Nedd9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Nedd9
Entrez Gene ID	291044
Full Name	neural precursor cell expressed, developmentally down-regulated 9
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 9 Names enhancer of filamentation 1 neural precursor cell expressed, developmentally down-regulated gene 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p13

mRNA and Protein(s)

mRNA Protein Name

XM_006253786.3 XP_006253848.1 enhancer of filamentation 1 isoform X1

NM_001011922.1 NP_001011922.1 enhancer of filamentation 1
Pathways from BioSystems

mRNA	Protein	Name
XM_006253786.3	XP_006253848.1	enhancer of filamentation 1 isoform X1
NM_001011922.1	NP_001011922.1	enhancer of filamentation 1

Homologies

Danio rerio (zebrafish)	nedd9	NP_001070750.1
Mus musculus (house mouse)	Nedd9	NP_059492.3
Gallus gallus (chicken)	NEDD9	XP_418946.4
Xenopus tropicalis (tropical clawed frog)	nedd9	XP_002932712.1
Canis lupus familiaris (dog)	NEDD9	XP_545342.4
Bos taurus (cattle)	NEDD9	NP_001095317.2
Homo sapiens (human)	NEDD9	NP_006394.1
	NEDD9	XP_001089291.1
Rattus norvegicus (Norway rat)	Nedd9	NP_001011922.1
Danio rerio (zebrafish)	LOC100536178	XP_003200560.1

Gene Ontology
Bibliography

Related articles in PubMed

Retinoic acid regulates Schwann cell migration via NEDD9 induction by transcriptional and post-translational mechanisms.
Latasa MJ, Jim?nez-Lara AM, Cosgaya JM
Biochimica et biophysica acta1863(7 Pt A)1510-8(2016 Jul)

CRK proteins selectively regulate T cell migration into inflamed tissues.
Huang Y, Clarke F, Karimi M, Roy NH, Williamson EK, Okumura M, Mochizuki K, Chen EJ, Park TJ, Debes GF, Zhang Y, Curran T, Kambayashi T, Burkhardt JK
The Journal of clinical investigation125(3)1019-32(2015 Mar)

Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis.
Zheng P, Dean J
Proceedings of the National Academy of Sciences of the United States of America106(18)7473-8(2009 May)

A novel Cas family member, HEPL, regulates FAK and cell spreading.
Singh MK, Dadke D, Nicolas E, Serebriiskii IG, Apostolou S, Canutescu A, Egleston BL, Golemis EA
Molecular biology of the cell19(4)1627-36(2008 Apr)

Nedd9 protein, a Cas-L homologue, is upregulated after transient global ischemia in rats: possible involvement of Nedd9 in the differentiation of neurons after ischemia.
Sasaki T, Iwata S, Okano HJ, Urasaki Y, Hamada J, Tanaka H, Dang NH, Okano H, Morimoto C
Stroke36(11)2457-62(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Retinoic acid regulates Schwann cell migration by inducing NEDD9 levels by both transcriptional and post-translational mechanisms.
Title: Retinoic acid regulates Schwann cell migration via NEDD9 induction by transcriptional and post-translational mechanisms.

Nedd9 has a role in the differentiation of neurons after ischemia
Title: Nedd9 protein, a Cas-L homologue, is upregulated after transient global ischemia in rats: possible involvement of Nedd9 in the differentiation of neurons after ischemia.

Nedd9_predicted is regulated in the embryonic hindbrain by all-trans retinoic acid.
Title: Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid.

The following Nedd9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01536	NM_001011922.1 Latest version!	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa37800	XM_006253786.3 Latest version!	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd9 ( NM_001011922.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001011922.1 Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), mRNA.

Price & Availability↑

CloneID	ORa01536
Clone ID Related Accession (Same CDS sequence)	NM_001011922.1
Accession Version	NM_001011922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	enhancer of filamentation 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085812.1. On Feb 11, 2005 this sequence version replaced XM_225245.2. ##Evidence-Data-START## Transcript exon combination :: BC085812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTATA AGAATCTTAT GGCGAGGGCC TTGTATGACA ATGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTG ACCGTCATAG AGCAGAACAC AGGAGGACTA 
GAGGGATGGT GGCTGTGTTC CCTACATGGT CGCCAAGGCA TCGTCCCAGG TAACCGGGTG 
AAACTTCTGA TTGGTCCAGT GCAAGAGCCC TCCAGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA ACTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCACCT 
CGTGATACCA TCTACCAAGT GCCGCCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCAGCA CTAATGGACC CCATCTAGGC AAAAAGGTGA TCACGCCAGT GAGGGTTGGC 
CATGGCTATG TGTACGAGCA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACACCA CTCAAGGGGT ATATGACATC CCTCCTTCAT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGTT AAAACCTCAA GGGGTATACG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC AGCTTGCCGG GATGAGGCGG GGCTGAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA ACAAGACGGA AAACTGGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGTG GGGAAGGACC TTCACACCAA ATTCCCCTGT 
GATGCTCCAG GAGGGGCAGA ACCAATGGCA CGAAGACACC AGAGCCTTTC CCTGCACCAC 
GCACCCTCTC AGGTGGGACC GTCCGGGGAC ACTCAGAGTG ATGCATATGA TGTCCCCCGG 
GGAGTTCAGT TTCTCGAGGT ACCGACAGAA TCCAGTGAAA AGGCAAACCT GGAGGAGAGA 
GACGGTGTTT ATGATGTCCC TCTGCACAAC CCAGCGGATG CTAAAGGCTC TCGGGACGTG 
GTAGACGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACATCTTCCA CCTCCTCAAA GGAGTCCTCG TTGTCAGCTT CTCCGTCTCA GGACAAAAGG 
CTCCTCCTGG ACCCAGACAC AGCCATTGGG AAGCTCTATT GGCTCCAGCA GACCCTGGAG 
ACGGGCATCT GCAGCCTCAT GTCACTGGTC ACCATAGACT GGAGGTCTTA TGGATACATG 
GAAAGGCACA TCAACGAGAT CCGCACCGCA GTGGACAAAG TGGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAGGGG CGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACCGGTCCTC 
CTCAATAAAA TGAATCGGGA ACTCCAGAGA GTAGACGATT CCCACCAGAT CCTAAGCCAA 
ACCAGCCATG ATCTGAATGA ATGCAGCTGG TCCCTGAATG TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTGG CCAAGACAGT GCCCGACGAT 
GCCAAGCAAC TGACCACCAC CATCAGCACC TACGCAGAGA CTCTCTTTAG AGTAGATCCT 
GGCAATTCCC ATCTGAAGAA TGGGCCTGAC AGTATCACGA ACTCAATCGA GTACACACAT 
ACGGGCTCCC AGATGCAGCC ACTGCGCCCC GGTGACTACA AAGGCCAGGT TCACAATAAG 
CCATTGCCTC CTACTCTAAG CAAGGAGCAG CCACCAGACT GCAGCAGCAG TGACGGTTCC 
GAGAGGAGTT GGATGGACGA TTATGACTAT GTCCACCTGC AGGGCAAAGA GGAGTTTGAG 
AGACAACAGA AGGAACTCTT GGAAAAAGAG AACATCATGA AGCAGAGCAA GGCGCAGCTG 
GAGCATCATC AGCTGAGTCA GTTCCAGCTG TTAGAACAAG AAATCACCAA GCCCGTGGAG 
AATGACATTT CCAAATGGAA ACCCTCTCAG AGCCTCCCAA CCACAAACAA CAGTGTGGGT 
GCTCAGGATA GGCAATTGCT TTGCTTCTAC TATGACCAAT GCGAGACCCA TTTCATCTCC 
CTCCTCAATG CCATTGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAGCC CCCGCGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTC AGTGCACACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC CGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATTGTG ATGGCGACCA AGATGGCCGC CCTCCACTAC 
CCGAGCACTA CGGCTTTACA GGAAATGGTG CACCAAGTAA CAGACCTGTC CAGAAATGCT 
CAACTGTTCA AGCGTTCCTT GCTGGAGATG GCCACCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011922.1
CDS	104..2605
Translation	MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQEPSSHEQPTPGPMHQNFGQQKLYQVPNSQAAPRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGSTNGPHLGKKVITPVRVGHGYVYEHPSRYQKDVYDVPPSHTTQGVYDIPPSSVKGPVFSVPVGELKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKLDTRPEGVYDIPPTSTKTVGKDLHTKFPCDAPGGAEPMARRHQSLSLHHAPSQVGPSGDTQSDAYDVPRGVQFLEVPTESSEKANLEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLLLDPDTAIGKLYWLQQTLETGICSLMSLVTIDWRSYGYMERHINEIRTAVDKVELFLREYLHFARGALANASCLPEPVLLNKMNRELQRVDDSHQILSQTSHDLNECSWSLNVLAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRVDPGNSHLKNGPDSITNSIEYTHTGSQMQPLRPGDYKGQVHNKPLPPTLSKEQPPDCSSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	NM_001011922.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011922.1

ORF Insert Sequence:

ATGAAGTATA AGAATCTTAT GGCGAGGGCC TTGTATGACA ATGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTG ACCGTCATAG AGCAGAACAC AGGAGGACTA 
GAGGGATGGT GGCTGTGTTC CCTACATGGT CGCCAAGGCA TCGTCCCAGG TAACCGGGTG 
AAACTTCTGA TTGGTCCAGT GCAAGAGCCC TCCAGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA ACTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCACCT 
CGTGATACCA TCTACCAAGT GCCGCCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCAGCA CTAATGGACC CCATCTAGGC AAAAAGGTGA TCACGCCAGT GAGGGTTGGC 
CATGGCTATG TGTACGAGCA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACACCA CTCAAGGGGT ATATGACATC CCTCCTTCAT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGTT AAAACCTCAA GGGGTATACG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC AGCTTGCCGG GATGAGGCGG GGCTGAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA ACAAGACGGA AAACTGGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGTG GGGAAGGACC TTCACACCAA ATTCCCCTGT 
GATGCTCCAG GAGGGGCAGA ACCAATGGCA CGAAGACACC AGAGCCTTTC CCTGCACCAC 
GCACCCTCTC AGGTGGGACC GTCCGGGGAC ACTCAGAGTG ATGCATATGA TGTCCCCCGG 
GGAGTTCAGT TTCTCGAGGT ACCGACAGAA TCCAGTGAAA AGGCAAACCT GGAGGAGAGA 
GACGGTGTTT ATGATGTCCC TCTGCACAAC CCAGCGGATG CTAAAGGCTC TCGGGACGTG 
GTAGACGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACATCTTCCA CCTCCTCAAA GGAGTCCTCG TTGTCAGCTT CTCCGTCTCA GGACAAAAGG 
CTCCTCCTGG ACCCAGACAC AGCCATTGGG AAGCTCTATT GGCTCCAGCA GACCCTGGAG 
ACGGGCATCT GCAGCCTCAT GTCACTGGTC ACCATAGACT GGAGGTCTTA TGGATACATG 
GAAAGGCACA TCAACGAGAT CCGCACCGCA GTGGACAAAG TGGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAGGGG CGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACCGGTCCTC 
CTCAATAAAA TGAATCGGGA ACTCCAGAGA GTAGACGATT CCCACCAGAT CCTAAGCCAA 
ACCAGCCATG ATCTGAATGA ATGCAGCTGG TCCCTGAATG TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTGG CCAAGACAGT GCCCGACGAT 
GCCAAGCAAC TGACCACCAC CATCAGCACC TACGCAGAGA CTCTCTTTAG AGTAGATCCT 
GGCAATTCCC ATCTGAAGAA TGGGCCTGAC AGTATCACGA ACTCAATCGA GTACACACAT 
ACGGGCTCCC AGATGCAGCC ACTGCGCCCC GGTGACTACA AAGGCCAGGT TCACAATAAG 
CCATTGCCTC CTACTCTAAG CAAGGAGCAG CCACCAGACT GCAGCAGCAG TGACGGTTCC 
GAGAGGAGTT GGATGGACGA TTATGACTAT GTCCACCTGC AGGGCAAAGA GGAGTTTGAG 
AGACAACAGA AGGAACTCTT GGAAAAAGAG AACATCATGA AGCAGAGCAA GGCGCAGCTG 
GAGCATCATC AGCTGAGTCA GTTCCAGCTG TTAGAACAAG AAATCACCAA GCCCGTGGAG 
AATGACATTT CCAAATGGAA ACCCTCTCAG AGCCTCCCAA CCACAAACAA CAGTGTGGGT 
GCTCAGGATA GGCAATTGCT TTGCTTCTAC TATGACCAAT GCGAGACCCA TTTCATCTCC 
CTCCTCAATG CCATTGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAGCC CCCGCGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTC AGTGCACACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC CGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATTGTG ATGGCGACCA AGATGGCCGC CCTCCACTAC 
CCGAGCACTA CGGCTTTACA GGAAATGGTG CACCAAGTAA CAGACCTGTC CAGAAATGCT 
CAACTGTTCA AGCGTTCCTT GCTGGAGATG GCCACCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd9 ( XM_006253786.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253848.1 Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37800
Clone ID Related Accession (Same CDS sequence)	XM_006253786.3
Accession Version	XM_006253786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	enhancer of filamentation 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA GGAATCTTAT GGCGAGGGCC TTGTATGACA ATGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTG ACCGTCATAG AGCAGAACAC AGGAGGACTA 
GAGGGATGGT GGCTGTGTTC CCTACATGGT CGCCAAGGCA TCGTCCCAGG TAACCGGGTG 
AAACTTCTGA TTGGTCCAGT GCAAGAGCCC TCCAGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA ACTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCACCT 
CGTGATACCA TCTACCAAGT GCCGCCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCAGCA CTAATGGACC CCATCTAGGC AAAAAGGTGA TCACGCCAGT GAGGGTTGGC 
CATGGCTATG TGTACGAGCA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACACCA CTCAAGGGGT ATATGACATC CCTCCTTCAT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGTT AAAACCTCAA GGGGTATACG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC AGCTTGCCGG GATGAGGCGG GGCTGAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA ACAAGACGGA AAACTGGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGTG GGGAAGGACC TTCACACCAA ATTCCCCTGT 
GATGCTCCAG GAGGGGCAGA ACCAATGGCA CGAAGACACC AGAGCCTTTC CCTGCACCAC 
GCACCCTCTC AGGTGGGACC GTCCGGGGAC ACTCAGAGTG ATGCATATGA TGTCCCCCGG 
GGAGTTCAGT TTCTCGAGGT ACCGACAGAA TCCAGTGAAA AGGCAAACCT GGAGGAGAGA 
GACGGTGTTT ATGATGTCCC TCTGCACAAC CCAGCGGATG CTAAAGGCTC TCGGGACGTG 
GTAGACGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACATCTTCCA CCTCCTCAAA GGAGTCCTCG TTGTCAGCTT CTCCGTCTCA GGACAAAAGG 
CTCCTCCTGG ACCCAGACAC AGCCATTGGG AAGCTCTATT GGCTCCAGCA GACCCTGGAG 
ACGGGCATCT GCAGCCTCAT GTCACTGGTC ACCATAGACT GGAGGTCTTA TGGATACATG 
GAAAGGCACA TCAACGAGAT CCGCACCGCA GTGGACAAAG TGGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAGGGG CGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACCGGTCCTC 
CTCAATAAAA TGAATCGGGA ACTCCAGAGA GTAGACGATT CCCACCAGAT CCTAAGCCAA 
ACCAGCCATG ATCTGAATGA ATGCAGCTGG TCCCTGAATG TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTGG CCAAGACAGT GCCCGACGAT 
GCCAAGCAAC TGACCACCAC CATCAGCACC TACGCAGAGA CTCTCTTTAG AGTAGATCCT 
GGCAATTCCC ATCTGAAGAA TGGGCCTGAC AGTATCACGA ACTCAATCGA GTACACACAT 
ACGGGCTCCC AGATGCAGCC ACTGCGCCCC GGTGACTACA AAGGCCAGGT TCACAATAAG 
CCATTGCCTC CTACTCTAAG CAAGGAGCAG CCACCAGACT GCAGCAGCAG TGACGGTTCC 
GAGAGGAGTT GGATGGACGA TTATGACTAT GTCCACCTGC AGGGCAAAGA GGAGTTTGAG 
AGACAACAGA AGGAACTCTT GGAAAAAGAG AACATCATGA AGCAGAGCAA GGCGCAGCTG 
GAGCATCATC AGCTGAGTCA GTTCCAGCTG TTAGAACAAG AAATCACCAA GCCCGTGGAG 
AATGACATTT CCAAATGGAA ACCCTCTCAG AGCCTCCCAA CCACAAACAA CAGTGTGGGT 
GCTCAGGATA GGCAATTGCT TTGCTTCTAC TATGACCAAT GCGAGACCCA TTTCATCTCC 
CTCCTCAATG CCATTGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAGCC CCCGCGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTC AGTGCACACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC CGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATTGTG ATGGCGACCA AGATGGCCGC CCTCCACTAC 
CCGAGCACTA CGGCTTTACA GGAAATGGTG CACCAAGTAA CAGACCTGTC CAGAAATGCT 
CAACTGTTCA AGCGTTCCTT GCTGGAGATG GCCACCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253848.1
CDS	354..2855
Translation	MWARNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQEPSSHEQPTPGPMHQNFGQQKLYQVPNSQAAPRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGSTNGPHLGKKVITPVRVGHGYVYEHPSRYQKDVYDVPPSHTTQGVYDIPPSSVKGPVFSVPVGELKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKLDTRPEGVYDIPPTSTKTVGKDLHTKFPCDAPGGAEPMARRHQSLSLHHAPSQVGPSGDTQSDAYDVPRGVQFLEVPTESSEKANLEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLLLDPDTAIGKLYWLQQTLETGICSLMSLVTIDWRSYGYMERHINEIRTAVDKVELFLREYLHFARGALANASCLPEPVLLNKMNRELQRVDDSHQILSQTSHDLNECSWSLNVLAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRVDPGNSHLKNGPDSITNSIEYTHTGSQMQPLRPGDYKGQVHNKPLPPTLSKEQPPDCSSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	XM_006253786.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 9 (Nedd9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253786.3

ORF Insert Sequence:

ATGTGGGCGA GGAATCTTAT GGCGAGGGCC TTGTATGACA ATGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTG ACCGTCATAG AGCAGAACAC AGGAGGACTA 
GAGGGATGGT GGCTGTGTTC CCTACATGGT CGCCAAGGCA TCGTCCCAGG TAACCGGGTG 
AAACTTCTGA TTGGTCCAGT GCAAGAGCCC TCCAGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA ACTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCACCT 
CGTGATACCA TCTACCAAGT GCCGCCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCAGCA CTAATGGACC CCATCTAGGC AAAAAGGTGA TCACGCCAGT GAGGGTTGGC 
CATGGCTATG TGTACGAGCA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACACCA CTCAAGGGGT ATATGACATC CCTCCTTCAT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGTT AAAACCTCAA GGGGTATACG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC AGCTTGCCGG GATGAGGCGG GGCTGAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA ACAAGACGGA AAACTGGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGTG GGGAAGGACC TTCACACCAA ATTCCCCTGT 
GATGCTCCAG GAGGGGCAGA ACCAATGGCA CGAAGACACC AGAGCCTTTC CCTGCACCAC 
GCACCCTCTC AGGTGGGACC GTCCGGGGAC ACTCAGAGTG ATGCATATGA TGTCCCCCGG 
GGAGTTCAGT TTCTCGAGGT ACCGACAGAA TCCAGTGAAA AGGCAAACCT GGAGGAGAGA 
GACGGTGTTT ATGATGTCCC TCTGCACAAC CCAGCGGATG CTAAAGGCTC TCGGGACGTG 
GTAGACGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACATCTTCCA CCTCCTCAAA GGAGTCCTCG TTGTCAGCTT CTCCGTCTCA GGACAAAAGG 
CTCCTCCTGG ACCCAGACAC AGCCATTGGG AAGCTCTATT GGCTCCAGCA GACCCTGGAG 
ACGGGCATCT GCAGCCTCAT GTCACTGGTC ACCATAGACT GGAGGTCTTA TGGATACATG 
GAAAGGCACA TCAACGAGAT CCGCACCGCA GTGGACAAAG TGGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAGGGG CGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACCGGTCCTC 
CTCAATAAAA TGAATCGGGA ACTCCAGAGA GTAGACGATT CCCACCAGAT CCTAAGCCAA 
ACCAGCCATG ATCTGAATGA ATGCAGCTGG TCCCTGAATG TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTGG CCAAGACAGT GCCCGACGAT 
GCCAAGCAAC TGACCACCAC CATCAGCACC TACGCAGAGA CTCTCTTTAG AGTAGATCCT 
GGCAATTCCC ATCTGAAGAA TGGGCCTGAC AGTATCACGA ACTCAATCGA GTACACACAT 
ACGGGCTCCC AGATGCAGCC ACTGCGCCCC GGTGACTACA AAGGCCAGGT TCACAATAAG 
CCATTGCCTC CTACTCTAAG CAAGGAGCAG CCACCAGACT GCAGCAGCAG TGACGGTTCC 
GAGAGGAGTT GGATGGACGA TTATGACTAT GTCCACCTGC AGGGCAAAGA GGAGTTTGAG 
AGACAACAGA AGGAACTCTT GGAAAAAGAG AACATCATGA AGCAGAGCAA GGCGCAGCTG 
GAGCATCATC AGCTGAGTCA GTTCCAGCTG TTAGAACAAG AAATCACCAA GCCCGTGGAG 
AATGACATTT CCAAATGGAA ACCCTCTCAG AGCCTCCCAA CCACAAACAA CAGTGTGGGT 
GCTCAGGATA GGCAATTGCT TTGCTTCTAC TATGACCAAT GCGAGACCCA TTTCATCTCC 
CTCCTCAATG CCATTGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAGCC CCCGCGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTC AGTGCACACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC CGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATTGTG ATGGCGACCA AGATGGCCGC CCTCCACTAC 
CCGAGCACTA CGGCTTTACA GGAAATGGTG CACCAAGTAA CAGACCTGTC CAGAAATGCT 
CAACTGTTCA AGCGTTCCTT GCTGGAGATG GCCACCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retinoic acid regulates Schwann cell migration via NEDD9 induction by transcriptional and post-translational mechanisms.
Biochimica et biophysica acta1863(7 Pt A)1510-8(2016 Jul)
Latasa MJ,Jim?nez-Lara AM,Cosgaya JM

CRK proteins selectively regulate T cell migration into inflamed tissues.
The Journal of clinical investigation125(3)1019-32(2015 Mar)
Huang Y,Clarke F,Karimi M,Roy NH,Williamson EK,Okumura M,Mochizuki K,Chen EJ,Park TJ,Debes GF,Zhang Y,Curran T,Kambayashi T,Burkhardt JK

Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis.
Proceedings of the National Academy of Sciences of the United States of America106(18)7473-8(2009 May)
Zheng P,Dean J

A novel Cas family member, HEPL, regulates FAK and cell spreading.
Molecular biology of the cell19(4)1627-36(2008 Apr)
Singh MK,Dadke D,Nicolas E,Serebriiskii IG,Apostolou S,Canutescu A,Egleston BL,Golemis EA

Nedd9 protein, a Cas-L homologue, is upregulated after transient global ischemia in rats: possible involvement of Nedd9 in the differentiation of neurons after ischemia.
Stroke36(11)2457-62(2005 Nov)
Sasaki T,Iwata S,Okano HJ,Urasaki Y,Hamada J,Tanaka H,Dang NH,Okano H,Morimoto C

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Nedd9 cDNA ORF clone, Rattus norvegicus(Norway rat)

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