Alox12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alox12
Entrez Gene ID	287454
Full Name	arachidonate 12-lipoxygenase, 12S type
Synonyms	12-LO,12S-LOX
General protein information	Preferred Names arachidonate 12-lipoxygenase, 12S type Names arachidonate 12-lipoxygenase, 12S-type 12S-lipoxygenase lipoxin synthase 12-LO platelet 12-LOX platelet-type lipoxygenase 12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246659.3	XP_006246721.1	arachidonate 12-lipoxygenase, 12S-type isoform X1
NM_001105798.1	NP_001099268.1	arachidonate 12-lipoxygenase, 12S-type

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_08499	XP_003716006.1
	ALOX12	XP_001104192.1
Mus musculus (house mouse)	Alox12	NP_031466.2
Danio rerio (zebrafish)	alox12	NP_955912.1
Pan troglodytes (chimpanzee)	ALOX12	XP_511984.3
Canis lupus familiaris (dog)	ALOX12	XP_536613.2
Rattus norvegicus (Norway rat)	Alox12	NP_001099268.1
Homo sapiens (human)	ALOX12	NP_000688.2
Bos taurus (cattle)	ALOX12	NP_001179265.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of angiotensin II type 1 receptor blocker on 12-lipoxygenase activity and slit diaphragm protein expression in type 2 diabetic rat glomeruli.
Xu HZ, Wang WN, Zhang YY, Cheng YL, Xu ZG
Journal of nephrology29(6)775-782(2016 Dec)

Role of histone modification in 12?lipoxygenase?associated p21 gene regulation.
Cui Y, Liu N, Ma F, Sun W, Wu H, Xu Z, Yuan H
Molecular medicine reports14(4)3978-84(2016 Oct)

Hypoxic preconditioning protects rat hearts against ischemia-reperfusion injury via the arachidonate12-lipoxygenase/transient receptor potential vanilloid 1 pathway.
Lu MJ, Chen YS, Huang HS, Ma MC
Basic research in cardiology109(4)414(2014 Jul)

Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages.
Deng B, Wang CW, Arnardottir HH, Li Y, Cheng CY, Dalli J, Serhan CN
PloS one9(7)e102362(2014)

Functional characterization of genetic enzyme variations in human lipoxygenases.
Horn T, Reddy Kakularam K, Anton M, Richter C, Reddanna P, Kuhn H
Redox biology1566-77(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Angiotensin II type 1 receptor blocker potentially ameliorates diabetic nephropathy via the up-regulation of glomerular nephrin and P-cadherin expression through the inhibition of 12-LO activation in the glomeruli of rats with type 2 diabetes.
Title: Effect of angiotensin II type 1 receptor blocker on 12-lipoxygenase activity and slit diaphragm protein expression in type 2 diabetic rat glomeruli.

The aim of the present study was to examine the impacts and mechanisms of 12lipoxygenase (12LO) and its metabolites on the acetylation and methylation of histone3lysine (H3K) in the p21 gene. associated epigenetic changes, including increased histone H3K9Ac and H3K4Me1, and reduced H3K9M.e3 may involved in 12 (S)hydroxyeicosatetraenoic acid mediated p21 gene regulation.
Title: Role of histone modification in 12?lipoxygenase?associated p21 gene regulation.

Hypoxic preconditioning reduces heart susceptibly to ischemia-reperfusion injuryvia ALOX12/TRPV1/PKC pathway.
Title: Hypoxic preconditioning protects rat hearts against ischemia-reperfusion injury via the arachidonate12-lipoxygenase/transient receptor potential vanilloid 1 pathway.

Diabetic proteinuria is mediated by the activation of 12-LO pathway that is partially attributed to the decreased glomerular P-cadherin expression.
Title: Role of 12-lipoxygenase in decreasing P-cadherin and increasing angiotensin II type 1 receptor expression according to glomerular size in type 2 diabetic rats.

Role of Src tyrosine kinase in the atherogenic effects of the 12/15-lipoxygenase pathway in vascular smooth muscle cells.
Title: Role of Src tyrosine kinase in the atherogenic effects of the 12/15-lipoxygenase pathway in vascular smooth muscle cells.

The following Alox12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11436	NM_001105798.1 Latest version!	Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa33277	XM_006246659.3 Latest version!	Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alox12 ( NM_001105798.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099268.1 Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), mRNA.

Price & Availability↑

CloneID	ORa11436
Clone ID Related Accession (Same CDS sequence)	NM_001105798.1
Accession Version	NM_001105798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	arachidonate 12-lipoxygenase, 12S-type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_213369.4, XM_001079612.1, XM_001079600.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCGCT ACCGCGTCCG CGTGGTCACT GGGCCCTGGC TCTTCTCGGG ATCCGTCAAC 
CTGGTGCGTC TGTGGCTGGT CGGGGCGCAC AGGGAGGCGA GGCTGGAGCT GCAGCTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAT TTCGATGTTG CCGAGGACTT GGGGCCACTG 
CAGTTCGTGA AACTGCACAA AGAGCACACA GTTGTGGACG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCCGG GATGAGCGCA GAGGCCGTCT TCCCCTGCTA CCGCTGGGTG 
CAGGGAGAAG GAATCCTGAG ACTGCCGGAG GGGACAGCCC GTCTGGCAGG AGACAATGCC 
TTGGATGTCT TCCAGAAGCA TCGAGAGAAG GAACTGAAGG AAAGACAACA GACCTACCGC 
TGGGCCACCT GGAAGCAAGG CTTACCTCAG ACAATAGCAG CAGACTGTAA GGATGACCTC 
CCTCCAAACA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GCTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCGTCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCTGAGAA GGTTCACCGG 
TGTTGGCAGG AAGATGAGCT CTTTGGCTAC CAGTTTCTCA ATGGCGCCAA CCCCATGCTG 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCCCTGGGAC AGAGGAGCTT 
CAAGCGCAGC TGGAGAAAGA ACTCAAGGAT GGATGCCTGT TTGAGGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACAGC AGTACCTGGC TGCCCCTCTT 
GTCATGTTGA GGATGGACCC CAGTGGGAAG CTGCTCCCCA TGGCTATCCA GATTCAGCCT 
CCCAATCCCA GCTCCCCAGC TCCAACATTG TTCCTGCCCT CAGATCCTCC ACTTGCCTGG 
CTCTTGGCTA AGATCTGGGT CCGAAATTCA GATTTCCAAC TGCAGGAGCT CCAATTCCAT 
TTGCTGAACA CTCATCTGGT GGCTGAGGTC ATTGCTGTTG CCACCATGAG GTGCCTCCCA 
GGACTACACC CTATCTTTAA GCTCCTAGTT CCGCACATCC GTTACACTAT GGAAATTAAC 
ACACGGAGCC GGACCCAGCT CATCTCAGAT GGAGGGATAT TTGATCAGGT AGTGAGCACA 
GGTGGAGGCG GCCATGTTCA GTTGCTCACT CGGGCAGTGG CTCAGCTGAC GTACTGCTCC 
CTCTGTCCTC CCGATGATCT GGCTACCCGA GGCCTGCTGA GAGTCCCAAG TGCCCTCTAC 
GCTCAGGATG CTTTGCAGCT CTGGGAGGTC ACTGCCAGGT ATGTGAACGG GATGGTCCAT 
CTCTTCTACC AGAGTGACGA TATTGTGAGG GGAGACCCAG AGCTGCAGGC CTGGTGTCGG 
GAGATCACTG AGGTGGGGCT GTGCCATGCC CAGGACAGAG GATTCCCTGT CTCCTTCCAG 
TCCCGGGCAC AGCTCTGCCA TTTCCTTACC ATGTGTGTTT TCACATGTAC TTCCCAGCAT 
GCTGCCATCA ACCAGGGCCA GCTGGACTGG TACGGTTGGG TACCTAATGC TCCATGCACA 
ATGAGGATGC CTCCACCAAC CAGCAAGGAC GATGTGACGA TGGAGACCAT CATGGGCTCA 
CTCCCTGATG TTCAGAAGTC CTGTCTGCAG ATGACCATCA CATGGCACCT GGGCCGCCTC 
CAGCCAGACA TGGTACCTCT AGGACACCAC AAAGAAAAGT ATTTCTCAGA CCCAAGGACC 
AAAGCTGTGC TCAGCCAATT TCAAGCAGAT CTGGAAAATT TGGAGAAAGA GATTACAGCC 
CGGAACCAGC AGCTTGACCT GCCCTACGAA TACCTCAAGC CTAGCCGTAT AGAGAACAGC 
ATCACCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099268.1
CDS	48..2039
Translation	MGRYRVRVVTGPWLFSGSVNLVRLWLVGAHREARLELQLRPARGKEEEFDFDVAEDLGPLQFVKLHKEHTVVDDAWFCNLITVQGPGMSAEAVFPCYRWVQGEGILRLPEGTARLAGDNALDVFQKHREKELKERQQTYRWATWKQGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNRLEDFDQIFWGQKSALAEKVHRCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPPGTEELQAQLEKELKDGCLFEADFILLDGIPANVIRGEQQYLAAPLVMLRMDPSGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYCSLCPPDDLATRGLLRVPSALYAQDALQLWEVTARYVNGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTSQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETIMGSLPDVQKSCLQMTITWHLGRLQPDMVPLGHHKEKYFSDPRTKAVLSQFQADLENLEKEITARNQQLDLPYEYLKPSRIENSITI

Target ORF information:

RefSeq Version	NM_001105798.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105798.1

ORF Insert Sequence:

ATGGGCCGCT ACCGCGTCCG CGTGGTCACT GGGCCCTGGC TCTTCTCGGG ATCCGTCAAC 
CTGGTGCGTC TGTGGCTGGT CGGGGCGCAC AGGGAGGCGA GGCTGGAGCT GCAGCTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAT TTCGATGTTG CCGAGGACTT GGGGCCACTG 
CAGTTCGTGA AACTGCACAA AGAGCACACA GTTGTGGACG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCCGG GATGAGCGCA GAGGCCGTCT TCCCCTGCTA CCGCTGGGTG 
CAGGGAGAAG GAATCCTGAG ACTGCCGGAG GGGACAGCCC GTCTGGCAGG AGACAATGCC 
TTGGATGTCT TCCAGAAGCA TCGAGAGAAG GAACTGAAGG AAAGACAACA GACCTACCGC 
TGGGCCACCT GGAAGCAAGG CTTACCTCAG ACAATAGCAG CAGACTGTAA GGATGACCTC 
CCTCCAAACA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GCTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCGTCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCTGAGAA GGTTCACCGG 
TGTTGGCAGG AAGATGAGCT CTTTGGCTAC CAGTTTCTCA ATGGCGCCAA CCCCATGCTG 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCCCTGGGAC AGAGGAGCTT 
CAAGCGCAGC TGGAGAAAGA ACTCAAGGAT GGATGCCTGT TTGAGGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACAGC AGTACCTGGC TGCCCCTCTT 
GTCATGTTGA GGATGGACCC CAGTGGGAAG CTGCTCCCCA TGGCTATCCA GATTCAGCCT 
CCCAATCCCA GCTCCCCAGC TCCAACATTG TTCCTGCCCT CAGATCCTCC ACTTGCCTGG 
CTCTTGGCTA AGATCTGGGT CCGAAATTCA GATTTCCAAC TGCAGGAGCT CCAATTCCAT 
TTGCTGAACA CTCATCTGGT GGCTGAGGTC ATTGCTGTTG CCACCATGAG GTGCCTCCCA 
GGACTACACC CTATCTTTAA GCTCCTAGTT CCGCACATCC GTTACACTAT GGAAATTAAC 
ACACGGAGCC GGACCCAGCT CATCTCAGAT GGAGGGATAT TTGATCAGGT AGTGAGCACA 
GGTGGAGGCG GCCATGTTCA GTTGCTCACT CGGGCAGTGG CTCAGCTGAC GTACTGCTCC 
CTCTGTCCTC CCGATGATCT GGCTACCCGA GGCCTGCTGA GAGTCCCAAG TGCCCTCTAC 
GCTCAGGATG CTTTGCAGCT CTGGGAGGTC ACTGCCAGGT ATGTGAACGG GATGGTCCAT 
CTCTTCTACC AGAGTGACGA TATTGTGAGG GGAGACCCAG AGCTGCAGGC CTGGTGTCGG 
GAGATCACTG AGGTGGGGCT GTGCCATGCC CAGGACAGAG GATTCCCTGT CTCCTTCCAG 
TCCCGGGCAC AGCTCTGCCA TTTCCTTACC ATGTGTGTTT TCACATGTAC TTCCCAGCAT 
GCTGCCATCA ACCAGGGCCA GCTGGACTGG TACGGTTGGG TACCTAATGC TCCATGCACA 
ATGAGGATGC CTCCACCAAC CAGCAAGGAC GATGTGACGA TGGAGACCAT CATGGGCTCA 
CTCCCTGATG TTCAGAAGTC CTGTCTGCAG ATGACCATCA CATGGCACCT GGGCCGCCTC 
CAGCCAGACA TGGTACCTCT AGGACACCAC AAAGAAAAGT ATTTCTCAGA CCCAAGGACC 
AAAGCTGTGC TCAGCCAATT TCAAGCAGAT CTGGAAAATT TGGAGAAAGA GATTACAGCC 
CGGAACCAGC AGCTTGACCT GCCCTACGAA TACCTCAAGC CTAGCCGTAT AGAGAACAGC 
ATCACCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12 ( XM_006246659.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246721.1 Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33277
Clone ID Related Accession (Same CDS sequence)	XM_006246659.3
Accession Version	XM_006246659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arachidonate 12-lipoxygenase, 12S-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGCT ACCGCGTCCG CGTGGTCACT GGGGCCTGGC TCTTCTCGGG ATCCGTCAAC 
CTGGTGCGTC TGTGGCTGGT CGGGGCGCAC AGGGAGGCGA GGCTGGAGCT GCAGCTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAT TTCGATGTTG CCGAGGACTT GGGGCCACTG 
CAGTTCGTGA AACTGCACAA AGAGCACACA GTTGTGGACG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCCGG GATGAGCGCA GAGGCCGTCT TCCCCTGCTA CCGCTGGGTG 
CAGGGAGAAG GAATCCTGAG ACTGCCGGAG GGGACAGCCC GTCTGGCAGG AGACAATGCC 
TTGGATGTCT TCCAGAAGCA TCGAGAGAAG GAACTGAAGG AAAGACAACA GACCTACCGC 
TGGGCCACCT GGAAGCAAGG CTTACCTCAG ACAATAGCAG CAGACTGTAA GGATGACCTC 
CCTCCAAACA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GCTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCGTCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCTGAGAA GGTTCACCGG 
TGTTGGCAGG AAGATGAGCT CTTTGGCTAC CAGTTTCTCA ATGGCGCCAA CCCCATGCTG 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCCCTGGGAC AGAGGAGCTT 
CAAGCGCAGC TGGAGAAAGA ACTCAAGGAT GGATGCCTGT TTGAGGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACAGC AGTACCTGGC TGCCCCTCTT 
GTCATGTTGA GGATGGACCC CAGTGGGAAG CTGCTCCCCA TGGCTATCCA GCTCCTAGTT 
CCGCACATCC GTTACACTAT GGAAATTAAC ACACGGAGCC GGACCCAGCT CATCTCAGAT 
GGAGGGATAT TTGATCAGGT AGTGAGCACA GGTGGAGGCG GCCATGTTCA GTTGCTCACT 
CGGGCAGTGG CTCAGCTGAC GTACTGCTCC CTCTGTCCTC CCGATGATCT GGCTACCCGA 
GGCCTGCTGA GAGTCCCAAG TGCCCTCTAC GCTCAGGATG CTTTGCAGCT CTGGGAGGTC 
ACTGCCAGGT ATGTGAACGG GATGGTCCAT CTCTTCTACC AGAGTGACGA TATTGTGAGG 
GGAGACCCAG AGCTGCAGGC CTGGTGTCGG GAGATCACTG AGGTGGGGCT GTGCCATGCC 
CAGGACAGAG GATTCCCTGT CTCCTTCCAG TCCCGGGCAC AGCTCTGCCA TTTCCTTACC 
ATGTGTGTTT TCACATGTAC TTCCCAGCAT GCTGCCATCA ACCAGGGCCA GCTGGACTGG 
TACGGTTGGG TACCTAATGC TCCATGCACA ATGAGGATGC CTCCACCAAC CAGCAAGGAC 
GATGTGACGA TGGAGACCAT CATGGGCTCA CTCCCTGATG TTCAGAAGTC CTGTCTGCAG 
ATGACCATCA CATGGCACCT GGGCCGCCTC CAGCCAGACA TGGTACCTCT AGGACACCAC 
AAAGAAAAGT ATTTCTCAGA CCCAAGGACC AAAGCTGTGC TCAGCCAATT TCAAGCAGAT 
CTGGAAAATT TGGAGAAAGA GATTACAGCC CGGAACCAGC AGCTTGACCT GCCCTACGAA 
TACCTCAAGC CTAGCCGTAT AGAGAACAGC ATCACCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246721.1
CDS	92..1873
Translation	MGRYRVRVVTGAWLFSGSVNLVRLWLVGAHREARLELQLRPARGKEEEFDFDVAEDLGPLQFVKLHKEHTVVDDAWFCNLITVQGPGMSAEAVFPCYRWVQGEGILRLPEGTARLAGDNALDVFQKHREKELKERQQTYRWATWKQGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNRLEDFDQIFWGQKSALAEKVHRCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPPGTEELQAQLEKELKDGCLFEADFILLDGIPANVIRGEQQYLAAPLVMLRMDPSGKLLPMAIQLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYCSLCPPDDLATRGLLRVPSALYAQDALQLWEVTARYVNGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTSQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETIMGSLPDVQKSCLQMTITWHLGRLQPDMVPLGHHKEKYFSDPRTKAVLSQFQADLENLEKEITARNQQLDLPYEYLKPSRIENSITI

Target ORF information:

RefSeq Version	XM_006246659.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus arachidonate 12-lipoxygenase, 12S type (Alox12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246659.3

ORF Insert Sequence:

ATGGGCCGCT ACCGCGTCCG CGTGGTCACT GGGGCCTGGC TCTTCTCGGG ATCCGTCAAC 
CTGGTGCGTC TGTGGCTGGT CGGGGCGCAC AGGGAGGCGA GGCTGGAGCT GCAGCTGCGG 
CCAGCGCGGG GCAAGGAGGA GGAGTTTGAT TTCGATGTTG CCGAGGACTT GGGGCCACTG 
CAGTTCGTGA AACTGCACAA AGAGCACACA GTTGTGGACG ACGCCTGGTT CTGCAACCTC 
ATCACAGTTC AGGGGCCCGG GATGAGCGCA GAGGCCGTCT TCCCCTGCTA CCGCTGGGTG 
CAGGGAGAAG GAATCCTGAG ACTGCCGGAG GGGACAGCCC GTCTGGCAGG AGACAATGCC 
TTGGATGTCT TCCAGAAGCA TCGAGAGAAG GAACTGAAGG AAAGACAACA GACCTACCGC 
TGGGCCACCT GGAAGCAAGG CTTACCTCAG ACAATAGCAG CAGACTGTAA GGATGACCTC 
CCTCCAAACA TGAGATTCCA TGAGGAGAAG AGACTGGACT TTGAATGGAC GCTGAAGGCG 
GGGGTTCTGG AAATGGGCCT CAAACGTGTT TATACCCTCC TGAGAAGCTG GAACCGTCTG 
GAAGACTTTG ATCAGATCTT CTGGGGCCAG AAGAGTGCCT TGGCTGAGAA GGTTCACCGG 
TGTTGGCAGG AAGATGAGCT CTTTGGCTAC CAGTTTCTCA ATGGCGCCAA CCCCATGCTG 
TTGAGACGCT CCACCTCTCT GCCCTCCAGA CTGGTACTGC CCCCTGGGAC AGAGGAGCTT 
CAAGCGCAGC TGGAGAAAGA ACTCAAGGAT GGATGCCTGT TTGAGGCTGA CTTTATCCTG 
CTGGATGGAA TTCCAGCTAA TGTGATCCGA GGAGAACAGC AGTACCTGGC TGCCCCTCTT 
GTCATGTTGA GGATGGACCC CAGTGGGAAG CTGCTCCCCA TGGCTATCCA GCTCCTAGTT 
CCGCACATCC GTTACACTAT GGAAATTAAC ACACGGAGCC GGACCCAGCT CATCTCAGAT 
GGAGGGATAT TTGATCAGGT AGTGAGCACA GGTGGAGGCG GCCATGTTCA GTTGCTCACT 
CGGGCAGTGG CTCAGCTGAC GTACTGCTCC CTCTGTCCTC CCGATGATCT GGCTACCCGA 
GGCCTGCTGA GAGTCCCAAG TGCCCTCTAC GCTCAGGATG CTTTGCAGCT CTGGGAGGTC 
ACTGCCAGGT ATGTGAACGG GATGGTCCAT CTCTTCTACC AGAGTGACGA TATTGTGAGG 
GGAGACCCAG AGCTGCAGGC CTGGTGTCGG GAGATCACTG AGGTGGGGCT GTGCCATGCC 
CAGGACAGAG GATTCCCTGT CTCCTTCCAG TCCCGGGCAC AGCTCTGCCA TTTCCTTACC 
ATGTGTGTTT TCACATGTAC TTCCCAGCAT GCTGCCATCA ACCAGGGCCA GCTGGACTGG 
TACGGTTGGG TACCTAATGC TCCATGCACA ATGAGGATGC CTCCACCAAC CAGCAAGGAC 
GATGTGACGA TGGAGACCAT CATGGGCTCA CTCCCTGATG TTCAGAAGTC CTGTCTGCAG 
ATGACCATCA CATGGCACCT GGGCCGCCTC CAGCCAGACA TGGTACCTCT AGGACACCAC 
AAAGAAAAGT ATTTCTCAGA CCCAAGGACC AAAGCTGTGC TCAGCCAATT TCAAGCAGAT 
CTGGAAAATT TGGAGAAAGA GATTACAGCC CGGAACCAGC AGCTTGACCT GCCCTACGAA 
TACCTCAAGC CTAGCCGTAT AGAGAACAGC ATCACCATCT GA