WNK1 gene cDNA ORF clone, Pongo abelii(Sumatran orangutan)

mRNA	Protein	Name
XM_009247289.1	XP_009245564.1	serine/threonine-protein kinase WNK1 isoform X3
XM_009247292.1	XP_009245567.1	serine/threonine-protein kinase WNK1 isoform X6
XM_009247289.2	XP_009245564.1	serine/threonine-protein kinase WNK1 isoform X5
XM_009247292.2	XP_009245567.1	serine/threonine-protein kinase WNK1 isoform X8
XM_009247294.2	XP_009245569.1	serine/threonine-protein kinase WNK1 isoform X10
XM_024256870.1	XP_024112638.1	serine/threonine-protein kinase WNK1 isoform X11
XM_009247290.1	XP_009245565.1	serine/threonine-protein kinase WNK1 isoform X4
XM_009247287.2	XP_009245562.1	serine/threonine-protein kinase WNK1 isoform X1
XM_009247293.2	XP_009245568.1	serine/threonine-protein kinase WNK1 isoform X9
XM_009247295.1	XP_009245570.1	serine/threonine-protein kinase WNK1 isoform X12
XM_009247287.1	XP_009245562.1	serine/threonine-protein kinase WNK1 isoform X1
XM_009247293.1	XP_009245568.1	serine/threonine-protein kinase WNK1 isoform X7
XM_009247294.1	XP_009245569.1	serine/threonine-protein kinase WNK1 isoform X8
XM_024256869.1	XP_024112637.1	serine/threonine-protein kinase WNK1 isoform X4
XM_009247291.1	XP_009245566.1	serine/threonine-protein kinase WNK1 isoform X5
XM_024256868.1	XP_024112636.1	serine/threonine-protein kinase WNK1 isoform X2
XM_009247288.2	XP_009245563.1	serine/threonine-protein kinase WNK1 isoform X3
XM_009247290.2	XP_009245565.1	serine/threonine-protein kinase WNK1 isoform X6
XM_009247288.1	XP_009245563.1	serine/threonine-protein kinase WNK1 isoform X2
XM_009247291.2	XP_009245566.1	serine/threonine-protein kinase WNK1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa167526	XM_024256868.1 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa167527	XM_024256869.1 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa167528	XM_024256870.1 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa26704	XM_009247287.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSa26705	XM_009247288.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSa26706	XM_009247289.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26707	XM_009247290.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26708	XM_009247291.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26709	XM_009247292.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26710	XM_009247293.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26711	XM_009247294.2 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26712	XM_009247295.1 Latest version!	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OSa26706	XM_009247289.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26709	XM_009247292.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26704	XM_009247287.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSa26710	XM_009247293.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26711	XM_009247294.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26707	XM_009247290.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OSa26705	XM_009247288.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OSa26708	XM_009247291.1	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

WNK1 ( XM_024256868.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024112636.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa167526
Clone ID Related Accession (Same CDS sequence)	XM_024256868.1
Accession Version	XM_024256868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC 
CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT 
GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTTCCT TGCTCCCTCA GTACCCTGTC 
TCTCAAATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT 
CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA 
ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG 
CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC 
ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT 
CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCC ACGTGGCTTC TGTTTGCACC CACCCTACAG TCCTGTCCCC TCCCATGCCG 
ACAGAAGCAC TGGCTGCACC TGGGCTCTTT CCCACGGTGG CGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTGTGGG TGCTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGTG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTAC AGAGCCAGTT CCAGTAGCAC AGCCACAACC TACCCAGCCG 
ACCACTTTGG CTTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ATGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCATTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTCGTT CATTCTGCTG GAAGACGGTT CATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACATCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTACCCAAA TGACAGAAGG ACCCAATACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGTAC CAGTCACTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACGGGT GTGGCAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGT GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGAACATT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTCCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTCTGTT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAAT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTCTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTGCCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTTACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAC TGAAACCTGG AACTGCTCCC TCAAAGCCAC CTCTAACCAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT TATCACAGTG ACTTCTGCGG TTGGTCCTGT GCCCGTGGTG 
GTTCCAACAG CAATTACAGA AGCAGGAGCA CAGCCTCAGA AGGATGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCAGTTG CAGCAGACGA TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACTTCAGAG TCCTCGGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGG ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAAGAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAAAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCATCT TCTGACCTGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCAGG TAGTCCACAC TCCCCCCATC AACTGAGCTC AAAGAGCCTT 
CCTATCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGT 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTA 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGAAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GGACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAGTCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGTA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAGA GGGCACACAA ATTATGAGGG CCCTGGAATG 
GCAAGGAAGT TTTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCAAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAGTGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024112636.1
CDS	635..7780
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_024256868.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024256868.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC 
CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT 
GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTTCCT TGCTCCCTCA GTACCCTGTC 
TCTCAAATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT 
CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA 
ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG 
CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC 
ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT 
CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT 
CCCTCTTCCC ACGTGGCTTC TGTTTGCACC CACCCTACAG TCCTGTCCCC TCCCATGCCG 
ACAGAAGCAC TGGCTGCACC TGGGCTCTTT CCCACGGTGG CGCAGCCTTA TGTGGAATCA 
AATCTTTTAG TTCCTGTGGG TGCTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGTG 
AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA 
GTCTCTCAGG TTGCTCCTAC AGAGCCAGTT CCAGTAGCAC AGCCACAACC TACCCAGCCG 
ACCACTTTGG CTTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT 
GGCAATGAGA ATGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT 
TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT 
AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCATTTGT GGATCAAGTG 
CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG 
AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTCGTT CATTCTGCTG GAAGACGGTT CATAGTGAGT 
CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA 
GTTGCTGCCT CTACATCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT 
AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTACCCAAA TGACAGAAGG ACCCAATACA 
GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGTAC CAGTCACTGC AACAAGCAGC 
CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG 
GGGATTGCTG GAGTTGCCAC CAGCACGGGT GTGGCAACTT CAGGTGGTCT CCCCATACCA 
CCTGTGTCTG AATCACCAGT ACTTTCCAGT GTAGTTTCAA GTATCACAAT ACCTGCAGTT 
GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGAACATT 
GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG 
GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC 
AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA 
GCTTCACAGC TGTCCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC 
GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTCTGTT 
TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT 
CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAAT AGATTCTGAT 
ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT 
CTCTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA 
CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA 
CTCCTGACTT CTGCCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT 
GCTTTGCCAG TTACACCAGT GGTTACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT 
ACATCAGGAC TGAAACCTGG AACTGCTCCC TCAAAGCCAC CTCTAACCAA GGCTCCGGTG 
CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT 
CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA 
AGAACACTTA GTCCAGAGAT TATCACAGTG ACTTCTGCGG TTGGTCCTGT GCCCGTGGTG 
GTTCCAACAG CAATTACAGA AGCAGGAGCA CAGCCTCAGA AGGATGTTTC TCAAGTCAAA 
GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG 
GTTTCAGTTG CAGCAGACGA TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG 
TCTGTTCATT TTGAATCCAG CACTTCAGAG TCCTCGGTGC TATCAAGTAG TAGTCCAGAG 
AGTACCTTGG TGAAACCAGA GCCGAATGGG ATAACCATCC CTGGTATCTC TTCAGATGTG 
CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC 
AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAAGAAAG TGGGTCGTTT CTCTGTATCA 
AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT 
ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAAAA GCCTGAACTG 
TCAGAGCCTT CACATCTAAA TGGGCCATCT TCTGACCTGG AGGCCGCTTT TTTAAGTAGG 
GATGTGGATG ATGGTTCAGG TAGTCCACAC TCCCCCCATC AACTGAGCTC AAAGAGCCTT 
CCTATCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGT 
GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT 
AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTA 
TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA 
GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGAAGCAAAT CTAGTCGAAG CAGTTCCTTG 
GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG 
TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GGACCAGCAG CACAAACACT 
GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAGTCTCAGC CTCCTGCCAT GACGTCCAGC 
AGGAAGGGTA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAGA GGGCACACAA ATTATGAGGG CCCTGGAATG 
GCAAGGAAGT TTTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCAAA CCTGGGTGGC 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAGTGGAG TGGGACGGGT 
GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_024256869.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024112637.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OSa167527
Clone ID Related Accession (Same CDS sequence)	XM_024256869.1
Accession Version	XM_024256869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA ACCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT 
GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG 
CCGCTCCCTA CTTCCTTGCT CCCTCAGTAC CCTGTCTCTC AAATTCCCAT ATCAACTCCT 
CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT 
ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA 
ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG 
GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA 
CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCCACGT GGCTTCTGTT 
TGCACCCACC CTACAGTCCT GTCCCCTCCC ATGCCGACAG AAGCACTGGC TGCACCTGGG 
CTCTTTCCCA CGGTGGCGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TGTGGGTGCT 
GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGTGAGTT TAGCACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTACAGAG 
CCAGTTCCAG TAGCACAGCC ACAACCTACC CAGCCGACCA CTTTGGCTTC CTCTATAGAC 
AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAATGT CCCATCTTCC 
AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC 
TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA 
GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT 
GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT 
CTAGCAATAG AGAGAGAGTC ATTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT 
GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ACCAGGGATT GGAGAGTCTA 
CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA 
CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA 
GTCGTTCATT CTGCTGGAAG ACGGTTCATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA 
GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC ATCTCAGAGC 
CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA 
CTTAGACGTA CCCAAATGAC AGAAGGACCC AATACAGCAC CTCCAAACTT TAGTCATACA 
GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA 
GCAGCAGCCA CAGTACCAGT CACTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA 
GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC 
ACGGGTGTGG CAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT 
TCCAGTGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG 
TCACTTCAAG TCCCCACATC CACATCTGAG AACATTGTTT CTAGTACAGC ACTGTATCCT 
TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT 
AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA 
ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTC CATTCAGCTT 
AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA 
GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC 
TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TCTGTTTCTC AGCCTGGTGG GCTGCATCCT 
TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT 
TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC 
CAGCCCCATA CTCATTGTCC TGAAATAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTCT TCAGTGAACA CAGCTCATCT 
GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA 
CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTGC CACAAGTACT 
TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTT 
ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGACTGAA ACCTGGAACT 
GCTCCCTCAA AGCCACCTCT AACCAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC 
CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATTATC 
ACAGTGACTT CTGCGGTTGG TCCTGTGCCC GTGGTGGTTC CAACAGCAAT TACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT 
TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CAGTTGCAGC AGACGATGCC 
CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CGGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG 
AATGGGATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT 
GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA AGAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC 
ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT 
CCTGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG 
CCATCTTCTG ACCTGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAACT GAGCTCAAAG AGCCTTCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATCGAAGAT 
GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTATATA CCAAACTGGG CAAGGTGCCC 
CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA 
AGCAAAGGAA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT 
TCTGCTCCAG GCCAAGGGAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA 
GCCGCCCAGT CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGTACATT CACAGATGAC 
TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAGAGGGC ACACAAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTTTC TGCACCTGGG 
CAACTGTGCA TCTCCATGAC CTCAAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA 
GCTACCCCAT TTGGCGCTCA GTGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC 
CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024112637.1
CDS	635..7696
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_024256869.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024256869.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA ACCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT 
GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG 
CCGCTCCCTA CTTCCTTGCT CCCTCAGTAC CCTGTCTCTC AAATTCCCAT ATCAACTCCT 
CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT 
ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA 
ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG 
GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA 
CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCCACGT GGCTTCTGTT 
TGCACCCACC CTACAGTCCT GTCCCCTCCC ATGCCGACAG AAGCACTGGC TGCACCTGGG 
CTCTTTCCCA CGGTGGCGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TGTGGGTGCT 
GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGTGAGTT TAGCACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTACAGAG 
CCAGTTCCAG TAGCACAGCC ACAACCTACC CAGCCGACCA CTTTGGCTTC CTCTATAGAC 
AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAATGT CCCATCTTCC 
AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC 
TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA 
GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT 
GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT 
CTAGCAATAG AGAGAGAGTC ATTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT 
GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ACCAGGGATT GGAGAGTCTA 
CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA 
CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA 
GTCGTTCATT CTGCTGGAAG ACGGTTCATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA 
GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC ATCTCAGAGC 
CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA 
CTTAGACGTA CCCAAATGAC AGAAGGACCC AATACAGCAC CTCCAAACTT TAGTCATACA 
GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA 
GCAGCAGCCA CAGTACCAGT CACTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA 
GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC 
ACGGGTGTGG CAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT 
TCCAGTGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG 
TCACTTCAAG TCCCCACATC CACATCTGAG AACATTGTTT CTAGTACAGC ACTGTATCCT 
TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT 
AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA 
ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTC CATTCAGCTT 
AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA 
GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC 
TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TCTGTTTCTC AGCCTGGTGG GCTGCATCCT 
TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT 
TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC 
CAGCCCCATA CTCATTGTCC TGAAATAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTCT TCAGTGAACA CAGCTCATCT 
GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA 
CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTGC CACAAGTACT 
TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTT 
ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGACTGAA ACCTGGAACT 
GCTCCCTCAA AGCCACCTCT AACCAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC 
CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATTATC 
ACAGTGACTT CTGCGGTTGG TCCTGTGCCC GTGGTGGTTC CAACAGCAAT TACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT 
TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CAGTTGCAGC AGACGATGCC 
CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CGGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG 
AATGGGATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT 
GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA AGAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC 
ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT 
CCTGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG 
CCATCTTCTG ACCTGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAACT GAGCTCAAAG AGCCTTCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATCGAAGAT 
GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTATATA CCAAACTGGG CAAGGTGCCC 
CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA 
AGCAAAGGAA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT 
TCTGCTCCAG GCCAAGGGAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA 
GCCGCCCAGT CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGTACATT CACAGATGAC 
TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAGAGGGC ACACAAATTA TGAGGGCCCT GGAATGGCAA GGAAGTTTTC TGCACCTGGG 
CAACTGTGCA TCTCCATGAC CTCAAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA 
GCTACCCCAT TTGGCGCTCA GTGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC 
CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_024256870.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024112638.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OSa167528
Clone ID Related Accession (Same CDS sequence)	XM_024256870.1
Accession Version	XM_024256870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA ACCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTACAG AGCCAGTTCC AGTAGCACAG 
CCACAACCTA CCCAGCCGAC CACTTTGGCT TCCTCTATAG ACAGTGCACA TTCAGATGTT 
GCTTCAGGTA TGAGTGATGG CAATGAGAAT GTCCCATCTT CCAGTGGAAG GCATGAAGGA 
AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT 
TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT 
CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC 
CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG 
TCATTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT 
GTCAGTGTGG AACCAGAGGG TGACCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT 
GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC 
ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTCGTTCA TTCTGCTGGA 
AGACGGTTCA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC 
AGTGAAATAA CAGATACAGT TGCTGCCTCT ACATCTCAGA GCCCTGGAAT GAACTTGTCT 
CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TACCCAAATG 
ACAGAAGGAC CCAATACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA 
GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGTACCA 
GTCACTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT 
ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACGGGTGT GGCAACTTCA 
GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGTGT AGTTTCAAGT 
ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA 
TCCACATCTG AGAACATTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA 
ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA 
TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC 
ACTTCATTCC CAAGCACAGC TTCACAGCTG TCCATTCAGC TTAGCAGCAG TACTTCTACT 
CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC 
AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA 
GGAGTGTCTA GTTCTGTTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG 
ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC 
CAAGTACCTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTACAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT 
CCTGAAATAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAAGAAAAGC TGCGGTCTCT CTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT 
GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT 
GCTGTTGCAC CAAGCAAACT CCTGACTTCT GCCACAAGTA CTTGCTTACC ACCAACCAAT 
TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TTACACCTGG GCAAGTTTCT 
ACCCCAGTCA GCACTACTAC ATCAGGACTG AAACCTGGAA CTGCTCCCTC AAAGCCACCT 
CTAACCAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT 
CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATTA TCACAGTGAC TTCTGCGGTT 
GGTCCTGTGC CCGTGGTGGT TCCAACAGCA ATTACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT 
AAGATGGGAC GATTTCAGGT TTCAGTTGCA GCAGACGATG CCCAGAAAGA GGGTAAAAAT 
AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCGGTGCTA 
TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGGAT AACCATCCCT 
GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA 
GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAAGAAAGTG 
GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA 
GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCATCTTC TGACCTGGAG 
GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAA 
CTGAGCTCAA AGAGCCTTCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG 
CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTATA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC 
CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG AAGCAAATCT 
AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG 
TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT 
TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGCCAAGGG 
ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA GTCTCAGCCT 
CCTGCCATGA CGTCCAGCAG GAAGGGTACA TTCACAGATG ACTTGCACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAGAGG GCACACAAAT 
TATGAGGGCC CTGGAATGGC AAGGAAGTTT TCTGCACCTG GGCAACTGTG CATCTCCATG 
ACCTCAAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT 
CAGTGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA 
ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024112638.1
CDS	635..6958
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_024256870.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024256870.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC 
CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT 
ACTGCACAGC CAGTGAGTCA ACCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA 
AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTACAG AGCCAGTTCC AGTAGCACAG 
CCACAACCTA CCCAGCCGAC CACTTTGGCT TCCTCTATAG ACAGTGCACA TTCAGATGTT 
GCTTCAGGTA TGAGTGATGG CAATGAGAAT GTCCCATCTT CCAGTGGAAG GCATGAAGGA 
AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT 
TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT 
CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC 
CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG 
TCATTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT 
GTCAGTGTGG AACCAGAGGG TGACCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT 
GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC 
ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTCGTTCA TTCTGCTGGA 
AGACGGTTCA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC 
AGTGAAATAA CAGATACAGT TGCTGCCTCT ACATCTCAGA GCCCTGGAAT GAACTTGTCT 
CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TACCCAAATG 
ACAGAAGGAC CCAATACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA 
GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGTACCA 
GTCACTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT 
ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACGGGTGT GGCAACTTCA 
GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGTGT AGTTTCAAGT 
ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA 
TCCACATCTG AGAACATTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA 
ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA 
TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC 
ACTTCATTCC CAAGCACAGC TTCACAGCTG TCCATTCAGC TTAGCAGCAG TACTTCTACT 
CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC 
AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA 
GGAGTGTCTA GTTCTGTTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG 
ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC 
CAAGTACCTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTACAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT 
CCTGAAATAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAAGAAAAGC TGCGGTCTCT CTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT 
GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT 
GCTGTTGCAC CAAGCAAACT CCTGACTTCT GCCACAAGTA CTTGCTTACC ACCAACCAAT 
TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TTACACCTGG GCAAGTTTCT 
ACCCCAGTCA GCACTACTAC ATCAGGACTG AAACCTGGAA CTGCTCCCTC AAAGCCACCT 
CTAACCAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT 
CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATTA TCACAGTGAC TTCTGCGGTT 
GGTCCTGTGC CCGTGGTGGT TCCAACAGCA ATTACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT 
AAGATGGGAC GATTTCAGGT TTCAGTTGCA GCAGACGATG CCCAGAAAGA GGGTAAAAAT 
AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCGGTGCTA 
TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGGAT AACCATCCCT 
GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA 
GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAAGAAAGTG 
GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA 
GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCATCTTC TGACCTGGAG 
GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAA 
CTGAGCTCAA AGAGCCTTCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG 
CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTATA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC 
CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG AAGCAAATCT 
AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG 
TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT 
TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGCCAAGGG 
ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA GTCTCAGCCT 
CCTGCCATGA CGTCCAGCAG GAAGGGTACA TTCACAGATG ACTTGCACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAGAGG GCACACAAAT 
TATGAGGGCC CTGGAATGGC AAGGAAGTTT TCTGCACCTG GGCAACTGTG CATCTCCATG 
ACCTCAAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT 
CAGTGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA 
ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247287.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245562.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OSa26704
Clone ID Related Accession (Same CDS sequence)	XM_009247287.2 , XM_009247287.1
Accession Version	XM_009247287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245562.1
CDS	838..7986
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247287.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247287.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247287.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245562.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OSa26704
Clone ID Related Accession (Same CDS sequence)	XM_009247287.2 , XM_009247287.1
Accession Version	XM_009247287.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245562.1
CDS	838..7986
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247287.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247287.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247288.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245563.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OSa26705
Clone ID Related Accession (Same CDS sequence)	XM_009247288.2 , XM_009247288.1
Accession Version	XM_009247288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTTCCTT GCTCCCTCAG TACCCTGTCT CTCAAATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCCA CGTGGCTTCT 
GTTTGCACCC ACCCTACAGT CCTGTCCCCT CCCATGCCGA CAGAAGCACT GGCTGCACCT 
GGGCTCTTTC CCACGGTGGC GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTGTGGGT 
GCTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGTGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTACA 
GAGCCAGTTC CAGTAGCACA GCCACAACCT ACCCAGCCGA CCACTTTGGC TTCCTCTATA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA TGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCATTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGACCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTCGTTC ATTCTGCTGG AAGACGGTTC ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACATCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTACCCAAAT GACAGAAGGA CCCAATACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGTACC AGTCACTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACGGGTG TGGCAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGTG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGAACATTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTCCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTCTGTTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAATA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TCTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TGCCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTTACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGACT GAAACCTGGA 
ACTGCTCCCT CAAAGCCACC TCTAACCAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATT 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG CCCGTGGTGG TTCCAACAGC AATTACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCAGTTGC AGCAGACGAT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCGGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGGA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAAGAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCATCTT CTGACCTGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA ACTGAGCTCA AAGAGCCTTC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTAT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GAAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGCCAAGG GACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AGTCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGTAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAGAG GGCACACAAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT TTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAGTGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245563.1
CDS	635..7699
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247288.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247288.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTTCCTT GCTCCCTCAG TACCCTGTCT CTCAAATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCCA CGTGGCTTCT 
GTTTGCACCC ACCCTACAGT CCTGTCCCCT CCCATGCCGA CAGAAGCACT GGCTGCACCT 
GGGCTCTTTC CCACGGTGGC GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTGTGGGT 
GCTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGTGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTACA 
GAGCCAGTTC CAGTAGCACA GCCACAACCT ACCCAGCCGA CCACTTTGGC TTCCTCTATA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA TGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCATTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGACCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTCGTTC ATTCTGCTGG AAGACGGTTC ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACATCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTACCCAAAT GACAGAAGGA CCCAATACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGTACC AGTCACTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACGGGTG TGGCAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGTG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGAACATTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTCCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTCTGTTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAATA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TCTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TGCCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTTACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGACT GAAACCTGGA 
ACTGCTCCCT CAAAGCCACC TCTAACCAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATT 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG CCCGTGGTGG TTCCAACAGC AATTACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCAGTTGC AGCAGACGAT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCGGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGGA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAAGAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCATCTT CTGACCTGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA ACTGAGCTCA AAGAGCCTTC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTAT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GAAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGCCAAGG GACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AGTCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGTAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAGAG GGCACACAAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT TTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAGTGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247288.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245563.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OSa26705
Clone ID Related Accession (Same CDS sequence)	XM_009247288.2 , XM_009247288.1
Accession Version	XM_009247288.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTTCCTT GCTCCCTCAG TACCCTGTCT CTCAAATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCCA CGTGGCTTCT 
GTTTGCACCC ACCCTACAGT CCTGTCCCCT CCCATGCCGA CAGAAGCACT GGCTGCACCT 
GGGCTCTTTC CCACGGTGGC GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTGTGGGT 
GCTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGTGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTACA 
GAGCCAGTTC CAGTAGCACA GCCACAACCT ACCCAGCCGA CCACTTTGGC TTCCTCTATA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA TGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCATTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGACCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTCGTTC ATTCTGCTGG AAGACGGTTC ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACATCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTACCCAAAT GACAGAAGGA CCCAATACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGTACC AGTCACTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACGGGTG TGGCAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGTG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGAACATTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTCCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTCTGTTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAATA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TCTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TGCCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTTACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGACT GAAACCTGGA 
ACTGCTCCCT CAAAGCCACC TCTAACCAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATT 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG CCCGTGGTGG TTCCAACAGC AATTACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCAGTTGC AGCAGACGAT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCGGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGGA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAAGAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCATCTT CTGACCTGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA ACTGAGCTCA AAGAGCCTTC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTAT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GAAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGCCAAGG GACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AGTCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGTAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAGAG GGCACACAAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT TTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAGTGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245563.1
CDS	838..7902
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247288.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247288.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA 
CAGCCGCTCC CTACTTCCTT GCTCCCTCAG TACCCTGTCT CTCAAATTCC CATATCAACT 
CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC 
ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA 
TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCCA CGTGGCTTCT 
GTTTGCACCC ACCCTACAGT CCTGTCCCCT CCCATGCCGA CAGAAGCACT GGCTGCACCT 
GGGCTCTTTC CCACGGTGGC GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTGTGGGT 
GCTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGTGA GTTTAGCACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTACA 
GAGCCAGTTC CAGTAGCACA GCCACAACCT ACCCAGCCGA CCACTTTGGC TTCCTCTATA 
GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA TGTCCCATCT 
TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT 
CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA 
GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA 
TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT 
ATTCTAGCAA TAGAGAGAGA GTCATTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGACCAGGG ATTGGAGAGT 
CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA 
CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT 
CAAGTCGTTC ATTCTGCTGG AAGACGGTTC ATAGTGAGTC CTGTGCCAGA AAGCCGATTA 
CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACATCTCAG 
AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT 
GAACTTAGAC GTACCCAAAT GACAGAAGGA CCCAATACAG CACCTCCAAA CTTTAGTCAT 
ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGCAG CCACAGTACC AGTCACTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA 
TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC 
AGCACGGGTG TGGCAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA 
CTTTCCAGTG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC 
CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGAACATTG TTTCTAGTAC AGCACTGTAT 
CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT 
CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA 
TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTCCATTCAG 
CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA 
CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT 
TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTCTGTTT CTCAGCCTGG TGGGCTGCAT 
CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT 
ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC 
AACCAGCCCC ATACTCATTG TCCTGAAATA GATTCTGATA CACAACCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TCTTCAGTGA ACACAGCTCA 
TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC 
ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TGCCACAAGT 
ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG 
GTTACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGACT GAAACCTGGA 
ACTGCTCCCT CAAAGCCACC TCTAACCAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT 
CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG 
TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATT 
ATCACAGTGA CTTCTGCGGT TGGTCCTGTG CCCGTGGTGG TTCCAACAGC AATTACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT 
AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCAGTTGC AGCAGACGAT 
GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCGGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG 
CCGAATGGGA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT 
ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAAGAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT 
GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT 
CTTCCTGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT 
GGGCCATCTT CTGACCTGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA ACTGAGCTCA AAGAGCCTTC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATCGAA 
GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTAT ATACCAAACT GGGCAAGGTG 
CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT 
AAAAGCAAAG GAAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC 
CTTTCTGCTC CAGGCCAAGG GACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC 
CAAGCCGCCC AGTCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGTAC ATTCACAGAT 
GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA 
GGAAGCAGAG GGCACACAAA TTATGAGGGC CCTGGAATGG CAAGGAAGTT TTCTGCACCT 
GGGCAACTGT GCATCTCCAT GACCTCAAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT 
CCAGCTACCC CATTTGGCGC TCAGTGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT 
GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247289.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245564.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OSa26706
Clone ID Related Accession (Same CDS sequence)	XM_009247289.1 , XM_009247289.2
Accession Version	XM_009247289.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGG ACCAGCAGCA CAAACACTGT TGGGGCAACA 
GTGAACAGCC AAGCCGCCCA GTCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGTACA 
TTCACAGATG ACTTGCACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAGAGG GCACACAAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTT 
TCTGCACCTG GGCAACTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGCTTTC CAGCTACCCC ATTTGGCGCT CAGTGGAGTG GGACGGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TGCAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245564.1
CDS	838..7791
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247289.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247289.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGG ACCAGCAGCA CAAACACTGT TGGGGCAACA 
GTGAACAGCC AAGCCGCCCA GTCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGTACA 
TTCACAGATG ACTTGCACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAGAGG GCACACAAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTT 
TCTGCACCTG GGCAACTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGCTTTC CAGCTACCCC ATTTGGCGCT CAGTGGAGTG GGACGGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TGCAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247289.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245564.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OSa26706
Clone ID Related Accession (Same CDS sequence)	XM_009247289.1 , XM_009247289.2
Accession Version	XM_009247289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGG ACCAGCAGCA CAAACACTGT TGGGGCAACA 
GTGAACAGCC AAGCCGCCCA GTCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGTACA 
TTCACAGATG ACTTGCACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAGAGG GCACACAAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTT 
TCTGCACCTG GGCAACTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGCTTTC CAGCTACCCC ATTTGGCGCT CAGTGGAGTG GGACGGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TGCAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245564.1
CDS	635..7588
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247289.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247289.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGG ACCAGCAGCA CAAACACTGT TGGGGCAACA 
GTGAACAGCC AAGCCGCCCA GTCTCAGCCT CCTGCCATGA CGTCCAGCAG GAAGGGTACA 
TTCACAGATG ACTTGCACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAGAGG GCACACAAAT TATGAGGGCC CTGGAATGGC AAGGAAGTTT 
TCTGCACCTG GGCAACTGTG CATCTCCATG ACCTCAAACC TGGGTGGCTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TATGGCTTTC CAGCTACCCC ATTTGGCGCT CAGTGGAGTG GGACGGGTGG CCCAGCACCA 
CAGCCACTTG GCCAGTTCCA ACCTGTGGGA ACTGCCTCCT TGCAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGCTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247290.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245565.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OSa26707
Clone ID Related Accession (Same CDS sequence)	XM_009247290.1 , XM_009247290.2
Accession Version	XM_009247290.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245565.1
CDS	838..7707
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247290.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247290.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247290.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245565.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OSa26707
Clone ID Related Accession (Same CDS sequence)	XM_009247290.1 , XM_009247290.2
Accession Version	XM_009247290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245565.1
CDS	635..7504
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTSLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247290.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247290.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT 
ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT 
GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTT CCTTGCTCCC TCAGTACCCT 
GTCTCTCAAA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC 
CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT 
ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG 
CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG 
TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT 
GTTCTGTACC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247291.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245566.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OSa26708
Clone ID Related Accession (Same CDS sequence)	XM_009247291.1 , XM_009247291.2
Accession Version	XM_009247291.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGGGCTTCC CACCTCGACT GCCACCACAG 
TACCCAGGAG ATTCAAATAT TGCTCCCTCT TCCCACGTGG CTTCTGTTTG CACCCACCCT 
ACAGTCCTGT CCCCTCCCAT GCCGACAGAA GCACTGGCTG CACCTGGGCT CTTTCCCACG 
GTGGCGCAGC CTTATGTGGA ATCAAATCTT TTAGTTCCTG TGGGTGCTGT AGGAGGACAG 
GTTCAAGTGT CCCAGCCAGG AGTGAGTTTA GCACAAGCCC CCACTACATC CTCCCAGCAA 
GCAGTTTTGG AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245566.1
CDS	838..7527
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247291.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247291.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGGGCTTCC CACCTCGACT GCCACCACAG 
TACCCAGGAG ATTCAAATAT TGCTCCCTCT TCCCACGTGG CTTCTGTTTG CACCCACCCT 
ACAGTCCTGT CCCCTCCCAT GCCGACAGAA GCACTGGCTG CACCTGGGCT CTTTCCCACG 
GTGGCGCAGC CTTATGTGGA ATCAAATCTT TTAGTTCCTG TGGGTGCTGT AGGAGGACAG 
GTTCAAGTGT CCCAGCCAGG AGTGAGTTTA GCACAAGCCC CCACTACATC CTCCCAGCAA 
GCAGTTTTGG AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247291.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245566.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OSa26708
Clone ID Related Accession (Same CDS sequence)	XM_009247291.1 , XM_009247291.2
Accession Version	XM_009247291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGGGCTTCC CACCTCGACT GCCACCACAG 
TACCCAGGAG ATTCAAATAT TGCTCCCTCT TCCCACGTGG CTTCTGTTTG CACCCACCCT 
ACAGTCCTGT CCCCTCCCAT GCCGACAGAA GCACTGGCTG CACCTGGGCT CTTTCCCACG 
GTGGCGCAGC CTTATGTGGA ATCAAATCTT TTAGTTCCTG TGGGTGCTGT AGGAGGACAG 
GTTCAAGTGT CCCAGCCAGG AGTGAGTTTA GCACAAGCCC CCACTACATC CTCCCAGCAA 
GCAGTTTTGG AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245566.1
CDS	635..7324
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247291.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247291.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGGGCTTCC CACCTCGACT GCCACCACAG 
TACCCAGGAG ATTCAAATAT TGCTCCCTCT TCCCACGTGG CTTCTGTTTG CACCCACCCT 
ACAGTCCTGT CCCCTCCCAT GCCGACAGAA GCACTGGCTG CACCTGGGCT CTTTCCCACG 
GTGGCGCAGC CTTATGTGGA ATCAAATCTT TTAGTTCCTG TGGGTGCTGT AGGAGGACAG 
GTTCAAGTGT CCCAGCCAGG AGTGAGTTTA GCACAAGCCC CCACTACATC CTCCCAGCAA 
GCAGTTTTGG AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTACAGAGCC AGTTCCAGTA 
GCACAGCCAC AACCTACCCA GCCGACCACT TTGGCTTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAATGTCC CATCTTCCAG TGGAAGGCAT 
GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA 
AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT 
GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG 
AGAGAGTCAT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTACA AGGAAAGGAT 
GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT CGTTCATTCT 
GCTGGAAGAC GGTTCATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT 
TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAT CTCAGAGCCC TGGAATGAAC 
TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTACC 
CAAATGACAG AAGGACCCAA TACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT 
CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA 
GTACCAGTCA CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT 
GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC GGGTGTGGCA 
ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGTGTAGTT 
TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC 
CCCACATCCA CATCTGAGAA CATTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT 
TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT 
GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT 
ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTCCA TTCAGCTTAG CAGCAGTACT 
TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT 
CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT 
GGAGCAGGAG TGTCTAGTTC TGTTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA 
TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA 
TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAACCTG TTGCCAGTGT GCCTGCTGTA 
CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT 
CATTGTCCTG AAATAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG 
ACTCTAGAAG AAAAGCTGCG GTCTCTCTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT 
GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA 
ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTGCCA CAAGTACTTG CTTACCACCA 
ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTTAC ACCTGGGCAA 
GTTTCTACCC CAGTCAGCAC TACTACATCA GGACTGAAAC CTGGAACTGC TCCCTCAAAG 
CCACCTCTAA CCAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA 
CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA 
GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATTATCAC AGTGACTTCT 
GCGGTTGGTC CTGTGCCCGT GGTGGTTCCA ACAGCAATTA CAGAAGCAGG AGCACAGCCT 
CAGAAGGATG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT 
GTTTTTAAGA TGGGACGATT TCAGGTTTCA GTTGCAGCAG ACGATGCCCA GAAAGAGGGT 
AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACTTC AGAGTCCTCG 
GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGGATAACC 
ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA 
AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAG 
AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA 
GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA 
CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC ATCTTCTGAC 
CTGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CAGGTAGTCC ACACTCCCCC 
CATCAACTGA GCTCAAAGAG CCTTCCTATC CAGAATCTAA GTCAAAGCCT TAGTAATTCA 
TTTAACTCCT CTTACATGAG TAGTGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG 
TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC 
CAGAAGCATG AAATTGAATC TTTATATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT 
ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGAAGC 
AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT 
GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC 
CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC 
CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAGTCT 
CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGTACATTCA CAGATGACTT GCACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAGAGGGCAC 
ACAAATTATG AGGGCCCTGG AATGGCAAGG AAGTTTTCTG CACCTGGGCA ACTGTGCATC 
TCCATGACCT CAAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT 
GGCGCTCAGT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT 
GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGCT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247292.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245567.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OSa26709
Clone ID Related Accession (Same CDS sequence)	XM_009247292.1 , XM_009247292.2
Accession Version	XM_009247292.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTACAGAGC CAGTTCCAGT AGCACAGCCA CAACCTACCC AGCCGACCAC TTTGGCTTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAATGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TCGTTCATTC TGCTGGAAGA CGGTTCATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
TCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTAC CCAAATGACA GAAGGACCCA ATACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGTACCAGTC ACTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CGGGTGTGGC AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGTGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA ACATTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTCC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT CTGTTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAACCT 
GTTGCCAGTG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAATAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTCTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTGCC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTTA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGACTGAAA 
CCTGGAACTG CTCCCTCAAA GCCACCTCTA ACCAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATTATCA CAGTGACTTC TGCGGTTGGT CCTGTGCCCG TGGTGGTTCC AACAGCAATT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC AGTTGCAGCA 
GACGATGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC GGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGGATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA GAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CATCTTCTGA CCTGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCAGGTAGTC CACACTCCCC CCATCAACTG AGCTCAAAGA GCCTTCCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTATATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGAAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG CCAAGGGACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAGTC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGTACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAGAGGGCA CACAAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTTTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAG TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245567.1
CDS	838..7248
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247292.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247292.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTACAGAGC CAGTTCCAGT AGCACAGCCA CAACCTACCC AGCCGACCAC TTTGGCTTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAATGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TCGTTCATTC TGCTGGAAGA CGGTTCATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
TCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTAC CCAAATGACA GAAGGACCCA ATACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGTACCAGTC ACTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CGGGTGTGGC AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGTGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA ACATTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTCC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT CTGTTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAACCT 
GTTGCCAGTG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAATAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTCTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTGCC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTTA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGACTGAAA 
CCTGGAACTG CTCCCTCAAA GCCACCTCTA ACCAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATTATCA CAGTGACTTC TGCGGTTGGT CCTGTGCCCG TGGTGGTTCC AACAGCAATT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC AGTTGCAGCA 
GACGATGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC GGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGGATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA GAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CATCTTCTGA CCTGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCAGGTAGTC CACACTCCCC CCATCAACTG AGCTCAAAGA GCCTTCCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTATATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGAAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG CCAAGGGACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAGTC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGTACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAGAGGGCA CACAAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTTTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAG TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247292.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245567.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OSa26709
Clone ID Related Accession (Same CDS sequence)	XM_009247292.1 , XM_009247292.2
Accession Version	XM_009247292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTACAGAGC CAGTTCCAGT AGCACAGCCA CAACCTACCC AGCCGACCAC TTTGGCTTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAATGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TCGTTCATTC TGCTGGAAGA CGGTTCATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
TCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTAC CCAAATGACA GAAGGACCCA ATACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGTACCAGTC ACTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CGGGTGTGGC AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGTGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA ACATTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTCC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT CTGTTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAACCT 
GTTGCCAGTG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAATAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTCTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTGCC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTTA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGACTGAAA 
CCTGGAACTG CTCCCTCAAA GCCACCTCTA ACCAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATTATCA CAGTGACTTC TGCGGTTGGT CCTGTGCCCG TGGTGGTTCC AACAGCAATT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC AGTTGCAGCA 
GACGATGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC GGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGGATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA GAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CATCTTCTGA CCTGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCAGGTAGTC CACACTCCCC CCATCAACTG AGCTCAAAGA GCCTTCCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTATATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGAAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG CCAAGGGACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAGTC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGTACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAGAGGGCA CACAAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTTTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAG TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245567.1
CDS	635..7045
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247292.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247292.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA 
CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAACC TCAAGCTCCA 
CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT 
CCTACAGAGC CAGTTCCAGT AGCACAGCCA CAACCTACCC AGCCGACCAC TTTGGCTTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAATGTC 
CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA 
AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA 
TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATAGA GAGAGAGTCA TTTGTGGATC AAGTGCGAGA AATTATTGAA 
AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG 
TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TCGTTCATTC TGCTGGAAGA CGGTTCATAG TGAGTCCTGT GCCAGAAAGC 
CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA 
TCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC 
TTTTCTGAAC TTAGACGTAC CCAAATGACA GAAGGACCCA ATACAGCACC TCCAAACTTT 
AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGCAGCCAC AGTACCAGTC ACTGCAACAA GCAGCCCTCC TAATGACATT 
TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT 
GCCACCAGCA CGGGTGTGGC AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA 
CCAGTACTTT CCAGTGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT 
ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA ACATTGTTTC TAGTACAGCA 
CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC 
CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA 
GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTCC 
ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA 
CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA 
CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT CTGTTTCTCA GCCTGGTGGG 
CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA 
GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAACCT 
GTTGCCAGTG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG 
CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAATAGATT CTGATACACA ACCCAAAGCT 
CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTCTT CAGTGAACAC 
AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC 
ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTGCC 
ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA 
CCAGTGGTTA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGACTGAAA 
CCTGGAACTG CTCCCTCAAA GCCACCTCTA ACCAAGGCTC CGGTGCTGCC AGTGGGTACT 
GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA 
ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA 
GAGATTATCA CAGTGACTTC TGCGGTTGGT CCTGTGCCCG TGGTGGTTCC AACAGCAATT 
ACAGAAGCAG GAGCACAGCC TCAGAAGGAT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA 
GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC AGTTGCAGCA 
GACGATGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA 
TCCAGCACTT CAGAGTCCTC GGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA 
CCAGAGCCGA ATGGGATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC 
AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT 
CAGGTGACAA CTACAGCAAA GAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG 
ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA 
GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAAAAGCCTG AACTGTCAGA GCCTTCACAT 
CTAAATGGGC CATCTTCTGA CCTGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT 
TCAGGTAGTC CACACTCCCC CCATCAACTG AGCTCAAAGA GCCTTCCTAT CCAGAATCTA 
AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGTGACAA TGAGTCAGAT 
ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG 
ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTATATAC CAAACTGGGC 
AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA 
CCCACTAAAA GCAAAGGAAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC 
CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC 
CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG 
CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA 
CCAAGCCTTT CTGCTCCAGG CCAAGGGACC AGCAGCACAA ACACTGTTGG GGCAACAGTG 
AACAGCCAAG CCGCCCAGTC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGTACATTC 
ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC 
AGGAGAGGAA GCAGAGGGCA CACAAATTAT GAGGGCCCTG GAATGGCAAG GAAGTTTTCT 
GCACCTGGGC AACTGTGCAT CTCCATGACC TCAAACCTGG GTGGCTCTGC CCCCATCTCT 
GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT 
GGCTTTCCAG CTACCCCATT TGGCGCTCAG TGGAGTGGGA CGGGTGGCCC AGCACCACAG 
CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT 
TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247293.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245568.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OSa26710
Clone ID Related Accession (Same CDS sequence)	XM_009247293.2 , XM_009247293.1
Accession Version	XM_009247293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
ACAGAGCCAG TTCCAGTAGC ACAGCCACAA CCTACCCAGC CGACCACTTT GGCTTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAATGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCATTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTCG TTCATTCTGC TGGAAGACGG TTCATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACATCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTACCCA AATGACAGAA GGACCCAATA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGT ACCAGTCACT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACGG GTGTGGCAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GTGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGAACA TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTCCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTCTG TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAACCTGTT 
GCCAGTGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA ATAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTCTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTGCCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTTACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG ACTGAAACCT 
GGAACTGCTC CCTCAAAGCC ACCTCTAACC AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATTATCACAG TGACTTCTGC GGTTGGTCCT GTGCCCGTGG TGGTTCCAAC AGCAATTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCAGT TGCAGCAGAC 
GATGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCGGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GGATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAAGAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCAT CTTCTGACCT GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA TCAACTGAGC TCAAAGAGCC TTCCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TATATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGAAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAGTCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG TACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA GAGGGCACAC AAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTTTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAGTGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245568.1
CDS	635..7042
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247293.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247293.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
ACAGAGCCAG TTCCAGTAGC ACAGCCACAA CCTACCCAGC CGACCACTTT GGCTTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAATGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCATTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTCG TTCATTCTGC TGGAAGACGG TTCATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACATCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTACCCA AATGACAGAA GGACCCAATA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGT ACCAGTCACT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACGG GTGTGGCAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GTGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGAACA TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTCCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTCTG TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAACCTGTT 
GCCAGTGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA ATAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTCTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTGCCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTTACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG ACTGAAACCT 
GGAACTGCTC CCTCAAAGCC ACCTCTAACC AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATTATCACAG TGACTTCTGC GGTTGGTCCT GTGCCCGTGG TGGTTCCAAC AGCAATTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCAGT TGCAGCAGAC 
GATGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCGGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GGATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAAGAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCAT CTTCTGACCT GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA TCAACTGAGC TCAAAGAGCC TTCCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TATATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGAAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAGTCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG TACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA GAGGGCACAC AAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTTTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAGTGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247293.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245568.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OSa26710
Clone ID Related Accession (Same CDS sequence)	XM_009247293.2 , XM_009247293.1
Accession Version	XM_009247293.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
ACAGAGCCAG TTCCAGTAGC ACAGCCACAA CCTACCCAGC CGACCACTTT GGCTTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAATGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCATTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTCG TTCATTCTGC TGGAAGACGG TTCATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACATCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTACCCA AATGACAGAA GGACCCAATA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGT ACCAGTCACT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACGG GTGTGGCAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GTGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGAACA TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTCCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTCTG TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAACCTGTT 
GCCAGTGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA ATAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTCTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTGCCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTTACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG ACTGAAACCT 
GGAACTGCTC CCTCAAAGCC ACCTCTAACC AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATTATCACAG TGACTTCTGC GGTTGGTCCT GTGCCCGTGG TGGTTCCAAC AGCAATTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCAGT TGCAGCAGAC 
GATGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCGGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GGATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAAGAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCAT CTTCTGACCT GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA TCAACTGAGC TCAAAGAGCC TTCCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TATATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGAAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAGTCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG TACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA GAGGGCACAC AAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTTTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAGTGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245568.1
CDS	838..7245
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQSIQHPQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247293.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247293.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT 
CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AATCCATACA ACATCCTCAG 
CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG 
ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAACCTCA AGCTCCACAA 
GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT 
ACAGAGCCAG TTCCAGTAGC ACAGCCACAA CCTACCCAGC CGACCACTTT GGCTTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAATGTCCCA 
TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG 
AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC 
AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATAGAGAG AGAGTCATTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC 
AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTCG TTCATTCTGC TGGAAGACGG TTCATAGTGA GTCCTGTGCC AGAAAGCCGA 
TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACATCT 
CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT 
TCTGAACTTA GACGTACCCA AATGACAGAA GGACCCAATA CAGCACCTCC AAACTTTAGT 
CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG CAGCCACAGT ACCAGTCACT GCAACAAGCA GCCCTCCTAA TGACATTTCC 
ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC 
ACCAGCACGG GTGTGGCAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA 
GTACTTTCCA GTGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA 
TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGAACA TTGTTTCTAG TACAGCACTG 
TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA 
GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC 
ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTCCATT 
CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC 
TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA 
TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTCTG TTTCTCAGCC TGGTGGGCTG 
CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA 
CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAACCTGTT 
GCCAGTGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT 
CCCAACCAGC CCCATACTCA TTGTCCTGAA ATAGATTCTG ATACACAACC CAAAGCTCCT 
GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTCTTCAG TGAACACAGC 
TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT 
GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTGCCACA 
AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA 
GTGGTTACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG ACTGAAACCT 
GGAACTGCTC CCTCAAAGCC ACCTCTAACC AAGGCTCCGG TGCTGCCAGT GGGTACTGAA 
CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC 
CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG 
ATTATCACAG TGACTTCTGC GGTTGGTCCT GTGCCCGTGG TGGTTCCAAC AGCAATTACA 
GAAGCAGGAG CACAGCCTCA GAAGGATGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA 
ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCAGT TGCAGCAGAC 
GATGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC 
AGCACTTCAG AGTCCTCGGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA 
GAGCCGAATG GGATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA 
ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG 
GTGACAACTA CAGCAAAGAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC 
ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT 
GTTCTTCCTG CTGTGATACC AAAGAAAGAA AAGCCTGAAC TGTCAGAGCC TTCACATCTA 
AATGGGCCAT CTTCTGACCT GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCA 
GGTAGTCCAC ACTCCCCCCA TCAACTGAGC TCAAAGAGCC TTCCTATCCA GAATCTAAGT 
CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GTGACAATGA GTCAGATATC 
GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT 
CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TATATACCAA ACTGGGCAAG 
GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC 
ACTAAAAGCA AAGGAAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG 
CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC 
CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA 
TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC 
AGCCAAGCCG CCCAGTCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG TACATTCACA 
GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG 
AGAGGAAGCA GAGGGCACAC AAATTATGAG GGCCCTGGAA TGGCAAGGAA GTTTTCTGCA 
CCTGGGCAAC TGTGCATCTC CATGACCTCA AACCTGGGTG GCTCTGCCCC CATCTCTGCA 
GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC 
TTTCCAGCTA CCCCATTTGG CGCTCAGTGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA 
CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG 
CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247294.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245569.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OSa26711
Clone ID Related Accession (Same CDS sequence)	XM_009247294.2 , XM_009247294.1
Accession Version	XM_009247294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009247294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTA CAGAGCCAGT TCCAGTAGCA 
CAGCCACAAC CTACCCAGCC GACCACTTTG GCTTCCTCTA TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AATGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCATTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTCGT TCATTCTGCT 
GGAAGACGGT TCATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACATCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTACCCAA 
ATGACAGAAG GACCCAATAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGTA 
CCAGTCACTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACGGG TGTGGCAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG TGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGAACAT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTCCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTCTGT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAA TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTCTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTGCCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTTACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA CTGAAACCTG GAACTGCTCC CTCAAAGCCA 
CCTCTAACCA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TTATCACAGT GACTTCTGCG 
GTTGGTCCTG TGCCCGTGGT GGTTCCAACA GCAATTACAG AAGCAGGAGC ACAGCCTCAG 
AAGGATGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCAGTT GCAGCAGACG ATGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACTTCAGA GTCCTCGGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG GATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAAGAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAAA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCATC TTCTGACCTG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCAG GTAGTCCACA CTCCCCCCAT 
CAACTGAGCT CAAAGAGCCT TCCTATCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG TGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT ATATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGAAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGGACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAGTCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGT ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAG AGGGCACACA 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTTTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAGTGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245569.1
CDS	635..6961
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247294.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247294.2

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTA CAGAGCCAGT TCCAGTAGCA 
CAGCCACAAC CTACCCAGCC GACCACTTTG GCTTCCTCTA TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AATGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCATTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTCGT TCATTCTGCT 
GGAAGACGGT TCATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACATCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTACCCAA 
ATGACAGAAG GACCCAATAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGTA 
CCAGTCACTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACGGG TGTGGCAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG TGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGAACAT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTCCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTCTGT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAA TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTCTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTGCCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTTACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA CTGAAACCTG GAACTGCTCC CTCAAAGCCA 
CCTCTAACCA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TTATCACAGT GACTTCTGCG 
GTTGGTCCTG TGCCCGTGGT GGTTCCAACA GCAATTACAG AAGCAGGAGC ACAGCCTCAG 
AAGGATGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCAGTT GCAGCAGACG ATGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACTTCAGA GTCCTCGGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG GATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAAGAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAAA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCATC TTCTGACCTG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCAG GTAGTCCACA CTCCCCCCAT 
CAACTGAGCT CAAAGAGCCT TCCTATCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG TGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT ATATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGAAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGGACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAGTCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGT ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAG AGGGCACACA 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTTTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAGTGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247294.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245569.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OSa26711
Clone ID Related Accession (Same CDS sequence)	XM_009247294.2 , XM_009247294.1
Accession Version	XM_009247294.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885634.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTA CAGAGCCAGT TCCAGTAGCA 
CAGCCACAAC CTACCCAGCC GACCACTTTG GCTTCCTCTA TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AATGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCATTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTCGT TCATTCTGCT 
GGAAGACGGT TCATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACATCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTACCCAA 
ATGACAGAAG GACCCAATAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGTA 
CCAGTCACTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACGGG TGTGGCAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG TGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGAACAT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTCCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTCTGT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAA TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTCTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTGCCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTTACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA CTGAAACCTG GAACTGCTCC CTCAAAGCCA 
CCTCTAACCA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TTATCACAGT GACTTCTGCG 
GTTGGTCCTG TGCCCGTGGT GGTTCCAACA GCAATTACAG AAGCAGGAGC ACAGCCTCAG 
AAGGATGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCAGTT GCAGCAGACG ATGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACTTCAGA GTCCTCGGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG GATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAAGAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAAA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCATC TTCTGACCTG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCAG GTAGTCCACA CTCCCCCCAT 
CAACTGAGCT CAAAGAGCCT TCCTATCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG TGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT ATATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGAAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGGACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAGTCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGT ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAG AGGGCACACA 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTTTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAGTGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245569.1
CDS	838..7164
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQSTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247294.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247294.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA 
GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC 
ACTACTGCAC AGCCAGTGAG TCAACCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT 
GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTA CAGAGCCAGT TCCAGTAGCA 
CAGCCACAAC CTACCCAGCC GACCACTTTG GCTTCCTCTA TAGACAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGCAATGAG AATGTCCCAT CTTCCAGTGG AAGGCATGAA 
GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA 
ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA 
TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC 
AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA 
GAGTCATTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTCAGTG TGGAACCAGA GGGTGACCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC 
TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT 
TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTCGT TCATTCTGCT 
GGAAGACGGT TCATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC 
CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACATCTC AGAGCCCTGG AATGAACTTG 
TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTACCCAA 
ATGACAGAAG GACCCAATAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA 
GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGTA 
CCAGTCACTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG 
GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACGGG TGTGGCAACT 
TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG TGTAGTTTCA 
AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC 
ACATCCACAT CTGAGAACAT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA 
GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA 
GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC 
ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTCCATTC AGCTTAGCAG CAGTACTTCT 
ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA 
GCAGGAGTGT CTAGTTCTGT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA 
GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA 
CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT 
TGTCCTGAAA TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAAGAAA AGCTGCGGTC TCTCTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC 
TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT 
ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTGCCACAA GTACTTGCTT ACCACCAACC 
AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTTACACC TGGGCAAGTT 
TCTACCCCAG TCAGCACTAC TACATCAGGA CTGAAACCTG GAACTGCTCC CTCAAAGCCA 
CCTCTAACCA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC 
AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG 
GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TTATCACAGT GACTTCTGCG 
GTTGGTCCTG TGCCCGTGGT GGTTCCAACA GCAATTACAG AAGCAGGAGC ACAGCCTCAG 
AAGGATGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT 
TTTAAGATGG GACGATTTCA GGTTTCAGTT GCAGCAGACG ATGCCCAGAA AGAGGGTAAA 
AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACTTCAGA GTCCTCGGTG 
CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG GATAACCATC 
CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG 
TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAAGAAA 
GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA 
CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA 
AAGAAAGAAA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCATC TTCTGACCTG 
GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCAG GTAGTCCACA CTCCCCCCAT 
CAACTGAGCT CAAAGAGCCT TCCTATCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT 
AACTCCTCTT ACATGAGTAG TGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA 
GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG 
AAGCATGAAA TTGAATCTTT ATATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT 
CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGAAGCAAA 
TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA 
GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC 
TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGGACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAGTCTCAG 
CCTCCTGCCA TGACGTCCAG CAGGAAGGGT ACATTCACAG ATGACTTGCA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAG AGGGCACACA 
AATTATGAGG GCCCTGGAAT GGCAAGGAAG TTTTCTGCAC CTGGGCAACT GTGCATCTCC 
ATGACCTCAA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC 
GCTCAGTGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG 
GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGCTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_009247295.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009245570.1 Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OSa26712
Clone ID Related Accession (Same CDS sequence)	XM_009247295.1
Accession Version	XM_009247295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACCTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAT CTTCAGAATC TAAGATTAGA TTCTGGATTG 
AGTCCAGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTATATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCATCTTT TGGCTCTGAC 
GTCTCATTGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ATCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAACCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009245570.1
CDS	838..8766
Translation	MSGGAAEKQSGTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSVSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHLQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLSPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLYGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAASFGSDVSLPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQAQGQSQGQPSSSSLTGVSSSQSIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQGFPPRLPPQYPGDSNIAPSSHVASVCTHPTVLSPPMPTEALAAPGLFPTVAQPYVESNLLVPVGAVGGQVQVSQPGVSLAQAPTTSSQQAVLESTQGVSQVAPTEPVPVAQPQPTQPTTLASSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTSQSPGMNLSHSASSLSLQQAFSELRRTQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATVPVTATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVATSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSENIVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSSVSQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSATSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGLKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEIITVTSAVGPVPVVVPTAITEAGAQPQKDVSQVKEGPVLATSSGAGVFKMGRFQVSVAADDAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTAKKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQSQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSRGHTNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009247295.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247295.1

ORF Insert Sequence:

ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC GGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAGCTGGGA 
GCCGCGGCCG CCGACGCTGT GGCCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT 
ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC 
CGCCGGAGTG TCATCTGCGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT 
TCCCTCCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC 
CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT 
GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC 
AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG 
GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCGC AGGAGGAACG GAGCCAGCAG 
CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT 
CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTAGAC 
ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT 
GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT 
GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG 
ACTGAACTTA TGACATCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG 
ATCAAAGTCC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GACTTCAGTT TCTTCATACT 
CGAACTCCAC CTATCATTCA CCGTGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT 
ACTGGCTCAG TCAAGATTGG AGACCTTGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC 
AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT 
GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT 
CCTTACTCGG AGTGCCAAAA TGCTGCGCAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG 
CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATCATTGA AGGATGCATA 
CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA 
GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGACG ATGGAGAAAA AATAGCCATA 
AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA 
GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC TCAAGAAATG 
GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC 
AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA 
AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG 
ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACAGCTTC TACCACTTCA 
GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA 
CAGTACCAGC AACCCAGTGT ATCTGTGTTA TCTGATGGGA CAGTTGACAG TGGTCAGGGA 
TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAG 
CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA 
CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG 
TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT 
CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT 
AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACCTCC AACCCCTGCT GAGGACTGTT 
GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT 
ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT 
ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA 
GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG 
GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA 
CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT 
CCATCTGGAC AGGCTTTCCT GGTAGGACAT CTTCAGAATC TAAGATTAGA TTCTGGATTG 
AGTCCAGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG 
AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG 
AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA 
GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT 
CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTATATGGCT ACTTGCAGCC TGTGACTGAA 
GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG 
AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC 
CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCATCTTT TGGCTCTGAC 
GTCTCATTGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC 
TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA 
TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA 
ACAGGGGTTT CATCTTCCCA ATCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG 
ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG 
GCCACTACTG CACAGCCAGT GAGTCAACCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA 
GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT 
GCTCCCTCTT CCCACGTGGC TTCTGTTTGC ACCCACCCTA CAGTCCTGTC CCCTCCCATG 
CCGACAGAAG CACTGGCTGC ACCTGGGCTC TTTCCCACGG TGGCGCAGCC TTATGTGGAA 
TCAAATCTTT TAGTTCCTGT GGGTGCTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA 
GTGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG 
GGAGTCTCTC AGGTTGCTCC TACAGAGCCA GTTCCAGTAG CACAGCCACA ACCTACCCAG 
CCGACCACTT TGGCTTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT 
GATGGCAATG AGAATGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG 
CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT 
AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCATT TGTGGATCAA 
GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT 
CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTC GTTCATTCTG CTGGAAGACG GTTCATAGTG 
AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT 
ACAGTTGCTG CCTCTACATC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC 
CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTACCC AAATGACAGA AGGACCCAAT 
ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG TACCAGTCAC TGCAACAAGC 
AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA 
GAGGGGATTG CTGGAGTTGC CACCAGCACG GGTGTGGCAA CTTCAGGTGG TCTCCCCATA 
CCACCTGTGT CTGAATCACC AGTACTTTCC AGTGTAGTTT CAAGTATCAC AATACCTGCA 
GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGAAC 
ATTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA 
GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA 
GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC 
ACAGCTTCAC AGCTGTCCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA 
ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTCT 
GTTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT 
ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC 
CCACCCTTGG TACAACCTGT TGCCAGTGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AATAGATTCT 
GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG 
TCTCTCTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC 
TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC 
AAACTCCTGA CTTCTGCCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA 
GTTGCTTTGC CAGTTACACC AGTGGTTACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT 
ACTACATCAG GACTGAAACC TGGAACTGCT CCCTCAAAGC CACCTCTAAC CAAGGCTCCG 
GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT 
TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG 
AGAAGAACAC TTAGTCCAGA GATTATCACA GTGACTTCTG CGGTTGGTCC TGTGCCCGTG 
GTGGTTCCAA CAGCAATTAC AGAAGCAGGA GCACAGCCTC AGAAGGATGT TTCTCAAGTC 
AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT 
CAGGTTTCAG TTGCAGCAGA CGATGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA 
AAGTCTGTTC ATTTTGAATC CAGCACTTCA GAGTCCTCGG TGCTATCAAG TAGTAGTCCA 
GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGGATAACCA TCCCTGGTAT CTCTTCAGAT 
GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT 
ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAAGA AAGTGGGTCG TTTCTCTGTA 
TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT 
TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA AAAGCCTGAA 
CTGTCAGAGC CTTCACATCT AAATGGGCCA TCTTCTGACC TGGAGGCCGC TTTTTTAAGT 
AGGGATGTGG ATGATGGTTC AGGTAGTCCA CACTCCCCCC ATCAACTGAG CTCAAAGAGC 
CTTCCTATCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT 
AGTGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA 
GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT 
TTATATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT 
TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGAAGCA AATCTAGTCG AAGCAGTTCC 
TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC 
TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG 
CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT 
GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGCC AAGGGACCAG CAGCACAAAC 
ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAGTCTC AGCCTCCTGC CATGACGTCC 
AGCAGGAAGG GTACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AGAGGGCACA CAAATTATGA GGGCCCTGGA 
ATGGCAAGGA AGTTTTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC AAACCTGGGT 
GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAGTG GAGTGGGACG 
GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK1
Entrez Gene ID	100436057
Full Name	WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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