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Prpf19 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Prpf19
Entrez Gene ID 246216
Full Name pre-mRNA processing factor 19
Synonyms Prp19
General protein information
Preferred Names

pre-mRNA processing factor 19

Names

pre-mRNA-processing factor 19
PRP19/PSO4 homolog
PRP19/PSO4 pre-mRNA processing factor 19 homolog
RING-type E3 ubiquitin transferase PRP19
neuronal differentiation-related gene protein
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

1

1q43
Summary human homolog is involved in DNA double strand break repair [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006231014.3 XP_006231076.1 pre-mRNA-processing factor 19 isoform X2
XM_006231015.3 XP_006231077.1 pre-mRNA-processing factor 19 isoform X3
XM_006231013.3 XP_006231075.1 pre-mRNA-processing factor 19 isoform X1
NM_139333.3 NP_647549.2 pre-mRNA-processing factor 19

Pathways from BioSystems


Homologies


Gallus gallus (chicken) PRPF19 NP_001034420.1
PRPF19 XP_001084826.2
Bos taurus (cattle) PRPF19 NP_001069108.1
Xenopus tropicalis (tropical clawed frog) prpf19 NP_001005435.1
Homo sapiens (human) PRPF19 NP_055317.1
Drosophila melanogaster (fruit fly) Prp19 NP_523783.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007217 XP_308568.4
Rattus norvegicus (Norway rat) Prpf19 NP_647549.2
Danio rerio (zebrafish) prp19 NP_958875.1
Arabidopsis thaliana (thale cress) MAC3B NP_850207.4
Pan troglodytes (chimpanzee) PRPF19 XP_001142421.1
Canis lupus familiaris (dog) PRPF19 XP_853782.1
Mus musculus (house mouse) Prpf19 NP_598890.1
Arabidopsis thaliana (thale cress) MAC3A NP_563708.1
Oryza sativa (rice) Os10g0466300 NP_001064804.1

Gene Ontology


Bibliography

Related articles in PubMed

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
Mar?chal A, Li JM, Ji XY, Wu CS, Yazinski SA, Nguyen HD, Liu S, Jim?nez AE, Jin J, Zou L
Molecular cell53(2)235-246(2014 Jan)

Splicing and beyond: the many faces of the Prp19 complex.
Chanarat S, Str??er K
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)

The U-box-type ubiquitin ligase PRP19? regulates astrocyte differentiation via ubiquitination of PTP1B.
Yamada T, Urano-Tashiro Y, Hashi Y, Sakumoto M, Akiyama H, Tashiro F
Brain research152412-25(2013 Aug)

The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
Song EJ, Werner SL, Neubauer J, Stegmeier F, Aspden J, Rio D, Harper JW, Elledge SJ, Kirschner MW, Rape M
Genes & development24(13)1434-47(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results indicate that PRP19beta activates the gp130/Janus kinase (JAK)/STAT signaling pathway during astrocyte differentiation via PTP1B ubiquitination
Title: The U-box-type ubiquitin ligase PRP19? regulates astrocyte differentiation via ubiquitination of PTP1B.

The following Prpf19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12492 NM_139333.3
Latest version!		 Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $293.30
$419.00
ORa23884 XM_006231013.3
Latest version!		 Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa23885 XM_006231014.3
Latest version!		 Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa23886 XM_006231015.3
Latest version!		 Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12492
Clone ID Related Accession (Same CDS sequence) NM_139333.3
Accession Version NM_139333.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-25
Organism Rattus norvegicus(Norway rat)
Product pre-mRNA-processing factor 19
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107669.1. On Oct 31, 2005 this sequence version replaced NM_139333.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107669.1, AB020022.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGATG CAGTCATGCT GCACAGCTTC ACTCTTCGCC AGCAGCTGCA GACAACCCGC 
CAGGAACTGT CCCACGCTCT GTACCAGCAC GATGCTGCCT GCCGAGTCAT TGCCCGGCTC 
ACCAAGGAGG TCACTGCTGC TCGAGAAGCT CTGGCTACTC TGAAACCACA GGCTGGTCTT 
ATTGTACCTC AGGCTGTGCC AAGCTCACAG CCCAGTGTTG TGGGTGCAGG AGAGCCCATG 
GATTTGGGTG AGCTTGTGGG AATGACCCCT GAGATTATCC AGAAGCTTCA AGACAAGGCC 
ACTGTACTAA CCACGGAGCG TAAGAAGAGA GGAAAAACTG TCCCTGAGGA GCTGGTGAAG 
CCTGAAGAGC TCAGCAAGTA CCGGCAGGTG GCATCCCATG TGGGGCTACA CAGTGCTAGC 
ATTCCTGGGA TTCTTGCTCT GGACCTGTGT CCCTCAGACA CCAACAAGAT TCTTACTGGT 
GGGGCAGATA AAAACGTTGT TGTCTTTGAT AAGAGTACTG AGCAAATCCT GGCCACTCTC 
AAAGGCCATA CCAAGAAGGT CACCAGTGTG GTGTTTCATC CTTCTCAGGA ATTGGTGTTT 
TCTGCGTCCC CTGATGCTAC TATCAGGATT TGGTCCGTCC CGAACACTTC TTGTGTACAG 
GTTGTTCGGG CCCATGAGAG TGCGGTGACA GGACTCAGCC TCCATGCCAC TGGAGACTAT 
CTACTGAGCT CCTCTGATGA TCAGTACTGG GCGTTCTCTG ACATTCAGAC AGGGCGTGTG 
CTCACCAAGG TGACAGATGA GACTTCCGGC TGCTCTCTTA CCTGTGCACA GTTCCACCCT 
GATGGCCTCA TCTTTGGAAC AGGAACCATG GACTCCCAGA TCAAGATCTG GGACTTGAAG 
GAACGCACCA ATGTGGCGAA CTTCCCTGGC CACTCCGGCC CCATTACCAG CATTGCCTTC 
TCTGAGAATG GGTACTACCT GGCCACGGCA GCTGATGACT CCTCAGTCAA GCTCTGGGAC 
TTACGCAAGT TGAAGAACTT CAAGACATTG CAGCTGGACA ACAACTTTGA GGTGAAGTCA 
CTAATCTTTG ACCAGAGTGG CACCTACCTG GCGCTTGGGG GTACAGATGT CCAGATCTAC 
ATCTGCAAAC AGTGGACAGA GATTCTTCAC TTTACAGAGC ATAGTGGCCT GACCACTGGA 
GTGGCCTTTG GGCACCACGC CAAGTTCATC GCTTCAACCG GCATGGACAG GAGCCTCAAA 
TTCTACAGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_647549.2
CDS188..1702
Translation

Target ORF information:

RefSeq Version NM_139333.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_139333.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGATG CAGTCATGCT GCACAGCTTC ACTCTTCGCC AGCAGCTGCA GACAACCCGC 
CAGGAACTGT CCCACGCTCT GTACCAGCAC GATGCTGCCT GCCGAGTCAT TGCCCGGCTC 
ACCAAGGAGG TCACTGCTGC TCGAGAAGCT CTGGCTACTC TGAAACCACA GGCTGGTCTT 
ATTGTACCTC AGGCTGTGCC AAGCTCACAG CCCAGTGTTG TGGGTGCAGG AGAGCCCATG 
GATTTGGGTG AGCTTGTGGG AATGACCCCT GAGATTATCC AGAAGCTTCA AGACAAGGCC 
ACTGTACTAA CCACGGAGCG TAAGAAGAGA GGAAAAACTG TCCCTGAGGA GCTGGTGAAG 
CCTGAAGAGC TCAGCAAGTA CCGGCAGGTG GCATCCCATG TGGGGCTACA CAGTGCTAGC 
ATTCCTGGGA TTCTTGCTCT GGACCTGTGT CCCTCAGACA CCAACAAGAT TCTTACTGGT 
GGGGCAGATA AAAACGTTGT TGTCTTTGAT AAGAGTACTG AGCAAATCCT GGCCACTCTC 
AAAGGCCATA CCAAGAAGGT CACCAGTGTG GTGTTTCATC CTTCTCAGGA ATTGGTGTTT 
TCTGCGTCCC CTGATGCTAC TATCAGGATT TGGTCCGTCC CGAACACTTC TTGTGTACAG 
GTTGTTCGGG CCCATGAGAG TGCGGTGACA GGACTCAGCC TCCATGCCAC TGGAGACTAT 
CTACTGAGCT CCTCTGATGA TCAGTACTGG GCGTTCTCTG ACATTCAGAC AGGGCGTGTG 
CTCACCAAGG TGACAGATGA GACTTCCGGC TGCTCTCTTA CCTGTGCACA GTTCCACCCT 
GATGGCCTCA TCTTTGGAAC AGGAACCATG GACTCCCAGA TCAAGATCTG GGACTTGAAG 
GAACGCACCA ATGTGGCGAA CTTCCCTGGC CACTCCGGCC CCATTACCAG CATTGCCTTC 
TCTGAGAATG GGTACTACCT GGCCACGGCA GCTGATGACT CCTCAGTCAA GCTCTGGGAC 
TTACGCAAGT TGAAGAACTT CAAGACATTG CAGCTGGACA ACAACTTTGA GGTGAAGTCA 
CTAATCTTTG ACCAGAGTGG CACCTACCTG GCGCTTGGGG GTACAGATGT CCAGATCTAC 
ATCTGCAAAC AGTGGACAGA GATTCTTCAC TTTACAGAGC ATAGTGGCCT GACCACTGGA 
GTGGCCTTTG GGCACCACGC CAAGTTCATC GCTTCAACCG GCATGGACAG GAGCCTCAAA 
TTCTACAGTC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa23884
Clone ID Related Accession (Same CDS sequence) XM_006231013.3
Accession Version XM_006231013.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1602bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product pre-mRNA-processing factor 19 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGGTT GGCCGAATTC TCCGGCTCTC CCCCAGTCCT CACAGTGGCC TACCCTCTCC 
CAGGATGCAG TCATGCTGCA CAGCTTCACT CTTCGCCAGC AGCTGCAGAC AACCCGCCAG 
GAACTGTCCC ACGCTCTGTA CCAGCACGAT GCTGCCTGCC GAGTCATTGC CCGGCTCACC 
AAGGAGGTCA CTGCTGCTCG AGAAGCTCTG GCTACTCTGA AACCACAGGC TGGTCTTATT 
GTACCTCAGG CTGTGCCAAG CTCACAGCCC AGTGTTGTGG GTGCAGGAGA GCCCATGGAT 
TTGGGTGAGC TTGTGGGAAT GACCCCTGAG ATTATCCAGA AGCTTCAAGA CAAGGCCACT 
GTACTAACCA CGGAGCGTAA GAAGAGAGGA AAAACTGTCC CTGAGGAGCT GGTGAAGCCT 
GAAGAGCTCA GCAAGTACCG GCAGGTGGCA TCCCATGTGG GGCTACACAG TGCTAGCATT 
CCTGGGATTC TTGCTCTGGA CCTGTGTCCC TCAGACACCA ACAAGATTCT TACTGGTGGG 
GCAGATAAAA ACGTTGTTGT CTTTGATAAG AGTACTGAGC AAATCCTGGC CACTCTCAAA 
GGCCATACCA AGAAGGTCAC CAGTGTGGTG TTTCATCCTT CTCAGGAATT GGTGTTTTCT 
GCGTCCCCTG ATGCTACTAT CAGGATTTGG TCCGTCCCGA ACACTTCTTG TGTACAGGTT 
GTTCGGGCCC ATGAGAGTGC GGTGACAGGA CTCAGCCTCC ATGCCACTGG AGACTATCTA 
CTGAGCTCCT CTGATGATCA GTACTGGGCG TTCTCTGACA TTCAGACAGG GCGTGTGCTC 
ACCAAGGTGA CAGATGAGAC TTCCGGCTGC TCTCTTACCT GTGCACAGTT CCACCCTGAT 
GGCCTCATCT TTGGAACAGG AACCATGGAC TCCCAGATCA AGATCTGGGA CTTGAAGGAA 
CGCACCAATG TGGCGAACTT CCCTGGCCAC TCCGGCCCCA TTACCAGCAT TGCCTTCTCT 
GAGAATGGGT ACTACCTGGC CACGGCAGCT GATGACTCCT CAGTCAAGCT CTGGGACTTA 
CGCAAGTTGA AGAACTTCAA GACATTGCAG CTGGACAACA ACTTTGAGGT GAAGTCACTA 
ATCTTTGACC AGAGTGGCAC CTACCTGGCG CTTGGGGGTA CAGATGTCCA GATCTACATC 
TGCAAACAGT GGACAGAGAT TCTTCACTTT ACAGAGCATT GTGGCCCAGG AAAAGTACTT 
GTGAGTCTGT CCCAGAGTAC TGCTACTAGC TGGCCTCTCC CAAGCTGTGT GTTGAGGAGC 
AAACTGATCT CCCTGGTGTT CCAGGAAAAT TGGAATACAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006231075.1
CDS228..1829
Translation

Target ORF information:

RefSeq Version XM_006231013.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006231013.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGGTT GGCCGAATTC TCCGGCTCTC CCCCAGTCCT CACAGTGGCC TACCCTCTCC 
CAGGATGCAG TCATGCTGCA CAGCTTCACT CTTCGCCAGC AGCTGCAGAC AACCCGCCAG 
GAACTGTCCC ACGCTCTGTA CCAGCACGAT GCTGCCTGCC GAGTCATTGC CCGGCTCACC 
AAGGAGGTCA CTGCTGCTCG AGAAGCTCTG GCTACTCTGA AACCACAGGC TGGTCTTATT 
GTACCTCAGG CTGTGCCAAG CTCACAGCCC AGTGTTGTGG GTGCAGGAGA GCCCATGGAT 
TTGGGTGAGC TTGTGGGAAT GACCCCTGAG ATTATCCAGA AGCTTCAAGA CAAGGCCACT 
GTACTAACCA CGGAGCGTAA GAAGAGAGGA AAAACTGTCC CTGAGGAGCT GGTGAAGCCT 
GAAGAGCTCA GCAAGTACCG GCAGGTGGCA TCCCATGTGG GGCTACACAG TGCTAGCATT 
CCTGGGATTC TTGCTCTGGA CCTGTGTCCC TCAGACACCA ACAAGATTCT TACTGGTGGG 
GCAGATAAAA ACGTTGTTGT CTTTGATAAG AGTACTGAGC AAATCCTGGC CACTCTCAAA 
GGCCATACCA AGAAGGTCAC CAGTGTGGTG TTTCATCCTT CTCAGGAATT GGTGTTTTCT 
GCGTCCCCTG ATGCTACTAT CAGGATTTGG TCCGTCCCGA ACACTTCTTG TGTACAGGTT 
GTTCGGGCCC ATGAGAGTGC GGTGACAGGA CTCAGCCTCC ATGCCACTGG AGACTATCTA 
CTGAGCTCCT CTGATGATCA GTACTGGGCG TTCTCTGACA TTCAGACAGG GCGTGTGCTC 
ACCAAGGTGA CAGATGAGAC TTCCGGCTGC TCTCTTACCT GTGCACAGTT CCACCCTGAT 
GGCCTCATCT TTGGAACAGG AACCATGGAC TCCCAGATCA AGATCTGGGA CTTGAAGGAA 
CGCACCAATG TGGCGAACTT CCCTGGCCAC TCCGGCCCCA TTACCAGCAT TGCCTTCTCT 
GAGAATGGGT ACTACCTGGC CACGGCAGCT GATGACTCCT CAGTCAAGCT CTGGGACTTA 
CGCAAGTTGA AGAACTTCAA GACATTGCAG CTGGACAACA ACTTTGAGGT GAAGTCACTA 
ATCTTTGACC AGAGTGGCAC CTACCTGGCG CTTGGGGGTA CAGATGTCCA GATCTACATC 
TGCAAACAGT GGACAGAGAT TCTTCACTTT ACAGAGCATT GTGGCCCAGG AAAAGTACTT 
GTGAGTCTGT CCCAGAGTAC TGCTACTAGC TGGCCTCTCC CAAGCTGTGT GTTGAGGAGC 
AAACTGATCT CCCTGGTGTT CCAGGAAAAT TGGAATACAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa23885
Clone ID Related Accession (Same CDS sequence) XM_006231014.3
Accession Version XM_006231014.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product pre-mRNA-processing factor 19 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGGTT GGCCGAATTC TCCGGCTCTC CCCCAGTCCT CACAGTGGCC TACCCTCTCC 
CAGGATGCAG TCATGCTGCA CAGCTTCACT CTTCGCCAGC AGCTGCAGAC AACCCGCCAG 
GAACTGTCCC ACGCTCTGTA CCAGCACGAT GCTGCCTGCC GAGTCATTGC CCGGCTCACC 
AAGGAGGTCA CTGCTGCTCG AGAAGCTCTG GCTACTCTGA AACCACAGGC TGGTCTTATT 
GTACCTCAGG CTGTGCCAAG CTCACAGCCC AGTGTTGTGG GTGCAGGAGA GCCCATGGAT 
TTGGGTGAGC TTGTGGGAAT GACCCCTGAG ATTATCCAGA AGCTTCAAGA CAAGGCCACT 
GTACTAACCA CGGAGCGTAA GAAGAGAGGA AAAACTGTCC CTGAGGAGCT GGTGAAGCCT 
GAAGAGCTCA GCAAGTACCG GCAGGTGGCA TCCCATGTGG GGCTACACAG TGCTAGCATT 
CCTGGGATTC TTGCTCTGGA CCTGTGTCCC TCAGACACCA ACAAGATTCT TACTGGTGGG 
GCAGATAAAA ACGTTGTTGT CTTTGATAAG AGTACTGAGC AAATCCTGGC CACTCTCAAA 
GGCCATACCA AGAAGGTCAC CAGTGTGGTG TTTCATCCTT CTCAGGAATT GGTGTTTTCT 
GCGTCCCCTG ATGCTACTAT CAGGATTTGG TCCGTCCCGA ACACTTCTTG TGTACAGGTT 
GTTCGGGCCC ATGAGAGTGC GGTGACAGGA CTCAGCCTCC ATGCCACTGG AGACTATCTA 
CTGAGCTCCT CTGATGATCA GTACTGGGCG TTCTCTGACA TTCAGACAGG GCGTGTGCTC 
ACCAAGGTGA CAGATGAGAC TTCCGGCTGC TCTCTTACCT GTGCACAGTT CCACCCTGAT 
GGCCTCATCT TTGGAACAGG AACCATGGAC TCCCAGATCA AGATCTGGGA CTTGAAGGAA 
CGCACCAATG TGGCGAACTT CCCTGGCCAC TCCGGCCCCA TTACCAGCAT TGCCTTCTCT 
GAGAATGGGT ACTACCTGGC CACGGCAGCT GATGACTCCT CAGTCAAGCT CTGGGACTTA 
CGCAAGTTGA AGAACTTCAA GACATTGCAG CTGGACAACA ACTTTGAGGT GAAGTCACTA 
ATCTTTGACC AGAGTGGCAC CTACCTGGCG CTTGGGGGTA CAGATGTCCA GATCTACATC 
TGCAAACAGT GGACAGAGAT TCTTCACTTT ACAGAGCATA GTGGCCTGAC CACTGGAGTG 
GCCTTTGGGC ACCACGCCAA GTTCATCGCT TCAACCGGCA TGGACAGGAG CCTCAAATTC 
TACAGTCTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006231076.1
CDS226..1797
Translation

Target ORF information:

RefSeq Version XM_006231014.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006231014.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGGTT GGCCGAATTC TCCGGCTCTC CCCCAGTCCT CACAGTGGCC TACCCTCTCC 
CAGGATGCAG TCATGCTGCA CAGCTTCACT CTTCGCCAGC AGCTGCAGAC AACCCGCCAG 
GAACTGTCCC ACGCTCTGTA CCAGCACGAT GCTGCCTGCC GAGTCATTGC CCGGCTCACC 
AAGGAGGTCA CTGCTGCTCG AGAAGCTCTG GCTACTCTGA AACCACAGGC TGGTCTTATT 
GTACCTCAGG CTGTGCCAAG CTCACAGCCC AGTGTTGTGG GTGCAGGAGA GCCCATGGAT 
TTGGGTGAGC TTGTGGGAAT GACCCCTGAG ATTATCCAGA AGCTTCAAGA CAAGGCCACT 
GTACTAACCA CGGAGCGTAA GAAGAGAGGA AAAACTGTCC CTGAGGAGCT GGTGAAGCCT 
GAAGAGCTCA GCAAGTACCG GCAGGTGGCA TCCCATGTGG GGCTACACAG TGCTAGCATT 
CCTGGGATTC TTGCTCTGGA CCTGTGTCCC TCAGACACCA ACAAGATTCT TACTGGTGGG 
GCAGATAAAA ACGTTGTTGT CTTTGATAAG AGTACTGAGC AAATCCTGGC CACTCTCAAA 
GGCCATACCA AGAAGGTCAC CAGTGTGGTG TTTCATCCTT CTCAGGAATT GGTGTTTTCT 
GCGTCCCCTG ATGCTACTAT CAGGATTTGG TCCGTCCCGA ACACTTCTTG TGTACAGGTT 
GTTCGGGCCC ATGAGAGTGC GGTGACAGGA CTCAGCCTCC ATGCCACTGG AGACTATCTA 
CTGAGCTCCT CTGATGATCA GTACTGGGCG TTCTCTGACA TTCAGACAGG GCGTGTGCTC 
ACCAAGGTGA CAGATGAGAC TTCCGGCTGC TCTCTTACCT GTGCACAGTT CCACCCTGAT 
GGCCTCATCT TTGGAACAGG AACCATGGAC TCCCAGATCA AGATCTGGGA CTTGAAGGAA 
CGCACCAATG TGGCGAACTT CCCTGGCCAC TCCGGCCCCA TTACCAGCAT TGCCTTCTCT 
GAGAATGGGT ACTACCTGGC CACGGCAGCT GATGACTCCT CAGTCAAGCT CTGGGACTTA 
CGCAAGTTGA AGAACTTCAA GACATTGCAG CTGGACAACA ACTTTGAGGT GAAGTCACTA 
ATCTTTGACC AGAGTGGCAC CTACCTGGCG CTTGGGGGTA CAGATGTCCA GATCTACATC 
TGCAAACAGT GGACAGAGAT TCTTCACTTT ACAGAGCATA GTGGCCTGAC CACTGGAGTG 
GCCTTTGGGC ACCACGCCAA GTTCATCGCT TCAACCGGCA TGGACAGGAG CCTCAAATTC 
TACAGTCTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa23886
Clone ID Related Accession (Same CDS sequence) XM_006231015.3
Accession Version XM_006231015.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product pre-mRNA-processing factor 19 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGATG CAGTCATGCT GCACAGCTTC ACTCTTCGCC AGCAGCTGCA GACAACCCGC 
CAGGAACTGT CCCACGCTCT GTACCAGCAC GATGCTGCCT GCCGAGTCAT TGCCCGGCTC 
ACCAAGGAGG TCACTGCTGC TCGAGAAGCT CTGGCTACTC TGAAACCACA GGCTGGTCTT 
ATTGTACCTC AGGCTGTGCC AAGCTCACAG CCCAGTGTTG TGGGTGCAGG AGAGCCCATG 
GATTTGGGTG AGCTTGTGGG AATGACCCCT GAGATTATCC AGAAGCTTCA AGACAAGGCC 
ACTGTACTAA CCACGGAGCG TAAGAAGAGA GGAAAAACTG TCCCTGAGGA GCTGGTGAAG 
CCTGAAGAGC TCAGCAAGTA CCGGCAGGTG GCATCCCATG TGGGGCTACA CAGTGCTAGC 
ATTCCTGGGA TTCTTGCTCT GGACCTGTGT CCCTCAGACA CCAACAAGAT TCTTACTGGT 
GGGGCAGATA AAAACGTTGT TGTCTTTGAT AAGAGTACTG AGCAAATCCT GGCCACTCTC 
AAAGGCCATA CCAAGAAGGT CACCAGTGTG GTGTTTCATC CTTCTCAGGA ATTGGTGTTT 
TCTGCGTCCC CTGATGCTAC TATCAGGATT TGGTCCGTCC CGAACACTTC TTGTGTACAG 
GTTGTTCGGG CCCATGAGAG TGCGGTGACA GGACTCAGCC TCCATGCCAC TGGAGACTAT 
CTACTGAGCT CCTCTGATGA TCAGTACTGG GCGTTCTCTG ACATTCAGAC AGGGCGTGTG 
CTCACCAAGG TGACAGATGA GACTTCCGGC TGCTCTCTTA CCTGTGCACA GTTCCACCCT 
GATGGCCTCA TCTTTGGAAC AGGAACCATG GACTCCCAGA TCAAGATCTG GGACTTGAAG 
GAACGCACCA ATGTGGCGAA CTTCCCTGGC CACTCCGGCC CCATTACCAG CATTGCCTTC 
TCTGAGAATG GGTACTACCT GGCCACGGCA GCTGATGACT CCTCAGTCAA GCTCTGGGAC 
TTACGCAAGT TGAAGAACTT CAAGACATTG CAGCTGGACA ACAACTTTGA GGTGAAGTCA 
CTAATCTTTG ACCAGAGTGG CACCTACCTG GCGCTTGGGG GTACAGATGT CCAGATCTAC 
ATCTGCAAAC AGTGGACAGA GATTCTTCAC TTTACAGAGC ATTGTGGCCC AGGAAAAGTA 
CTTGTGAGTC TGTCCCAGAG TACTGCTACT AGCTGGCCTC TCCCAAGCTG TGTGTTGAGG 
AGCAAACTGA TCTCCCTGGT GTTCCAGGAA AATTGGAATA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006231077.1
CDS227..1771
Translation

Target ORF information:

RefSeq Version XM_006231015.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus pre-mRNA processing factor 19 (Prpf19), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006231015.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCCTGA TCTGCTCGAT CTCCAACGAA GTGCCAGAGC ACCCATGCGT GTCCCCTGTC 
TCTAATCATG TGTATGAGCG GCGACTCATT GAGAAGTACA TTGCAGAGAA TGGCACAGAT 
CCTATCAACA ACCAGCCTCT CTCAGAGGAG CAGCTCATCG ACATCAAAGT TGCTCATCCA 
ATCCGGCCCA AGCCTCCCTC TGCCACCAGC ATCCCAGCCA TTCTGAAAGC CTTGCAGGAT 
GAGTGGGATG CAGTCATGCT GCACAGCTTC ACTCTTCGCC AGCAGCTGCA GACAACCCGC 
CAGGAACTGT CCCACGCTCT GTACCAGCAC GATGCTGCCT GCCGAGTCAT TGCCCGGCTC 
ACCAAGGAGG TCACTGCTGC TCGAGAAGCT CTGGCTACTC TGAAACCACA GGCTGGTCTT 
ATTGTACCTC AGGCTGTGCC AAGCTCACAG CCCAGTGTTG TGGGTGCAGG AGAGCCCATG 
GATTTGGGTG AGCTTGTGGG AATGACCCCT GAGATTATCC AGAAGCTTCA AGACAAGGCC 
ACTGTACTAA CCACGGAGCG TAAGAAGAGA GGAAAAACTG TCCCTGAGGA GCTGGTGAAG 
CCTGAAGAGC TCAGCAAGTA CCGGCAGGTG GCATCCCATG TGGGGCTACA CAGTGCTAGC 
ATTCCTGGGA TTCTTGCTCT GGACCTGTGT CCCTCAGACA CCAACAAGAT TCTTACTGGT 
GGGGCAGATA AAAACGTTGT TGTCTTTGAT AAGAGTACTG AGCAAATCCT GGCCACTCTC 
AAAGGCCATA CCAAGAAGGT CACCAGTGTG GTGTTTCATC CTTCTCAGGA ATTGGTGTTT 
TCTGCGTCCC CTGATGCTAC TATCAGGATT TGGTCCGTCC CGAACACTTC TTGTGTACAG 
GTTGTTCGGG CCCATGAGAG TGCGGTGACA GGACTCAGCC TCCATGCCAC TGGAGACTAT 
CTACTGAGCT CCTCTGATGA TCAGTACTGG GCGTTCTCTG ACATTCAGAC AGGGCGTGTG 
CTCACCAAGG TGACAGATGA GACTTCCGGC TGCTCTCTTA CCTGTGCACA GTTCCACCCT 
GATGGCCTCA TCTTTGGAAC AGGAACCATG GACTCCCAGA TCAAGATCTG GGACTTGAAG 
GAACGCACCA ATGTGGCGAA CTTCCCTGGC CACTCCGGCC CCATTACCAG CATTGCCTTC 
TCTGAGAATG GGTACTACCT GGCCACGGCA GCTGATGACT CCTCAGTCAA GCTCTGGGAC 
TTACGCAAGT TGAAGAACTT CAAGACATTG CAGCTGGACA ACAACTTTGA GGTGAAGTCA 
CTAATCTTTG ACCAGAGTGG CACCTACCTG GCGCTTGGGG GTACAGATGT CCAGATCTAC 
ATCTGCAAAC AGTGGACAGA GATTCTTCAC TTTACAGAGC ATTGTGGCCC AGGAAAAGTA 
CTTGTGAGTC TGTCCCAGAG TACTGCTACT AGCTGGCCTC TCCCAAGCTG TGTGTTGAGG 
AGCAAACTGA TCTCCCTGGT GTTCCAGGAA AATTGGAATA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R


book

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
Molecular cell53(2)235-246(2014 Jan)
Mar?chal A,Li JM,Ji XY,Wu CS,Yazinski SA,Nguyen HD,Liu S,Jim?nez AE,Jin J,Zou L


book

Splicing and beyond: the many faces of the Prp19 complex.
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)
Chanarat S,Str??er K


book

The U-box-type ubiquitin ligase PRP19? regulates astrocyte differentiation via ubiquitination of PTP1B.
Brain research152412-25(2013 Aug)
Yamada T,Urano-Tashiro Y,Hashi Y,Sakumoto M,Akiyama H,Tashiro F


book

The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
Genes & development24(13)1434-47(2010 Jul)
Song EJ,Werner SL,Neubauer J,Stegmeier F,Aspden J,Rio D,Harper JW,Elledge SJ,Kirschner MW,Rape M