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Skiv2l cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Skiv2l
Entrez Gene ID 294260
Full Name Ski2 like RNA helicase
General protein information
Preferred Names

Ski2 like RNA helicase

Names

helicase SKI2W
SKI2 homolog, superkiller viralicidic activity 2-like
superkiller viralicidic activity 2-like
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

20

20p12
Summary putative RNA helicase; member of a family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD); rat mouse and human homologs are all located within the MHC class III region [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006255923.2 XP_006255985.1 helicase SKI2W isoform X1
NM_213559.2 NP_998724.1 helicase SKI2W

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP003182 XP_312880.4
Homo sapiens (human) SKIV2L NP_008860.4
Danio rerio (zebrafish) skiv2l NP_001038366.1
Schizosaccharomyces pombe (fission yeast) SPCC550.03c NP_588095.1
Canis lupus familiaris (dog) SKIV2L XP_538841.2
Bos taurus (cattle) SKIV2L XP_003587743.1
Arabidopsis thaliana (thale cress) AT3G46960 NP_190280.5
Pan troglodytes (chimpanzee) SKIV2L XP_001160927.2
SKIV2L XP_001106475.2
Rattus norvegicus (Norway rat) Skiv2l NP_998724.1
Drosophila melanogaster (fruit fly) tst NP_524465.1
Caenorhabditis elegans (roundworm) skih-2 NP_502084.2
Saccharomyces cerevisiae (baker's yeast) SKI2 NP_013502.3
Xenopus tropicalis (tropical clawed frog) skiv2l XP_004917184.1
Mus musculus (house mouse) Skiv2l NP_067312.2
Eremothecium gossypii AGOS_AER067C NP_984927.2
Neurospora crassa NCU01857 XP_965488.1

Gene Ontology


Bibliography

Related articles in PubMed

The human PAF complex coordinates transcription with events downstream of RNA synthesis.
Zhu B, Mandal SS, Pham AD, Zheng Y, Erdjument-Bromage H, Batra SK, Tempst P, Reinberg D
Genes & development19(14)1668-73(2005 Jul)

The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes.
Qu X, Yang Z, Zhang S, Shen L, Dangel AW, Hughes JH, Redman KL, Wu LC, Yu CY
Nucleic acids research26(17)4068-77(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Skiv2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skiv2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa05659 NM_213559.2
Latest version!		 Rattus norvegicus Ski2 like RNA helicase (Skiv2l), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa39120 XM_006255923.2
Latest version!		 Rattus norvegicus Ski2 like RNA helicase (Skiv2l), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa05659
Clone ID Related Accession (Same CDS sequence) NM_213559.2
Accession Version NM_213559.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-28
Organism Rattus norvegicus(Norway rat)
Product helicase SKI2W
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883045.1. On Sep 28, 2009 this sequence version replaced NM_213559.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CCGAGCGACT CGTGCTGCCT CCCCCAGACC CCCTGAACCT GCCCCTTCGA 
GCTCTGGAAG TGGGGTGCAC TGGCCGTTGG GAGCTGCTAA ACGTGCCAGG GCCTCCAGAG 
AGTACTCTTC CCCATGGCCT CCCTCCCTGT GCCCCAGATC TGTGCCAGGA AGCAGAGCAG 
TTGTTTCTGT CGTCTCCGGC CTGGCTTCCT CTACACGGTG TGGAGCACTC AGCTCGAAAA 
TGGCAGAGGA AGACAGATCC CTGGTCGCTT CTGGCTGCTC TGGGGACTCC AGTCCCATCC 
GACCTTCAGG CCCAGAGACA CCCAACCACA GGCCATATAC TGGGCTATAA GGAGGTCCTG 
CTGGAGAACA CGAACCTGTC GGCCACCACC TCCTTGTCCC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CGCTATGGGG CAATCCGACA CAGTACCCTT TCTGGCCGGG TGGGATGGAC 
GAGCCCAGCA TAACTGATCT GAGTACTCGG GAGGAAGCCG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TCACCGTTCC ACCCGGCTTC AAGAAAGGGG TGGATTTTGC CCCCAAAGCT 
CCTGTGCCAG GTTTGCTCAG CCTCAGCCGT CTGCTGGAGC CTCTGGATCT GAGTGGAGGT 
GATGAGGGTG AGGGAGAGGC AGCAGGAGGT CCTAGAGGGG ATGCGGCCTC AGCCTCTCCC 
TCCAGCACTC CCCTGATCCG GGCAAGCAGC TTGGAGGACC TAGTGTTGAA GGAAGCTTCC 
ACAGTCGTAT CCACCCTGGA GCCCCTCAAA CCTCCACCTC AGGAACAGTG GGCTGTGCCT 
GTGGACGTCA CCTCCCCCGT GGGTGATTTT TACCGTCTCA TCCCTCAGCC GGCCTTCCAG 
TGGGCGTTCG AGCCAGATGT GTTTCAGAAG CAGGCCATCC TGCACTTGGA GCAGCACGAC 
TCTGTCTTTG TTGCAGCTCA CACATCCGCT GGGAAGACGG TGGTGGCCGA GTATGCCATT 
GCCCTGGCCC AGAAACACAT GACCCGTACC ATCTACACTT CTCCTATCAA AGCCCTGAGC 
AACCAGAAGT TTCGGGACTT CCGAAACACA TTCGGGGATG TGGGACTGCT CACGGGGGAT 
GTGCAGCTGC ACCCAGAGGC CTCCTGCCTC ATCATGACCA CGGAGATCCT TCGCTCCATG 
CTGTACAGTG GCTCAGACGT CATCCGAGAC CTGGAGTGGG TCATTTTTGA TGAAGTCCAC 
TATATCAATG ATGCTGAGCG TGGGGTCGTG TGGGAGGAGG TGCTCATTAT GCTCCCTGAG 
CACGTCTCCA TCATCCTGCT GAGTGCCACC GTCCCCAACG CCCTGGAGTT TGCTGACTGG 
ATCGGGAGGC TGAAGCGGCG GCAGATCTAT GTGATCAGCA CGGTGGCCCG TCCCGTCCCC 
TTGGAGCACT ATCTCTTCAC AGGAAACAGC CCCAAGACAC AGGGCGAACT CTTCCTTCTG 
CTGGACTCCC GAGGGGCCTT CCACACCAAG GGGTACTACG CTGCCGTGGA GGCCAAGAAA 
GAGAGGATGA GCAAACACGC CCAGACCTTC GGGGCCAAGC AGCCCACACA CCAGGGTGGG 
CCTGCGCAGG ACCGTGGGGT GTACCTGGCC TTGCTGGCTT CCCTCCGGAC CCGGGCTCAG 
TTGCCTGTGG TAGTGTTCAC CTTCTCCCGA GGCCGCTGTG ATGAACAGGC TTCAGGTCTC 
ACCTCCCTAG ACCTCACAAC AAGCTCAGAG AAGAGTGAGA TCCACCTCTT CCTGCAGCGC 
TGTCTTGCCC GGCTCCGAGG CTCTGACCGA CAGCTCCCCC AGGTCCTGCA CATGTCGGAG 
CTCCTCCGCC GAGGCCTGGG TGTGCACCAC AGCGGCATCC TGCCCATCCT TAAGGAGATT 
GTGGAGATGC TTTTCAGCCG GGGCCTAGTC AAGGTCCTGT TTGCCACCGA GACTTTTGCC 
ATGGGGGTAA ACATGCCAGC CAGGACAGTG GTGTTTGACT CCATGCGCAA ACATGATGGC 
TCCACCTTCC GGGACCTGCT GCCTGGAGAG TACGTGCAGA TGGCAGGCCG GGCGGGCCGG 
AGGGGCCTGG ACCCCACGGG CACCGTCATC CTTCTCTGTA AGGGCCGAGT GCCTGAGATG 
GCAGATCTAC ACCGCATGAT GATGGGGAAA CCATCACAGC TTCAGTCCCA GTTCCGCCTC 
ACATATACCA TGATCCTCAA CCTGCTGCGG GTGGACGCCC TCAGAGTGGA AGACATGATG 
AAGAGGAGCT TCTCAGAGTT CCCCTCCCGC AAGGACAGCA AGGCCCATGA GCAGGCTCTG 
GCTGACCTCA CCAAGAGGCT GGGGGCTTTG GAGGAGCCTG ATGTGACTGG TCAGTTGGCT 
GACCTGCCTG AGTATTACAG CTGGGCGGAG GAGCTGACAG AGACCCGGAA CATGATCCAG 
CGACGCATCA TGGAGTCTGT GAACGGGCTG AAATCTCTGT CAGTGGGAAG GGTGGTGGTT 
GTGAAGAATG AAGAGCATCA CAATGCGCTG GGTGTGATCC TGCAGGTCTC CTCAAACTCC 
ACTAGCCGAG TATTTACAAC CTTGGTCTTG TGTGATAAAC CTGCTGTGTC TGAGAACCCA 
AGGGACAAGG GGCCAGCCAC TCCAGATGTG CCCCACCCAG ATGACCTTGT AGGCTTCAAG 
TTGTTCCTGC CTGAAGGGCC CTGCGAGCAC ACTGTGGCCA AGCTCCAGCC AGGGGATGTG 
GCTGCCATCT CTACCAAGGT GCTCCGGGTG AATGGGGAGA AGATTTCTGA GGACTTCAGC 
AAGAGGCAGC AACCGAAATT CAGGAAGGAC CCTCCCCTCG CGGCCGTGAC CACTGCTGTT 
CAGGAGCTGC TGCGTCTGGC CCAGGCCTAT CCAGCAGGAC CCCCAACCCT CGACCCTATC 
AACGACCTGC AACTGAAGGA TGTGGCAGTG GTAGAAGGTG GGCTCCGGGC CCGGAAGCTG 
GAGGAGCTGA TCCGAGGCGC TCAGTGTGTA CACAGCCCCC GGTTTCCTGC CCAGTATGTG 
AAGCTACAGG AGCGAATGCA GATTCAGAAG GAAATGGAGA GGCTGCGCTT CCTCCTGTCT 
GACCAGTCGC TGTTGCTGCT CCCCGAGTAC CACCAGCGTG TAGAGGTGCT TCGGACCCTG 
GGCTATGTGG ATGAGGCTGG CACAGTGAAG CTGGCGGGCC GGGTGGCCTG TGCCATGAGC 
AGCCATGAGC TGCTCCTCAC TGAACTCATG TTTGACAATG CACTGAGTGC TCTGCGACCA 
GAGGAAATAG CAGCTCTGCT CTCTGGTCTG GTGTGCCAGA GCCCCGGAGA CCCTGGGGAT 
CAGCTGCCAA GCACCCTCAA ACAGGGGGTA GAACGCGTCA AAGCTGTGGC CAAGCGGATT 
GGTGAGGTTC AGGTGGCCTG CGGCCTGAAC CAGACGGTGG AGGAATTTGT GGGGGAGCTG 
AATTTTGGGC TGGTGGAAGT TGTTTATGAG TGGGCTCGGG GTATGCCCTT CTCCGAGTTG 
GCAGGGCTCT CGGGGACCCC CGAAGGCTTG GTTGTCCGCT GTATCCAGCG CCTGGCTGAG 
ATGTGCCGCT CACTTCGAGG GGCGGCCCGT TTGGTAGGAG AGCCTGTGTT AGGGGCCAAG 
ATGGAGACGG CAGCCACCTT GCTCCGGAGG GACATCGTCT TCGCAGCCAG CCTCTATACC 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_998724.1
CDS31..3756
Translation

Target ORF information:

RefSeq Version NM_213559.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Ski2 like RNA helicase (Skiv2l), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_213559.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CCGAGCGACT CGTGCTGCCT CCCCCAGACC CCCTGAACCT GCCCCTTCGA 
GCTCTGGAAG TGGGGTGCAC TGGCCGTTGG GAGCTGCTAA ACGTGCCAGG GCCTCCAGAG 
AGTACTCTTC CCCATGGCCT CCCTCCCTGT GCCCCAGATC TGTGCCAGGA AGCAGAGCAG 
TTGTTTCTGT CGTCTCCGGC CTGGCTTCCT CTACACGGTG TGGAGCACTC AGCTCGAAAA 
TGGCAGAGGA AGACAGATCC CTGGTCGCTT CTGGCTGCTC TGGGGACTCC AGTCCCATCC 
GACCTTCAGG CCCAGAGACA CCCAACCACA GGCCATATAC TGGGCTATAA GGAGGTCCTG 
CTGGAGAACA CGAACCTGTC GGCCACCACC TCCTTGTCCC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CGCTATGGGG CAATCCGACA CAGTACCCTT TCTGGCCGGG TGGGATGGAC 
GAGCCCAGCA TAACTGATCT GAGTACTCGG GAGGAAGCCG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TCACCGTTCC ACCCGGCTTC AAGAAAGGGG TGGATTTTGC CCCCAAAGCT 
CCTGTGCCAG GTTTGCTCAG CCTCAGCCGT CTGCTGGAGC CTCTGGATCT GAGTGGAGGT 
GATGAGGGTG AGGGAGAGGC AGCAGGAGGT CCTAGAGGGG ATGCGGCCTC AGCCTCTCCC 
TCCAGCACTC CCCTGATCCG GGCAAGCAGC TTGGAGGACC TAGTGTTGAA GGAAGCTTCC 
ACAGTCGTAT CCACCCTGGA GCCCCTCAAA CCTCCACCTC AGGAACAGTG GGCTGTGCCT 
GTGGACGTCA CCTCCCCCGT GGGTGATTTT TACCGTCTCA TCCCTCAGCC GGCCTTCCAG 
TGGGCGTTCG AGCCAGATGT GTTTCAGAAG CAGGCCATCC TGCACTTGGA GCAGCACGAC 
TCTGTCTTTG TTGCAGCTCA CACATCCGCT GGGAAGACGG TGGTGGCCGA GTATGCCATT 
GCCCTGGCCC AGAAACACAT GACCCGTACC ATCTACACTT CTCCTATCAA AGCCCTGAGC 
AACCAGAAGT TTCGGGACTT CCGAAACACA TTCGGGGATG TGGGACTGCT CACGGGGGAT 
GTGCAGCTGC ACCCAGAGGC CTCCTGCCTC ATCATGACCA CGGAGATCCT TCGCTCCATG 
CTGTACAGTG GCTCAGACGT CATCCGAGAC CTGGAGTGGG TCATTTTTGA TGAAGTCCAC 
TATATCAATG ATGCTGAGCG TGGGGTCGTG TGGGAGGAGG TGCTCATTAT GCTCCCTGAG 
CACGTCTCCA TCATCCTGCT GAGTGCCACC GTCCCCAACG CCCTGGAGTT TGCTGACTGG 
ATCGGGAGGC TGAAGCGGCG GCAGATCTAT GTGATCAGCA CGGTGGCCCG TCCCGTCCCC 
TTGGAGCACT ATCTCTTCAC AGGAAACAGC CCCAAGACAC AGGGCGAACT CTTCCTTCTG 
CTGGACTCCC GAGGGGCCTT CCACACCAAG GGGTACTACG CTGCCGTGGA GGCCAAGAAA 
GAGAGGATGA GCAAACACGC CCAGACCTTC GGGGCCAAGC AGCCCACACA CCAGGGTGGG 
CCTGCGCAGG ACCGTGGGGT GTACCTGGCC TTGCTGGCTT CCCTCCGGAC CCGGGCTCAG 
TTGCCTGTGG TAGTGTTCAC CTTCTCCCGA GGCCGCTGTG ATGAACAGGC TTCAGGTCTC 
ACCTCCCTAG ACCTCACAAC AAGCTCAGAG AAGAGTGAGA TCCACCTCTT CCTGCAGCGC 
TGTCTTGCCC GGCTCCGAGG CTCTGACCGA CAGCTCCCCC AGGTCCTGCA CATGTCGGAG 
CTCCTCCGCC GAGGCCTGGG TGTGCACCAC AGCGGCATCC TGCCCATCCT TAAGGAGATT 
GTGGAGATGC TTTTCAGCCG GGGCCTAGTC AAGGTCCTGT TTGCCACCGA GACTTTTGCC 
ATGGGGGTAA ACATGCCAGC CAGGACAGTG GTGTTTGACT CCATGCGCAA ACATGATGGC 
TCCACCTTCC GGGACCTGCT GCCTGGAGAG TACGTGCAGA TGGCAGGCCG GGCGGGCCGG 
AGGGGCCTGG ACCCCACGGG CACCGTCATC CTTCTCTGTA AGGGCCGAGT GCCTGAGATG 
GCAGATCTAC ACCGCATGAT GATGGGGAAA CCATCACAGC TTCAGTCCCA GTTCCGCCTC 
ACATATACCA TGATCCTCAA CCTGCTGCGG GTGGACGCCC TCAGAGTGGA AGACATGATG 
AAGAGGAGCT TCTCAGAGTT CCCCTCCCGC AAGGACAGCA AGGCCCATGA GCAGGCTCTG 
GCTGACCTCA CCAAGAGGCT GGGGGCTTTG GAGGAGCCTG ATGTGACTGG TCAGTTGGCT 
GACCTGCCTG AGTATTACAG CTGGGCGGAG GAGCTGACAG AGACCCGGAA CATGATCCAG 
CGACGCATCA TGGAGTCTGT GAACGGGCTG AAATCTCTGT CAGTGGGAAG GGTGGTGGTT 
GTGAAGAATG AAGAGCATCA CAATGCGCTG GGTGTGATCC TGCAGGTCTC CTCAAACTCC 
ACTAGCCGAG TATTTACAAC CTTGGTCTTG TGTGATAAAC CTGCTGTGTC TGAGAACCCA 
AGGGACAAGG GGCCAGCCAC TCCAGATGTG CCCCACCCAG ATGACCTTGT AGGCTTCAAG 
TTGTTCCTGC CTGAAGGGCC CTGCGAGCAC ACTGTGGCCA AGCTCCAGCC AGGGGATGTG 
GCTGCCATCT CTACCAAGGT GCTCCGGGTG AATGGGGAGA AGATTTCTGA GGACTTCAGC 
AAGAGGCAGC AACCGAAATT CAGGAAGGAC CCTCCCCTCG CGGCCGTGAC CACTGCTGTT 
CAGGAGCTGC TGCGTCTGGC CCAGGCCTAT CCAGCAGGAC CCCCAACCCT CGACCCTATC 
AACGACCTGC AACTGAAGGA TGTGGCAGTG GTAGAAGGTG GGCTCCGGGC CCGGAAGCTG 
GAGGAGCTGA TCCGAGGCGC TCAGTGTGTA CACAGCCCCC GGTTTCCTGC CCAGTATGTG 
AAGCTACAGG AGCGAATGCA GATTCAGAAG GAAATGGAGA GGCTGCGCTT CCTCCTGTCT 
GACCAGTCGC TGTTGCTGCT CCCCGAGTAC CACCAGCGTG TAGAGGTGCT TCGGACCCTG 
GGCTATGTGG ATGAGGCTGG CACAGTGAAG CTGGCGGGCC GGGTGGCCTG TGCCATGAGC 
AGCCATGAGC TGCTCCTCAC TGAACTCATG TTTGACAATG CACTGAGTGC TCTGCGACCA 
GAGGAAATAG CAGCTCTGCT CTCTGGTCTG GTGTGCCAGA GCCCCGGAGA CCCTGGGGAT 
CAGCTGCCAA GCACCCTCAA ACAGGGGGTA GAACGCGTCA AAGCTGTGGC CAAGCGGATT 
GGTGAGGTTC AGGTGGCCTG CGGCCTGAAC CAGACGGTGG AGGAATTTGT GGGGGAGCTG 
AATTTTGGGC TGGTGGAAGT TGTTTATGAG TGGGCTCGGG GTATGCCCTT CTCCGAGTTG 
GCAGGGCTCT CGGGGACCCC CGAAGGCTTG GTTGTCCGCT GTATCCAGCG CCTGGCTGAG 
ATGTGCCGCT CACTTCGAGG GGCGGCCCGT TTGGTAGGAG AGCCTGTGTT AGGGGCCAAG 
ATGGAGACGG CAGCCACCTT GCTCCGGAGG GACATCGTCT TCGCAGCCAG CCTCTATACC 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa39120
Clone ID Related Accession (Same CDS sequence) XM_006255923.2
Accession Version XM_006255923.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product helicase SKI2W isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CCGAGCGACT CGTGCTGCCT CCCCCAGACC CCCTGAACCT GCCCCTTCGA 
GCTCTGGAAG TGGGGTGCAC TGGCCGTTGG GAGCTGCTAA ACGTGCCAGG GCCTCCAGAG 
AGTACTCTTC CCCATGGCCT CCCTCCCTGT GCCCCAGATC TGTGCCAGGA AGCAGAGCAG 
TTGTTTCTGT CGTCTCCGGC CTGGCTTCCT CTACACGGTG TGGAGCACTC AGCTCGAAAA 
TGGCAGAGGA AGACAGATCC CTGGTCGCTT CTGGCTGCTC TGGGGACTCC AGTCCCATCC 
GACCTTCAGG CCCAGAGACA CCCAACCACA GGCCATATAC TGGGCTATAA GGAGGTCCTG 
CTGGAGAACA CGAACCTGTC GGCCACCACC TCCTTGTCCC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CGCTATGGGG CAATCCGACA CAGTACCCTT TCTGGCCGGG TGGGATGGAC 
GAGCCCAGCA TAACTGATCT GAGTACTCGG GAGGAAGCCG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TCACCGTTCC ACCCGGCTTC AAGAAAGGGG TGGATTTTGC CCCCAAAGAT 
CACCCAGCTC CTGTGCCAGG TTTGCTCAGC CTCAGCCGTC TGCTGGAGCC TCTGGATCTG 
AGTGGAGGTG ATGAGGGTGA GGGAGAGGCA GCAGGAGGTC CTAGAGGGGA TGCGGCCTCA 
GCCTCTCCCT CCAGCACTCC CCTGATCCGG GCAAGCAGCT TGGAGGACCT AGTGTTGAAG 
GAAGCTTCCA CAGTCGTATC CACCCTGGAG CCCCTCAAAC CTCCACCTCA GGAACAGTGG 
GCTGTGCCTG TGGACGTCAC CTCCCCCGTG GGTGATTTTT ACCGTCTCAT CCCTCAGCCG 
GCCTTCCAGT GGGCGTTCGA GCCAGATGTG TTTCAGAAGC AGGCCATCCT GCACTTGGAG 
CAGCACGACT CTGTCTTTGT TGCAGCTCAC ACATCCGCTG GGAAGACGGT GGTGGCCGAG 
TATGCCATTG CCCTGGCCCA GAAACACATG ACCCGTACCA TCTACACTTC TCCTATCAAA 
GCCCTGAGCA ACCAGAAGTT TCGGGACTTC CGAAACACAT TCGGGGATGT GGGACTGCTC 
ACGGGGGATG TGCAGCTGCA CCCAGAGGCC TCCTGCCTCA TCATGACCAC GGAGATCCTT 
CGCTCCATGC TGTACAGTGG CTCAGACGTC ATCCGAGACC TGGAGTGGGT CATTTTTGAT 
GAAGTCCACT ATATCAATGA TGCTGAGCGT GGGGTCGTGT GGGAGGAGGT GCTCATTATG 
CTCCCTGAGC ACGTCTCCAT CATCCTGCTG AGTGCCACCG TCCCCAACGC CCTGGAGTTT 
GCTGACTGGA TCGGGAGGCT GAAGCGGCGG CAGATCTATG TGATCAGCAC GGTGGCCCGT 
CCCGTCCCCT TGGAGCACTA TCTCTTCACA GGAAACAGCC CCAAGACACA GGGCGAACTC 
TTCCTTCTGC TGGACTCCCG AGGGGCCTTC CACACCAAGG GGTACTACGC TGCCGTGGAG 
GCCAAGAAAG AGAGGATGAG CAAACACGCC CAGACCTTCG GGGCCAAGCA GCCCACACAC 
CAGGGTGGGC CTGCGCAGGA CCGTGGGGTG TACCTGGCCT TGCTGGCTTC CCTCCGGACC 
CGGGCTCAGT TGCCTGTGGT AGTGTTCACC TTCTCCCGAG GCCGCTGTGA TGAACAGGCT 
TCAGGTCTCA CCTCCCTAGA CCTCACAACA AGCTCAGAGA AGAGTGAGAT CCACCTCTTC 
CTGCAGCGCT GTCTTGCCCG GCTCCGAGGC TCTGACCGAC AGCTCCCCCA GGTCCTGCAC 
ATGTCGGAGC TCCTCCGCCG AGGCCTGGGT GTGCACCACA GCGGCATCCT GCCCATCCTT 
AAGGAGATTG TGGAGATGCT TTTCAGCCGG GGCCTAGTCA AGGTCCTGTT TGCCACCGAG 
ACTTTTGCCA TGGGGGTAAA CATGCCAGCC AGGACAGTGG TGTTTGACTC CATGCGCAAA 
CATGATGGCT CCACCTTCCG GGACCTGCTG CCTGGAGAGT ACGTGCAGAT GGCAGGCCGG 
GCGGGCCGGA GGGGCCTGGA CCCCACGGGC ACCGTCATCC TTCTCTGTAA GGGCCGAGTG 
CCTGAGATGG CAGATCTACA CCGCATGATG ATGGGGAAAC CATCACAGCT TCAGTCCCAG 
TTCCGCCTCA CATATACCAT GATCCTCAAC CTGCTGCGGG TGGACGCCCT CAGAGTGGAA 
GACATGATGA AGAGGAGCTT CTCAGAGTTC CCCTCCCGCA AGGACAGCAA GGCCCATGAG 
CAGGCTCTGG CTGACCTCAC CAAGAGGCTG GGGGCTTTGG AGGAGCCTGA TGTGACTGGT 
CAGTTGGCTG ACCTGCCTGA GTATTACAGC TGGGCGGAGG AGCTGACAGA GACCCGGAAC 
ATGATCCAGC GACGCATCAT GGAGTCTGTG AACGGGCTGA AATCTCTGTC AGTGGGAAGG 
GTGGTGGTTG TGAAGAATGA AGAGCATCAC AATGCGCTGG GTGTGATCCT GCAGGTCTCC 
TCAAACTCCA CTAGCCGAGT ATTTACAACC TTGGTCTTGT GTGATAAACC TGCTGTGTCT 
GAGAACCCAA GGGACAAGGG GCCAGCCACT CCAGATGTGC CCCACCCAGA TGACCTTGTA 
GGCTTCAAGT TGTTCCTGCC TGAAGGGCCC TGCGAGCACA CTGTGGCCAA GCTCCAGCCA 
GGGGATGTGG CTGCCATCTC TACCAAGGTG CTCCGGGTGA ATGGGGAGAA GATTTCTGAG 
GACTTCAGCA AGAGGCAGCA ACCGAAATTC AGGAAGGACC CTCCCCTCGC GGCCGTGACC 
ACTGCTGTTC AGGAGCTGCT GCGTCTGGCC CAGGCCTATC CAGCAGGACC CCCAACCCTC 
GACCCTATCA ACGACCTGCA ACTGAAGGAT GTGGCAGTGG TAGAAGGTGG GCTCCGGGCC 
CGGAAGCTGG AGGAGCTGAT CCGAGGCGCT CAGTGTGTAC ACAGCCCCCG GTTTCCTGCC 
CAGTATGTGA AGCTACAGGA GCGAATGCAG ATTCAGAAGG AAATGGAGAG GCTGCGCTTC 
CTCCTGTCTG ACCAGTCGCT GTTGCTGCTC CCCGAGTACC ACCAGCGTGT AGAGGTGCTT 
CGGACCCTGG GCTATGTGGA TGAGGCTGGC ACAGTGAAGC TGGCGGGCCG GGTGGCCTGT 
GCCATGAGCA GCCATGAGCT GCTCCTCACT GAACTCATGT TTGACAATGC ACTGAGTGCT 
CTGCGACCAG AGGAAATAGC AGCTCTGCTC TCTGGTCTGG TGTGCCAGAG CCCCGGAGAC 
CCTGGGGATC AGCTGCCAAG CACCCTCAAA CAGGGGGTAG AACGCGTCAA AGCTGTGGCC 
AAGCGGATTG GTGAGGTTCA GGTGGCCTGC GGCCTGAACC AGACGGTGGA GGAATTTGTG 
GGGGAGCTGA ATTTTGGGCT GGTGGAAGTT GTTTATGAGT GGGCTCGGGG TATGCCCTTC 
TCCGAGTTGG CAGGGCTCTC GGGGACCCCC GAAGGCTTGG TTGTCCGCTG TATCCAGCGC 
CTGGCTGAGA TGTGCCGCTC ACTTCGAGGG GCGGCCCGTT TGGTAGGAGA GCCTGTGTTA 
GGGGCCAAGA TGGAGACGGC AGCCACCTTG CTCCGGAGGG ACATCGTCTT CGCAGCCAGC 
CTCTATACCC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006255985.1
CDS90..3824
Translation

Target ORF information:

RefSeq Version XM_006255923.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus Ski2 like RNA helicase (Skiv2l), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006255923.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGATGGAGA CCGAGCGACT CGTGCTGCCT CCCCCAGACC CCCTGAACCT GCCCCTTCGA 
GCTCTGGAAG TGGGGTGCAC TGGCCGTTGG GAGCTGCTAA ACGTGCCAGG GCCTCCAGAG 
AGTACTCTTC CCCATGGCCT CCCTCCCTGT GCCCCAGATC TGTGCCAGGA AGCAGAGCAG 
TTGTTTCTGT CGTCTCCGGC CTGGCTTCCT CTACACGGTG TGGAGCACTC AGCTCGAAAA 
TGGCAGAGGA AGACAGATCC CTGGTCGCTT CTGGCTGCTC TGGGGACTCC AGTCCCATCC 
GACCTTCAGG CCCAGAGACA CCCAACCACA GGCCATATAC TGGGCTATAA GGAGGTCCTG 
CTGGAGAACA CGAACCTGTC GGCCACCACC TCCTTGTCCC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CGCTATGGGG CAATCCGACA CAGTACCCTT TCTGGCCGGG TGGGATGGAC 
GAGCCCAGCA TAACTGATCT GAGTACTCGG GAGGAAGCCG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TCACCGTTCC ACCCGGCTTC AAGAAAGGGG TGGATTTTGC CCCCAAAGAT 
CACCCAGCTC CTGTGCCAGG TTTGCTCAGC CTCAGCCGTC TGCTGGAGCC TCTGGATCTG 
AGTGGAGGTG ATGAGGGTGA GGGAGAGGCA GCAGGAGGTC CTAGAGGGGA TGCGGCCTCA 
GCCTCTCCCT CCAGCACTCC CCTGATCCGG GCAAGCAGCT TGGAGGACCT AGTGTTGAAG 
GAAGCTTCCA CAGTCGTATC CACCCTGGAG CCCCTCAAAC CTCCACCTCA GGAACAGTGG 
GCTGTGCCTG TGGACGTCAC CTCCCCCGTG GGTGATTTTT ACCGTCTCAT CCCTCAGCCG 
GCCTTCCAGT GGGCGTTCGA GCCAGATGTG TTTCAGAAGC AGGCCATCCT GCACTTGGAG 
CAGCACGACT CTGTCTTTGT TGCAGCTCAC ACATCCGCTG GGAAGACGGT GGTGGCCGAG 
TATGCCATTG CCCTGGCCCA GAAACACATG ACCCGTACCA TCTACACTTC TCCTATCAAA 
GCCCTGAGCA ACCAGAAGTT TCGGGACTTC CGAAACACAT TCGGGGATGT GGGACTGCTC 
ACGGGGGATG TGCAGCTGCA CCCAGAGGCC TCCTGCCTCA TCATGACCAC GGAGATCCTT 
CGCTCCATGC TGTACAGTGG CTCAGACGTC ATCCGAGACC TGGAGTGGGT CATTTTTGAT 
GAAGTCCACT ATATCAATGA TGCTGAGCGT GGGGTCGTGT GGGAGGAGGT GCTCATTATG 
CTCCCTGAGC ACGTCTCCAT CATCCTGCTG AGTGCCACCG TCCCCAACGC CCTGGAGTTT 
GCTGACTGGA TCGGGAGGCT GAAGCGGCGG CAGATCTATG TGATCAGCAC GGTGGCCCGT 
CCCGTCCCCT TGGAGCACTA TCTCTTCACA GGAAACAGCC CCAAGACACA GGGCGAACTC 
TTCCTTCTGC TGGACTCCCG AGGGGCCTTC CACACCAAGG GGTACTACGC TGCCGTGGAG 
GCCAAGAAAG AGAGGATGAG CAAACACGCC CAGACCTTCG GGGCCAAGCA GCCCACACAC 
CAGGGTGGGC CTGCGCAGGA CCGTGGGGTG TACCTGGCCT TGCTGGCTTC CCTCCGGACC 
CGGGCTCAGT TGCCTGTGGT AGTGTTCACC TTCTCCCGAG GCCGCTGTGA TGAACAGGCT 
TCAGGTCTCA CCTCCCTAGA CCTCACAACA AGCTCAGAGA AGAGTGAGAT CCACCTCTTC 
CTGCAGCGCT GTCTTGCCCG GCTCCGAGGC TCTGACCGAC AGCTCCCCCA GGTCCTGCAC 
ATGTCGGAGC TCCTCCGCCG AGGCCTGGGT GTGCACCACA GCGGCATCCT GCCCATCCTT 
AAGGAGATTG TGGAGATGCT TTTCAGCCGG GGCCTAGTCA AGGTCCTGTT TGCCACCGAG 
ACTTTTGCCA TGGGGGTAAA CATGCCAGCC AGGACAGTGG TGTTTGACTC CATGCGCAAA 
CATGATGGCT CCACCTTCCG GGACCTGCTG CCTGGAGAGT ACGTGCAGAT GGCAGGCCGG 
GCGGGCCGGA GGGGCCTGGA CCCCACGGGC ACCGTCATCC TTCTCTGTAA GGGCCGAGTG 
CCTGAGATGG CAGATCTACA CCGCATGATG ATGGGGAAAC CATCACAGCT TCAGTCCCAG 
TTCCGCCTCA CATATACCAT GATCCTCAAC CTGCTGCGGG TGGACGCCCT CAGAGTGGAA 
GACATGATGA AGAGGAGCTT CTCAGAGTTC CCCTCCCGCA AGGACAGCAA GGCCCATGAG 
CAGGCTCTGG CTGACCTCAC CAAGAGGCTG GGGGCTTTGG AGGAGCCTGA TGTGACTGGT 
CAGTTGGCTG ACCTGCCTGA GTATTACAGC TGGGCGGAGG AGCTGACAGA GACCCGGAAC 
ATGATCCAGC GACGCATCAT GGAGTCTGTG AACGGGCTGA AATCTCTGTC AGTGGGAAGG 
GTGGTGGTTG TGAAGAATGA AGAGCATCAC AATGCGCTGG GTGTGATCCT GCAGGTCTCC 
TCAAACTCCA CTAGCCGAGT ATTTACAACC TTGGTCTTGT GTGATAAACC TGCTGTGTCT 
GAGAACCCAA GGGACAAGGG GCCAGCCACT CCAGATGTGC CCCACCCAGA TGACCTTGTA 
GGCTTCAAGT TGTTCCTGCC TGAAGGGCCC TGCGAGCACA CTGTGGCCAA GCTCCAGCCA 
GGGGATGTGG CTGCCATCTC TACCAAGGTG CTCCGGGTGA ATGGGGAGAA GATTTCTGAG 
GACTTCAGCA AGAGGCAGCA ACCGAAATTC AGGAAGGACC CTCCCCTCGC GGCCGTGACC 
ACTGCTGTTC AGGAGCTGCT GCGTCTGGCC CAGGCCTATC CAGCAGGACC CCCAACCCTC 
GACCCTATCA ACGACCTGCA ACTGAAGGAT GTGGCAGTGG TAGAAGGTGG GCTCCGGGCC 
CGGAAGCTGG AGGAGCTGAT CCGAGGCGCT CAGTGTGTAC ACAGCCCCCG GTTTCCTGCC 
CAGTATGTGA AGCTACAGGA GCGAATGCAG ATTCAGAAGG AAATGGAGAG GCTGCGCTTC 
CTCCTGTCTG ACCAGTCGCT GTTGCTGCTC CCCGAGTACC ACCAGCGTGT AGAGGTGCTT 
CGGACCCTGG GCTATGTGGA TGAGGCTGGC ACAGTGAAGC TGGCGGGCCG GGTGGCCTGT 
GCCATGAGCA GCCATGAGCT GCTCCTCACT GAACTCATGT TTGACAATGC ACTGAGTGCT 
CTGCGACCAG AGGAAATAGC AGCTCTGCTC TCTGGTCTGG TGTGCCAGAG CCCCGGAGAC 
CCTGGGGATC AGCTGCCAAG CACCCTCAAA CAGGGGGTAG AACGCGTCAA AGCTGTGGCC 
AAGCGGATTG GTGAGGTTCA GGTGGCCTGC GGCCTGAACC AGACGGTGGA GGAATTTGTG 
GGGGAGCTGA ATTTTGGGCT GGTGGAAGTT GTTTATGAGT GGGCTCGGGG TATGCCCTTC 
TCCGAGTTGG CAGGGCTCTC GGGGACCCCC GAAGGCTTGG TTGTCCGCTG TATCCAGCGC 
CTGGCTGAGA TGTGCCGCTC ACTTCGAGGG GCGGCCCGTT TGGTAGGAGA GCCTGTGTTA 
GGGGCCAAGA TGGAGACGGC AGCCACCTTG CTCCGGAGGG ACATCGTCTT CGCAGCCAGC 
CTCTATACCC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The human PAF complex coordinates transcription with events downstream of RNA synthesis.
Genes & development19(14)1668-73(2005 Jul)
Zhu B,Mandal SS,Pham AD,Zheng Y,Erdjument-Bromage H,Batra SK,Tempst P,Reinberg D


book

The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes.
Nucleic acids research26(17)4068-77(1998 Sep)
Qu X,Yang Z,Zhang S,Shen L,Dangel AW,Hughes JH,Redman KL,Wu LC,Yu CY