG 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc69473
Clone ID Related Accession (Same CDS sequence) XM_009428789.2 , XM_009428789.3
Accession Version XM_009428789.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-01
Organism Pan troglodytes(chimpanzee)
Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006482.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009428789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009427064.2
CDS208..3315
Translation

Target ORF information:

RefSeq Version XM_009428789.2
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009428789.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc69473
Clone ID Related Accession (Same CDS sequence) XM_009428789.2 , XM_009428789.3
Accession Version XM_009428789.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-19
Organism Pan troglodytes(chimpanzee)
Product mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009428789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009427064.2
CDS208..3315
Translation

Target ORF information:

RefSeq Version XM_009428789.3
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009428789.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA GGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAGTAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGA AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA GACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGCCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGAAA AGAAGAATAA AAGTCCTCCT GCAGATCCCC CACGAGTTTT AGCTCAACGA 
AGACCCCTTG CAGTTCTCAA AACCTCAGAA AGCATCACCT CAAATGAAGA TGTGTCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAA CCCTTGAGCG AGGATGCCAT TATCACAGGC 
TTCAGAAATG TAACAATTTG TCCTAACCCA GAAGACACTT GTGACTTTGC CAGAGCAGCT 
CGTTTTGTAT CCACTCCTTT TCATGAGATA ATGTCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TACCGGAAGA GGATCTAGAT GTAAAGACCT CTGAGGACCA GCAGACAGCT 
TGTGGCACTA TCTACAGTCA GACTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGTCGTGAAG CCACACACTC CTCTGGCTTC TCTGGTTCTT CTGCCTCGGT TGCAAGCACC 
TCCTCCATCA AATGTCTTCA AATTCCTGAG AAACTAGAAC TTACTAATGA GACTTCAGAA 
AACCCTACTC AGTCACCATG GTGTTCACAG TATCGCAGAC AGCTACTGAA GTCCCTACCA 
GAGTTAAGTG CCTCTGCAGA GTTGTGTATA GAAGACAGAC CAATGCCTAA GTTGGAAATT 
GAGAAGGAAA TTGAATTAGG TAATGAGGAT TACTGCATTA AACGAGAATA CCTAATATGT 
GAAGATTACA AGTTATTCTG GGTGGCGCCA AGAAACTCTG CAGAATTAAC AGTAATAAAG 
GTATCTTCTC AACCTGTCCC ATGGGACTTT TATATCAACC TCAAGTTAAA GGAACGTTTA 
AATGAAGATT TTGATCATTT TTGCAGCTGT TATCAATATC AAGATGGCTG TATTGTTTGG 
CACCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTCC AACACAGTGA ATATATTACC 
CATGAAATAA CAGTGTTGAT TATTTATAAC CTTTTGACAA TAGTGGAGAT GCTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGTGTCTGA TTCTCAGAAA CAGAATCCAC 
GATCCCTATG ATTGTAACAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGTGCAGCT GGATGTTTTT ACCCTCAGCG GCTTTCGGAC TATACAGATC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCCT ACCAGGTAGA CCTGTTTGGT 
ATAGCAGATT TAGCGCATTT ACTATTGTTC AAGGAACACC TACAGGTCTT CTGGGATGGG 
TCCTTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTGTGC GGATTCTGAA TGCCAATGAT GAGGCCACAG TGTCTGTTCT TGGGGAGCTT 
GCAGCAGAAA TGAATGGGGT TTTTGACACT ACATTCCAAA GTCACCTGAA CAAAGCCTTA 
TGGAAGGTAG GGAAGTTAAC TAGTCCTGGG GCTTTGCTCT TTCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed