Cry2 cDNA ORF clone, Rattus norvegicus(Norway rat)
| Gene Symbol | Cry2 |
| Entrez Gene ID | 170917 |
| Full Name | cryptochrome circadian regulator 2 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Rattus norvegicus(Norway rat) |
| Genome | |
| Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_133405.2 | NP_596896.2 | cryptochrome-2 |
| Bos taurus (cattle) | LOC509058 | XP_002693610.3 |
| Homo sapiens (human) | CRY2 | NP_066940.2 |
| Pan troglodytes (chimpanzee) | CRY2 | XP_001160595.2 |
| Danio rerio (zebrafish) | cry3 | NP_571861.2 |
| Canis lupus familiaris (dog) | CRY2 | XP_540761.2 |
| Mus musculus (house mouse) | Cry2 | NP_034093.1 |
| Rattus norvegicus (Norway rat) | Cry2 | NP_596896.1 |
| Gallus gallus (chicken) | CRY2 | NP_989575.1 |
| Xenopus tropicalis (tropical clawed frog) | cry2 | XP_002934138.2 |
| CRY2 | XP_001113162.1 |
Related articles in PubMed
Circadian repressors CRY1 and CRY2 broadly interact with nuclear receptors and modulate transcriptional activity.
Kriebs A, Jordan SD, Soto E, Henriksson E, Sandate CR, Vaughan ME, Chan AB, Duglan D, Papp SJ, Huber AL, Afetian ME, Yu RT, Zhao X, Downes M, Evans RM, Lamia KA
Proceedings of the National Academy of Sciences of the United States of America114(33)8776-8781(2017 Aug)
CRY1/2 Selectively Repress PPAR? and Limit Exercise Capacity.
Jordan SD, Kriebs A, Vaughan M, Duglan D, Fan W, Henriksson E, Huber AL, Papp SJ, Nguyen M, Afetian M, Downes M, Yu RT, Kralli A, Evans RM, Lamia KA
Cell metabolism26(1)243-255.e6(2017 Jul)
Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats.
Mattam U, Jagota A
Biogerontology15(3)257-68(2014 Jun)
A novel protein, CHRONO, functions as a core component of the mammalian circadian clock.
Goriki A, Hatanaka F, Myung J, Kim JK, Yoritaka T, Tanoue S, Abe T, Kiyonari H, Fujimoto K, Kato Y, Todo T, Matsubara A, Forger D, Takumi T
PLoS biology12(4)e1001839(2014 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Data show that there is a 24-h rhythm in the expression of Per2 and Cry2 in males and females.
Title: Gender associated circadian oscillations of the clock genes in rat choroid plexus.
We investigated the effect of aging on daily rhythms of various clock genes such as rPer1, rPer2, rCry1, rCry2 and rBmal1 in the SCN of male Wistar rats.
Title: Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats.
N-nitroso-N-methylurea disrupted the expression of Cry2 in mammary glands of Fischer 344 rats
Title: Methylselenocysteine resets the rhythmic expression of circadian and growth-regulatory genes disrupted by nitrosomethylurea in vivo.
cry2 may function in circadian photoreception in the rat brain
Title: Cloning and expression of cryptochrome2 cDNA in the rat.
The following Cry2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cry2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa13704 | |
| Clone ID Related Accession (Same CDS sequence) | NM_133405.2 | |
| Accession Version | NM_133405.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cryptochrome-2 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166416.1. On or before Feb 5, 2014 this sequence version replaced XM_006234547.1, NM_133405.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166416.1, FQ211347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGGCGG CTGCTGTGGT GGCAGCGACG GTCCCCGCGC AGTCGATGGG CGCGGACGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_596896.2 |
| CDS | 3..1787 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_133405.2 |
ORF Insert Sequence:
1 | ATGGCGGCGG CTGCTGTGGT GGCAGCGACG GTCCCCGCGC AGTCGATGGG CGCGGACGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Circadian repressors CRY1 and CRY2 broadly interact with nuclear receptors and modulate transcriptional activity. |
|
CRY1/2 Selectively Repress PPAR? and Limit Exercise Capacity. |
|
Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats. |
|
A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. |