Pmp22 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pmp22
Entrez Gene ID	24660
Full Name	peripheral myelin protein 22
Synonyms	Gas-3
General protein information	Preferred Names peripheral myelin protein 22 Names peripheral myelin protein 22 PMP-22 SAG SR13 myelin protein schwann cell membrane glycoprotein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q23
Summary	mediates Schwann cell growth and peripheral myelin compaction; human homolog gene duplication causes Charcot-Marie-Tooth 1A (CMT1A) neuropathy [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017037.1	NP_058733.1	peripheral myelin protein 22
XM_006246584.3	XP_006246646.1	peripheral myelin protein 22 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PMP22	NP_000295.1
Bos taurus (cattle)	PMP22	NP_001094626.1
Rattus norvegicus (Norway rat)	Pmp22	NP_058733.1
Gallus gallus (chicken)	PMP22	NP_001264001.1
	PMP22	XP_001082754.1
Mus musculus (house mouse)	Pmp22	NP_032911.1
Pan troglodytes (chimpanzee)	PMP22	XP_001162361.1
Canis lupus familiaris (dog)	PMP22	XP_003434676.1
Danio rerio (zebrafish)	pmp22b	NP_001035445.1
Xenopus tropicalis (tropical clawed frog)	pmp22	NP_001025552.1

Gene Ontology
Bibliography

Related articles in PubMed

A dual role for Integrin ?6?4 in modulating Hereditary Neuropathy with liability to Pressure Palsies.
Poitelon Y, Matafora V, Silvestri N, Zambroni D, McGarry C, Serghany N, Rush T, Vizzuso D, Court FA, Bachi A, Wrabetz L, Feltri ML
Journal of neurochemistry(2018 Jan)

Pmp22 mutant allele-specific siRNA alleviates demyelinating neuropathic phenotype in vivo.
Lee JS, Chang EH, Koo OJ, Jwa DH, Mo WM, Kwak G, Moon HW, Park HT, Hong YB, Choi BO
Neurobiology of disease10099-107(2017 Apr)

Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP.
Hu B, Arpag S, Zhang X, M?bius W, Werner H, Sosinsky G, Ellisman M, Zhang Y, Hamilton A, Chernoff J, Li J
PLoS genetics12(9)e1006290(2016 Sep)

Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.
Lim H, Sharoukhov D, Kassim I, Zhang Y, Salzer JL, Melendez-Vasquez CV
Proceedings of the National Academy of Sciences of the United States of America111(50)18025-30(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel MDA-7/IL-24-GAS3-beta1integrin-fibronectin signaling pathway that suppresses breast cancer growth, is reported.
Title: mda-7/IL-24 expression inhibits breast cancer through upregulation of growth arrest-specific gene 3 (gas3) and disruption of ?1 integrin function.

Enhanced PMP22 protein expression and morphological improvements coincide with the abolishment of thermal and mechanical hypersensitivity and pain alleviation after crush injury of a myelinated peripheral nerve.
Title: Dorsal root ganglion application of muscimol prevents hyperalgesia and stimulates myelin protein expression after sciatic nerve injury in rats.

IL-6 can enhance PMP22 production in Schwann cells via a JAK2-dependent pathway by probably activating STAT3 and thus may contribute to myelination.
Title: Interleukin-6 upregulates the expression of PMP22 in cultured rat Schwann cells via a JAK2-dependent pathway.

Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.

miR-29a represses expression of PMP22; analyses of miR-29 & PMP22 in sciatic nerves reveal inverse relationship, developmentally & in post-crush injury; PMP22 identified as target of miRNAs & suggest miRNAs regulate Schwann cell myelin expression
Title: Peripheral myelin protein 22 is regulated post-transcriptionally by miRNA-29a.

The following Pmp22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pmp22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12931	NM_017037.1 Latest version!	Rattus norvegicus peripheral myelin protein 22 (Pmp22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33230	XM_006246584.3 Latest version!	Rattus norvegicus peripheral myelin protein 22 (Pmp22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pmp22 ( NM_017037.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058733.1 Rattus norvegicus peripheral myelin protein 22 (Pmp22), mRNA.

Price & Availability↑

CloneID	ORa12931
Clone ID Related Accession (Same CDS sequence)	NM_017037.1
Accession Version	NM_017037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	peripheral myelin protein 22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X62431.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X62431.1, FM072726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCTAC TCTTGTTGGG GATCCTGTTC CTTCACATCG CGGTGCTAGT GTTGCTCTTC 
GTCTCCACCA TCGTCAGCCA ATGGCTCGAG GGCAATGGAC ACAGGACTGA TCTCTGGCAG 
AACTGTACCA CATCCGCCTT GGGAGCCGTC CAGCACTGCT ACTCCTCATC TGTGAGCGAA 
TGGCTTCAGT CTGTCCAGGC CACCATGATC CTGTCTGTCA TCTTCAGCGT CCTGTCCCTG 
TTCCTGTTCT TCTGCCAGCT CTTCACTCTC ACCAAAGGCG GCCGCTTTTA CATCACTGGA 
GTCTTCCAAA TCCTTGCTGG TCTGTGTGTG ATGAGTGCAG CGGCCATCTA CACAGTGAGA 
CACAGTGAGT GGCATGTCAA CAACGACTAC TCCTATGGCT TTGCTTACAT CCTGGCCTGG 
GTGGCTTTCC CGCTGGCCCT CCTTAGTGGC ATCATCTACG TGATCCTGCG GAAACGCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058733.1
CDS	208..690
Translation	MLLLLLGILFLHIAVLVLLFVSTIVSQWLEGNGHRTDLWQNCTTSALGAVQHCYSSSVSEWLQSVQATMILSVIFSVLSLFLFFCQLFTLTKGGRFYITGVFQILAGLCVMSAAAIYTVRHSEWHVNNDYSYGFAYILAWVAFPLALLSGIIYVILRKRE

Target ORF information:

RefSeq Version	NM_017037.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peripheral myelin protein 22 (Pmp22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017037.1

ORF Insert Sequence:

ATGCTTCTAC TCTTGTTGGG GATCCTGTTC CTTCACATCG CGGTGCTAGT GTTGCTCTTC 
GTCTCCACCA TCGTCAGCCA ATGGCTCGAG GGCAATGGAC ACAGGACTGA TCTCTGGCAG 
AACTGTACCA CATCCGCCTT GGGAGCCGTC CAGCACTGCT ACTCCTCATC TGTGAGCGAA 
TGGCTTCAGT CTGTCCAGGC CACCATGATC CTGTCTGTCA TCTTCAGCGT CCTGTCCCTG 
TTCCTGTTCT TCTGCCAGCT CTTCACTCTC ACCAAAGGCG GCCGCTTTTA CATCACTGGA 
GTCTTCCAAA TCCTTGCTGG TCTGTGTGTG ATGAGTGCAG CGGCCATCTA CACAGTGAGA 
CACAGTGAGT GGCATGTCAA CAACGACTAC TCCTATGGCT TTGCTTACAT CCTGGCCTGG 
GTGGCTTTCC CGCTGGCCCT CCTTAGTGGC ATCATCTACG TGATCCTGCG GAAACGCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pmp22 ( XM_006246584.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246646.1 Rattus norvegicus peripheral myelin protein 22 (Pmp22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33230
Clone ID Related Accession (Same CDS sequence)	XM_006246584.3
Accession Version	XM_006246584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	peripheral myelin protein 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTAC TCTTGTTGGG GATCCTGTTC CTTCACATCG CGGTGCTAGT GTTGCTCTTC 
GTCTCCACCA TCGTCAGCCA ATGGCTCGTG GGCAATGGAC ACAGGACTGA TCTCTGGCAG 
AACTGTACCA CATCCGCCTT GGGAGCCGTC CAGCACTGCT ACTCCTCATC TGTGAGCGAA 
TGGCTTCAGT CTGTCCAGGC CACCATGATC CTGTCTGTCA TCTTCAGCGT CCTGTCCCTG 
TTCCTGTTCT TCTGCCAGCT CTTCACTCTC ACCAAAGGCG GCCGCTTTTA CATCACTGGA 
GTCTTCCAAA TCCTTGCTGG TCTGTGTGTG ATGAGTGCAG CGGCCATCTA CACAGTGAGA 
CACAGTGAGT GGCATGTCAA CAACGACTAC TCCTATGGCT TTGCTTACAT CCTGGCCTGG 
GTGGCTTTCC CGCTGGCCCT CCTTAGTGGC ATCATCTACG TGATCCTGCG GAAACGCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246646.1
CDS	196..678
Translation	MLLLLLGILFLHIAVLVLLFVSTIVSQWLVGNGHRTDLWQNCTTSALGAVQHCYSSSVSEWLQSVQATMILSVIFSVLSLFLFFCQLFTLTKGGRFYITGVFQILAGLCVMSAAAIYTVRHSEWHVNNDYSYGFAYILAWVAFPLALLSGIIYVILRKRE

Target ORF information:

RefSeq Version	XM_006246584.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus peripheral myelin protein 22 (Pmp22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246584.3

ORF Insert Sequence:

ATGCTTCTAC TCTTGTTGGG GATCCTGTTC CTTCACATCG CGGTGCTAGT GTTGCTCTTC 
GTCTCCACCA TCGTCAGCCA ATGGCTCGTG GGCAATGGAC ACAGGACTGA TCTCTGGCAG 
AACTGTACCA CATCCGCCTT GGGAGCCGTC CAGCACTGCT ACTCCTCATC TGTGAGCGAA 
TGGCTTCAGT CTGTCCAGGC CACCATGATC CTGTCTGTCA TCTTCAGCGT CCTGTCCCTG 
TTCCTGTTCT TCTGCCAGCT CTTCACTCTC ACCAAAGGCG GCCGCTTTTA CATCACTGGA 
GTCTTCCAAA TCCTTGCTGG TCTGTGTGTG ATGAGTGCAG CGGCCATCTA CACAGTGAGA 
CACAGTGAGT GGCATGTCAA CAACGACTAC TCCTATGGCT TTGCTTACAT CCTGGCCTGG 
GTGGCTTTCC CGCTGGCCCT CCTTAGTGGC ATCATCTACG TGATCCTGCG GAAACGCGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A dual role for Integrin ?6?4 in modulating Hereditary Neuropathy with liability to Pressure Palsies.
Journal of neurochemistry(2018 Jan)
Poitelon Y,Matafora V,Silvestri N,Zambroni D,McGarry C,Serghany N,Rush T,Vizzuso D,Court FA,Bachi A,Wrabetz L,Feltri ML

Pmp22 mutant allele-specific siRNA alleviates demyelinating neuropathic phenotype in vivo.
Neurobiology of disease10099-107(2017 Apr)
Lee JS,Chang EH,Koo OJ,Jwa DH,Mo WM,Kwak G,Moon HW,Park HT,Hong YB,Choi BO

Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP.
PLoS genetics12(9)e1006290(2016 Sep)
Hu B,Arpag S,Zhang X,M?bius W,Werner H,Sosinsky G,Ellisman M,Zhang Y,Hamilton A,Chernoff J,Li J

Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.
Proceedings of the National Academy of Sciences of the United States of America111(50)18025-30(2014 Dec)
Lim H,Sharoukhov D,Kassim I,Zhang Y,Salzer JL,Melendez-Vasquez CV

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Pmp22 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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