Eif4ebp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4ebp1
Entrez Gene ID	116636
Full Name	eukaryotic translation initiation factor 4E binding protein 1
Synonyms	PHAS-I
General protein information	Preferred Names eukaryotic translation initiation factor 4E binding protein 1 Names eukaryotic translation initiation factor 4E-binding protein 1 4E-BP1 eIF4E-binding protein 1 phosphorylated heat- and acid-stable protein regulated by insulin 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.3
Summary	forms a complex with eukaryotic initiation factor-4E (eIF-4E) and is anintracellular target for insulin and growth factors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053857.2	NP_446309.1	eukaryotic translation initiation factor 4E-binding protein 1

Pathways from BioSystems

Homologies

	EIF4EBP1	XP_001091933.1
Homo sapiens (human)	EIF4EBP1	NP_004086.1
Canis lupus familiaris (dog)	EIF4EBP1	XP_549448.2
Rattus norvegicus (Norway rat)	Eif4ebp1	NP_446309.1
Danio rerio (zebrafish)	eif4ebp1	NP_955939.1
Pan troglodytes (chimpanzee)	EIF4EBP1	XP_003951293.1
Bos taurus (cattle)	EIF4EBP1	NP_001071361.1
Mus musculus (house mouse)	Eif4ebp1	NP_031944.3
Gallus gallus (chicken)	EIF4EBP1	XP_424384.1

Gene Ontology
Bibliography

Related articles in PubMed

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells.
Duan P, Hu C, Quan C, Yu T, Huang W, Chen W, Tang S, Shi Y, Martin FL, Yang K
Toxicology letters26721-31(2017 Feb)

Cell type-specific control of protein synthesis and proliferation by FGF-dependent signaling to the translation repressor 4E-BP.
Ruoff R, Katsara O, Kolupaeva V
Proceedings of the National Academy of Sciences of the United States of America113(27)7545-50(2016 07)

mTOR/P70S6K promotes spermatogonia proliferation and spermatogenesis in Sprague Dawley rats.
Xu H, Shen L, Chen X, Ding Y, He J, Zhu J, Wang Y, Liu X
Reproductive biomedicine online32(2)207-17(2016 Feb)

Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, leading to neuronal apoptosis.
Zhou Q, Liu C, Liu W, Zhang H, Zhang R, Liu J, Zhang J, Xu C, Liu L, Huang S, Chen L
Toxicological sciences : an official journal of the Society of Toxicology143(1)81-96(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that 4-nonylphenol stimulates Sertoli cell autophagy and inhibits mTOR-p70S6K/4EBP1 activity through AMPK activation.
Title: 4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells.

FGF signaling differentially impacts protein synthesis through either stimulation or repression, in a cell-type-dependent manner, with 4E-BP1 being a key player.
Title: Cell type-specific control of protein synthesis and proliferation by FGF-dependent signaling to the translation repressor 4E-BP.

mTOR plays an important role in spermatogenesis by regulating p70s6k activation; 4e-bp1 is either directly or indirectly regulated by PI3K
Title: mTOR/P70S6K promotes spermatogonia proliferation and spermatogenesis in Sprague Dawley rats.

Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, resulting in caspase-dependent and -independent apoptosis in neuronal cells.
Title: Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, leading to neuronal apoptosis.

Phosphorylation of 4EBP1 is suppressed following treatment with everolimus in combination with radiation.
Title: Everolimus sensitizes Ras-transformed cells to radiation in vitro through the autophagy pathway.

The following Eif4ebp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4ebp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13307	NM_053857.2 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 4E binding protein 1 (Eif4ebp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4ebp1 ( NM_053857.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446309.1 Rattus norvegicus eukaryotic translation initiation factor 4E binding protein 1 (Eif4ebp1), mRNA.

Price & Availability↑

CloneID	ORa13307
Clone ID Related Accession (Same CDS sequence)	NM_053857.2
Accession Version	NM_053857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 4E-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U05014.1 and AI603218.1. On Aug 2, 2012 this sequence version replaced NM_053857.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U05014.1, AW919144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCGG GCAGCAGTTG CAGCCAGACT CCCAGCCGGG CTATCCCCAC TCGCCGCGTA 
GCCCTCGGCG ACGGCGTGCA GCTCCCGCCC GGGGACTACA GCACCACCCC CGGCGGCACG 
CTCTTCAGCA CCACCCCGGG AGGAACCAGA ATCATCTATG ACCGGAAATT CCTGATGGAG 
TGTCGGAACT CGCCTGTGGC CAAAACACCC CCAAAGGACC TGCCAACCAT TCCAGGGGTC 
ACTAGCCCTA CCAGCGATGA GCCTCCCATG CAGGCCAGCC AGAGCCATCT GCACAGCAGC 
CCGGAAGATA AGCGGGCAGG TGGTGAAGAG TCACAGTTTG AGATGGACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446309.1
CDS	53..406
Translation	MSAGSSCSQTPSRAIPTRRVALGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVAKTPPKDLPTIPGVTSPTSDEPPMQASQSHLHSSPEDKRAGGEESQFEMDI

Target ORF information:

RefSeq Version	NM_053857.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 4E binding protein 1 (Eif4ebp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053857.2

ORF Insert Sequence:

ATGTCGGCGG GCAGCAGTTG CAGCCAGACT CCCAGCCGGG CTATCCCCAC TCGCCGCGTA 
GCCCTCGGCG ACGGCGTGCA GCTCCCGCCC GGGGACTACA GCACCACCCC CGGCGGCACG 
CTCTTCAGCA CCACCCCGGG AGGAACCAGA ATCATCTATG ACCGGAAATT CCTGATGGAG 
TGTCGGAACT CGCCTGTGGC CAAAACACCC CCAAAGGACC TGCCAACCAT TCCAGGGGTC 
ACTAGCCCTA CCAGCGATGA GCCTCCCATG CAGGCCAGCC AGAGCCATCT GCACAGCAGC 
CCGGAAGATA AGCGGGCAGG TGGTGAAGAG TCACAGTTTG AGATGGACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells.
Toxicology letters26721-31(2017 Feb)
Duan P,Hu C,Quan C,Yu T,Huang W,Chen W,Tang S,Shi Y,Martin FL,Yang K

Cell type-specific control of protein synthesis and proliferation by FGF-dependent signaling to the translation repressor 4E-BP.
Proceedings of the National Academy of Sciences of the United States of America113(27)7545-50(2016 07)
Ruoff R,Katsara O,Kolupaeva V

mTOR/P70S6K promotes spermatogonia proliferation and spermatogenesis in Sprague Dawley rats.
Reproductive biomedicine online32(2)207-17(2016 Feb)
Xu H,Shen L,Chen X,Ding Y,He J,Zhu J,Wang Y,Liu X

Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, leading to neuronal apoptosis.
Toxicological sciences : an official journal of the Society of Toxicology143(1)81-96(2015 Jan)
Zhou Q,Liu C,Liu W,Zhang H,Zhang R,Liu J,Zhang J,Xu C,Liu L,Huang S,Chen L

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Eif4ebp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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