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N4bp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol N4bp1
Entrez Gene ID 291921
Full Name Nedd4 binding protein 1
Synonyms RGD1305179
General protein information
Preferred Names

Nedd4 binding protein 1

Names

NEDD4-binding protein 1
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

19

19p11

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001305181.1 NP_001292110.1 NEDD4-binding protein 1
XM_017601208.1 XP_017456697.1 NEDD4-binding protein 1 isoform X1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) N4bp1 XP_214648.5
Pan troglodytes (chimpanzee) N4BP1 XP_520627.3
Canis lupus familiaris (dog) N4BP1 XP_005617644.1
N4BP1 XP_001109863.2
Gallus gallus (chicken) N4BP1 NP_001025741.1
Danio rerio (zebrafish) n4bp1 NP_956081.1
Mus musculus (house mouse) N4bp1 NP_085040.2
Homo sapiens (human) N4BP1 NP_694574.3
Bos taurus (cattle) N4BP1 NP_001178362.1
Xenopus tropicalis (tropical clawed frog) n4bp1 NP_001004870.1

Gene Ontology


Bibliography

Related articles in PubMed

N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies.
Sharma P, Murillas R, Zhang H, Kuehn MR
Journal of cell science123(Pt 8)1227-34(2010 Apr)

The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch.
Oberst A, Malatesta M, Aqeilan RI, Rossi M, Salomoni P, Murillas R, Sharma P, Kuehn MR, Oren M, Croce CM, Bernassola F, Melino G
Proceedings of the National Academy of Sciences of the United States of America104(27)11280-5(2007 Jul)

Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase.
Murillas R, Simms KS, Hatakeyama S, Weissman AM, Kuehn MR
The Journal of biological chemistry277(4)2897-907(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following N4bp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N4bp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa21010 NM_001305181.1
Latest version!		 Rattus norvegicus Nedd4 binding protein 1 (N4bp1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
ORa59393 XM_017601208.1
Latest version!		 Rattus norvegicus Nedd4 binding protein 1 (N4bp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa21010
Clone ID Related Accession (Same CDS sequence) NM_001305181.1
Accession Version NM_001305181.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Rattus norvegicus(Norway rat)
Product NEDD4-binding protein 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07043103.1. On or before Feb 27, 2015 this sequence version replaced XM_214648.8, XM_001068071.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCGGCCC GGGTAGTGCT GGATGAGTTC ACGGCGCCCG CCGAGAAGGC GGCGCTGCTG 
GAGCGGAGCC GCGGGCGCAT CGAGGCTCTG TTCGGCGTTG GCCTGGCGGT GCTCGGCGCC 
CTCGGGGCGG AGGAGCCGCT GCCTGCGCGC ATCTGGCTGC AGCTCCGCGG CGCGCAGGAG 
GCGGTGCACA GCGCCAAGGA GTACATCAAA GGGCTCTGCG AGCCTGAGCT GGAAGAAAGA 
GAGTGCTACC CCAAGGCCAT GCACTGCATT TTCGTTGGGG CCCAGAGCCT CTTTTTGAAG 
AGCTTAATTC AGGATACTTG TGCTGACCTC TGTGTTTTAG ATACTGGGCT TCTTGGCATT 
CGAGGAAGCG CTGAGGCTGT GGTCATGGCC AGAAGTCACA TTCAACAGTT TGTGAAGCTC 
TTTGAGAGTA AGGAGAGCCT CCCCAGTCAC CAGAAAGAGT CAGAAATTAA GCGGGAATTC 
AGACAGTTTG TTGAAGCCCA TGCAGACAGT TATACAATGG ACCTGTTGAT TCTGCCTACT 
TCCTTGAAGA AGGAATTACT GAGTCTCACA CAAGGGGAAG AAAATCTGTT TGAAACCGGA 
GATGATGATG TCATTACTGT TGGAGATGTA CGGCCACCAG AGTTCACACA GAGTACTGCC 
GCAGGACCGA GTATTGCCAG AGATGACATT GTTGTACAGG AAGAGTCCAG AAATAAAGCT 
AGGACTCCTG TTTCTGAGCT TACAAAACAA ATGGACACAG TCTTTTCTAG TTCACCAGAT 
GTGCTTTTTG TTCCAGTGAA TGGTCTAAGT CCAGATGAAG ATGCTCTTTC CAAAGACAGA 
GTTTGTCACA AGAGGAGGTC TTCTGATACA GAAGAAAGGC ATACAAAAAA GCAGTTTTCC 
TTAGAGAATG TTCCAGAAGG GGAGCTTTTG CAAGATGGTA AGGCATCAGC TGAAAATGAA 
GTCATCGACC TTTCTGATGC TGCTTCGAAC TCTACAAATC TAAGCCCCGA TGGAAAAGAC 
ACTACAGAAG AAATGGAGTA CAATATCCTT GTTAACTTCT TTAAAACCAT GGGCTATTCC 
CAAGAGATTG TTGAAAAGGT CATTAGGGAG TACGGGCCTT CTACAGAACC ACTCTTGCTT 
TTAGAAGAAA TTGAAAAAGA AAATAAAAGG CTCCAAGAAG ACAGAGACTT CCCACCGTGC 
ACTGTTTATC CAGAGGACAG TCAGAGCAGA AGTGCAGGAG GTAGGAGCAC GACAAACGAG 
CTCACAGCAG AGTCCACTCC AAAGAAAACA CAGAATCATA CAGAGCAAAC TATGGTGGAA 
AGATTCTCTC AGTTACCGTT CAAAGACTCT AAACAGTGTA CCTCAAATTG CAGAATTAAT 
TCTTTCAAAA CAGTGCCAGT AGGTCAGAAA CATGAAATCT GGGCTTCAAA GCAGAACTCA 
AATTGTACTG TGGACCTGGA AACTGATGGT GTCTTGGCCG CCTCTGCCAG CCCTAAAGAC 
GTCAGTTTTG TTTCAAGGGG AGCTTTAGGG CACCAGCAAA GAAACTCAGC TTTTCTTGAA 
AATGGCTTTC AACAGCAAGC AGAACCTTTG CTCCCAAATA ACGTGAAGCC TGCCTGTGAA 
AAGCGCTCTG GCGGCTGTGG CTCTCCTCAG CCTAAGCCAA ATTATCACCC CCCACTTTCT 
CCACCACTGC CGCCGCCCCA GCTGCTGCCT TCAGCTACTG AGGCTCGATT GGGAGGATCC 
TCTGATCATA TTGATTCCTC AGTTACGGGG GTTCAAAGAT TTCGAGATAC CTTAAAAATA 
CCGTACAAGC TGGAGTTAAA AAATGAGCCA GGAAGAGCAG ATTTGAAGCA CATTGTTATA 
GACGGGAGTA ATGTTGCCAT TACCCATGGT CTGAAAAAGT TCTTCAGTTG CCGTGGCATA 
GCCATTGCAG TTGAGTATTT TTGGAAGCTC GGCAACAGAA ACATCACTGT GTTTGTCCCG 
CAGTGGAGAA CAAGACGGGA TCCTAATATC ACAGAACAGC ACTTCTTAAC CCAGCTCCAG 
GAGCTTGGAA TATTATCTTT AACTCCTGCC CGGATGGTCT TTGGAGAAAG AATTGCTTCT 
CATGATGACA GGTTCCTCCT GCACTTAGCA GACAAGACCG GGGGCATCAT TGTAACCAAT 
GATAACTTCA GAGAGTTTGT GACCGAGTCC GTGTCCTGGA GAGAAATCAT TACGAAAAGA 
CTGCTTCAGT ACACATTCGT GGGGGACATA TTTATGGTTC CTGATGACCC TCTGGGAAGA 
AACGGACCTC GGCTGGAAGA ATTTCTTCGA AAGGAGGTCT TTCTTAGAGA CATGCAGCCG 
CTACTCAATG CCCTGCCAAG TGTGGGCACG TTTGACCCTG GCTTCCGGAG TCCCAGCACC 
CAGGTAGCCA ACACCAGCCA CCAGCCTCCA TCCCGGATCC AGACCTCCTC AAACCATTGG 
CTTCCTCAGC AGCCCCACTT CACACCCCTG GCCACGCTTC CCAGTATGCA GCAGAACCTT 
CCCGTGCCAG CACAGAGGTC TTCTGCAGAG ACCAGCGAGC TGAGGGAAGC TCTACTGAAG 
ATCTTCCCTG ACTCTGAGCA GAAGCTGAAG ATCGACCAGA TTCTGGTAGC TCATCCGTAC 
ATGAAAGACC TGAATGCCTT GTCTGCCCTG GTGTTGGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001292110.1
CDS1..2682
Translation

Target ORF information:

RefSeq Version NM_001305181.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Nedd4 binding protein 1 (N4bp1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001305181.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCGGCCC GGGTAGTGCT GGATGAGTTC ACGGCGCCCG CCGAGAAGGC GGCGCTGCTG 
GAGCGGAGCC GCGGGCGCAT CGAGGCTCTG TTCGGCGTTG GCCTGGCGGT GCTCGGCGCC 
CTCGGGGCGG AGGAGCCGCT GCCTGCGCGC ATCTGGCTGC AGCTCCGCGG CGCGCAGGAG 
GCGGTGCACA GCGCCAAGGA GTACATCAAA GGGCTCTGCG AGCCTGAGCT GGAAGAAAGA 
GAGTGCTACC CCAAGGCCAT GCACTGCATT TTCGTTGGGG CCCAGAGCCT CTTTTTGAAG 
AGCTTAATTC AGGATACTTG TGCTGACCTC TGTGTTTTAG ATACTGGGCT TCTTGGCATT 
CGAGGAAGCG CTGAGGCTGT GGTCATGGCC AGAAGTCACA TTCAACAGTT TGTGAAGCTC 
TTTGAGAGTA AGGAGAGCCT CCCCAGTCAC CAGAAAGAGT CAGAAATTAA GCGGGAATTC 
AGACAGTTTG TTGAAGCCCA TGCAGACAGT TATACAATGG ACCTGTTGAT TCTGCCTACT 
TCCTTGAAGA AGGAATTACT GAGTCTCACA CAAGGGGAAG AAAATCTGTT TGAAACCGGA 
GATGATGATG TCATTACTGT TGGAGATGTA CGGCCACCAG AGTTCACACA GAGTACTGCC 
GCAGGACCGA GTATTGCCAG AGATGACATT GTTGTACAGG AAGAGTCCAG AAATAAAGCT 
AGGACTCCTG TTTCTGAGCT TACAAAACAA ATGGACACAG TCTTTTCTAG TTCACCAGAT 
GTGCTTTTTG TTCCAGTGAA TGGTCTAAGT CCAGATGAAG ATGCTCTTTC CAAAGACAGA 
GTTTGTCACA AGAGGAGGTC TTCTGATACA GAAGAAAGGC ATACAAAAAA GCAGTTTTCC 
TTAGAGAATG TTCCAGAAGG GGAGCTTTTG CAAGATGGTA AGGCATCAGC TGAAAATGAA 
GTCATCGACC TTTCTGATGC TGCTTCGAAC TCTACAAATC TAAGCCCCGA TGGAAAAGAC 
ACTACAGAAG AAATGGAGTA CAATATCCTT GTTAACTTCT TTAAAACCAT GGGCTATTCC 
CAAGAGATTG TTGAAAAGGT CATTAGGGAG TACGGGCCTT CTACAGAACC ACTCTTGCTT 
TTAGAAGAAA TTGAAAAAGA AAATAAAAGG CTCCAAGAAG ACAGAGACTT CCCACCGTGC 
ACTGTTTATC CAGAGGACAG TCAGAGCAGA AGTGCAGGAG GTAGGAGCAC GACAAACGAG 
CTCACAGCAG AGTCCACTCC AAAGAAAACA CAGAATCATA CAGAGCAAAC TATGGTGGAA 
AGATTCTCTC AGTTACCGTT CAAAGACTCT AAACAGTGTA CCTCAAATTG CAGAATTAAT 
TCTTTCAAAA CAGTGCCAGT AGGTCAGAAA CATGAAATCT GGGCTTCAAA GCAGAACTCA 
AATTGTACTG TGGACCTGGA AACTGATGGT GTCTTGGCCG CCTCTGCCAG CCCTAAAGAC 
GTCAGTTTTG TTTCAAGGGG AGCTTTAGGG CACCAGCAAA GAAACTCAGC TTTTCTTGAA 
AATGGCTTTC AACAGCAAGC AGAACCTTTG CTCCCAAATA ACGTGAAGCC TGCCTGTGAA 
AAGCGCTCTG GCGGCTGTGG CTCTCCTCAG CCTAAGCCAA ATTATCACCC CCCACTTTCT 
CCACCACTGC CGCCGCCCCA GCTGCTGCCT TCAGCTACTG AGGCTCGATT GGGAGGATCC 
TCTGATCATA TTGATTCCTC AGTTACGGGG GTTCAAAGAT TTCGAGATAC CTTAAAAATA 
CCGTACAAGC TGGAGTTAAA AAATGAGCCA GGAAGAGCAG ATTTGAAGCA CATTGTTATA 
GACGGGAGTA ATGTTGCCAT TACCCATGGT CTGAAAAAGT TCTTCAGTTG CCGTGGCATA 
GCCATTGCAG TTGAGTATTT TTGGAAGCTC GGCAACAGAA ACATCACTGT GTTTGTCCCG 
CAGTGGAGAA CAAGACGGGA TCCTAATATC ACAGAACAGC ACTTCTTAAC CCAGCTCCAG 
GAGCTTGGAA TATTATCTTT AACTCCTGCC CGGATGGTCT TTGGAGAAAG AATTGCTTCT 
CATGATGACA GGTTCCTCCT GCACTTAGCA GACAAGACCG GGGGCATCAT TGTAACCAAT 
GATAACTTCA GAGAGTTTGT GACCGAGTCC GTGTCCTGGA GAGAAATCAT TACGAAAAGA 
CTGCTTCAGT ACACATTCGT GGGGGACATA TTTATGGTTC CTGATGACCC TCTGGGAAGA 
AACGGACCTC GGCTGGAAGA ATTTCTTCGA AAGGAGGTCT TTCTTAGAGA CATGCAGCCG 
CTACTCAATG CCCTGCCAAG TGTGGGCACG TTTGACCCTG GCTTCCGGAG TCCCAGCACC 
CAGGTAGCCA ACACCAGCCA CCAGCCTCCA TCCCGGATCC AGACCTCCTC AAACCATTGG 
CTTCCTCAGC AGCCCCACTT CACACCCCTG GCCACGCTTC CCAGTATGCA GCAGAACCTT 
CCCGTGCCAG CACAGAGGTC TTCTGCAGAG ACCAGCGAGC TGAGGGAAGC TCTACTGAAG 
ATCTTCCCTG ACTCTGAGCA GAAGCTGAAG ATCGACCAGA TTCTGGTAGC TCATCCGTAC 
ATGAAAGACC TGAATGCCTT GTCTGCCCTG GTGTTGGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa59393
Clone ID Related Accession (Same CDS sequence) XM_017601208.1
Accession Version XM_017601208.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2352bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product NEDD4-binding protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGGCCC GGGTAGTGCT GGATGAGTTC ACGGCGCCCG CCGAGAAGGC GGCGCTGCTG 
GAGCGGAGCC GCGGGCGCAT CGAGGCTCTG TTCGGCGTTG GCCTGGCGGT GCTCGGCGCC 
CTCGGGGCGG AGGAGCCGCT GCCTGCGCGC ATCTGGCTGC AGCTCCGCGG CGCGCAGGAG 
GCGGTGCACA GCGCCAAGGA GTACATCAAA GGGCTCTGCG AGCCTGAGCT GGAAGAAAGA 
GAGTGCTACC CCAAGGCCAT GCACTGCATT TTCGTTGGGG CCCAGAGCCT CTTTTTGAAG 
AGCTTAATTC AGGATACTTG TGCTGACCTC TGTGTTTTAG ATACTGGGCT TCTTGGCATT 
CGAGGAAGCG CTGAGGCTGT GGTCATGGCC AGAAGTCACA TTCAACAGTT TGTGAAGCTC 
TTTGAGAGTA AGGAGAGCCT CCCCAGTCAC CAGAAAGAGT CAGAAATTAA GCGGGAATTC 
AGACAGTTTG TTGAAGCCCA TGCAGACAGT TATACAATGG ACCTGTTGAT TCTGCCTACT 
TCCTTGAAGA AGGAATTACT GAGTCTCACA CAAGGGGAAG AAAATCTGTT TGAAACCGGA 
GATGATGATG TCATTACTGT TGGAGATGTA CGGCCACCAG AGTTCACACA GAGTACTGCC 
GCAGGACCGA GTATTGCCAG AGATGACATT GTTGTACAGG AAGAGTCCAG AAATAAAGCT 
AGGACTCCTG TTTCTGAGCT TACAAAACAA ATGGACACAG TCTTTTCTAG TTCACCAGAT 
GTGCTTTTTG TTCCAGTGAA TGGTCTAAGT CCAGATGAAG ATGCTCTTTC CAAAGACAGA 
GTTTGTCACA AGAGGAGGTC TTCTGATACA GAAGAAAGGC ATACAAAAAA GCAGTTTTCC 
TTAGAGAATG TTCCAGAAGG GGAGCTTTTG CAAGATGGTA AGGCATCAGC TGAAAATGAA 
GTCATCGACC TTTCTGATGC TGCTTCGAAC TCTACAAATC TAAGCCCCGA TGGAAAAGAC 
ACTACAGAAG AAATGGAGTA CAATATCCTT GTTAACTTCT TTAAAACCAT GGGCTATTCC 
CAAGAGATTG TTGAAAAGGT CATTAGGGAG TACGGGCCTT CTACAGAACC ACTCTTGCTT 
TTAGAAGAAA TTGAAAAAGA AAATAAAAGG CTCCAAGAAG ACAGAGACTT CCCACCGTGC 
ACTGTTTATC CAGAGGACAG TCAGAGCAGA AGTGCAGGAG GTAGGAGCAC GACAAACGAG 
CTCACAGCAG AGTCCACTCC AAAGAAAACA CAGAATCATA CAGAGCAAAC TATGGTGGAA 
AGATTCTCTC AGTTACCGTT CAAAGACTCT AAACAGTGTA CCTCAAATTG CAGAATTAAT 
TCTTTCAAAA CAGTGCCAGT AGGTCAGAAA CATGAAATCT GGGCTTCAAA GCAGAACTCA 
AATTGTACTG TGGACCTGGA AACTGATGGT GTCTTGGCCG CCTCTGCCAG CCCTAAAGAC 
GTCAGTTTTG TTTCAAGGGG AGCTTTAGGG CACCAGCAAA GAAACTCAGC TTTTCTTGAA 
AATGGCTTTC AACAGCAAGC AGAACCTTTG CTCCCAAATA ACGTGAAGCC TGCCTGTGAA 
AAGCGCTCTG GCGGCTGTGG CTCTCCTCAG CCTAAGCCAA ATTATCACCC CCCACTTTCT 
CCACCACTGC CGCCGCCCCA GCTGCTGCCT TCAGCTACTG AGGCTCGATT GGGAGGATCC 
TCTGATCATA TTGATTCCTC AGTTACGGGG GTTCAAAGAT TTCGAGATAC CTTAAAAATA 
CCGTACAAGC TGGAGTTAAA AAATGAGCCA GGAAGAGCAG ATTTGAAGCA CATTGTTATA 
GACGGGAGTA ATGTTGCCAT TACCCATGGT CTGAAAAAGT TCTTCAGTTG CCGTGGCATA 
GCCATTGCAG TTGAGTATTT TTGGAAGCTC GGCAACAGAA ACATCACTGT GTTTGTCCCG 
CAGTGGAGAA CAAGACGGGA TCCTAATATC ACAGAACAGC ACTTCTTAAC CCAGCTCCAG 
GAGCTTGGAA TATTATCTTT AACTCCTGCC CGGATGGTCT TTGGAGAAAG AATTGCTTCT 
CATGATGACA GGTTCCTCCT GCACTTAGCA GACAAGACCG GGGGCATCAT TGTAACCAAT 
GATAACTTCA GAGAGTTTGT GACCGAGTCC GTGTCCTGGA GAGAAATCAT TACGAAAAGA 
CTGCTTCAGT ACACATTCGT GGGGGACATA TTTATGGTTC CTGATGACCC TCTGGGAAGA 
AACGGACCTC GGCTGGAAGA ATTTCTTCGA AAGGAGGTCT TTCTTAGACC ATTTCTCTTG 
TCAGCAGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017456697.1
CDS219..2570
Translation

Target ORF information:

RefSeq Version XM_017601208.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Nedd4 binding protein 1 (N4bp1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017601208.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGGCCC GGGTAGTGCT GGATGAGTTC ACGGCGCCCG CCGAGAAGGC GGCGCTGCTG 
GAGCGGAGCC GCGGGCGCAT CGAGGCTCTG TTCGGCGTTG GCCTGGCGGT GCTCGGCGCC 
CTCGGGGCGG AGGAGCCGCT GCCTGCGCGC ATCTGGCTGC AGCTCCGCGG CGCGCAGGAG 
GCGGTGCACA GCGCCAAGGA GTACATCAAA GGGCTCTGCG AGCCTGAGCT GGAAGAAAGA 
GAGTGCTACC CCAAGGCCAT GCACTGCATT TTCGTTGGGG CCCAGAGCCT CTTTTTGAAG 
AGCTTAATTC AGGATACTTG TGCTGACCTC TGTGTTTTAG ATACTGGGCT TCTTGGCATT 
CGAGGAAGCG CTGAGGCTGT GGTCATGGCC AGAAGTCACA TTCAACAGTT TGTGAAGCTC 
TTTGAGAGTA AGGAGAGCCT CCCCAGTCAC CAGAAAGAGT CAGAAATTAA GCGGGAATTC 
AGACAGTTTG TTGAAGCCCA TGCAGACAGT TATACAATGG ACCTGTTGAT TCTGCCTACT 
TCCTTGAAGA AGGAATTACT GAGTCTCACA CAAGGGGAAG AAAATCTGTT TGAAACCGGA 
GATGATGATG TCATTACTGT TGGAGATGTA CGGCCACCAG AGTTCACACA GAGTACTGCC 
GCAGGACCGA GTATTGCCAG AGATGACATT GTTGTACAGG AAGAGTCCAG AAATAAAGCT 
AGGACTCCTG TTTCTGAGCT TACAAAACAA ATGGACACAG TCTTTTCTAG TTCACCAGAT 
GTGCTTTTTG TTCCAGTGAA TGGTCTAAGT CCAGATGAAG ATGCTCTTTC CAAAGACAGA 
GTTTGTCACA AGAGGAGGTC TTCTGATACA GAAGAAAGGC ATACAAAAAA GCAGTTTTCC 
TTAGAGAATG TTCCAGAAGG GGAGCTTTTG CAAGATGGTA AGGCATCAGC TGAAAATGAA 
GTCATCGACC TTTCTGATGC TGCTTCGAAC TCTACAAATC TAAGCCCCGA TGGAAAAGAC 
ACTACAGAAG AAATGGAGTA CAATATCCTT GTTAACTTCT TTAAAACCAT GGGCTATTCC 
CAAGAGATTG TTGAAAAGGT CATTAGGGAG TACGGGCCTT CTACAGAACC ACTCTTGCTT 
TTAGAAGAAA TTGAAAAAGA AAATAAAAGG CTCCAAGAAG ACAGAGACTT CCCACCGTGC 
ACTGTTTATC CAGAGGACAG TCAGAGCAGA AGTGCAGGAG GTAGGAGCAC GACAAACGAG 
CTCACAGCAG AGTCCACTCC AAAGAAAACA CAGAATCATA CAGAGCAAAC TATGGTGGAA 
AGATTCTCTC AGTTACCGTT CAAAGACTCT AAACAGTGTA CCTCAAATTG CAGAATTAAT 
TCTTTCAAAA CAGTGCCAGT AGGTCAGAAA CATGAAATCT GGGCTTCAAA GCAGAACTCA 
AATTGTACTG TGGACCTGGA AACTGATGGT GTCTTGGCCG CCTCTGCCAG CCCTAAAGAC 
GTCAGTTTTG TTTCAAGGGG AGCTTTAGGG CACCAGCAAA GAAACTCAGC TTTTCTTGAA 
AATGGCTTTC AACAGCAAGC AGAACCTTTG CTCCCAAATA ACGTGAAGCC TGCCTGTGAA 
AAGCGCTCTG GCGGCTGTGG CTCTCCTCAG CCTAAGCCAA ATTATCACCC CCCACTTTCT 
CCACCACTGC CGCCGCCCCA GCTGCTGCCT TCAGCTACTG AGGCTCGATT GGGAGGATCC 
TCTGATCATA TTGATTCCTC AGTTACGGGG GTTCAAAGAT TTCGAGATAC CTTAAAAATA 
CCGTACAAGC TGGAGTTAAA AAATGAGCCA GGAAGAGCAG ATTTGAAGCA CATTGTTATA 
GACGGGAGTA ATGTTGCCAT TACCCATGGT CTGAAAAAGT TCTTCAGTTG CCGTGGCATA 
GCCATTGCAG TTGAGTATTT TTGGAAGCTC GGCAACAGAA ACATCACTGT GTTTGTCCCG 
CAGTGGAGAA CAAGACGGGA TCCTAATATC ACAGAACAGC ACTTCTTAAC CCAGCTCCAG 
GAGCTTGGAA TATTATCTTT AACTCCTGCC CGGATGGTCT TTGGAGAAAG AATTGCTTCT 
CATGATGACA GGTTCCTCCT GCACTTAGCA GACAAGACCG GGGGCATCAT TGTAACCAAT 
GATAACTTCA GAGAGTTTGT GACCGAGTCC GTGTCCTGGA GAGAAATCAT TACGAAAAGA 
CTGCTTCAGT ACACATTCGT GGGGGACATA TTTATGGTTC CTGATGACCC TCTGGGAAGA 
AACGGACCTC GGCTGGAAGA ATTTCTTCGA AAGGAGGTCT TTCTTAGACC ATTTCTCTTG 
TCAGCAGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies.
Journal of cell science123(Pt 8)1227-34(2010 Apr)
Sharma P,Murillas R,Zhang H,Kuehn MR


book

The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch.
Proceedings of the National Academy of Sciences of the United States of America104(27)11280-5(2007 Jul)
Oberst A,Malatesta M,Aqeilan RI,Rossi M,Salomoni P,Murillas R,Sharma P,Kuehn MR,Oren M,Croce CM,Bernassola F,Melino G


book

Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase.
The Journal of biological chemistry277(4)2897-907(2002 Jan)
Murillas R,Simms KS,Hatakeyama S,Weissman AM,Kuehn MR