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Tbx5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Tbx5
Entrez Gene ID 304514
Full Name T-box 5
General protein information
Preferred Names

T-box 5

Names

T-box transcription factor TBX5
T-box protein 5
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

12

12q16

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_008769230.2 XP_008767452.1 T-box transcription factor TBX5 isoform X1
XM_006249390.3 XP_006249452.1 T-box transcription factor TBX5 isoform X1
XM_006249388.3 XP_006249450.1 T-box transcription factor TBX5 isoform X1
XM_006249389.3 XP_006249451.1 T-box transcription factor TBX5 isoform X1
XM_006249391.3 XP_006249453.1 T-box transcription factor TBX5 isoform X1
XM_006249392.2 XP_006249454.1 T-box transcription factor TBX5 isoform X1
XM_017598338.1 XP_017453827.1 T-box transcription factor TBX5 isoform X1
XM_006249387.3 XP_006249449.1 T-box transcription factor TBX5 isoform X1
XM_017598339.1 XP_017453828.1 T-box transcription factor TBX5 isoform X1
NM_001009964.1 NP_001009964.1 T-box transcription factor TBX5

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) TBX5 XP_001154140.2
Homo sapiens (human) TBX5 NP_000183.2
Canis lupus familiaris (dog) TBX5 XP_005636327.1
Gallus gallus (chicken) TBX5 NP_989504.1
Mus musculus (house mouse) Tbx5 NP_035667.1
TBX5 XP_001111737.1
Bos taurus (cattle) TBX5 NP_001179678.1
Danio rerio (zebrafish) tbx5a NP_570990.1
Xenopus tropicalis (tropical clawed frog) tbx5 NP_001185697.1
Rattus norvegicus (Norway rat) Tbx5 NP_001009964.1

Gene Ontology


Bibliography

Related articles in PubMed

Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development.
Ghosh TK, Aparicio-S?nchez JJ, Buxton S, Ketley A, Mohamed T, Rutland CS, Loughna S, Brook JD
Journal of molecular and cellular cardiology114185-198(2017 Nov)

Expression of T-box transcription factors 2, 4 and 5 is decreased in the branching airway mesenchyme of nitrofen-induced hypoplastic lungs.
Takahashi T, Friedmacher F, Zimmer J, Puri P
Pediatric surgery international33(2)139-143(2017 Feb)

microRNA-10a Targets T-box 5 to Inhibit the Development of Cardiac Hypertrophy.
Wang D, Zhai G, Ji Y, Jing H
International heart journal58(1)100-106(2017 Feb)

TBX5 loss-of-function mutation contributes to atrial fibrillation and atypical Holt-Oram syndrome.
Guo DF, Li RG, Yuan F, Shi HY, Hou XM, Qu XK, Xu YJ, Zhang M, Liu X, Jiang JQ, Yang YQ, Qiu XB
Molecular medicine reports13(5)4349-56(2016 May)

Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5.
Pradhan L, Gopal S, Li S, Ashur S, Suryanarayanan S, Kasahara H, Nam HJ
Biochemistry55(12)1702-10(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tbx2, Tbx4 and Tbx5 are expressed throughout the mesenchyme of the developing lung, regulating the process of lung branching morphogenesis [12].
Title: Expression of T-box transcription factors 2, 4 and 5 is decreased in the branching airway mesenchyme of nitrofen-induced hypoplastic lungs.

miR-10a may inhibit cardiac hypertrophy via targeting Tbx5.
Title: microRNA-10a Targets T-box 5 to Inhibit the Development of Cardiac Hypertrophy.

exogenous expression of cardiac-specific transcription factors GATA4 and TBX5 enhance cardiomyogenic differentiation bone marrow mesenchymal stromal cells
Title: Transcription factors GATA4 and TBX5 promote cardiomyogenic differentiation of rat bone marrow mesenchymal stromal cells.

TBX5 and MEF2C physically associate through their DNA-binding domains to form a complex on the MYH6 promoter
Title: Physical interaction between TBX5 and MEF2C is required for early heart development.

Findings provide a good basis for further identification of TBX5-related transcription factors and protein-protein interaction studies among each other.
Title: Cloning of TBX5, a key gene during heart formation and its expression in rat embryonic heart.

The following Tbx5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbx5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12974 NM_001009964.1
Latest version!		 Rattus norvegicus T-box transcription factor 5 (Tbx5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249387.3
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_017598338.1
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249388.3
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_017598339.1
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249390.3
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249389.3
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249391.3
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_008769230.2
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
ORa35057 XM_006249392.2
Latest version!		 Rattus norvegicus T-box 5 (Tbx5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12974
Clone ID Related Accession (Same CDS sequence) NM_001009964.1
Accession Version NM_001009964.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY859491.1. On Jan 22, 2005 this sequence version replaced XM_222201.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY859491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
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241
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCAGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGTACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CGAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCCGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACTG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001009964.1
CDS21..1574
Translation

Target ORF information:

RefSeq Version NM_001009964.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box transcription factor 5 (Tbx5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001009964.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCAGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGTACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CGAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCCGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACTG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_008769230.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008767452.1
CDS670..2223
Translation

Target ORF information:

RefSeq Version XM_008769230.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008769230.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249390.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249452.1
CDS692..2245
Translation

Target ORF information:

RefSeq Version XM_006249390.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249390.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249388.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249450.1
CDS647..2200
Translation

Target ORF information:

RefSeq Version XM_006249388.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249388.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249389.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249451.1
CDS822..2375
Translation

Target ORF information:

RefSeq Version XM_006249389.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249389.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249391.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249453.1
CDS397..1950
Translation

Target ORF information:

RefSeq Version XM_006249391.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249391.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249392.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249454.1
CDS210..1763
Translation

Target ORF information:

RefSeq Version XM_006249392.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249392.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_017598338.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017453827.1
CDS295..1848
Translation

Target ORF information:

RefSeq Version XM_017598338.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017598338.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_006249387.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006249449.1
CDS517..2070
Translation

Target ORF information:

RefSeq Version XM_006249387.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006249387.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa35057
Clone ID Related Accession (Same CDS sequence) XM_008769230.2 , XM_006249390.3 , XM_006249388.3 , XM_006249389.3 , XM_006249391.3 , XM_006249392.2 , XM_017598338.1 , XM_006249387.3 , XM_017598339.1
Accession Version XM_017598339.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product T-box transcription factor TBX5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017453828.1
CDS249..1802
Translation

Target ORF information:

RefSeq Version XM_017598339.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus T-box 5 (Tbx5), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017598339.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGATGAGGG CTTCGGCCTG GCGCGCACGC CTCTGGAGCC TGATTCCAAA 
GACAGGTCCT GCGATTCGAA ACCAGAGAGT GCGCTGGGGG CTCCCAGCAA GTCTCCATCC 
TCACCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAAGGAA TCAAGGTGTT TCTTCACGAA 
AGAGAACTGT GGCTGAAGTT CCACGAAGTG GGCACAGAAA TGATCATCAC CAAGGCAGGG 
AGGCGGATGT TTCCTAGCTA CAAAGTGAAG GTGACTGGCC TTAATCCCAA GACGAAGTAC 
ATCCTTCTCA TGGATATTGT ACCCGCAGAC GACCACAGAT ATAAATTTGC TGATAACAAA 
TGGTCCGTAA CTGGTAAAGC CGAGCCTGCT ATGCCGGGTC GCCTTTATGT ACATCCGGAC 
TCCCCAGCAA CCGGAGCGCA CTGGATGCGA CAACTTGTCT CCTTCCAGAA ACTCAAGCTC 
ACCAACAACC ACCTGGACCC ATTCGGACAC ATTATCCTGA ACTCCATGCA CAAATACCAG 
CCCCGATTAC ACATCGTGAA AGCAGACGAA AATAATGGGT TTGGTTCAAA GAATACTGCG 
TTTTGCACCC ACGTCTTTCC GGAGACGGCT TTTATCGCTG TGACTTCGTA CCAGAATCAC 
AAGATCACAC AGCTGAAAAT TGAGAACAAC CCCTTCGCCA AAGGCTTTCG GGGCAGTGAC 
GACCTGGAGT TACACAGGAT GTCTCGGATG CAAAGTAAAG AGTATCCTGT GGTTCCCAGG 
AGCACCGTGA GGCACAAAGT GGCCAACCAC AGCCCCTTCA GCAGCGAGAC CCGAGCTCTC 
TCCACTTCGT CTAACTTAGG GTCCCAGTAC CAGTGTGAGA ATGGTGTCTC CGGCCCCTCC 
CAGGACCTTC TGCCCCCACC TAACCCATAC CCACTGGCCC AGGAGCACAG CCAAATTTAC 
CACTGTACCA AGAGGAAAGA TGAAGAATGT TCCAGCACCG AGCACCCCTA TAAGAAGCCG 
TACATGGAGA CCTCCCCCAG CGAGGAGGAC ACCTTCTACC GCTCTGGCTA CCCCCAGCAG 
CAGGGCCTGA GTACCTCGTA CAGGACAGAG TCAGCCCAGC GGCAGGCCTG CATGTATGCC 
AGCTCCGCTC CACCCAGCGA GCCCGTGCCT AGCCTGGAGG ACATCAGCTG TAACACATGG 
CCCAGCATGC CTTCCTATAG CAGCTGTACA GTTACCACCG TGCAGCCCAT GGACCGTCTC 
CCCTACCAGC ACTTCTCTGC TCATTTCACC TCGGGGCCCC TGGTCCCTCG GCTAGCTGGC 
ATGGCCAACC ATGGTTCTCC CCAGCTTGGT GAAGGGATGT TCCAGCACCA GACCTCAGTG 
ACCCATCAGC CTGTGGTCAG GCAATGCGGG CCTCAGACTG GCCTTCAGTC TCCAGGCAGC 
CTCCAGCCCC CTGAGTTCCT CTACACTCAC GGCGTGCCCA GGACCCTGTC TCCCCACCAG 
TATCACTCGG TACACGGCGT CGGCATGGTG CCAGAGTGGA GCGAGAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Acetylation of TBX5 by KAT2B and KAT2A regulates heart and limb development.
Journal of molecular and cellular cardiology114185-198(2017 Nov)
Ghosh TK,Aparicio-S?nchez JJ,Buxton S,Ketley A,Mohamed T,Rutland CS,Loughna S,Brook JD


book

Expression of T-box transcription factors 2, 4 and 5 is decreased in the branching airway mesenchyme of nitrofen-induced hypoplastic lungs.
Pediatric surgery international33(2)139-143(2017 Feb)
Takahashi T,Friedmacher F,Zimmer J,Puri P


book

microRNA-10a Targets T-box 5 to Inhibit the Development of Cardiac Hypertrophy.
International heart journal58(1)100-106(2017 Feb)
Wang D,Zhai G,Ji Y,Jing H


book

TBX5 loss-of-function mutation contributes to atrial fibrillation and atypical Holt-Oram syndrome.
Molecular medicine reports13(5)4349-56(2016 May)
Guo DF,Li RG,Yuan F,Shi HY,Hou XM,Qu XK,Xu YJ,Zhang M,Liu X,Jiang JQ,Yang YQ,Qiu XB


book

Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5.
Biochemistry55(12)1702-10(2016 Mar)
Pradhan L,Gopal S,Li S,Ashur S,Suryanarayanan S,Kasahara H,Nam HJ