Myo5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo5a
Entrez Gene ID	25017
Full Name	myosin VA
Synonyms	D,Dop,Myh12
General protein information	Preferred Names myosin VA Names unconventional myosin-Va dilute myosin heavy chain, non-muscle dilute-opisthotonus myosin 5a myosin-Va
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	may play a role in secretory granule transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022178.1	NP_071514.1	unconventional myosin-Va

Pathways from BioSystems

Homologies

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Gene Ontology
Bibliography

Related articles in PubMed

Pleiotropic neuropathological and biochemical alterations associated with Myo5a mutation in a rat Model.
Landrock KK, Sullivan P, Martini-Stoica H, Goldstein DS, Graham BH, Yamamoto S, Bellen HJ, Gibbs RA, Chen R, D'Amelio M, Stoica G
Brain research1679155-170(2018 Jan)

MST3 kinase phosphorylates TAO1/2 to enable Myosin Va function in promoting spine synapse development.
Ultanir SK, Yadav S, Hertz NT, Oses-Prieto JA, Claxton S, Burlingame AL, Shokat KM, Jan LY, Jan YN
Neuron84(5)968-82(2014 Dec)

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
DiTommaso T, Jones LK, Cottle DL, , Gerdin AK, Vancollie VE, Watt FM, Ramirez-Solis R, Bradley A, Steel KP, Sundberg JP, White JK, Smyth IM
PLoS genetics10(10)e1004705(2014 Oct)

The high molecular weight neurofilament subunit plays an essential role in axonal outgrowth and stabilization.
Lee S, Shea TB
Biology open3(10)974-81(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Insulin signaling to the molecular switch Rab8A connects with the motor protein MyoVa to mobilize GLUT4 vesicles toward the muscle cell plasma membrane.
Title: Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells.

Data demonstrate that myosin-Va is a component of a heterogeneous population of ribonucleoprotein particles of different sizes and composition, both in brain and peripheral nervous system
Title: Myosin Va associates with mRNA in ribonucleoprotein particles present in myelinated peripheral axons and in the central nervous system.

targeting of mRNAs to the dendrites is mediated by a mechanism that is dependent on actin and Myosin Va.
Title: Actin and myosin-dependent localization of mRNA to dendrites.

Myosin-Va mediates cell-to-cell transfer of RNA from Schwann cells to axons.
Title: Myosin-Va-dependent cell-to-cell transfer of RNA from Schwann cells to axons.

Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a.
Title: Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a.

The following Myo5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05363	NM_022178.1 Latest version!	Rattus norvegicus myosin VA (Myo5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo5a ( NM_022178.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071514.1 Rattus norvegicus myosin VA (Myo5a), mRNA.

Price & Availability↑

CloneID	ORa05363
Clone ID Related Accession (Same CDS sequence)	NM_022178.1
Accession Version	NM_022178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	unconventional myosin-Va
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB035736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035736.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTTGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGATC CAAAGACCAG CGAGCTCCCT 
CACCTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCTG CTGTGCTGCA CAATCTCCGC GTCCGCTTCA TCGACTCCAA ACTCATTTAT 
ACGTATTGTG GGATAGTCCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATCTATGGA 
GAAGATATCA TTAATGCATA CAGTGGCCAG AACATGGGTG ACATGGACCC TCACATCTTT 
GCAGTAGCTG AAGAGGCCTA TAAACAAATG GCAAGGGACG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGTGC AGGGAAGACA GTCTCGGCTA AGTATGCCAT GAGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGGAAATATA TTGAAATTGG TTTTGACAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG CTACCTGAGT TTAAGATGCT GCGGTTAGGA 
AATGCAGATA GTTTCCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTGGAC 
GATGCGAAGG AGATGGCACA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG GATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGGTTT 
GCGTCTCGAG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAGCCTCT TATCATTTTC 
TGTGATCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTA 
GCTACCGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GACGCTTTAG CAAAGCATAT CTATGCAAAG CTCTTTAACT GGATTGTCGG CCATGTCAAT 
CAGGCTCTCC ATTCAGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAG 
CTACAGCAAC AATTCAACAT GCATGTCTTC AAACTGGAAC AGGAGGAGTA CATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GTATTCTAGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGTACCGAT 
GACACATGGG CCCAAAAACT ATACAACACA CATTTGAACA AATGTGCTCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAC ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGACGAGAA GGCCATCAGT 
CCCACCTCTG CCACTTCCTC AGGACGCACA CCTCTCACAC GGGTACCTGT AAAGCCCACC 
AAGGGTCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACT TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AACGCCACTA CTCCTCACTA TGTTCGCTGT 
ATTAAGCCCA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGAGC AGTACAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCAAGG 
TGGACTTATC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA GGATGTGCTG 
GGAGATAGAA AGCAAACATG CCAGAATGTA TTAGAGAAAC TGATACTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTT CGTGCTGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGGGCTG ACAAACTGAG GGCGGCCTGC ATCCGGATCC AGAAGACGAT TCGTGGGTGG 
CTGCTGAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGTGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAGAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAAGT ACAAGATTAG ACGAGCTGCC 
ACCATTGTTC TTCAGTCTTA TTTGAGAGGC TACTTGGCAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCTCGTACA 
CATTATAAGA GGACCATGAA AGCCATCATC TACCTTCAAT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAAAA CAAGATTATG CAGCTGCAAC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GCGAGGAGGA AGCTAAGGTT 
GCCACTGGGA GAGTGCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAG 
CAAACTCGAT CGGAGAAAAA GTCCATTGAA GAACGAGCAG ATAAATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAACTT GAAGGAAGAA AACACTCTGC TGAAGCAGGA AAAGGAGACC 
CTCAATCACC TCATGGTGGA GCAGGCTAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAGCTCGAC CTGAATGATG AGAGGCTGAG GTATCAAAAC 
CTCCTGAATG AGTTCAGTCG TCTGGAGGAG CGCTATGACG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTCAG CTCAGAATTT GCAGAAACTG AAGACATTGC ACCAAGGACA 
GAGGAGCCAA CTGAGAAGAA GGTGCCTTTG GATATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGTGTTACAG AGCTGGGACA GGAGAAGCAG TTGATGCAGG ATGAGCTGGA CCGAAAGGAG 
GAGCAGGTGT TGCGCAGCAA GGCAAAGGGA GGAGAAAGAC CACAGATTAG AGGTGCTGAA 
CTGGGATATG AGTCCCTCAA GCGTCAGGAA CTGGAGTCAG AAAACAAAAA GCTGAAGAAT 
GAGCTGAATG AGCTACGCAA AGCCCTGAGT GAGAAGAGTG CCCCCGAAGT GAATGCGCCA 
GGCGCGCCCG CTTACCGCGT CCTCATGGAG CAGCTGACGG CCGTGAGCGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATTTTAAGG TCTCAGCTGG TGAGCCAGAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACGAACAGG 
CTCTTAGAAT CCCAGCTACA GTCGCAGAAG AGGAGCCATG AAAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACCGTCCGG 
AAACTGAAGA AACAACTAAA AGTCTTTGCC AAAAAAATCG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATC GATGAGCCTA TCCGGCCGGT CAACATTCCC 
CGGAAAGGAA AGGATTTCCA AGGGATGCTG GAGTACAAGC GGGAGGACGA ACAGAAGCTT 
GTTAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTTCAGGG 
TTACCGGCAT ATATCTTGTT TATGTGTGTG CGACATGCCG ACTACCTGGA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCCT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA AGAGGGCTTC ATGAAACACA ACACGTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTTGCTGAG TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGCATGCTAG AGCATGAAAC AATTCAGGGA GTATCTGGGG TGAAGCCCAC AGGACTCAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCATTCCGT CATGTGTCAG CATGGCATGG ACCCAGAGCT AATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGC GCCATCACCC TGAACAACCT CCTGCTGCGC 
AAGGACATGT GCTCCTGGAG CAAAGGCATG CAGATCAGGT ACAATGTCAG TCAATTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCAA AAGAGACTCT GGAACCTCTT 
ATCCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC GGAAGCCATC 
TGCTCCATGT GCAACGCTCT GACCACAGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCCGTTAATG AGTTTGAGGA AAGGGTCTCT GTTTCATTTA TCCGCACTAT ACAGGTGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTCCCCT TTAACCCATC CTCCCTGGCC CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGCT TCATTGCAAG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071514.1
CDS	41..5527
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTSELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLIIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVGHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGLQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCQNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRKYKIRRAATIVLQSYLRGYLARNRYRKILREHKAVIIQKRVRGWLARTHYKRTMKAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETEQLVSNLKEENTLLKQEKETLNHLMVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPTEKKVPLDMSLFLKLQKRVTELGQEKQLMQDELDRKEEQVLRSKAKGGERPQIRGAELGYESLKRQELESENKKLKNELNELRKALSEKSAPEVNAPGAPAYRVLMEQLTAVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKGKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLISGLPAYILFMCVRHADYLDDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQVRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	NM_022178.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin VA (Myo5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022178.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTTGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGATC CAAAGACCAG CGAGCTCCCT 
CACCTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCTG CTGTGCTGCA CAATCTCCGC GTCCGCTTCA TCGACTCCAA ACTCATTTAT 
ACGTATTGTG GGATAGTCCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATCTATGGA 
GAAGATATCA TTAATGCATA CAGTGGCCAG AACATGGGTG ACATGGACCC TCACATCTTT 
GCAGTAGCTG AAGAGGCCTA TAAACAAATG GCAAGGGACG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGTGC AGGGAAGACA GTCTCGGCTA AGTATGCCAT GAGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGGAAATATA TTGAAATTGG TTTTGACAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG CTACCTGAGT TTAAGATGCT GCGGTTAGGA 
AATGCAGATA GTTTCCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTGGAC 
GATGCGAAGG AGATGGCACA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG GATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGGTTT 
GCGTCTCGAG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAGCCTCT TATCATTTTC 
TGTGATCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTA 
GCTACCGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GACGCTTTAG CAAAGCATAT CTATGCAAAG CTCTTTAACT GGATTGTCGG CCATGTCAAT 
CAGGCTCTCC ATTCAGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAG 
CTACAGCAAC AATTCAACAT GCATGTCTTC AAACTGGAAC AGGAGGAGTA CATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GTATTCTAGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGTACCGAT 
GACACATGGG CCCAAAAACT ATACAACACA CATTTGAACA AATGTGCTCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAC ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGACGAGAA GGCCATCAGT 
CCCACCTCTG CCACTTCCTC AGGACGCACA CCTCTCACAC GGGTACCTGT AAAGCCCACC 
AAGGGTCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACT TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AACGCCACTA CTCCTCACTA TGTTCGCTGT 
ATTAAGCCCA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGAGC AGTACAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCAAGG 
TGGACTTATC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA GGATGTGCTG 
GGAGATAGAA AGCAAACATG CCAGAATGTA TTAGAGAAAC TGATACTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTT CGTGCTGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGGGCTG ACAAACTGAG GGCGGCCTGC ATCCGGATCC AGAAGACGAT TCGTGGGTGG 
CTGCTGAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGTGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAGAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAAGT ACAAGATTAG ACGAGCTGCC 
ACCATTGTTC TTCAGTCTTA TTTGAGAGGC TACTTGGCAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCTCGTACA 
CATTATAAGA GGACCATGAA AGCCATCATC TACCTTCAAT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAAAA CAAGATTATG CAGCTGCAAC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GCGAGGAGGA AGCTAAGGTT 
GCCACTGGGA GAGTGCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAG 
CAAACTCGAT CGGAGAAAAA GTCCATTGAA GAACGAGCAG ATAAATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAACTT GAAGGAAGAA AACACTCTGC TGAAGCAGGA AAAGGAGACC 
CTCAATCACC TCATGGTGGA GCAGGCTAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAGCTCGAC CTGAATGATG AGAGGCTGAG GTATCAAAAC 
CTCCTGAATG AGTTCAGTCG TCTGGAGGAG CGCTATGACG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTCAG CTCAGAATTT GCAGAAACTG AAGACATTGC ACCAAGGACA 
GAGGAGCCAA CTGAGAAGAA GGTGCCTTTG GATATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGTGTTACAG AGCTGGGACA GGAGAAGCAG TTGATGCAGG ATGAGCTGGA CCGAAAGGAG 
GAGCAGGTGT TGCGCAGCAA GGCAAAGGGA GGAGAAAGAC CACAGATTAG AGGTGCTGAA 
CTGGGATATG AGTCCCTCAA GCGTCAGGAA CTGGAGTCAG AAAACAAAAA GCTGAAGAAT 
GAGCTGAATG AGCTACGCAA AGCCCTGAGT GAGAAGAGTG CCCCCGAAGT GAATGCGCCA 
GGCGCGCCCG CTTACCGCGT CCTCATGGAG CAGCTGACGG CCGTGAGCGA GGAGCTCGAT 
GTCCGCAAGG AGGAAGTCCT CATTTTAAGG TCTCAGCTGG TGAGCCAGAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACGAACAGG 
CTCTTAGAAT CCCAGCTACA GTCGCAGAAG AGGAGCCATG AAAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACCGTCCGG 
AAACTGAAGA AACAACTAAA AGTCTTTGCC AAAAAAATCG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATC GATGAGCCTA TCCGGCCGGT CAACATTCCC 
CGGAAAGGAA AGGATTTCCA AGGGATGCTG GAGTACAAGC GGGAGGACGA ACAGAAGCTT 
GTTAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTTCAGGG 
TTACCGGCAT ATATCTTGTT TATGTGTGTG CGACATGCCG ACTACCTGGA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCCT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA AGAGGGCTTC ATGAAACACA ACACGTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTTGCTGAG TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGCATGCTAG AGCATGAAAC AATTCAGGGA GTATCTGGGG TGAAGCCCAC AGGACTCAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTGCGGCAG 
CTCAACTCCT TCCATTCCGT CATGTGTCAG CATGGCATGG ACCCAGAGCT AATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCGTGGGC GCCATCACCC TGAACAACCT CCTGCTGCGC 
AAGGACATGT GCTCCTGGAG CAAAGGCATG CAGATCAGGT ACAATGTCAG TCAATTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCAA AAGAGACTCT GGAACCTCTT 
ATCCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC GGAAGCCATC 
TGCTCCATGT GCAACGCTCT GACCACAGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCCGTTAATG AGTTTGAGGA AAGGGTCTCT GTTTCATTTA TCCGCACTAT ACAGGTGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTCCCCT TTAACCCATC CTCCCTGGCC CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGCT TCATTGCAAG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pleiotropic neuropathological and biochemical alterations associated with Myo5a mutation in a rat Model.
Brain research1679155-170(2018 Jan)
Landrock KK,Sullivan P,Martini-Stoica H,Goldstein DS,Graham BH,Yamamoto S,Bellen HJ,Gibbs RA,Chen R,D'Amelio M,Stoica G

MST3 kinase phosphorylates TAO1/2 to enable Myosin Va function in promoting spine synapse development.
Neuron84(5)968-82(2014 Dec)
Ultanir SK,Yadav S,Hertz NT,Oses-Prieto JA,Claxton S,Burlingame AL,Shokat KM,Jan LY,Jan YN

Identification of genes important for cutaneous function revealed by a large scale reverse genetic screen in the mouse.
PLoS genetics10(10)e1004705(2014 Oct)
DiTommaso T,Jones LK,Cottle DL,,Gerdin AK,Vancollie VE,Watt FM,Ramirez-Solis R,Bradley A,Steel KP,Sundberg JP,White JK,Smyth IM

The high molecular weight neurofilament subunit plays an essential role in axonal outgrowth and stabilization.
Biology open3(10)974-81(2014 Sep)
Lee S,Shea TB

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Myo5a cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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