Marchf6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Marchf6
Entrez Gene ID	294862
Full Name	membrane associated ring-CH-type finger 6
Synonyms	RGD1310927,RGD1565757
General protein information	Preferred Names membrane associated ring-CH-type finger 6 Names E3 ubiquitin-protein ligase MARCH6 membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q22

mRNA and Protein(s)

mRNA Protein Name

XM_008760826.2 XP_008759048.1 E3 ubiquitin-protein ligase MARCH6 isoform X1

NM_001305274.1 NP_001292203.1 E3 ubiquitin-protein ligase MARCH6
Pathways from BioSystems

mRNA	Protein	Name
XM_008760826.2	XP_008759048.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
NM_001305274.1	NP_001292203.1	E3 ubiquitin-protein ligase MARCH6

Homologies

Pan troglodytes (chimpanzee)	MARCH6	XP_003310676.1
Canis lupus familiaris (dog)	MARCH6	XP_005639724.1
Gallus gallus (chicken)	MARCH6	XP_419012.3
Arabidopsis thaliana (thale cress)	AT4G34100	NP_195136.3
Mus musculus (house mouse)	March6	NP_766194.2
Drosophila melanogaster (fruit fly)	CG1317	NP_647715.2
Xenopus tropicalis (tropical clawed frog)	march6	XP_002938364.2
Homo sapiens (human)	MARCH6	NP_005876.2
Bos taurus (cattle)	MARCH6	NP_001192741.1
Rattus norvegicus (Norway rat)	March6	XP_215517.5
Danio rerio (zebrafish)	march6	XP_005163506.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007204	XP_308581.3
Caenorhabditis elegans (roundworm)	marc-6	NP_492823.2
Oryza sativa (rice)	Os06g0639000	NP_001058157.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Zhang X, Jiang Y, Han W, Liu A, Xie X, Han C, Fan C, Wang H, Zhang H, Ding S, Shan Z, Teng W
Biological trace element research174(2)377-386(2016 Dec)

Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6.
Nakamura N, Harada K, Kato M, Hirose S
Experimental cell research328(1)207-16(2014 Oct)

The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and the cholesterol synthesis pathway.
Zelcer N, Sharpe LJ, Loregger A, Kristiana I, Cook EC, Phan L, Stevenson J, Brown AJ
Molecular and cellular biology34(7)1262-70(2014 Apr)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The E3 ubiquitin ligase TEB4 mediates degradation of type 2 iodothyronine deiodinase.
Zavacki AM, Arrojo E Drigo R, Freitas BC, Chung M, Harney JW, Egri P, Wittmann G, Fekete C, Gereben B, Bianco AC
Molecular and cellular biology29(19)5339-47(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High iodine intake had no discernible effects on USP20. Temporary increases in USP33 and USP19 mRNA levels were observed. The enzymes related to D2 ubiquitination change with prolonged high iodine intake. Increased D2 ubiquitination suppresses the activity of D2, causing a decrease in negative feedback of the hypothalamic-pituitary-thyroid axis.
Title: Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.

TEB4 interacts with and mediates loss of type 2 iodothyronine deiodinase (D2)activity, indicating that D2 ubiquitination and degradation can be tissue specific, depending on WSB-1 and TEB4 expression levels.
Title: The E3 ubiquitin ligase TEB4 mediates degradation of type 2 iodothyronine deiodinase.

The following Marchf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Marchf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa15137	NM_001305274.1 Latest version!	Rattus norvegicus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa45247	XM_008760826.2 Latest version!	Rattus norvegicus membrane associated ring-CH-type finger 6 (March6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Marchf6 ( NM_001305274.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292203.1 Rattus norvegicus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.

Price & Availability↑

CloneID	ORa15137
Clone ID Related Accession (Same CDS sequence)	NM_001305274.1
Accession Version	NM_001305274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07009034.1. On or before Mar 7, 2015 this sequence version replaced XM_215517.8, XM_001065952.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTACC ATCCTTGTGT ATGTACTGGC AGTATTAAGT TTATTCATCA AGAATGCCTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATTTTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCGATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGTTG TTTTGTGGTG ACATGCACAC TTTGTGCATT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCATGGG GGAGCACCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGAGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAG 
AACCCTGATG CCCAGGATGG TCAAGCAGAA GAGGAGGAGG AGGACAATGA GGAGGAAGAT 
GATGCAGGTG TAGAAGATGC TGCTGATGCC AACAATGGGG CCCAAGACGA CATGAACTGG 
AATGCTTTGG AGTGGGACCG GGCTGCTGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTTCT GGAACATGTG TTCTGGGTGG TATCGTTAAA CACACTGTTC 
ATTCTTGTTT TTGCATTTTG TCCCTACCAC ATTGGTCACT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTTCAAGC ATCTCATTTT GAAGGTCTAA TCACGACAAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCCTTGG CCACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTCT GTTAGTTGTG 
GTAGAAATTG GAGTGTTCCC TCTCATCTGT GGCTGGTGGC TGGATATTTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCCCC AGGGACCACA 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTC TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCTATTT ATAGACATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTACTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GCCTCTTGCC TCATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTGCTGCAGG TTGTCTTGCC AGCCCTGCTG 
GAGCAGGGGC ACACCAGGCA GTGGCTGAAG GGGCTTGTTC GTGCATGGAC TGTTACTGCT 
GGATACCTGC TGGACCTTCA TTCCTACTTA CTTGGAGACC AGGAGGAAAG TGAGAACAGT 
GCAAACCAGC AGGTCAACAA CAATCAGCCT GCGCGGAACA ATAACGCCGT CCCTGCTGGG 
GAAGGTCTGC ATGCAGCTCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTAC TAATTGCCAG CCTCATCTGC CTTACCTTAC CAGTATTTGC TGGCCGTTGG 
TTAATGTCAT TTTGGACGGG GACTGCCAAA ATCCATGAGC TGTACACAGC TGCTTGTGGT 
CTCTATGTGT GTTGGCTAAC CATAAGGGCT GTGACGGTGC TGGTGGCCTG GATGCCCCAG 
GGGCGGAGAG TGATTTTCCA GAAGGTCAAA GAGTGGTCCC TCATGATAAT GAAGACTTTG 
ATAGTTGCTG TGCTGCTGGC TGGGGTTGTC CCACTGCTCC TGGGGCTCCT GTTTGAGCTG 
GTTATTGTTG CTCCTCTGAG GGTCCCTCTT GATCAGACTC CCCTTTTCTA CCCTTGGCAG 
GACTGGGCGC TGGGAGTTCT CCATGCCAAA ATCATCGCAG CTATAACACT TATGGGTCCT 
CAGTGGTGGT TGAAGACTGT CATTGAACAG GTTTACGCAA ATGGCATCCG TAACATCGAC 
CTTCACTATA TCATTCGTAA GCTGGCAGCT CCAGTGATCT CTGTGCTCTT GCTCTCCCTG 
TGTATACCGT ATGTCATAGC TTCTGGCGTG GTTCCTTTAT TAGGTGTTAC TGCAGAAATG 
CAGAACTTAG TTCACCGGCG GATTTATCCA TTTTTATTGA TGGTCGTTGT GCTGATGGGA 
ATCCTGTCCT TCCAGGTCCG CCAGTTTAAG CGCCTTTATG AACACATTAA AAATGACAAG 
TACCTTGTGG GGCAGCGCCT GGTGAACTAT GAGCGCAAAT CTGGCAAGCA AGGCCCATCT 
ACGCCACCTC CGGTGTCGTC CCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292203.1
CDS	207..2936
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVLGENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEESENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIIRKLAAPVISVLLLSLCIPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE

Target ORF information:

RefSeq Version	NM_001305274.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated ring-CH-type finger 6 (Marchf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305274.1

ORF Insert Sequence:

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTACC ATCCTTGTGT ATGTACTGGC AGTATTAAGT TTATTCATCA AGAATGCCTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATTTTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCGATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGTTG TTTTGTGGTG ACATGCACAC TTTGTGCATT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCATGGG GGAGCACCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGAGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAG 
AACCCTGATG CCCAGGATGG TCAAGCAGAA GAGGAGGAGG AGGACAATGA GGAGGAAGAT 
GATGCAGGTG TAGAAGATGC TGCTGATGCC AACAATGGGG CCCAAGACGA CATGAACTGG 
AATGCTTTGG AGTGGGACCG GGCTGCTGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTTCT GGAACATGTG TTCTGGGTGG TATCGTTAAA CACACTGTTC 
ATTCTTGTTT TTGCATTTTG TCCCTACCAC ATTGGTCACT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTTCAAGC ATCTCATTTT GAAGGTCTAA TCACGACAAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCCTTGG CCACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTCT GTTAGTTGTG 
GTAGAAATTG GAGTGTTCCC TCTCATCTGT GGCTGGTGGC TGGATATTTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCCCC AGGGACCACA 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTC TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCTATTT ATAGACATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTACTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GCCTCTTGCC TCATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTGCTGCAGG TTGTCTTGCC AGCCCTGCTG 
GAGCAGGGGC ACACCAGGCA GTGGCTGAAG GGGCTTGTTC GTGCATGGAC TGTTACTGCT 
GGATACCTGC TGGACCTTCA TTCCTACTTA CTTGGAGACC AGGAGGAAAG TGAGAACAGT 
GCAAACCAGC AGGTCAACAA CAATCAGCCT GCGCGGAACA ATAACGCCGT CCCTGCTGGG 
GAAGGTCTGC ATGCAGCTCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTAC TAATTGCCAG CCTCATCTGC CTTACCTTAC CAGTATTTGC TGGCCGTTGG 
TTAATGTCAT TTTGGACGGG GACTGCCAAA ATCCATGAGC TGTACACAGC TGCTTGTGGT 
CTCTATGTGT GTTGGCTAAC CATAAGGGCT GTGACGGTGC TGGTGGCCTG GATGCCCCAG 
GGGCGGAGAG TGATTTTCCA GAAGGTCAAA GAGTGGTCCC TCATGATAAT GAAGACTTTG 
ATAGTTGCTG TGCTGCTGGC TGGGGTTGTC CCACTGCTCC TGGGGCTCCT GTTTGAGCTG 
GTTATTGTTG CTCCTCTGAG GGTCCCTCTT GATCAGACTC CCCTTTTCTA CCCTTGGCAG 
GACTGGGCGC TGGGAGTTCT CCATGCCAAA ATCATCGCAG CTATAACACT TATGGGTCCT 
CAGTGGTGGT TGAAGACTGT CATTGAACAG GTTTACGCAA ATGGCATCCG TAACATCGAC 
CTTCACTATA TCATTCGTAA GCTGGCAGCT CCAGTGATCT CTGTGCTCTT GCTCTCCCTG 
TGTATACCGT ATGTCATAGC TTCTGGCGTG GTTCCTTTAT TAGGTGTTAC TGCAGAAATG 
CAGAACTTAG TTCACCGGCG GATTTATCCA TTTTTATTGA TGGTCGTTGT GCTGATGGGA 
ATCCTGTCCT TCCAGGTCCG CCAGTTTAAG CGCCTTTATG AACACATTAA AAATGACAAG 
TACCTTGTGG GGCAGCGCCT GGTGAACTAT GAGCGCAAAT CTGGCAAGCA AGGCCCATCT 
ACGCCACCTC CGGTGTCGTC CCAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Marchf6 ( XM_008760826.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759048.1 Rattus norvegicus membrane associated ring-CH-type finger 6 (March6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45247
Clone ID Related Accession (Same CDS sequence)	XM_008760826.2
Accession Version	XM_008760826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTACC ATCCTTGTGT ATGTACTGGC AGTATTAAGT TTATTCATCA AGAATGCCTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATTTTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCGATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGTTG TTTTGTGGTG ACATGCACAC TTTGTGCATT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCATGGG GGAGCACCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGAGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAG 
AACCCTGATG CCCAGGATGG TCAAGCAGAA GAGGAGGAGG AGGACAATGA GGAGGAAGAT 
GATGCAGGTG TAGAAGATGC TGCTGATGCC AACAATGGGG CCCAAGACGA CATGAACTGG 
AATGCTTTGG AGTGGGACCG GGCTGCTGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTTCT GGAACATGTG TTCTGGGTGG TATCGTTAAA CACACTGTTC 
ATTCTTGTTT TTGCATTTTG TCCCTACCAC ATTGGTCACT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTTCAAGC ATCTCATTTT GAAGGTCTAA TCACGACAAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCCTTGG CCACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTCT GTTAGTTGTG 
GTAGAAATTG GAGTGTTCCC TCTCATCTGT GGCTGGTGGC TGGATATTTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCCCC AGGGACCACA 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTC TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCTATTT ATAGACATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTACTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GCCTCTTGCC TCATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTGCTGCAGG TTGTCTTGCC AGCCCTGCTG 
GAGCAGGGGC ACACCAGGCA GTGGCTGAAG GGGCTTGTTC GTGCATGGAC TGTTACTGCT 
GGATACCTGC TGGACCTTCA TTCCTACTTA CTTGGAGACC AGGAGGAAAG TGAGAACAGT 
GCAAACCAGC AGGTCAACAA CAATCAGCCT GCGCGGAACA ATAACGCCGT CCCTGCTGGG 
GAAGGTCTGC ATGCAGCTCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTAC TAATTGCCAG CCTCATCTGC CTTACCTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGGCG GAGAGTGATT TTCCAGAAGG TCAAAGAGTG GTCCCTCATG 
ATAATGAAGA CTTTGATAGT TGCTGTGCTG CTGGCTGGGG TTGTCCCACT GCTCCTGGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTTCTCCATG CCAAAATCAT CGCAGCTATA 
ACACTTATGG GTCCTCAGTG GTGGTTGAAG ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATCCGTAACA TCGACCTTCA CTATATCATT CGTAAGCTGG CAGCTCCAGT GATCTCTGTG 
CTCTTGCTCT CCCTGTGTAT ACCGTATGTC ATAGCTTCTG GCGTGGTTCC TTTATTAGGT 
GTTACTGCAG AAATGCAGAA CTTAGTTCAC CGGCGGATTT ATCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCTGGC 
AAGCAAGGCC CATCTACGCC ACCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759048.1
CDS	230..2974
Translation	MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVLGENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPHFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEESENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPAFLFSVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIIRKLAAPVISVLLLSLCIPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE

Target ORF information:

RefSeq Version	XM_008760826.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated ring-CH-type finger 6 (March6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760826.2

ORF Insert Sequence:

ATGGACACCG CCGAGGAAGA TATATGTAGA GTGTGTCGGT CAGAAGGAAC ACCTGAGAAA 
CCTCTTTACC ATCCTTGTGT ATGTACTGGC AGTATTAAGT TTATTCATCA AGAATGCCTA 
GTTCAGTGGT TGAAACATAG TCGGAAAGAA TACTGTGAAT TATGCAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCTCC AGATATGCCT TCACGGCTAC CAATCCAAGA CATTTTTGCT 
GGACTGGTTA CAAGTATTGG CACTGCGATA CGATACTGGT TTCATTACAC ACTTGTGGCC 
TTTGCATGGC TGGGAGTTGT TCCTCTTACA GCATGCCGCA TCTACAAGTG CTTGTTTACT 
GGCTCCGTGA GCTCACTACT GACACTGCCA CTAGACATGC TGTCAACGGA AAATTTGTTG 
GCGGATTGCT TGCAGGGTTG TTTTGTGGTG ACATGCACAC TTTGTGCATT CATCAGCCTG 
GTGTGGTTAC GAGAGCAGAT AGTCCATGGG GGAGCACCAA TTTGGCTGGA GCATGCTGCT 
CCACCCTTCA ATGCTGCTGG ACACCACCAA AATGAGGCTC CTGTAGGAGG AAATGGAGCA 
GAAAACCCTG CTGCTGATCA GCCAGCTAAC CCAGCAGGTG AGAATGCAGT TCTGGGTGAG 
AACCCTGATG CCCAGGATGG TCAAGCAGAA GAGGAGGAGG AGGACAATGA GGAGGAAGAT 
GATGCAGGTG TAGAAGATGC TGCTGATGCC AACAATGGGG CCCAAGACGA CATGAACTGG 
AATGCTTTGG AGTGGGACCG GGCTGCTGAA GAGCTTACTT GGGAAAGAAT GCTTGGACTT 
GATGGATCAC TAGTTTTTCT GGAACATGTG TTCTGGGTGG TATCGTTAAA CACACTGTTC 
ATTCTTGTTT TTGCATTTTG TCCCTACCAC ATTGGTCACT TCTCCCTTGT TGGCTTGGGG 
TTTGAGGAGC ATGTTCAAGC ATCTCATTTT GAAGGTCTAA TCACGACAAT TGTTGGATAT 
ATACTTTTAG CAATAACACT GATAATTTGT CATGCCTTGG CCACTCTTGT TAAATTTCAT 
AGGTCTCGTC GCTTGCTGGG CGTCTGCTAT ATTGTGGTTA AGGTCTCTCT GTTAGTTGTG 
GTAGAAATTG GAGTGTTCCC TCTCATCTGT GGCTGGTGGC TGGATATTTG TTCTCTGGAA 
ATGTTTGATG CTACTCTGAA AGATCGAGAG CTGAGCTTTC AGTCAGCCCC AGGGACCACA 
ATGTTTCTGC ACTGGTTGGT GGGAATGGTC TATGTGTTCT ACTTTGCCTC CTTCATCCTG 
TTACTGAGAG AGGTACTTCG ACCTGGTGTC TTATGGTTTC TGAGGAATTT GAATGATCCA 
GATTTTAATC CAGTACAGGA AATGATTCAC TTGCCTATTT ATAGACATCT TCGAAGATTT 
ATTTTGTCAG TGATTGTCTT TGGCTCCATT GTACTTCTGA TGCTCTGGCT TCCTATACGT 
ATAATTAAGA GCCTCTTGCC TCATTTTCTT CCTTACAATG TCATGCTCTA CAGTGATGCT 
CCGGTGAGTG AGCTGTCCCT TGAGCTGCTC CTGCTGCAGG TTGTCTTGCC AGCCCTGCTG 
GAGCAGGGGC ACACCAGGCA GTGGCTGAAG GGGCTTGTTC GTGCATGGAC TGTTACTGCT 
GGATACCTGC TGGACCTTCA TTCCTACTTA CTTGGAGACC AGGAGGAAAG TGAGAACAGT 
GCAAACCAGC AGGTCAACAA CAATCAGCCT GCGCGGAACA ATAACGCCGT CCCTGCTGGG 
GAAGGTCTGC ATGCAGCTCA CCAAGCCATA CTCCAGCAGG GAGGACCTGT TGGCTTTCAG 
CCTTACCGCC GGCCTTTAAA TTTCCCACTC AGGATATTTC TGCTGATTGT CTTCATGTGC 
ATAACATTAC TAATTGCCAG CCTCATCTGC CTTACCTTAC CAGCCTTTCT ATTTTCAGTA 
TTTGCTGGCC GTTGGTTAAT GTCATTTTGG ACGGGGACTG CCAAAATCCA TGAGCTGTAC 
ACAGCTGCTT GTGGTCTCTA TGTGTGTTGG CTAACCATAA GGGCTGTGAC GGTGCTGGTG 
GCCTGGATGC CCCAGGGGCG GAGAGTGATT TTCCAGAAGG TCAAAGAGTG GTCCCTCATG 
ATAATGAAGA CTTTGATAGT TGCTGTGCTG CTGGCTGGGG TTGTCCCACT GCTCCTGGGG 
CTCCTGTTTG AGCTGGTTAT TGTTGCTCCT CTGAGGGTCC CTCTTGATCA GACTCCCCTT 
TTCTACCCTT GGCAGGACTG GGCGCTGGGA GTTCTCCATG CCAAAATCAT CGCAGCTATA 
ACACTTATGG GTCCTCAGTG GTGGTTGAAG ACTGTCATTG AACAGGTTTA CGCAAATGGC 
ATCCGTAACA TCGACCTTCA CTATATCATT CGTAAGCTGG CAGCTCCAGT GATCTCTGTG 
CTCTTGCTCT CCCTGTGTAT ACCGTATGTC ATAGCTTCTG GCGTGGTTCC TTTATTAGGT 
GTTACTGCAG AAATGCAGAA CTTAGTTCAC CGGCGGATTT ATCCATTTTT ATTGATGGTC 
GTTGTGCTGA TGGGAATCCT GTCCTTCCAG GTCCGCCAGT TTAAGCGCCT TTATGAACAC 
ATTAAAAATG ACAAGTACCT TGTGGGGCAG CGCCTGGTGA ACTATGAGCG CAAATCTGGC 
AAGCAAGGCC CATCTACGCC ACCTCCGGTG TCGTCCCAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Biological trace element research174(2)377-386(2016 Dec)
Zhang X,Jiang Y,Han W,Liu A,Xie X,Han C,Fan C,Wang H,Zhang H,Ding S,Shan Z,Teng W

Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6.
Experimental cell research328(1)207-16(2014 Oct)
Nakamura N,Harada K,Kato M,Hirose S

The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and the cholesterol synthesis pathway.
Molecular and cellular biology34(7)1262-70(2014 Apr)
Zelcer N,Sharpe LJ,Loregger A,Kristiana I,Cook EC,Phan L,Stevenson J,Brown AJ

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The E3 ubiquitin ligase TEB4 mediates degradation of type 2 iodothyronine deiodinase.
Molecular and cellular biology29(19)5339-47(2009 Oct)
Zavacki AM,Arrojo E Drigo R,Freitas BC,Chung M,Harney JW,Egri P,Wittmann G,Fekete C,Gereben B,Bianco AC

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Marchf6 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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