Plscr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Plscr1
Entrez Gene ID	117540
Full Name	phospholipid scramblase 1
General protein information	Preferred Names phospholipid scramblase 1 Names phospholipid scramblase 1 PL scramblase 1 ca(2+)-dependent phospholipid scramblase 1 phospholipid scramblase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	may act as a downstream mediator of immune response in IgE receptor signaling; may transport phospholipids in the plasma membrane [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243536.2	XP_006243598.1	phospholipid scramblase 1 isoform X1
XM_006243537.2	XP_006243599.1	phospholipid scramblase 1 isoform X1
NM_057194.1	NP_476542.1	phospholipid scramblase 1
XM_006243538.3	XP_006243600.1	phospholipid scramblase 1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	plscr3a	NP_001098583.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005498	XP_001688666.1
Homo sapiens (human)	PLSCR1	NP_066928.1
Rattus norvegicus (Norway rat)	Plscr1	NP_476542.1
Drosophila melanogaster (fruit fly)	scramb1	NP_648389.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005504	XP_001688668.1
Caenorhabditis elegans (roundworm)	scrm-3	NP_503934.2
Xenopus tropicalis (tropical clawed frog)	LOC100489273	XP_002938452.2
Pan troglodytes (chimpanzee)	PLSCR1	XP_001135229.2
	PLSCR1	XP_001111222.1
Mus musculus (house mouse)	Plscr1	NP_035766.2
Gallus gallus (chicken)	PLSCR1	XP_001231237.1
Danio rerio (zebrafish)	plscr3b	NP_998031.1
Caenorhabditis elegans (roundworm)	scrm-2	NP_493321.1
Xenopus tropicalis (tropical clawed frog)	plscr1	NP_001135607.1
Canis lupus familiaris (dog)	LOC611500	XP_005634707.1
Caenorhabditis elegans (roundworm)	scrm-1	NP_492976.2

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
Kassas A, Moura IC, Yamashita Y, Scheffel J, Gu?rin-Marchand C, Blank U, Sims PJ, Wiedmer T, Monteiro RC, Rivera J, Charles N, Benhamou M
PloS one9(10)e109800(2014)

Phospholipid scramblase 1 functionally interacts with angiogenin and regulates angiogenin-enhanced rRNA transcription.
Zhu J, Sheng J, Dong H, Kang L, Ang J, Xu Z
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology32(6)1695-706(2013)

Identification of PLSCR1 as a protein that interacts with RELT family members.
Cusick JK, Mustian A, Jacobs AT, Reyland ME
Molecular and cellular biochemistry362(1-2)55-63(2012 Mar)

Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.
Merregaert J, Van Langen J, Hansen U, Ponsaerts P, El Ghalbzouri A, Steenackers E, Van Ostade X, Sercu S
The Journal of biological chemistry285(48)37823-37(2010 Nov)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reveals a complex regulation for PLSCR1 tyrosine phosphorylation in FcepsilonRI-activated mast cells, and that PLSCR1 sits at a crossroads between Lyn and Fyn pathways.
Title: Regulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.

PLSCR1 is a novel amplifier of FcepsilonRI signaling that acts selectively on the Lyn-initiated LAT/phospholipase Cgamma1/calcium axis, resulting in potentiation of a selected set of mast cell responses
Title: Phospholipid scramblase 1 modulates a selected set of IgE receptor-mediated mast cell responses through LAT-dependent pathway.

changes in the topography of PLSCR1 and its tyrosine phosphorylation, PS externalization, and exocytosis are independent phenomena that could be distinguished by employing specific conditions of activation
Title: Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis.

The following Plscr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plscr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11560	NM_057194.1 Latest version!	Rattus norvegicus phospholipid scramblase 1 (Plscr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa11560	XM_006243538.3 Latest version!	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa31457	XM_006243537.2 Latest version!	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa31457	XM_006243536.2 Latest version!	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plscr1 ( NM_057194.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476542.1 Rattus norvegicus phospholipid scramblase 1 (Plscr1), mRNA.

Price & Availability↑

CloneID	ORa11560
Clone ID Related Accession (Same CDS sequence)	NM_057194.1 , XM_006243538.3
Accession Version	NM_057194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid scramblase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY024347.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY024347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGC ACGGACCACC AGAACATGCT GCTTACCCCA TTCCTCAGGC TGACTACCAA 
GGGTCCCAGG GTCCCTATCC AGGACCGCAG GGTCCATATC CAGGGCCCCA GGGTCCCTAT 
GCAGGGCCTC AAGGTCCCTA TCCAGGGCCC CAGGGTCCCT ATGCAGGGCC CCAAGGTCCC 
TATCCAGGGC CCCAACCTGG CTACCCAGTT CCACCAGGCA GTTATGCAGG TGGTGACCCA 
AGTGGCTTTC CTGTCCAACA TCAGCCGGCG TATAATCACC CAGGTGGACC TGGGGGCACC 
CCATGGATGC AAGCACCACC TCCACCCCTG GACTGTCCGC CTGGGCTGGA ATACTTAACG 
CAGATAGATC AGATTCTGGT TCATCAGCAG ATTGAACTTC TGGAAGTCTT AACAGGCTTT 
GAAACAAATA ACAAATATGA AATCAAGAAC AGCCTTGGGC AGAGGGTTTA CTTTGCGGTG 
GAAGATACGG ACTGCTGCAC TCGAAACTGC TGTGGGGCGT CTAGACCGTT CACCTTGAGG 
ATCCTGGATA ACATGGGCCG AGAAGTCATG ACTCTGGAGA GACCCCTGAG ATGCAGTAGC 
TGCTGCTTCC CCTGCTGCCT CCAGGAGATA GAAATCCAGG CTCCTCCTGG GGTGCCAGTA 
GGTTATGTGA TTCAGACCTG GCACCCATGT CTGCCAAAGT TCACTCTCCA AAATGAGAAG 
AGGCAGGACG TTCTAAAGGT CGTTGGTCCG TGTGTTGTGT GTAGCTGCTG TTCAGACATT 
GACTTTGAGC TCAAATCTCT AGATGAGGAA TCCGTTGTTG GCAAAATTTC CAAGCAGTGG 
TCTGGTTTTG TGAGAGAGGC CTTCACGGAT GCAGATAACT TCGGGATCCA GTTCCCGCTG 
GACCTGGATG TGAAGATGAA AGCTGTGATG CTTGGCGCTT GTTTCCTCAT AGATTTCATG 
TTTTTTGAAA GAACTGGAAA TGAGGAACAA AGGTCAGGAG TATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476542.1
CDS	158..1165
Translation	MEKHGPPEHAAYPIPQADYQGSQGPYPGPQGPYPGPQGPYAGPQGPYPGPQGPYAGPQGPYPGPQPGYPVPPGSYAGGDPSGFPVQHQPAYNHPGGPGGTPWMQAPPPPLDCPPGLEYLTQIDQILVHQQIELLEVLTGFETNNKYEIKNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNMGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGVPVGYVIQTWHPCLPKFTLQNEKRQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW

Target ORF information:

RefSeq Version	NM_057194.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipid scramblase 1 (Plscr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057194.1

ORF Insert Sequence:

ATGGAGAAGC ACGGACCACC AGAACATGCT GCTTACCCCA TTCCTCAGGC TGACTACCAA 
GGGTCCCAGG GTCCCTATCC AGGACCGCAG GGTCCATATC CAGGGCCCCA GGGTCCCTAT 
GCAGGGCCTC AAGGTCCCTA TCCAGGGCCC CAGGGTCCCT ATGCAGGGCC CCAAGGTCCC 
TATCCAGGGC CCCAACCTGG CTACCCAGTT CCACCAGGCA GTTATGCAGG TGGTGACCCA 
AGTGGCTTTC CTGTCCAACA TCAGCCGGCG TATAATCACC CAGGTGGACC TGGGGGCACC 
CCATGGATGC AAGCACCACC TCCACCCCTG GACTGTCCGC CTGGGCTGGA ATACTTAACG 
CAGATAGATC AGATTCTGGT TCATCAGCAG ATTGAACTTC TGGAAGTCTT AACAGGCTTT 
GAAACAAATA ACAAATATGA AATCAAGAAC AGCCTTGGGC AGAGGGTTTA CTTTGCGGTG 
GAAGATACGG ACTGCTGCAC TCGAAACTGC TGTGGGGCGT CTAGACCGTT CACCTTGAGG 
ATCCTGGATA ACATGGGCCG AGAAGTCATG ACTCTGGAGA GACCCCTGAG ATGCAGTAGC 
TGCTGCTTCC CCTGCTGCCT CCAGGAGATA GAAATCCAGG CTCCTCCTGG GGTGCCAGTA 
GGTTATGTGA TTCAGACCTG GCACCCATGT CTGCCAAAGT TCACTCTCCA AAATGAGAAG 
AGGCAGGACG TTCTAAAGGT CGTTGGTCCG TGTGTTGTGT GTAGCTGCTG TTCAGACATT 
GACTTTGAGC TCAAATCTCT AGATGAGGAA TCCGTTGTTG GCAAAATTTC CAAGCAGTGG 
TCTGGTTTTG TGAGAGAGGC CTTCACGGAT GCAGATAACT TCGGGATCCA GTTCCCGCTG 
GACCTGGATG TGAAGATGAA AGCTGTGATG CTTGGCGCTT GTTTCCTCAT AGATTTCATG 
TTTTTTGAAA GAACTGGAAA TGAGGAACAA AGGTCAGGAG TATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plscr1 ( XM_006243538.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243600.1 Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa11560
Clone ID Related Accession (Same CDS sequence)	NM_057194.1 , XM_006243538.3
Accession Version	XM_006243538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid scramblase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC ACGGACCACC AGAACATGCT GCTTACCCCA TTCCTCAGGC TGACTACCAA 
GGGTCCCAGG GTCCCTATCC AGGACCGCAG GGTCCATATC CAGGGCCCCA GGGTCCCTAT 
GCAGGGCCTC AAGGTCCCTA TCCAGGGCCC CAGGGTCCCT ATGCAGGGCC CCAAGGTCCC 
TATCCAGGGC CCCAACCTGG CTACCCAGTT CCACCAGGCA GTTATGCAGG TGGTGACCCA 
AGTGGCTTTC CTGTCCAACA TCAGCCGGCG TATAATCACC CAGGTGGACC TGGGGGCACC 
CCATGGATGC AAGCACCACC TCCACCCCTG GACTGTCCGC CTGGGCTGGA ATACTTAACG 
CAGATAGATC AGATTCTGGT TCATCAGCAG ATTGAACTTC TGGAAGTCTT AACAGGCTTT 
GAAACAAATA ACAAATATGA AATCAAGAAC AGCCTTGGGC AGAGGGTTTA CTTTGCGGTG 
GAAGATACGG ACTGCTGCAC TCGAAACTGC TGTGGGGCGT CTAGACCGTT CACCTTGAGG 
ATCCTGGATA ACATGGGCCG AGAAGTCATG ACTCTGGAGA GACCCCTGAG ATGCAGTAGC 
TGCTGCTTCC CCTGCTGCCT CCAGGAGATA GAAATCCAGG CTCCTCCTGG GGTGCCAGTA 
GGTTATGTGA TTCAGACCTG GCACCCATGT CTGCCAAAGT TCACTCTCCA AAATGAGAAG 
AGGCAGGACG TTCTAAAGGT CGTTGGTCCG TGTGTTGTGT GTAGCTGCTG TTCAGACATT 
GACTTTGAGC TCAAATCTCT AGATGAGGAA TCCGTTGTTG GCAAAATTTC CAAGCAGTGG 
TCTGGTTTTG TGAGAGAGGC CTTCACGGAT GCAGATAACT TCGGGATCCA GTTCCCGCTG 
GACCTGGATG TGAAGATGAA AGCTGTGATG CTTGGCGCTT GTTTCCTCAT AGATTTCATG 
TTTTTTGAAA GAACTGGAAA TGAGGAACAA AGGTCAGGAG TATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243600.1
CDS	184..1191
Translation	MEKHGPPEHAAYPIPQADYQGSQGPYPGPQGPYPGPQGPYAGPQGPYPGPQGPYAGPQGPYPGPQPGYPVPPGSYAGGDPSGFPVQHQPAYNHPGGPGGTPWMQAPPPPLDCPPGLEYLTQIDQILVHQQIELLEVLTGFETNNKYEIKNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNMGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGVPVGYVIQTWHPCLPKFTLQNEKRQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW

Target ORF information:

RefSeq Version	XM_006243538.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243538.3

ORF Insert Sequence:

ATGGAGAAGC ACGGACCACC AGAACATGCT GCTTACCCCA TTCCTCAGGC TGACTACCAA 
GGGTCCCAGG GTCCCTATCC AGGACCGCAG GGTCCATATC CAGGGCCCCA GGGTCCCTAT 
GCAGGGCCTC AAGGTCCCTA TCCAGGGCCC CAGGGTCCCT ATGCAGGGCC CCAAGGTCCC 
TATCCAGGGC CCCAACCTGG CTACCCAGTT CCACCAGGCA GTTATGCAGG TGGTGACCCA 
AGTGGCTTTC CTGTCCAACA TCAGCCGGCG TATAATCACC CAGGTGGACC TGGGGGCACC 
CCATGGATGC AAGCACCACC TCCACCCCTG GACTGTCCGC CTGGGCTGGA ATACTTAACG 
CAGATAGATC AGATTCTGGT TCATCAGCAG ATTGAACTTC TGGAAGTCTT AACAGGCTTT 
GAAACAAATA ACAAATATGA AATCAAGAAC AGCCTTGGGC AGAGGGTTTA CTTTGCGGTG 
GAAGATACGG ACTGCTGCAC TCGAAACTGC TGTGGGGCGT CTAGACCGTT CACCTTGAGG 
ATCCTGGATA ACATGGGCCG AGAAGTCATG ACTCTGGAGA GACCCCTGAG ATGCAGTAGC 
TGCTGCTTCC CCTGCTGCCT CCAGGAGATA GAAATCCAGG CTCCTCCTGG GGTGCCAGTA 
GGTTATGTGA TTCAGACCTG GCACCCATGT CTGCCAAAGT TCACTCTCCA AAATGAGAAG 
AGGCAGGACG TTCTAAAGGT CGTTGGTCCG TGTGTTGTGT GTAGCTGCTG TTCAGACATT 
GACTTTGAGC TCAAATCTCT AGATGAGGAA TCCGTTGTTG GCAAAATTTC CAAGCAGTGG 
TCTGGTTTTG TGAGAGAGGC CTTCACGGAT GCAGATAACT TCGGGATCCA GTTCCCGCTG 
GACCTGGATG TGAAGATGAA AGCTGTGATG CTTGGCGCTT GTTTCCTCAT AGATTTCATG 
TTTTTTGAAA GAACTGGAAA TGAGGAACAA AGGTCAGGAG TATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plscr1 ( XM_006243536.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243598.1 Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31457
Clone ID Related Accession (Same CDS sequence)	XM_006243536.2 , XM_006243537.2
Accession Version	XM_006243536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid scramblase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC ACGGCAAGCA AACCGAGCCT CCCTACCCGG GACCATATTT GCCAGCTATC 
CAAGGACCAC CAGAACATGC TGCTTACCCC ATTCCTCAGG CTGACTACCA AGGGTCCCAG 
GGTCCCTATC CAGGACCGCA GGGTCCATAT CCAGGGCCCC AGGGTCCCTA TGCAGGGCCT 
CAAGGTCCCT ATCCAGGGCC CCAGGGTCCC TATGCAGGGC CCCAAGGTCC CTATCCAGGG 
CCCCAACCTG GCTACCCAGT TCCACCAGGC AGTTATGCAG GTGGTGACCC AAGTGGCTTT 
CCTGTCCAAC ATCAGCCGGC GTATAATCAC CCAGGTGGAC CTGGGGGCAC CCCATGGATG 
CAAGCACCAC CTCCACCCCT GGACTGTCCG CCTGGGCTGG AATACTTAAC GCAGATAGAT 
CAGATTCTGG TTCATCAGCA GATTGAACTT CTGGAAGTCT TAACAGGCTT TGAAACAAAT 
AACAAATATG AAATCAAGAA CAGCCTTGGG CAGAGGGTTT ACTTTGCGGT GGAAGATACG 
GACTGCTGCA CTCGAAACTG CTGTGGGGCG TCTAGACCGT TCACCTTGAG GATCCTGGAT 
AACATGGGCC GAGAAGTCAT GACTCTGGAG AGACCCCTGA GATGCAGTAG CTGCTGCTTC 
CCCTGCTGCC TCCAGGAGAT AGAAATCCAG GCTCCTCCTG GGGTGCCAGT AGGTTATGTG 
ATTCAGACCT GGCACCCATG TCTGCCAAAG TTCACTCTCC AAAATGAGAA GAGGCAGGAC 
GTTCTAAAGG TCGTTGGTCC GTGTGTTGTG TGTAGCTGCT GTTCAGACAT TGACTTTGAG 
CTCAAATCTC TAGATGAGGA ATCCGTTGTT GGCAAAATTT CCAAGCAGTG GTCTGGTTTT 
GTGAGAGAGG CCTTCACGGA TGCAGATAAC TTCGGGATCC AGTTCCCGCT GGACCTGGAT 
GTGAAGATGA AAGCTGTGAT GCTTGGCGCT TGTTTCCTCA TAGATTTCAT GTTTTTTGAA 
AGAACTGGAA ATGAGGAACA AAGGTCAGGA GTATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243598.1
CDS	185..1243
Translation	MEKHGKQTEPPYPGPYLPAIQGPPEHAAYPIPQADYQGSQGPYPGPQGPYPGPQGPYAGPQGPYPGPQGPYAGPQGPYPGPQPGYPVPPGSYAGGDPSGFPVQHQPAYNHPGGPGGTPWMQAPPPPLDCPPGLEYLTQIDQILVHQQIELLEVLTGFETNNKYEIKNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNMGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGVPVGYVIQTWHPCLPKFTLQNEKRQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW

Target ORF information:

RefSeq Version	XM_006243536.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243536.2

ORF Insert Sequence:

ATGGAGAAGC ACGGCAAGCA AACCGAGCCT CCCTACCCGG GACCATATTT GCCAGCTATC 
CAAGGACCAC CAGAACATGC TGCTTACCCC ATTCCTCAGG CTGACTACCA AGGGTCCCAG 
GGTCCCTATC CAGGACCGCA GGGTCCATAT CCAGGGCCCC AGGGTCCCTA TGCAGGGCCT 
CAAGGTCCCT ATCCAGGGCC CCAGGGTCCC TATGCAGGGC CCCAAGGTCC CTATCCAGGG 
CCCCAACCTG GCTACCCAGT TCCACCAGGC AGTTATGCAG GTGGTGACCC AAGTGGCTTT 
CCTGTCCAAC ATCAGCCGGC GTATAATCAC CCAGGTGGAC CTGGGGGCAC CCCATGGATG 
CAAGCACCAC CTCCACCCCT GGACTGTCCG CCTGGGCTGG AATACTTAAC GCAGATAGAT 
CAGATTCTGG TTCATCAGCA GATTGAACTT CTGGAAGTCT TAACAGGCTT TGAAACAAAT 
AACAAATATG AAATCAAGAA CAGCCTTGGG CAGAGGGTTT ACTTTGCGGT GGAAGATACG 
GACTGCTGCA CTCGAAACTG CTGTGGGGCG TCTAGACCGT TCACCTTGAG GATCCTGGAT 
AACATGGGCC GAGAAGTCAT GACTCTGGAG AGACCCCTGA GATGCAGTAG CTGCTGCTTC 
CCCTGCTGCC TCCAGGAGAT AGAAATCCAG GCTCCTCCTG GGGTGCCAGT AGGTTATGTG 
ATTCAGACCT GGCACCCATG TCTGCCAAAG TTCACTCTCC AAAATGAGAA GAGGCAGGAC 
GTTCTAAAGG TCGTTGGTCC GTGTGTTGTG TGTAGCTGCT GTTCAGACAT TGACTTTGAG 
CTCAAATCTC TAGATGAGGA ATCCGTTGTT GGCAAAATTT CCAAGCAGTG GTCTGGTTTT 
GTGAGAGAGG CCTTCACGGA TGCAGATAAC TTCGGGATCC AGTTCCCGCT GGACCTGGAT 
GTGAAGATGA AAGCTGTGAT GCTTGGCGCT TGTTTCCTCA TAGATTTCAT GTTTTTTGAA 
AGAACTGGAA ATGAGGAACA AAGGTCAGGA GTATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plscr1 ( XM_006243537.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243599.1 Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31457
Clone ID Related Accession (Same CDS sequence)	XM_006243536.2 , XM_006243537.2
Accession Version	XM_006243537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholipid scramblase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC ACGGCAAGCA AACCGAGCCT CCCTACCCGG GACCATATTT GCCAGCTATC 
CAAGGACCAC CAGAACATGC TGCTTACCCC ATTCCTCAGG CTGACTACCA AGGGTCCCAG 
GGTCCCTATC CAGGACCGCA GGGTCCATAT CCAGGGCCCC AGGGTCCCTA TGCAGGGCCT 
CAAGGTCCCT ATCCAGGGCC CCAGGGTCCC TATGCAGGGC CCCAAGGTCC CTATCCAGGG 
CCCCAACCTG GCTACCCAGT TCCACCAGGC AGTTATGCAG GTGGTGACCC AAGTGGCTTT 
CCTGTCCAAC ATCAGCCGGC GTATAATCAC CCAGGTGGAC CTGGGGGCAC CCCATGGATG 
CAAGCACCAC CTCCACCCCT GGACTGTCCG CCTGGGCTGG AATACTTAAC GCAGATAGAT 
CAGATTCTGG TTCATCAGCA GATTGAACTT CTGGAAGTCT TAACAGGCTT TGAAACAAAT 
AACAAATATG AAATCAAGAA CAGCCTTGGG CAGAGGGTTT ACTTTGCGGT GGAAGATACG 
GACTGCTGCA CTCGAAACTG CTGTGGGGCG TCTAGACCGT TCACCTTGAG GATCCTGGAT 
AACATGGGCC GAGAAGTCAT GACTCTGGAG AGACCCCTGA GATGCAGTAG CTGCTGCTTC 
CCCTGCTGCC TCCAGGAGAT AGAAATCCAG GCTCCTCCTG GGGTGCCAGT AGGTTATGTG 
ATTCAGACCT GGCACCCATG TCTGCCAAAG TTCACTCTCC AAAATGAGAA GAGGCAGGAC 
GTTCTAAAGG TCGTTGGTCC GTGTGTTGTG TGTAGCTGCT GTTCAGACAT TGACTTTGAG 
CTCAAATCTC TAGATGAGGA ATCCGTTGTT GGCAAAATTT CCAAGCAGTG GTCTGGTTTT 
GTGAGAGAGG CCTTCACGGA TGCAGATAAC TTCGGGATCC AGTTCCCGCT GGACCTGGAT 
GTGAAGATGA AAGCTGTGAT GCTTGGCGCT TGTTTCCTCA TAGATTTCAT GTTTTTTGAA 
AGAACTGGAA ATGAGGAACA AAGGTCAGGA GTATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243599.1
CDS	180..1238
Translation	MEKHGKQTEPPYPGPYLPAIQGPPEHAAYPIPQADYQGSQGPYPGPQGPYPGPQGPYAGPQGPYPGPQGPYAGPQGPYPGPQPGYPVPPGSYAGGDPSGFPVQHQPAYNHPGGPGGTPWMQAPPPPLDCPPGLEYLTQIDQILVHQQIELLEVLTGFETNNKYEIKNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNMGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGVPVGYVIQTWHPCLPKFTLQNEKRQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW

Target ORF information:

RefSeq Version	XM_006243537.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipid scramblase 1 (Plscr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243537.2

ORF Insert Sequence:

ATGGAGAAGC ACGGCAAGCA AACCGAGCCT CCCTACCCGG GACCATATTT GCCAGCTATC 
CAAGGACCAC CAGAACATGC TGCTTACCCC ATTCCTCAGG CTGACTACCA AGGGTCCCAG 
GGTCCCTATC CAGGACCGCA GGGTCCATAT CCAGGGCCCC AGGGTCCCTA TGCAGGGCCT 
CAAGGTCCCT ATCCAGGGCC CCAGGGTCCC TATGCAGGGC CCCAAGGTCC CTATCCAGGG 
CCCCAACCTG GCTACCCAGT TCCACCAGGC AGTTATGCAG GTGGTGACCC AAGTGGCTTT 
CCTGTCCAAC ATCAGCCGGC GTATAATCAC CCAGGTGGAC CTGGGGGCAC CCCATGGATG 
CAAGCACCAC CTCCACCCCT GGACTGTCCG CCTGGGCTGG AATACTTAAC GCAGATAGAT 
CAGATTCTGG TTCATCAGCA GATTGAACTT CTGGAAGTCT TAACAGGCTT TGAAACAAAT 
AACAAATATG AAATCAAGAA CAGCCTTGGG CAGAGGGTTT ACTTTGCGGT GGAAGATACG 
GACTGCTGCA CTCGAAACTG CTGTGGGGCG TCTAGACCGT TCACCTTGAG GATCCTGGAT 
AACATGGGCC GAGAAGTCAT GACTCTGGAG AGACCCCTGA GATGCAGTAG CTGCTGCTTC 
CCCTGCTGCC TCCAGGAGAT AGAAATCCAG GCTCCTCCTG GGGTGCCAGT AGGTTATGTG 
ATTCAGACCT GGCACCCATG TCTGCCAAAG TTCACTCTCC AAAATGAGAA GAGGCAGGAC 
GTTCTAAAGG TCGTTGGTCC GTGTGTTGTG TGTAGCTGCT GTTCAGACAT TGACTTTGAG 
CTCAAATCTC TAGATGAGGA ATCCGTTGTT GGCAAAATTT CCAAGCAGTG GTCTGGTTTT 
GTGAGAGAGG CCTTCACGGA TGCAGATAAC TTCGGGATCC AGTTCCCGCT GGACCTGGAT 
GTGAAGATGA AAGCTGTGAT GCTTGGCGCT TGTTTCCTCA TAGATTTCAT GTTTTTTGAA 
AGAACTGGAA ATGAGGAACA AAGGTCAGGA GTATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
PloS one9(10)e109800(2014)
Kassas A,Moura IC,Yamashita Y,Scheffel J,Gu?rin-Marchand C,Blank U,Sims PJ,Wiedmer T,Monteiro RC,Rivera J,Charles N,Benhamou M

Phospholipid scramblase 1 functionally interacts with angiogenin and regulates angiogenin-enhanced rRNA transcription.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology32(6)1695-706(2013)
Zhu J,Sheng J,Dong H,Kang L,Ang J,Xu Z

Identification of PLSCR1 as a protein that interacts with RELT family members.
Molecular and cellular biochemistry362(1-2)55-63(2012 Mar)
Cusick JK,Mustian A,Jacobs AT,Reyland ME

Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.
The Journal of biological chemistry285(48)37823-37(2010 Nov)
Merregaert J,Van Langen J,Hansen U,Ponsaerts P,El Ghalbzouri A,Steenackers E,Van Ostade X,Sercu S

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Plscr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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