Eif2b5 cDNA ORF clone, Rattus norvegicus(Norway rat)
| Gene Symbol | Eif2b5 |
| Entrez Gene ID | 192234 |
| Full Name | eukaryotic translation initiation factor 2B subunit epsilon |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Rattus norvegicus(Norway rat) |
| Genome | |
| Summary | subunit of eukaryotic initiation factor-2B, which plays a role in eukayrotic initiation factor 2 (eIF-2) recycling in translation initiation [RGD, Feb 2006]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_138866.2 | NP_620221.2 | translation initiation factor eIF-2B subunit epsilon |
| Pan troglodytes (chimpanzee) | EIF2B5 | XP_001139517.1 |
| Gallus gallus (chicken) | EIF2B5 | XP_422755.3 |
| Drosophila melanogaster (fruit fly) | eIF2B-epsilon | NP_996329.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP000082 | XP_001687758.1 |
| Arabidopsis thaliana (thale cress) | AT2G34970 | NP_181042.1 |
| EIF2B5 | XP_001104122.1 | |
| Danio rerio (zebrafish) | eif2b5 | NP_001107069.1 |
| Schizosaccharomyces pombe (fission yeast) | tif225 | NP_594285.1 |
| Arabidopsis thaliana (thale cress) | AT4G18300 | NP_193564.1 |
| Xenopus tropicalis (tropical clawed frog) | eif2b5 | XP_002935185.2 |
| Mus musculus (house mouse) | Eif2b5 | NP_758469.1 |
| Saccharomyces cerevisiae (baker's yeast) | GCD6 | NP_010497.3 |
| Eremothecium gossypii | AGOS_AGL094W | NP_986572.1 |
| Canis lupus familiaris (dog) | EIF2B5 | XP_003640192.1 |
| Bos taurus (cattle) | EIF2B5 | XP_002684924.1 |
| Rattus norvegicus (Norway rat) | Eif2b5 | NP_620221.2 |
| Kluyveromyces lactis | KLLA0A04235g | XP_451187.1 |
| Neurospora crassa | NCU02414 | XP_959596.1 |
| Homo sapiens (human) | EIF2B5 | NP_003898.2 |
| Magnaporthe oryzae (rice blast fungus) | MGG_03517 | XP_003716402.1 |
| Arabidopsis thaliana (thale cress) | AT3G02270 | NP_186876.1 |
| Oryza sativa (rice) | Os02g0812400 | NP_001048478.1 |
Related articles in PubMed
The mechanism of eukaryotic translation initiation: new insights and challenges.
Hinnebusch AG, Lorsch JR
Cold Spring Harbor perspectives in biology4(10)(2012 Oct)
Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.
Tuckow AP, Vary TC, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism299(2)E241-8(2010 Aug)
A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells.
Kantor L, Pinchasi D, Mintz M, Hathout Y, Vanderver A, Elroy-Stein O
PloS one3(11)e3783(2008)
Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis.
Kubica N, Crispino JL, Gallagher JW, Kimball SR, Jefferson LS
The international journal of biochemistry & cell biology40(11)2522-33(2008)
Reduced eIF2alpha phosphorylation and increased proapoptotic proteins in aging.
Hussain SG, Ramaiah KV
Biochemical and biophysical research communications355(2)365-70(2007 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
modulation of eIF2Bepsilon expression may be sufficient to correct deficits in skeletal muscle protein synthesis associated with sepsis and other muscle-wasting conditions
Title: Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.
oligodendroglial-derived cells expressing a mutated eIF2B5 constantly use their stress response mechanism as an adaptation mean in order to survive
Title: A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells.
Data show that activation of mTORC1 is necessary and sufficient to stimulate eIF2Bepsilon mRNA translation.
Title: Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis.
mTOR signaling and eIF2Bepsilon mRNA translation are linked and could have roles in the stimulation of protein synthesis following acute resistance exercise
Title: Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.
The following Eif2b5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2b5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa01807 | |
| Clone ID Related Accession (Same CDS sequence) | NM_138866.2 | |
| Accession Version | NM_138866.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-23 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | translation initiation factor eIF-2B subunit epsilon | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085698.1. On Nov 15, 2004 this sequence version replaced NM_138866.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085698.1, U19516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCAGCCA CTGCGGCGGT TCCCAGCGCT GTAGGCGGCC GAGCGAACAA ACGCGGCGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_620221.2 |
| CDS | 37..2187 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_138866.2 |
ORF Insert Sequence:
1 | ATGGCAGCCA CTGCGGCGGT TCCCAGCGCT GTAGGCGGCC GAGCGAACAA ACGCGGCGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
The mechanism of eukaryotic translation initiation: new insights and challenges. |
|
Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis. |
|
A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells. |
|
Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis. |
|
Reduced eIF2alpha phosphorylation and increased proapoptotic proteins in aging. |