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Spry2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Spry2
Entrez Gene ID 306141
Full Name sprouty RTK signaling antagonist 2
General protein information
Preferred Names

sprouty RTK signaling antagonist 2

Names

protein sprouty homolog 2
sprouty 2
sprouty homolog 2
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

15

15q22

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001012046.1 NP_001012046.1 protein sprouty homolog 2
XM_006252438.3 XP_006252500.1 protein sprouty homolog 2 isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SPRY2 XP_003952460.1
Bos taurus (cattle) SPRY2 NP_001069615.1
SPRY2 XP_001090386.2
Canis lupus familiaris (dog) SPRY2 XP_005634066.1
Rattus norvegicus (Norway rat) Spry2 NP_001012046.1
Mus musculus (house mouse) Spry2 NP_036027.1
Danio rerio (zebrafish) spry2 NP_001003842.2
Xenopus tropicalis (tropical clawed frog) spry2 NP_001006932.2
Homo sapiens (human) SPRY2 NP_005833.1
Gallus gallus (chicken) SPRY2 NP_990131.1

Gene Ontology


Bibliography

Related articles in PubMed

Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.
Yim DG, Ghosh S, Guy GR, Virshup DM
Oncogene34(4)474-84(2015 Jan)

Sprouty2 in the dorsal hippocampus regulates neurogenesis and stress responsiveness in rats.
Dow AL, Lin TV, Chartoff EH, Potter D, McPhie DL, Van't Veer AV, Knoll AT, Lee KN, Neve RL, Patel TB, Ongur D, Cohen BM, Carlezon WA Jr
PloS one10(3)e0120693(2015)

MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
Wang JM, Tao J, Chen DD, Cai JJ, Irani K, Wang Q, Yuan H, Chen AF
Arteriosclerosis, thrombosis, and vascular biology34(1)99-109(2014 Jan)

Spatiotemporal alterations in Sprouty-2 expression and tyrosine phosphorylation in nitrofen-induced pulmonary hypoplasia.
Friedmacher F, Gosemann JH, Fujiwara N, Alvarez LA, Corcionivoschi N, Puri P
Journal of pediatric surgery48(11)2219-25(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CK1 activity and binding are necessary for SPRY2 inhibition of FGF-stimulated neurite outgrowth in PC12 cells
Title: Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.

SPRY2 negatively regulates fibroblast growth factor-2.
Title: Sprouty2 in the dorsal hippocampus regulates neurogenesis and stress responsiveness in rats.

Decreased expression of Sproutys and SPREDs during the terminal pseudoglandular stage may disrupt lung branching morphogenesis by interfering with epithelial-mesenchymal interactions contributing to PH.
Title: Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen-induced pulmonary hypoplasia.

Spry2 mRNA, protein, and tyrosination on D18 and D21 were significantly decreased in hypoplastic lungs with and without congenital diaphragmatic hernia.
Title: Spatiotemporal alterations in Sprouty-2 expression and tyrosine phosphorylation in nitrofen-induced pulmonary hypoplasia.

A comparison the distribution of Sprouty-1 and Sprouty-2 proteins by immunohistochemistry in developing and adult rat lung.
Title: Immunolocalization of sprouty-1 and sprouty-2 in developing rat lung.

The following Spry2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spry2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa02050 NM_001012046.1
Latest version!		 Rattus norvegicus sprouty RTK signaling antagonist 2 (Spry2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
ORa02050 XM_006252438.3
Latest version!		 Rattus norvegicus sprouty RTK signaling antagonist 2 (Spry2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa02050
Clone ID Related Accession (Same CDS sequence) NM_001012046.1 , XM_006252438.3
Accession Version NM_001012046.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-18
Organism Rattus norvegicus(Norway rat)
Product protein sprouty homolog 2
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089116.1. On Feb 11, 2005 this sequence version replaced XM_224515.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089116.1, CO399410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGGCCA GAGCTCAGAG CGGCAGCGGG TCGCAGCCTT TGCTGCAGTC ACCCCATGAC 
AGTGGCAGGC AGCGTGGGGA GCCGGACCCC AGAGATGCCC TTACCCAGCA GGTACATGTC 
TTGTCTCTGG ATCAGATCAG AGCCATCCGA AACACCAATG AGTACACAGA GGGGCCTACT 
GTGGTCCCAA GACCTGGGCT CAAGCCTGCT CCTCGCCCCT CCACCCAGCA CAAACATGAA 
AGACTCCACG GTCTGCCCGA GCACCGCCAG CCTCCCAGGC TCCAGCCCAC GCAGGTCCAT 
TCTTCCCGGG CCGCTCTGTC CAGGTCCGTC AGCACCGTCA GCTCAGGGTC TCGGAGCAGT 
ACAAGGACAA GTACCAGCAG CAGCTCTTCG GAACAGAGAC TGTTAGGATC ATCCTTCTCC 
CCTGGGCCTG CTGTTGATGG GATAATCCGA GTGCAGCCTA AATCTGAGCT CAAATCCGGT 
GAGCTTAAGC CACTGAGCAG AGACGATTTG GGTCTGCATG CATACAGGTG TGAGGACTGT 
GGCAAGTGCA AGTGTAAGGA GTGCACCTAC CCGAGGCCCC TGCCATCGGA CTGGATCTGT 
GACAAGCAGT GCCTTTGCTC AGCCCAGAAC GTCATTGACT ATGGGACTTG TGTGTGCTGT 
GTGAAAGGTC TCTTCTATCA CTGCTCCAAT GACGACGAGG ACAATTGTGC TGACAACCCG 
TGTTCTTGCA GCCAGTCTCA CTGTTGTACG CGATGGTCAG CGATGGGTGT CATGTCCCTC 
TTTCTGCCTT GTTTATGGTG TTACCTTCCA GCCAAGGGTT GCCTTAAATT GTGCCAGGGG 
TGTTATGACC GAGTGAACAG GCCTGGATGT CGTTGTAAAA ACTCAAATAC AGTTTGCTGC 
AAAGTTCCCA CTGTCCCCCC AAGGAACTTT GAAAAGCCGA CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001012046.1
CDS218..1162
Translation

Target ORF information:

RefSeq Version NM_001012046.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus sprouty RTK signaling antagonist 2 (Spry2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001012046.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGGCCA GAGCTCAGAG CGGCAGCGGG TCGCAGCCTT TGCTGCAGTC ACCCCATGAC 
AGTGGCAGGC AGCGTGGGGA GCCGGACCCC AGAGATGCCC TTACCCAGCA GGTACATGTC 
TTGTCTCTGG ATCAGATCAG AGCCATCCGA AACACCAATG AGTACACAGA GGGGCCTACT 
GTGGTCCCAA GACCTGGGCT CAAGCCTGCT CCTCGCCCCT CCACCCAGCA CAAACATGAA 
AGACTCCACG GTCTGCCCGA GCACCGCCAG CCTCCCAGGC TCCAGCCCAC GCAGGTCCAT 
TCTTCCCGGG CCGCTCTGTC CAGGTCCGTC AGCACCGTCA GCTCAGGGTC TCGGAGCAGT 
ACAAGGACAA GTACCAGCAG CAGCTCTTCG GAACAGAGAC TGTTAGGATC ATCCTTCTCC 
CCTGGGCCTG CTGTTGATGG GATAATCCGA GTGCAGCCTA AATCTGAGCT CAAATCCGGT 
GAGCTTAAGC CACTGAGCAG AGACGATTTG GGTCTGCATG CATACAGGTG TGAGGACTGT 
GGCAAGTGCA AGTGTAAGGA GTGCACCTAC CCGAGGCCCC TGCCATCGGA CTGGATCTGT 
GACAAGCAGT GCCTTTGCTC AGCCCAGAAC GTCATTGACT ATGGGACTTG TGTGTGCTGT 
GTGAAAGGTC TCTTCTATCA CTGCTCCAAT GACGACGAGG ACAATTGTGC TGACAACCCG 
TGTTCTTGCA GCCAGTCTCA CTGTTGTACG CGATGGTCAG CGATGGGTGT CATGTCCCTC 
TTTCTGCCTT GTTTATGGTG TTACCTTCCA GCCAAGGGTT GCCTTAAATT GTGCCAGGGG 
TGTTATGACC GAGTGAACAG GCCTGGATGT CGTTGTAAAA ACTCAAATAC AGTTTGCTGC 
AAAGTTCCCA CTGTCCCCCC AAGGAACTTT GAAAAGCCGA CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa02050
Clone ID Related Accession (Same CDS sequence) NM_001012046.1 , XM_006252438.3
Accession Version XM_006252438.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product protein sprouty homolog 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGGCCA GAGCTCAGAG CGGCAGCGGG TCGCAGCCTT TGCTGCAGTC ACCCCATGAC 
AGTGGCAGGC AGCGTGGGGA GCCGGACCCC AGAGATGCCC TTACCCAGCA GGTACATGTC 
TTGTCTCTGG ATCAGATCAG AGCCATCCGA AACACCAATG AGTACACAGA GGGGCCTACT 
GTGGTCCCAA GACCTGGGCT CAAGCCTGCT CCTCGCCCCT CCACCCAGCA CAAACATGAA 
AGACTCCACG GTCTGCCCGA GCACCGCCAG CCTCCCAGGC TCCAGCCCAC GCAGGTCCAT 
TCTTCCCGGG CCGCTCTGTC CAGGTCCGTC AGCACCGTCA GCTCAGGGTC TCGGAGCAGT 
ACAAGGACAA GTACCAGCAG CAGCTCTTCG GAACAGAGAC TGTTAGGATC ATCCTTCTCC 
CCTGGGCCTG CTGTTGATGG GATAATCCGA GTGCAGCCTA AATCTGAGCT CAAATCCGGT 
GAGCTTAAGC CACTGAGCAG AGACGATTTG GGTCTGCATG CATACAGGTG TGAGGACTGT 
GGCAAGTGCA AGTGTAAGGA GTGCACCTAC CCGAGGCCCC TGCCATCGGA CTGGATCTGT 
GACAAGCAGT GCCTTTGCTC AGCCCAGAAC GTCATTGACT ATGGGACTTG TGTGTGCTGT 
GTGAAAGGTC TCTTCTATCA CTGCTCCAAT GACGACGAGG ACAATTGTGC TGACAACCCG 
TGTTCTTGCA GCCAGTCTCA CTGTTGTACG CGATGGTCAG CGATGGGTGT CATGTCCCTC 
TTTCTGCCTT GTTTATGGTG TTACCTTCCA GCCAAGGGTT GCCTTAAATT GTGCCAGGGG 
TGTTATGACC GAGTGAACAG GCCTGGATGT CGTTGTAAAA ACTCAAATAC AGTTTGCTGC 
AAAGTTCCCA CTGTCCCCCC AAGGAACTTT GAAAAGCCGA CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006252500.1
CDS514..1458
Translation

Target ORF information:

RefSeq Version XM_006252438.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus sprouty RTK signaling antagonist 2 (Spry2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006252438.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGGCCA GAGCTCAGAG CGGCAGCGGG TCGCAGCCTT TGCTGCAGTC ACCCCATGAC 
AGTGGCAGGC AGCGTGGGGA GCCGGACCCC AGAGATGCCC TTACCCAGCA GGTACATGTC 
TTGTCTCTGG ATCAGATCAG AGCCATCCGA AACACCAATG AGTACACAGA GGGGCCTACT 
GTGGTCCCAA GACCTGGGCT CAAGCCTGCT CCTCGCCCCT CCACCCAGCA CAAACATGAA 
AGACTCCACG GTCTGCCCGA GCACCGCCAG CCTCCCAGGC TCCAGCCCAC GCAGGTCCAT 
TCTTCCCGGG CCGCTCTGTC CAGGTCCGTC AGCACCGTCA GCTCAGGGTC TCGGAGCAGT 
ACAAGGACAA GTACCAGCAG CAGCTCTTCG GAACAGAGAC TGTTAGGATC ATCCTTCTCC 
CCTGGGCCTG CTGTTGATGG GATAATCCGA GTGCAGCCTA AATCTGAGCT CAAATCCGGT 
GAGCTTAAGC CACTGAGCAG AGACGATTTG GGTCTGCATG CATACAGGTG TGAGGACTGT 
GGCAAGTGCA AGTGTAAGGA GTGCACCTAC CCGAGGCCCC TGCCATCGGA CTGGATCTGT 
GACAAGCAGT GCCTTTGCTC AGCCCAGAAC GTCATTGACT ATGGGACTTG TGTGTGCTGT 
GTGAAAGGTC TCTTCTATCA CTGCTCCAAT GACGACGAGG ACAATTGTGC TGACAACCCG 
TGTTCTTGCA GCCAGTCTCA CTGTTGTACG CGATGGTCAG CGATGGGTGT CATGTCCCTC 
TTTCTGCCTT GTTTATGGTG TTACCTTCCA GCCAAGGGTT GCCTTAAATT GTGCCAGGGG 
TGTTATGACC GAGTGAACAG GCCTGGATGT CGTTGTAAAA ACTCAAATAC AGTTTGCTGC 
AAAGTTCCCA CTGTCCCCCC AAGGAACTTT GAAAAGCCGA CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.
Oncogene34(4)474-84(2015 Jan)
Yim DG,Ghosh S,Guy GR,Virshup DM


book

Sprouty2 in the dorsal hippocampus regulates neurogenesis and stress responsiveness in rats.
PloS one10(3)e0120693(2015)
Dow AL,Lin TV,Chartoff EH,Potter D,McPhie DL,Van't Veer AV,Knoll AT,Lee KN,Neve RL,Patel TB,Ongur D,Cohen BM,Carlezon WA Jr


book

MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
Arteriosclerosis, thrombosis, and vascular biology34(1)99-109(2014 Jan)
Wang JM,Tao J,Chen DD,Cai JJ,i K,Wang Q,Yuan H,Chen AF


book

Spatiotemporal alterations in Sprouty-2 expression and tyrosine phosphorylation in nitrofen-induced pulmonary hypoplasia.
Journal of pediatric surgery48(11)2219-25(2013 Nov)
Friedmacher F,Gosemann JH,Fujiwara N,Alvarez LA,Corcionivoschi N,Puri P