Nfasc cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following Nfasc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfasc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa57977 XM_017598639.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57978 XM_017598640.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57979 XM_017598641.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57980 XM_017598642.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57981 XM_017598643.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57982 XM_017598644.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa57983 XM_017598645.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa57983 XM_017598646.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa57984 XM_017598647.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa57985 XM_017598648.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa57986 XM_017598649.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa57987 XM_017598650.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa12769 NM_001160314.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa12772 NM_001160315.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
ORa12778 NM_053909.2
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa12781 NM_001160313.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa35282 XM_006249738.3
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa35271 XM_006249727.3
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
ORa35271 XM_017598638.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
ORa35278 XM_006249734.3
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
ORa35285 XM_017598652.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
ORa35285 XM_017598651.1
Latest version!
Rattus norvegicus neurofascin (Nfasc), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa57977
    Clone ID Related Accession (Same CDS sequence) XM_017598639.1
    Accession Version XM_017598639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC
    AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG
    GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC
    ACAACAGTTC CCATCATCCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA
    ACTACTACAA CCACTGCCGC CGCCACCACC ACTACCACCA CGGAGAGTCC TCCCACTACC
    ACCACTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAAGCCCAGC CCATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGACTGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC
    ATTGCCCTTC TGGTGCTGAT CCTGCTTATC GTCTGCTTCA TCAAGAGGAG TCGTGGCGGC
    AAGTACCCAG TGCGAGAAAA GAAGGATGTC CCCTTGGGCC CTGAAGACCC CAAAGAAGAA
    GATGGTTCAT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT
    CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TAGTGGACTA CGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAGAAAGGAC
    AAGGAGGAGA CCGAGGGCAA TGAGAGCTCA GAGGCCACAT CTCCAGTCAA TGCCATCTAT
    TCCCTGGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454128.1
    CDS53..4444
    Translation

    Target ORF information:

    RefSeq Version XM_017598639.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC
    AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG
    GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC
    ACAACAGTTC CCATCATCCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA
    ACTACTACAA CCACTGCCGC CGCCACCACC ACTACCACCA CGGAGAGTCC TCCCACTACC
    ACCACTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACG
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC
    CAAGCCCAGC CCATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGACTGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC
    ATTGCCCTTC TGGTGCTGAT CCTGCTTATC GTCTGCTTCA TCAAGAGGAG TCGTGGCGGC
    AAGTACCCAG TGCGAGAAAA GAAGGATGTC CCCTTGGGCC CTGAAGACCC CAAAGAAGAA
    GATGGTTCAT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT
    CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TAGTGGACTA CGGCGAGGGT
    GGCGAGGGCC AGTTCAACGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAGAAAGGAC
    AAGGAGGAGA CCGAGGGCAA TGAGAGCTCA GAGGCCACAT CTCCAGTCAA TGCCATCTAT
    TCCCTGGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57978
    Clone ID Related Accession (Same CDS sequence) XM_017598640.1
    Accession Version XM_017598640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGGGC GGCCAGACCG ACCCAGGGAC
    CTGGAGCTCA CTGACCTGGC GGAAAGGAGT GTGAGGCTGA CCTGGATCCC GGGGGATGAT
    AACAACAGTC CCATCACAGA CTACGTTGTC CAGTTTGAAG AGGACCAGTT CCAGCCAGGA
    GTCTGGCATG ACCACTCCAA GTTCCCAGGC AGTGTCAACT CAGCCGTCCT CCATCTGTCC
    CCGTATGTCA ACTATCAGTT CCGAGTCATC GCTGTCAACG AGGTTGGGAG CAGCCACCCC
    AGCCTTCCAT CCGAGCGGTA CCGAACAAGC GGGGCACCCC CTGAATCCAA CCCCAGTGAT
    GTGAAGGGCG AAGGGACACG AAAAAACAAT ATGGAGATCA CATGGACGCC TATGAATGCT
    ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC ATTGTCAAGT GGCGACGGAG AGAAACCCGA
    GAGACTTGGA ACAATGTCAC CGTGTGGGGC TCTCGCTATG TGGTGGGGCA GACCCCTGTC
    TACGTACCCT ATGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAAGG CCCGGAGCCT
    GAAACCGTCA TTGGGTACTC GGGGGAAGAT TATCCCAGGG CTGCACCCAC TGAAGTTAAA
    ATCCGAGTCC TGAACAGCAC AGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCTGACACG
    GTCCAGGGCC AGCTCAGAGA GTATCGAGCT TACTACTGGA GGGAAAGCAG TTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGACAGCAG GCCAGCTTCC CTGGCGACCG CCCCCGGGGC
    GTGGTGGGTC GCCTGTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    AGAGGTGATG GGCCTCGAAG TGAAACCAAG GAATTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCAGAGT CCGACAGCCC AACCTGGAGA CCATCAACCT GGAATGGGAT
    CACCCAGAGC ACCCCAACGG GATCCTGATT GGATACACGC TCAGATACGT GCCCTTTAAT
    GGAACCAAAC TGGGAAAGCA GATGGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCTCG
    GTGCAGAGAG CAGACCCCGT GTCCCGTTAC CGCTTCTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC CCAACACCTC CAAATGAAGC TACTCCAACT
    GCAGCTCCTC CCACATTGCC CCCGACTACC GTGGGTACCA CAGGCCTTGT GAGCAGTACT
    GATGCTACTG CCCTTGCTGC CACCAGTGAA GCCACAACAG TTCCCATCAT CCCAACCGTC
    GTACCTACCA CCGTCGCCAC CACCATTGCC ACAACTACTA CAACCACTGC CGCCGCCACC
    ACCACTACCA CCACGGAGAG TCCTCCCACT ACCACCACTG GGACTAAGAT TCACGAAACC
    GCCCCCGACG AGCAGTCCAT TTGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA AGCACAATTT CAGGCCTGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG GCCCAAGCCC AGCCCATACA GCTGACAGAC
    CTCTTTCCCG GGATGACGTA CACGTTGCGG GTGTATTCCC GGGACAACGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454129.1
    CDS53..4417
    Translation

    Target ORF information:

    RefSeq Version XM_017598640.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGGGC GGCCAGACCG ACCCAGGGAC
    CTGGAGCTCA CTGACCTGGC GGAAAGGAGT GTGAGGCTGA CCTGGATCCC GGGGGATGAT
    AACAACAGTC CCATCACAGA CTACGTTGTC CAGTTTGAAG AGGACCAGTT CCAGCCAGGA
    GTCTGGCATG ACCACTCCAA GTTCCCAGGC AGTGTCAACT CAGCCGTCCT CCATCTGTCC
    CCGTATGTCA ACTATCAGTT CCGAGTCATC GCTGTCAACG AGGTTGGGAG CAGCCACCCC
    AGCCTTCCAT CCGAGCGGTA CCGAACAAGC GGGGCACCCC CTGAATCCAA CCCCAGTGAT
    GTGAAGGGCG AAGGGACACG AAAAAACAAT ATGGAGATCA CATGGACGCC TATGAATGCT
    ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC ATTGTCAAGT GGCGACGGAG AGAAACCCGA
    GAGACTTGGA ACAATGTCAC CGTGTGGGGC TCTCGCTATG TGGTGGGGCA GACCCCTGTC
    TACGTACCCT ATGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAAGG CCCGGAGCCT
    GAAACCGTCA TTGGGTACTC GGGGGAAGAT TATCCCAGGG CTGCACCCAC TGAAGTTAAA
    ATCCGAGTCC TGAACAGCAC AGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCTGACACG
    GTCCAGGGCC AGCTCAGAGA GTATCGAGCT TACTACTGGA GGGAAAGCAG TTTGCTGAAG
    AACCTGTGGG TGTCTCAGAA GAGACAGCAG GCCAGCTTCC CTGGCGACCG CCCCCGGGGC
    GTGGTGGGTC GCCTGTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG
    AGAGGTGATG GGCCTCGAAG TGAAACCAAG GAATTCACCA CCCCGGAAGG AGTACCCAGT
    GCCCCCAGGC GTTTCAGAGT CCGACAGCCC AACCTGGAGA CCATCAACCT GGAATGGGAT
    CACCCAGAGC ACCCCAACGG GATCCTGATT GGATACACGC TCAGATACGT GCCCTTTAAT
    GGAACCAAAC TGGGAAAGCA GATGGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCTCG
    GTGCAGAGAG CAGACCCCGT GTCCCGTTAC CGCTTCTCCC TCAGTGCCAG GACGCAGGTG
    GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC CCAACACCTC CAAATGAAGC TACTCCAACT
    GCAGCTCCTC CCACATTGCC CCCGACTACC GTGGGTACCA CAGGCCTTGT GAGCAGTACT
    GATGCTACTG CCCTTGCTGC CACCAGTGAA GCCACAACAG TTCCCATCAT CCCAACCGTC
    GTACCTACCA CCGTCGCCAC CACCATTGCC ACAACTACTA CAACCACTGC CGCCGCCACC
    ACCACTACCA CCACGGAGAG TCCTCCCACT ACCACCACTG GGACTAAGAT TCACGAAACC
    GCCCCCGACG AGCAGTCCAT TTGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC
    ATCACCTGGA AGCACAATTT CAGGCCTGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC
    AACCATACGA AAAAAACTGT CCCTGTTAAG GCCCAAGCCC AGCCCATACA GCTGACAGAC
    CTCTTTCCCG GGATGACGTA CACGTTGCGG GTGTATTCCC GGGACAACGA GGGCATCAGC
    AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57979
    Clone ID Related Accession (Same CDS sequence) XM_017598641.1
    Accession Version XM_017598641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT
    CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA
    CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG
    AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT
    GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG
    CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC
    CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC
    CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC
    TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC
    CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT
    GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT
    GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC
    CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC
    CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG
    GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC
    TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC
    TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC
    ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT
    CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG
    CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC
    AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG
    CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT
    GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT
    CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG
    GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT
    TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA
    CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC
    GTCATTGGGT ACTCGGGGGA AGATTATCCC AGGGCTGCAC CCACTGAAGT TAAAATCCGA
    GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCTGA CACGGTCCAG
    GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGCG ACCGCCCCCG GGGCGTGGTG
    GGTCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT
    GATGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAATG GGATCACCCA
    GAGCACCCCA ACGGGATCCT GATTGGATAC ACGCTCAGAT ACGTGCCCTT TAATGGAACC
    AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCGGTGCAG
    AGAGCAGACC CCGTGTCCCG TTACCGCTTC TCCCTCAGTG CCAGGACGCA GGTGGGCTCT
    GGAGAAGCAG CCACGGAGGA GTCCCCAACA CCTCCAAATG AAGCTACTCC AACTGCAGCT
    CCTCCCACAT TGCCCCCGAC TACCGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT
    ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATCCCAAC CGTCGTACCT
    ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCGC CACCACCACT
    ACCACCACGG AGAGTCCTCC CACTACCACC ACTGGGACTA AGATTCACGA AACCGCCCCC
    GACGAGCAGT CCATTTGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC
    TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT
    ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA GCCCAGCCCA TACAGCTGAC AGACCTCTTT
    CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC
    GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AGACTGACAT CGCCACCCAG
    GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT GCTTATCGTC
    TGCTTCATCA AGAGGAGTCG TGGCGGCAAG TACCCAGTGC GAGAAAAGAA GGATGTCCCC
    TTGGGCCCTG AAGACCCCAA AGAAGAAGAT GGTTCATTTG ACTACAGTGA CGAGGACAAC
    AAGCCCCTGC AGGGCAGCCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC
    GACAGCCTAG TGGACTACGG CGAGGGTGGC GAGGGCCAGT TCAACGAAGA TGGCTCCTTT
    ATTGGCCAGT ACACTGTCAG AAAGGACAAG GAGGAGACCG AGGGCAATGA GAGCTCAGAG
    GCCACATCTC CAGTCAATGC CATCTATTCC CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454130.1
    CDS52..4410
    Translation

    Target ORF information:

    RefSeq Version XM_017598641.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT
    CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA
    CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG
    AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT
    GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG
    CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC
    CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC
    CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC
    TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC
    CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT
    GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT
    GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC
    CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC
    CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG
    GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC
    TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC
    TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC
    ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT
    CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG
    CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC
    AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG
    CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT
    GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT
    CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG
    GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT
    TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA
    CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC
    GTCATTGGGT ACTCGGGGGA AGATTATCCC AGGGCTGCAC CCACTGAAGT TAAAATCCGA
    GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCTGA CACGGTCCAG
    GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG
    TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGCG ACCGCCCCCG GGGCGTGGTG
    GGTCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT
    GATGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC
    AGGCGTTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAATG GGATCACCCA
    GAGCACCCCA ACGGGATCCT GATTGGATAC ACGCTCAGAT ACGTGCCCTT TAATGGAACC
    AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCGGTGCAG
    AGAGCAGACC CCGTGTCCCG TTACCGCTTC TCCCTCAGTG CCAGGACGCA GGTGGGCTCT
    GGAGAAGCAG CCACGGAGGA GTCCCCAACA CCTCCAAATG AAGCTACTCC AACTGCAGCT
    CCTCCCACAT TGCCCCCGAC TACCGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT
    ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATCCCAAC CGTCGTACCT
    ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCGC CACCACCACT
    ACCACCACGG AGAGTCCTCC CACTACCACC ACTGGGACTA AGATTCACGA AACCGCCCCC
    GACGAGCAGT CCATTTGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC
    TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT
    ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA GCCCAGCCCA TACAGCTGAC AGACCTCTTT
    CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC
    GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AGACTGACAT CGCCACCCAG
    GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT GCTTATCGTC
    TGCTTCATCA AGAGGAGTCG TGGCGGCAAG TACCCAGTGC GAGAAAAGAA GGATGTCCCC
    TTGGGCCCTG AAGACCCCAA AGAAGAAGAT GGTTCATTTG ACTACAGTGA CGAGGACAAC
    AAGCCCCTGC AGGGCAGCCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC
    GACAGCCTAG TGGACTACGG CGAGGGTGGC GAGGGCCAGT TCAACGAAGA TGGCTCCTTT
    ATTGGCCAGT ACACTGTCAG AAAGGACAAG GAGGAGACCG AGGGCAATGA GAGCTCAGAG
    GCCACATCTC CAGTCAATGC CATCTATTCC CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57980
    Clone ID Related Accession (Same CDS sequence) XM_017598642.1
    Accession Version XM_017598642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA
    AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC
    ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG
    CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT
    GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT
    GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA
    CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC
    AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC
    TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG
    GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA
    CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC
    GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT
    GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC
    ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG
    GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC
    GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA
    GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC
    CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCGACTAA CCGTTTGGCT
    GCCCTGCCCA AAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA
    AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC
    GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA
    GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA
    ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA
    AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG
    TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG
    GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT
    CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA
    ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC
    CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC
    CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG
    GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC ATTGCCCCCG
    ACTACCGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC
    AGTGAAGCCA CAACAGTTCC CATCATCCCA ACCGTCGTAC CTACCACCGT CGCCACCACC
    ATTGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CTACCACCAC GGAGAGTCCT
    CCCACTACCA CCACTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG
    AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG
    CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AAGCCCAGCC CATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG
    TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGACTGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTGCTTATCG TCTGCTTCAT CAAGAGGAGT
    CGTGGCGGCA AGTACCCAGT GCGAGAAAAG AAGGATGTCC CCTTGGGCCC TGAAGACCCC
    AAAGAAGAAG ATGGTTCATT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT AGTGGACTAC
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAA GATGGCTCCT TTATTGGCCA GTACACTGTC
    AGAAAGGACA AGGAGGAGAC CGAGGGCAAT GAGAGCTCAG AGGCCACATC TCCAGTCAAT
    GCCATCTATT CCCTGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454131.1
    CDS52..4392
    Translation

    Target ORF information:

    RefSeq Version XM_017598642.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA
    AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC
    ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG
    CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT
    GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT
    GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA
    CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC
    AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC
    TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG
    GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA
    CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC
    GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT
    GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC
    ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG
    GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC
    GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA
    GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC
    CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCGACTAA CCGTTTGGCT
    GCCCTGCCCA AAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA
    AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC
    GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA
    GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA
    ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA
    AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG
    TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG
    GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT
    CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA
    ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC
    CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC
    CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG
    GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC ATTGCCCCCG
    ACTACCGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC
    AGTGAAGCCA CAACAGTTCC CATCATCCCA ACCGTCGTAC CTACCACCGT CGCCACCACC
    ATTGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CTACCACCAC GGAGAGTCCT
    CCCACTACCA CCACTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG
    AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG
    CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT
    GTTAAGGCCC AAGCCCAGCC CATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG
    TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGACTGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTGCTTATCG TCTGCTTCAT CAAGAGGAGT
    CGTGGCGGCA AGTACCCAGT GCGAGAAAAG AAGGATGTCC CCTTGGGCCC TGAAGACCCC
    AAAGAAGAAG ATGGTTCATT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT AGTGGACTAC
    GGCGAGGGTG GCGAGGGCCA GTTCAACGAA GATGGCTCCT TTATTGGCCA GTACACTGTC
    AGAAAGGACA AGGAGGAGAC CGAGGGCAAT GAGAGCTCAG AGGCCACATC TCCAGTCAAT
    GCCATCTATT CCCTGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57981
    Clone ID Related Accession (Same CDS sequence) XM_017598643.1
    Accession Version XM_017598643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCTTA CACCAACAAC
    CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG
    GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG
    CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT
    GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC
    GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454132.1
    CDS52..4191
    Translation

    Target ORF information:

    RefSeq Version XM_017598643.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCTTA CACCAACAAC
    CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG
    GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG
    CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT
    GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC
    GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57982
    Clone ID Related Accession (Same CDS sequence) XM_017598644.1
    Accession Version XM_017598644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTTACC CAGTGCCCCC AGGCGTTTCA GAGTCCGACA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGATCACCCA GAGCACCCCA ACGGGATCCT GATTGGATAC
    ACGCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CTCGGTGCAG AGAGCAGACC CCGTGTCCCG TTACCGCTTC
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCT GGAGAAGCAG CCACGGAGGA GTCCCCAACA
    CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACAT TGCCCCCGAC TACCGTGGGT
    ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA
    ACAGTTCCCA TCATCCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT
    ACTACAACCA CTGCCGCCGC CACCACCACT ACCACCACGG AGAGTCCTCC CACTACCACC
    ACTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACGGTG
    CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC
    TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA
    GCCCAGCCCA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT
    TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC
    ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT
    GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG
    TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT
    GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG
    GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG
    GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454133.1
    CDS51..4139
    Translation

    Target ORF information:

    RefSeq Version XM_017598644.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTTACC CAGTGCCCCC AGGCGTTTCA GAGTCCGACA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGATCACCCA GAGCACCCCA ACGGGATCCT GATTGGATAC
    ACGCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CTCGGTGCAG AGAGCAGACC CCGTGTCCCG TTACCGCTTC
    TCCCTCAGTG CCAGGACGCA GGTGGGCTCT GGAGAAGCAG CCACGGAGGA GTCCCCAACA
    CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACAT TGCCCCCGAC TACCGTGGGT
    ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA
    ACAGTTCCCA TCATCCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT
    ACTACAACCA CTGCCGCCGC CACCACCACT ACCACCACGG AGAGTCCTCC CACTACCACC
    ACTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACGGTG
    CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC
    TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA
    GCCCAGCCCA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT
    TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC
    ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT
    GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG
    TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT
    GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG
    GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG
    GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57983
    Clone ID Related Accession (Same CDS sequence) XM_017598645.1 , XM_017598646.1
    Accession Version XM_017598645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454134.1
    CDS51..3953
    Translation

    Target ORF information:

    RefSeq Version XM_017598645.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57983
    Clone ID Related Accession (Same CDS sequence) XM_017598645.1 , XM_017598646.1
    Accession Version XM_017598646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454135.1
    CDS51..3953
    Translation

    Target ORF information:

    RefSeq Version XM_017598646.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57984
    Clone ID Related Accession (Same CDS sequence) XM_017598647.1
    Accession Version XM_017598647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTTACA CCAACAACCA GACTGACATC
    GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGTGCCATTG CCCTTCTGGT GCTGATCCTG
    CTTATCGTCT GCTTCATCAA GAGGAGTCGT GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG
    GATGTCCCCT TGGGCCCTGA AGACCCCAAA GAAGAAGATG GTTCATTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG
    GAGAGTGACG ACAGCCTAGT GGACTACGGC GAGGGTGGCG AGGGCCAGTT CAACGAAGAT
    GGCTCCTTTA TTGGCCAGTA CACTGTCAGA AAGGACAAGG AGGAGACCGA GGGCAATGAG
    AGCTCAGAGG CCACATCTCC AGTCAATGCC ATCTATTCCC TGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454136.1
    CDS50..3937
    Translation

    Target ORF information:

    RefSeq Version XM_017598647.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTTACA CCAACAACCA GACTGACATC
    GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGTGCCATTG CCCTTCTGGT GCTGATCCTG
    CTTATCGTCT GCTTCATCAA GAGGAGTCGT GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG
    GATGTCCCCT TGGGCCCTGA AGACCCCAAA GAAGAAGATG GTTCATTTGA CTACAGTGAC
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG
    GAGAGTGACG ACAGCCTAGT GGACTACGGC GAGGGTGGCG AGGGCCAGTT CAACGAAGAT
    GGCTCCTTTA TTGGCCAGTA CACTGTCAGA AAGGACAAGG AGGAGACCGA GGGCAATGAG
    AGCTCAGAGG CCACATCTCC AGTCAATGCC ATCTATTCCC TGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57985
    Clone ID Related Accession (Same CDS sequence) XM_017598648.1
    Accession Version XM_017598648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC
    AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454137.1
    CDS51..3935
    Translation

    Target ORF information:

    RefSeq Version XM_017598648.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC
    AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57986
    Clone ID Related Accession (Same CDS sequence) XM_017598649.1
    Accession Version XM_017598649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG
    GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA
    GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG
    TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG
    TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA
    CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC
    ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC
    TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC
    ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA
    GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA
    AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA
    GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC
    GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG
    CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC
    GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC
    ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454138.1
    CDS50..3874
    Translation

    Target ORF information:

    RefSeq Version XM_017598649.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG
    GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA
    GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG
    TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG
    TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA
    CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC
    ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC
    TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC
    ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA
    GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA
    AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA
    GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC
    GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG
    CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC
    GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC
    ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57987
    Clone ID Related Accession (Same CDS sequence) XM_017598650.1
    Accession Version XM_017598650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA
    AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC
    ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG
    CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT
    GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT
    GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA
    CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC
    AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC
    TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG
    GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA
    CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC
    GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT
    GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC
    ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG
    GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC
    GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA
    GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC
    CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGGCCAGACC GACCCAGGGA CCTGGAGCTC
    ACTGACCTGG CGGAAAGGAG TGTGAGGCTG ACCTGGATCC CGGGGGATGA TAACAACAGT
    CCCATCACAG ACTACGTTGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG AGTCTGGCAT
    GACCACTCCA AGTTCCCAGG CAGTGTCAAC TCAGCCGTCC TCCATCTGTC CCCGTATGTC
    AACTATCAGT TCCGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA
    TCCGAGCGGT ACCGAACAAG CGGGGCACCC CCTGAATCCA ACCCCAGTGA TGTGAAGGGC
    GAAGGGACAC GAAAAAACAA TATGGAGATC ACATGGACGC CTATGAATGC TACCTCCGCC
    TTTGGCCCCA ACCTGCGCTA CATTGTCAAG TGGCGACGGA GAGAAACCCG AGAGACTTGG
    AACAATGTCA CCGTGTGGGG CTCTCGCTAT GTGGTGGGGC AGACCCCTGT CTACGTACCC
    TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCGGAGCC TGAAACCGTC
    ATTGGGTACT CGGGGGAAGA TTATCCCAGG GCTGCACCCA CTGAAGTTAA AATCCGAGTC
    CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCTGACAC GGTCCAGGGC
    CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGCGACC GCCCCCGGGG CGTGGTGGGT
    CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGTGAT
    GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA TCACCCAGAG
    CACCCCAACG GGATCCTGAT TGGATACACG CTCAGATACG TGCCCTTTAA TGGAACCAAA
    CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC GGTGCAGAGA
    GCAGACCCCG TGTCCCGTTA CCGCTTCTCC CTCAGTGCCA GGACGCAGGT GGGCTCTGGA
    GAAGCAGCCA CGGAGGAGTC CCCAACACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC
    ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT
    GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG
    TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT
    GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG
    GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG
    GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454139.1
    CDS49..3837
    Translation

    Target ORF information:

    RefSeq Version XM_017598650.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA
    AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC
    ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG
    CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT
    GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT
    GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA
    CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC
    AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC
    TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG
    GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA
    CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC
    GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT
    GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC
    ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG
    GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC
    GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA
    GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC
    CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGGCCAGACC GACCCAGGGA CCTGGAGCTC
    ACTGACCTGG CGGAAAGGAG TGTGAGGCTG ACCTGGATCC CGGGGGATGA TAACAACAGT
    CCCATCACAG ACTACGTTGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG AGTCTGGCAT
    GACCACTCCA AGTTCCCAGG CAGTGTCAAC TCAGCCGTCC TCCATCTGTC CCCGTATGTC
    AACTATCAGT TCCGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA
    TCCGAGCGGT ACCGAACAAG CGGGGCACCC CCTGAATCCA ACCCCAGTGA TGTGAAGGGC
    GAAGGGACAC GAAAAAACAA TATGGAGATC ACATGGACGC CTATGAATGC TACCTCCGCC
    TTTGGCCCCA ACCTGCGCTA CATTGTCAAG TGGCGACGGA GAGAAACCCG AGAGACTTGG
    AACAATGTCA CCGTGTGGGG CTCTCGCTAT GTGGTGGGGC AGACCCCTGT CTACGTACCC
    TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCGGAGCC TGAAACCGTC
    ATTGGGTACT CGGGGGAAGA TTATCCCAGG GCTGCACCCA CTGAAGTTAA AATCCGAGTC
    CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCTGACAC GGTCCAGGGC
    CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG
    GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGCGACC GCCCCCGGGG CGTGGTGGGT
    CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGTGAT
    GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG
    CGTTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA TCACCCAGAG
    CACCCCAACG GGATCCTGAT TGGATACACG CTCAGATACG TGCCCTTTAA TGGAACCAAA
    CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC GGTGCAGAGA
    GCAGACCCCG TGTCCCGTTA CCGCTTCTCC CTCAGTGCCA GGACGCAGGT GGGCTCTGGA
    GAAGCAGCCA CGGAGGAGTC CCCAACACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC
    ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT
    GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG
    TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT
    GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG
    GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC
    GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG
    GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC
    CTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa12769
    Clone ID Related Accession (Same CDS sequence) NM_001160314.1
    Accession Version NM_001160314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AY061639.1, U81036.1, AC105703.6 and BF410020.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCACCCG AGGAGTTGCG
    GAAAGAACAC CTAGCTTCAT GTATCCCCAG GGCACGTCAA GCAGTCAGAT GGTACTGCGC
    GGCATGGACC TGCTGCTGGA GTGCATTGCC TCTGGGGTCC CAACACCAGA TATTGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TCGAGAATTT TAACAAGGCT
    CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCCCC ATACTGGCTG
    GATGAGCCCA AGAACTTGAT CCTGGCTCCT GGTGAAGATG GGAGGCTGGT GTGTCGAGCC
    AATGGGAACC CGAAGCCGAC CGTCCAGTGG CTGGTGAATG GAGACCCTTT GCAATCGGCA
    CCACCCAACC CCAACCGTGA GGTGGCCGGA GACACTATCA TCTTCCGGGA CACTCAGATC
    AGCAGCAGGG CAGTGTACCA GTGTAACACA TCCAACGAAC ATGGCTACCT GCTGGCCAAT
    GCCTTCGTCA GCGTATTAGA TGTACCCCCT CGGATGCTGT CTCCCCGGAA CCAGCTCATC
    AGGGTGATCC TTTACAACCG GACGCGACTG GACTGTCCGT TCTTTGGGTC TCCCATTCCA
    ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAAAATG GCAGCCTGGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTGGCCA CCAACATCCT GGGAAAAGCT GAAAATCAAG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACAGGAT GCCTGAAGAC CAGGTGGCCA AGAGGGGCAC CACAGTGCAG
    CTGGAGTGCC GTGTGAAGCA TGACCCCTCC TTGAAACTCA CAGTCTCCTG GCTGAAGGAT
    GACGAGCCAC TCTACATTGG AAACAGGATG AAAAAGGAAG ATGACTCCCT GACCATCTTC
    GGAGTGGCAG AGCGGGACCA AGGCAGTTAC ACGTGCATGG CCAGCACCGA GCTGGACCAG
    GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCGAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGGCG GCCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCG
    GAAAGGAGTG TGAGGCTGAC CTGGATCCCG GGGGATGATA ACAACAGTCC CATCACAGAC
    TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGAG TCTGGCATGA CCACTCCAAG
    TTCCCAGGCA GTGTCAACTC AGCCGTCCTC CATCTGTCCC CGTATGTCAA CTATCAGTTC
    CGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC
    CGAACAAGCG GGGCACCCCC TGAATCCAAC CCCAGTGATG TGAAGGGCGA AGGGACACGA
    AAAAACAATA TGGAGATCAC ATGGACGCCT ATGAATGCTA CCTCCGCCTT TGGCCCCAAC
    CTGCGCTACA TTGTCAAGTG GCGACGGAGA GAAACCCGAG AGACTTGGAA CAATGTCACC
    GTGTGGGGCT CTCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTACCCTA TGAGATCCGA
    GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCGGAGCCTG AAACCGTCAT TGGGTACTCG
    GGGGAAGATT TACCCAGTGC CCCCAGGCGT TTCAGAGTCC GACAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGATCA CCCAGAGCAC CCCAACGGGA TCCTGATTGG ATACACGCTC
    AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCTCGGT GCAGAGAGCA GACCCCGTGT CCCGTTACCG CTTCTCCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGAGAA GCAGCCACGG AGGAGTCCCC AACACCTCCA
    AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTACCACA
    GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT
    CCCATCATCC CAACGGTGGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA
    ACCACTGCCG CCGCCACCAC CACTACCACC ACGGAGAGTC CTCCCACTAC CACCACTGGG
    ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAAGCCCAG
    CCCATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG
    GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTGAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153786.1
    CDS309..4031
    Translation

    Target ORF information:

    RefSeq Version NM_001160314.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCACCCG AGGAGTTGCG
    GAAAGAACAC CTAGCTTCAT GTATCCCCAG GGCACGTCAA GCAGTCAGAT GGTACTGCGC
    GGCATGGACC TGCTGCTGGA GTGCATTGCC TCTGGGGTCC CAACACCAGA TATTGCATGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TCGAGAATTT TAACAAGGCT
    CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCCCC ATACTGGCTG
    GATGAGCCCA AGAACTTGAT CCTGGCTCCT GGTGAAGATG GGAGGCTGGT GTGTCGAGCC
    AATGGGAACC CGAAGCCGAC CGTCCAGTGG CTGGTGAATG GAGACCCTTT GCAATCGGCA
    CCACCCAACC CCAACCGTGA GGTGGCCGGA GACACTATCA TCTTCCGGGA CACTCAGATC
    AGCAGCAGGG CAGTGTACCA GTGTAACACA TCCAACGAAC ATGGCTACCT GCTGGCCAAT
    GCCTTCGTCA GCGTATTAGA TGTACCCCCT CGGATGCTGT CTCCCCGGAA CCAGCTCATC
    AGGGTGATCC TTTACAACCG GACGCGACTG GACTGTCCGT TCTTTGGGTC TCCCATTCCA
    ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGTAA CTACCACGTC
    TACGAAAATG GCAGCCTGGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC
    TGTGTGGCCA CCAACATCCT GGGAAAAGCT GAAAATCAAG TCCGCCTGGA GGTCAAAGAC
    CCCACCAGGA TCTACAGGAT GCCTGAAGAC CAGGTGGCCA AGAGGGGCAC CACAGTGCAG
    CTGGAGTGCC GTGTGAAGCA TGACCCCTCC TTGAAACTCA CAGTCTCCTG GCTGAAGGAT
    GACGAGCCAC TCTACATTGG AAACAGGATG AAAAAGGAAG ATGACTCCCT GACCATCTTC
    GGAGTGGCAG AGCGGGACCA AGGCAGTTAC ACGTGCATGG CCAGCACCGA GCTGGACCAG
    GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCGAC TAACCGTTTG
    GCTGCCCTGC CCAAAGGGCG GCCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCG
    GAAAGGAGTG TGAGGCTGAC CTGGATCCCG GGGGATGATA ACAACAGTCC CATCACAGAC
    TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGAG TCTGGCATGA CCACTCCAAG
    TTCCCAGGCA GTGTCAACTC AGCCGTCCTC CATCTGTCCC CGTATGTCAA CTATCAGTTC
    CGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC
    CGAACAAGCG GGGCACCCCC TGAATCCAAC CCCAGTGATG TGAAGGGCGA AGGGACACGA
    AAAAACAATA TGGAGATCAC ATGGACGCCT ATGAATGCTA CCTCCGCCTT TGGCCCCAAC
    CTGCGCTACA TTGTCAAGTG GCGACGGAGA GAAACCCGAG AGACTTGGAA CAATGTCACC
    GTGTGGGGCT CTCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTACCCTA TGAGATCCGA
    GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCGGAGCCTG AAACCGTCAT TGGGTACTCG
    GGGGAAGATT TACCCAGTGC CCCCAGGCGT TTCAGAGTCC GACAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGATCA CCCAGAGCAC CCCAACGGGA TCCTGATTGG ATACACGCTC
    AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCTCGGT GCAGAGAGCA GACCCCGTGT CCCGTTACCG CTTCTCCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGAGAA GCAGCCACGG AGGAGTCCCC AACACCTCCA
    AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTACCACA
    GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT
    CCCATCATCC CAACGGTGGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA
    ACCACTGCCG CCGCCACCAC CACTACCACC ACGGAGAGTC CTCCCACTAC CACCACTGGG
    ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC GGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAAGCCCAG
    CCCATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG
    GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC
    AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT
    CTGGTGCTGA TCCTGCTGAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA
    GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA
    TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC
    CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG
    ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa12772
    Clone ID Related Accession (Same CDS sequence) NM_001160315.1
    Accession Version NM_001160315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AC105703.6 and BF410020.1. Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and lacks two segments in the N-terminal and central regions, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCACC CGAGGAGTTG CGGAAAGAAC ACCTAGCTTC
    ATGTATCCCC AGGGCACGTC AAGCAGTCAG ATGGTACTGC GCGGCATGGA CCTGCTGCTG
    GAGTGCATTG CCTCTGGGGT CCCAACACCA GATATTGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTCGAGAAT TTTAACAAGG CTCTGCGCAT CACCAATGTC
    TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCC CCATACTGGC TGGATGAGCC CAAGAACTTG
    ATCCTGGCTC CTGGTGAAGA TGGGAGGCTG GTGTGTCGAG CCAATGGGAA CCCGAAGCCG
    ACCGTCCAGT GGCTGGTGAA TGGAGACCCT TTGCAATCGG CACCACCCAA CCCCAACCGT
    GAGGTGGCCG GAGACACTAT CATCTTCCGG GACACTCAGA TCAGCAGCAG GGCAGTGTAC
    CAGTGTAACA CATCCAACGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTATTA
    GATGTACCCC CTCGGATGCT GTCTCCCCGG AACCAGCTCA TCAGGGTGAT CCTTTACAAC
    CGGACGCGAC TGGACTGTCC GTTCTTTGGG TCTCCCATTC CAACACTCCG ATGGTTTAAG
    AATGGGCAAG GAAGCAACCT GGATGGTGGT AACTACCACG TCTACGAAAA TGGCAGCCTG
    GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC
    CTGGGAAAAG CTGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG
    ATGCCTGAAG ACCAGGTGGC CAAGAGGGGC ACCACAGTGC AGCTGGAGTG CCGTGTGAAG
    CATGACCCCT CCTTGAAACT CACAGTCTCC TGGCTGAAGG ATGACGAGCC ACTCTACATT
    GGAAACAGGA TGAAAAAGGA AGATGACTCC CTGACCATCT TCGGAGTGGC AGAGCGGGAC
    CAAGGCAGTT ACACGTGCAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC
    CTCACTGTTC TAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA
    AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC
    GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA
    GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA
    ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA
    AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG
    TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG
    GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT
    CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA
    ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC
    CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC
    CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG
    GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA CAACCAGACT
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG
    ATCCTGCTTA TCGTCTGCTT CATCAAGAGG AGTCGTGGCG GCAAGTACCC AGTGCGAGAA
    AAGAAGGATG TCCCCTTGGG CCCTGAAGAC CCCAAAGAAG AAGATGGTTC ATTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGACGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTAGTGGAC TACGGCGAGG GTGGCGAGGG CCAGTTCAAC
    GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAGAAAGG ACAAGGAGGA GACCGAGGGC
    AATGAGAGCT CAGAGGCCAC ATCTCCAGTC AATGCCATCT ATTCCCTGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153787.1
    CDS518..3991
    Translation

    Target ORF information:

    RefSeq Version NM_001160315.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCACC CGAGGAGTTG CGGAAAGAAC ACCTAGCTTC
    ATGTATCCCC AGGGCACGTC AAGCAGTCAG ATGGTACTGC GCGGCATGGA CCTGCTGCTG
    GAGTGCATTG CCTCTGGGGT CCCAACACCA GATATTGCAT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTCGAGAAT TTTAACAAGG CTCTGCGCAT CACCAATGTC
    TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCC CCATACTGGC TGGATGAGCC CAAGAACTTG
    ATCCTGGCTC CTGGTGAAGA TGGGAGGCTG GTGTGTCGAG CCAATGGGAA CCCGAAGCCG
    ACCGTCCAGT GGCTGGTGAA TGGAGACCCT TTGCAATCGG CACCACCCAA CCCCAACCGT
    GAGGTGGCCG GAGACACTAT CATCTTCCGG GACACTCAGA TCAGCAGCAG GGCAGTGTAC
    CAGTGTAACA CATCCAACGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTATTA
    GATGTACCCC CTCGGATGCT GTCTCCCCGG AACCAGCTCA TCAGGGTGAT CCTTTACAAC
    CGGACGCGAC TGGACTGTCC GTTCTTTGGG TCTCCCATTC CAACACTCCG ATGGTTTAAG
    AATGGGCAAG GAAGCAACCT GGATGGTGGT AACTACCACG TCTACGAAAA TGGCAGCCTG
    GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC
    CTGGGAAAAG CTGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG
    ATGCCTGAAG ACCAGGTGGC CAAGAGGGGC ACCACAGTGC AGCTGGAGTG CCGTGTGAAG
    CATGACCCCT CCTTGAAACT CACAGTCTCC TGGCTGAAGG ATGACGAGCC ACTCTACATT
    GGAAACAGGA TGAAAAAGGA AGATGACTCC CTGACCATCT TCGGAGTGGC AGAGCGGGAC
    CAAGGCAGTT ACACGTGCAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC
    CTCACTGTTC TAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA
    AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC
    GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA
    GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA
    ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA
    AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG
    TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC
    CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG
    GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT
    CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC
    AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA
    ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC
    CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC
    CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG
    GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA CAACCAGACT
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG
    ATCCTGCTTA TCGTCTGCTT CATCAAGAGG AGTCGTGGCG GCAAGTACCC AGTGCGAGAA
    AAGAAGGATG TCCCCTTGGG CCCTGAAGAC CCCAAAGAAG AAGATGGTTC ATTTGACTAC
    AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGACGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTAGTGGAC TACGGCGAGG GTGGCGAGGG CCAGTTCAAC
    GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAGAAAGG ACAAGGAGGA GACCGAGGGC
    AATGAGAGCT CAGAGGCCAC ATCTCCAGTC AATGCCATCT ATTCCCTGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa12778
    Clone ID Related Accession (Same CDS sequence) NM_053909.2
    Accession Version NM_053909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-19
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AY061639.1, AC105703.6 and BF410020.1. On May 21, 2009 this sequence version replaced NM_053909.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY061639.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC
    CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC
    CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT
    GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT
    CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG
    TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC
    GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC
    ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC
    CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA
    CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA
    GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG
    ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA
    GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA
    GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC
    TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG
    ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT
    TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT
    CTAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACCC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTG
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_446361.1
    CDS309..3833
    Translation

    Target ORF information:

    RefSeq Version NM_053909.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC
    CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC
    CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT
    GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT
    CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG
    TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC
    GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC
    ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC
    CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA
    CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA
    GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG
    ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA
    GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA
    GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC
    TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG
    ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT
    TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT
    CTAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG
    AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT
    GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG
    GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG
    GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT
    GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT
    CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA
    AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT
    CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT
    CAGTGGAACC GCGTCTACCC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC
    TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA
    TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA
    TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC
    TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA
    ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTG
    ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT
    GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC
    TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC
    TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa12781
    Clone ID Related Accession (Same CDS sequence) NM_001160313.1
    Accession Version NM_001160313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY061639.1, U81035.1, AC105703.6 and BF410020.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U81035.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC
    CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC
    CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT
    GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT
    CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG
    TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC
    GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC
    ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC
    CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA
    CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA
    GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG
    ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA
    GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA
    GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC
    TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG
    ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT
    TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT
    CTAGCTGATC AGGCCACTCC GACTAACCGT TTGGCTGCCC TGCCCAAAGG GCGGCCAGAC
    CGACCCAGGG ACCTGGAGCT CACTGACCTG GCGGAAAGGA GTGTGAGGCT GACCTGGATC
    CCGGGGGATG ATAACAACAG TCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GAGTCTGGCA TGACCACTCC AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC
    CTCCATCTGT CCCCGTATGT CAACTATCAG TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG
    AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACAA GCGGGGCACC CCCTGAATCC
    AACCCCAGTG ATGTGAAGGG CGAAGGGACA CGAAAAAACA ATATGGAGAT CACATGGACG
    CCTATGAATG CTACCTCCGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGCGACGG
    AGAGAAACCC GAGAGACTTG GAACAATGTC ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG
    CAGACCCCTG TCTACGTACC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA
    GGCCCGGAGC CTGAAACCGT CATTGGGTAC TCGGGGGAAG ATTATCCCAG GGCTGCACCC
    ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC
    TACCCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC
    AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGCGAC
    CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT
    GTGGTCAATG GGAGAGGTGA TGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG GCGTTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC
    CTGGAATGGG ATCACCCAGA GCACCCCAAC GGGATCCTGA TTGGATACAC GCTCAGATAC
    GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCT CGGTGCAGAG AGCAGACCCC GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG AGAAGCAGCC ACGGAGGAGT CCCCAACACC TCCAAATGAA
    GCTACTCCAA CTGCAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTGATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153785.1
    CDS91..3660
    Translation

    Target ORF information:

    RefSeq Version NM_001160313.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA
    GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG
    GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT
    GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA
    GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT
    GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC
    AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG
    AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC
    CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC
    CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT
    GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG
    GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT
    CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG
    TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC
    GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC
    ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC
    CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA
    CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA
    GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG
    ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA
    GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA
    GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC
    TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG
    ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT
    TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT
    CTAGCTGATC AGGCCACTCC GACTAACCGT TTGGCTGCCC TGCCCAAAGG GCGGCCAGAC
    CGACCCAGGG ACCTGGAGCT CACTGACCTG GCGGAAAGGA GTGTGAGGCT GACCTGGATC
    CCGGGGGATG ATAACAACAG TCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG
    TTCCAGCCAG GAGTCTGGCA TGACCACTCC AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC
    CTCCATCTGT CCCCGTATGT CAACTATCAG TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG
    AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACAA GCGGGGCACC CCCTGAATCC
    AACCCCAGTG ATGTGAAGGG CGAAGGGACA CGAAAAAACA ATATGGAGAT CACATGGACG
    CCTATGAATG CTACCTCCGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGCGACGG
    AGAGAAACCC GAGAGACTTG GAACAATGTC ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG
    CAGACCCCTG TCTACGTACC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA
    GGCCCGGAGC CTGAAACCGT CATTGGGTAC TCGGGGGAAG ATTATCCCAG GGCTGCACCC
    ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC
    TACCCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC
    AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGCGAC
    CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT
    GTGGTCAATG GGAGAGGTGA TGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCGGAA
    GGAGTACCCA GTGCCCCCAG GCGTTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC
    CTGGAATGGG ATCACCCAGA GCACCCCAAC GGGATCCTGA TTGGATACAC GCTCAGATAC
    GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG
    ACCAAGTTCT CGGTGCAGAG AGCAGACCCC GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC
    AGGACGCAGG TGGGCTCTGG AGAAGCAGCC ACGGAGGAGT CCCCAACACC TCCAAATGAA
    GCTACTCCAA CTGCAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTGATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35282
    Clone ID Related Accession (Same CDS sequence) XM_006249738.3
    Accession Version XM_006249738.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG
    GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA
    GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG
    TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG
    TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA
    CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC
    ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC
    TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC
    ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA
    GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA
    AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA
    GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC
    GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG
    CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC
    GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC
    ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAACAAC
    CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG
    GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG
    CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT
    GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC
    GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006249800.1
    CDS51..3890
    Translation

    Target ORF information:

    RefSeq Version XM_006249738.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006249738.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA
    ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT
    GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC
    TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC
    GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG
    AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG
    TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG
    TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG
    CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC
    GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC
    ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC
    TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT
    AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG
    CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC
    AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG
    AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG
    AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC
    AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA
    GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC
    GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT
    TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG
    TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC
    AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC
    AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC
    TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT
    GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC
    TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG
    CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG
    GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG
    GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA
    GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG
    TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG
    TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA
    CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC
    ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC
    CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC
    TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC
    ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA
    GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC
    CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA
    AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA
    GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC
    GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG
    CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC
    GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC
    ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAACAAC
    CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG
    GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG
    CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT
    GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG
    TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC
    GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35271
    Clone ID Related Accession (Same CDS sequence) XM_017598638.1 , XM_006249727.3
    Accession Version XM_017598638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG
    TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG
    ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454127.1
    CDS53..4462
    Translation

    Target ORF information:

    RefSeq Version XM_017598638.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG
    TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG
    ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35271
    Clone ID Related Accession (Same CDS sequence) XM_017598638.1 , XM_006249727.3
    Accession Version XM_006249727.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG
    TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG
    ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006249789.1
    CDS53..4462
    Translation

    Target ORF information:

    RefSeq Version XM_006249727.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006249727.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA
    GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA
    CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT
    GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC
    AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC
    TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT
    CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA
    TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT
    CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG
    GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG
    CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC
    ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC
    CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC
    TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC
    AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA
    GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG
    AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC
    ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG
    GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC
    CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC
    CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC
    ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT
    CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG
    CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG
    ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT
    GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG
    ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG
    TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC
    AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC
    AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG
    TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT
    CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC
    AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC
    AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA
    AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC
    CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA
    GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA
    TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT
    GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC
    GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG
    GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG
    TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG
    ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC
    AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT
    GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA
    GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG
    TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT
    CCAGTCAATG CCATCTATTC CCTGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35278
    Clone ID Related Accession (Same CDS sequence) XM_006249734.3
    Accession Version XM_006249734.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT
    CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA
    CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG
    AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT
    GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG
    CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC
    CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC
    CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC
    TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC
    CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT
    GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT
    GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC
    CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC
    CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG
    GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC
    TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC
    TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC
    ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT
    CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG
    CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC
    AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG
    CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT
    GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT
    CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG
    GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT
    TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA
    CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC
    GTCATTGGGT ACTCGGGGGA AGATTTACCC AGTGCCCCCA GGCGTTTCAG AGTCCGACAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GATCACCCAG AGCACCCCAA CGGGATCCTG
    ATTGGATACA CGCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC TCGGTGCAGA GAGCAGACCC CGTGTCCCGT
    TACCGCTTCT CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GAGAAGCAGC CACGGAGGAG
    TCCCCAACAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACATT GCCCCCGACT
    ACCGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT
    GAAGCCACAA CAGTTCCCAT CATCCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT
    GCCACAACTA CTACAACCAC TGCCGCCGCC ACCACCACTA CCACCACGGA GAGTCCTCCC
    ACTACCACCA CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT
    GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAAG CCCAGCCCAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG
    CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAACAACCA GACTGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG
    TGTGCCATTG CCCTTCTGGT GCTGATCCTG CTTATCGTCT GCTTCATCAA GAGGAGTCGT
    GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG GATGTCCCCT TGGGCCCTGA AGACCCCAAA
    GAAGAAGATG GTTCATTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTAGT GGACTACGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAGA
    AAGGACAAGG AGGAGACCGA GGGCAATGAG AGCTCAGAGG CCACATCTCC AGTCAATGCC
    ATCTATTCCC TGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006249796.1
    CDS49..4086
    Translation

    Target ORF information:

    RefSeq Version XM_006249734.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006249734.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
    GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA
    TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA
    GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG
    AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG
    GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC
    CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT
    GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC
    CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG
    ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG
    TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG
    TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG
    GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT
    GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG
    ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG
    GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA
    CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC
    ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT
    CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA
    CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG
    AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC
    TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT
    GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG
    CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC
    CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC
    CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC
    TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC
    CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT
    GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT
    GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC
    CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC
    CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG
    GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC
    TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC
    TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC
    ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT
    CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG
    CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC
    AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG
    CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT
    GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT
    CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG
    GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT
    TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA
    CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC
    GTCATTGGGT ACTCGGGGGA AGATTTACCC AGTGCCCCCA GGCGTTTCAG AGTCCGACAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GATCACCCAG AGCACCCCAA CGGGATCCTG
    ATTGGATACA CGCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC TCGGTGCAGA GAGCAGACCC CGTGTCCCGT
    TACCGCTTCT CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GAGAAGCAGC CACGGAGGAG
    TCCCCAACAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACATT GCCCCCGACT
    ACCGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT
    GAAGCCACAA CAGTTCCCAT CATCCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT
    GCCACAACTA CTACAACCAC TGCCGCCGCC ACCACCACTA CCACCACGGA GAGTCCTCCC
    ACTACCACCA CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC
    GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT
    GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT
    AAGGCCCAAG CCCAGCCCAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG
    CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT
    ACAGCTTACA CCAACAACCA GACTGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG
    TGTGCCATTG CCCTTCTGGT GCTGATCCTG CTTATCGTCT GCTTCATCAA GAGGAGTCGT
    GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG GATGTCCCCT TGGGCCCTGA AGACCCCAAA
    GAAGAAGATG GTTCATTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTAGT GGACTACGGC
    GAGGGTGGCG AGGGCCAGTT CAACGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAGA
    AAGGACAAGG AGGAGACCGA GGGCAATGAG AGCTCAGAGG CCACATCTCC AGTCAATGCC
    ATCTATTCCC TGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35285
    Clone ID Related Accession (Same CDS sequence) XM_017598651.1 , XM_017598652.1
    Accession Version XM_017598651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA
    CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC
    CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC
    ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC
    AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC
    CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC
    CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG
    GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC
    AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC
    CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA
    TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT
    GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC
    ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC
    CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA
    CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA
    GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA
    AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT
    GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC
    GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC
    GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT
    ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC
    TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT
    GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT
    TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT
    TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG
    GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT
    GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC
    CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC
    CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC
    GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA
    GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC
    ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT
    ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC
    ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA
    ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG
    GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG
    GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA
    GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT
    GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC
    GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA
    GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA
    TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG
    GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG
    GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC
    TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454140.1
    CDS291..4385
    Translation

    Target ORF information:

    RefSeq Version XM_017598651.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA
    CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC
    CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC
    ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC
    AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC
    CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC
    CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG
    GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC
    AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC
    CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA
    TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT
    GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC
    ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC
    CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA
    CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA
    GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA
    AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT
    GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC
    GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC
    GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT
    ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC
    TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT
    GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT
    TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT
    TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG
    GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT
    GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC
    CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC
    CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC
    GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA
    GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC
    ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT
    ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC
    ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA
    ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG
    GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG
    GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA
    GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT
    GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC
    GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA
    GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA
    TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG
    GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG
    GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC
    TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35285
    Clone ID Related Accession (Same CDS sequence) XM_017598651.1 , XM_017598652.1
    Accession Version XM_017598652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA
    CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC
    CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC
    ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC
    AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC
    CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC
    CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG
    GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC
    AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC
    CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA
    TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT
    GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC
    ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC
    CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA
    CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA
    GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA
    AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT
    GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC
    GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC
    GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT
    ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC
    TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT
    GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT
    TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT
    TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG
    GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT
    GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC
    CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC
    CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC
    GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA
    GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC
    ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT
    ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC
    ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA
    ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG
    GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG
    GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA
    GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT
    GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC
    GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA
    GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA
    TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG
    GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG
    GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC
    TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454141.1
    CDS287..4381
    Translation

    Target ORF information:

    RefSeq Version XM_017598652.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus neurofascin (Nfasc), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
    CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC
    CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG
    ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG
    ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC
    CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG
    CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG
    GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC
    CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA
    CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC
    CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC
    ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC
    AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC
    CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC
    CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG
    GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC
    AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC
    CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA
    TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT
    GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC
    ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG
    ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC
    CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA
    CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA
    GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA
    AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG
    CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT
    GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC
    GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC
    GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT
    ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC
    TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT
    GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT
    TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT
    TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG
    GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT
    GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC
    CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC
    CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC
    GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA
    GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC
    ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT
    ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC
    ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA
    ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG
    GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG
    GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA
    GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT
    GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC
    GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA
    GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA
    TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG
    GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG
    GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC
    TATTCCCTGG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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