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Lamb3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Lamb3
Entrez Gene ID 305078
Full Name laminin subunit beta 3
General protein information
Preferred Names

laminin subunit beta 3

Names

laminin subunit beta-3
laminin chain
laminin, beta 3
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

13

13q27

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_008769856.2 XP_008768078.1 laminin subunit beta-3 isoform X1
NM_001100841.1 NP_001094311.1 laminin subunit beta-3 precursor
XM_017598839.1 XP_017454328.1 laminin subunit beta-3 isoform X2

Pathways from BioSystems


Homologies


Gallus gallus (chicken) LAMB3 XP_425827.3
Xenopus tropicalis (tropical clawed frog) lamb3 XP_002933550.2
Homo sapiens (human) LAMB3 NP_001017402.1
Pan troglodytes (chimpanzee) LAMB3 XP_003949737.1
LAMB3 XP_002802197.1
Canis lupus familiaris (dog) LAMB3 XP_005622385.1
Bos taurus (cattle) LAMB3 NP_001075065.1
Rattus norvegicus (Norway rat) Lamb3 NP_001094311.1
Mus musculus (house mouse) Lamb3 NP_001264857.1

Gene Ontology


Bibliography

Related articles in PubMed

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

Epitope mapping of function-blocking monoclonal antibody CM6 suggests a "weak" integrin binding site on the laminin-332 LG2 domain.
Yamashita H, Shang M, Tripathi M, Jourquin J, Georgescu W, Liu S, Weidow B, Quaranta V
Journal of cellular physiology223(3)541-8(2010 Jun)

Laminin-332-beta1 integrin interactions negatively regulate invadopodia.
Liu S, Yamashita H, Weidow B, Weaver AM, Quaranta V
Journal of cellular physiology223(1)134-42(2010 Apr)

Transcriptome-based systematic identification of extracellular matrix proteins.
Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)

Wdnm1-like, a new adipokine with a role in MMP-2 activation.
Wu Y, Smas CM
American journal of physiology. Endocrinology and metabolism295(1)E205-15(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Binding sites for alpha3beta1 and alpha6beta4 are closely spaced in the Ln-332 LG domains where they regulate alternative cell functions, namely adhesion/migration or hemidesmosome anchoring.
Title: Epitope mapping of function-blocking monoclonal antibody CM6 suggests a "weak" integrin binding site on the laminin-332 LG2 domain.

In bladder carcinoma laminin-332 can suppress the number of invadopodia that form by interacting with a3b1 integrin and altering the cellular balance of FAK and Src kinase activity.
Title: Laminin-332-beta1 integrin interactions negatively regulate invadopodia.

a complex of laminin alpha 3, beta3 and gamma3 chains serves as the ligand for alpha 6beta1-integrin at the apical ectoplasmic specialization in adult rat testes
Title: Laminin alpha 3 forms a complex with beta3 and gamma3 chains that serves as the ligand for alpha 6beta1-integrin at the apical ectoplasmic specialization in adult rat testes.

The following Lamb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lamb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa58090 XM_017598839.1
Latest version!		 Rattus norvegicus laminin subunit beta 3 (Lamb3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
ORa11134 NM_001100841.1
Latest version!		 Rattus norvegicus laminin subunit beta 3 (Lamb3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa11134 XM_008769856.2
Latest version!		 Rattus norvegicus laminin subunit beta 3 (Lamb3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa58090
Clone ID Related Accession (Same CDS sequence) XM_017598839.1
Accession Version XM_017598839.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product laminin subunit beta-3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTGTGGTA AGAGAAATCC AGGTGGCAAT GCCCAGAATG GAAAATGGCT GTGGCAGATG 
AAATGCTGCA AGTGTGACTC CAGGCTGCCT CGCAACTACA ACAGTCACCG AGTGGAGAAC 
GTTGTATCGT CTTCAGGCCC CATGCGCTGG TGGCAGTCCC AGAACGATGT GAGCCCTGTC 
TCTCTTCAAC TGGACCTAGA CAAGAGGATG CAGCTTCAGG ACATAATGAT GGATTTTAAG 
GGTCTCATGC CAGCTGGCAT GTTGATTGAG CGCTCTTCTG ACTTTGGCAA GACATGGAGG 
GTGTACCAGT ACCTGGCGAC AGACTGTGCC AGCACCTTTC CCCAGGTCCA CCAGGGCCAG 
CCCAAGAACT GGCAGGACGT CCGGTGCCGG CCCTTGTCCC AGAGGCCTAA TGGGCATCTG 
ACTGGGGGAA AGGTCCAACT TAACCTTATG GATTTGGCAT CAGCTATCCC TGCATCTCAA 
AGTAAAAAGA TTCAAGAACT AGGAGACATC ACGAACTTGA GAGTCAACTT CACCAAGCTG 
GCCCCAGTGC CCCAGAGGGG CTCCTATCCA CCCAGCGCCT ACTTTGCAGT ATCACAGCTA 
CGTCTACAGG GGAGTTGCTT CTGTCATGGA CATGCCGACC GCTGTGCCCC TAATCCTGGA 
GGCCCTACAG GTTCCACCAC CGCTGTGCAG GTCAACGATG TCTGTGTCTG CCAGCACAAC 
ACAGCTGGCC CCAACTGTGA CCGCTGCGCC CCCTTCTACA ACAACCGGCC ATGGAGACCT 
GCAGAGGGAC AGGACATCCA TGAGTGCCAA AGGTGTGACT GCAATGGGCA CTCAGAGACC 
TGCCACTTCG ACCCAGCTGT GTTTGCTGCT AGCCAAGGGA CAAGTGGAGG CGTGTGTGAC 
AACTGCCAGG ACCACACAGA GGGCAAGAAC TGTGAGCGAT GTCAGCTACA CTATTTCCGG 
AATAGACGAC CAGGCGCTCC CATTCATGAG ACCTGTATTC CCTGCGAGTG TGATCCAGAC 
GGGGCAGTCC CAGGAGCTCC CTGTGACCGA CTGACGGGCC AGTGCGTGTG CAAAGACCAC 
GTGCAAGGGG AGCGCTGTGA TCTTTGCAAG CCTGGTTTCA CAGGGCTCAC CTTCGCCAAC 
CCACAGGGCT GCCATCCCTG TGACTGCAGC ATCTTAGGTA CCCGGCAAGA TATGCCTTGT 
GAGGAGGAAA CTGGACGCTG CTTGTGTCTT CCCAATGTAG TGGGCCCCAA ATGTGACCAG 
TGCGCCCCTT CCCACTGGAA GCTGGCCAGC GGCCGGGGCT GTGAGCCCTG TGCCTGTGAC 
CCACGCAACT CCCTCAGCTC CCAGTGCAAC CAGTTTACAG GGCAGTGTCC TTGTCGGGAA 
GGGTTTGGTG GCCTCACATG TAGTTCTGCA GCCATTCGTC AGTGTCCCGA CCGGACCCAT 
GGAGACGTGG CCACAGGGTG CCGAGCCTGT GACTGTGACT TCAGAGGAAC CGAGGGTCCT 
GGCTGTGACA AAACCTCAGG CCGTTGCCTC TGCCGCCCCG GCTTGACAGG GCCCCGCTGC 
GACCAGTGCC AACGAGGCTA CTGCGACCGC TACCCAGTGT GTGTGGCCTG CCACTCTTGC 
TTCCAGGCCT ATGACACAGA CCTCCAGGAG CAGGCTTGGC GCCTTGGCAG TCTCCGTAAT 
ACCACTGAAG GCCTGGGCAC AGGGACAGGT CTGGAGGACC GTGGCCTGGC TTCTCGGTTG 
CTAGATGCCA AGAGCAAAAT CGAGCAGATC AGACAGATTC TGGGTGGCAC CTCTGTCACA 
GAGCGGGATG TGGCTCAAGT GGCCAATGCC ATCCTGTCTA TCAGGAGGAC TCTTCAGGGC 
CTGCCCCTGG ACCTACCCTT AGAGGAGGAG ATGGAATCCT TCTCTGGAGA CCTGGGGAAT 
CTAGACAGAA GCTTCAATCG CCTCCTCCTT ATGTACCGGA GCAAGAAGGA GCAGTTTGAG 
AAGCTAAGCA GTGTGGACCC TTCAGGAGCC TTCCGCATGC TGACTATGGC TTATGAGCAG 
TCCTCCCGGG CTGCACAGCA AGTATCCGAC AGTTCCAGCC TGCTGAGCCA GCTGAGGGAC 
AGTCGCAGAG AGGTAGAAGG TCTGGAGAGA CAGACTGGAG AGGGAGGAGC TGGAGGAGCT 
CAGCTCATGG CCCTGCGCCT AGAGATGGCT TCACTGCCTG ACTTGACACC CACCATCAAC 
AAGCTCTGTG GTGGCTCTAG GCAGACAGCC TGCACCCCAG GAGACTGCCC TGGATTGCTG 
TGTCCCCAGG ACAATGGTAC AGCCTGTGGC TCTCACTGCA GGGGAGCCCT GCCCAGAGCC 
AGAGGGGCCT TACACATGGC AGGGCAGGTA GCTGAGCAGC TACGAAGCTT CAATACCCAG 
CTCCAGCAGA CCAGGCAAAT GATCAGGGCA GCTGAGGAGG CAGCATCAAA GGTCCAATCA 
GATGCCCAGC GCCTTGAGAC TCAGGTGAGT ACCAGCCGCT TACAGATGGA GGAAGATGTC 
CGGCGCACAC GGCTTCTCAT CCAGCAGGTC CGGGGCTTCC TCACAGACCC TCACACAGAT 
GCAGCCACCA TCCAACAGGT GAGCGAGGCA GTGCTGGCTC TCTGGCTGCC CACAGACTCA 
GCTACAGTGC TACGCAAGAT GAAAGAGATC CAGGACATTG CTGCCAGGCT CCCTAACGTG 
GACTTAGTGC TATCCCAGAC TAAGCAGGAC ATTGCACGGG CCCGCAGGCT CCAGGCTGAG 
GCCGAGAAGG CCAGAAGCCG AGCCCACGTT GTGGAAGGGC AGGTGGATGA CGTGGTCGGG 
AACCTCAGGC AGGGCACCGT GGCTCTGCAG GAGGCTCAGG ACACTATGCA GGGCACTGGC 
CGCTCTCTTC GGCTCATCCA GGAAAGGGTT ACTGAGGTTC AGCAGGTCCT GGTACCAGCT 
GAAAGGCTGG TGAAAGGCAT GAAGGAACAG ATGAGTGGAT TCTGGGCACG GGTGAAGGAG 
CTCCGCCATC AGGCCCAGGA AGAGCAGGCA CAGGCAATGA AGGCCCAGCA GCTTGCAGAG 
GGTGCCAGCA ATCAGGCCAT GAATGCTCAG GAGGGCTTTG AGAGACTTAA GCAAAGGTAT 
ACAGAGTTGA AAGACCGGCT GGGTCAGAGT TCTATGCTGG GTGAGCAAGG CAATCGGATC 
TTGAGCATCA AGGCGGAGGC AGAGGAGCTC TTTGGGGAAA CCATGGAAAT GATGGATAAA 
ATGAAAGACA TGGAGTCAGA GCTGCTTCGA GGGAGTCAGG CCATCATGCT TCGTTCAGCA 
GACCTGTCGG GGCTGGAGAA GCGCGTGGAG CAGATCCGCA GTTACATCAA CGGGCGTGTG 
CTGTACTATG CCACCTGCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017454328.1
CDS710..4093
Translation

Target ORF information:

RefSeq Version XM_017598839.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus laminin subunit beta 3 (Lamb3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017598839.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGTGTGGTA AGAGAAATCC AGGTGGCAAT GCCCAGAATG GAAAATGGCT GTGGCAGATG 
AAATGCTGCA AGTGTGACTC CAGGCTGCCT CGCAACTACA ACAGTCACCG AGTGGAGAAC 
GTTGTATCGT CTTCAGGCCC CATGCGCTGG TGGCAGTCCC AGAACGATGT GAGCCCTGTC 
TCTCTTCAAC TGGACCTAGA CAAGAGGATG CAGCTTCAGG ACATAATGAT GGATTTTAAG 
GGTCTCATGC CAGCTGGCAT GTTGATTGAG CGCTCTTCTG ACTTTGGCAA GACATGGAGG 
GTGTACCAGT ACCTGGCGAC AGACTGTGCC AGCACCTTTC CCCAGGTCCA CCAGGGCCAG 
CCCAAGAACT GGCAGGACGT CCGGTGCCGG CCCTTGTCCC AGAGGCCTAA TGGGCATCTG 
ACTGGGGGAA AGGTCCAACT TAACCTTATG GATTTGGCAT CAGCTATCCC TGCATCTCAA 
AGTAAAAAGA TTCAAGAACT AGGAGACATC ACGAACTTGA GAGTCAACTT CACCAAGCTG 
GCCCCAGTGC CCCAGAGGGG CTCCTATCCA CCCAGCGCCT ACTTTGCAGT ATCACAGCTA 
CGTCTACAGG GGAGTTGCTT CTGTCATGGA CATGCCGACC GCTGTGCCCC TAATCCTGGA 
GGCCCTACAG GTTCCACCAC CGCTGTGCAG GTCAACGATG TCTGTGTCTG CCAGCACAAC 
ACAGCTGGCC CCAACTGTGA CCGCTGCGCC CCCTTCTACA ACAACCGGCC ATGGAGACCT 
GCAGAGGGAC AGGACATCCA TGAGTGCCAA AGGTGTGACT GCAATGGGCA CTCAGAGACC 
TGCCACTTCG ACCCAGCTGT GTTTGCTGCT AGCCAAGGGA CAAGTGGAGG CGTGTGTGAC 
AACTGCCAGG ACCACACAGA GGGCAAGAAC TGTGAGCGAT GTCAGCTACA CTATTTCCGG 
AATAGACGAC CAGGCGCTCC CATTCATGAG ACCTGTATTC CCTGCGAGTG TGATCCAGAC 
GGGGCAGTCC CAGGAGCTCC CTGTGACCGA CTGACGGGCC AGTGCGTGTG CAAAGACCAC 
GTGCAAGGGG AGCGCTGTGA TCTTTGCAAG CCTGGTTTCA CAGGGCTCAC CTTCGCCAAC 
CCACAGGGCT GCCATCCCTG TGACTGCAGC ATCTTAGGTA CCCGGCAAGA TATGCCTTGT 
GAGGAGGAAA CTGGACGCTG CTTGTGTCTT CCCAATGTAG TGGGCCCCAA ATGTGACCAG 
TGCGCCCCTT CCCACTGGAA GCTGGCCAGC GGCCGGGGCT GTGAGCCCTG TGCCTGTGAC 
CCACGCAACT CCCTCAGCTC CCAGTGCAAC CAGTTTACAG GGCAGTGTCC TTGTCGGGAA 
GGGTTTGGTG GCCTCACATG TAGTTCTGCA GCCATTCGTC AGTGTCCCGA CCGGACCCAT 
GGAGACGTGG CCACAGGGTG CCGAGCCTGT GACTGTGACT TCAGAGGAAC CGAGGGTCCT 
GGCTGTGACA AAACCTCAGG CCGTTGCCTC TGCCGCCCCG GCTTGACAGG GCCCCGCTGC 
GACCAGTGCC AACGAGGCTA CTGCGACCGC TACCCAGTGT GTGTGGCCTG CCACTCTTGC 
TTCCAGGCCT ATGACACAGA CCTCCAGGAG CAGGCTTGGC GCCTTGGCAG TCTCCGTAAT 
ACCACTGAAG GCCTGGGCAC AGGGACAGGT CTGGAGGACC GTGGCCTGGC TTCTCGGTTG 
CTAGATGCCA AGAGCAAAAT CGAGCAGATC AGACAGATTC TGGGTGGCAC CTCTGTCACA 
GAGCGGGATG TGGCTCAAGT GGCCAATGCC ATCCTGTCTA TCAGGAGGAC TCTTCAGGGC 
CTGCCCCTGG ACCTACCCTT AGAGGAGGAG ATGGAATCCT TCTCTGGAGA CCTGGGGAAT 
CTAGACAGAA GCTTCAATCG CCTCCTCCTT ATGTACCGGA GCAAGAAGGA GCAGTTTGAG 
AAGCTAAGCA GTGTGGACCC TTCAGGAGCC TTCCGCATGC TGACTATGGC TTATGAGCAG 
TCCTCCCGGG CTGCACAGCA AGTATCCGAC AGTTCCAGCC TGCTGAGCCA GCTGAGGGAC 
AGTCGCAGAG AGGTAGAAGG TCTGGAGAGA CAGACTGGAG AGGGAGGAGC TGGAGGAGCT 
CAGCTCATGG CCCTGCGCCT AGAGATGGCT TCACTGCCTG ACTTGACACC CACCATCAAC 
AAGCTCTGTG GTGGCTCTAG GCAGACAGCC TGCACCCCAG GAGACTGCCC TGGATTGCTG 
TGTCCCCAGG ACAATGGTAC AGCCTGTGGC TCTCACTGCA GGGGAGCCCT GCCCAGAGCC 
AGAGGGGCCT TACACATGGC AGGGCAGGTA GCTGAGCAGC TACGAAGCTT CAATACCCAG 
CTCCAGCAGA CCAGGCAAAT GATCAGGGCA GCTGAGGAGG CAGCATCAAA GGTCCAATCA 
GATGCCCAGC GCCTTGAGAC TCAGGTGAGT ACCAGCCGCT TACAGATGGA GGAAGATGTC 
CGGCGCACAC GGCTTCTCAT CCAGCAGGTC CGGGGCTTCC TCACAGACCC TCACACAGAT 
GCAGCCACCA TCCAACAGGT GAGCGAGGCA GTGCTGGCTC TCTGGCTGCC CACAGACTCA 
GCTACAGTGC TACGCAAGAT GAAAGAGATC CAGGACATTG CTGCCAGGCT CCCTAACGTG 
GACTTAGTGC TATCCCAGAC TAAGCAGGAC ATTGCACGGG CCCGCAGGCT CCAGGCTGAG 
GCCGAGAAGG CCAGAAGCCG AGCCCACGTT GTGGAAGGGC AGGTGGATGA CGTGGTCGGG 
AACCTCAGGC AGGGCACCGT GGCTCTGCAG GAGGCTCAGG ACACTATGCA GGGCACTGGC 
CGCTCTCTTC GGCTCATCCA GGAAAGGGTT ACTGAGGTTC AGCAGGTCCT GGTACCAGCT 
GAAAGGCTGG TGAAAGGCAT GAAGGAACAG ATGAGTGGAT TCTGGGCACG GGTGAAGGAG 
CTCCGCCATC AGGCCCAGGA AGAGCAGGCA CAGGCAATGA AGGCCCAGCA GCTTGCAGAG 
GGTGCCAGCA ATCAGGCCAT GAATGCTCAG GAGGGCTTTG AGAGACTTAA GCAAAGGTAT 
ACAGAGTTGA AAGACCGGCT GGGTCAGAGT TCTATGCTGG GTGAGCAAGG CAATCGGATC 
TTGAGCATCA AGGCGGAGGC AGAGGAGCTC TTTGGGGAAA CCATGGAAAT GATGGATAAA 
ATGAAAGACA TGGAGTCAGA GCTGCTTCGA GGGAGTCAGG CCATCATGCT TCGTTCAGCA 
GACCTGTCGG GGCTGGAGAA GCGCGTGGAG CAGATCCGCA GTTACATCAA CGGGCGTGTG 
CTGTACTATG CCACCTGCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa11134
Clone ID Related Accession (Same CDS sequence) XM_008769856.2 , NM_001100841.1
Accession Version NM_001100841.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-12
Organism Rattus norvegicus(Norway rat)
Product laminin subunit beta-3 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126166.4. On or before Dec 19, 2009 this sequence version replaced XM_223087.4, XM_001069930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGATGACGG CACTTTTCCT CTTGTGGCTT GCCTTGCCTG GCCTCCTGTG TGCCCAGCAA 
CCCTGCTCCC GAGGGGCCTG TTATCCACCT GTTGGGGACT TGCTCATTGG AAGGACTCAG 
CTTCTTCGAG CCTCGTCTAC CTGTGGATTG ACCAAGCCTG AGACCTATTG CACCCAATAT 
GGACAGTGGC AGATGAAATG CTGCAAGTGT GACTCCAGGC TGCCTCGCAA CTACAACAGT 
CACCGAGTGG AGAACGTTGT ATCGTCTTCA GGCCCCATGC GCTGGTGGCA GTCCCAGAAC 
GATGTGAGCC CTGTCTCTCT TCAACTGGAC CTAGACAAGA GGATGCAGCT TCAGGACATA 
ATGATGGATT TTAAGGGTCT CATGCCAGCT GGCATGTTGA TTGAGCGCTC TTCTGACTTT 
GGCAAGACAT GGAGGGTGTA CCAGTACCTG GCGACAGACT GTGCCAGCAC CTTTCCCCAG 
GTCCACCAGG GCCAGCCCAA GAACTGGCAG GACGTCCGGT GCCGGCCCTT GTCCCAGAGG 
CCTAATGGGC ATCTGACTGG GGGAAAGGTC CAACTTAACC TTATGGATTT GGCATCAGCT 
ATCCCTGCAT CTCAAAGTAA AAAGATTCAA GAACTAGGAG ACATCACGAA CTTGAGAGTC 
AACTTCACCA AGCTGGCCCC AGTGCCCCAG AGGGGCTCCT ATCCACCCAG CGCCTACTTT 
GCAGTATCAC AGCTACGTCT ACAGGGGAGT TGCTTCTGTC ATGGACATGC CGACCGCTGT 
GCCCCTAATC CTGGAGGCCC TACAGGTTCC ACCACCGCTG TGCAGGTCAA CGATGTCTGT 
GTCTGCCAGC ACAACACAGC TGGCCCCAAC TGTGACCGCT GCGCCCCCTT CTACAACAAC 
CGGCCATGGA GACCTGCAGA GGGACAGGAC ATCCATGAGT GCCAAAGGTG TGACTGCAAT 
GGGCACTCAG AGACCTGCCA CTTCGACCCA GCTGTGTTTG CTGCTAGCCA AGGGACAAGT 
GGAGGCGTGT GTGACAACTG CCAGGACCAC ACAGAGGGCA AGAACTGTGA GCGATGTCAG 
CTACACTATT TCCGGAATAG ACGACCAGGC GCTCCCATTC ATGAGACCTG TATTCCCTGC 
GAGTGTGATC CAGACGGGGC AGTCCCAGGA GCTCCCTGTG ACCGACTGAC GGGCCAGTGC 
GTGTGCAAAG ACCACGTGCA AGGGGAGCGC TGTGATCTTT GCAAGCCTGG TTTCACAGGG 
CTCACCTTCG CCAACCCACA GGGCTGCCAT CCCTGTGACT GCAGCATCTT AGGTACCCGG 
CAAGATATGC CTTGTGAGGA GGAAACTGGA CGCTGCTTGT GTCTTCCCAA TGTAGTGGGC 
CCCAAATGTG ACCAGTGCGC CCCTTCCCAC TGGAAGCTGG CCAGCGGCCG GGGCTGTGAG 
CCCTGTGCCT GTGACCCACG CAACTCCCTC AGCTCCCAGT GCAACCAGTT TACAGGGCAG 
TGTCCTTGTC GGGAAGGGTT TGGTGGCCTC ACATGTAGTT CTGCAGCCAT TCGTCAGTGT 
CCCGACCGGA CCCATGGAGA CGTGGCCACA GGGTGCCGAG CCTGTGACTG TGACTTCAGA 
GGAACCGAGG GTCCTGGCTG TGACAAAACC TCAGGCCGTT GCCTCTGCCG CCCCGGCTTG 
ACAGGGCCCC GCTGCGACCA GTGCCAACGA GGCTACTGCG ACCGCTACCC AGTGTGTGTG 
GCCTGCCACT CTTGCTTCCA GGCCTATGAC ACAGACCTCC AGGAGCAGGC TTGGCGCCTT 
GGCAGTCTCC GTAATACCAC TGAAGGCCTG GGCACAGGGA CAGGTCTGGA GGACCGTGGC 
CTGGCTTCTC GGTTGCTAGA TGCCAAGAGC AAAATCGAGC AGATCAGACA GATTCTGGGT 
GGCACCTCTG TCACAGAGCG GGATGTGGCT CAAGTGGCCA ATGCCATCCT GTCTATCAGG 
AGGACTCTTC AGGGCCTGCC CCTGGACCTA CCCTTAGAGG AGGAGATGGA ATCCTTCTCT 
GGAGACCTGG GGAATCTAGA CAGAAGCTTC AATCGCCTCC TCCTTATGTA CCGGAGCAAG 
AAGGAGCAGT TTGAGAAGCT AAGCAGTGTG GACCCTTCAG GAGCCTTCCG CATGCTGACT 
ATGGCTTATG AGCAGTCCTC CCGGGCTGCA CAGCAAGTAT CCGACAGTTC CAGCCTGCTG 
AGCCAGCTGA GGGACAGTCG CAGAGAGGTA GAAGGTCTGG AGAGACAGAC TGGAGAGGGA 
GGAGCTGGAG GAGCTCAGCT CATGGCCCTG CGCCTAGAGA TGGCTTCACT GCCTGACTTG 
ACACCCACCA TCAACAAGCT CTGTGGTGGC TCTAGGCAGA CAGCCTGCAC CCCAGGAGAC 
TGCCCTGGAT TGCTGTGTCC CCAGGACAAT GGTACAGCCT GTGGCTCTCA CTGCAGGGGA 
GCCCTGCCCA GAGCCAGAGG GGCCTTACAC ATGGCAGGGC AGGTAGCTGA GCAGCTACGA 
AGCTTCAATA CCCAGCTCCA GCAGACCAGG CAAATGATCA GGGCAGCTGA GGAGGCAGCA 
TCAAAGGTCC AATCAGATGC CCAGCGCCTT GAGACTCAGG TGAGTACCAG CCGCTTACAG 
ATGGAGGAAG ATGTCCGGCG CACACGGCTT CTCATCCAGC AGGTCCGGGG CTTCCTCACA 
GACCCTCACA CAGATGCAGC CACCATCCAA CAGGTGAGCG AGGCAGTGCT GGCTCTCTGG 
CTGCCCACAG ACTCAGCTAC AGTGCTACGC AAGATGAAAG AGATCCAGGA CATTGCTGCC 
AGGCTCCCTA ACGTGGACTT AGTGCTATCC CAGACTAAGC AGGACATTGC ACGGGCCCGC 
AGGCTCCAGG CTGAGGCCGA GAAGGCCAGA AGCCGAGCCC ACGTTGTGGA AGGGCAGGTG 
GATGACGTGG TCGGGAACCT CAGGCAGGGC ACCGTGGCTC TGCAGGAGGC TCAGGACACT 
ATGCAGGGCA CTGGCCGCTC TCTTCGGCTC ATCCAGGAAA GGGTTACTGA GGTTCAGCAG 
GTCCTGGTAC CAGCTGAAAG GCTGGTGAAA GGCATGAAGG AACAGATGAG TGGATTCTGG 
GCACGGGTGA AGGAGCTCCG CCATCAGGCC CAGGAAGAGC AGGCACAGGC AATGAAGGCC 
CAGCAGCTTG CAGAGGGTGC CAGCAATCAG GCCATGAATG CTCAGGAGGG CTTTGAGAGA 
CTTAAGCAAA GGTATACAGA GTTGAAAGAC CGGCTGGGTC AGAGTTCTAT GCTGGGTGAG 
CAAGGCAATC GGATCTTGAG CATCAAGGCG GAGGCAGAGG AGCTCTTTGG GGAAACCATG 
GAAATGATGG ATAAAATGAA AGACATGGAG TCAGAGCTGC TTCGAGGGAG TCAGGCCATC 
ATGCTTCGTT CAGCAGACCT GTCGGGGCTG GAGAAGCGCG TGGAGCAGAT CCGCAGTTAC 
ATCAACGGGC GTGTGCTGTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001094311.1
CDS178..3696
Translation

Target ORF information:

RefSeq Version NM_001100841.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus laminin subunit beta 3 (Lamb3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001100841.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGATGACGG CACTTTTCCT CTTGTGGCTT GCCTTGCCTG GCCTCCTGTG TGCCCAGCAA 
CCCTGCTCCC GAGGGGCCTG TTATCCACCT GTTGGGGACT TGCTCATTGG AAGGACTCAG 
CTTCTTCGAG CCTCGTCTAC CTGTGGATTG ACCAAGCCTG AGACCTATTG CACCCAATAT 
GGACAGTGGC AGATGAAATG CTGCAAGTGT GACTCCAGGC TGCCTCGCAA CTACAACAGT 
CACCGAGTGG AGAACGTTGT ATCGTCTTCA GGCCCCATGC GCTGGTGGCA GTCCCAGAAC 
GATGTGAGCC CTGTCTCTCT TCAACTGGAC CTAGACAAGA GGATGCAGCT TCAGGACATA 
ATGATGGATT TTAAGGGTCT CATGCCAGCT GGCATGTTGA TTGAGCGCTC TTCTGACTTT 
GGCAAGACAT GGAGGGTGTA CCAGTACCTG GCGACAGACT GTGCCAGCAC CTTTCCCCAG 
GTCCACCAGG GCCAGCCCAA GAACTGGCAG GACGTCCGGT GCCGGCCCTT GTCCCAGAGG 
CCTAATGGGC ATCTGACTGG GGGAAAGGTC CAACTTAACC TTATGGATTT GGCATCAGCT 
ATCCCTGCAT CTCAAAGTAA AAAGATTCAA GAACTAGGAG ACATCACGAA CTTGAGAGTC 
AACTTCACCA AGCTGGCCCC AGTGCCCCAG AGGGGCTCCT ATCCACCCAG CGCCTACTTT 
GCAGTATCAC AGCTACGTCT ACAGGGGAGT TGCTTCTGTC ATGGACATGC CGACCGCTGT 
GCCCCTAATC CTGGAGGCCC TACAGGTTCC ACCACCGCTG TGCAGGTCAA CGATGTCTGT 
GTCTGCCAGC ACAACACAGC TGGCCCCAAC TGTGACCGCT GCGCCCCCTT CTACAACAAC 
CGGCCATGGA GACCTGCAGA GGGACAGGAC ATCCATGAGT GCCAAAGGTG TGACTGCAAT 
GGGCACTCAG AGACCTGCCA CTTCGACCCA GCTGTGTTTG CTGCTAGCCA AGGGACAAGT 
GGAGGCGTGT GTGACAACTG CCAGGACCAC ACAGAGGGCA AGAACTGTGA GCGATGTCAG 
CTACACTATT TCCGGAATAG ACGACCAGGC GCTCCCATTC ATGAGACCTG TATTCCCTGC 
GAGTGTGATC CAGACGGGGC AGTCCCAGGA GCTCCCTGTG ACCGACTGAC GGGCCAGTGC 
GTGTGCAAAG ACCACGTGCA AGGGGAGCGC TGTGATCTTT GCAAGCCTGG TTTCACAGGG 
CTCACCTTCG CCAACCCACA GGGCTGCCAT CCCTGTGACT GCAGCATCTT AGGTACCCGG 
CAAGATATGC CTTGTGAGGA GGAAACTGGA CGCTGCTTGT GTCTTCCCAA TGTAGTGGGC 
CCCAAATGTG ACCAGTGCGC CCCTTCCCAC TGGAAGCTGG CCAGCGGCCG GGGCTGTGAG 
CCCTGTGCCT GTGACCCACG CAACTCCCTC AGCTCCCAGT GCAACCAGTT TACAGGGCAG 
TGTCCTTGTC GGGAAGGGTT TGGTGGCCTC ACATGTAGTT CTGCAGCCAT TCGTCAGTGT 
CCCGACCGGA CCCATGGAGA CGTGGCCACA GGGTGCCGAG CCTGTGACTG TGACTTCAGA 
GGAACCGAGG GTCCTGGCTG TGACAAAACC TCAGGCCGTT GCCTCTGCCG CCCCGGCTTG 
ACAGGGCCCC GCTGCGACCA GTGCCAACGA GGCTACTGCG ACCGCTACCC AGTGTGTGTG 
GCCTGCCACT CTTGCTTCCA GGCCTATGAC ACAGACCTCC AGGAGCAGGC TTGGCGCCTT 
GGCAGTCTCC GTAATACCAC TGAAGGCCTG GGCACAGGGA CAGGTCTGGA GGACCGTGGC 
CTGGCTTCTC GGTTGCTAGA TGCCAAGAGC AAAATCGAGC AGATCAGACA GATTCTGGGT 
GGCACCTCTG TCACAGAGCG GGATGTGGCT CAAGTGGCCA ATGCCATCCT GTCTATCAGG 
AGGACTCTTC AGGGCCTGCC CCTGGACCTA CCCTTAGAGG AGGAGATGGA ATCCTTCTCT 
GGAGACCTGG GGAATCTAGA CAGAAGCTTC AATCGCCTCC TCCTTATGTA CCGGAGCAAG 
AAGGAGCAGT TTGAGAAGCT AAGCAGTGTG GACCCTTCAG GAGCCTTCCG CATGCTGACT 
ATGGCTTATG AGCAGTCCTC CCGGGCTGCA CAGCAAGTAT CCGACAGTTC CAGCCTGCTG 
AGCCAGCTGA GGGACAGTCG CAGAGAGGTA GAAGGTCTGG AGAGACAGAC TGGAGAGGGA 
GGAGCTGGAG GAGCTCAGCT CATGGCCCTG CGCCTAGAGA TGGCTTCACT GCCTGACTTG 
ACACCCACCA TCAACAAGCT CTGTGGTGGC TCTAGGCAGA CAGCCTGCAC CCCAGGAGAC 
TGCCCTGGAT TGCTGTGTCC CCAGGACAAT GGTACAGCCT GTGGCTCTCA CTGCAGGGGA 
GCCCTGCCCA GAGCCAGAGG GGCCTTACAC ATGGCAGGGC AGGTAGCTGA GCAGCTACGA 
AGCTTCAATA CCCAGCTCCA GCAGACCAGG CAAATGATCA GGGCAGCTGA GGAGGCAGCA 
TCAAAGGTCC AATCAGATGC CCAGCGCCTT GAGACTCAGG TGAGTACCAG CCGCTTACAG 
ATGGAGGAAG ATGTCCGGCG CACACGGCTT CTCATCCAGC AGGTCCGGGG CTTCCTCACA 
GACCCTCACA CAGATGCAGC CACCATCCAA CAGGTGAGCG AGGCAGTGCT GGCTCTCTGG 
CTGCCCACAG ACTCAGCTAC AGTGCTACGC AAGATGAAAG AGATCCAGGA CATTGCTGCC 
AGGCTCCCTA ACGTGGACTT AGTGCTATCC CAGACTAAGC AGGACATTGC ACGGGCCCGC 
AGGCTCCAGG CTGAGGCCGA GAAGGCCAGA AGCCGAGCCC ACGTTGTGGA AGGGCAGGTG 
GATGACGTGG TCGGGAACCT CAGGCAGGGC ACCGTGGCTC TGCAGGAGGC TCAGGACACT 
ATGCAGGGCA CTGGCCGCTC TCTTCGGCTC ATCCAGGAAA GGGTTACTGA GGTTCAGCAG 
GTCCTGGTAC CAGCTGAAAG GCTGGTGAAA GGCATGAAGG AACAGATGAG TGGATTCTGG 
GCACGGGTGA AGGAGCTCCG CCATCAGGCC CAGGAAGAGC AGGCACAGGC AATGAAGGCC 
CAGCAGCTTG CAGAGGGTGC CAGCAATCAG GCCATGAATG CTCAGGAGGG CTTTGAGAGA 
CTTAAGCAAA GGTATACAGA GTTGAAAGAC CGGCTGGGTC AGAGTTCTAT GCTGGGTGAG 
CAAGGCAATC GGATCTTGAG CATCAAGGCG GAGGCAGAGG AGCTCTTTGG GGAAACCATG 
GAAATGATGG ATAAAATGAA AGACATGGAG TCAGAGCTGC TTCGAGGGAG TCAGGCCATC 
ATGCTTCGTT CAGCAGACCT GTCGGGGCTG GAGAAGCGCG TGGAGCAGAT CCGCAGTTAC 
ATCAACGGGC GTGTGCTGTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa11134
Clone ID Related Accession (Same CDS sequence) XM_008769856.2 , NM_001100841.1
Accession Version XM_008769856.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGATGACGG CACTTTTCCT CTTGTGGCTT GCCTTGCCTG GCCTCCTGTG TGCCCAGCAA 
CCCTGCTCCC GAGGGGCCTG TTATCCACCT GTTGGGGACT TGCTCATTGG AAGGACTCAG 
CTTCTTCGAG CCTCGTCTAC CTGTGGATTG ACCAAGCCTG AGACCTATTG CACCCAATAT 
GGACAGTGGC AGATGAAATG CTGCAAGTGT GACTCCAGGC TGCCTCGCAA CTACAACAGT 
CACCGAGTGG AGAACGTTGT ATCGTCTTCA GGCCCCATGC GCTGGTGGCA GTCCCAGAAC 
GATGTGAGCC CTGTCTCTCT TCAACTGGAC CTAGACAAGA GGATGCAGCT TCAGGACATA 
ATGATGGATT TTAAGGGTCT CATGCCAGCT GGCATGTTGA TTGAGCGCTC TTCTGACTTT 
GGCAAGACAT GGAGGGTGTA CCAGTACCTG GCGACAGACT GTGCCAGCAC CTTTCCCCAG 
GTCCACCAGG GCCAGCCCAA GAACTGGCAG GACGTCCGGT GCCGGCCCTT GTCCCAGAGG 
CCTAATGGGC ATCTGACTGG GGGAAAGGTC CAACTTAACC TTATGGATTT GGCATCAGCT 
ATCCCTGCAT CTCAAAGTAA AAAGATTCAA GAACTAGGAG ACATCACGAA CTTGAGAGTC 
AACTTCACCA AGCTGGCCCC AGTGCCCCAG AGGGGCTCCT ATCCACCCAG CGCCTACTTT 
GCAGTATCAC AGCTACGTCT ACAGGGGAGT TGCTTCTGTC ATGGACATGC CGACCGCTGT 
GCCCCTAATC CTGGAGGCCC TACAGGTTCC ACCACCGCTG TGCAGGTCAA CGATGTCTGT 
GTCTGCCAGC ACAACACAGC TGGCCCCAAC TGTGACCGCT GCGCCCCCTT CTACAACAAC 
CGGCCATGGA GACCTGCAGA GGGACAGGAC ATCCATGAGT GCCAAAGGTG TGACTGCAAT 
GGGCACTCAG AGACCTGCCA CTTCGACCCA GCTGTGTTTG CTGCTAGCCA AGGGACAAGT 
GGAGGCGTGT GTGACAACTG CCAGGACCAC ACAGAGGGCA AGAACTGTGA GCGATGTCAG 
CTACACTATT TCCGGAATAG ACGACCAGGC GCTCCCATTC ATGAGACCTG TATTCCCTGC 
GAGTGTGATC CAGACGGGGC AGTCCCAGGA GCTCCCTGTG ACCGACTGAC GGGCCAGTGC 
GTGTGCAAAG ACCACGTGCA AGGGGAGCGC TGTGATCTTT GCAAGCCTGG TTTCACAGGG 
CTCACCTTCG CCAACCCACA GGGCTGCCAT CCCTGTGACT GCAGCATCTT AGGTACCCGG 
CAAGATATGC CTTGTGAGGA GGAAACTGGA CGCTGCTTGT GTCTTCCCAA TGTAGTGGGC 
CCCAAATGTG ACCAGTGCGC CCCTTCCCAC TGGAAGCTGG CCAGCGGCCG GGGCTGTGAG 
CCCTGTGCCT GTGACCCACG CAACTCCCTC AGCTCCCAGT GCAACCAGTT TACAGGGCAG 
TGTCCTTGTC GGGAAGGGTT TGGTGGCCTC ACATGTAGTT CTGCAGCCAT TCGTCAGTGT 
CCCGACCGGA CCCATGGAGA CGTGGCCACA GGGTGCCGAG CCTGTGACTG TGACTTCAGA 
GGAACCGAGG GTCCTGGCTG TGACAAAACC TCAGGCCGTT GCCTCTGCCG CCCCGGCTTG 
ACAGGGCCCC GCTGCGACCA GTGCCAACGA GGCTACTGCG ACCGCTACCC AGTGTGTGTG 
GCCTGCCACT CTTGCTTCCA GGCCTATGAC ACAGACCTCC AGGAGCAGGC TTGGCGCCTT 
GGCAGTCTCC GTAATACCAC TGAAGGCCTG GGCACAGGGA CAGGTCTGGA GGACCGTGGC 
CTGGCTTCTC GGTTGCTAGA TGCCAAGAGC AAAATCGAGC AGATCAGACA GATTCTGGGT 
GGCACCTCTG TCACAGAGCG GGATGTGGCT CAAGTGGCCA ATGCCATCCT GTCTATCAGG 
AGGACTCTTC AGGGCCTGCC CCTGGACCTA CCCTTAGAGG AGGAGATGGA ATCCTTCTCT 
GGAGACCTGG GGAATCTAGA CAGAAGCTTC AATCGCCTCC TCCTTATGTA CCGGAGCAAG 
AAGGAGCAGT TTGAGAAGCT AAGCAGTGTG GACCCTTCAG GAGCCTTCCG CATGCTGACT 
ATGGCTTATG AGCAGTCCTC CCGGGCTGCA CAGCAAGTAT CCGACAGTTC CAGCCTGCTG 
AGCCAGCTGA GGGACAGTCG CAGAGAGGTA GAAGGTCTGG AGAGACAGAC TGGAGAGGGA 
GGAGCTGGAG GAGCTCAGCT CATGGCCCTG CGCCTAGAGA TGGCTTCACT GCCTGACTTG 
ACACCCACCA TCAACAAGCT CTGTGGTGGC TCTAGGCAGA CAGCCTGCAC CCCAGGAGAC 
TGCCCTGGAT TGCTGTGTCC CCAGGACAAT GGTACAGCCT GTGGCTCTCA CTGCAGGGGA 
GCCCTGCCCA GAGCCAGAGG GGCCTTACAC ATGGCAGGGC AGGTAGCTGA GCAGCTACGA 
AGCTTCAATA CCCAGCTCCA GCAGACCAGG CAAATGATCA GGGCAGCTGA GGAGGCAGCA 
TCAAAGGTCC AATCAGATGC CCAGCGCCTT GAGACTCAGG TGAGTACCAG CCGCTTACAG 
ATGGAGGAAG ATGTCCGGCG CACACGGCTT CTCATCCAGC AGGTCCGGGG CTTCCTCACA 
GACCCTCACA CAGATGCAGC CACCATCCAA CAGGTGAGCG AGGCAGTGCT GGCTCTCTGG 
CTGCCCACAG ACTCAGCTAC AGTGCTACGC AAGATGAAAG AGATCCAGGA CATTGCTGCC 
AGGCTCCCTA ACGTGGACTT AGTGCTATCC CAGACTAAGC AGGACATTGC ACGGGCCCGC 
AGGCTCCAGG CTGAGGCCGA GAAGGCCAGA AGCCGAGCCC ACGTTGTGGA AGGGCAGGTG 
GATGACGTGG TCGGGAACCT CAGGCAGGGC ACCGTGGCTC TGCAGGAGGC TCAGGACACT 
ATGCAGGGCA CTGGCCGCTC TCTTCGGCTC ATCCAGGAAA GGGTTACTGA GGTTCAGCAG 
GTCCTGGTAC CAGCTGAAAG GCTGGTGAAA GGCATGAAGG AACAGATGAG TGGATTCTGG 
GCACGGGTGA AGGAGCTCCG CCATCAGGCC CAGGAAGAGC AGGCACAGGC AATGAAGGCC 
CAGCAGCTTG CAGAGGGTGC CAGCAATCAG GCCATGAATG CTCAGGAGGG CTTTGAGAGA 
CTTAAGCAAA GGTATACAGA GTTGAAAGAC CGGCTGGGTC AGAGTTCTAT GCTGGGTGAG 
CAAGGCAATC GGATCTTGAG CATCAAGGCG GAGGCAGAGG AGCTCTTTGG GGAAACCATG 
GAAATGATGG ATAAAATGAA AGACATGGAG TCAGAGCTGC TTCGAGGGAG TCAGGCCATC 
ATGCTTCGTT CAGCAGACCT GTCGGGGCTG GAGAAGCGCG TGGAGCAGAT CCGCAGTTAC 
ATCAACGGGC GTGTGCTGTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008768078.1
CDS110..3628
Translation

Target ORF information:

RefSeq Version XM_008769856.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus laminin subunit beta 3 (Lamb3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008769856.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGATGACGG CACTTTTCCT CTTGTGGCTT GCCTTGCCTG GCCTCCTGTG TGCCCAGCAA 
CCCTGCTCCC GAGGGGCCTG TTATCCACCT GTTGGGGACT TGCTCATTGG AAGGACTCAG 
CTTCTTCGAG CCTCGTCTAC CTGTGGATTG ACCAAGCCTG AGACCTATTG CACCCAATAT 
GGACAGTGGC AGATGAAATG CTGCAAGTGT GACTCCAGGC TGCCTCGCAA CTACAACAGT 
CACCGAGTGG AGAACGTTGT ATCGTCTTCA GGCCCCATGC GCTGGTGGCA GTCCCAGAAC 
GATGTGAGCC CTGTCTCTCT TCAACTGGAC CTAGACAAGA GGATGCAGCT TCAGGACATA 
ATGATGGATT TTAAGGGTCT CATGCCAGCT GGCATGTTGA TTGAGCGCTC TTCTGACTTT 
GGCAAGACAT GGAGGGTGTA CCAGTACCTG GCGACAGACT GTGCCAGCAC CTTTCCCCAG 
GTCCACCAGG GCCAGCCCAA GAACTGGCAG GACGTCCGGT GCCGGCCCTT GTCCCAGAGG 
CCTAATGGGC ATCTGACTGG GGGAAAGGTC CAACTTAACC TTATGGATTT GGCATCAGCT 
ATCCCTGCAT CTCAAAGTAA AAAGATTCAA GAACTAGGAG ACATCACGAA CTTGAGAGTC 
AACTTCACCA AGCTGGCCCC AGTGCCCCAG AGGGGCTCCT ATCCACCCAG CGCCTACTTT 
GCAGTATCAC AGCTACGTCT ACAGGGGAGT TGCTTCTGTC ATGGACATGC CGACCGCTGT 
GCCCCTAATC CTGGAGGCCC TACAGGTTCC ACCACCGCTG TGCAGGTCAA CGATGTCTGT 
GTCTGCCAGC ACAACACAGC TGGCCCCAAC TGTGACCGCT GCGCCCCCTT CTACAACAAC 
CGGCCATGGA GACCTGCAGA GGGACAGGAC ATCCATGAGT GCCAAAGGTG TGACTGCAAT 
GGGCACTCAG AGACCTGCCA CTTCGACCCA GCTGTGTTTG CTGCTAGCCA AGGGACAAGT 
GGAGGCGTGT GTGACAACTG CCAGGACCAC ACAGAGGGCA AGAACTGTGA GCGATGTCAG 
CTACACTATT TCCGGAATAG ACGACCAGGC GCTCCCATTC ATGAGACCTG TATTCCCTGC 
GAGTGTGATC CAGACGGGGC AGTCCCAGGA GCTCCCTGTG ACCGACTGAC GGGCCAGTGC 
GTGTGCAAAG ACCACGTGCA AGGGGAGCGC TGTGATCTTT GCAAGCCTGG TTTCACAGGG 
CTCACCTTCG CCAACCCACA GGGCTGCCAT CCCTGTGACT GCAGCATCTT AGGTACCCGG 
CAAGATATGC CTTGTGAGGA GGAAACTGGA CGCTGCTTGT GTCTTCCCAA TGTAGTGGGC 
CCCAAATGTG ACCAGTGCGC CCCTTCCCAC TGGAAGCTGG CCAGCGGCCG GGGCTGTGAG 
CCCTGTGCCT GTGACCCACG CAACTCCCTC AGCTCCCAGT GCAACCAGTT TACAGGGCAG 
TGTCCTTGTC GGGAAGGGTT TGGTGGCCTC ACATGTAGTT CTGCAGCCAT TCGTCAGTGT 
CCCGACCGGA CCCATGGAGA CGTGGCCACA GGGTGCCGAG CCTGTGACTG TGACTTCAGA 
GGAACCGAGG GTCCTGGCTG TGACAAAACC TCAGGCCGTT GCCTCTGCCG CCCCGGCTTG 
ACAGGGCCCC GCTGCGACCA GTGCCAACGA GGCTACTGCG ACCGCTACCC AGTGTGTGTG 
GCCTGCCACT CTTGCTTCCA GGCCTATGAC ACAGACCTCC AGGAGCAGGC TTGGCGCCTT 
GGCAGTCTCC GTAATACCAC TGAAGGCCTG GGCACAGGGA CAGGTCTGGA GGACCGTGGC 
CTGGCTTCTC GGTTGCTAGA TGCCAAGAGC AAAATCGAGC AGATCAGACA GATTCTGGGT 
GGCACCTCTG TCACAGAGCG GGATGTGGCT CAAGTGGCCA ATGCCATCCT GTCTATCAGG 
AGGACTCTTC AGGGCCTGCC CCTGGACCTA CCCTTAGAGG AGGAGATGGA ATCCTTCTCT 
GGAGACCTGG GGAATCTAGA CAGAAGCTTC AATCGCCTCC TCCTTATGTA CCGGAGCAAG 
AAGGAGCAGT TTGAGAAGCT AAGCAGTGTG GACCCTTCAG GAGCCTTCCG CATGCTGACT 
ATGGCTTATG AGCAGTCCTC CCGGGCTGCA CAGCAAGTAT CCGACAGTTC CAGCCTGCTG 
AGCCAGCTGA GGGACAGTCG CAGAGAGGTA GAAGGTCTGG AGAGACAGAC TGGAGAGGGA 
GGAGCTGGAG GAGCTCAGCT CATGGCCCTG CGCCTAGAGA TGGCTTCACT GCCTGACTTG 
ACACCCACCA TCAACAAGCT CTGTGGTGGC TCTAGGCAGA CAGCCTGCAC CCCAGGAGAC 
TGCCCTGGAT TGCTGTGTCC CCAGGACAAT GGTACAGCCT GTGGCTCTCA CTGCAGGGGA 
GCCCTGCCCA GAGCCAGAGG GGCCTTACAC ATGGCAGGGC AGGTAGCTGA GCAGCTACGA 
AGCTTCAATA CCCAGCTCCA GCAGACCAGG CAAATGATCA GGGCAGCTGA GGAGGCAGCA 
TCAAAGGTCC AATCAGATGC CCAGCGCCTT GAGACTCAGG TGAGTACCAG CCGCTTACAG 
ATGGAGGAAG ATGTCCGGCG CACACGGCTT CTCATCCAGC AGGTCCGGGG CTTCCTCACA 
GACCCTCACA CAGATGCAGC CACCATCCAA CAGGTGAGCG AGGCAGTGCT GGCTCTCTGG 
CTGCCCACAG ACTCAGCTAC AGTGCTACGC AAGATGAAAG AGATCCAGGA CATTGCTGCC 
AGGCTCCCTA ACGTGGACTT AGTGCTATCC CAGACTAAGC AGGACATTGC ACGGGCCCGC 
AGGCTCCAGG CTGAGGCCGA GAAGGCCAGA AGCCGAGCCC ACGTTGTGGA AGGGCAGGTG 
GATGACGTGG TCGGGAACCT CAGGCAGGGC ACCGTGGCTC TGCAGGAGGC TCAGGACACT 
ATGCAGGGCA CTGGCCGCTC TCTTCGGCTC ATCCAGGAAA GGGTTACTGA GGTTCAGCAG 
GTCCTGGTAC CAGCTGAAAG GCTGGTGAAA GGCATGAAGG AACAGATGAG TGGATTCTGG 
GCACGGGTGA AGGAGCTCCG CCATCAGGCC CAGGAAGAGC AGGCACAGGC AATGAAGGCC 
CAGCAGCTTG CAGAGGGTGC CAGCAATCAG GCCATGAATG CTCAGGAGGG CTTTGAGAGA 
CTTAAGCAAA GGTATACAGA GTTGAAAGAC CGGCTGGGTC AGAGTTCTAT GCTGGGTGAG 
CAAGGCAATC GGATCTTGAG CATCAAGGCG GAGGCAGAGG AGCTCTTTGG GGAAACCATG 
GAAATGATGG ATAAAATGAA AGACATGGAG TCAGAGCTGC TTCGAGGGAG TCAGGCCATC 
ATGCTTCGTT CAGCAGACCT GTCGGGGCTG GAGAAGCGCG TGGAGCAGAT CCGCAGTTAC 
ATCAACGGGC GTGTGCTGTA CTATGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K


book

Epitope mapping of function-blocking monoclonal antibody CM6 suggests a "weak" integrin binding site on the laminin-332 LG2 domain.
Journal of cellular physiology223(3)541-8(2010 Jun)
Yamashita H,Shang M,Tripathi M,Jourquin J,Georgescu W,Liu S,Weidow B,Quaranta V


book

Laminin-332-beta1 integrin interactions negatively regulate invadopodia.
Journal of cellular physiology223(1)134-42(2010 Apr)
Liu S,Yamashita H,Weidow B,Weaver AM,Quaranta V


book

Transcriptome-based systematic identification of extracellular matrix proteins.
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)
Manabe R,Tsutsui K,Yamada T,Kimura M,Nakano I,Shimono C,Sanzen N,Furutani Y,Fukuda T,Oguri Y,Shimamoto K,Kiyozumi D,Sato Y,Sado Y,Senoo H,Yamashina S,Fukuda S,Kawai J,Sugiura N,Kimata K,Hayashizaki Y,Sekiguchi K


book

Wdnm1-like, a new adipokine with a role in MMP-2 activation.
American journal of physiology. Endocrinology and metabolism295(1)E205-15(2008 Jul)
Wu Y,Smas CM