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IRS1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Summary

Gene Symbol IRS1
Entrez Gene ID 459985
Full Name insulin receptor substrate 1
General protein information
Preferred Names

insulin receptor substrate 1

Names

insulin receptor substrate 1
Gene Type protein-coding
Organism Pan troglodytes(chimpanzee)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_003309496.3 XP_003309544.1 insulin receptor substrate 1
XM_003309496.4 XP_003309544.1 insulin receptor substrate 1
XM_009444442.2 XP_009442717.1 insulin receptor substrate 1
XM_009444442.3 XP_009442717.1 insulin receptor substrate 1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Irs1 NP_037101.1
Gallus gallus (chicken) IRS1 XP_004951158.1
Homo sapiens (human) IRS1 NP_005535.1
Pan troglodytes (chimpanzee) IRS1 XP_001134895.1
Xenopus tropicalis (tropical clawed frog) LOC100495320 XP_004914519.1
Canis lupus familiaris (dog) IRS1 XP_543274.3
Danio rerio (zebrafish) irs1 XP_005157831.1
Bos taurus (cattle) IRS1 XP_003585821.1
Mus musculus (house mouse) Irs1 NP_034700.2

Gene Ontology

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc06009 XM_009444442.3
Latest version!		 Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $713.30
$1019.00
OPc06009 XM_003309496.4
Latest version!		 Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $713.30
$1019.00
OPc80762 XM_003309496.3
Latest version!		 Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00
OPc80762 XM_009444442.2
Latest version!		 Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OPc06009
Clone ID Related Accession (Same CDS sequence) XM_003309496.4 , XM_009444442.3
Accession Version XM_003309496.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-19
Organism Pan troglodytes(chimpanzee)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003309496.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCACCCC CACCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003309544.1
CDS1026..4751
Translation

Target ORF information:

RefSeq Version XM_003309496.4
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003309496.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCACCCC CACCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc06009
Clone ID Related Accession (Same CDS sequence) XM_003309496.4 , XM_009444442.3
Accession Version XM_009444442.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-03-19
Organism Pan troglodytes(chimpanzee)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCACCCC CACCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009442717.1
CDS43..3768
Translation

Target ORF information:

RefSeq Version XM_009444442.3
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009444442.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCACCCC CACCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc80762
Clone ID Related Accession (Same CDS sequence) XM_003309496.3 , XM_009444442.2
Accession Version XM_003309496.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-01
Organism Pan troglodytes(chimpanzee)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003309496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCCCCCC CCCCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_003309544.1
CDS1134..4859
Translation

Target ORF information:

RefSeq Version XM_003309496.3
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_003309496.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCCCCCC CCCCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OPc80762
Clone ID Related Accession (Same CDS sequence) XM_003309496.3 , XM_009444442.2
Accession Version XM_009444442.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-01
Organism Pan troglodytes(chimpanzee)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCCCCCC CCCCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009442717.1
CDS1135..4860
Translation

Target ORF information:

RefSeq Version XM_009444442.2
Organism Pan troglodytes(chimpanzee)
Definition Pan troglodytes insulin receptor substrate 1 (IRS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009444442.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGAGCC CTCCGGAGAG CGATGGCTTC TCGGACGTGC GCAAGGTGGG CTACCTGCGC 
AAACCCAAGA GCATGCACAA ACGCTTCTTC GTACTGCGCG CGGCCAGCGA GGCTGGGGGC 
CCGGCGCGCC TCGAGTACTA CGAGAACGAG AAGAAGTGGC GGCACAAGTC GAGCGCCCCC 
AAACGCTCGA TCCCCCTTGA GAGCTGCTTC AACATCAACA AGCGGGCTGA CTCCAAGAAC 
AAGCACCTGG TGGCTCTCTA CACCCGGGAC GAGCACTTTG CCATCGCGGC GGACAGCGAG 
GCCGAGCAAG ACAGCTGGTA CCAGGCTCTC CTACAGCTGC ACAACCGTGC TAAGGGCCAC 
CACGACGGAG CTGCGGCCCT CGGGGCGGGA GGTGGTGGGG GCAGCTGCAG CGGCAGCTCC 
GGCCTTGGTG AGGCTGGGGA GGACTTGAGC TACGGTGACG TGCCCCCAGG ACCCGCATTC 
AAAGAGGTCT GGCAAGTGAT CCTGAAGCCC AAGGGCCTGG GTCAGACAAA GAACCTGATT 
GGTATCTACC GCCTTTGCCT GACCAGCAAG ACCATCAGCT TCGTGAAGCT GAACTCGGAG 
GCAGCGGCCG TGGTGCTGCA GCTGATGAAC ATCAGGCGCT GTGGCCACTC GGAAAACTTC 
TTCTTCATCG AGGTGGGCCG TTCTGCCGTG ACGGGGCCCG GGGAGTTCTG GATGCAGGTG 
GATGACTCTG TGGTGGCCCA GAACATGCAC GAGACCATCC TGGAGGCCAT GCGGGCCATG 
AGTGATGAGT TCCGCCCTCG CAGCAAGAGC CAGTCCTCGT CCAACTGCTC TAACCCCATC 
AGCGTCCCCC TGCGCCGGCA CCATCTCAAC AATCCCCCGC CCAGCCAGGT GGGGCTGACC 
CGCCGATCGC GCACTGAGAG CATCACCGCC ACCTCCCCGG CCAGCATGGT GGGCGGGAAG 
CCAGGCTCCT TCCGTGTCCG CGCCTCCAGT GACGGCGAAG GCACCATGTC CCGCCCAGCC 
TCGGTGGACG GCAGCCCTGT GAGTCCCAGC ACCAACAGAA CCCACGCCCA CCGGCATCGG 
GGCAGCGCCC GGCTGCACCC CCCGCTCAAC CACAGCCGCT CCATCCCCAT GCCGGCTTCC 
CGCTGCTCGC CTTCGGCCAC CAGCCCGGTC AGTCTGTCGT CCAGTAGCAC CAGTGGCCAT 
GGCTCCACCT CGGATTGTCT CTTCCCACGG CGATCTAGTG CTTCGGTGTC TGGTTCCCCC 
AGCGATGGCG GTTTCATCTC CTCGGATGAG TATGGCTCCA GTCCCTGCGA TTTCCGGAGT 
TCCTTCCGCA GTGTCACTCC GGATTCCCTG GGCCACACCC CACCAGCCCG CGGTGAGGAG 
GAGCTAAGCA ACTATATCTG CATGGGTGGT AAGGGGCCCT CCACCCTGAC CGCCCCCAAC 
GGTCACTACA TTTTGTCTCG GGGTGGCAAT GGCCACCGCT GCACCCCAGG AACAGGCTTG 
GGCACGAGTC CAGCCTTGGC TGGGGATGAA GCAGCCAGTG CTGCAGATCT GGATAATCGG 
TTCCGAAAGA GAACTCACTC GGCAGGCACA TCCCCTACCA TTAACCACCA GAAGACCCCG 
TCCCAGTCCT CAGTGGCTTC CATTGAGGAG TACACAGAGA TGATGCCTGC CTACCCACCA 
GGAGGTGGCA GTGGAGGCCG ACTGCCGGGA CACAGGCACT CCGCCTTCGT GCCCACCCAC 
TCCTACCCAG AGGAGGGTCT GGAAATGCAC CCCTTGGAGC GTCGGGGGGG GCACCACCGC 
CCAGACAGCT CCACCCTCCA CACGGATGAT GGCTACATGC CCATGTCCCC AGGGGTGGCC 
CCAGTGCCCA GCGGCCGAAA GGGCAGTGGA GACTATATGC CCATGAGCCC CAAGAGCGTA 
TCTGCCCCAC AGCAGATCAT CAATCCCATC AGACGCCATC CCCAGAGAGT GGACCCCAAT 
GGCTACATGA TGATGTCCCC CAGCGGCGGC TGCTCTCCTG ACATTGGAGG TGGCCCCAGC 
AGCAGCAGCA GCAGCAATGC CGTCCCTTCC GGGACCAGCT ATGGAAAGCT GTGGACAAAC 
GGGGTAGGGG GCCACCACTC TCATGTCTTG CCTCACCCCA AACCCCCAGT GGAGAGCAGC 
GGTGGTAAGC TCTTACCTTG CACAGGTGAC TACATGAACA TGTCACCAGT GGGGGACTCC 
AACACCAGCA GCCCCTCCGA CTGCTACTAC GGCCCTGAGG ACCCCCAGCA CAAGCCAGTC 
CTCTCCTACT ACTCATTGCC AAGATCCTTT AAGCACACCC AGCGCCCCGG GGAGCCGGAG 
GAGGGTGCCC GGCATCAGCA CCTCCGCCTT TCCACTAGCT CTGGTCGCCT TCTCTATGCT 
GCAACAGCGG ATGATTCTTC CTCTTCCACC AGCAGCGACA GCCTGGGTGG GGGATACTGC 
GGGGCTAGGC TGGAGCCCAG CCTTCCACAT CCCCACCATC AGGTTCTGCA GCCCCATCTG 
CCTCGAAAGG TGGACACAGC TGCTCAGACC AATAGCCGCC TGGCCCGGCC CACGAGGCTG 
TCCCTGGGGG ATCCCAAGGC CAGCACCTTA CCTCGGGCCC GAGAGCAGCA GCAGCAGCAG 
CCCTTGCTGC ACCCTCCAGA GCCCAAGAGC CCGGGGGAAT ATGTCAATAT TGAATTTGGG 
AGTGATCAGC CTGGCTACTT GTCTGGCCCG GTGGCTTCCC ACAGCTCACC TTCTGTCAGG 
TGTCCATCCC AGCTCCAGCC AGCTCCCAGA GAGGAAGAGA CTGGCACTGA GGAGTACATG 
AAGATGGACC TGGGGCCGGG CCGGAGGGCA GCTTGGCAGG AGAGCACTGG GGTCGAGATG 
GGCAGACTGG GCCCTGCACC TCCCGGGGCT GCTAGCATTT GCAGGCCTAC CCGGGCAGTG 
CCCAGCAGCC GGGGTGACTA CATGACCATG CAGATGAGTT GTCCCCGTCA GAGCTACGTG 
GACACCTCGC CAGCTGCCCC TGTAAGCTAT GCTGACATGC GGACAGGCAT TGCTGCAGAG 
GAGGTGAGCC TGCCCAGGGC CACCATGGTT GCTGCCTCCT CATCCTCAGC AGTCTCTGCT 
TCCCCGACTG GGCCTCAAGG GGCAGCAGAG CTGGCTGCCC ACTCGTCCCT GCTGGGGGGC 
CCACAAGGAC CTGGGGGCAT GAGCGCCTTC ACCCGGGTGA ACCTCAGTCC TAACCGCAAC 
CAGAGTGCCA AAGTGATCCG TGCAGACCCA CAAGGGTGCC GGCGGAGGCA TAGCTCCGAG 
ACTTTCTCCT CAACACCCAG TGCCACCCGG GTGGGCAACA CAGTGCCCTT TGGAGCGGGG 
GCAACAGTAG GGGGCGGTGG CGGTAGCAGC AGCAGCAGCA GCGAGGATGT GAAACGCCAC 
AGCTCTGCTT CCTTTGAGAA TGTGTGGCTG AGGCCTGGGG AGCTTGGGGG AGCCCCCAAG 
GAGCCAGCCC AACTGTGTGG GGCTGCTGGG GGTTTGGAGA ATGGTCTTAA CTACATAGAC 
CTGGATTTGG TCAAGGACTT CAAACAGTGC CCTCAGGAGT GCACCCCTGA ACCGCAGCCT 
CCCCCCCCCC CCCCCCCTCA TCAACCCCTG GGCAGCGGTG AGAGCAGCTC CACCCGCCGC 
TCAAGTGAGG ATTTAAGCGC CTATGCCAGC ATCAGTTTCC AGAAGCAGCC AGAGGACCGT 
CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed