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Tufm cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Tufm
Entrez Gene ID 293481
Full Name Tu translation elongation factor, mitochondrial
General protein information
Preferred Names

Tu translation elongation factor, mitochondrial

Names

elongation factor Tu, mitochondrial
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

1

1q36

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006230224.1 XP_006230286.1 elongation factor Tu, mitochondrial isoform X1
XM_006230225.1 XP_006230287.1 elongation factor Tu, mitochondrial isoform X2
NM_001106295.1 NP_001099765.1 elongation factor Tu, mitochondrial precursor

Pathways from BioSystems


Homologies


TUFM XP_001103519.1
Homo sapiens (human) TUFM NP_003312.3
Saccharomyces cerevisiae (baker's yeast) TUF1 NP_014830.1
Kluyveromyces lactis KLLA0A04587g XP_451201.1
Kluyveromyces lactis KLLA0C05214g XP_452433.1
Eremothecium gossypii AGOS_AAR143W NP_982685.1
Magnaporthe oryzae (rice blast fungus) MGG_08162 XP_003715132.1
Canis lupus familiaris (dog) TUFM XP_536924.3
Bos taurus (cattle) TUFM NP_776632.1
Rattus norvegicus (Norway rat) Tufm NP_001099765.1
Danio rerio (zebrafish) tufm NP_001013523.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006996 XP_308774.4
Neurospora crassa NCU03737 XP_961513.1
Pan troglodytes (chimpanzee) TUFM XP_510904.2
Drosophila melanogaster (fruit fly) EfTuM NP_001163144.1
Schizosaccharomyces pombe (fission yeast) tuf1 NP_595746.1
Arabidopsis thaliana (thale cress) AT4G02930 NP_192202.1
Oryza sativa (rice) Os03g0851100 NP_001051912.1
Mus musculus (house mouse) Tufm NP_766333.1
Caenorhabditis elegans (roundworm) tufm-1 NP_497623.1

Gene Ontology


Bibliography

Related articles in PubMed

Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.
Zhang DX, Yan H, Hu JY, Zhang JP, Teng M, Tong DL, Xiang F, Zhang Q, Fang YD, Liang GP, Huang YS
Journal of proteomics77469-79(2012 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu in the rat liver: implications for the mitochondrial ribosome.
Weiser B, Gonye G, Sykora P, Crumm S, Cahill A
American journal of physiology. Gastrointestinal and liver physiology300(5)G815-22(2011 May)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the mitochondrial responses to severe burn, and indicate a pathway by which decreased EF-Tumt expression mediates oxidative damage in postburn myocardium.
Title: Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.

The following Tufm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tufm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa00764 NM_001106295.1
Latest version!		 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
ORa23409 XM_006230224.1
Latest version!		 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
ORa23410 XM_006230225.1
Latest version!		 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa00764
Clone ID Related Accession (Same CDS sequence) NM_001106295.1
Accession Version NM_001106295.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-18
Organism Rattus norvegicus(Norway rat)
Product elongation factor Tu, mitochondrial precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473956.2. On or before Oct 4, 2007 this sequence version replaced XM_001077188.1, XM_215069.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAGG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001099765.1
CDS347..1705
Translation

Target ORF information:

RefSeq Version NM_001106295.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001106295.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAGG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa23409
Clone ID Related Accession (Same CDS sequence) XM_006230224.1
Accession Version XM_006230224.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product elongation factor Tu, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TTGGTGCTCT GGTCCGAGGC 
TTAAAGCGAG AGGATTTGAG GCGTGGTTTG GTCATGGTCA AGCCAGGATC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAGGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGCCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACTTTGAG GGATGGCAAC 
AAGACCATTG GCACAGGCCT TGTCACTGAT GTACCAGCCA TGACCGAGGA GGACAAGAAC 
ATCAAATGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006230286.1
CDS342..1616
Translation

Target ORF information:

RefSeq Version XM_006230224.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006230224.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TTGGTGCTCT GGTCCGAGGC 
TTAAAGCGAG AGGATTTGAG GCGTGGTTTG GTCATGGTCA AGCCAGGATC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAGGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGCCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACTTTGAG GGATGGCAAC 
AAGACCATTG GCACAGGCCT TGTCACTGAT GTACCAGCCA TGACCGAGGA GGACAAGAAC 
ATCAAATGGA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa23410
Clone ID Related Accession (Same CDS sequence) XM_006230225.1
Accession Version XM_006230225.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1239bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product elongation factor Tu, mitochondrial isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006230287.1
CDS342..1580
Translation

Target ORF information:

RefSeq Version XM_006230225.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006230225.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.
Journal of proteomics77469-79(2012 Dec)
Zhang DX,Yan H,Hu JY,Zhang JP,Teng M,Tong DL,Xiang F,Zhang Q,Fang YD,Liang GP,Huang YS


book

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW


book

Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu in the rat liver: implications for the mitochondrial ribosome.
American journal of physiology. Gastrointestinal and liver physiology300(5)G815-22(2011 May)
Weiser B,Gonye G,Sykora P,Crumm S,Cahill A


book

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