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Tmem259 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Tmem259
Entrez Gene ID 299608
Full Name transmembrane protein 259
Synonyms Mbrl,RGD1311136
General protein information
Preferred Names

transmembrane protein 259

Names

membralin
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

7

7q11

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001100886.1 NP_001094356.1 membralin
XM_006240869.3 XP_006240931.1 membralin isoform X1
XM_006240870.3 XP_006240932.1 membralin isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) TMEM259 NP_001028198.1
Pan troglodytes (chimpanzee) C19H19orf6 XP_003954544.1
Xenopus tropicalis (tropical clawed frog) tmem259 NP_001072254.1
Bos taurus (cattle) TMEM259 XP_002689215.2
Danio rerio (zebrafish) tmem259 XP_700127.3
Canis lupus familiaris (dog) C20H19orf6 XP_533963.2
Gallus gallus (chicken) C19ORF6 XP_418222.2
Mus musculus (house mouse) Tmem259 NP_001003949.2
Rattus norvegicus (Norway rat) Mbrl NP_001094356.1

Gene Ontology


Bibliography

Related articles in PubMed

The critical role of membralin in postnatal motor neuron survival and disease.
Yang B, Qu M, Wang R, Chatterton JE, Liu XB, Zhu B, Narisawa S, Millan JL, Nakanishi N, Swoboda K, Lipton SA, Zhang D
eLife4(2015 May)

Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.
Andersson O, von Euler G
Brain research. Gene expression patterns1(3-4)205-12(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

genomic organization and expression (membralin)
Title: Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.

The following Tmem259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa13784 NM_001100886.1
Latest version!		 Rattus norvegicus transmembrane protein 259 (Tmem259), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
ORa29827 XM_006240869.3
Latest version!		 Rattus norvegicus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
ORa29828 XM_006240870.3
Latest version!		 Rattus norvegicus transmembrane protein 259 (Tmem259), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa13784
Clone ID Related Accession (Same CDS sequence) NM_001100886.1
Accession Version NM_001100886.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-27
Organism Rattus norvegicus(Norway rat)
Product membralin
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK843921.1 and BC100259.1. On or before Nov 23, 2008 this sequence version replaced XM_216835.4, XM_001076923.1. ##Evidence-Data-START## Transcript exon combination :: BC100259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAGGCCAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCCGACCAG 
TACGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGAC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA CGCCTACCAC TACCGCTTTA ACGGCCAGTA CAGCAGCTTG 
GCCCTGGTCA CGTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGTTGCA GACACCCCCG 
CTGGGCCCTG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAATAACTC TGGCTCCCCT 
GCCACCCCAG ATCCCAGTCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGAGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCTGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCGGACGCGT CCTTCCTGTC GGGGCTGAGC 
GCCTCACTGC TGGAGCGGCG GCCAGCAGCC CCCAGTGCCC CGGACAGCGC ACGACCTGAC 
CCTGGGGCCT CTCTGGAGGA CGCACCTGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001094356.1
CDS29..1753
Translation

Target ORF information:

RefSeq Version NM_001100886.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus transmembrane protein 259 (Tmem259), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001100886.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAGGCCAT CATGTCTGAG 
TTCTTCAACG ACACCACCAC GGCCTTCTAC ATCATCCTCA TCGTGTGGCT GGCCGACCAG 
TACGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGAC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA CGCCTACCAC TACCGCTTTA ACGGCCAGTA CAGCAGCTTG 
GCCCTGGTCA CGTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGTTGCA GACACCCCCG 
CTGGGCCCTG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAATAACTC TGGCTCCCCT 
GCCACCCCAG ATCCCAGTCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGAGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCTGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCGGACGCGT CCTTCCTGTC GGGGCTGAGC 
GCCTCACTGC TGGAGCGGCG GCCAGCAGCC CCCAGTGCCC CGGACAGCGC ACGACCTGAC 
CCTGGGGCCT CTCTGGAGGA CGCACCTGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa29827
Clone ID Related Accession (Same CDS sequence) XM_006240869.3
Accession Version XM_006240869.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1674bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product membralin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGCGCCCTT GCTGACCGTC ATCCTGGCTC TCGTCGGGAT GGAGGCCATC 
ATGTCTGAGT TCTTCAACGA CACCACCACG GCCTTCTACA TCATCCTCAT CGTGTGGCTG 
GCCGACCAGT ACGATGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGACA CTGGCTGAGG 
TTCTTCTACC TCTACCACTT CGCCTTCTAC GCCTACCACT ACCGCTTTAA CGGCCAGTAC 
AGCAGCTTGG CCCTGGTCAC GTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTATG AGTTGCCCGC CATCCTGCAG CAGATCCGAA TCCAGGAGAT GCTGTTGCAG 
ACACCCCCGC TGGGCCCTGG GACCCCCACG GCGCTGCCTG ACGACCTCAA CAATAACTCT 
GGCTCCCCTG CCACCCCAGA TCCCAGTCCT CCCCTCGCGC TGGGCCCCAG CTCCAGCCCC 
GCGCCCACTG GCGGAGCATC TGGGCCTGGC TCACTGGGCG CTGGGGCCTC AGTATCTGGC 
AGTGACCTAG GTTGGGTGGC CGAGACCGCC GCCATCATCT CGGACGCGTC CTTCCTGTCG 
GGGCTGAGCG CCTCACTGCT GGAGCGGCGG CCAGCAGCCC CCAGTGCCCC GGACAGCGCA 
CGACCTGACC CTGGGGCCTC TCTGGAGGAC GCACCTGCCC CTGCCGGGTC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006240931.1
CDS24..1697
Translation

Target ORF information:

RefSeq Version XM_006240869.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006240869.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGCGCCCTT GCTGACCGTC ATCCTGGCTC TCGTCGGGAT GGAGGCCATC 
ATGTCTGAGT TCTTCAACGA CACCACCACG GCCTTCTACA TCATCCTCAT CGTGTGGCTG 
GCCGACCAGT ACGATGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGACA CTGGCTGAGG 
TTCTTCTACC TCTACCACTT CGCCTTCTAC GCCTACCACT ACCGCTTTAA CGGCCAGTAC 
AGCAGCTTGG CCCTGGTCAC GTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTATG AGTTGCCCGC CATCCTGCAG CAGATCCGAA TCCAGGAGAT GCTGTTGCAG 
ACACCCCCGC TGGGCCCTGG GACCCCCACG GCGCTGCCTG ACGACCTCAA CAATAACTCT 
GGCTCCCCTG CCACCCCAGA TCCCAGTCCT CCCCTCGCGC TGGGCCCCAG CTCCAGCCCC 
GCGCCCACTG GCGGAGCATC TGGGCCTGGC TCACTGGGCG CTGGGGCCTC AGTATCTGGC 
AGTGACCTAG GTTGGGTGGC CGAGACCGCC GCCATCATCT CGGACGCGTC CTTCCTGTCG 
GGGCTGAGCG CCTCACTGCT GGAGCGGCGG CCAGCAGCCC CCAGTGCCCC GGACAGCGCA 
CGACCTGACC CTGGGGCCTC TCTGGAGGAC GCACCTGCCC CTGCCGGGTC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa29828
Clone ID Related Accession (Same CDS sequence) XM_006240870.3
Accession Version XM_006240870.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product membralin isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGGATGGA GGCCATCATG TCTGAGTTCT TCAACGACAC CACCACGGCC 
TTCTACATCA TCCTCATCGT GTGGCTGGCC GACCAGTACG ATGCCATCTG CTGCCACACC 
AACACCAGCA AGCGACACTG GCTGAGGTTC TTCTACCTCT ACCACTTCGC CTTCTACGCC 
TACCACTACC GCTTTAACGG CCAGTACAGC AGCTTGGCCC TGGTCACGTC CTGGCTCTTC 
ATCCAGCATT CCATGATCTA CTTCTTCCAC CACTATGAGT TGCCCGCCAT CCTGCAGCAG 
ATCCGAATCC AGGAGATGCT GTTGCAGACA CCCCCGCTGG GCCCTGGGAC CCCCACGGCG 
CTGCCTGACG ACCTCAACAA TAACTCTGGC TCCCCTGCCA CCCCAGATCC CAGTCCTCCC 
CTCGCGCTGG GCCCCAGCTC CAGCCCCGCG CCCACTGGCG GAGCATCTGG GCCTGGCTCA 
CTGGGCGCTG GGGCCTCAGT ATCTGGCAGT GACCTAGGTT GGGTGGCCGA GACCGCCGCC 
ATCATCTCGG ACGCGTCCTT CCTGTCGGGG CTGAGCGCCT CACTGCTGGA GCGGCGGCCA 
GCAGCCCCCA GTGCCCCGGA CAGCGCACGA CCTGACCCTG GGGCCTCTCT GGAGGACGCA 
CCTGCCCCTG CCGGGTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006240932.1
CDS24..1664
Translation

Target ORF information:

RefSeq Version XM_006240870.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus transmembrane protein 259 (Tmem259), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006240870.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGCGGCGCG 
GCGCCCGTGC GCGGCCCGCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCGCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAGTTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT GGAGCATGTT 
CGAGACCGAT GGCCACGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCCAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTAGACCT GGAGCCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTTTCC GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACCAG GGGGCGCCGA TGCCCTGAAT 
GACAGCCAGG ACTTCCCTTT CCCAGAGACA CCAGCCAAAG CGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCGCTGGA ATATGGCTTC CTGCGACTGT CGCAAGCCAC GCGCCAGCGC 
CTGAGCATCC CTGTCATGGT AGTCACCCTA GACCCCACGC GGGAGCAGTG CTTCGGGGAC 
CGCTTCAGCC GCCTGCTGCT GGACGAGTTC CTGGGCTACG ACGACGTCCT CATGTCTAGT 
GTGAAGGGCC TGGCAGAGAA TGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCACGCACAT CCTACCTGGC AGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGTGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGGATGGA GGCCATCATG TCTGAGTTCT TCAACGACAC CACCACGGCC 
TTCTACATCA TCCTCATCGT GTGGCTGGCC GACCAGTACG ATGCCATCTG CTGCCACACC 
AACACCAGCA AGCGACACTG GCTGAGGTTC TTCTACCTCT ACCACTTCGC CTTCTACGCC 
TACCACTACC GCTTTAACGG CCAGTACAGC AGCTTGGCCC TGGTCACGTC CTGGCTCTTC 
ATCCAGCATT CCATGATCTA CTTCTTCCAC CACTATGAGT TGCCCGCCAT CCTGCAGCAG 
ATCCGAATCC AGGAGATGCT GTTGCAGACA CCCCCGCTGG GCCCTGGGAC CCCCACGGCG 
CTGCCTGACG ACCTCAACAA TAACTCTGGC TCCCCTGCCA CCCCAGATCC CAGTCCTCCC 
CTCGCGCTGG GCCCCAGCTC CAGCCCCGCG CCCACTGGCG GAGCATCTGG GCCTGGCTCA 
CTGGGCGCTG GGGCCTCAGT ATCTGGCAGT GACCTAGGTT GGGTGGCCGA GACCGCCGCC 
ATCATCTCGG ACGCGTCCTT CCTGTCGGGG CTGAGCGCCT CACTGCTGGA GCGGCGGCCA 
GCAGCCCCCA GTGCCCCGGA CAGCGCACGA CCTGACCCTG GGGCCTCTCT GGAGGACGCA 
CCTGCCCCTG CCGGGTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The critical role of membralin in postnatal motor neuron survival and disease.
eLife4(2015 May)
Yang B,Qu M,Wang R,Chatterton JE,Liu XB,Zhu B,Narisawa S,Millan JL,Nakanishi N,Swoboda K,Lipton SA,Zhang D


book

Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.
Brain research. Gene expression patterns1(3-4)205-12(2002 Oct)
Andersson O,von Euler G