Col7a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col7a1
Entrez Gene ID	301012
Full Name	collagen type VII alpha 1 chain
General protein information	Preferred Names collagen type VII alpha 1 chain Names collagen alpha-1(VII) chain collagen, type VII, alpha 1 procollagen, type VII, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106858.1	NP_001100328.1	collagen alpha-1(VII) chain precursor
XM_006243759.3	XP_006243821.1	collagen alpha-1(VII) chain isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col7a1	NP_001100328.1
Bos taurus (cattle)	COL7A1	XP_002697097.2
Mus musculus (house mouse)	Col7a1	NP_031764.2
Gallus gallus (chicken)	COL7A1	XP_425157.4
Xenopus tropicalis (tropical clawed frog)	col7a1	XP_002933125.2
Canis lupus familiaris (dog)	COL7A1	NP_001002980.1
Danio rerio (zebrafish)	col7a1	XP_002662551.3
Homo sapiens (human)	COL7A1	NP_000085.1
Pan troglodytes (chimpanzee)	COL7A1	XP_003950149.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

Rat model for dominant dystrophic epidermolysis bullosa: glycine substitution reduces collagen VII stability and shows gene-dosage effect.
Nystr?m A, Buttgereit J, Bader M, Shmidt T, Ozcelik C, Hausser I, Bruckner-Tuderman L, Kern JS
PloS one8(5)e64243(2013)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Insight into interactions of the von-Willebrand-factor-A-like domain 2 with the FNIII-like domain 9 of collagen VII by NMR and SPR.
Leineweber S, Sch?nig S, Seeger K
FEBS letters585(12)1748-52(2011 Jun)

Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment.
Gebauer JM, Keene DR, Olsen BR, Sorokin LM, Paulsson M, Wagener R
Matrix biology : journal of the International Society for Matrix Biology28(8)456-62(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A spontaneous glycine to aspartic acid substitution, p.G1867D, within the main structural domain of collagen VII is associated with dominant dystrophic epidermolysis bullosa.
Title: Rat model for dominant dystrophic epidermolysis bullosa: glycine substitution reduces collagen VII stability and shows gene-dosage effect.

The following Col7a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col7a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05026	NM_001106858.1 Latest version!	Rattus norvegicus collagen type VII alpha 1 chain (Col7a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$2268.00 $3240.00
ORa31567	XM_006243759.3 Latest version!	Rattus norvegicus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col7a1 ( NM_001106858.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100328.1 Rattus norvegicus collagen type VII alpha 1 chain (Col7a1), mRNA.

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CloneID	ORa05026
Clone ID Related Accession (Same CDS sequence)	NM_001106858.1
Accession Version	NM_001106858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(VII) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 4, 2007 this sequence version replaced XM_001075419.1, XM_238554.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGCTGC GGCTCCTGGT CGCTGCGCTC TGCACTGCCG AGATCCTGGT GGGGGCGCCC 
GGAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACCC GCCTTTATGC TGCTGACATT 
GTGTTCTTGA TTGACGGCTC CTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGGGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA ATGCACAGGG CGTCCGATTT 
GCAACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGGGTGACA CCATTCGTGC CATCCGGGAG CTCAGCTATA AGGGTGGTAA CACTCGAACG 
GGGGCTGCTC TTCTCCACGT TTCCGACCGG GTCTTCCTGC CTCATCTAAC ACGTCCTGGC 
ATCCCCAAGG TCTGTATCCT GATCACAGAT GGGAAATCCC AGGACTTAGT GGACACAGCT 
GCCCAAAAAC TGAAGAGGCA GGGGGTCAAG CTGTTTGCCG TGGGAATCAA GAACGCCGAT 
CCTGAGGAAC TGAAGCGCAT CGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTTGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCC CTCATTTCCC GGAGGGTGTG CACCACGGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCCGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTGTCTGAGC CAAGTAGCCA ATCCTTACGA GTACAGTGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AAATTCAGTA CACGCCCCTG ACGGGGCTAG GACAGCCACT GCCAAGTGAG 
CGAAAGGAGG TGAACGTCCC GGCTGGTGAG ACCAGCATGC GACTACAGGG TCTCCGGCCA 
CTAACTGACT ACCAAGTGAC TGTGGTGGCC CTCTATGCTA ACAGCATCGG AGAGGCTGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAGC TGAGCGTCCA GAACATCACA 
TCCCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGCGTAACA 
TGGCGGGACC TCAGTGGTGG GGGGGCACAG CAGCAGGACC TGAGCCCTGG GCAGGGGTCA 
GTGTTTTTAG ATCACTTGAA GCCTGGCACG GACTATGAGG TGACTGTGAG CGCCCTATTT 
GGCCGCAGCG TGGGGCCTGC TGCTTCTCTG ACTACCCGCA CTGCCTCTTC TGTTGAGCAG 
ACACTGCGCC CCATCATCCT GAGCCCCACA TCTATTCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCCT GGAGTGGCAT CGTGAGAGTG GCTTGGAACC ACCACAGAAA 
GTGGAACTGC TCCCTGATGT GACCCGCTAC CAGTTGGATG GGTTGCAGCC AGGCACTGAG 
TATCGCCTCA CGCTCTACAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCGACTGTG 
GTTCCTACTG GGCTAGAGCA GCTGGTGGGC CCGGTGATGA ATTTACAAGC CACAGAACTA 
CCTGGGCAGC GGCTGCGAGT GTCTTGGAAC CCAGTCCTCG GTGCCACCGA GTACCGTTTC 
ACTGTACGCA CCCCCCAGGG GGTCGAGCGG ACTTTGTTGC TTCCTGGGAG TCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCCGGGATT AGCTACACAG TGCGAGTGTC TGCTCGAGTG 
GGCACTCATG AGGGTGATGC CAGCACCCTC ACCATCCACA GAGATCAAGA AGCCCCACTT 
ATTATCCCAG GACTGCAGGT TGTGGCATCA GATGCAACAA GAATTAGAGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATT AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGTCAGACGC TGGCCCCAGA CTCTACTGTC ACAGACATCC TGGGGCTGCA GCCTGGAACC 
TCTTATCAAG TGGCTGTGTC AGCCCTTCGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTCGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGTAGC 
TCATCTGTCA GCATTACCTG GACTGGGGTT CCTGGCGCCA CCGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCAA TTGGTTTCTG GGGACGCCAC GGTGGCTGAA 
ATAGATGGAC TGGAGCCAGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA TGTAGCCGGT 
GTGGATGGAG CCCCGGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT AGGCAGTGTG 
TCAAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTCC GCGTCACTTG GGTGGGAGTC 
CCTGGAGCCA CATCCTATAG ACTGGCTTGG GGACGTAGTG AAGGTGGACC TATGAGACAC 
CAGATAGTCC CAGGAAACAA AGACTCTGCA GAGATAACAG GTCTGGAAGG CGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCCGT GTCCATTGTC 
GTCACCACAC CGCCTGAGGT CCCAGCACCT CTGGAGACCC TTCAGGTAGT GCAGAGTGGG 
GAGCACTCCT TGAGGCTGCA CTGGAACCGA GTCCCCGGAG CACAGGGCTT CCGTCTGCAT 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTCG GACTGGAGCC AGCAACGAAG TACCATGTAT GGCTGAGTGT CCTGGGCCAG 
CCTGGAGAAG CTGCCCCTAG GAAGGTGACT GCTTACACTG AGACTCCTCA CATCCCGAGC 
ACTGGGCTAC GGGTGGTGGA CACCTCTGTC AACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGCTATAT CTTGTCCTGG CGGCCACTCA GAGGAACTGG CCAGGAAGTG 
CCGGGGGGCC CGCAGACACT GCCAGGGAAC TCAAGCTCCC ACAGAGTGAC AGGCCTGGAA 
CCTGGGGTTC CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCACTGTCA CACAAAAGCC AGCGTGTCCC CATGGCCAGG TGGACGTGGT GATCCTGTTA 
CATGCCACTC GAGACAACGC TCACAATGCA GAAGCTGTAA AGAGGTTCCT GGAAAGGCTG 
GTGTCTGCAC TCGGGCCTCT TGGTCCACAG GCTGCTCAGG TTGGCCTGCT GTCCTACAGC 
CACCGGCCCT CCCCACTGTT CCCGCTGAAT AGTTCTCACG ATCTTGGTGT CATCTTGCAA 
AAGATCCGTG ACATCCCCTA TGTGGACCCA AGTGGTAACA ACCTAGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCACCCAAT GCTCCCGGTC GCCGTCGGCA AGTGCCAGGC 
GTGATGGTAC TGTTAGTGGA TGAACCTCTG AGAGAAGACA TATTGAGCCC CATTCGTGAG 
GCCCAGGCTT CTGGGCTCAA GGTGATGATG CTGGGTCTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGCCTCT TGGCACCAGG CATGGACCCC ATCCAGACCT TCTTTGCTGT GGATAATGGC 
TTAGACTTGG ACCGAGCAGG CAGTGATCTG GCTGTAGCCC TATGTCAGGC AGCTGTGGCC 
ATACAGCCAC AACTAGAGCC TTGTGCTGTG CCCTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTGACGG GACCGCAAGG ACAGGCTGGA CCTCCTGGTC CCCCCGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCACA AGGTGCTCCC GGAAGTACCC AAGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGCCC TCCAGGCAGT CCTGGCCTAC CTGGTGTTCC TGGGTCTCCT 
GGCGTTAAGG GCTCCCCAGG GTGGTCTGGC CCTCGTGGGG ATCGGGGAGA ACGAGGGCCT 
CAAGGCCCAA AAGGGGAGCC GGGGGAGCCT GGGCAAGTCA TTGGAGGCGG AAGGCCAGGG 
CTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGACCCC CTGGACCTCA TGGCCCTTTG 
GGGGACCCAG GACCTCGTGG CCCCCCTGGG CTTCCTGGAA CATCAGTGAA GGGTGACAAA 
GGTGACCGTG GGGAACGGGG TCCCCCAGGA CCAGGCACTG GTGCCAGTGA GCAAGGCAGC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCACCTGG TCGAACTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGCCCAG GCCTTCCAGG ACAGCCTGGG 
GTTCCAGGGG AACCGGGCCT GCGGGGAGCT CCTGGAGTTA CTGGGCCCAA AGGTGATCGG 
GGACTGACAG GGACCCCGGG TGAGCCTGGA GAGAAGGGTG AACGGGGACC ACCTGGTCCC 
GTGGGACCCC AGGGGCTTCC CGGAGCTGCT GGACGTCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCCCTGGCCG CCGGGGAGAA AAGGGAGAGC CTGGTCGCCC TGGGGATCCT 
GCATTGGGCC CTGGTGGTGC TGGAGCCAAG GGAGAAAAGG GAGATGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAT CAAAGGAGAG CAGGGTGCAC CTGGGTTGGC TCTTCCTGGA 
GACCCTGGCC CCAAAGGAGA CCCTGGAGAC AGAGGTCCCA TTGGCCTCAC TGGCAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAGGGAG AACCTGGGCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCTCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGT 
GCCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GCGGCCTCCG GGGGGCACCT 
GGGCCCCGGG GGCCTGTGGG TGAGAAGGGG AATGAGGGGG ATCCTGGAGA AGATGGCCGG 
AATGGCACCC CTGGACCATC TGGACCCAAG GGTGACCGTG GGGAGCCGGG CCCCCCGGGT 
CTCCCTGGAC GGCTGGTGGA TGCCGCCCTT GAATCCAGAG ACAAGGGAGA GCCTGGACAG 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGCCCCCCTG GAGCCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CAGGTGTTCG AGGTCCTGCA 
GGAGACAAGG GTGATCGGGG ATCCCCAGGG CTGGATGGCC GAAATGGGCT AGATGGGAAA 
CCAGGAGCCC CTGGCCCTCC AGGGCTACAC GGTGCTTCAG GGAAAGCTGG GGACCCTGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCAC CTGGTCCCCC TGGCCCTCCT 
GGAGTACCGG GAAAGCCAGG CGATGACGGC AAACCCGGCC TGAATGGGAA AAACGGAGAA 
CCTGGGGATC CCGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGTCCCTGGA 
AGGGAAGGTC CTGACGGTCC CAAGGGTGAA CGCGGAGCTC CTGGTAATCC CGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAGTTACAG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCG GGGAGCTTGC CGAATGCGGA GCGGTTCCTG 
GAAACTGCTG GCATCAAGGT GTCAGCCCTG CGGGAGATTG TGGACACCTG GGGTGAGAGT 
TCTGGCAGCT TCCTGCTGGT GCCTGAGCGG AGACAAGGTC CCAAGGGGGA CCCTGGTGAC 
CCAGGCCCTC CAGGCAAGGA GGGTTCCATT GGTCTTCCTG GAGAACGTGG GCTAAAGGGA 
GAACGAGGAG ACCCTGGCCC TCAGGGGCCT CCTGGTCTCG CCCTTGGTGA GAGGGGCCCA 
CCTGGTCCAT CTGGCCTTGC CGGGGAACCA GGAAAGCCTG GAATTCCTGG GCTCCCAGGT 
CGGGCTGGGG CTGCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGAACAGGG CAGAGATGGC CATCCCGGCC TTCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCTA GGTCCTGGAC CCGCTAGAGA ACAAGGACCT 
CCTGGACTCA AGGGTGCTAA GGGGGAGCCA GGCAGTGATG GTGTCCCAGG ACCCAAAGGA 
GACAGGGGTG TACCCGGCAT CAAAGGAGAT GCAGGAGAGC CAGGAAAGAG GGGTCCTGAT 
GGTAACCCAG GTCTGCCAGG AGAGCGCGGT GTGTCTGGAC CTGAAGGGAA GCCGGGTCTG 
CAGGGCCCGA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCAGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCAGGGCCC CAGGGACCTT CTGGCCTGAA GGGGGAGCCC 
GGAGAGACAG GACCCCCAGG ACGGGGTCTG CCTGGACCTA CTGGAGCTGT GGGACTTCCT 
GGCCCTCCTG GCCCTTCAGG CCTCGTGGGC CCACAAGGCT CACCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCCCCA GGCCGGGATG GTAGCAGCGG AAAGGACGGA 
GAAAGAGGAG GCCCTGGCGT GCCAGGGTTA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GACCTGTGGG GGCCCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCAGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAG 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCA TGCAGGAGAG 
CCTGGCGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTCAAGGGC 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGTGCT CCTGGTATTG TTGGATTTCC TGGTCAGCCA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGCGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCGGG GTCAGTGGGA 
GCACCTGGCG CCTCTGGATT CAAAGGAGAC AAGGGAGACT CAGGAGCAGG GCTGCCAGGG 
CCCCGAGGCG AGCGTGGTGA ACCAGGTCTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTCATGGG GCCCCCTGGC AGCCGGGGAG AGCGAGGAGA GAAGGGCGAC 
CCTGGTGCTG CAGGACTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTGCA 
GGGCCACGAG GTGCCAAGGG GGATATGGGT GAACGAGGGC CTCGAGGCAT AGATGGTGAC 
CAAGGACCTC GGGGTGAAAG TGGGGATCCT GGGGACAAGG GCTCCAAAGG AGAGCCTGGA 
GACAAAGGCT CGGCTGGCTC CACTGGAGTT CGCGGACTCA CAGGACCCAA GGCAAGGCGA 
GCCAGGTGCT GCAGGGATCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100328.1
CDS	1..7767
Translation	MRLRLLVAALCTAEILVGAPGVWAQPRDRVTCTRLYAADIVFLIDGSSSIGRSNFREVRGFLEGLVLPFSGAANAQGVRFATVQYSDDPQTEFGLDTLGSGGDTIRAIRELSYKGGNTRTGAALLHVSDRVFLPHLTRPGIPKVCILITDGKSQDLVDTAAQKLKRQGVKLFAVGIKNADPEELKRIASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKIQYTPLTGLGQPLPSERKEVNVPAGETSMRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSVQNITSHSLLVAWRRVPGANGYRVTWRDLSGGGAQQQDLSPGQGSVFLDHLKPGTDYEVTVSALFGRSVGPAASLTTRTASSVEQTLRPIILSPTSILLSWNLVPEARGYRLEWHRESGLEPPQKVELLPDVTRYQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVGPVMNLQATELPGQRLRVSWNPVLGATEYRFTVRTPQGVERTLLLPGSQTTFDLDDVRAGISYTVRVSARVGTHEGDASTLTIHRDQEAPLIIPGLQVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSQTLAPDSTVTDILGLQPGTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSQLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYRLAWGRSEGGPMRHQIVPGNKDSAEITGLEGGVSYSVRVTALVGDREGAPVSIVVTTPPEVPAPLETLQVVQSGEHSLRLHWNRVPGAQGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYHVWLSVLGQPGEAAPRKVTAYTETPHIPSTGLRVVDTSVNSVTLTWTPVSGASSYILSWRPLRGTGQEVPGGPQTLPGNSSSHRVTGLEPGVPYVFSLTPIQSGVRGSEITVTQKPACPHGQVDVVILLHATRDNAHNAEAVKRFLERLVSALGPLGPQAAQVGLLSYSHRPSPLFPLNSSHDLGVILQKIRDIPYVDPSGNNLGTAVTTAHRYLLAPNAPGRRRQVPGVMVLLVDEPLREDILSPIREAQASGLKVMMLGLVGADPEQLRLLAPGMDPIQTFFAVDNGLDLDRAGSDLAVALCQAAVAIQPQLEPCAVPCPKGQKGEPGVTGPQGQAGPPGPPGLPGRTGAPGPQGAPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGVKGSPGWSGPRGDRGERGPQGPKGEPGEPGQVIGGGRPGLPGKKGDPGPSGPPGPHGPLGDPGPRGPPGLPGTSVKGDKGDRGERGPPGPGTGASEQGSPGLPGLPGSPGPQGPPGRTGEKGEKGDCEDGGPGLPGQPGVPGEPGLRGAPGVTGPKGDRGLTGTPGEPGEKGERGPPGPVGPQGLPGAAGRPGVEGPEGPPGPPGRRGEKGEPGRPGDPALGPGGAGAKGEKGDAGLPGPRGASGIKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGAPGEPGLPGKAGERGLRGAPGPRGPVGEKGNEGDPGEDGRNGTPGPSGPKGDRGEPGPPGLPGRLVDAALESRDKGEPGQEGPRGPKGDPGPPGASGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGSPGLDGRNGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKPGDDGKPGLNGKNGEPGDPGEDGRKGEKGDSGVPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGVTGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERFLETAGIKVSALREIVDTWGESSGSFLLVPERRQGPKGDPGDPGPPGKEGSIGLPGERGLKGERGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGAAGEAGRPGERGERGEKGERGEQGRDGHPGLPGPPGPPGPKVAIEELGPGPAREQGPPGLKGAKGEPGSDGVPGPKGDRGVPGIKGDAGEPGKRGPDGNPGLPGERGVSGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGERGGPGVPGLPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGHAGEPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGIVGFPGQPGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSVGAPGASGFKGDKGDSGAGLPGPRGERGEPGLRGEDGHPGQEGPRGLMGPPGSRGERGEKGDPGAAGLKGDKGDSAVIEGPAGPRGAKGDMGERGPRGIDGDQGPRGESGDPGDKGSKGEPGDKGSAGSTGVRGLTGPKARRARCCRDPR

Target ORF information:

RefSeq Version	NM_001106858.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type VII alpha 1 chain (Col7a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106858.1

ORF Insert Sequence:

ATGAGGCTGC GGCTCCTGGT CGCTGCGCTC TGCACTGCCG AGATCCTGGT GGGGGCGCCC 
GGAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACCC GCCTTTATGC TGCTGACATT 
GTGTTCTTGA TTGACGGCTC CTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGGGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA ATGCACAGGG CGTCCGATTT 
GCAACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGGGTGACA CCATTCGTGC CATCCGGGAG CTCAGCTATA AGGGTGGTAA CACTCGAACG 
GGGGCTGCTC TTCTCCACGT TTCCGACCGG GTCTTCCTGC CTCATCTAAC ACGTCCTGGC 
ATCCCCAAGG TCTGTATCCT GATCACAGAT GGGAAATCCC AGGACTTAGT GGACACAGCT 
GCCCAAAAAC TGAAGAGGCA GGGGGTCAAG CTGTTTGCCG TGGGAATCAA GAACGCCGAT 
CCTGAGGAAC TGAAGCGCAT CGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTTGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCC CTCATTTCCC GGAGGGTGTG CACCACGGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCCGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTGTCTGAGC CAAGTAGCCA ATCCTTACGA GTACAGTGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AAATTCAGTA CACGCCCCTG ACGGGGCTAG GACAGCCACT GCCAAGTGAG 
CGAAAGGAGG TGAACGTCCC GGCTGGTGAG ACCAGCATGC GACTACAGGG TCTCCGGCCA 
CTAACTGACT ACCAAGTGAC TGTGGTGGCC CTCTATGCTA ACAGCATCGG AGAGGCTGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAGC TGAGCGTCCA GAACATCACA 
TCCCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGCGTAACA 
TGGCGGGACC TCAGTGGTGG GGGGGCACAG CAGCAGGACC TGAGCCCTGG GCAGGGGTCA 
GTGTTTTTAG ATCACTTGAA GCCTGGCACG GACTATGAGG TGACTGTGAG CGCCCTATTT 
GGCCGCAGCG TGGGGCCTGC TGCTTCTCTG ACTACCCGCA CTGCCTCTTC TGTTGAGCAG 
ACACTGCGCC CCATCATCCT GAGCCCCACA TCTATTCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCCT GGAGTGGCAT CGTGAGAGTG GCTTGGAACC ACCACAGAAA 
GTGGAACTGC TCCCTGATGT GACCCGCTAC CAGTTGGATG GGTTGCAGCC AGGCACTGAG 
TATCGCCTCA CGCTCTACAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCGACTGTG 
GTTCCTACTG GGCTAGAGCA GCTGGTGGGC CCGGTGATGA ATTTACAAGC CACAGAACTA 
CCTGGGCAGC GGCTGCGAGT GTCTTGGAAC CCAGTCCTCG GTGCCACCGA GTACCGTTTC 
ACTGTACGCA CCCCCCAGGG GGTCGAGCGG ACTTTGTTGC TTCCTGGGAG TCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCCGGGATT AGCTACACAG TGCGAGTGTC TGCTCGAGTG 
GGCACTCATG AGGGTGATGC CAGCACCCTC ACCATCCACA GAGATCAAGA AGCCCCACTT 
ATTATCCCAG GACTGCAGGT TGTGGCATCA GATGCAACAA GAATTAGAGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATT AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGTCAGACGC TGGCCCCAGA CTCTACTGTC ACAGACATCC TGGGGCTGCA GCCTGGAACC 
TCTTATCAAG TGGCTGTGTC AGCCCTTCGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTCGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGTAGC 
TCATCTGTCA GCATTACCTG GACTGGGGTT CCTGGCGCCA CCGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCAA TTGGTTTCTG GGGACGCCAC GGTGGCTGAA 
ATAGATGGAC TGGAGCCAGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA TGTAGCCGGT 
GTGGATGGAG CCCCGGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT AGGCAGTGTG 
TCAAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTCC GCGTCACTTG GGTGGGAGTC 
CCTGGAGCCA CATCCTATAG ACTGGCTTGG GGACGTAGTG AAGGTGGACC TATGAGACAC 
CAGATAGTCC CAGGAAACAA AGACTCTGCA GAGATAACAG GTCTGGAAGG CGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCCGT GTCCATTGTC 
GTCACCACAC CGCCTGAGGT CCCAGCACCT CTGGAGACCC TTCAGGTAGT GCAGAGTGGG 
GAGCACTCCT TGAGGCTGCA CTGGAACCGA GTCCCCGGAG CACAGGGCTT CCGTCTGCAT 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTCG GACTGGAGCC AGCAACGAAG TACCATGTAT GGCTGAGTGT CCTGGGCCAG 
CCTGGAGAAG CTGCCCCTAG GAAGGTGACT GCTTACACTG AGACTCCTCA CATCCCGAGC 
ACTGGGCTAC GGGTGGTGGA CACCTCTGTC AACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGCTATAT CTTGTCCTGG CGGCCACTCA GAGGAACTGG CCAGGAAGTG 
CCGGGGGGCC CGCAGACACT GCCAGGGAAC TCAAGCTCCC ACAGAGTGAC AGGCCTGGAA 
CCTGGGGTTC CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCACTGTCA CACAAAAGCC AGCGTGTCCC CATGGCCAGG TGGACGTGGT GATCCTGTTA 
CATGCCACTC GAGACAACGC TCACAATGCA GAAGCTGTAA AGAGGTTCCT GGAAAGGCTG 
GTGTCTGCAC TCGGGCCTCT TGGTCCACAG GCTGCTCAGG TTGGCCTGCT GTCCTACAGC 
CACCGGCCCT CCCCACTGTT CCCGCTGAAT AGTTCTCACG ATCTTGGTGT CATCTTGCAA 
AAGATCCGTG ACATCCCCTA TGTGGACCCA AGTGGTAACA ACCTAGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCACCCAAT GCTCCCGGTC GCCGTCGGCA AGTGCCAGGC 
GTGATGGTAC TGTTAGTGGA TGAACCTCTG AGAGAAGACA TATTGAGCCC CATTCGTGAG 
GCCCAGGCTT CTGGGCTCAA GGTGATGATG CTGGGTCTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGCCTCT TGGCACCAGG CATGGACCCC ATCCAGACCT TCTTTGCTGT GGATAATGGC 
TTAGACTTGG ACCGAGCAGG CAGTGATCTG GCTGTAGCCC TATGTCAGGC AGCTGTGGCC 
ATACAGCCAC AACTAGAGCC TTGTGCTGTG CCCTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTGACGG GACCGCAAGG ACAGGCTGGA CCTCCTGGTC CCCCCGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCACA AGGTGCTCCC GGAAGTACCC AAGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGCCC TCCAGGCAGT CCTGGCCTAC CTGGTGTTCC TGGGTCTCCT 
GGCGTTAAGG GCTCCCCAGG GTGGTCTGGC CCTCGTGGGG ATCGGGGAGA ACGAGGGCCT 
CAAGGCCCAA AAGGGGAGCC GGGGGAGCCT GGGCAAGTCA TTGGAGGCGG AAGGCCAGGG 
CTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGACCCC CTGGACCTCA TGGCCCTTTG 
GGGGACCCAG GACCTCGTGG CCCCCCTGGG CTTCCTGGAA CATCAGTGAA GGGTGACAAA 
GGTGACCGTG GGGAACGGGG TCCCCCAGGA CCAGGCACTG GTGCCAGTGA GCAAGGCAGC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCACCTGG TCGAACTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGCCCAG GCCTTCCAGG ACAGCCTGGG 
GTTCCAGGGG AACCGGGCCT GCGGGGAGCT CCTGGAGTTA CTGGGCCCAA AGGTGATCGG 
GGACTGACAG GGACCCCGGG TGAGCCTGGA GAGAAGGGTG AACGGGGACC ACCTGGTCCC 
GTGGGACCCC AGGGGCTTCC CGGAGCTGCT GGACGTCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCCCTGGCCG CCGGGGAGAA AAGGGAGAGC CTGGTCGCCC TGGGGATCCT 
GCATTGGGCC CTGGTGGTGC TGGAGCCAAG GGAGAAAAGG GAGATGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAT CAAAGGAGAG CAGGGTGCAC CTGGGTTGGC TCTTCCTGGA 
GACCCTGGCC CCAAAGGAGA CCCTGGAGAC AGAGGTCCCA TTGGCCTCAC TGGCAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAGGGAG AACCTGGGCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCTCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGT 
GCCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GCGGCCTCCG GGGGGCACCT 
GGGCCCCGGG GGCCTGTGGG TGAGAAGGGG AATGAGGGGG ATCCTGGAGA AGATGGCCGG 
AATGGCACCC CTGGACCATC TGGACCCAAG GGTGACCGTG GGGAGCCGGG CCCCCCGGGT 
CTCCCTGGAC GGCTGGTGGA TGCCGCCCTT GAATCCAGAG ACAAGGGAGA GCCTGGACAG 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGCCCCCCTG GAGCCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CAGGTGTTCG AGGTCCTGCA 
GGAGACAAGG GTGATCGGGG ATCCCCAGGG CTGGATGGCC GAAATGGGCT AGATGGGAAA 
CCAGGAGCCC CTGGCCCTCC AGGGCTACAC GGTGCTTCAG GGAAAGCTGG GGACCCTGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCAC CTGGTCCCCC TGGCCCTCCT 
GGAGTACCGG GAAAGCCAGG CGATGACGGC AAACCCGGCC TGAATGGGAA AAACGGAGAA 
CCTGGGGATC CCGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGTCCCTGGA 
AGGGAAGGTC CTGACGGTCC CAAGGGTGAA CGCGGAGCTC CTGGTAATCC CGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAGTTACAG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCG GGGAGCTTGC CGAATGCGGA GCGGTTCCTG 
GAAACTGCTG GCATCAAGGT GTCAGCCCTG CGGGAGATTG TGGACACCTG GGGTGAGAGT 
TCTGGCAGCT TCCTGCTGGT GCCTGAGCGG AGACAAGGTC CCAAGGGGGA CCCTGGTGAC 
CCAGGCCCTC CAGGCAAGGA GGGTTCCATT GGTCTTCCTG GAGAACGTGG GCTAAAGGGA 
GAACGAGGAG ACCCTGGCCC TCAGGGGCCT CCTGGTCTCG CCCTTGGTGA GAGGGGCCCA 
CCTGGTCCAT CTGGCCTTGC CGGGGAACCA GGAAAGCCTG GAATTCCTGG GCTCCCAGGT 
CGGGCTGGGG CTGCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGAACAGGG CAGAGATGGC CATCCCGGCC TTCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCTA GGTCCTGGAC CCGCTAGAGA ACAAGGACCT 
CCTGGACTCA AGGGTGCTAA GGGGGAGCCA GGCAGTGATG GTGTCCCAGG ACCCAAAGGA 
GACAGGGGTG TACCCGGCAT CAAAGGAGAT GCAGGAGAGC CAGGAAAGAG GGGTCCTGAT 
GGTAACCCAG GTCTGCCAGG AGAGCGCGGT GTGTCTGGAC CTGAAGGGAA GCCGGGTCTG 
CAGGGCCCGA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCAGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCAGGGCCC CAGGGACCTT CTGGCCTGAA GGGGGAGCCC 
GGAGAGACAG GACCCCCAGG ACGGGGTCTG CCTGGACCTA CTGGAGCTGT GGGACTTCCT 
GGCCCTCCTG GCCCTTCAGG CCTCGTGGGC CCACAAGGCT CACCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCCCCA GGCCGGGATG GTAGCAGCGG AAAGGACGGA 
GAAAGAGGAG GCCCTGGCGT GCCAGGGTTA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GACCTGTGGG GGCCCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCAGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAG 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCA TGCAGGAGAG 
CCTGGCGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTCAAGGGC 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGTGCT CCTGGTATTG TTGGATTTCC TGGTCAGCCA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGCGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCGGG GTCAGTGGGA 
GCACCTGGCG CCTCTGGATT CAAAGGAGAC AAGGGAGACT CAGGAGCAGG GCTGCCAGGG 
CCCCGAGGCG AGCGTGGTGA ACCAGGTCTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTCATGGG GCCCCCTGGC AGCCGGGGAG AGCGAGGAGA GAAGGGCGAC 
CCTGGTGCTG CAGGACTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTGCA 
GGGCCACGAG GTGCCAAGGG GGATATGGGT GAACGAGGGC CTCGAGGCAT AGATGGTGAC 
CAAGGACCTC GGGGTGAAAG TGGGGATCCT GGGGACAAGG GCTCCAAAGG AGAGCCTGGA 
GACAAAGGCT CGGCTGGCTC CACTGGAGTT CGCGGACTCA CAGGACCCAA GGCAAGGCGA 
GCCAGGTGCT GCAGGGATCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col7a1 ( XM_006243759.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243821.1 Rattus norvegicus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.

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CloneID	ORa31567
Clone ID Related Accession (Same CDS sequence)	XM_006243759.3
Accession Version	XM_006243759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(VII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GGCTCCTGGT CGCTGCGCTC TGCACTGCCG AGATCCTGGT GGGGGCGCCC 
GGAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACCC GCCTTTATGC TGCTGACATT 
GTGTTCTTGA TTGACGGCTC CTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGGGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA ATGCACAGGG CGTCCGATTT 
GCAACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGGGTGACA CCATTCGTGC CATCCGGGAG CTCAGCTATA AGGGTGGTAA CACTCGAACG 
GGGGCTGCTC TTCTCCACGT TTCCGACCGG GTCTTCCTGC CTCATCTAAC ACGTCCTGGC 
ATCCCCAAGG TCTGTATCCT GATCACAGAT GGGAAATCCC AGGACTTAGT GGACACAGCT 
GCCCAAAAAC TGAAGAGGCA GGGGGTCAAG CTGTTTGCCG TGGGAATCAA GAACGCCGAT 
CCTGAGGAAC TGAAGCGCAT CGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTTGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCC CTCATTTCCC GGAGGGTGTG CACCACGGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCCGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTGTCTGAGC CAAGTAGCCA ATCCTTACGA GTACAGTGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AAATTCAGTA CACGCCCCTG ACGGGGCTAG GACAGCCACT GCCAAGTGAG 
CGAAAGGAGG TGAACGTCCC GGCTGGTGAG ACCAGCATGC GACTACAGGG TCTCCGGCCA 
CTAACTGACT ACCAAGTGAC TGTGGTGGCC CTCTATGCTA ACAGCATCGG AGAGGCTGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAGC TGAGCGTCCA GAACATCACA 
TCCCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGCGTAACA 
TGGCGGGACC TCAGTGGTGG GGGGGCACAG CAGCAGGACC TGAGCCCTGG GCAGGGGTCA 
GTGTTTTTAG ATCACTTGAA GCCTGGCACG GACTATGAGG TGACTGTGAG CGCCCTATTT 
GGCCGCAGCG TGGGGCCTGC TGCTTCTCTG ACTACCCGCA CTGCCTCTTC TGTTGAGCAG 
ACACTGCGCC CCATCATCCT GAGCCCCACA TCTATTCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCCT GGAGTGGCAT CGTGAGAGTG GCTTGGAACC ACCACAGAAA 
GTGGAACTGC TCCCTGATGT GACCCGCTAC CAGTTGGATG GGTTGCAGCC AGGCACTGAG 
TATCGCCTCA CGCTCTACAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCGACTGTG 
GTTCCTACTG GGCTAGAGCA GCTGGTGGGC CCGGTGATGA ATTTACAAGC CACAGAACTA 
CCTGGGCAGC GGCTGCGAGT GTCTTGGAAC CCAGTCCTCG GTGCCACCGA GTACCGTTTC 
ACTGTACGCA CCCCCCAGGG GGTCGAGCGG ACTTTGTTGC TTCCTGGGAG TCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCCGGGATT AGCTACACAG TGCGAGTGTC TGCTCGAGTG 
GGCACTCATG AGGGTGATGC CAGCACCCTC ACCATCCACA GAGATCAAGA AGCCCCACTT 
ATTATCCCAG GACTGCAGGT TGTGGCATCA GATGCAACAA GAATTAGAGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATT AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGTCAGACGC TGGCCCCAGA CTCTACTGTC ACAGACATCC TGGGGCTGCA GCCTGGAACC 
TCTTATCAAG TGGCTGTGTC AGCCCTTCGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTCGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGTAGC 
TCATCTGTCA GCATTACCTG GACTGGGGTT CCTGGCGCCA CCGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCAA TTGGTTTCTG GGGACGCCAC GGTGGCTGAA 
ATAGATGGAC TGGAGCCAGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA TGTAGCCGGT 
GTGGATGGAG CCCCGGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT AGGCAGTGTG 
TCAAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTCC GCGTCACTTG GGTGGGAGTC 
CCTGGAGCCA CATCCTATAG ACTGGCTTGG GGACGTAGTG AAGGTGGACC TATGAGACAC 
CAGATAGTCC CAGGAAACAA AGACTCTGCA GAGATAACAG GTCTGGAAGG CGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCCGT GTCCATTGTC 
GTCACCACAC CGCCTGAGGT CCCAGCACCT CTGGAGACCC TTCAGGTAGT GCAGAGTGGG 
GAGCACTCCT TGAGGCTGCA CTGGAACCGA GTCCCCGGAG CACAGGGCTT CCGTCTGCAT 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTCG GACTGGAGCC AGCAACGAAG TACCATGTAT GGCTGAGTGT CCTGGGCCAG 
CCTGGAGAAG CTGCCCCTAG GAAGGTGACT GCTTACACTG AGACTCCTCA CATCCCGAGC 
ACTGGGCTAC GGGTGGTGGA CACCTCTGTC AACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGCTATAT CTTGTCCTGG CGGCCACTCA GAGGAACTGG CCAGGAAGTG 
CCGGGGGGCC CGCAGACACT GCCAGGGAAC TCAAGCTCCC ACAGAGTGAC AGGCCTGGAA 
CCTGGGGTTC CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCACTGTCA CACAAAAGCC AGCGTGTCCC CATGGCCAGG TGGACGTGGT GATCCTGTTA 
CATGCCACTC GAGACAACGC TCACAATGCA GAAGCTGTAA AGAGGTTCCT GGAAAGGCTG 
GTGTCTGCAC TCGGGCCTCT TGGTCCACAG GCTGCTCAGG TTGGCCTGCT GTCCTACAGC 
CACCGGCCCT CCCCACTGTT CCCGCTGAAT AGTTCTCACG ATCTTGGTGT CATCTTGCAA 
AAGATCCGTG ACATCCCCTA TGTGGACCCA AGTGGTAACA ACCTAGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCACCCAAT GCTCCCGGTC GCCGTCGGCA AGTGCCAGGC 
GTGATGGTAC TGTTAGTGGA TGAACCTCTG AGAGAAGACA TATTGAGCCC CATTCGTGAG 
GCCCAGGCTT CTGGGCTCAA GGTGATGATG CTGGGTCTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGCCTCT TGGCACCAGG CATGGACCCC ATCCAGACCT TCTTTGCTGT GGATAATGGC 
TTAGACTTGG ACCGAGCAGG CAGTGATCTG GCTGTAGCCC TATGTCAGGC AGCTGTGGCC 
ATACAGCCAC AACTAGAGCC TTGTGCTGTG CCCTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTGACGG GACCGCAAGG ACAGGCTGGA CCTCCTGGTC CCCCCGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCACA AGGTGCTCCC GGAAGTACCC AAGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGCCC TCCAGGCAGT CCTGGCCTAC CTGGTGTTCC TGGGTCTCCT 
GGCGTTAAGG GCTCCCCAGG GTGGTCTGGC CCTCGTGGGG ATCGGGGAGA ACGAGGGCCT 
CAAGGCCCAA AAGGGGAGCC GGGGGAGCCT GGGCAAGTCA TTGGAGGCGG AAGGCCAGGG 
CTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGACCCC CTGGACCTCA TGGCCCTTTG 
GGGGACCCAG GACCTCGTGG CCCCCCTGGG CTTCCTGGAA CATCAGTGAA GGGTGACAAA 
GGTGACCGTG GGGAACGGGG TCCCCCAGGA CCAGGCACTG GTGCCAGTGA GCAAGGCAGC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCACCTGG TCGAACTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGCCCAG GCCTTCCAGG ACAGCCTGGG 
GTTCCAGGGG AACCGGGCCT GCGGGGAGCT CCTGGAGTTA CTGGGCCCAA AGGTGATCGG 
GGACTGACAG GGACCCCGGG TGAGCCTGGA GAGAAGGGTG AACGGGGACC ACCTGGTCCC 
GTGGGACCCC AGGGGCTTCC CGGAGCTGCT GGACGTCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCCCTGGCCG CCGGGGAGAA AAGGGAGAGC CTGGTCGCCC TGGGGATCCT 
GCATTGGGCC CTGGTGGTGC TGGAGCCAAG GGAGAAAAGG GAGATGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAT CAAAGGAGAG CAGGGTGCAC CTGGGTTGGC TCTTCCTGGA 
GACCCTGGCC CCAAAGGAGA CCCTGGAGAC AGAGGTCCCA TTGGCCTCAC TGGCAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAGGGAG AACCTGGGCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCTCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGT 
GCCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GCGGCCTCCG GGGGGCACCT 
GGGCCCCGGG GGCCTGTGGG TGAGAAGGGG AATGAGGGGG ATCCTGGAGA AGATGGCCGG 
AATGGCACCC CTGGACCATC TGGACCCAAG GGTGACCGTG GGGAGCCGGG CCCCCCGGGT 
CTCCCTGGAC GGCTGGTGGA TGCCGCCCTT GAATCCAGAG ACAAGGGAGA GCCTGGACAG 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGCCCCCCTG GAGCCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CAGGTGTTCG AGGTCCTGCA 
GGAGACAAGG GTGATCGGGG ATCCCCAGGG CTGGATGGCC GAAATGGGCT AGATGGGAAA 
CCAGGAGCCC CTGGCCCTCC AGGGCTACAC GGTGCTTCAG GGAAAGCTGG GGACCCTGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCAC CTGGTCCCCC TGGCCCTCCT 
GGAGTACCGG GAAAGCCAGG CGATGACGGC AAACCCGGCC TGAATGGGAA AAACGGAGAA 
CCTGGGGATC CCGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGTCCCTGGA 
AGGGAAGGTC CTGACGGTCC CAAGGGTGAA CGCGGAGCTC CTGGTAATCC CGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAGTTACAG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCG GGGAGCTTGC CGAATGCGGA GCGGTTCCTG 
GAAACTGCTG GCATCAAGGT GTCAGCCCTG CGGGAGATTG TGGACACCTG GGGTGAGAGT 
TCTGGCAGCT TCCTGCTGGT GCCTGAGCGG AGACAAGGTC CCAAGGGGGA CCCTGGTGAC 
CCAGGCCCTC CAGGCAAGGA GGGTTCCATT GGTCTTCCTG GAGAACGTGG GCTAAAGGGA 
GAACGAGGAG ACCCTGGCCC TCAGGGGCCT CCTGGTCTCG CCCTTGGTGA GAGGGGCCCA 
CCTGGTCCAT CTGGCCTTGC CGGGGAACCA GGAAAGCCTG GAATTCCTGG GCTCCCAGGT 
CGGGCTGGGG CTGCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGAACAGGG CAGAGATGGC CATCCCGGCC TTCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCTA GGTCCTGGAC CCGCTAGAGA ACAAGGACCT 
CCTGGACTCA AGGGTGCTAA GGGGGAGCCA GGCAGTGATG GTGTCCCAGG ACCCAAAGGA 
GACAGGGGTG TACCCGGCAT CAAAGGAGAT GCAGGAGAGC CAGGAAAGAG GGGTCCTGAT 
GGTAACCCAG GTCTGCCAGG AGAGCGCGGT GTGTCTGGAC CTGAAGGGAA GCCGGGTCTG 
CAGGGCCCGA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCAGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCAGGGCCC CAGGGACCTT CTGGCCTGAA GGGGGAGCCC 
GGAGAGACAG GACCCCCAGG ACGGGGTCTG CCTGGACCTA CTGGAGCTGT GGGACTTCCT 
GGCCCTCCTG GCCCTTCAGG CCTCGTGGGC CCACAAGGCT CACCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCCCCA GGCCGGGATG GTAGCAGCGG AAAGGACGGA 
GAAAGAGGAG GCCCTGGCGT GCCAGGGTTA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GACCTGTGGG GGCCCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCAGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAG 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCA TGCAGGAGAG 
CCTGGCGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTCAAGGGC 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGTGCT CCTGGTATTG TTGGATTTCC TGGTCAGCCA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGCGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCGGG GTCAGTGGGA 
GCACCTGGCG CCTCTGGATT CAAAGGAGAC AAGGGAGACT CAGGAGCAGG GCTGCCAGGG 
CCCCGAGGCG AGCGTGGTGA ACCAGGTCTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTCATGGG GCCCCCTGGC AGCCGGGGAG AGCGAGGAGA GAAGGGCGAC 
CCTGGTGCTG CAGGACTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTGCA 
GGGCCACGAG GTGCCAAGGG GGATATGGGT GAACGAGGGC CTCGAGGCAT AGATGGTGAC 
CAAGGACCTC GGGGTGAAAG TGGGGATCCT GGGGACAAGG GCTCCAAAGG AGAGCCTGGA 
GACAAAGGCT CGGCTGGCTC CACTGGAGTT CGCGGACTCA CAGGACCCAA GGGCGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCGGGAA AGGATGGAGC CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCACG GGTCCCAGGG GTCTCAAGGG TGAAAGAGGA 
GTAAAGGGAA CCTGTGGCCG TGACGGAGAA AAAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGTCAGG AAAGAAAGGA GACATGGGGG ATCCTGGTAT CCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA GGGGCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCGGGGC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCCCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAATGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTAGT 
ATTGGTCCCA AGGGACCTGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGTGTGG TGGGGGCTCC CGGTGCTCCT GGCACCCCTG GTGAGAGAGG GGAACAGGGG 
CGACCGGGAC CTGCCGGCCC CCGAGGGGAG AAAGGAGAAG CTGCACTGAC TGAGGATGAC 
ATTCGGGGCT TTGTGCGTCA GGAGATGAGT CAGCATTGTG CCTGCCAGGG ACAGTTTATT 
GCGTCCGGAT CACGACCCCT CCCTGGTTAT GCTGCGGATA CAGCTGGCCC CCAGCTCCAC 
CATGTTCCTG TGCTCCGGGT CTCTCACGTA GAGGAGGAAG GCCAGGTGCC CCCTGAAGAC 
GATGACGACT TCTCTGAGTA CTCCGTGTAT TCTGTGGAGG ACTACCAGGA CCCTGAAGTT 
TCATGGGATG GTGATGCCGA GACTAAGGGC TGGGACCATA GAGGCTCAGA TCTCTGCTCC 
CTGCCACTGG ATGAGGGTTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTA 
CCTGGTGGCA CAGCGTGTCA TCCCTTTGTC TACGGTGGCT GCAAGGGGAA TGCTAATCGT 
TTTGGAACCC GTGAGGCGTG CGAGCGCCGC TGCCCACCCC AAGGGGTCCA CCGTCAGAAC 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243821.1
CDS	56..8890
Translation	MRLRLLVAALCTAEILVGAPGVWAQPRDRVTCTRLYAADIVFLIDGSSSIGRSNFREVRGFLEGLVLPFSGAANAQGVRFATVQYSDDPQTEFGLDTLGSGGDTIRAIRELSYKGGNTRTGAALLHVSDRVFLPHLTRPGIPKVCILITDGKSQDLVDTAAQKLKRQGVKLFAVGIKNADPEELKRIASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKIQYTPLTGLGQPLPSERKEVNVPAGETSMRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSVQNITSHSLLVAWRRVPGANGYRVTWRDLSGGGAQQQDLSPGQGSVFLDHLKPGTDYEVTVSALFGRSVGPAASLTTRTASSVEQTLRPIILSPTSILLSWNLVPEARGYRLEWHRESGLEPPQKVELLPDVTRYQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVGPVMNLQATELPGQRLRVSWNPVLGATEYRFTVRTPQGVERTLLLPGSQTTFDLDDVRAGISYTVRVSARVGTHEGDASTLTIHRDQEAPLIIPGLQVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSQTLAPDSTVTDILGLQPGTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSQLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYRLAWGRSEGGPMRHQIVPGNKDSAEITGLEGGVSYSVRVTALVGDREGAPVSIVVTTPPEVPAPLETLQVVQSGEHSLRLHWNRVPGAQGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYHVWLSVLGQPGEAAPRKVTAYTETPHIPSTGLRVVDTSVNSVTLTWTPVSGASSYILSWRPLRGTGQEVPGGPQTLPGNSSSHRVTGLEPGVPYVFSLTPIQSGVRGSEITVTQKPACPHGQVDVVILLHATRDNAHNAEAVKRFLERLVSALGPLGPQAAQVGLLSYSHRPSPLFPLNSSHDLGVILQKIRDIPYVDPSGNNLGTAVTTAHRYLLAPNAPGRRRQVPGVMVLLVDEPLREDILSPIREAQASGLKVMMLGLVGADPEQLRLLAPGMDPIQTFFAVDNGLDLDRAGSDLAVALCQAAVAIQPQLEPCAVPCPKGQKGEPGVTGPQGQAGPPGPPGLPGRTGAPGPQGAPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGVKGSPGWSGPRGDRGERGPQGPKGEPGEPGQVIGGGRPGLPGKKGDPGPSGPPGPHGPLGDPGPRGPPGLPGTSVKGDKGDRGERGPPGPGTGASEQGSPGLPGLPGSPGPQGPPGRTGEKGEKGDCEDGGPGLPGQPGVPGEPGLRGAPGVTGPKGDRGLTGTPGEPGEKGERGPPGPVGPQGLPGAAGRPGVEGPEGPPGPPGRRGEKGEPGRPGDPALGPGGAGAKGEKGDAGLPGPRGASGIKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGAPGEPGLPGKAGERGLRGAPGPRGPVGEKGNEGDPGEDGRNGTPGPSGPKGDRGEPGPPGLPGRLVDAALESRDKGEPGQEGPRGPKGDPGPPGASGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGSPGLDGRNGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKPGDDGKPGLNGKNGEPGDPGEDGRKGEKGDSGVPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGVTGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERFLETAGIKVSALREIVDTWGESSGSFLLVPERRQGPKGDPGDPGPPGKEGSIGLPGERGLKGERGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGAAGEAGRPGERGERGEKGERGEQGRDGHPGLPGPPGPPGPKVAIEELGPGPAREQGPPGLKGAKGEPGSDGVPGPKGDRGVPGIKGDAGEPGKRGPDGNPGLPGERGVSGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGERGGPGVPGLPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGHAGEPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGIVGFPGQPGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSVGAPGASGFKGDKGDSGAGLPGPRGERGEPGLRGEDGHPGQEGPRGLMGPPGSRGERGEKGDPGAAGLKGDKGDSAVIEGPAGPRGAKGDMGERGPRGIDGDQGPRGESGDPGDKGSKGEPGDKGSAGSTGVRGLTGPKGEPGAAGIPGEPGAPGKDGAPGFRGDKGDIGFTGPRGLKGERGVKGTCGRDGEKGDKGEAGFPGRPGLSGKKGDMGDPGIPGQSGAPGKEGLIGPKGDRGFDGQSGPKGDQGEKGERGPPGVGGFPGPRGNDGSSGPPGPPGSIGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRGFVRQEMSQHCACQGQFIASGSRPLPGYAADTAGPQLHHVPVLRVSHVEEEGQVPPEDDDDFSEYSVYSVEDYQDPEVSWDGDAETKGWDHRGSDLCSLPLDEGSCTAYTLRWYHRAVPGGTACHPFVYGGCKGNANRFGTREACERRCPPQGVHRQNTGAA

Target ORF information:

RefSeq Version	XM_006243759.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243759.3

ORF Insert Sequence:

ATGAGGCTGC GGCTCCTGGT CGCTGCGCTC TGCACTGCCG AGATCCTGGT GGGGGCGCCC 
GGAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACCC GCCTTTATGC TGCTGACATT 
GTGTTCTTGA TTGACGGCTC CTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGGGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA ATGCACAGGG CGTCCGATTT 
GCAACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGGGTGACA CCATTCGTGC CATCCGGGAG CTCAGCTATA AGGGTGGTAA CACTCGAACG 
GGGGCTGCTC TTCTCCACGT TTCCGACCGG GTCTTCCTGC CTCATCTAAC ACGTCCTGGC 
ATCCCCAAGG TCTGTATCCT GATCACAGAT GGGAAATCCC AGGACTTAGT GGACACAGCT 
GCCCAAAAAC TGAAGAGGCA GGGGGTCAAG CTGTTTGCCG TGGGAATCAA GAACGCCGAT 
CCTGAGGAAC TGAAGCGCAT CGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTTGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCC CTCATTTCCC GGAGGGTGTG CACCACGGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCCGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTGTCTGAGC CAAGTAGCCA ATCCTTACGA GTACAGTGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AAATTCAGTA CACGCCCCTG ACGGGGCTAG GACAGCCACT GCCAAGTGAG 
CGAAAGGAGG TGAACGTCCC GGCTGGTGAG ACCAGCATGC GACTACAGGG TCTCCGGCCA 
CTAACTGACT ACCAAGTGAC TGTGGTGGCC CTCTATGCTA ACAGCATCGG AGAGGCTGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAGC TGAGCGTCCA GAACATCACA 
TCCCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGCGTAACA 
TGGCGGGACC TCAGTGGTGG GGGGGCACAG CAGCAGGACC TGAGCCCTGG GCAGGGGTCA 
GTGTTTTTAG ATCACTTGAA GCCTGGCACG GACTATGAGG TGACTGTGAG CGCCCTATTT 
GGCCGCAGCG TGGGGCCTGC TGCTTCTCTG ACTACCCGCA CTGCCTCTTC TGTTGAGCAG 
ACACTGCGCC CCATCATCCT GAGCCCCACA TCTATTCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCCT GGAGTGGCAT CGTGAGAGTG GCTTGGAACC ACCACAGAAA 
GTGGAACTGC TCCCTGATGT GACCCGCTAC CAGTTGGATG GGTTGCAGCC AGGCACTGAG 
TATCGCCTCA CGCTCTACAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCGACTGTG 
GTTCCTACTG GGCTAGAGCA GCTGGTGGGC CCGGTGATGA ATTTACAAGC CACAGAACTA 
CCTGGGCAGC GGCTGCGAGT GTCTTGGAAC CCAGTCCTCG GTGCCACCGA GTACCGTTTC 
ACTGTACGCA CCCCCCAGGG GGTCGAGCGG ACTTTGTTGC TTCCTGGGAG TCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCCGGGATT AGCTACACAG TGCGAGTGTC TGCTCGAGTG 
GGCACTCATG AGGGTGATGC CAGCACCCTC ACCATCCACA GAGATCAAGA AGCCCCACTT 
ATTATCCCAG GACTGCAGGT TGTGGCATCA GATGCAACAA GAATTAGAGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATT AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGTCAGACGC TGGCCCCAGA CTCTACTGTC ACAGACATCC TGGGGCTGCA GCCTGGAACC 
TCTTATCAAG TGGCTGTGTC AGCCCTTCGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTCGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGTAGC 
TCATCTGTCA GCATTACCTG GACTGGGGTT CCTGGCGCCA CCGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCAA TTGGTTTCTG GGGACGCCAC GGTGGCTGAA 
ATAGATGGAC TGGAGCCAGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA TGTAGCCGGT 
GTGGATGGAG CCCCGGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT AGGCAGTGTG 
TCAAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTCC GCGTCACTTG GGTGGGAGTC 
CCTGGAGCCA CATCCTATAG ACTGGCTTGG GGACGTAGTG AAGGTGGACC TATGAGACAC 
CAGATAGTCC CAGGAAACAA AGACTCTGCA GAGATAACAG GTCTGGAAGG CGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCCGT GTCCATTGTC 
GTCACCACAC CGCCTGAGGT CCCAGCACCT CTGGAGACCC TTCAGGTAGT GCAGAGTGGG 
GAGCACTCCT TGAGGCTGCA CTGGAACCGA GTCCCCGGAG CACAGGGCTT CCGTCTGCAT 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTCG GACTGGAGCC AGCAACGAAG TACCATGTAT GGCTGAGTGT CCTGGGCCAG 
CCTGGAGAAG CTGCCCCTAG GAAGGTGACT GCTTACACTG AGACTCCTCA CATCCCGAGC 
ACTGGGCTAC GGGTGGTGGA CACCTCTGTC AACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGCTATAT CTTGTCCTGG CGGCCACTCA GAGGAACTGG CCAGGAAGTG 
CCGGGGGGCC CGCAGACACT GCCAGGGAAC TCAAGCTCCC ACAGAGTGAC AGGCCTGGAA 
CCTGGGGTTC CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCACTGTCA CACAAAAGCC AGCGTGTCCC CATGGCCAGG TGGACGTGGT GATCCTGTTA 
CATGCCACTC GAGACAACGC TCACAATGCA GAAGCTGTAA AGAGGTTCCT GGAAAGGCTG 
GTGTCTGCAC TCGGGCCTCT TGGTCCACAG GCTGCTCAGG TTGGCCTGCT GTCCTACAGC 
CACCGGCCCT CCCCACTGTT CCCGCTGAAT AGTTCTCACG ATCTTGGTGT CATCTTGCAA 
AAGATCCGTG ACATCCCCTA TGTGGACCCA AGTGGTAACA ACCTAGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCACCCAAT GCTCCCGGTC GCCGTCGGCA AGTGCCAGGC 
GTGATGGTAC TGTTAGTGGA TGAACCTCTG AGAGAAGACA TATTGAGCCC CATTCGTGAG 
GCCCAGGCTT CTGGGCTCAA GGTGATGATG CTGGGTCTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGCCTCT TGGCACCAGG CATGGACCCC ATCCAGACCT TCTTTGCTGT GGATAATGGC 
TTAGACTTGG ACCGAGCAGG CAGTGATCTG GCTGTAGCCC TATGTCAGGC AGCTGTGGCC 
ATACAGCCAC AACTAGAGCC TTGTGCTGTG CCCTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTGACGG GACCGCAAGG ACAGGCTGGA CCTCCTGGTC CCCCCGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCACA AGGTGCTCCC GGAAGTACCC AAGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGCCC TCCAGGCAGT CCTGGCCTAC CTGGTGTTCC TGGGTCTCCT 
GGCGTTAAGG GCTCCCCAGG GTGGTCTGGC CCTCGTGGGG ATCGGGGAGA ACGAGGGCCT 
CAAGGCCCAA AAGGGGAGCC GGGGGAGCCT GGGCAAGTCA TTGGAGGCGG AAGGCCAGGG 
CTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGACCCC CTGGACCTCA TGGCCCTTTG 
GGGGACCCAG GACCTCGTGG CCCCCCTGGG CTTCCTGGAA CATCAGTGAA GGGTGACAAA 
GGTGACCGTG GGGAACGGGG TCCCCCAGGA CCAGGCACTG GTGCCAGTGA GCAAGGCAGC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCACCTGG TCGAACTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGCCCAG GCCTTCCAGG ACAGCCTGGG 
GTTCCAGGGG AACCGGGCCT GCGGGGAGCT CCTGGAGTTA CTGGGCCCAA AGGTGATCGG 
GGACTGACAG GGACCCCGGG TGAGCCTGGA GAGAAGGGTG AACGGGGACC ACCTGGTCCC 
GTGGGACCCC AGGGGCTTCC CGGAGCTGCT GGACGTCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCCCTGGCCG CCGGGGAGAA AAGGGAGAGC CTGGTCGCCC TGGGGATCCT 
GCATTGGGCC CTGGTGGTGC TGGAGCCAAG GGAGAAAAGG GAGATGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAT CAAAGGAGAG CAGGGTGCAC CTGGGTTGGC TCTTCCTGGA 
GACCCTGGCC CCAAAGGAGA CCCTGGAGAC AGAGGTCCCA TTGGCCTCAC TGGCAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAGGGAG AACCTGGGCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCTCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGT 
GCCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GCGGCCTCCG GGGGGCACCT 
GGGCCCCGGG GGCCTGTGGG TGAGAAGGGG AATGAGGGGG ATCCTGGAGA AGATGGCCGG 
AATGGCACCC CTGGACCATC TGGACCCAAG GGTGACCGTG GGGAGCCGGG CCCCCCGGGT 
CTCCCTGGAC GGCTGGTGGA TGCCGCCCTT GAATCCAGAG ACAAGGGAGA GCCTGGACAG 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGCCCCCCTG GAGCCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CAGGTGTTCG AGGTCCTGCA 
GGAGACAAGG GTGATCGGGG ATCCCCAGGG CTGGATGGCC GAAATGGGCT AGATGGGAAA 
CCAGGAGCCC CTGGCCCTCC AGGGCTACAC GGTGCTTCAG GGAAAGCTGG GGACCCTGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCAC CTGGTCCCCC TGGCCCTCCT 
GGAGTACCGG GAAAGCCAGG CGATGACGGC AAACCCGGCC TGAATGGGAA AAACGGAGAA 
CCTGGGGATC CCGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGTCCCTGGA 
AGGGAAGGTC CTGACGGTCC CAAGGGTGAA CGCGGAGCTC CTGGTAATCC CGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAGTTACAG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCG GGGAGCTTGC CGAATGCGGA GCGGTTCCTG 
GAAACTGCTG GCATCAAGGT GTCAGCCCTG CGGGAGATTG TGGACACCTG GGGTGAGAGT 
TCTGGCAGCT TCCTGCTGGT GCCTGAGCGG AGACAAGGTC CCAAGGGGGA CCCTGGTGAC 
CCAGGCCCTC CAGGCAAGGA GGGTTCCATT GGTCTTCCTG GAGAACGTGG GCTAAAGGGA 
GAACGAGGAG ACCCTGGCCC TCAGGGGCCT CCTGGTCTCG CCCTTGGTGA GAGGGGCCCA 
CCTGGTCCAT CTGGCCTTGC CGGGGAACCA GGAAAGCCTG GAATTCCTGG GCTCCCAGGT 
CGGGCTGGGG CTGCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGAACAGGG CAGAGATGGC CATCCCGGCC TTCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCTA GGTCCTGGAC CCGCTAGAGA ACAAGGACCT 
CCTGGACTCA AGGGTGCTAA GGGGGAGCCA GGCAGTGATG GTGTCCCAGG ACCCAAAGGA 
GACAGGGGTG TACCCGGCAT CAAAGGAGAT GCAGGAGAGC CAGGAAAGAG GGGTCCTGAT 
GGTAACCCAG GTCTGCCAGG AGAGCGCGGT GTGTCTGGAC CTGAAGGGAA GCCGGGTCTG 
CAGGGCCCGA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCAGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCAGGGCCC CAGGGACCTT CTGGCCTGAA GGGGGAGCCC 
GGAGAGACAG GACCCCCAGG ACGGGGTCTG CCTGGACCTA CTGGAGCTGT GGGACTTCCT 
GGCCCTCCTG GCCCTTCAGG CCTCGTGGGC CCACAAGGCT CACCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCCCCA GGCCGGGATG GTAGCAGCGG AAAGGACGGA 
GAAAGAGGAG GCCCTGGCGT GCCAGGGTTA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GACCTGTGGG GGCCCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCAGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAG 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCA TGCAGGAGAG 
CCTGGCGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTCAAGGGC 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGTGCT CCTGGTATTG TTGGATTTCC TGGTCAGCCA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGCGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCGGG GTCAGTGGGA 
GCACCTGGCG CCTCTGGATT CAAAGGAGAC AAGGGAGACT CAGGAGCAGG GCTGCCAGGG 
CCCCGAGGCG AGCGTGGTGA ACCAGGTCTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTCATGGG GCCCCCTGGC AGCCGGGGAG AGCGAGGAGA GAAGGGCGAC 
CCTGGTGCTG CAGGACTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTGCA 
GGGCCACGAG GTGCCAAGGG GGATATGGGT GAACGAGGGC CTCGAGGCAT AGATGGTGAC 
CAAGGACCTC GGGGTGAAAG TGGGGATCCT GGGGACAAGG GCTCCAAAGG AGAGCCTGGA 
GACAAAGGCT CGGCTGGCTC CACTGGAGTT CGCGGACTCA CAGGACCCAA GGGCGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCGGGAA AGGATGGAGC CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCACG GGTCCCAGGG GTCTCAAGGG TGAAAGAGGA 
GTAAAGGGAA CCTGTGGCCG TGACGGAGAA AAAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGTCAGG AAAGAAAGGA GACATGGGGG ATCCTGGTAT CCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA GGGGCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCGGGGC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCCCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAATGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTAGT 
ATTGGTCCCA AGGGACCTGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGTGTGG TGGGGGCTCC CGGTGCTCCT GGCACCCCTG GTGAGAGAGG GGAACAGGGG 
CGACCGGGAC CTGCCGGCCC CCGAGGGGAG AAAGGAGAAG CTGCACTGAC TGAGGATGAC 
ATTCGGGGCT TTGTGCGTCA GGAGATGAGT CAGCATTGTG CCTGCCAGGG ACAGTTTATT 
GCGTCCGGAT CACGACCCCT CCCTGGTTAT GCTGCGGATA CAGCTGGCCC CCAGCTCCAC 
CATGTTCCTG TGCTCCGGGT CTCTCACGTA GAGGAGGAAG GCCAGGTGCC CCCTGAAGAC 
GATGACGACT TCTCTGAGTA CTCCGTGTAT TCTGTGGAGG ACTACCAGGA CCCTGAAGTT 
TCATGGGATG GTGATGCCGA GACTAAGGGC TGGGACCATA GAGGCTCAGA TCTCTGCTCC 
CTGCCACTGG ATGAGGGTTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTA 
CCTGGTGGCA CAGCGTGTCA TCCCTTTGTC TACGGTGGCT GCAAGGGGAA TGCTAATCGT 
TTTGGAACCC GTGAGGCGTG CGAGCGCCGC TGCCCACCCC AAGGGGTCCA CCGTCAGAAC 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K

Rat model for dominant dystrophic epidermolysis bullosa: glycine substitution reduces collagen VII stability and shows gene-dosage effect.
PloS one8(5)e64243(2013)
Nystr?m A,Buttgereit J,Bader M,Shmidt T,Ozcelik C,Hausser I,Bruckner-Tuderman L,Kern JS

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Insight into interactions of the von-Willebrand-factor-A-like domain 2 with the FNIII-like domain 9 of collagen VII by NMR and SPR.
FEBS letters585(12)1748-52(2011 Jun)
Leineweber S,Sch?nig S,Seeger K

Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment.
Matrix biology : journal of the International Society for Matrix Biology28(8)456-62(2009 Oct)
Gebauer JM,Keene DR,Olsen BR,Sorokin LM,Paulsson M,Wagener R

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Col7a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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