Polr2j cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Polr2j
Entrez Gene ID	288588
Full Name	RNA polymerase II subunit J
General protein information	Preferred Names RNA polymerase II subunit J Names DNA-directed RNA polymerase II subunit RPB11-a polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa polymerase (RNA) II subunit J
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12

mRNA and Protein(s)

mRNA Protein Name

NM_001105921.1 NP_001099391.1 DNA-directed RNA polymerase II subunit RPB11-a
Pathways from BioSystems

mRNA	Protein	Name
NM_001105921.1	NP_001099391.1	DNA-directed RNA polymerase II subunit RPB11-a

Homologies

Canis lupus familiaris (dog)	LOC100686835	XP_003434782.2
Xenopus tropicalis (tropical clawed frog)	polr2j	NP_001039095.1
Pan troglodytes (chimpanzee)	POLR2J	XP_003949275.1
Drosophila melanogaster (fruit fly)	Rpb11	NP_609836.1
Bos taurus (cattle)	POLR2J	NP_001032560.1
Rattus norvegicus (Norway rat)	Polr2j	NP_001099391.1
Danio rerio (zebrafish)	polr2j	NP_001019571.1
Arabidopsis thaliana (thale cress)	NRPB11	NP_190777.1
Oryza sativa (rice)	Os07g0172500	NP_001058998.1
Caenorhabditis elegans (roundworm)	rpb-11	NP_496942.1
Homo sapiens (human)	POLR2J	NP_006225.1
Mus musculus (house mouse)	Polr2j	NP_035423.2
Anopheles gambiae (African malaria mosquito)	RPB11_ANOGA	XP_319985.2

Gene Ontology
Bibliography

Related articles in PubMed

TFIIB-related factors in RNA polymerase I transcription.
Knutson BA, Hahn S
Biochimica et biophysica acta1829(3-4)265-73(2013 Mar-Apr)

RNA polymerase II subunit 3 is retained in the cytoplasm by its interaction with HCR, the psoriasis vulgaris candidate gene product.
Corbi N, Bruno T, De Angelis R, Di Padova M, Libri V, Di Certo MG, Spinardi L, Floridi A, Fanciulli M, Passananti C
Journal of cell science118(Pt 18)4253-60(2005 Sep)

Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb.
Fanciulli M, Bruno T, Di Padova M, De Angelis R, Iezzi S, Iacobini C, Floridi A, Passananti C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology14(7)904-12(2000 May)

Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes.
Kershnar E, Wu SY, Chiang CM
The Journal of biological chemistry273(51)34444-53(1998 Dec)

Isolation and characterization of cDNA encoding mouse RNA polymerase II subunit RPB14.
Nishi Y, Yamamoto K, Yao Y, Yamamoto M, Nogi Y, Matsuo H, Muramatsu M
Gene187(2)165-70(1997 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr2j gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2j cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11678	NM_001105921.1 Latest version!	Rattus norvegicus RNA polymerase II subunit J (Polr2j), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2j ( NM_001105921.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099391.1 Rattus norvegicus RNA polymerase II subunit J (Polr2j), mRNA.

Price & Availability↑

CloneID	ORa11678
Clone ID Related Accession (Same CDS sequence)	NM_001105921.1
Accession Version	NM_001105921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase II subunit RPB11-a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473973.1. On or before Oct 3, 2007 this sequence version replaced XM_213753.3, XM_001077131.1. ##Evidence-Data-START## Transcript exon combination :: AW919016.1, FM080184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGAACGCCC CTCCGGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GAAAATCACC 
ATTAACAAGG ACACCAAGGT GCCCAACGCT TGCTTGTTCA CCATCAACAA AGAAGACCAC 
ACTCTGGGGA ACATCATTAA ATCGCAGCTG TTGAAGGACC CGCAGGTGCT GTTTGCCGGC 
TACAAAGTCC CTCACCCCTT GGAGCACAAG ATCATCATTC GTGTGCAGAC CACCCCAGAC 
TACAGCCCCC AGGAGGCTTT CACCAATGCC ATCACAGACC TCATCAGTGA GCTCTCCCTC 
CTGGAGGAGC GATTCCGGGT GGCCATCAAG GACAAGCAAG AAGGAATTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099391.1
CDS	53..406
Translation	MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE

Target ORF information:

RefSeq Version	NM_001105921.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA polymerase II subunit J (Polr2j), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105921.1

ORF Insert Sequence:

ATGAACGCCC CTCCGGCCTT CGAGTCGTTC TTGCTCTTCG AGGGCGAGAA GAAAATCACC 
ATTAACAAGG ACACCAAGGT GCCCAACGCT TGCTTGTTCA CCATCAACAA AGAAGACCAC 
ACTCTGGGGA ACATCATTAA ATCGCAGCTG TTGAAGGACC CGCAGGTGCT GTTTGCCGGC 
TACAAAGTCC CTCACCCCTT GGAGCACAAG ATCATCATTC GTGTGCAGAC CACCCCAGAC 
TACAGCCCCC AGGAGGCTTT CACCAATGCC ATCACAGACC TCATCAGTGA GCTCTCCCTC 
CTGGAGGAGC GATTCCGGGT GGCCATCAAG GACAAGCAAG AAGGAATTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TFIIB-related factors in RNA polymerase I transcription.
Biochimica et biophysica acta1829(3-4)265-73(2013 Mar-Apr)
Knutson BA,Hahn S

RNA polymerase II subunit 3 is retained in the cytoplasm by its interaction with HCR, the psoriasis vulgaris candidate gene product.
Journal of cell science118(Pt 18)4253-60(2005 Sep)
Corbi N,Bruno T,De Angelis R,Di Padova M,Libri V,Di Certo MG,Spinardi L,Floridi A,Fanciulli M,Passananti C

Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology14(7)904-12(2000 May)
Fanciulli M,Bruno T,Di Padova M,De Angelis R,Iezzi S,Iacobini C,Floridi A,Passananti C

Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes.
The Journal of biological chemistry273(51)34444-53(1998 Dec)
Kershnar E,Wu SY,Chiang CM

Isolation and characterization of cDNA encoding mouse RNA polymerase II subunit RPB14.
Gene187(2)165-70(1997 Mar)
Nishi Y,Yamamoto K,Yao Y,Yamamoto M,Nogi Y,Matsuo H,Muramatsu M

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Polr2j cDNA ORF clone, Rattus norvegicus(Norway rat)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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