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Nkx2-1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Nkx2-1
Entrez Gene ID 25628
Full Name NK2 homeobox 1
Synonyms TTF1,Titf1
General protein information
Preferred Names

NK2 homeobox 1

Names

homeobox protein Nkx-2.1
TTF-1
thyroid nuclear factor 1
thyroid transcription factor 1 TTF-1 NK-2
thyroid-specific transcription factor 1
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

6

6q23
Summary transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor; may play a role in organogenesis and/or reproductive maturation and development [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006240118.2 XP_006240180.1 homeobox protein Nkx-2.1 isoform X3
XM_006240116.2 XP_006240178.1 homeobox protein Nkx-2.1 isoform X1
XM_006240117.3 XP_006240179.1 homeobox protein Nkx-2.1 isoform X2
NM_013093.1 NP_037225.1 homeobox protein Nkx-2.1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Nkx2-1 NP_037225.1
Danio rerio (zebrafish) nkx2.1b NP_571851.1
Xenopus tropicalis (tropical clawed frog) nkx2-1 XP_002935383.1
Homo sapiens (human) NKX2-1 NP_001073136.1
Pan troglodytes (chimpanzee) NKX2-1 XP_509911.2
NKX2-1 XP_001089773.1
Bos taurus (cattle) LOC613713 NP_001178387.1
Mus musculus (house mouse) Nkx2-1 NP_001139670.1
Gallus gallus (chicken) NKX2-1 NP_989947.1

Gene Ontology


Bibliography

Related articles in PubMed

Adipogenic Differentiation of Thyroid Cancer Cells Through the Pax8-PPAR? Fusion Protein Is Regulated by Thyroid Transcription Factor 1 (TTF-1).
Xu B, O'Donnell M, O'Donnell J, Yu J, Zhang Y, Sartor MA, Koenig RJ
The Journal of biological chemistry291(37)19274-86(2016 09)

Benign hereditary chorea, not only chorea: a family case presentation.
Koht J, L?stegaard SO, Wedding I, Vidailhet M, Louha M, Tallaksen CM
Cerebellum & ataxias33(2016)

Gene expression, function, and diversity of Nkx2-4 in the rainbow trout, Oncorhynchus mykiss.
Uemae Y, Sakamoto J, Hidaka Y, Hiratsuka A, Susa T, Kato Y, Suzuki M
General and comparative endocrinology206193-202(2014 Sep)

Molecular cloning and functional characterization of two forms of Pax8 in the rainbow trout, Oncorhynchus mykiss.
Katagiri N, Uemae Y, Sakamoto J, Hidaka Y, Susa T, Kato Y, Kimura S, Suzuki M
General and comparative endocrinology19822-31(2014 Mar)

NKX2-1 mutation in a family diagnosed with ataxic dyskinetic cerebral palsy.
McMichael G, Haan E, Gardner A, Yap TY, Thompson S, Ouvrier R, Dale RC, Gecz J, Maclennan AH
European journal of medical genetics56(9)506-9(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that TTF-1 interacts with PPFP to inhibit the pro-adipogenic response to pioglitazone, and that the ability of pioglitazone to decrease TTF-1 expression contributes to its pro-adipogenic action.
Title: Adipogenic Differentiation of Thyroid Cancer Cells Through the Pax8-PPAR? Fusion Protein Is Regulated by Thyroid Transcription Factor 1 (TTF-1).

Dual positive effects of leptin were found on protein expression and transcriptional activity of thyroid transcription factor-1
Title: Leptin promotes fetal lung maturity and upregulates SP-A expression in pulmonary alveoli type-II epithelial cells involving TTF-1 activation.

TTF1 contributes to regulating periodic oscillations in expression of the gonadotrophin-releasing hormone (GnRH) gene expression; TTF1 transactivates GnRH transcription by binding to two sites in the GnRH promoter.
Title: Thyroid transcription factor 1, a homeodomain containing transcription factor, contributes to regulating periodic oscillations in GnRH gene expression.

TTF-1 transcriptionally regulates synthesis of AgRP and alpha-MSH in the ARC and affects feeding behavior via the melanocortin pathway.
Title: Thyroid transcription factor-1 regulates feeding behavior via melanocortin pathway in the hypothalamus.

The expression of Nkx2.1 mRNA in offspring of hypothyroid pregnant rat was reduced. Treatment of pregnant rats with thyroxine restored Nkx2.1 expression to nomal levels in offspring.
Title: [Effects of supplement of thyroxine for hypothyroid pregnant rat on the expression of homeobox gene Nkx2.1 mRNA in the offspring's cerebrum tissue].

The following Nkx2-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx2-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12246 NM_013093.1
Latest version!		 Rattus norvegicus NK2 homeobox 1 (Nkx2-1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
ORa12246 XM_006240118.2
Latest version!		 Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
ORa29353 XM_006240116.2
Latest version!		 Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
ORa29354 XM_006240117.3
Latest version!		 Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12246
Clone ID Related Accession (Same CDS sequence) XM_006240118.2 , NM_013093.1
Accession Version NM_013093.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-04
Organism Rattus norvegicus(Norway rat)
Product homeobox protein Nkx-2.1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07064373.1. On or before Nov 23, 2008 this sequence version replaced XM_216720.4, XM_001079296.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB894119.1, X53858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## 

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721
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841
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961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT 
TACAGACAGG GCCAGGCGGC CCCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACG TGAGCGAGCT GCCACCTTAC 
CAGGACACCA TGCGGAACAG CGCTTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGATCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAATAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC CAGCGCACCC 
CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG GCGCAGGTGT ACGAGCTCGA GCGACGCTTC 
AAGCAGCAGA AGTACCTGTC GGCGCCGGAG CGCGAGCATC TGGCCAGCAT GATTCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAGGCG 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG AGGCGGCGGC 
GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC 
GTGCCGGTCC TGGTGAAAGA CGGCAAACCC TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC 
GCCAGCCTGC AAGGCCACGC GCAACAACAA GCTCAGCAGC AGGCGCAGGC GGCACAAGCG 
GCTGCCGCGG CCATCTCTGT GGGCAGCGGT GGTGCGGGTC TAGGAGCACA CCCAGGCCAC 
CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC CTGGCGCACC ACGCAGCCAG CCCCGCAGCG 
CTGCAGGGCC AGGTCTCTAG CCTATCTCAT CTGAACTCCT CTGGCTCGGA CTATGGCGCC 
ATGTCTTGCT CTACTTTGCT TTATGGTCGG ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_037225.1
CDS1..1119
Translation

Target ORF information:

RefSeq Version NM_013093.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NK2 homeobox 1 (Nkx2-1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_013093.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT 
TACAGACAGG GCCAGGCGGC CCCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACG TGAGCGAGCT GCCACCTTAC 
CAGGACACCA TGCGGAACAG CGCTTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGATCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAATAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC CAGCGCACCC 
CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG GCGCAGGTGT ACGAGCTCGA GCGACGCTTC 
AAGCAGCAGA AGTACCTGTC GGCGCCGGAG CGCGAGCATC TGGCCAGCAT GATTCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAGGCG 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG AGGCGGCGGC 
GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC 
GTGCCGGTCC TGGTGAAAGA CGGCAAACCC TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC 
GCCAGCCTGC AAGGCCACGC GCAACAACAA GCTCAGCAGC AGGCGCAGGC GGCACAAGCG 
GCTGCCGCGG CCATCTCTGT GGGCAGCGGT GGTGCGGGTC TAGGAGCACA CCCAGGCCAC 
CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC CTGGCGCACC ACGCAGCCAG CCCCGCAGCG 
CTGCAGGGCC AGGTCTCTAG CCTATCTCAT CTGAACTCCT CTGGCTCGGA CTATGGCGCC 
ATGTCTTGCT CTACTTTGCT TTATGGTCGG ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa12246
Clone ID Related Accession (Same CDS sequence) XM_006240118.2 , NM_013093.1
Accession Version XM_006240118.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product homeobox protein Nkx-2.1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT 
TACAGACAGG GCCAGGCGGC CCCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACG TGAGCGAGCT GCCACCTTAC 
CAGGACACCA TGCGGAACAG CGCTTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGATCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAATAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC CAGCGCACCC 
CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG GCGCAGGTGT ACGAGCTCGA GCGACGCTTC 
AAGCAGCAGA AGTACCTGTC GGCGCCGGAG CGCGAGCATC TGGCCAGCAT GATTCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAGGCG 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG AGGCGGCGGC 
GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC 
GTGCCGGTCC TGGTGAAAGA CGGCAAACCC TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC 
GCCAGCCTGC AAGGCCACGC GCAACAACAA GCTCAGCAGC AGGCGCAGGC GGCACAAGCG 
GCTGCCGCGG CCATCTCTGT GGGCAGCGGT GGTGCGGGTC TAGGAGCACA CCCAGGCCAC 
CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC CTGGCGCACC ACGCAGCCAG CCCCGCAGCG 
CTGCAGGGCC AGGTCTCTAG CCTATCTCAT CTGAACTCCT CTGGCTCGGA CTATGGCGCC 
ATGTCTTGCT CTACTTTGCT TTATGGTCGG ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006240180.1
CDS866..1984
Translation

Target ORF information:

RefSeq Version XM_006240118.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006240118.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT 
TACAGACAGG GCCAGGCGGC CCCGCCGGCC GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACG TGAGCGAGCT GCCACCTTAC 
CAGGACACCA TGCGGAACAG CGCTTCGGGC CCCGGATGGT ACGGCGCCAA CCCAGATCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAATAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC CAGCGCACCC 
CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG GCGCAGGTGT ACGAGCTCGA GCGACGCTTC 
AAGCAGCAGA AGTACCTGTC GGCGCCGGAG CGCGAGCATC TGGCCAGCAT GATTCACCTG 
ACACCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAGATGAA GCGCCAGGCG 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG AGGCGGCGGC 
GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC 
GTGCCGGTCC TGGTGAAAGA CGGCAAACCC TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC 
GCCAGCCTGC AAGGCCACGC GCAACAACAA GCTCAGCAGC AGGCGCAGGC GGCACAAGCG 
GCTGCCGCGG CCATCTCTGT GGGCAGCGGT GGTGCGGGTC TAGGAGCACA CCCAGGCCAC 
CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC CTGGCGCACC ACGCAGCCAG CCCCGCAGCG 
CTGCAGGGCC AGGTCTCTAG CCTATCTCAT CTGAACTCCT CTGGCTCGGA CTATGGCGCC 
ATGTCTTGCT CTACTTTGCT TTATGGTCGG ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa29353
Clone ID Related Accession (Same CDS sequence) XM_006240116.2
Accession Version XM_006240116.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product homeobox protein Nkx-2.1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCGCC TCTGGTGGCT GCCTAAAACC TGGCGCCGGG CTAAAACAAA CGCGAGGCAG 
CCCCCGAGCC TCCACTCAAG CCAATTAAGG CGGACTCGGT CCACTCCGTT ACGTGTACAT 
CCAACAAGAT CGGCGTTAAG CCGACGCCGA ATCATGTCGA TGAGTCCAAA GCACACGACT 
CCGTTCTCAG TGTCTGACAT CTTGAGTCCC CTGGAGGAAA GCTACAAGAA AGTGGGCATG 
GAGGGCGGCG GCCTCGGGGC TCCGCTCGCA GCTTACAGAC AGGGCCAGGC GGCCCCGCCG 
GCCGCGGCCA TGCAGCAGCA CGCCGTGGGG CACCACGGCG CCGTCACCGC CGCCTACCAC 
ATGACGGCGG CGGGGGTGCC CCAGCTCTCC CACTCCGCCG TGGGGGGCTA CTGCAACGGC 
AACCTGGGCA ACGTGAGCGA GCTGCCACCT TACCAGGACA CCATGCGGAA CAGCGCTTCG 
GGCCCCGGAT GGTACGGCGC CAACCCAGAT CCGCGCTTCC CCGCCATCTC CCGCTTCATG 
GGCCCGGCGA GCGGCATGAA TATGAGCGGC ATGGGCGGCC TGGGCTCGCT GGGGGACGTG 
AGCAAGAACA TGGCCCCGCT GCCCAGCGCA CCCCGCCGGA AGCGTCGGGT GCTCTTCTCC 
CAGGCGCAGG TGTACGAGCT CGAGCGACGC TTCAAGCAGC AGAAGTACCT GTCGGCGCCG 
GAGCGCGAGC ATCTGGCCAG CATGATTCAC CTGACACCCA CGCAGGTCAA GATCTGGTTC 
CAGAACCACC GCTACAAGAT GAAGCGCCAG GCGAAGGACA AGGCGGCGCA GCAGCAACTG 
CAGCAGGACA GCGGCGGCGG CGGAGGCGGC GGCGGCGGCG CGGGTTGCCC GCAGCAGCAG 
CAAGCTCAGC AGCAGTCGCC GCGCCGGGTG GCCGTGCCGG TCCTGGTGAA AGACGGCAAA 
CCCTGCCAAG CGGGCGCCCC TGCACCGGGA GCCGCCAGCC TGCAAGGCCA CGCGCAACAA 
CAAGCTCAGC AGCAGGCGCA GGCGGCACAA GCGGCTGCCG CGGCCATCTC TGTGGGCAGC 
GGTGGTGCGG GTCTAGGAGC ACACCCAGGC CACCAGCCCG GCAGCGCAGG GCAGTCCCCG 
GACCTGGCGC ACCACGCAGC CAGCCCCGCA GCGCTGCAGG GCCAGGTCTC TAGCCTATCT 
CATCTGAACT CCTCTGGCTC GGACTATGGC GCCATGTCTT GCTCTACTTT GCTTTATGGT 
CGGACCTGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006240178.1
CDS129..1400
Translation

Target ORF information:

RefSeq Version XM_006240116.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006240116.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCGCC TCTGGTGGCT GCCTAAAACC TGGCGCCGGG CTAAAACAAA CGCGAGGCAG 
CCCCCGAGCC TCCACTCAAG CCAATTAAGG CGGACTCGGT CCACTCCGTT ACGTGTACAT 
CCAACAAGAT CGGCGTTAAG CCGACGCCGA ATCATGTCGA TGAGTCCAAA GCACACGACT 
CCGTTCTCAG TGTCTGACAT CTTGAGTCCC CTGGAGGAAA GCTACAAGAA AGTGGGCATG 
GAGGGCGGCG GCCTCGGGGC TCCGCTCGCA GCTTACAGAC AGGGCCAGGC GGCCCCGCCG 
GCCGCGGCCA TGCAGCAGCA CGCCGTGGGG CACCACGGCG CCGTCACCGC CGCCTACCAC 
ATGACGGCGG CGGGGGTGCC CCAGCTCTCC CACTCCGCCG TGGGGGGCTA CTGCAACGGC 
AACCTGGGCA ACGTGAGCGA GCTGCCACCT TACCAGGACA CCATGCGGAA CAGCGCTTCG 
GGCCCCGGAT GGTACGGCGC CAACCCAGAT CCGCGCTTCC CCGCCATCTC CCGCTTCATG 
GGCCCGGCGA GCGGCATGAA TATGAGCGGC ATGGGCGGCC TGGGCTCGCT GGGGGACGTG 
AGCAAGAACA TGGCCCCGCT GCCCAGCGCA CCCCGCCGGA AGCGTCGGGT GCTCTTCTCC 
CAGGCGCAGG TGTACGAGCT CGAGCGACGC TTCAAGCAGC AGAAGTACCT GTCGGCGCCG 
GAGCGCGAGC ATCTGGCCAG CATGATTCAC CTGACACCCA CGCAGGTCAA GATCTGGTTC 
CAGAACCACC GCTACAAGAT GAAGCGCCAG GCGAAGGACA AGGCGGCGCA GCAGCAACTG 
CAGCAGGACA GCGGCGGCGG CGGAGGCGGC GGCGGCGGCG CGGGTTGCCC GCAGCAGCAG 
CAAGCTCAGC AGCAGTCGCC GCGCCGGGTG GCCGTGCCGG TCCTGGTGAA AGACGGCAAA 
CCCTGCCAAG CGGGCGCCCC TGCACCGGGA GCCGCCAGCC TGCAAGGCCA CGCGCAACAA 
CAAGCTCAGC AGCAGGCGCA GGCGGCACAA GCGGCTGCCG CGGCCATCTC TGTGGGCAGC 
GGTGGTGCGG GTCTAGGAGC ACACCCAGGC CACCAGCCCG GCAGCGCAGG GCAGTCCCCG 
GACCTGGCGC ACCACGCAGC CAGCCCCGCA GCGCTGCAGG GCCAGGTCTC TAGCCTATCT 
CATCTGAACT CCTCTGGCTC GGACTATGGC GCCATGTCTT GCTCTACTTT GCTTTATGGT 
CGGACCTGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa29354
Clone ID Related Accession (Same CDS sequence) XM_006240117.3
Accession Version XM_006240117.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1209bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product homeobox protein Nkx-2.1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCGGTGG GAAGGCGCGG GGCTGGGAGG CCGCAGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG ACGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT TACAGACAGG GCCAGGCGGC CCCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACG TGAGCGAGCT GCCACCTTAC CAGGACACCA TGCGGAACAG CGCTTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGATCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAATAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC CAGCGCACCC CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ACGAGCTCGA GCGACGCTTC AAGCAGCAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCATC TGGCCAGCAT GATTCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAGGCG AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG AGGCGGCGGC GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA 
GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC GTGCCGGTCC TGGTGAAAGA CGGCAAACCC 
TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC GCCAGCCTGC AAGGCCACGC GCAACAACAA 
GCTCAGCAGC AGGCGCAGGC GGCACAAGCG GCTGCCGCGG CCATCTCTGT GGGCAGCGGT 
GGTGCGGGTC TAGGAGCACA CCCAGGCCAC CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC 
CTGGCGCACC ACGCAGCCAG CCCCGCAGCG CTGCAGGGCC AGGTCTCTAG CCTATCTCAT 
CTGAACTCCT CTGGCTCGGA CTATGGCGCC ATGTCTTGCT CTACTTTGCT TTATGGTCGG 
ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006240179.1
CDS272..1480
Translation

Target ORF information:

RefSeq Version XM_006240117.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NK2 homeobox 1 (Nkx2-1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006240117.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCGGTGG GAAGGCGCGG GGCTGGGAGG CCGCAGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG ACGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTCGCAGCT TACAGACAGG GCCAGGCGGC CCCGCCGGCC 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCCCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACG TGAGCGAGCT GCCACCTTAC CAGGACACCA TGCGGAACAG CGCTTCGGGC 
CCCGGATGGT ACGGCGCCAA CCCAGATCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAATAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC CAGCGCACCC CGCCGGAAGC GTCGGGTGCT CTTCTCCCAG 
GCGCAGGTGT ACGAGCTCGA GCGACGCTTC AAGCAGCAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCATC TGGCCAGCAT GATTCACCTG ACACCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAGATGAA GCGCCAGGCG AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG AGGCGGCGGC GGCGGCGCGG GTTGCCCGCA GCAGCAGCAA 
GCTCAGCAGC AGTCGCCGCG CCGGGTGGCC GTGCCGGTCC TGGTGAAAGA CGGCAAACCC 
TGCCAAGCGG GCGCCCCTGC ACCGGGAGCC GCCAGCCTGC AAGGCCACGC GCAACAACAA 
GCTCAGCAGC AGGCGCAGGC GGCACAAGCG GCTGCCGCGG CCATCTCTGT GGGCAGCGGT 
GGTGCGGGTC TAGGAGCACA CCCAGGCCAC CAGCCCGGCA GCGCAGGGCA GTCCCCGGAC 
CTGGCGCACC ACGCAGCCAG CCCCGCAGCG CTGCAGGGCC AGGTCTCTAG CCTATCTCAT 
CTGAACTCCT CTGGCTCGGA CTATGGCGCC ATGTCTTGCT CTACTTTGCT TTATGGTCGG 
ACCTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

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Xu B,O'Donnell M,O'Donnell J,Yu J,Zhang Y,Sartor MA,Koenig RJ


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Benign hereditary chorea, not only chorea: a family case presentation.
Cerebellum & ataxias33(2016)
Koht J,L?stegaard SO,Wedding I,Vidailhet M,Louha M,Tallaksen CM


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Gene expression, function, and diversity of Nkx2-4 in the rainbow trout, Oncorhynchus mykiss.
General and comparative endocrinology206193-202(2014 Sep)
Uemae Y,Sakamoto J,Hidaka Y,Hiratsuka A,Susa T,Kato Y,Suzuki M


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Molecular cloning and functional characterization of two forms of Pax8 in the rainbow trout, Oncorhynchus mykiss.
General and comparative endocrinology19822-31(2014 Mar)
Katagiri N,Uemae Y,Sakamoto J,Hidaka Y,Susa T,Kato Y,Kimura S,Suzuki M


book

NKX2-1 mutation in a family diagnosed with ataxic dyskinetic cerebral palsy.
European journal of medical genetics56(9)506-9(2013 Sep)
McMichael G,Haan E,Gardner A,Yap TY,Thompson S,Ouvrier R,Dale RC,Gecz J,Maclennan AH