NEWGENE_621351 cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following NEWGENE_621351 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEWGENE_621351 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa52082 XM_008775673.2
Latest version!
Rattus norvegicus collagen, type I, alpha 2 (NEWGENE_621351), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa52083 XM_008762736.1
Latest version!
Rattus norvegicus collagen, type I, alpha 2 (NEWGENE_621351), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa52082
    Clone ID Related Accession (Same CDS sequence) XM_008775673.2
    Accession Version XM_008775673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-2(I) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000072.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAC GCGAACTCTG TTGCTGCTTG CAGTAACGTC GTGCCTAGCA 
    ACATGCCAAT CTTTACAAAT GGGATCTGTA CGGAAGGGCC CCACTGGAGA CAGAGGACCG
    CGTGGACAAA GGGGCCCAGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCGTTGGC
    CCTCCTGGTC CCCCTGGTGC CCCTGGCCCC CCTGGTCCCC CTGGCCCCCC TGGTCTTACT
    GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAG CTGGCCCCGG ACCAATGGGC
    TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTCGGAGCCC CTGGCCCTCA AGGTTTCCAA
    GGACCTGCTG GTGAGCCTGG TGAGCCCGGA CAAACGGGTC CTGCAGGTTC CCGTGGGCCA
    GCTGGCCCTC CTGGCAAGGC CGGTGAAGAT GGTCACCCTG GAAAACCTGG AAGACCTGGC
    GAGAGAGGAG TCGTTGGGCC ACAGGGTGCT CGTGGTTTCC CTGGGACTCC TGGACTTCCT
    GGCTTCAAAG GCATTCGAGG ACACAACGGT CTGGATGGAT TGAAAGGACA GCCTGGTGCC
    CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACCCCAGG TCAAGCAGGA
    GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGT
    GGCAGTGATG GCAGTGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGTCCT
    CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCTGTTGG TAACCCTGGC
    CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GCGGGTCTTC CGGGTCTTTC TGGCCCCGTT
    GGACCTCCTG GCAACCCTGG AGCAAATGGC CTCACTGGTG CCAAGGGAGC CACTGGACTC
    CCTGGTGTTG CTGGGGCTCC TGGTCTCCCC GGTCCCCGTG GTATTCCTGG CCCCGTTGGT
    GCTGCTGGTG CTACTGGTCC CAGAGGACTT GTTGGTGAAC CTGGTCCTGC TGGCTCCAAA
    GGAGAGACTG GTAACAAGGG TGAGCCTGGC TCTGCTGGAG CCCAAGGTCC TCCTGGTCCC
    AGCGGTGAAG AAGGAAAGAG AGGGTCCCCT GGAGAACCTG GATCTGCTGG TCCTGCAGGG
    CCTCCAGGGC TGAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT
    GGTGTGATGG GCCCTCCTGG CAATCGTGGT TCAACTGGCC CTGCTGGAGT CCGAGGTCCT
    AATGGAGATG CCGGTCGCCC TGGAGAACCT GGTCTCATGG GACCCAGAGG TCTTCCCGGG
    TCTCCTGGAA ATGTTGGCCC AGCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATCGAT
    GGCAGACCTG GCCCAATTGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC
    CCTGGACCCA AAGGCCCCTC TGGAGCTCCA GGTCCCGACG GCAACAATGG TGCTCAGGGC
    CCTCCTGGAC CTCAGGGTGT TCAAGGTGGC AAAGGTGAAC AGGGCCCTGC TGGTCCTCCA
    GGCTTCCAGG GTCTCCCGGG TCCCTCAGGT ACCGCTGGAG AAGTTGGCAA GCCAGGAGAA
    AGGGGTCTTC CCGGTGAATT CGGTCTCCCT GGTCCTGCTG GTCCAAGAGG AGAACGTGGT
    CCCCCAGGTG AGAGTGGAGC TGCTGGTCCT TCTGGTCCTA TTGGAAGCCG AGGTCCCTCT
    GGAGCCCCAG GGCCTGATGG GAACAAGGGT GAAGCTGGTG CAGTCGGTGC TCCAGGCTCT
    GCTGGTGCCT CTGGTCCTGG TGGGCTTCCA GGAGAGAGGG GTGCTGCTGG CATACCTGGA
    GGCAAAGGAG AAAAGGGTGA AACTGGTCTC AGAGGTGAAA TCGGCAACCC TGGTAGAGAT
    GGTGCTCGAG GTGCTCCTGG TGCTATAGGT GCCCCTGGTC CCGCTGGAGC CTCAGGTGAC
    CGGGGCGAAG CTGGTGCTGC TGGTCCTTCT GGCCCTGCTG GTCCTCGAGG TAGCCCTGGT
    GAACGTGGTG AGGTTGGTCC TGCTGGCCCC AATGGATTTG CTGGTCCTGC TGGTTCTGCT
    GGCCAACCTG GTGCTAAAGG AGAAAAGGGA ACCAAAGGGC CTAAGGGAGA AAATGGCATC
    GTTGGTCCAA CCGGACCTGT CGGAGCTGCT GGCCCATCTG GTCCTAATGG CCCACCTGGC
    CCTGCTGGAA GTCGTGGTGA TGGAGGGCCC CCTGGTATGA CTGGCTTCCC TGGTGCTGCT
    GGACGGACTG GTCCCCCCGG ACCCAGTGGT ATTACTGGCC CCCCTGGTCC CCCTGGTGCT
    GCTGGTAAAG AAGGTATTCG TGGTCCCCGT GGTGACCAAG GTCCAGTTGG CCGAACTGGA
    GAAATAGGTG CATCTGGTCC CCCTGGATTT GCTGGCGAGA AGGGTCCTTC TGGAGAACCT
    GGTACCACAG GACCCCCTGG CACTGCAGGT CCTCAGGGTC TTCTTGGTGC TCCCGGTATT
    CTGGGTCTCC CTGGCTCTCG AGGTGAACGT GGCCTGCCAG GTATTGCTGG TGCTCTGGGT
    GAACCTGGTC CTCTGGGCAT TGCAGGCCCT CCTGGGGCCC GTGGTCCCCC TGGTGCTGTG
    GGCAGTCCTG GAGTTAATGG TGCCCCTGGT GAAGCCGGTC GTGATGGTAA CCCTGGCAGT
    GATGGTCCCC CAGGTCGTGA TGGTCAACCC GGACACAAGG GAGAGCGTGG TTACCCTGGC
    AACATTGGTC CCACTGGTGC TGCAGGTGCT CCTGGTCCTC ATGGTTCTGT GGGTCCTGCT
    GGGAAACATG GAAACCGTGG TGAGCCTGGT CCTGCTGGTT CTGTTGGTCC CGTTGGTGCT
    GTCGGTCCAA GAGGTCCTAG TGGTCCTCAA GGTATTCGAG GTGACAAGGG AGAGCCTGGT
    GACAAAGGGG CCAGAGGTCT TCCTGGCTTA AAAGGACACA ATGGATTACA GGGTCTTCCT
    GGTCTTGCTG GGCTGCATGG TGACCAAGGA GCTCCTGGTC CCGTGGGTCC TGCTGGTCCC
    AGGGGTCCTG CTGGTCCTTC TGGCCCCATT GGCAAAGATG GTAGATCTGG ACATCCTGGA
    CCAGTCGGTC CTGCTGGCGT TCGTGGCTCT CAGGGTAGCC AAGGTCCCGC TGGTCCCCCT
    GGTCCCCCTG GGCCTCCTGG GCCTCCTGGT GTCAGCGGAG GAGGCTATGA CTTTGGTTTC
    GAAGGAGACT TCTACAGGGC TGACCAGCCT CGCTCACAGC CTTCACTCAG ACCCAAGGAC
    TATGAAGTTG ATGCAACTCT GAAATCTCTC AATAACCAAA TCGAGACCCT TCTCACTCCT
    GAAGGCTCTA GAAAGAACCC TGCCCGCACA TGCCGTGACT TAAGACTCAG CCACCCAGAG
    TGGAAGAGCG ATTACTACTG GATTGACCCT AACCAAGGAT GCACTATGGA TGCCATCAAA
    GTGTACTGCG ATTTCTCTAC TGGTGAAACC TGCATCCAGG CCCAACCTGT CAACACCCCA
    GCCAAGAATG CATACAGCCG TGCCCAGGCC AACAAGCATG TCTGGTTAGG AGAGACCATC
    AATGGTGGCA GCCAGTTTGA ATACAACGCA GAAGGGGTGT CCTCCAAGGA AATGGCAACT
    CAGCTCGCCT TCATGCGCCT GCTAGCCAAC CGTGCTTCTC AGAACATCAC CTACCACTGC
    AAGAACAGCA TTGCGTACCT GGACGAGGAG ACAGGCCGCC TGAATAAGGC TGTCATTCTG
    CAGGGCTCCA ACGACGTCGA ACTTGTTGCT GAGGGCAACA GCAGATTCAC CTACACTGTC
    CTTGTCGATG GCTGCTCCAA AAAGACAAAT GAATGGGACA AGACAATCAT TGAATACAAA
    ACGAATAAGC CATCTCGCCT GCCATTCCTT GACATTGCAC CTCTGGACAT CGGTGGTACT
    AACCAAGAAT TCCGTGTGGA GGTTGGCCCT GTCTGTTTCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008773895.1
    CDS351..4415
    Translation

    Target ORF information:

    RefSeq Version XM_008775673.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen, type I, alpha 2 (NEWGENE_621351), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008775673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCTCAGCT TTGTGGATAC GCGAACTCTG TTGCTGCTTG CAGTAACGTC GTGCCTAGCA 
    ACATGCCAAT CTTTACAAAT GGGATCTGTA CGGAAGGGCC CCACTGGAGA CAGAGGACCG
    CGTGGACAAA GGGGCCCAGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCGTTGGC
    CCTCCTGGTC CCCCTGGTGC CCCTGGCCCC CCTGGTCCCC CTGGCCCCCC TGGTCTTACT
    GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAG CTGGCCCCGG ACCAATGGGC
    TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTCGGAGCCC CTGGCCCTCA AGGTTTCCAA
    GGACCTGCTG GTGAGCCTGG TGAGCCCGGA CAAACGGGTC CTGCAGGTTC CCGTGGGCCA
    GCTGGCCCTC CTGGCAAGGC CGGTGAAGAT GGTCACCCTG GAAAACCTGG AAGACCTGGC
    GAGAGAGGAG TCGTTGGGCC ACAGGGTGCT CGTGGTTTCC CTGGGACTCC TGGACTTCCT
    GGCTTCAAAG GCATTCGAGG ACACAACGGT CTGGATGGAT TGAAAGGACA GCCTGGTGCC
    CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACCCCAGG TCAAGCAGGA
    GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGT
    GGCAGTGATG GCAGTGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGTCCT
    CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCTGTTGG TAACCCTGGC
    CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GCGGGTCTTC CGGGTCTTTC TGGCCCCGTT
    GGACCTCCTG GCAACCCTGG AGCAAATGGC CTCACTGGTG CCAAGGGAGC CACTGGACTC
    CCTGGTGTTG CTGGGGCTCC TGGTCTCCCC GGTCCCCGTG GTATTCCTGG CCCCGTTGGT
    GCTGCTGGTG CTACTGGTCC CAGAGGACTT GTTGGTGAAC CTGGTCCTGC TGGCTCCAAA
    GGAGAGACTG GTAACAAGGG TGAGCCTGGC TCTGCTGGAG CCCAAGGTCC TCCTGGTCCC
    AGCGGTGAAG AAGGAAAGAG AGGGTCCCCT GGAGAACCTG GATCTGCTGG TCCTGCAGGG
    CCTCCAGGGC TGAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT
    GGTGTGATGG GCCCTCCTGG CAATCGTGGT TCAACTGGCC CTGCTGGAGT CCGAGGTCCT
    AATGGAGATG CCGGTCGCCC TGGAGAACCT GGTCTCATGG GACCCAGAGG TCTTCCCGGG
    TCTCCTGGAA ATGTTGGCCC AGCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATCGAT
    GGCAGACCTG GCCCAATTGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC
    CCTGGACCCA AAGGCCCCTC TGGAGCTCCA GGTCCCGACG GCAACAATGG TGCTCAGGGC
    CCTCCTGGAC CTCAGGGTGT TCAAGGTGGC AAAGGTGAAC AGGGCCCTGC TGGTCCTCCA
    GGCTTCCAGG GTCTCCCGGG TCCCTCAGGT ACCGCTGGAG AAGTTGGCAA GCCAGGAGAA
    AGGGGTCTTC CCGGTGAATT CGGTCTCCCT GGTCCTGCTG GTCCAAGAGG AGAACGTGGT
    CCCCCAGGTG AGAGTGGAGC TGCTGGTCCT TCTGGTCCTA TTGGAAGCCG AGGTCCCTCT
    GGAGCCCCAG GGCCTGATGG GAACAAGGGT GAAGCTGGTG CAGTCGGTGC TCCAGGCTCT
    GCTGGTGCCT CTGGTCCTGG TGGGCTTCCA GGAGAGAGGG GTGCTGCTGG CATACCTGGA
    GGCAAAGGAG AAAAGGGTGA AACTGGTCTC AGAGGTGAAA TCGGCAACCC TGGTAGAGAT
    GGTGCTCGAG GTGCTCCTGG TGCTATAGGT GCCCCTGGTC CCGCTGGAGC CTCAGGTGAC
    CGGGGCGAAG CTGGTGCTGC TGGTCCTTCT GGCCCTGCTG GTCCTCGAGG TAGCCCTGGT
    GAACGTGGTG AGGTTGGTCC TGCTGGCCCC AATGGATTTG CTGGTCCTGC TGGTTCTGCT
    GGCCAACCTG GTGCTAAAGG AGAAAAGGGA ACCAAAGGGC CTAAGGGAGA AAATGGCATC
    GTTGGTCCAA CCGGACCTGT CGGAGCTGCT GGCCCATCTG GTCCTAATGG CCCACCTGGC
    CCTGCTGGAA GTCGTGGTGA TGGAGGGCCC CCTGGTATGA CTGGCTTCCC TGGTGCTGCT
    GGACGGACTG GTCCCCCCGG ACCCAGTGGT ATTACTGGCC CCCCTGGTCC CCCTGGTGCT
    GCTGGTAAAG AAGGTATTCG TGGTCCCCGT GGTGACCAAG GTCCAGTTGG CCGAACTGGA
    GAAATAGGTG CATCTGGTCC CCCTGGATTT GCTGGCGAGA AGGGTCCTTC TGGAGAACCT
    GGTACCACAG GACCCCCTGG CACTGCAGGT CCTCAGGGTC TTCTTGGTGC TCCCGGTATT
    CTGGGTCTCC CTGGCTCTCG AGGTGAACGT GGCCTGCCAG GTATTGCTGG TGCTCTGGGT
    GAACCTGGTC CTCTGGGCAT TGCAGGCCCT CCTGGGGCCC GTGGTCCCCC TGGTGCTGTG
    GGCAGTCCTG GAGTTAATGG TGCCCCTGGT GAAGCCGGTC GTGATGGTAA CCCTGGCAGT
    GATGGTCCCC CAGGTCGTGA TGGTCAACCC GGACACAAGG GAGAGCGTGG TTACCCTGGC
    AACATTGGTC CCACTGGTGC TGCAGGTGCT CCTGGTCCTC ATGGTTCTGT GGGTCCTGCT
    GGGAAACATG GAAACCGTGG TGAGCCTGGT CCTGCTGGTT CTGTTGGTCC CGTTGGTGCT
    GTCGGTCCAA GAGGTCCTAG TGGTCCTCAA GGTATTCGAG GTGACAAGGG AGAGCCTGGT
    GACAAAGGGG CCAGAGGTCT TCCTGGCTTA AAAGGACACA ATGGATTACA GGGTCTTCCT
    GGTCTTGCTG GGCTGCATGG TGACCAAGGA GCTCCTGGTC CCGTGGGTCC TGCTGGTCCC
    AGGGGTCCTG CTGGTCCTTC TGGCCCCATT GGCAAAGATG GTAGATCTGG ACATCCTGGA
    CCAGTCGGTC CTGCTGGCGT TCGTGGCTCT CAGGGTAGCC AAGGTCCCGC TGGTCCCCCT
    GGTCCCCCTG GGCCTCCTGG GCCTCCTGGT GTCAGCGGAG GAGGCTATGA CTTTGGTTTC
    GAAGGAGACT TCTACAGGGC TGACCAGCCT CGCTCACAGC CTTCACTCAG ACCCAAGGAC
    TATGAAGTTG ATGCAACTCT GAAATCTCTC AATAACCAAA TCGAGACCCT TCTCACTCCT
    GAAGGCTCTA GAAAGAACCC TGCCCGCACA TGCCGTGACT TAAGACTCAG CCACCCAGAG
    TGGAAGAGCG ATTACTACTG GATTGACCCT AACCAAGGAT GCACTATGGA TGCCATCAAA
    GTGTACTGCG ATTTCTCTAC TGGTGAAACC TGCATCCAGG CCCAACCTGT CAACACCCCA
    GCCAAGAATG CATACAGCCG TGCCCAGGCC AACAAGCATG TCTGGTTAGG AGAGACCATC
    AATGGTGGCA GCCAGTTTGA ATACAACGCA GAAGGGGTGT CCTCCAAGGA AATGGCAACT
    CAGCTCGCCT TCATGCGCCT GCTAGCCAAC CGTGCTTCTC AGAACATCAC CTACCACTGC
    AAGAACAGCA TTGCGTACCT GGACGAGGAG ACAGGCCGCC TGAATAAGGC TGTCATTCTG
    CAGGGCTCCA ACGACGTCGA ACTTGTTGCT GAGGGCAACA GCAGATTCAC CTACACTGTC
    CTTGTCGATG GCTGCTCCAA AAAGACAAAT GAATGGGACA AGACAATCAT TGAATACAAA
    ACGAATAAGC CATCTCGCCT GCCATTCCTT GACATTGCAC CTCTGGACAT CGGTGGTACT
    AACCAAGAAT TCCGTGTGGA GGTTGGCCCT GTCTGTTTCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa52083
    Clone ID Related Accession (Same CDS sequence) XM_008762736.1
    Accession Version XM_008762736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-2(I) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTCAGCT TTGTGGATAC GCGAACTCTG TTGCTGCTTG CAGTAACGTC GTGCCTAGCA 
    ACATGCCAAT CTTTACAAAT GGGATCTGTA CGGAAGGGCC CCACTGGAGA CAGAGGACCG
    CGTGGACAAA GGGGCCCAGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCGTTGGC
    CCTCCTGGTC CCCCTGGTGC CCCTGGCCCC CCTGGTCCCC CTGGCCCCCC TGGTCTTACT
    GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAG CTGGCCCCGG ACCAATGGGC
    TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTCGGAGCCC CTGGCCCTCA AGGTTTCCAA
    GGACCTGCTG GTGAGCCTGG TGAGCCCGGA CAAACGGGTC CTGCAGGTTC CCGTGGGCCA
    GCTGGCCCTC CTGGCAAGGC CGGTGAAGAT GGTCACCCTG GAAAACCTGG AAGACCTGGC
    GAGAGAGGAG TCGTTGGGCC ACAGGGTGCT CGTGGTTTCC CTGGGACTCC TGGACTTCCT
    GGCTTCAAAG GCATTCGAGG ACACAACGGT CTGGATGGAT TGAAAGGACA GCCTGGTGCC
    CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACCCCAGG TCAAGCAGGA
    GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGT
    GGCAGTGATG GCAGTGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGTCCT
    CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCTGTTGG TAACCCTGGC
    CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GCGGGTCTTC CGGGTCTTTC TGGCCCCGTT
    GGACCTCCTG GCAACCCTGG AGCAAATGGC CTCACTGGTG CCAAGGGAGC CACTGGACTC
    CCTGGTGTTG CTGGGGCTCC TGGTCTCCCC GGTCCCCGTG GTATTCCTGG CCCCGTTGGT
    GCTGCTGGTG CTACTGGTCC CAGAGGACTT GTTGGTGAAC CTGGTCCTGC TGGCTCCAAA
    GGAGAGACTG GTAACAAGGG TGAGCCTGGC TCTGCTGGAG CCCAAGGTCC TCCTGGTCCC
    AGCGGTGAAG AAGGAAAGAG AGGGTCCCCT GGAGAACCTG GATCTGCTGG TCCTGCAGGG
    CCTCCAGGGC TGAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT
    GGTGTGATGG GCCCTCCTGG CAATCGTGGT TCAACTGGCC CTGCTGGAGT CCGAGGTCCT
    AATGGAGATG CCGGTCGCCC TGGAGAACCT GGTCTCATGG GACCCAGAGG TCTTCCCGGG
    TCTCCTGGAA ATGTTGGCCC AGCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATCGAT
    GGCAGACCTG GCCCAATTGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC
    CCTGGACCCA AAGGCCCCTC TGGTGATCCT GGCAAACCTG GTGAGAAAGG CCATCCCGGT
    CTCGCTGGTG CTCGGGGAGC TCCAGGTCCC GACGGCAACA ATGGTGCTCA GGGCCCTCCT
    GGACCTCAGG GTGTTCAAGG TGGCAAAGGT GAACAGGGCC CTGCTGGTCC TCCAGGCTTC
    CAGGGTCTCC CGGGTCCCTC AGGTACCGCT GGAGAAGTTG GCAAGCCAGG AGAAAGGGGT
    CTTCCCGGTG AATTCGGTCT CCCTGGTCCT GCTGGTCCAA GAGGAGAACG TGGTCCCCCA
    GGTGAGAGTG GAGCTGCTGG TCCTTCTGGT CCTATTGGAA GCCGAGGTCC CTCTGGAGCC
    CCAGGGCCTG ATGGGAACAA GGGTGAAGCT GGTGCAGTCG GTGCTCCAGG CTCTGCTGGT
    GCCTCTGGTC CTGGTGGGCT TCCAGGAGAG AGGGGTGCTG CTGGCATACC TGGAGGCAAA
    GGAGAAAAGG GTGAAACTGG TCTCAGAGGT GAAATCGGCA ACCCTGGTAG AGATGGTGCT
    CGAGGTGCTC CTGGTGCTAT AGGTGCCCCT GGTCCCGCTG GAGCCTCAGG TGACCGGGGC
    GAAGCTGGTG CTGCTGGTCC TTCTGGCCCT GCTGGTCCTC GAGGTAGCCC TGGTGAACGT
    GGTGAGGTTG GTCCTGCTGG CCCCAATGGA TTTGCTGGTC CTGCTGGTTC TGCTGGCCAA
    CCTGGTGCTA AAGGAGAAAA GGGAACCAAA GGGCCTAAGG GAGAAAATGG CATCGTTGGT
    CCAACCGGAC CTGTCGGAGC TGCTGGCCCA TCTGGTCCTA ATGGCCCACC TGGCCCTGCT
    GGAAGTCGTG GTGATGGAGG GCCCCCTGGT ATGACTGGCT TCCCTGGTGC TGCTGGACGG
    ACTGGTCCCC CCGGACCCAG TGGTATTACT GGCCCCCCTG GTCCCCCTGG TGCTGCTGGT
    AAAGAAGGTA TTCGTGGTCC CCGTGGTGAC CAAGGTCCAG TTGGCCGAAC TGGAGAAATA
    GGTGCATCTG GTCCCCCTGG ATTTGCTGGC GAGAAGGGTC CTTCTGGAGA ACCTGGTACC
    ACAGGACCCC CTGGCACTGC AGGTCCTCAG GGTCTTCTTG GTGCTCCCGG TATTCTGGGT
    CTCCCTGGCT CTCGAGGTGA ACGTGGCCTG CCAGGTATTG CTGGTGCTCT GGGTGAACCT
    GGTCCTCTGG GCATTGCAGG CCCTCCTGGG GCCCGTGGTC CCCCTGGTGC TGTGGGCAGT
    CCTGGAGTTA ATGGTGCCCC TGGTGAAGCC GGTCGTGATG GTAACCCTGG CAGTGATGGT
    CCCCCAGGTC GTGATGGTCA ACCCGGACAC AAGGGAGAGC GTGGTTACCC TGGCAACATT
    GGTCCCACTG GTGCTGCAGG TGCTCCTGGT CCTCATGGTT CTGTGGGTCC TGCTGGGAAA
    CATGGAAACC GTGGTGAGCC TGGTCCTGCT GGTTCTGTTG GTCCCGTTGG TGCTGTCGGT
    CCAAGAGGTC CTAGTGGTCC TCAAGGTATT CGAGGTGACA AGGGAGAGCC TGGTGACAAA
    GGGGCCAGAG GTCTTCCTGG CTTAAAAGGA CACAATGGAT TACAGGGTCT TCCTGGTCTT
    GCTGGGCTGC ATGGTGACCA AGGAGCTCCT GGTCCCGTGG GTCCTGCTGG TCCCAGGGGT
    CCTGCTGGTC CTTCTGGCCC CATTGGCAAA GATGGTAGAT CTGGACATCC TGGACCAGTC
    GGTCCTGCTG GCGTTCGTGG CTCTCAGGGT AGCCAAGGTC CCGCTGGTCC CCCTGGTCCC
    CCTGGGCCTC CTGGGCCTCC TGGTGTCAGC GGAGGAGGCT ATGACTTTGG TTTCGAAGGA
    GACTTCTACA GGGCTGACCA GCCTCGCTCA CAGCCTTCAC TCAGACCCAA GGACTATGAA
    GTTGATGCAA CTCTGAAATC TCTCAATAAC CAAATCGAGA CCCTTCTCAC TCCTGAAGGC
    TCTAGAAAGA ACCCTGCCCG CACATGCCGT GACTTAAGAC TCAGCCACCC AGAGTGGAAG
    AGCGATTACT ACTGGATTGA CCCTAACCAA GGATGCACTA TGGATGCCAT CAAAGTGTAC
    TGCGATTTCT CTACTGGTGA AACCTGCATC CAGGCCCAAC CTGTCAACAC CCCAGCCAAG
    AATGCATACA GCCGTGCCCA GGCCAACAAG CATGTCTGGT TAGGAGAGAC CATCAATGGT
    GGCAGCCAGT TTGAATACAA CGCAGAAGGG GTGTCCTCCA AGGAAATGGC AACTCAGCTC
    GCCTTCATGC GCCTGCTAGC CAACCGTGCT TCTCAGAACA TCACCTACCA CTGCAAGAAC
    AGCATTGCGT ACCTGGACGA GGAGACAGGC CGCCTGAATA AGGCTGTCAT TCTGCAGGGC
    TCCAACGACG TCGAACTTGT TGCTGAGGGC AACAGCAGAT TCACCTACAC TGTCCTTGTC
    GATGGCTGCT CCAAAAAGAC AAATGAATGG GACAAGACAA TCATTGAATA CAAAACGAAT
    AAGCCATCTC GCCTGCCATT CCTTGACATT GCACCTCTGG ACATCGGTGG TACTAACCAA
    GAATTCCGTG TGGAGGTTGG CCCTGTCTGT TTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008760958.1
    CDS351..4469
    Translation

    Target ORF information:

    RefSeq Version XM_008762736.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen, type I, alpha 2 (NEWGENE_621351), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008762736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCTCAGCT TTGTGGATAC GCGAACTCTG TTGCTGCTTG CAGTAACGTC GTGCCTAGCA 
    ACATGCCAAT CTTTACAAAT GGGATCTGTA CGGAAGGGCC CCACTGGAGA CAGAGGACCG
    CGTGGACAAA GGGGCCCAGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCGTTGGC
    CCTCCTGGTC CCCCTGGTGC CCCTGGCCCC CCTGGTCCCC CTGGCCCCCC TGGTCTTACT
    GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAG CTGGCCCCGG ACCAATGGGC
    TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTCGGAGCCC CTGGCCCTCA AGGTTTCCAA
    GGACCTGCTG GTGAGCCTGG TGAGCCCGGA CAAACGGGTC CTGCAGGTTC CCGTGGGCCA
    GCTGGCCCTC CTGGCAAGGC CGGTGAAGAT GGTCACCCTG GAAAACCTGG AAGACCTGGC
    GAGAGAGGAG TCGTTGGGCC ACAGGGTGCT CGTGGTTTCC CTGGGACTCC TGGACTTCCT
    GGCTTCAAAG GCATTCGAGG ACACAACGGT CTGGATGGAT TGAAAGGACA GCCTGGTGCC
    CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACCCCAGG TCAAGCAGGA
    GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGT
    GGCAGTGATG GCAGTGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGTCCT
    CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCTGTTGG TAACCCTGGC
    CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GCGGGTCTTC CGGGTCTTTC TGGCCCCGTT
    GGACCTCCTG GCAACCCTGG AGCAAATGGC CTCACTGGTG CCAAGGGAGC CACTGGACTC
    CCTGGTGTTG CTGGGGCTCC TGGTCTCCCC GGTCCCCGTG GTATTCCTGG CCCCGTTGGT
    GCTGCTGGTG CTACTGGTCC CAGAGGACTT GTTGGTGAAC CTGGTCCTGC TGGCTCCAAA
    GGAGAGACTG GTAACAAGGG TGAGCCTGGC TCTGCTGGAG CCCAAGGTCC TCCTGGTCCC
    AGCGGTGAAG AAGGAAAGAG AGGGTCCCCT GGAGAACCTG GATCTGCTGG TCCTGCAGGG
    CCTCCAGGGC TGAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT
    GGTGTGATGG GCCCTCCTGG CAATCGTGGT TCAACTGGCC CTGCTGGAGT CCGAGGTCCT
    AATGGAGATG CCGGTCGCCC TGGAGAACCT GGTCTCATGG GACCCAGAGG TCTTCCCGGG
    TCTCCTGGAA ATGTTGGCCC AGCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATCGAT
    GGCAGACCTG GCCCAATTGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC
    CCTGGACCCA AAGGCCCCTC TGGTGATCCT GGCAAACCTG GTGAGAAAGG CCATCCCGGT
    CTCGCTGGTG CTCGGGGAGC TCCAGGTCCC GACGGCAACA ATGGTGCTCA GGGCCCTCCT
    GGACCTCAGG GTGTTCAAGG TGGCAAAGGT GAACAGGGCC CTGCTGGTCC TCCAGGCTTC
    CAGGGTCTCC CGGGTCCCTC AGGTACCGCT GGAGAAGTTG GCAAGCCAGG AGAAAGGGGT
    CTTCCCGGTG AATTCGGTCT CCCTGGTCCT GCTGGTCCAA GAGGAGAACG TGGTCCCCCA
    GGTGAGAGTG GAGCTGCTGG TCCTTCTGGT CCTATTGGAA GCCGAGGTCC CTCTGGAGCC
    CCAGGGCCTG ATGGGAACAA GGGTGAAGCT GGTGCAGTCG GTGCTCCAGG CTCTGCTGGT
    GCCTCTGGTC CTGGTGGGCT TCCAGGAGAG AGGGGTGCTG CTGGCATACC TGGAGGCAAA
    GGAGAAAAGG GTGAAACTGG TCTCAGAGGT GAAATCGGCA ACCCTGGTAG AGATGGTGCT
    CGAGGTGCTC CTGGTGCTAT AGGTGCCCCT GGTCCCGCTG GAGCCTCAGG TGACCGGGGC
    GAAGCTGGTG CTGCTGGTCC TTCTGGCCCT GCTGGTCCTC GAGGTAGCCC TGGTGAACGT
    GGTGAGGTTG GTCCTGCTGG CCCCAATGGA TTTGCTGGTC CTGCTGGTTC TGCTGGCCAA
    CCTGGTGCTA AAGGAGAAAA GGGAACCAAA GGGCCTAAGG GAGAAAATGG CATCGTTGGT
    CCAACCGGAC CTGTCGGAGC TGCTGGCCCA TCTGGTCCTA ATGGCCCACC TGGCCCTGCT
    GGAAGTCGTG GTGATGGAGG GCCCCCTGGT ATGACTGGCT TCCCTGGTGC TGCTGGACGG
    ACTGGTCCCC CCGGACCCAG TGGTATTACT GGCCCCCCTG GTCCCCCTGG TGCTGCTGGT
    AAAGAAGGTA TTCGTGGTCC CCGTGGTGAC CAAGGTCCAG TTGGCCGAAC TGGAGAAATA
    GGTGCATCTG GTCCCCCTGG ATTTGCTGGC GAGAAGGGTC CTTCTGGAGA ACCTGGTACC
    ACAGGACCCC CTGGCACTGC AGGTCCTCAG GGTCTTCTTG GTGCTCCCGG TATTCTGGGT
    CTCCCTGGCT CTCGAGGTGA ACGTGGCCTG CCAGGTATTG CTGGTGCTCT GGGTGAACCT
    GGTCCTCTGG GCATTGCAGG CCCTCCTGGG GCCCGTGGTC CCCCTGGTGC TGTGGGCAGT
    CCTGGAGTTA ATGGTGCCCC TGGTGAAGCC GGTCGTGATG GTAACCCTGG CAGTGATGGT
    CCCCCAGGTC GTGATGGTCA ACCCGGACAC AAGGGAGAGC GTGGTTACCC TGGCAACATT
    GGTCCCACTG GTGCTGCAGG TGCTCCTGGT CCTCATGGTT CTGTGGGTCC TGCTGGGAAA
    CATGGAAACC GTGGTGAGCC TGGTCCTGCT GGTTCTGTTG GTCCCGTTGG TGCTGTCGGT
    CCAAGAGGTC CTAGTGGTCC TCAAGGTATT CGAGGTGACA AGGGAGAGCC TGGTGACAAA
    GGGGCCAGAG GTCTTCCTGG CTTAAAAGGA CACAATGGAT TACAGGGTCT TCCTGGTCTT
    GCTGGGCTGC ATGGTGACCA AGGAGCTCCT GGTCCCGTGG GTCCTGCTGG TCCCAGGGGT
    CCTGCTGGTC CTTCTGGCCC CATTGGCAAA GATGGTAGAT CTGGACATCC TGGACCAGTC
    GGTCCTGCTG GCGTTCGTGG CTCTCAGGGT AGCCAAGGTC CCGCTGGTCC CCCTGGTCCC
    CCTGGGCCTC CTGGGCCTCC TGGTGTCAGC GGAGGAGGCT ATGACTTTGG TTTCGAAGGA
    GACTTCTACA GGGCTGACCA GCCTCGCTCA CAGCCTTCAC TCAGACCCAA GGACTATGAA
    GTTGATGCAA CTCTGAAATC TCTCAATAAC CAAATCGAGA CCCTTCTCAC TCCTGAAGGC
    TCTAGAAAGA ACCCTGCCCG CACATGCCGT GACTTAAGAC TCAGCCACCC AGAGTGGAAG
    AGCGATTACT ACTGGATTGA CCCTAACCAA GGATGCACTA TGGATGCCAT CAAAGTGTAC
    TGCGATTTCT CTACTGGTGA AACCTGCATC CAGGCCCAAC CTGTCAACAC CCCAGCCAAG
    AATGCATACA GCCGTGCCCA GGCCAACAAG CATGTCTGGT TAGGAGAGAC CATCAATGGT
    GGCAGCCAGT TTGAATACAA CGCAGAAGGG GTGTCCTCCA AGGAAATGGC AACTCAGCTC
    GCCTTCATGC GCCTGCTAGC CAACCGTGCT TCTCAGAACA TCACCTACCA CTGCAAGAAC
    AGCATTGCGT ACCTGGACGA GGAGACAGGC CGCCTGAATA AGGCTGTCAT TCTGCAGGGC
    TCCAACGACG TCGAACTTGT TGCTGAGGGC AACAGCAGAT TCACCTACAC TGTCCTTGTC
    GATGGCTGCT CCAAAAAGAC AAATGAATGG GACAAGACAA TCATTGAATA CAAAACGAAT
    AAGCCATCTC GCCTGCCATT CCTTGACATT GCACCTCTGG ACATCGGTGG TACTAACCAA
    GAATTCCGTG TGGAGGTTGG CCCTGTCTGT TTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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