Rims4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rims4
Entrez Gene ID	266976
Full Name	regulating synaptic membrane exocytosis 4
Synonyms	Rim4gamma
General protein information	Preferred Names regulating synaptic membrane exocytosis 4 Names regulating synaptic membrane exocytosis protein 4 RIM 4 RIM4 gamma rab3-interacting molecule 4 synaptic regulatory protein RIM4gamma
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	member of a family of synaptic proteins involved in neurotransmitter release [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762471.2	XP_008760693.1	regulating synaptic membrane exocytosis protein 4 isoform X1
NM_170666.1	NP_733766.1	regulating synaptic membrane exocytosis protein 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RIMS4	XP_003433260.1
Bos taurus (cattle)	RIMS4	XP_002692374.2
Rattus norvegicus (Norway rat)	Rims4	NP_733766.1
Danio rerio (zebrafish)	LOC570923	XP_005166329.1
Pan troglodytes (chimpanzee)	RIMS4	XP_001144803.2
Homo sapiens (human)	RIMS4	NP_001192246.1
Mus musculus (house mouse)	Rims4	NP_898844.1
Xenopus tropicalis (tropical clawed frog)	rims4	XP_004918712.1
	RIMS4	XP_001109689.1
Gallus gallus (chicken)	RIMS4	XP_425700.2

Gene Ontology
Bibliography

Related articles in PubMed

RIM3? and RIM4? are key regulators of neuronal arborization.
Alvarez-Baron E, Michel K, Mittelstaedt T, Opitz T, Schmitz F, Beck H, Dietrich D, Becker AJ, Schoch S
The Journal of neuroscience : the official journal of the Society for Neuroscience33(2)824-39(2013 Jan)

Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.
Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y
The Journal of biological chemistry285(28)21750-67(2010 Jul)

Genomic definition of RIM proteins: evolutionary amplification of a family of synaptic regulatory proteins.
Wang Y, S?dhof TC
Genomics81(2)126-37(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gamma-RIMs are involved in cell biological functions distinct from the regulation of synaptic vesicle exocytosis and play a role in the molecular mechanisms controlling the establishment of dendritic complexity and axonal outgrowth
Title: RIM3? and RIM4? are key regulators of neuronal arborization.

The following Rims4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rims4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03208	NM_170666.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa44519	XM_008762471.2 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rims4 ( NM_170666.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_733766.1 Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), mRNA.

Price & Availability↑

CloneID	ORa03208
Clone ID Related Accession (Same CDS sequence)	NM_170666.1
Accession Version	NM_170666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF548739.1. ##Evidence-Data-START## Transcript exon combination :: AF548739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632258 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGCT CGCAGAGCCG CCTCAGCCTG TCCGCCTCCT TCGAGGCGCT CGCCATCTAC 
TTCCCGTGCA TGAACTCCTT CGATGATGAG GACGCAGCAG ACAGCCGGAG GCTGAAGGGT 
GCCATCCAGA GAAGCACAGA GACAGGGCTG GCCGTGGAGA TGCCCAGCCG GACGTTACGC 
CAAGCCAGCC ATGAGTCCAT CGAGGACAGC ATGAATAGCT ATGGCTCAGA GGGCAACCTG 
AACTATGGAG GAGTTTGTCT AGCTTCGGAT GCCCAGTTCA GTGACTTCCT GGGGAGCATG 
GGGCCAGCAC AGTTTGTGGG CCGCCAGACC CTGGCTACCA CACCCATGGG GGGTGTGGAG 
ATCGGTTTAC AGGAGCGGAA CGGTCAGCTG GAGGTGGACA TCATCCAGGC ACGCGGGCTG 
ACTGCCAAGC CAGGCTCGAA GACACTGCCA GCTGCCTATA TCAAGGCCTA TCTGCTGGAA 
AATGGTGTCT GCATTGCCAA GAAGAAGACC AAAGTGGCCC GCAAGTCACT GGACCCATTG 
TACAACCAGG TGCTTCTGTT TCCTGAGAGT CCCCAGGGCA AAGTCCTGCA GGTAATTGTG 
TGGGGAAACT ACGGACGAAT GGAGCGGAAG CAGTTCATGG GCGTGGCTCG AGTGCTGCTG 
GAGGAACTGG ACTTGACCAC CCTGGCCGTG GGTTGGTACA AGCTCTTCCC CACCTCCTCC 
ATGGTGGACC CAGCCACAGG TCCCCTGCTT CGGCAGGCAT CCCAGCTGTC CCTCGAGAGC 
ACCGTGGGGC CCTGTGGCGA GCGATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733766.1
CDS	1..810
Translation	MERSQSRLSLSASFEALAIYFPCMNSFDDEDAADSRRLKGAIQRSTETGLAVEMPSRTLRQASHESIEDSMNSYGSEGNLNYGGVCLASDAQFSDFLGSMGPAQFVGRQTLATTPMGGVEIGLQERNGQLEVDIIQARGLTAKPGSKTLPAAYIKAYLLENGVCIAKKKTKVARKSLDPLYNQVLLFPESPQGKVLQVIVWGNYGRMERKQFMGVARVLLEELDLTTLAVGWYKLFPTSSMVDPATGPLLRQASQLSLESTVGPCGERS

Target ORF information:

RefSeq Version	NM_170666.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170666.1

ORF Insert Sequence:

ATGGAGCGCT CGCAGAGCCG CCTCAGCCTG TCCGCCTCCT TCGAGGCGCT CGCCATCTAC 
TTCCCGTGCA TGAACTCCTT CGATGATGAG GACGCAGCAG ACAGCCGGAG GCTGAAGGGT 
GCCATCCAGA GAAGCACAGA GACAGGGCTG GCCGTGGAGA TGCCCAGCCG GACGTTACGC 
CAAGCCAGCC ATGAGTCCAT CGAGGACAGC ATGAATAGCT ATGGCTCAGA GGGCAACCTG 
AACTATGGAG GAGTTTGTCT AGCTTCGGAT GCCCAGTTCA GTGACTTCCT GGGGAGCATG 
GGGCCAGCAC AGTTTGTGGG CCGCCAGACC CTGGCTACCA CACCCATGGG GGGTGTGGAG 
ATCGGTTTAC AGGAGCGGAA CGGTCAGCTG GAGGTGGACA TCATCCAGGC ACGCGGGCTG 
ACTGCCAAGC CAGGCTCGAA GACACTGCCA GCTGCCTATA TCAAGGCCTA TCTGCTGGAA 
AATGGTGTCT GCATTGCCAA GAAGAAGACC AAAGTGGCCC GCAAGTCACT GGACCCATTG 
TACAACCAGG TGCTTCTGTT TCCTGAGAGT CCCCAGGGCA AAGTCCTGCA GGTAATTGTG 
TGGGGAAACT ACGGACGAAT GGAGCGGAAG CAGTTCATGG GCGTGGCTCG AGTGCTGCTG 
GAGGAACTGG ACTTGACCAC CCTGGCCGTG GGTTGGTACA AGCTCTTCCC CACCTCCTCC 
ATGGTGGACC CAGCCACAGG TCCCCTGCTT CGGCAGGCAT CCCAGCTGTC CCTCGAGAGC 
ACCGTGGGGC CCTGTGGCGA GCGATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims4 ( XM_008762471.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760693.1 Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44519
Clone ID Related Accession (Same CDS sequence)	XM_008762471.2
Accession Version	XM_008762471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCT CGCAGAGCCG CCTCAGCCTG TCCGCCTCCT TCGAGGCGCT CGCCATCTAC 
TTCCCGTGCA TGAACTCCTT CGATGATGAG GACGCAGACA GCCGGAGGCT GAAGGGTGCC 
ATCCAGAGAA GCACAGAGAC AGGGCTGGCC GTGGAGATGC CCAGCCGGAC GTTACGCCAA 
GCCAGCCATG AGTCCATCGA GGACAGCATG AATAGCTATG GCTCAGAGGG CAACCTGAAC 
TATGGAGGAG TTTGTCTAGC TTCGGATGCC CAGTTCAGTG ACTTCCTGGG GAGCATGGGG 
CCAGCACAGT TTGTGGGCCG CCAGACCCTG GCTACCACAC CCATGGGGGA TGTGGAGATC 
GGTTTACAGG AGCGGAACGG TCAGCTGGAG GTGGACATCA TCCAGGCACG CGGGCTGACT 
GCCAAGCCAG GCTCGAAGAC ACTGCCAGCT GCCTATATCA AGGCCTATCT GCTGGAAAAT 
GGTGTCTGCA TTGCCAAGAA GAAGACCAAA GTGGCCCGCA AGTCACTGGA CCCGTTGTAC 
AACCAGGTGC TTCTGTTTCC TGAGAGTCCC CAGGGCAAAG TCCTGCAGGT AATTGTGTGG 
GGAAACTACG GACGAATGGA GCGGAAGCAG TTCATGGGCG TGGCTCGAGT GCTGCTGGAG 
GAACTGGACT TGACCACCCT GGCCGTGGGT TGGTACAAGC TCTTCCCCAC CTCCTCCATG 
GTGGACCCAG CCACAGGTCC CCTGCTTCGG CAGGCATCCC AGCTGTCCCT CGAGAGCACC 
GTGGGGCCCT GTGGCGAGCG ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760693.1
CDS	215..1021
Translation	MERSQSRLSLSASFEALAIYFPCMNSFDDEDADSRRLKGAIQRSTETGLAVEMPSRTLRQASHESIEDSMNSYGSEGNLNYGGVCLASDAQFSDFLGSMGPAQFVGRQTLATTPMGDVEIGLQERNGQLEVDIIQARGLTAKPGSKTLPAAYIKAYLLENGVCIAKKKTKVARKSLDPLYNQVLLFPESPQGKVLQVIVWGNYGRMERKQFMGVARVLLEELDLTTLAVGWYKLFPTSSMVDPATGPLLRQASQLSLESTVGPCGERS

Target ORF information:

RefSeq Version	XM_008762471.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 4 (Rims4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762471.2

ORF Insert Sequence:

ATGGAGCGCT CGCAGAGCCG CCTCAGCCTG TCCGCCTCCT TCGAGGCGCT CGCCATCTAC 
TTCCCGTGCA TGAACTCCTT CGATGATGAG GACGCAGACA GCCGGAGGCT GAAGGGTGCC 
ATCCAGAGAA GCACAGAGAC AGGGCTGGCC GTGGAGATGC CCAGCCGGAC GTTACGCCAA 
GCCAGCCATG AGTCCATCGA GGACAGCATG AATAGCTATG GCTCAGAGGG CAACCTGAAC 
TATGGAGGAG TTTGTCTAGC TTCGGATGCC CAGTTCAGTG ACTTCCTGGG GAGCATGGGG 
CCAGCACAGT TTGTGGGCCG CCAGACCCTG GCTACCACAC CCATGGGGGA TGTGGAGATC 
GGTTTACAGG AGCGGAACGG TCAGCTGGAG GTGGACATCA TCCAGGCACG CGGGCTGACT 
GCCAAGCCAG GCTCGAAGAC ACTGCCAGCT GCCTATATCA AGGCCTATCT GCTGGAAAAT 
GGTGTCTGCA TTGCCAAGAA GAAGACCAAA GTGGCCCGCA AGTCACTGGA CCCGTTGTAC 
AACCAGGTGC TTCTGTTTCC TGAGAGTCCC CAGGGCAAAG TCCTGCAGGT AATTGTGTGG 
GGAAACTACG GACGAATGGA GCGGAAGCAG TTCATGGGCG TGGCTCGAGT GCTGCTGGAG 
GAACTGGACT TGACCACCCT GGCCGTGGGT TGGTACAAGC TCTTCCCCAC CTCCTCCATG 
GTGGACCCAG CCACAGGTCC CCTGCTTCGG CAGGCATCCC AGCTGTCCCT CGAGAGCACC 
GTGGGGCCCT GTGGCGAGCG ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RIM3? and RIM4? are key regulators of neuronal arborization.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(2)824-39(2013 Jan)
Alvarez-Baron E,Michel K,Mittelstaedt T,Opitz T,Schmitz F,Beck H,Dietrich D,Becker AJ,Schoch S

Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.
The Journal of biological chemistry285(28)21750-67(2010 Jul)
Uriu Y,Kiyonaka S,Miki T,Yagi M,Akiyama S,Mori E,Nakao A,Beedle AM,Campbell KP,Wakamori M,Mori Y

Genomic definition of RIM proteins: evolutionary amplification of a family of synaptic regulatory proteins.
Genomics81(2)126-37(2003 Feb)
Wang Y,S?dhof TC