Ank1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Ank1
Entrez Gene ID	306570
Full Name	ankyrin 1
General protein information	Preferred Names ankyrin 1 Names ankyrin-1 ankyrin 1, erythrocytic ankyrin 1, erythroid
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600148.1	XP_017455637.1	ankyrin-1 isoform X4
XM_008771358.2	XP_008769580.1	ankyrin-1 isoform X5
XM_008771356.2	XP_008769578.2	ankyrin-1 isoform X1
XM_008771357.2	XP_008769579.2	ankyrin-1 isoform X2
XM_008771359.2	XP_008769581.1	ankyrin-1 isoform X3
NM_001107322.2	NP_001100792.1	ankyrin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANK1	XP_005629869.1
Bos taurus (cattle)	ANK1	XP_002698771.3
Mus musculus (house mouse)	Ank1	NP_001104253.1
Homo sapiens (human)	ANK1	NP_001135918.1
Rattus norvegicus (Norway rat)	Ank1	NP_001100792.1
Pan troglodytes (chimpanzee)	ANK1	XP_003311743.1
	ANK1	XP_001099591.2
Gallus gallus (chicken)	ANK1	XP_424401.4
Danio rerio (zebrafish)	si:ch211-263m18.3	XP_005161295.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.
Desmond PF, Muriel J, Markwardt ML, Rizzo MA, Bloch RJ
The Journal of biological chemistry290(46)27854-67(2015 Nov)

A deep intronic mutation in the ankyrin-1 gene causes diminished protein expression resulting in hemolytic anemia in mice.
Huang H, Zhao P, Arimatsu K, Tabeta K, Yamazaki K, Krieg L, Fu E, Zhang T, Du X
G3 (Bethesda, Md.)3(10)1687-95(2013 Oct)

Suppression of hepcidin expression and iron overload mediate Salmonella susceptibility in ankyrin 1 ENU-induced mutant.
Yuki KE, Eva MM, Richer E, Chung D, Paquet M, Cellier M, Canonne-Hergaux F, Vaulont S, Vidal SM, Malo D
PloS one8(2)e55331(2013)

Hydrophobic residues in small ankyrin 1 participate in binding to obscurin.
Willis CD, Oashi T, Busby B, Mackerell AD Jr, Bloch RJ
Molecular membrane biology29(2)36-51(2012 Mar)

Integrity of the network sarcoplasmic reticulum in skeletal muscle requires small ankyrin 1.
Ackermann MA, Ziman AP, Strong J, Zhang Y, Hartford AK, Ward CW, Randall WR, Kontrogianni-Konstantopoulos A, Bloch RJ
Journal of cell science124(Pt 21)3619-30(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results suggest that sAnk1 interacts with SERCA1 through its TM and cytoplasmic domains to regulate SERCA1 activity and modulate sequestration of Ca(2+) in the sarcoplasmic reticulum lumen.
Title: Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.

Site-directed mutagenesis, blot overlays and surface plasmon resonance assays were used to study the contribution of the hydrophobic residues, V70, F71, I102 and I103 of small ankyrin-1 (sAnk1), to two different 30-mers of obscurin that bind sAnk1.
Title: Hydrophobic residues in small ankyrin 1 participate in binding to obscurin.

Ank1 stabilizes the network sarcoplasmic reticulum in skeletal muscl.
Title: Integrity of the network sarcoplasmic reticulum in skeletal muscle requires small ankyrin 1.

Obscurin and ank1.5 localization in developing rat diaphragm
Title: Localization of ank1.5 in the sarcoplasmic reticulum precedes that of SERCA and RyR: relationship with the organization of obscurin in developing sarcomeres.

The following Ank1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ank1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58833	XM_008771356.2 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa58834	XM_008771357.2 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa58835	XM_017600148.1 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa05288	NM_001107322.2 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa46426	XM_008771358.2 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa46427	XM_008771359.2 Latest version!	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ank1 ( XM_008771356.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769578.2 Rattus norvegicus ankyrin 1 (Ank1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58833
Clone ID Related Accession (Same CDS sequence)	XM_008771356.2
Accession Version	XM_008771356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTCCCC GATAAGGCCT TTTCTAGTTG CAGGGTAGTT 
GTGGCTAGTC TCCTGGGGCG AGCCTGTTCT TGCTGCTCTG ACAGCTCTCC CGCCCTCCGG 
CCCGCCGGCG GGGTCCTGGT CCTGAGCAAC AAGATGGGCT TCTGTAAAGC CGATGCTGCT 
ACCAGCTTTT TGCGGGCGGC ACGCTCAGGA AACCTGGACA AGGCTTTGGA TCACCTACGC 
AATGGAGTGG ACATTAACAC CTGTAACCAG AACGGGTTGA ATGGCTTGCA TCTGGCCTCC 
AAAGAAGGCC ATGTGAAGAT GGTGGTTGAA CTTCTGCACA AAGAGATCAT TCTAGAAACA 
ACAACCAAGA AGGGGAACAC TGCTCTGCAT ATCGCTGCCC TTGCTGGTCA GGACGAGGTG 
GTCCGGGAGC TGGTCAACTA TGGAGCCAAT GTCAATGCCC AGTCCCAGAA AGGCTTCACT 
CCCCTGTACA TGGCTGCTCA AGAGAATCAC TTGGAGGTGG TGAAGTTTCT CCTGGAGAAT 
GGAGCCAATC AGAATGTAGC CACAGAGGAT GGCTTCACTC CGCTGGCTGT GGCTCTGCAG 
CAGGGCCATG AGAATGTGGT GGCTCACCTC ATCAACTATG GAACGAAAGG AAAAGTGCGC 
CTCCCTGCCC TGCACATCGC GGCCCGCAAC GATGACACAC GGACAGCCGC AGTCCTTCTG 
CAGAATGACC CCAACCCAGA TGTGCTTTCC AAGACGGGAT TCACACCCCT CCACATCGCA 
GCACACTACG AGAACCTCAA CGTGGCCCAG CTGCTCCTCA ACCGGGGAGC CAGCGTCAAC 
TTCACACCTC AGAATGGTAT TACTCCACTG CACATCGCCT CCCGCAGGGG AAATGTGATC 
ATGGTGAGAC TCCTGCTGGA CCGAGGGGCT CAGATAGAAA CGAGGACCAA GGATGAACTG 
ACACCACTCC ACTGTGCAGC TCGGAATGGA CACGTGAGAA TCTCAGAGAT CCTGCTGGAC 
CACGGGGCAC CCATCCAAGC CAAAACCAAG AATGGCTTGT CCCCCATCCA CATGGCGGCT 
CAGGGAGACC ACCTCGACTG TGTCCGACTT CTATTGCAAT ACAACGCCGA GATAGATGAC 
ATCACCTTAG ATCACCTGAC TCCTCTCCAC GTGGCAGCCC ACTGCGGCCA CCACAGGGTG 
GCTAAGGTTC TTTTGGATAA AGGGGCCAAG CCGAACTCCA GAGCCCTGAA TGGCTTTACC 
CCACTACACA TCGCCTGCAA GAAGAACCAC ATACGTGTCA TGGAGCTGCT GCTGAAGACA 
GGAGCCTCTA TTGACGCAGT CACTGAGTCT GGCCTGACAC CTCTCCATGT AGCCTCCTTC 
ATGGGACACC TTCCTATTGT GAAGAACTTA TTACAGAGGG GAGCGTCACC CAATGTCTCC 
AATGTGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGAACTGCT CACATATCTT TAACGGGTGA CGAACTTGTC 
GGCTCGAAGG CTGAAAGGCG GGACTCCCGG GATGTGGGTG AGGAGAAAGA GCTGTTGGAT 
TTTGTGCCGA AGCTAGACCA AGTGGTGGAA TCCCCAGCCA TCCCAAGGAT CCCTTGTGTC 
ACGCCTGAGA CTGTTGTGAT CCGATCCGAA GATCAGGAAC AAGCATCTAA AGAGTATGAT 
GAGGACTCAC TCATTCCCAG TAGCCCAGCC ACAGAGACCT CAGACAACAT CAGCCCCGTG 
GCTAGCCCCG TCCACACAGG GTTTCTGGTG AGCTTCATGG TCGATGCCCG GGGAGGATCC 
ATGAGAGGAA GTCGTCATAA TGGCCTAAGG GTGGTGATCC CTCCCCGGAC ATGCGCAGCA 
CCCACTCGCA TCACCTGCCG CCTGGTTAAG CCCCAGAAGC TAAGCACACC GCCCCCGCTG 
GCTGAGGAGG AGGGCCTAGC CAGCAGGATC ATTGCACTGG GTCCTACGGG GGCCCAGTTC 
CTTAGCCCTG TGATCGTGGA AATCCCCCAC TTTGCCTCCC ATGGCCGCGG GGACCGTGAG 
CTGGTGGTTC TGAGGAGCGA GAACGGCTCC GTGTGGAAGG AGCACAAGAG CCGCTATGGA 
GAAAGCTACC TGGACCAGAT CCTGAACGGC ATGGACGAAG AGCTGGGGAG CCTGGAGGAG 
CTGGAGAAGA AGCGCGTGTG TCGCATCATT ACCACCGACT TCCCCCTGTA CTTTGTCATC 
ATGTCCCGGC TCTGCCAGGA CTACGACACC ATCGGTCCTG AAGGGGGCTC CCTGAGAAGC 
AAGCTGGTGC CCCTGGTACA GGCGACGTTC CCCGAAAACG CGGTGACCAA GAAAGTGAAG 
CTGGCTCTGC AGGCCCAGCC TGTCCCAGAC GAGCTGGTGA CCAAGCTCCT GGGCAACCAG 
GCCACCTTCA GCCCCATTGT TACTGTTGAG CCAAGGCGTC GGAAGTTCCA CCGTCCCATT 
GGGCTGCGAA TCCCACTCCC TCCCTCATGG ACTGACAACC CCAGGGACAG CGGGGAGGGA 
GACACCACCA GCCTACGCTT ACTGTGCAGC GTCATCGGTG GAACTGACCA AGCCCAGTGG 
GAAGATATAA CGGGAACAAC CAAGCTTGTA TACGCCAATG AGTGCGCCAA CTTTACCACC 
AACGTCTCAG CCAGATTCTG GCTGTCGGAC TGTCCTCGGA CCGCTGAGGC CGTACACTTT 
GCCACTCTCT TGTACAAAGA GCTCACGGCA GTTCCCTACA TGGCCAAGTT TGTCATTTTC 
GCCAAGATGA ACGACCCCCG GGAAGGACGG CTACGCTGCT ACTGCATGAC AGATGACAAG 
GTGGACAAGA CCCTGGAACA GCATGAAAAC TTCGTGGAGG TGGCCCGGAG CAGGGACATA 
GAGGTTCTAG AAGGGATGCC TCTGTTTGCA GAACTCTCTG GGAACTTGGT GCCTGTCAAG 
AAAGCGGCCC AGCAGCGAAG CTTCCATTTC CAGTCATTTC GAGAGAATCG CCTGGCCATC 
CCCGTGAAGG TGAGGGACAG CAGCCGCGAG CCAGGAGGGT TCTTGTCGTT CCTGCGGAAG 
CCGATGAAAT ATGAAGACGC ACAGCACATC CTCTGCCACT TGAATGTCAC CATGCCCCCC 
TGTACCAAGG GCAGTGGAGC AGAAGACAGG AGAAGGACCC TGACGCCCTT GACTCTTCGC 
TATAGCATTC TCAGCGAGTC CCGGCTGGGT TTTACCAGTG ACACAGACCG TGTTGAAATG 
AAGATGGCTG TCATCAGGGA GCACCTCGGC CTCAGCTGGG CAGGTGAGTT ATGGAATTTG 
GACAAAAAGA AAAATAGCCT GATATCTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769578.2
CDS	16..4488
Translation	MQAPPCTLVPDKAFSSCRVVVASLLGRACSCCSDSSPALRPAGGVLVLSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISLTGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAGELWNLDKKKNSLISM

Target ORF information:

RefSeq Version	XM_008771356.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771356.2

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTCCCC GATAAGGCCT TTTCTAGTTG CAGGGTAGTT 
GTGGCTAGTC TCCTGGGGCG AGCCTGTTCT TGCTGCTCTG ACAGCTCTCC CGCCCTCCGG 
CCCGCCGGCG GGGTCCTGGT CCTGAGCAAC AAGATGGGCT TCTGTAAAGC CGATGCTGCT 
ACCAGCTTTT TGCGGGCGGC ACGCTCAGGA AACCTGGACA AGGCTTTGGA TCACCTACGC 
AATGGAGTGG ACATTAACAC CTGTAACCAG AACGGGTTGA ATGGCTTGCA TCTGGCCTCC 
AAAGAAGGCC ATGTGAAGAT GGTGGTTGAA CTTCTGCACA AAGAGATCAT TCTAGAAACA 
ACAACCAAGA AGGGGAACAC TGCTCTGCAT ATCGCTGCCC TTGCTGGTCA GGACGAGGTG 
GTCCGGGAGC TGGTCAACTA TGGAGCCAAT GTCAATGCCC AGTCCCAGAA AGGCTTCACT 
CCCCTGTACA TGGCTGCTCA AGAGAATCAC TTGGAGGTGG TGAAGTTTCT CCTGGAGAAT 
GGAGCCAATC AGAATGTAGC CACAGAGGAT GGCTTCACTC CGCTGGCTGT GGCTCTGCAG 
CAGGGCCATG AGAATGTGGT GGCTCACCTC ATCAACTATG GAACGAAAGG AAAAGTGCGC 
CTCCCTGCCC TGCACATCGC GGCCCGCAAC GATGACACAC GGACAGCCGC AGTCCTTCTG 
CAGAATGACC CCAACCCAGA TGTGCTTTCC AAGACGGGAT TCACACCCCT CCACATCGCA 
GCACACTACG AGAACCTCAA CGTGGCCCAG CTGCTCCTCA ACCGGGGAGC CAGCGTCAAC 
TTCACACCTC AGAATGGTAT TACTCCACTG CACATCGCCT CCCGCAGGGG AAATGTGATC 
ATGGTGAGAC TCCTGCTGGA CCGAGGGGCT CAGATAGAAA CGAGGACCAA GGATGAACTG 
ACACCACTCC ACTGTGCAGC TCGGAATGGA CACGTGAGAA TCTCAGAGAT CCTGCTGGAC 
CACGGGGCAC CCATCCAAGC CAAAACCAAG AATGGCTTGT CCCCCATCCA CATGGCGGCT 
CAGGGAGACC ACCTCGACTG TGTCCGACTT CTATTGCAAT ACAACGCCGA GATAGATGAC 
ATCACCTTAG ATCACCTGAC TCCTCTCCAC GTGGCAGCCC ACTGCGGCCA CCACAGGGTG 
GCTAAGGTTC TTTTGGATAA AGGGGCCAAG CCGAACTCCA GAGCCCTGAA TGGCTTTACC 
CCACTACACA TCGCCTGCAA GAAGAACCAC ATACGTGTCA TGGAGCTGCT GCTGAAGACA 
GGAGCCTCTA TTGACGCAGT CACTGAGTCT GGCCTGACAC CTCTCCATGT AGCCTCCTTC 
ATGGGACACC TTCCTATTGT GAAGAACTTA TTACAGAGGG GAGCGTCACC CAATGTCTCC 
AATGTGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGAACTGCT CACATATCTT TAACGGGTGA CGAACTTGTC 
GGCTCGAAGG CTGAAAGGCG GGACTCCCGG GATGTGGGTG AGGAGAAAGA GCTGTTGGAT 
TTTGTGCCGA AGCTAGACCA AGTGGTGGAA TCCCCAGCCA TCCCAAGGAT CCCTTGTGTC 
ACGCCTGAGA CTGTTGTGAT CCGATCCGAA GATCAGGAAC AAGCATCTAA AGAGTATGAT 
GAGGACTCAC TCATTCCCAG TAGCCCAGCC ACAGAGACCT CAGACAACAT CAGCCCCGTG 
GCTAGCCCCG TCCACACAGG GTTTCTGGTG AGCTTCATGG TCGATGCCCG GGGAGGATCC 
ATGAGAGGAA GTCGTCATAA TGGCCTAAGG GTGGTGATCC CTCCCCGGAC ATGCGCAGCA 
CCCACTCGCA TCACCTGCCG CCTGGTTAAG CCCCAGAAGC TAAGCACACC GCCCCCGCTG 
GCTGAGGAGG AGGGCCTAGC CAGCAGGATC ATTGCACTGG GTCCTACGGG GGCCCAGTTC 
CTTAGCCCTG TGATCGTGGA AATCCCCCAC TTTGCCTCCC ATGGCCGCGG GGACCGTGAG 
CTGGTGGTTC TGAGGAGCGA GAACGGCTCC GTGTGGAAGG AGCACAAGAG CCGCTATGGA 
GAAAGCTACC TGGACCAGAT CCTGAACGGC ATGGACGAAG AGCTGGGGAG CCTGGAGGAG 
CTGGAGAAGA AGCGCGTGTG TCGCATCATT ACCACCGACT TCCCCCTGTA CTTTGTCATC 
ATGTCCCGGC TCTGCCAGGA CTACGACACC ATCGGTCCTG AAGGGGGCTC CCTGAGAAGC 
AAGCTGGTGC CCCTGGTACA GGCGACGTTC CCCGAAAACG CGGTGACCAA GAAAGTGAAG 
CTGGCTCTGC AGGCCCAGCC TGTCCCAGAC GAGCTGGTGA CCAAGCTCCT GGGCAACCAG 
GCCACCTTCA GCCCCATTGT TACTGTTGAG CCAAGGCGTC GGAAGTTCCA CCGTCCCATT 
GGGCTGCGAA TCCCACTCCC TCCCTCATGG ACTGACAACC CCAGGGACAG CGGGGAGGGA 
GACACCACCA GCCTACGCTT ACTGTGCAGC GTCATCGGTG GAACTGACCA AGCCCAGTGG 
GAAGATATAA CGGGAACAAC CAAGCTTGTA TACGCCAATG AGTGCGCCAA CTTTACCACC 
AACGTCTCAG CCAGATTCTG GCTGTCGGAC TGTCCTCGGA CCGCTGAGGC CGTACACTTT 
GCCACTCTCT TGTACAAAGA GCTCACGGCA GTTCCCTACA TGGCCAAGTT TGTCATTTTC 
GCCAAGATGA ACGACCCCCG GGAAGGACGG CTACGCTGCT ACTGCATGAC AGATGACAAG 
GTGGACAAGA CCCTGGAACA GCATGAAAAC TTCGTGGAGG TGGCCCGGAG CAGGGACATA 
GAGGTTCTAG AAGGGATGCC TCTGTTTGCA GAACTCTCTG GGAACTTGGT GCCTGTCAAG 
AAAGCGGCCC AGCAGCGAAG CTTCCATTTC CAGTCATTTC GAGAGAATCG CCTGGCCATC 
CCCGTGAAGG TGAGGGACAG CAGCCGCGAG CCAGGAGGGT TCTTGTCGTT CCTGCGGAAG 
CCGATGAAAT ATGAAGACGC ACAGCACATC CTCTGCCACT TGAATGTCAC CATGCCCCCC 
TGTACCAAGG GCAGTGGAGC AGAAGACAGG AGAAGGACCC TGACGCCCTT GACTCTTCGC 
TATAGCATTC TCAGCGAGTC CCGGCTGGGT TTTACCAGTG ACACAGACCG TGTTGAAATG 
AAGATGGCTG TCATCAGGGA GCACCTCGGC CTCAGCTGGG CAGGTGAGTT ATGGAATTTG 
GACAAAAAGA AAAATAGCCT GATATCTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_008771357.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769579.2 Rattus norvegicus ankyrin 1 (Ank1), transcript variant X2, mRNA.

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CloneID	ORa58834
Clone ID Related Accession (Same CDS sequence)	XM_008771357.2
Accession Version	XM_008771357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCGC CGCCCTGCAC CCTGGTCCCC GATAAGGCCT TTTCTAGTTG CAGGGTAGTT 
GTGGCTAGTC TCCTGGGGCG AGCCTGTTCT TGCTGCTCTG ACAGCTCTCC CGCCCTCCGG 
CCCGCCGGCG GGGTCCTGGT CCTGAGCAAC AAGATGGGCT TCTGTAAAGC CGATGCTGCT 
ACCAGCTTTT TGCGGGCGGC ACGCTCAGGA AACCTGGACA AGGCTTTGGA TCACCTACGC 
AATGGAGTGG ACATTAACAC CTGTAACCAG AACGGGTTGA ATGGCTTGCA TCTGGCCTCC 
AAAGAAGGCC ATGTGAAGAT GGTGGTTGAA CTTCTGCACA AAGAGATCAT TCTAGAAACA 
ACAACCAAGA AGGGGAACAC TGCTCTGCAT ATCGCTGCCC TTGCTGGTCA GGACGAGGTG 
GTCCGGGAGC TGGTCAACTA TGGAGCCAAT GTCAATGCCC AGTCCCAGAA AGGCTTCACT 
CCCCTGTACA TGGCTGCTCA AGAGAATCAC TTGGAGGTGG TGAAGTTTCT CCTGGAGAAT 
GGAGCCAATC AGAATGTAGC CACAGAGGAT GGCTTCACTC CGCTGGCTGT GGCTCTGCAG 
CAGGGCCATG AGAATGTGGT GGCTCACCTC ATCAACTATG GAACGAAAGG AAAAGTGCGC 
CTCCCTGCCC TGCACATCGC GGCCCGCAAC GATGACACAC GGACAGCCGC AGTCCTTCTG 
CAGAATGACC CCAACCCAGA TGTGCTTTCC AAGACGGGAT TCACACCCCT CCACATCGCA 
GCACACTACG AGAACCTCAA CGTGGCCCAG CTGCTCCTCA ACCGGGGAGC CAGCGTCAAC 
TTCACACCTC AGAATGGTAT TACTCCACTG CACATCGCCT CCCGCAGGGG AAATGTGATC 
ATGGTGAGAC TCCTGCTGGA CCGAGGGGCT CAGATAGAAA CGAGGACCAA GGATGAACTG 
ACACCACTCC ACTGTGCAGC TCGGAATGGA CACGTGAGAA TCTCAGAGAT CCTGCTGGAC 
CACGGGGCAC CCATCCAAGC CAAAACCAAG AATGGCTTGT CCCCCATCCA CATGGCGGCT 
CAGGGAGACC ACCTCGACTG TGTCCGACTT CTATTGCAAT ACAACGCCGA GATAGATGAC 
ATCACCTTAG ATCACCTGAC TCCTCTCCAC GTGGCAGCCC ACTGCGGCCA CCACAGGGTG 
GCTAAGGTTC TTTTGGATAA AGGGGCCAAG CCGAACTCCA GAGCCCTGAA TGGCTTTACC 
CCACTACACA TCGCCTGCAA GAAGAACCAC ATACGTGTCA TGGAGCTGCT GCTGAAGACA 
GGAGCCTCTA TTGACGCAGT CACTGAGTCT GGCCTGACAC CTCTCCATGT AGCCTCCTTC 
ATGGGACACC TTCCTATTGT GAAGAACTTA TTACAGAGGG GAGCGTCACC CAATGTCTCC 
AATGTGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGTGACGAA CTTGTCGGCT CGAAGGCTGA AAGGCGGGAC 
TCCCGGGATG TGGGTGAGGA GAAAGAGCTG TTGGATTTTG TGCCGAAGCT AGACCAAGTG 
GTGGAATCCC CAGCCATCCC AAGGATCCCT TGTGTCACGC CTGAGACTGT TGTGATCCGA 
TCCGAAGATC AGGAACAAGC ATCTAAAGAG TATGATGAGG ACTCACTCAT TCCCAGTAGC 
CCAGCCACAG AGACCTCAGA CAACATCAGC CCCGTGGCTA GCCCCGTCCA CACAGGGTTT 
CTGGTGAGCT TCATGGTCGA TGCCCGGGGA GGATCCATGA GAGGAAGTCG TCATAATGGC 
CTAAGGGTGG TGATCCCTCC CCGGACATGC GCAGCACCCA CTCGCATCAC CTGCCGCCTG 
GTTAAGCCCC AGAAGCTAAG CACACCGCCC CCGCTGGCTG AGGAGGAGGG CCTAGCCAGC 
AGGATCATTG CACTGGGTCC TACGGGGGCC CAGTTCCTTA GCCCTGTGAT CGTGGAAATC 
CCCCACTTTG CCTCCCATGG CCGCGGGGAC CGTGAGCTGG TGGTTCTGAG GAGCGAGAAC 
GGCTCCGTGT GGAAGGAGCA CAAGAGCCGC TATGGAGAAA GCTACCTGGA CCAGATCCTG 
AACGGCATGG ACGAAGAGCT GGGGAGCCTG GAGGAGCTGG AGAAGAAGCG CGTGTGTCGC 
ATCATTACCA CCGACTTCCC CCTGTACTTT GTCATCATGT CCCGGCTCTG CCAGGACTAC 
GACACCATCG GTCCTGAAGG GGGCTCCCTG AGAAGCAAGC TGGTGCCCCT GGTACAGGCG 
ACGTTCCCCG AAAACGCGGT GACCAAGAAA GTGAAGCTGG CTCTGCAGGC CCAGCCTGTC 
CCAGACGAGC TGGTGACCAA GCTCCTGGGC AACCAGGCCA CCTTCAGCCC CATTGTTACT 
GTTGAGCCAA GGCGTCGGAA GTTCCACCGT CCCATTGGGC TGCGAATCCC ACTCCCTCCC 
TCATGGACTG ACAACCCCAG GGACAGCGGG GAGGGAGACA CCACCAGCCT ACGCTTACTG 
TGCAGCGTCA TCGGTGGAAC TGACCAAGCC CAGTGGGAAG ATATAACGGG AACAACCAAG 
CTTGTATACG CCAATGAGTG CGCCAACTTT ACCACCAACG TCTCAGCCAG ATTCTGGCTG 
TCGGACTGTC CTCGGACCGC TGAGGCCGTA CACTTTGCCA CTCTCTTGTA CAAAGAGCTC 
ACGGCAGTTC CCTACATGGC CAAGTTTGTC ATTTTCGCCA AGATGAACGA CCCCCGGGAA 
GGACGGCTAC GCTGCTACTG CATGACAGAT GACAAGGTGG ACAAGACCCT GGAACAGCAT 
GAAAACTTCG TGGAGGTGGC CCGGAGCAGG GACATAGAGG TTCTAGAAGG GATGCCTCTG 
TTTGCAGAAC TCTCTGGGAA CTTGGTGCCT GTCAAGAAAG CGGCCCAGCA GCGAAGCTTC 
CATTTCCAGT CATTTCGAGA GAATCGCCTG GCCATCCCCG TGAAGGTGAG GGACAGCAGC 
CGCGAGCCAG GAGGGTTCTT GTCGTTCCTG CGGAAGCCGA TGAAATATGA AGACGCACAG 
CACATCCTCT GCCACTTGAA TGTCACCATG CCCCCCTGTA CCAAGGGCAG TGGAGCAGAA 
GACAGGAGAA GGACCCTGAC GCCCTTGACT CTTCGCTATA GCATTCTCAG CGAGTCCCGG 
CTGGGTTTTA CCAGTGACAC AGACCGTGTT GAAATGAAGA TGGCTGTCAT CAGGGAGCAC 
CTCGGCCTCA GCTGGGCAGG TGAGTTATGG AATTTGGACA AAAAGAAAAA TAGCCTGATA 
TCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769579.2
CDS	16..4464
Translation	MQAPPCTLVPDKAFSSCRVVVASLLGRACSCCSDSSPALRPAGGVLVLSNKMGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAGELWNLDKKKNSLISM

Target ORF information:

RefSeq Version	XM_008771357.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771357.2

ORF Insert Sequence:

ATGCAGGCGC CGCCCTGCAC CCTGGTCCCC GATAAGGCCT TTTCTAGTTG CAGGGTAGTT 
GTGGCTAGTC TCCTGGGGCG AGCCTGTTCT TGCTGCTCTG ACAGCTCTCC CGCCCTCCGG 
CCCGCCGGCG GGGTCCTGGT CCTGAGCAAC AAGATGGGCT TCTGTAAAGC CGATGCTGCT 
ACCAGCTTTT TGCGGGCGGC ACGCTCAGGA AACCTGGACA AGGCTTTGGA TCACCTACGC 
AATGGAGTGG ACATTAACAC CTGTAACCAG AACGGGTTGA ATGGCTTGCA TCTGGCCTCC 
AAAGAAGGCC ATGTGAAGAT GGTGGTTGAA CTTCTGCACA AAGAGATCAT TCTAGAAACA 
ACAACCAAGA AGGGGAACAC TGCTCTGCAT ATCGCTGCCC TTGCTGGTCA GGACGAGGTG 
GTCCGGGAGC TGGTCAACTA TGGAGCCAAT GTCAATGCCC AGTCCCAGAA AGGCTTCACT 
CCCCTGTACA TGGCTGCTCA AGAGAATCAC TTGGAGGTGG TGAAGTTTCT CCTGGAGAAT 
GGAGCCAATC AGAATGTAGC CACAGAGGAT GGCTTCACTC CGCTGGCTGT GGCTCTGCAG 
CAGGGCCATG AGAATGTGGT GGCTCACCTC ATCAACTATG GAACGAAAGG AAAAGTGCGC 
CTCCCTGCCC TGCACATCGC GGCCCGCAAC GATGACACAC GGACAGCCGC AGTCCTTCTG 
CAGAATGACC CCAACCCAGA TGTGCTTTCC AAGACGGGAT TCACACCCCT CCACATCGCA 
GCACACTACG AGAACCTCAA CGTGGCCCAG CTGCTCCTCA ACCGGGGAGC CAGCGTCAAC 
TTCACACCTC AGAATGGTAT TACTCCACTG CACATCGCCT CCCGCAGGGG AAATGTGATC 
ATGGTGAGAC TCCTGCTGGA CCGAGGGGCT CAGATAGAAA CGAGGACCAA GGATGAACTG 
ACACCACTCC ACTGTGCAGC TCGGAATGGA CACGTGAGAA TCTCAGAGAT CCTGCTGGAC 
CACGGGGCAC CCATCCAAGC CAAAACCAAG AATGGCTTGT CCCCCATCCA CATGGCGGCT 
CAGGGAGACC ACCTCGACTG TGTCCGACTT CTATTGCAAT ACAACGCCGA GATAGATGAC 
ATCACCTTAG ATCACCTGAC TCCTCTCCAC GTGGCAGCCC ACTGCGGCCA CCACAGGGTG 
GCTAAGGTTC TTTTGGATAA AGGGGCCAAG CCGAACTCCA GAGCCCTGAA TGGCTTTACC 
CCACTACACA TCGCCTGCAA GAAGAACCAC ATACGTGTCA TGGAGCTGCT GCTGAAGACA 
GGAGCCTCTA TTGACGCAGT CACTGAGTCT GGCCTGACAC CTCTCCATGT AGCCTCCTTC 
ATGGGACACC TTCCTATTGT GAAGAACTTA TTACAGAGGG GAGCGTCACC CAATGTCTCC 
AATGTGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGTGACGAA CTTGTCGGCT CGAAGGCTGA AAGGCGGGAC 
TCCCGGGATG TGGGTGAGGA GAAAGAGCTG TTGGATTTTG TGCCGAAGCT AGACCAAGTG 
GTGGAATCCC CAGCCATCCC AAGGATCCCT TGTGTCACGC CTGAGACTGT TGTGATCCGA 
TCCGAAGATC AGGAACAAGC ATCTAAAGAG TATGATGAGG ACTCACTCAT TCCCAGTAGC 
CCAGCCACAG AGACCTCAGA CAACATCAGC CCCGTGGCTA GCCCCGTCCA CACAGGGTTT 
CTGGTGAGCT TCATGGTCGA TGCCCGGGGA GGATCCATGA GAGGAAGTCG TCATAATGGC 
CTAAGGGTGG TGATCCCTCC CCGGACATGC GCAGCACCCA CTCGCATCAC CTGCCGCCTG 
GTTAAGCCCC AGAAGCTAAG CACACCGCCC CCGCTGGCTG AGGAGGAGGG CCTAGCCAGC 
AGGATCATTG CACTGGGTCC TACGGGGGCC CAGTTCCTTA GCCCTGTGAT CGTGGAAATC 
CCCCACTTTG CCTCCCATGG CCGCGGGGAC CGTGAGCTGG TGGTTCTGAG GAGCGAGAAC 
GGCTCCGTGT GGAAGGAGCA CAAGAGCCGC TATGGAGAAA GCTACCTGGA CCAGATCCTG 
AACGGCATGG ACGAAGAGCT GGGGAGCCTG GAGGAGCTGG AGAAGAAGCG CGTGTGTCGC 
ATCATTACCA CCGACTTCCC CCTGTACTTT GTCATCATGT CCCGGCTCTG CCAGGACTAC 
GACACCATCG GTCCTGAAGG GGGCTCCCTG AGAAGCAAGC TGGTGCCCCT GGTACAGGCG 
ACGTTCCCCG AAAACGCGGT GACCAAGAAA GTGAAGCTGG CTCTGCAGGC CCAGCCTGTC 
CCAGACGAGC TGGTGACCAA GCTCCTGGGC AACCAGGCCA CCTTCAGCCC CATTGTTACT 
GTTGAGCCAA GGCGTCGGAA GTTCCACCGT CCCATTGGGC TGCGAATCCC ACTCCCTCCC 
TCATGGACTG ACAACCCCAG GGACAGCGGG GAGGGAGACA CCACCAGCCT ACGCTTACTG 
TGCAGCGTCA TCGGTGGAAC TGACCAAGCC CAGTGGGAAG ATATAACGGG AACAACCAAG 
CTTGTATACG CCAATGAGTG CGCCAACTTT ACCACCAACG TCTCAGCCAG ATTCTGGCTG 
TCGGACTGTC CTCGGACCGC TGAGGCCGTA CACTTTGCCA CTCTCTTGTA CAAAGAGCTC 
ACGGCAGTTC CCTACATGGC CAAGTTTGTC ATTTTCGCCA AGATGAACGA CCCCCGGGAA 
GGACGGCTAC GCTGCTACTG CATGACAGAT GACAAGGTGG ACAAGACCCT GGAACAGCAT 
GAAAACTTCG TGGAGGTGGC CCGGAGCAGG GACATAGAGG TTCTAGAAGG GATGCCTCTG 
TTTGCAGAAC TCTCTGGGAA CTTGGTGCCT GTCAAGAAAG CGGCCCAGCA GCGAAGCTTC 
CATTTCCAGT CATTTCGAGA GAATCGCCTG GCCATCCCCG TGAAGGTGAG GGACAGCAGC 
CGCGAGCCAG GAGGGTTCTT GTCGTTCCTG CGGAAGCCGA TGAAATATGA AGACGCACAG 
CACATCCTCT GCCACTTGAA TGTCACCATG CCCCCCTGTA CCAAGGGCAG TGGAGCAGAA 
GACAGGAGAA GGACCCTGAC GCCCTTGACT CTTCGCTATA GCATTCTCAG CGAGTCCCGG 
CTGGGTTTTA CCAGTGACAC AGACCGTGTT GAAATGAAGA TGGCTGTCAT CAGGGAGCAC 
CTCGGCCTCA GCTGGGCAGG TGAGTTATGG AATTTGGACA AAAAGAAAAA TAGCCTGATA 
TCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_017600148.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455637.1 Rattus norvegicus ankyrin 1 (Ank1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa58835
Clone ID Related Accession (Same CDS sequence)	XM_017600148.1
Accession Version	XM_017600148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTTTG CGGGCGGCAC GCTCAGGAAA CCTGGACAAG 
GCTTTGGATC ACCTACGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAT 
GGCTTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACAAC AACCAAGAAG GGGAACACTG CTCTGCATAT CGCTGCCCTT 
GCTGGTCAGG ACGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCCCAGAAAG GCTTCACTCC CCTGTACATG GCTGCTCAAG AGAATCACTT GGAGGTGGTG 
AAGTTTCTCC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAGGATGG CTTCACTCCG 
CTGGCTGTGG CTCTGCAGCA GGGCCATGAG AATGTGGTGG CTCACCTCAT CAACTATGGA 
ACGAAAGGAA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC ACACTACGAG AACCTCAACG TGGCCCAGCT GCTCCTCAAC 
CGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGTATTA CTCCACTGCA CATCGCCTCC 
CGCAGGGGAA ATGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAACTGAC ACCACTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCCATCCACA TGGCGGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AACGCCGAGA TAGATGACAT CACCTTAGAT CACCTGACTC CTCTCCACGT GGCAGCCCAC 
TGCGGCCACC ACAGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC GAACTCCAGA 
GCCCTGAATG GCTTTACCCC ACTACACATC GCCTGCAAGA AGAACCACAT ACGTGTCATG 
GAGCTGCTGC TGAAGACAGG AGCCTCTATT GACGCAGTCA CTGAGTCTGG CCTGACACCT 
CTCCATGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTATT ACAGAGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCAT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA TGCCAAGGCC 
AAGGACGACC AGACACCACT TCACTGTGCT GCTCGCATCG GCCACACGAG CATGGTGAAG 
CTCCTGCTGG AGAATGACGC CAGCCCCAAC CTGGCTACCA CTGCTGGCCA CACACCTCTG 
CACACCGCAG CCCGTGAGGG TCACGTGGAC ACAGCCCTGG CCCTGCTGGA AAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTGGCTGC CAAGTATGGG 
AAGGTCCGGC TGGCAGAGCT GCTGCTGGAA CATGATGCCC ATCCCAACGC GGCCGGAAAG 
AACGGCTTGA CTCCCCTGCA TGTGGCCGTC CATCACAACA ACTTGGACAT TGTCCAGCTT 
CTCCTTCCCC GAGGTGGCTC CCCGCACAGC CCTGCCTGGA ATGGCTACAC CCCTTTGCAC 
ATCGCAGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAATA CGGAGGGTCC 
GCGAATGCAG AGTCTGTACA AGGAGTGACC CCGCTTCACC TGGCTGCCCA AGAGGGCCAC 
ACGGAAATGG TCGCTCTTCT CCTGTCAAAG CAAGCCAATG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CTCTCCACCT GGTAGCACAA GAAGGCCATG TTCCAGTGGC GGATGTGCTG 
ATTAAACATG GCGTCACAGT GGATGCTACG ACCCGGATGG GTTACACCCC ACTCCACGTG 
GCCAGTCACT ACGGAAACAT CAAGCTGGTG AAGTTCCTGC TACAGCACCA GGCAGATGTC 
AACGCCAAGA CCAAGCTAGG GTACAGCCCC TTGCACCAGG CAGCTCAGCA AGGACACACG 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACAGTGG ATGAGATCCT GGATGTGTCT GAAGATGAAG GTGACGAACT TGTCGGCTCG 
AAGGCTGAAA GGCGGGACTC CCGGGATGTG GGTGAGGAGA AAGAGCTGTT GGATTTTGTG 
CCGAAGCTAG ACCAAGTGGT GGAATCCCCA GCCATCCCAA GGATCCCTTG TGTCACGCCT 
GAGACTGTTG TGATCCGATC CGAAGATCAG GAACAAGCAT CTAAAGAGTA TGATGAGGAC 
TCACTCATTC CCAGTAGCCC AGCCACAGAG ACCTCAGACA ACATCAGCCC CGTGGCTAGC 
CCCGTCCACA CAGGGTTTCT GGTGAGCTTC ATGGTCGATG CCCGGGGAGG ATCCATGAGA 
GGAAGTCGTC ATAATGGCCT AAGGGTGGTG ATCCCTCCCC GGACATGCGC AGCACCCACT 
CGCATCACCT GCCGCCTGGT TAAGCCCCAG AAGCTAAGCA CACCGCCCCC GCTGGCTGAG 
GAGGAGGGCC TAGCCAGCAG GATCATTGCA CTGGGTCCTA CGGGGGCCCA GTTCCTTAGC 
CCTGTGATCG TGGAAATCCC CCACTTTGCC TCCCATGGCC GCGGGGACCG TGAGCTGGTG 
GTTCTGAGGA GCGAGAACGG CTCCGTGTGG AAGGAGCACA AGAGCCGCTA TGGAGAAAGC 
TACCTGGACC AGATCCTGAA CGGCATGGAC GAAGAGCTGG GGAGCCTGGA GGAGCTGGAG 
AAGAAGCGCG TGTGTCGCAT CATTACCACC GACTTCCCCC TGTACTTTGT CATCATGTCC 
CGGCTCTGCC AGGACTACGA CACCATCGGT CCTGAAGGGG GCTCCCTGAG AAGCAAGCTG 
GTGCCCCTGG TACAGGCGAC GTTCCCCGAA AACGCGGTGA CCAAGAAAGT GAAGCTGGCT 
CTGCAGGCCC AGCCTGTCCC AGACGAGCTG GTGACCAAGC TCCTGGGCAA CCAGGCCACC 
TTCAGCCCCA TTGTTACTGT TGAGCCAAGG CGTCGGAAGT TCCACCGTCC CATTGGGCTG 
CGAATCCCAC TCCCTCCCTC ATGGACTGAC AACCCCAGGG ACAGCGGGGA GGGAGACACC 
ACCAGCCTAC GCTTACTGTG CAGCGTCATC GGTGGAACTG ACCAAGCCCA GTGGGAAGAT 
ATAACGGGAA CAACCAAGCT TGTATACGCC AATGAGTGCG CCAACTTTAC CACCAACGTC 
TCAGCCAGAT TCTGGCTGTC GGACTGTCCT CGGACCGCTG AGGCCGTACA CTTTGCCACT 
CTCTTGTACA AAGAGCTCAC GGCAGTTCCC TACATGGCCA AGTTTGTCAT TTTCGCCAAG 
ATGAACGACC CCCGGGAAGG ACGGCTACGC TGCTACTGCA TGACAGATGA CAAGGTGGAC 
AAGACCCTGG AACAGCATGA AAACTTCGTG GAGGTGGCCC GGAGCAGGGA CATAGAGGTT 
CTAGAAGGGA TGCCTCTGTT TGCAGAACTC TCTGGGAACT TGGTGCCTGT CAAGAAAGCG 
GCCCAGCAGC GAAGCTTCCA TTTCCAGTCA TTTCGAGAGA ATCGCCTGGC CATCCCCGTG 
AAGGTGAGGG ACAGCAGCCG CGAGCCAGGA GGGTTCTTGT CGTTCCTGCG GAAGCCGATG 
AAATATGAAG ACGCACAGCA CATCCTCTGC CACTTGAATG TCACCATGCC CCCCTGTACC 
AAGGGCAGTG GAGCAGAAGA CAGGAGAAGG ACCCTGACGC CCTTGACTCT TCGCTATAGC 
ATTCTCAGCG AGTCCCGGCT GGGTTTTACC AGTGACACAG ACCGTGTTGA AATGAAGATG 
GCTGTCATCA GGGAGCACCT CGGCCTCAGC TGGGCAGGTG AGTTATGGAA TTTGGACAAA 
AAGAAAAATA GCCTGATATC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455637.1
CDS	166..4572
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAGELWNLDKKKNSLISM

Target ORF information:

RefSeq Version	XM_017600148.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600148.1

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTTTG CGGGCGGCAC GCTCAGGAAA CCTGGACAAG 
GCTTTGGATC ACCTACGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAT 
GGCTTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACAAC AACCAAGAAG GGGAACACTG CTCTGCATAT CGCTGCCCTT 
GCTGGTCAGG ACGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCCCAGAAAG GCTTCACTCC CCTGTACATG GCTGCTCAAG AGAATCACTT GGAGGTGGTG 
AAGTTTCTCC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAGGATGG CTTCACTCCG 
CTGGCTGTGG CTCTGCAGCA GGGCCATGAG AATGTGGTGG CTCACCTCAT CAACTATGGA 
ACGAAAGGAA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC ACACTACGAG AACCTCAACG TGGCCCAGCT GCTCCTCAAC 
CGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGTATTA CTCCACTGCA CATCGCCTCC 
CGCAGGGGAA ATGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAACTGAC ACCACTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCCATCCACA TGGCGGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AACGCCGAGA TAGATGACAT CACCTTAGAT CACCTGACTC CTCTCCACGT GGCAGCCCAC 
TGCGGCCACC ACAGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC GAACTCCAGA 
GCCCTGAATG GCTTTACCCC ACTACACATC GCCTGCAAGA AGAACCACAT ACGTGTCATG 
GAGCTGCTGC TGAAGACAGG AGCCTCTATT GACGCAGTCA CTGAGTCTGG CCTGACACCT 
CTCCATGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTATT ACAGAGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCAT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA TGCCAAGGCC 
AAGGACGACC AGACACCACT TCACTGTGCT GCTCGCATCG GCCACACGAG CATGGTGAAG 
CTCCTGCTGG AGAATGACGC CAGCCCCAAC CTGGCTACCA CTGCTGGCCA CACACCTCTG 
CACACCGCAG CCCGTGAGGG TCACGTGGAC ACAGCCCTGG CCCTGCTGGA AAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTGGCTGC CAAGTATGGG 
AAGGTCCGGC TGGCAGAGCT GCTGCTGGAA CATGATGCCC ATCCCAACGC GGCCGGAAAG 
AACGGCTTGA CTCCCCTGCA TGTGGCCGTC CATCACAACA ACTTGGACAT TGTCCAGCTT 
CTCCTTCCCC GAGGTGGCTC CCCGCACAGC CCTGCCTGGA ATGGCTACAC CCCTTTGCAC 
ATCGCAGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAATA CGGAGGGTCC 
GCGAATGCAG AGTCTGTACA AGGAGTGACC CCGCTTCACC TGGCTGCCCA AGAGGGCCAC 
ACGGAAATGG TCGCTCTTCT CCTGTCAAAG CAAGCCAATG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CTCTCCACCT GGTAGCACAA GAAGGCCATG TTCCAGTGGC GGATGTGCTG 
ATTAAACATG GCGTCACAGT GGATGCTACG ACCCGGATGG GTTACACCCC ACTCCACGTG 
GCCAGTCACT ACGGAAACAT CAAGCTGGTG AAGTTCCTGC TACAGCACCA GGCAGATGTC 
AACGCCAAGA CCAAGCTAGG GTACAGCCCC TTGCACCAGG CAGCTCAGCA AGGACACACG 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACAGTGG ATGAGATCCT GGATGTGTCT GAAGATGAAG GTGACGAACT TGTCGGCTCG 
AAGGCTGAAA GGCGGGACTC CCGGGATGTG GGTGAGGAGA AAGAGCTGTT GGATTTTGTG 
CCGAAGCTAG ACCAAGTGGT GGAATCCCCA GCCATCCCAA GGATCCCTTG TGTCACGCCT 
GAGACTGTTG TGATCCGATC CGAAGATCAG GAACAAGCAT CTAAAGAGTA TGATGAGGAC 
TCACTCATTC CCAGTAGCCC AGCCACAGAG ACCTCAGACA ACATCAGCCC CGTGGCTAGC 
CCCGTCCACA CAGGGTTTCT GGTGAGCTTC ATGGTCGATG CCCGGGGAGG ATCCATGAGA 
GGAAGTCGTC ATAATGGCCT AAGGGTGGTG ATCCCTCCCC GGACATGCGC AGCACCCACT 
CGCATCACCT GCCGCCTGGT TAAGCCCCAG AAGCTAAGCA CACCGCCCCC GCTGGCTGAG 
GAGGAGGGCC TAGCCAGCAG GATCATTGCA CTGGGTCCTA CGGGGGCCCA GTTCCTTAGC 
CCTGTGATCG TGGAAATCCC CCACTTTGCC TCCCATGGCC GCGGGGACCG TGAGCTGGTG 
GTTCTGAGGA GCGAGAACGG CTCCGTGTGG AAGGAGCACA AGAGCCGCTA TGGAGAAAGC 
TACCTGGACC AGATCCTGAA CGGCATGGAC GAAGAGCTGG GGAGCCTGGA GGAGCTGGAG 
AAGAAGCGCG TGTGTCGCAT CATTACCACC GACTTCCCCC TGTACTTTGT CATCATGTCC 
CGGCTCTGCC AGGACTACGA CACCATCGGT CCTGAAGGGG GCTCCCTGAG AAGCAAGCTG 
GTGCCCCTGG TACAGGCGAC GTTCCCCGAA AACGCGGTGA CCAAGAAAGT GAAGCTGGCT 
CTGCAGGCCC AGCCTGTCCC AGACGAGCTG GTGACCAAGC TCCTGGGCAA CCAGGCCACC 
TTCAGCCCCA TTGTTACTGT TGAGCCAAGG CGTCGGAAGT TCCACCGTCC CATTGGGCTG 
CGAATCCCAC TCCCTCCCTC ATGGACTGAC AACCCCAGGG ACAGCGGGGA GGGAGACACC 
ACCAGCCTAC GCTTACTGTG CAGCGTCATC GGTGGAACTG ACCAAGCCCA GTGGGAAGAT 
ATAACGGGAA CAACCAAGCT TGTATACGCC AATGAGTGCG CCAACTTTAC CACCAACGTC 
TCAGCCAGAT TCTGGCTGTC GGACTGTCCT CGGACCGCTG AGGCCGTACA CTTTGCCACT 
CTCTTGTACA AAGAGCTCAC GGCAGTTCCC TACATGGCCA AGTTTGTCAT TTTCGCCAAG 
ATGAACGACC CCCGGGAAGG ACGGCTACGC TGCTACTGCA TGACAGATGA CAAGGTGGAC 
AAGACCCTGG AACAGCATGA AAACTTCGTG GAGGTGGCCC GGAGCAGGGA CATAGAGGTT 
CTAGAAGGGA TGCCTCTGTT TGCAGAACTC TCTGGGAACT TGGTGCCTGT CAAGAAAGCG 
GCCCAGCAGC GAAGCTTCCA TTTCCAGTCA TTTCGAGAGA ATCGCCTGGC CATCCCCGTG 
AAGGTGAGGG ACAGCAGCCG CGAGCCAGGA GGGTTCTTGT CGTTCCTGCG GAAGCCGATG 
AAATATGAAG ACGCACAGCA CATCCTCTGC CACTTGAATG TCACCATGCC CCCCTGTACC 
AAGGGCAGTG GAGCAGAAGA CAGGAGAAGG ACCCTGACGC CCTTGACTCT TCGCTATAGC 
ATTCTCAGCG AGTCCCGGCT GGGTTTTACC AGTGACACAG ACCGTGTTGA AATGAAGATG 
GCTGTCATCA GGGAGCACCT CGGCCTCAGC TGGGCAGGTG AGTTATGGAA TTTGGACAAA 
AAGAAAAATA GCCTGATATC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( NM_001107322.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100792.1 Rattus norvegicus ankyrin 1 (Ank1), mRNA.

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CloneID	ORa05288
Clone ID Related Accession (Same CDS sequence)	NM_001107322.2
Accession Version	NM_001107322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473970.2. On Mar 3, 2011 this sequence version replaced NM_001107322.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906351, SAMN01906353 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAAC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
TTGCGGGCGG CACGCTCAGG AAACCTGGAC AAGGCTTTGG ATCACCTACG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AATGGCTTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC AACAACCAAG 
GTAAGGGATG GCTTCACTCC GCTGGCTGTG GCTCTGCAGC AGGGCCATGA GAATGTGGTG 
GCTCACCTCA TCAACTATGG AACGAAAGGA AAAGTGCGCC TCCCTGCCCT GCACATCGCG 
GCCCGCAACG ATGACACACG GACAGCCGCA GTCCTTCTGC AGAATGACCC CAACCCAGAT 
GTGCTTTCCA AGACGGGATT CACACCCCTC CACATCGCAG CACACTACGA GAACCTCAAC 
GTGGCCCAGC TGCTCCTCAA CCGGGGAGCC AGCGTCAACT TCACACCTCA GAATGGTATT 
ACTCCACTGC ACATCGCCTC CCGCAGGGGA AATGTGATCA TGGTGAGACT CCTGCTGGAC 
CGAGGGGCTC AGATAGAAAC GAGGACCAAG GATGAACTGA CACCACTCCA CTGTGCAGCT 
CGGAATGGAC ACGTGAGAAT CTCAGAGATC CTGCTGGACC ACGGGGCACC CATCCAAGCC 
AAAACCAAGA ATGGCTTGTC CCCCATCCAC ATGGCGGCTC AGGGAGACCA CCTCGACTGT 
GTCCGACTTC TATTGCAATA CAACGCCGAG ATAGATGACA TCACCTTAGA TCACCTGACT 
CCTCTCCACG TGGCAGCCCA CTGCGGCCAC CACAGGGTGG CTAAGGTTCT TTTGGATAAA 
GGGGCCAAGC CGAACTCCAG AGCCCTGAAT GGCTTTACCC CACTACACAT CGCCTGCAAG 
AAGAACCACA TACGTGTCAT GGAGCTGCTG CTGAAGACAG GAGCCTCTAT TGACGCAGTC 
ACTGAGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGAACTGCT CACATATCTT TAACGGGTGA CGAACTTGTC 
GGCTCGAAGG CTGAAAGGCG GGACTCCCGG GATGTGGGTG AGGAGAAAGA GCTGTTGGAT 
TTTGTGCCGA AGCTAGACCA AGTGGTGGAA TCCCCAGCCA TCCCAAGGAT CCCTTGTGTC 
ACGCCTGAGA CTGTTGTGAT CCGATCCGAA GATCAGGAAC AAGCATCTAA AGAGTATGAT 
GAGGACTCAC TCATTCCCAG TAGCCCAGCC ACAGAGACCT CAGACAACAT CAGCCCCGTG 
GCTAGCCCCG TCCACACAGG GTTTCTGGTG AGCTTCATGG TCGATGCCCG GGGAGGATCC 
ATGAGAGGAA GTCGTCATAA TGGCCTAAGG GTGGTGATCC CTCCCCGGAC ATGCGCAGCA 
CCCACTCGCA TCACCTGCCG CCTGGTTAAG CCCCAGAAGC TAAGCACACC GCCCCCGCTG 
GCTGAGGAGG AGGGCCTAGC CAGCAGGATC ATTGCACTGG GTCCTACGGG GGCCCAGTTC 
CTTAGCCCTG TGATCGTGGA AATCCCCCAC TTTGCCTCCC ATGGCCGCGG GGACCGTGAG 
CTGGTGGTTC TGAGGAGCGA GAACGGCTCC GTGTGGAAGG AGCACAAGAG CCGCTATGGA 
GAAAGCTACC TGGACCAGAT CCTGAACGGC ATGGACGAAG AGCTGGGGAG CCTGGAGGAG 
CTGGAGAAGA AGCGCGTGTG TCGCATCATT ACCACCGACT TCCCCCTGTA CTTTGTCATC 
ATGTCCCGGC TCTGCCAGGA CTACGACACC ATCGGTCCTG AAGGGGGCTC CCTGAGAAGC 
AAGCTGGTGC CCCTGGTACA GGCGACGTTC CCCGAAAACG CGGTGACCAA GAAAGTGAAG 
CTGGCTCTGC AGGCCCAGCC TGTCCCAGAC GAGCTGGTGA CCAAGCTCCT GGGCAACCAG 
GCCACCTTCA GCCCCATTGT TACTGTTGAG CCAAGGCGTC GGAAGTTCCA CCGTCCCATT 
GGGCTGCGAA TCCCACTCCC TCCCTCATGG ACTGACAACC CCAGGGACAG CGGGGAGGGA 
GACACCACCA GCCTACGCTT ACTGTGCAGC GTCATCGGTG GAACTGACCA AGCCCAGTGG 
GAAGATATAA CGGGAACAAC CAAGCTTGTA TACGCCAATG AGTGCGCCAA CTTTACCACC 
AACGTCTCAG CCAGATTCTG GCTGTCGGAC TGTCCTCGGA CCGCTGAGGC CGTACACTTT 
GCCACTCTCT TGTACAAAGA GCTCACGGCA GTTCCCTACA TGGCCAAGTT TGTCATTTTC 
GCCAAGATGA ACGACCCCCG GGAAGGACGG CTACGCTGCT ACTGCATGAC AGATGACAAG 
GTGGACAAGA CCCTGGAACA GCATGAAAAC TTCGTGGAGG TGGCCCGGAG CAGGGACATA 
GAGGTTCTAG AAGGGATGCC TCTGTTTGCA GAACTCTCTG GGAACTTGGT GCCTGTCAAG 
AAAGCGGCCC AGCAGCGAAG CTTCCATTTC CAGTCATTTC GAGAGAATCG CCTGGCCATC 
CCCGTGAAGG TGAGGGACAG CAGCCGCGAG CCAGGAGGGT TCTTGTCGTT CCTGCGGAAG 
CCGATGAAAT ATGAAGACGC ACAGCACATC CTCTGCCACT TGAATGTCAC CATGCCCCCC 
TGTACCAAGG GCAGTGGAGC AGAAGACAGG AGAAGGACCC TGACGCCCTT GACTCTTCGC 
TATAGCATTC TCAGCGAGTC CCGGCTGGGT TTTACCAGTG ACACAGACCG TGTTGAAATG 
AAGATGGCTG TCATCAGGGA GCACCTCGGC CTCAGCTGGG CAGTGGAGAA TCTGTACACG 
GCCCTGCGGA ACATCGACAG AAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA CCGGAGGCAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCACTAC 
GCACTTCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCCACGGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTTCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCCGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAGG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CAGTGGACTG GTATAAACAG 
GGTTCAGCAG CATCATATCC ACAAGAGGCC GCACAAAGTT CCTGGCAAGA GGAGGTCACG 
CAAGGCCCAC AGTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCCGGCACA 
CTTCAGGAAT ATGAGCAGGT GGTGGTGTCC ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGAGCGACA GCCCCAAGGC CGGGAAGGAC CCAAGTCTGT GGGCATCGGA GAGTGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACAGAGGAA 
CAATTCACAG ACGAACAGGG CAACATTGTC ACCAAGAAGA TCATTCGAAA AGTCGTCCGA 
CAGGTGGACT CCTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAAGGC AGGAAGGGGG CTCAAATAGT GAAGCGGGCC 
AGCTTGAAAA GGGGCAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100792.1
CDS	1..5124
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKVRDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTEKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISLTGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAVENLYTALRNIDRSEIVNMLEGSGRQSRNLKPDRRHGDREYSLSPSQVNGYSSLQDELLSPASLHYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSSGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTVDWYKQGSAASYPQEAAQSSWQEEVTQGPQSFQRRITTIQGPEPGTLQEYEQVVVSTREHVQRGPPESDSPKAGKDPSLWASESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Target ORF information:

RefSeq Version	NM_001107322.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107322.2

ORF Insert Sequence:

ATGGCAGAAC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
TTGCGGGCGG CACGCTCAGG AAACCTGGAC AAGGCTTTGG ATCACCTACG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AATGGCTTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC AACAACCAAG 
GTAAGGGATG GCTTCACTCC GCTGGCTGTG GCTCTGCAGC AGGGCCATGA GAATGTGGTG 
GCTCACCTCA TCAACTATGG AACGAAAGGA AAAGTGCGCC TCCCTGCCCT GCACATCGCG 
GCCCGCAACG ATGACACACG GACAGCCGCA GTCCTTCTGC AGAATGACCC CAACCCAGAT 
GTGCTTTCCA AGACGGGATT CACACCCCTC CACATCGCAG CACACTACGA GAACCTCAAC 
GTGGCCCAGC TGCTCCTCAA CCGGGGAGCC AGCGTCAACT TCACACCTCA GAATGGTATT 
ACTCCACTGC ACATCGCCTC CCGCAGGGGA AATGTGATCA TGGTGAGACT CCTGCTGGAC 
CGAGGGGCTC AGATAGAAAC GAGGACCAAG GATGAACTGA CACCACTCCA CTGTGCAGCT 
CGGAATGGAC ACGTGAGAAT CTCAGAGATC CTGCTGGACC ACGGGGCACC CATCCAAGCC 
AAAACCAAGA ATGGCTTGTC CCCCATCCAC ATGGCGGCTC AGGGAGACCA CCTCGACTGT 
GTCCGACTTC TATTGCAATA CAACGCCGAG ATAGATGACA TCACCTTAGA TCACCTGACT 
CCTCTCCACG TGGCAGCCCA CTGCGGCCAC CACAGGGTGG CTAAGGTTCT TTTGGATAAA 
GGGGCCAAGC CGAACTCCAG AGCCCTGAAT GGCTTTACCC CACTACACAT CGCCTGCAAG 
AAGAACCACA TACGTGTCAT GGAGCTGCTG CTGAAGACAG GAGCCTCTAT TGACGCAGTC 
ACTGAGAAAG TAGAAACCCC ATTGCACATG GCAGCCCGAG CAGGGCATAC AGAAGTGGCC 
AAATATTTGC TCCAGAACAA AGCCAAAGCC AATGCCAAGG CCAAGGACGA CCAGACACCA 
CTTCACTGTG CTGCTCGCAT CGGCCACACG AGCATGGTGA AGCTCCTGCT GGAGAATGAC 
GCCAGCCCCA ACCTGGCTAC CACTGCTGGC CACACACCTC TGCACACCGC AGCCCGTGAG 
GGTCACGTGG ACACAGCCCT GGCCCTGCTG GAAAAGGAGG CATCCCAAGC CTGCATGACC 
AAGAAAGGAT TTACCCCTCT CCACGTGGCT GCCAAGTATG GGAAGGTCCG GCTGGCAGAG 
CTGCTGCTGG AACATGATGC CCATCCCAAC GCGGCCGGAA AGAACGGCTT GACTCCCCTG 
CATGTGGCCG TCCATCACAA CAACTTGGAC ATTGTCCAGC TTCTCCTTCC CCGAGGTGGC 
TCCCCGCACA GCCCTGCCTG GAATGGCTAC ACCCCTTTGC ACATCGCAGC CAAGCAGAAC 
CAGATAGAGG TGGCCCGCAG TCTACTGCAA TACGGAGGGT CCGCGAATGC AGAGTCTGTA 
CAAGGAGTGA CCCCGCTTCA CCTGGCTGCC CAAGAGGGCC ACACGGAAAT GGTCGCTCTT 
CTCCTGTCAA AGCAAGCCAA TGGCAACCTG GGGAACAAGA GTGGACTCAC TCCTCTCCAC 
CTGGTAGCAC AAGAAGGCCA TGTTCCAGTG GCGGATGTGC TGATTAAACA TGGCGTCACA 
GTGGATGCTA CGACCCGGAT GGGTTACACC CCACTCCACG TGGCCAGTCA CTACGGAAAC 
ATCAAGCTGG TGAAGTTCCT GCTACAGCAC CAGGCAGATG TCAACGCCAA GACCAAGCTA 
GGGTACAGCC CCTTGCACCA GGCAGCTCAG CAAGGACACA CGGACATTGT GACATTGCTC 
CTGAAGAATG GTGCTTCTCC AAATGAGGTC AGCTCGAACG GAACCACACC TCTGGCAATA 
GCCAAACGTT TGGGCTACAT CTCTGTAACA GACGTGCTCA AGGTGGTCAC AGATGAAACC 
AGTGTGGTGT TAGTCAGTGA CAAACACCGG ATGAGCTACC CTGAGACAGT GGATGAGATC 
CTGGATGTGT CTGAAGATGA AGGAACTGCT CACATATCTT TAACGGGTGA CGAACTTGTC 
GGCTCGAAGG CTGAAAGGCG GGACTCCCGG GATGTGGGTG AGGAGAAAGA GCTGTTGGAT 
TTTGTGCCGA AGCTAGACCA AGTGGTGGAA TCCCCAGCCA TCCCAAGGAT CCCTTGTGTC 
ACGCCTGAGA CTGTTGTGAT CCGATCCGAA GATCAGGAAC AAGCATCTAA AGAGTATGAT 
GAGGACTCAC TCATTCCCAG TAGCCCAGCC ACAGAGACCT CAGACAACAT CAGCCCCGTG 
GCTAGCCCCG TCCACACAGG GTTTCTGGTG AGCTTCATGG TCGATGCCCG GGGAGGATCC 
ATGAGAGGAA GTCGTCATAA TGGCCTAAGG GTGGTGATCC CTCCCCGGAC ATGCGCAGCA 
CCCACTCGCA TCACCTGCCG CCTGGTTAAG CCCCAGAAGC TAAGCACACC GCCCCCGCTG 
GCTGAGGAGG AGGGCCTAGC CAGCAGGATC ATTGCACTGG GTCCTACGGG GGCCCAGTTC 
CTTAGCCCTG TGATCGTGGA AATCCCCCAC TTTGCCTCCC ATGGCCGCGG GGACCGTGAG 
CTGGTGGTTC TGAGGAGCGA GAACGGCTCC GTGTGGAAGG AGCACAAGAG CCGCTATGGA 
GAAAGCTACC TGGACCAGAT CCTGAACGGC ATGGACGAAG AGCTGGGGAG CCTGGAGGAG 
CTGGAGAAGA AGCGCGTGTG TCGCATCATT ACCACCGACT TCCCCCTGTA CTTTGTCATC 
ATGTCCCGGC TCTGCCAGGA CTACGACACC ATCGGTCCTG AAGGGGGCTC CCTGAGAAGC 
AAGCTGGTGC CCCTGGTACA GGCGACGTTC CCCGAAAACG CGGTGACCAA GAAAGTGAAG 
CTGGCTCTGC AGGCCCAGCC TGTCCCAGAC GAGCTGGTGA CCAAGCTCCT GGGCAACCAG 
GCCACCTTCA GCCCCATTGT TACTGTTGAG CCAAGGCGTC GGAAGTTCCA CCGTCCCATT 
GGGCTGCGAA TCCCACTCCC TCCCTCATGG ACTGACAACC CCAGGGACAG CGGGGAGGGA 
GACACCACCA GCCTACGCTT ACTGTGCAGC GTCATCGGTG GAACTGACCA AGCCCAGTGG 
GAAGATATAA CGGGAACAAC CAAGCTTGTA TACGCCAATG AGTGCGCCAA CTTTACCACC 
AACGTCTCAG CCAGATTCTG GCTGTCGGAC TGTCCTCGGA CCGCTGAGGC CGTACACTTT 
GCCACTCTCT TGTACAAAGA GCTCACGGCA GTTCCCTACA TGGCCAAGTT TGTCATTTTC 
GCCAAGATGA ACGACCCCCG GGAAGGACGG CTACGCTGCT ACTGCATGAC AGATGACAAG 
GTGGACAAGA CCCTGGAACA GCATGAAAAC TTCGTGGAGG TGGCCCGGAG CAGGGACATA 
GAGGTTCTAG AAGGGATGCC TCTGTTTGCA GAACTCTCTG GGAACTTGGT GCCTGTCAAG 
AAAGCGGCCC AGCAGCGAAG CTTCCATTTC CAGTCATTTC GAGAGAATCG CCTGGCCATC 
CCCGTGAAGG TGAGGGACAG CAGCCGCGAG CCAGGAGGGT TCTTGTCGTT CCTGCGGAAG 
CCGATGAAAT ATGAAGACGC ACAGCACATC CTCTGCCACT TGAATGTCAC CATGCCCCCC 
TGTACCAAGG GCAGTGGAGC AGAAGACAGG AGAAGGACCC TGACGCCCTT GACTCTTCGC 
TATAGCATTC TCAGCGAGTC CCGGCTGGGT TTTACCAGTG ACACAGACCG TGTTGAAATG 
AAGATGGCTG TCATCAGGGA GCACCTCGGC CTCAGCTGGG CAGTGGAGAA TCTGTACACG 
GCCCTGCGGA ACATCGACAG AAGTGAGATT GTTAACATGT TGGAGGGCTC CGGCAGGCAG 
AGCAGAAACC TCAAACCAGA CCGGAGGCAT GGGGACCGGG AGTACTCATT GTCACCCTCC 
CAGGTGAATG GTTACTCCTC GCTGCAGGAC GAGCTGCTGT CCCCCGCCTC CCTGCACTAC 
GCACTTCCCT CTCCACTGTG TGCAGACCAG TACTGGAACG AAGTGGCCGT CATAGACGCC 
ATCCCCCTGG CGGCCACGGA GCATGACACC ATGCTGGAGA TGTCTGACAT GCAGGTGTGG 
TCTTCGGGCC TCACACCCTC CCTGGTCACT GCTGAGGACT CCTCTCTGGA GTGCAGCAAG 
GCCGAGGACT CTGACGCCAT ACCAGAGTGG AAGTTGGAGG GGGCACACTC AGAGGACACG 
CAGGGCCCGG AGCTGGGCTC TCAGGACCTG GTGGAGGACG ACACAGTGGA TTCAGATGCC 
ACAAATGGCC TGGCAGATTT GCTAGGCCAG CAGCGAGTTC ACGCCCGAAT CACAGACTCA 
CCCTCAGTGA GGCAGGTGCT GGACAGAAGC CAGGCCAGAA CAGTGGACTG GTATAAACAG 
GGTTCAGCAG CATCATATCC ACAAGAGGCC GCACAAAGTT CCTGGCAAGA GGAGGTCACG 
CAAGGCCCAC AGTCATTCCA GAGAAGGATC ACCACCATCC AAGGGCCGGA GCCCGGCACA 
CTTCAGGAAT ATGAGCAGGT GGTGGTGTCC ACCAGGGAGC ATGTGCAGAG GGGGCCACCT 
GAGAGCGACA GCCCCAAGGC CGGGAAGGAC CCAAGTCTGT GGGCATCGGA GAGTGCCTTC 
TCTCAAGAGG TGCAGGGGGA TGAGCTTCAG AATATTCCAG GAGAGCAGGT GACAGAGGAA 
CAATTCACAG ACGAACAGGG CAACATTGTC ACCAAGAAGA TCATTCGAAA AGTCGTCCGA 
CAGGTGGACT CCTCTGGTGC CATCGACACC CAGCAGCACG AGGAGGTGGA GCTAAGAGGG 
AGTGGACTCC AGCCGGACCT GATAGAAGGC AGGAAGGGGG CTCAAATAGT GAAGCGGGCC 
AGCTTGAAAA GGGGCAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_008771358.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769580.1 Rattus norvegicus ankyrin 1 (Ank1), transcript variant X5, mRNA.

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CloneID	ORa46426
Clone ID Related Accession (Same CDS sequence)	XM_008771358.2
Accession Version	XM_008771358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
TTGCGGGCGG CACGCTCAGG AAACCTGGAC AAGGCTTTGG ATCACCTACG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AATGGCTTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC AACAACCAAG 
AAGGGGAACA CTGCTCTGCA TATCGCTGCC CTTGCTGGTC AGGACGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCCCAGA AAGGCTTCAC TCCCCTGTAC 
ATGGCTGCTC AAGAGAATCA CTTGGAGGTG GTGAAGTTTC TCCTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAGGA TGGCTTCACT CCGCTGGCTG TGGCTCTGCA GCAGGGCCAT 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGAACGAAAG GAAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCACACTAC 
GAGAACCTCA ACGTGGCCCA GCTGCTCCTC AACCGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGTA TTACTCCACT GCACATCGCC TCCCGCAGGG GAAATGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAACT GACACCACTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCCATCC ACATGGCGGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAACGCCG AGATAGATGA CATCACCTTA 
GATCACCTGA CTCCTCTCCA CGTGGCAGCC CACTGCGGCC ACCACAGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCGAACTCC AGAGCCCTGA ATGGCTTTAC CCCACTACAC 
ATCGCCTGCA AGAAGAACCA CATACGTGTC ATGGAGCTGC TGCTGAAGAC AGGAGCCTCT 
ATTGACGCAG TCACTGAGTC TGGCCTGACA CCTCTCCATG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ATTACAGAGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CATTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAATGCCAAG GCCAAGGACG ACCAGACACC ACTTCACTGT 
GCTGCTCGCA TCGGCCACAC GAGCATGGTG AAGCTCCTGC TGGAGAATGA CGCCAGCCCC 
AACCTGGCTA CCACTGCTGG CCACACACCT CTGCACACCG CAGCCCGTGA GGGTCACGTG 
GACACAGCCC TGGCCCTGCT GGAAAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTGGC TGCCAAGTAT GGGAAGGTCC GGCTGGCAGA GCTGCTGCTG 
GAACATGATG CCCATCCCAA CGCGGCCGGA AAGAACGGCT TGACTCCCCT GCATGTGGCC 
GTCCATCACA ACAACTTGGA CATTGTCCAG CTTCTCCTTC CCCGAGGTGG CTCCCCGCAC 
AGCCCTGCCT GGAATGGCTA CACCCCTTTG CACATCGCAG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA ATACGGAGGG TCCGCGAATG CAGAGTCTGT ACAAGGAGTG 
ACCCCGCTTC ACCTGGCTGC CCAAGAGGGC CACACGGAAA TGGTCGCTCT TCTCCTGTCA 
AAGCAAGCCA ATGGCAACCT GGGGAACAAG AGTGGACTCA CTCCTCTCCA CCTGGTAGCA 
CAAGAAGGCC ATGTTCCAGT GGCGGATGTG CTGATTAAAC ATGGCGTCAC AGTGGATGCT 
ACGACCCGGA TGGGTTACAC CCCACTCCAC GTGGCCAGTC ACTACGGAAA CATCAAGCTG 
GTGAAGTTCC TGCTACAGCA CCAGGCAGAT GTCAACGCCA AGACCAAGCT AGGGTACAGC 
CCCTTGCACC AGGCAGCTCA GCAAGGACAC ACGGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACAG TGGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT TTAACGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA ATCCCCAGCC ATCCCAAGGA TCCCTTGTGT CACGCCTGAG 
ACTGTTGTGA TCCGATCCGA AGATCAGGAA CAAGCATCTA AAGAGTATGA TGAGGACTCA 
CTCATTCCCA GTAGCCCAGC CACAGAGACC TCAGACAACA TCAGCCCCGT GGCTAGCCCC 
GTCCACACAG GGTTTCTGGT GAGCTTCATG GTCGATGCCC GGGGAGGATC CATGAGAGGA 
AGTCGTCATA ATGGCCTAAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTGGTTAA GCCCCAGAAG CTAAGCACAC CGCCCCCGCT GGCTGAGGAG 
GAGGGCCTAG CCAGCAGGAT CATTGCACTG GGTCCTACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA CTTTGCCTCC CATGGCCGCG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGCG AGAACGGCTC CGTGTGGAAG GAGCACAAGA GCCGCTATGG AGAAAGCTAC 
CTGGACCAGA TCCTGAACGG CATGGACGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAGCGCGTGT GTCGCATCAT TACCACCGAC TTCCCCCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGCT CCCTGAGAAG CAAGCTGGTG 
CCCCTGGTAC AGGCGACGTT CCCCGAAAAC GCGGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCAGA CGAGCTGGTG ACCAAGCTCC TGGGCAACCA GGCCACCTTC 
AGCCCCATTG TTACTGTTGA GCCAAGGCGT CGGAAGTTCC ACCGTCCCAT TGGGCTGCGA 
ATCCCACTCC CTCCCTCATG GACTGACAAC CCCAGGGACA GCGGGGAGGG AGACACCACC 
AGCCTACGCT TACTGTGCAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAGCTTGT ATACGCCAAT GAGTGCGCCA ACTTTACCAC CAACGTCTCA 
GCCAGATTCT GGCTGTCGGA CTGTCCTCGG ACCGCTGAGG CCGTACACTT TGCCACTCTC 
TTGTACAAAG AGCTCACGGC AGTTCCCTAC ATGGCCAAGT TTGTCATTTT CGCCAAGATG 
AACGACCCCC GGGAAGGACG GCTACGCTGC TACTGCATGA CAGATGACAA GGTGGACAAG 
ACCCTGGAAC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTA 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TGCCTGTCAA GAAAGCGGCC 
CAGCAGCGAA GCTTCCATTT CCAGTCATTT CGAGAGAATC GCCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTGCGGAA GCCGATGAAA 
TATGAAGACG CACAGCACAT CCTCTGCCAC TTGAATGTCA CCATGCCCCC CTGTACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACGCCCT TGACTCTTCG CTATAGCATT 
CTCAGCGAGT CCCGGCTGGG TTTTACCAGT GACACAGACC GTGTTGAAAT GAAGATGGCT 
GTCATCAGGG AGCACCTCGG CCTCAGCTGG GCAGGTGAGT TATGGAATTT GGACAAAAAG 
AAAAATAGCC TGATATCTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769580.1
CDS	1315..5658
Translation	MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISLTGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAGELWNLDKKKNSLISM

Target ORF information:

RefSeq Version	XM_008771358.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771358.2

ORF Insert Sequence:

ATGGCAGAAC GGCCGCGCAG GTCGGGGTCA GACCCCGCGG CCGATGCTGC TACCAGCTTT 
TTGCGGGCGG CACGCTCAGG AAACCTGGAC AAGGCTTTGG ATCACCTACG CAATGGAGTG 
GACATTAACA CCTGTAACCA GAACGGGTTG AATGGCTTGC ATCTGGCCTC CAAAGAAGGC 
CATGTGAAGA TGGTGGTTGA ACTTCTGCAC AAAGAGATCA TTCTAGAAAC AACAACCAAG 
AAGGGGAACA CTGCTCTGCA TATCGCTGCC CTTGCTGGTC AGGACGAGGT GGTCCGGGAG 
CTGGTCAACT ATGGAGCCAA TGTCAATGCC CAGTCCCAGA AAGGCTTCAC TCCCCTGTAC 
ATGGCTGCTC AAGAGAATCA CTTGGAGGTG GTGAAGTTTC TCCTGGAGAA TGGAGCCAAT 
CAGAATGTAG CCACAGAGGA TGGCTTCACT CCGCTGGCTG TGGCTCTGCA GCAGGGCCAT 
GAGAATGTGG TGGCTCACCT CATCAACTAT GGAACGAAAG GAAAAGTGCG CCTCCCTGCC 
CTGCACATCG CGGCCCGCAA CGATGACACA CGGACAGCCG CAGTCCTTCT GCAGAATGAC 
CCCAACCCAG ATGTGCTTTC CAAGACGGGA TTCACACCCC TCCACATCGC AGCACACTAC 
GAGAACCTCA ACGTGGCCCA GCTGCTCCTC AACCGGGGAG CCAGCGTCAA CTTCACACCT 
CAGAATGGTA TTACTCCACT GCACATCGCC TCCCGCAGGG GAAATGTGAT CATGGTGAGA 
CTCCTGCTGG ACCGAGGGGC TCAGATAGAA ACGAGGACCA AGGATGAACT GACACCACTC 
CACTGTGCAG CTCGGAATGG ACACGTGAGA ATCTCAGAGA TCCTGCTGGA CCACGGGGCA 
CCCATCCAAG CCAAAACCAA GAATGGCTTG TCCCCCATCC ACATGGCGGC TCAGGGAGAC 
CACCTCGACT GTGTCCGACT TCTATTGCAA TACAACGCCG AGATAGATGA CATCACCTTA 
GATCACCTGA CTCCTCTCCA CGTGGCAGCC CACTGCGGCC ACCACAGGGT GGCTAAGGTT 
CTTTTGGATA AAGGGGCCAA GCCGAACTCC AGAGCCCTGA ATGGCTTTAC CCCACTACAC 
ATCGCCTGCA AGAAGAACCA CATACGTGTC ATGGAGCTGC TGCTGAAGAC AGGAGCCTCT 
ATTGACGCAG TCACTGAGTC TGGCCTGACA CCTCTCCATG TAGCCTCCTT CATGGGACAC 
CTTCCTATTG TGAAGAACTT ATTACAGAGG GGAGCGTCAC CCAATGTCTC CAATGTGAAA 
GTAGAAACCC CATTGCACAT GGCAGCCCGA GCAGGGCATA CAGAAGTGGC CAAATATTTG 
CTCCAGAACA AAGCCAAAGC CAATGCCAAG GCCAAGGACG ACCAGACACC ACTTCACTGT 
GCTGCTCGCA TCGGCCACAC GAGCATGGTG AAGCTCCTGC TGGAGAATGA CGCCAGCCCC 
AACCTGGCTA CCACTGCTGG CCACACACCT CTGCACACCG CAGCCCGTGA GGGTCACGTG 
GACACAGCCC TGGCCCTGCT GGAAAAGGAG GCATCCCAAG CCTGCATGAC CAAGAAAGGA 
TTTACCCCTC TCCACGTGGC TGCCAAGTAT GGGAAGGTCC GGCTGGCAGA GCTGCTGCTG 
GAACATGATG CCCATCCCAA CGCGGCCGGA AAGAACGGCT TGACTCCCCT GCATGTGGCC 
GTCCATCACA ACAACTTGGA CATTGTCCAG CTTCTCCTTC CCCGAGGTGG CTCCCCGCAC 
AGCCCTGCCT GGAATGGCTA CACCCCTTTG CACATCGCAG CCAAGCAGAA CCAGATAGAG 
GTGGCCCGCA GTCTACTGCA ATACGGAGGG TCCGCGAATG CAGAGTCTGT ACAAGGAGTG 
ACCCCGCTTC ACCTGGCTGC CCAAGAGGGC CACACGGAAA TGGTCGCTCT TCTCCTGTCA 
AAGCAAGCCA ATGGCAACCT GGGGAACAAG AGTGGACTCA CTCCTCTCCA CCTGGTAGCA 
CAAGAAGGCC ATGTTCCAGT GGCGGATGTG CTGATTAAAC ATGGCGTCAC AGTGGATGCT 
ACGACCCGGA TGGGTTACAC CCCACTCCAC GTGGCCAGTC ACTACGGAAA CATCAAGCTG 
GTGAAGTTCC TGCTACAGCA CCAGGCAGAT GTCAACGCCA AGACCAAGCT AGGGTACAGC 
CCCTTGCACC AGGCAGCTCA GCAAGGACAC ACGGACATTG TGACATTGCT CCTGAAGAAT 
GGTGCTTCTC CAAATGAGGT CAGCTCGAAC GGAACCACAC CTCTGGCAAT AGCCAAACGT 
TTGGGCTACA TCTCTGTAAC AGACGTGCTC AAGGTGGTCA CAGATGAAAC CAGTGTGGTG 
TTAGTCAGTG ACAAACACCG GATGAGCTAC CCTGAGACAG TGGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAACTGC TCACATATCT TTAACGGGTG ACGAACTTGT CGGCTCGAAG 
GCTGAAAGGC GGGACTCCCG GGATGTGGGT GAGGAGAAAG AGCTGTTGGA TTTTGTGCCG 
AAGCTAGACC AAGTGGTGGA ATCCCCAGCC ATCCCAAGGA TCCCTTGTGT CACGCCTGAG 
ACTGTTGTGA TCCGATCCGA AGATCAGGAA CAAGCATCTA AAGAGTATGA TGAGGACTCA 
CTCATTCCCA GTAGCCCAGC CACAGAGACC TCAGACAACA TCAGCCCCGT GGCTAGCCCC 
GTCCACACAG GGTTTCTGGT GAGCTTCATG GTCGATGCCC GGGGAGGATC CATGAGAGGA 
AGTCGTCATA ATGGCCTAAG GGTGGTGATC CCTCCCCGGA CATGCGCAGC ACCCACTCGC 
ATCACCTGCC GCCTGGTTAA GCCCCAGAAG CTAAGCACAC CGCCCCCGCT GGCTGAGGAG 
GAGGGCCTAG CCAGCAGGAT CATTGCACTG GGTCCTACGG GGGCCCAGTT CCTTAGCCCT 
GTGATCGTGG AAATCCCCCA CTTTGCCTCC CATGGCCGCG GGGACCGTGA GCTGGTGGTT 
CTGAGGAGCG AGAACGGCTC CGTGTGGAAG GAGCACAAGA GCCGCTATGG AGAAAGCTAC 
CTGGACCAGA TCCTGAACGG CATGGACGAA GAGCTGGGGA GCCTGGAGGA GCTGGAGAAG 
AAGCGCGTGT GTCGCATCAT TACCACCGAC TTCCCCCTGT ACTTTGTCAT CATGTCCCGG 
CTCTGCCAGG ACTACGACAC CATCGGTCCT GAAGGGGGCT CCCTGAGAAG CAAGCTGGTG 
CCCCTGGTAC AGGCGACGTT CCCCGAAAAC GCGGTGACCA AGAAAGTGAA GCTGGCTCTG 
CAGGCCCAGC CTGTCCCAGA CGAGCTGGTG ACCAAGCTCC TGGGCAACCA GGCCACCTTC 
AGCCCCATTG TTACTGTTGA GCCAAGGCGT CGGAAGTTCC ACCGTCCCAT TGGGCTGCGA 
ATCCCACTCC CTCCCTCATG GACTGACAAC CCCAGGGACA GCGGGGAGGG AGACACCACC 
AGCCTACGCT TACTGTGCAG CGTCATCGGT GGAACTGACC AAGCCCAGTG GGAAGATATA 
ACGGGAACAA CCAAGCTTGT ATACGCCAAT GAGTGCGCCA ACTTTACCAC CAACGTCTCA 
GCCAGATTCT GGCTGTCGGA CTGTCCTCGG ACCGCTGAGG CCGTACACTT TGCCACTCTC 
TTGTACAAAG AGCTCACGGC AGTTCCCTAC ATGGCCAAGT TTGTCATTTT CGCCAAGATG 
AACGACCCCC GGGAAGGACG GCTACGCTGC TACTGCATGA CAGATGACAA GGTGGACAAG 
ACCCTGGAAC AGCATGAAAA CTTCGTGGAG GTGGCCCGGA GCAGGGACAT AGAGGTTCTA 
GAAGGGATGC CTCTGTTTGC AGAACTCTCT GGGAACTTGG TGCCTGTCAA GAAAGCGGCC 
CAGCAGCGAA GCTTCCATTT CCAGTCATTT CGAGAGAATC GCCTGGCCAT CCCCGTGAAG 
GTGAGGGACA GCAGCCGCGA GCCAGGAGGG TTCTTGTCGT TCCTGCGGAA GCCGATGAAA 
TATGAAGACG CACAGCACAT CCTCTGCCAC TTGAATGTCA CCATGCCCCC CTGTACCAAG 
GGCAGTGGAG CAGAAGACAG GAGAAGGACC CTGACGCCCT TGACTCTTCG CTATAGCATT 
CTCAGCGAGT CCCGGCTGGG TTTTACCAGT GACACAGACC GTGTTGAAAT GAAGATGGCT 
GTCATCAGGG AGCACCTCGG CCTCAGCTGG GCAGGTGAGT TATGGAATTT GGACAAAAAG 
AAAAATAGCC TGATATCTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank1 ( XM_008771359.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769581.1 Rattus norvegicus ankyrin 1 (Ank1), transcript variant X3, mRNA.

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CloneID	ORa46427
Clone ID Related Accession (Same CDS sequence)	XM_008771359.2
Accession Version	XM_008771359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTTTG CGGGCGGCAC GCTCAGGAAA CCTGGACAAG 
GCTTTGGATC ACCTACGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAT 
GGCTTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACAAC AACCAAGAAG GGGAACACTG CTCTGCATAT CGCTGCCCTT 
GCTGGTCAGG ACGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCCCAGAAAG GCTTCACTCC CCTGTACATG GCTGCTCAAG AGAATCACTT GGAGGTGGTG 
AAGTTTCTCC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAGGATGG CTTCACTCCG 
CTGGCTGTGG CTCTGCAGCA GGGCCATGAG AATGTGGTGG CTCACCTCAT CAACTATGGA 
ACGAAAGGAA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC ACACTACGAG AACCTCAACG TGGCCCAGCT GCTCCTCAAC 
CGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGTATTA CTCCACTGCA CATCGCCTCC 
CGCAGGGGAA ATGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAACTGAC ACCACTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCCATCCACA TGGCGGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AACGCCGAGA TAGATGACAT CACCTTAGAT CACCTGACTC CTCTCCACGT GGCAGCCCAC 
TGCGGCCACC ACAGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC GAACTCCAGA 
GCCCTGAATG GCTTTACCCC ACTACACATC GCCTGCAAGA AGAACCACAT ACGTGTCATG 
GAGCTGCTGC TGAAGACAGG AGCCTCTATT GACGCAGTCA CTGAGTCTGG CCTGACACCT 
CTCCATGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTATT ACAGAGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCAT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA TGCCAAGGCC 
AAGGACGACC AGACACCACT TCACTGTGCT GCTCGCATCG GCCACACGAG CATGGTGAAG 
CTCCTGCTGG AGAATGACGC CAGCCCCAAC CTGGCTACCA CTGCTGGCCA CACACCTCTG 
CACACCGCAG CCCGTGAGGG TCACGTGGAC ACAGCCCTGG CCCTGCTGGA AAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTGGCTGC CAAGTATGGG 
AAGGTCCGGC TGGCAGAGCT GCTGCTGGAA CATGATGCCC ATCCCAACGC GGCCGGAAAG 
AACGGCTTGA CTCCCCTGCA TGTGGCCGTC CATCACAACA ACTTGGACAT TGTCCAGCTT 
CTCCTTCCCC GAGGTGGCTC CCCGCACAGC CCTGCCTGGA ATGGCTACAC CCCTTTGCAC 
ATCGCAGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAATA CGGAGGGTCC 
GCGAATGCAG AGTCTGTACA AGGAGTGACC CCGCTTCACC TGGCTGCCCA AGAGGGCCAC 
ACGGAAATGG TCGCTCTTCT CCTGTCAAAG CAAGCCAATG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CTCTCCACCT GGTAGCACAA GAAGGCCATG TTCCAGTGGC GGATGTGCTG 
ATTAAACATG GCGTCACAGT GGATGCTACG ACCCGGATGG GTTACACCCC ACTCCACGTG 
GCCAGTCACT ACGGAAACAT CAAGCTGGTG AAGTTCCTGC TACAGCACCA GGCAGATGTC 
AACGCCAAGA CCAAGCTAGG GTACAGCCCC TTGCACCAGG CAGCTCAGCA AGGACACACG 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACAGTGG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAACTGCTCA CATATCTTTA 
ACGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAATC CCCAGCCATC 
CCAAGGATCC CTTGTGTCAC GCCTGAGACT GTTGTGATCC GATCCGAAGA TCAGGAACAA 
GCATCTAAAG AGTATGATGA GGACTCACTC ATTCCCAGTA GCCCAGCCAC AGAGACCTCA 
GACAACATCA GCCCCGTGGC TAGCCCCGTC CACACAGGGT TTCTGGTGAG CTTCATGGTC 
GATGCCCGGG GAGGATCCAT GAGAGGAAGT CGTCATAATG GCCTAAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TGGTTAAGCC CCAGAAGCTA 
AGCACACCGC CCCCGCTGGC TGAGGAGGAG GGCCTAGCCA GCAGGATCAT TGCACTGGGT 
CCTACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCACTT TGCCTCCCAT 
GGCCGCGGGG ACCGTGAGCT GGTGGTTCTG AGGAGCGAGA ACGGCTCCGT GTGGAAGGAG 
CACAAGAGCC GCTATGGAGA AAGCTACCTG GACCAGATCC TGAACGGCAT GGACGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAG CGCGTGTGTC GCATCATTAC CACCGACTTC 
CCCCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGCTCCC TGAGAAGCAA GCTGGTGCCC CTGGTACAGG CGACGTTCCC CGAAAACGCG 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCAGACGA GCTGGTGACC 
AAGCTCCTGG GCAACCAGGC CACCTTCAGC CCCATTGTTA CTGTTGAGCC AAGGCGTCGG 
AAGTTCCACC GTCCCATTGG GCTGCGAATC CCACTCCCTC CCTCATGGAC TGACAACCCC 
AGGGACAGCG GGGAGGGAGA CACCACCAGC CTACGCTTAC TGTGCAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AGCTTGTATA CGCCAATGAG 
TGCGCCAACT TTACCACCAA CGTCTCAGCC AGATTCTGGC TGTCGGACTG TCCTCGGACC 
GCTGAGGCCG TACACTTTGC CACTCTCTTG TACAAAGAGC TCACGGCAGT TCCCTACATG 
GCCAAGTTTG TCATTTTCGC CAAGATGAAC GACCCCCGGG AAGGACGGCT ACGCTGCTAC 
TGCATGACAG ATGACAAGGT GGACAAGACC CTGGAACAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTAGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTGC CTGTCAAGAA AGCGGCCCAG CAGCGAAGCT TCCATTTCCA GTCATTTCGA 
GAGAATCGCC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TGCGGAAGCC GATGAAATAT GAAGACGCAC AGCACATCCT CTGCCACTTG 
AATGTCACCA TGCCCCCCTG TACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACGCCCTTGA CTCTTCGCTA TAGCATTCTC AGCGAGTCCC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TTGAAATGAA GATGGCTGTC ATCAGGGAGC ACCTCGGCCT CAGCTGGGCA 
GGTGAGTTAT GGAATTTGGA CAAAAAGAAA AATAGCCTGA TATCTATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769581.1
CDS	167..4597
Translation	MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTSMVKLLLENDASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVQLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISLTGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKPMKYEDAQHILCHLNVTMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMKMAVIREHLGLSWAGELWNLDKKKNSLISM

Target ORF information:

RefSeq Version	XM_008771359.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 1 (Ank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771359.2

ORF Insert Sequence:

ATGGCTCAGG CGGCCAAACA GCTGAAGAAA ATCAAAGACA TCGAGGCGCA GGCCCTCCAG 
GAGCAGAAGG AGAAGGAGGA ATCCAACAGG AAGCGGCGGA ACCGCTCCCG TGACCGCAAG 
AAGAAGGCCG ATGCTGCTAC CAGCTTTTTG CGGGCGGCAC GCTCAGGAAA CCTGGACAAG 
GCTTTGGATC ACCTACGCAA TGGAGTGGAC ATTAACACCT GTAACCAGAA CGGGTTGAAT 
GGCTTGCATC TGGCCTCCAA AGAAGGCCAT GTGAAGATGG TGGTTGAACT TCTGCACAAA 
GAGATCATTC TAGAAACAAC AACCAAGAAG GGGAACACTG CTCTGCATAT CGCTGCCCTT 
GCTGGTCAGG ACGAGGTGGT CCGGGAGCTG GTCAACTATG GAGCCAATGT CAATGCCCAG 
TCCCAGAAAG GCTTCACTCC CCTGTACATG GCTGCTCAAG AGAATCACTT GGAGGTGGTG 
AAGTTTCTCC TGGAGAATGG AGCCAATCAG AATGTAGCCA CAGAGGATGG CTTCACTCCG 
CTGGCTGTGG CTCTGCAGCA GGGCCATGAG AATGTGGTGG CTCACCTCAT CAACTATGGA 
ACGAAAGGAA AAGTGCGCCT CCCTGCCCTG CACATCGCGG CCCGCAACGA TGACACACGG 
ACAGCCGCAG TCCTTCTGCA GAATGACCCC AACCCAGATG TGCTTTCCAA GACGGGATTC 
ACACCCCTCC ACATCGCAGC ACACTACGAG AACCTCAACG TGGCCCAGCT GCTCCTCAAC 
CGGGGAGCCA GCGTCAACTT CACACCTCAG AATGGTATTA CTCCACTGCA CATCGCCTCC 
CGCAGGGGAA ATGTGATCAT GGTGAGACTC CTGCTGGACC GAGGGGCTCA GATAGAAACG 
AGGACCAAGG ATGAACTGAC ACCACTCCAC TGTGCAGCTC GGAATGGACA CGTGAGAATC 
TCAGAGATCC TGCTGGACCA CGGGGCACCC ATCCAAGCCA AAACCAAGAA TGGCTTGTCC 
CCCATCCACA TGGCGGCTCA GGGAGACCAC CTCGACTGTG TCCGACTTCT ATTGCAATAC 
AACGCCGAGA TAGATGACAT CACCTTAGAT CACCTGACTC CTCTCCACGT GGCAGCCCAC 
TGCGGCCACC ACAGGGTGGC TAAGGTTCTT TTGGATAAAG GGGCCAAGCC GAACTCCAGA 
GCCCTGAATG GCTTTACCCC ACTACACATC GCCTGCAAGA AGAACCACAT ACGTGTCATG 
GAGCTGCTGC TGAAGACAGG AGCCTCTATT GACGCAGTCA CTGAGTCTGG CCTGACACCT 
CTCCATGTAG CCTCCTTCAT GGGACACCTT CCTATTGTGA AGAACTTATT ACAGAGGGGA 
GCGTCACCCA ATGTCTCCAA TGTGAAAGTA GAAACCCCAT TGCACATGGC AGCCCGAGCA 
GGGCATACAG AAGTGGCCAA ATATTTGCTC CAGAACAAAG CCAAAGCCAA TGCCAAGGCC 
AAGGACGACC AGACACCACT TCACTGTGCT GCTCGCATCG GCCACACGAG CATGGTGAAG 
CTCCTGCTGG AGAATGACGC CAGCCCCAAC CTGGCTACCA CTGCTGGCCA CACACCTCTG 
CACACCGCAG CCCGTGAGGG TCACGTGGAC ACAGCCCTGG CCCTGCTGGA AAAGGAGGCA 
TCCCAAGCCT GCATGACCAA GAAAGGATTT ACCCCTCTCC ACGTGGCTGC CAAGTATGGG 
AAGGTCCGGC TGGCAGAGCT GCTGCTGGAA CATGATGCCC ATCCCAACGC GGCCGGAAAG 
AACGGCTTGA CTCCCCTGCA TGTGGCCGTC CATCACAACA ACTTGGACAT TGTCCAGCTT 
CTCCTTCCCC GAGGTGGCTC CCCGCACAGC CCTGCCTGGA ATGGCTACAC CCCTTTGCAC 
ATCGCAGCCA AGCAGAACCA GATAGAGGTG GCCCGCAGTC TACTGCAATA CGGAGGGTCC 
GCGAATGCAG AGTCTGTACA AGGAGTGACC CCGCTTCACC TGGCTGCCCA AGAGGGCCAC 
ACGGAAATGG TCGCTCTTCT CCTGTCAAAG CAAGCCAATG GCAACCTGGG GAACAAGAGT 
GGACTCACTC CTCTCCACCT GGTAGCACAA GAAGGCCATG TTCCAGTGGC GGATGTGCTG 
ATTAAACATG GCGTCACAGT GGATGCTACG ACCCGGATGG GTTACACCCC ACTCCACGTG 
GCCAGTCACT ACGGAAACAT CAAGCTGGTG AAGTTCCTGC TACAGCACCA GGCAGATGTC 
AACGCCAAGA CCAAGCTAGG GTACAGCCCC TTGCACCAGG CAGCTCAGCA AGGACACACG 
GACATTGTGA CATTGCTCCT GAAGAATGGT GCTTCTCCAA ATGAGGTCAG CTCGAACGGA 
ACCACACCTC TGGCAATAGC CAAACGTTTG GGCTACATCT CTGTAACAGA CGTGCTCAAG 
GTGGTCACAG ATGAAACCAG TGTGGTGTTA GTCAGTGACA AACACCGGAT GAGCTACCCT 
GAGACAGTGG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAACTGCTCA CATATCTTTA 
ACGGGTGACG AACTTGTCGG CTCGAAGGCT GAAAGGCGGG ACTCCCGGGA TGTGGGTGAG 
GAGAAAGAGC TGTTGGATTT TGTGCCGAAG CTAGACCAAG TGGTGGAATC CCCAGCCATC 
CCAAGGATCC CTTGTGTCAC GCCTGAGACT GTTGTGATCC GATCCGAAGA TCAGGAACAA 
GCATCTAAAG AGTATGATGA GGACTCACTC ATTCCCAGTA GCCCAGCCAC AGAGACCTCA 
GACAACATCA GCCCCGTGGC TAGCCCCGTC CACACAGGGT TTCTGGTGAG CTTCATGGTC 
GATGCCCGGG GAGGATCCAT GAGAGGAAGT CGTCATAATG GCCTAAGGGT GGTGATCCCT 
CCCCGGACAT GCGCAGCACC CACTCGCATC ACCTGCCGCC TGGTTAAGCC CCAGAAGCTA 
AGCACACCGC CCCCGCTGGC TGAGGAGGAG GGCCTAGCCA GCAGGATCAT TGCACTGGGT 
CCTACGGGGG CCCAGTTCCT TAGCCCTGTG ATCGTGGAAA TCCCCCACTT TGCCTCCCAT 
GGCCGCGGGG ACCGTGAGCT GGTGGTTCTG AGGAGCGAGA ACGGCTCCGT GTGGAAGGAG 
CACAAGAGCC GCTATGGAGA AAGCTACCTG GACCAGATCC TGAACGGCAT GGACGAAGAG 
CTGGGGAGCC TGGAGGAGCT GGAGAAGAAG CGCGTGTGTC GCATCATTAC CACCGACTTC 
CCCCTGTACT TTGTCATCAT GTCCCGGCTC TGCCAGGACT ACGACACCAT CGGTCCTGAA 
GGGGGCTCCC TGAGAAGCAA GCTGGTGCCC CTGGTACAGG CGACGTTCCC CGAAAACGCG 
GTGACCAAGA AAGTGAAGCT GGCTCTGCAG GCCCAGCCTG TCCCAGACGA GCTGGTGACC 
AAGCTCCTGG GCAACCAGGC CACCTTCAGC CCCATTGTTA CTGTTGAGCC AAGGCGTCGG 
AAGTTCCACC GTCCCATTGG GCTGCGAATC CCACTCCCTC CCTCATGGAC TGACAACCCC 
AGGGACAGCG GGGAGGGAGA CACCACCAGC CTACGCTTAC TGTGCAGCGT CATCGGTGGA 
ACTGACCAAG CCCAGTGGGA AGATATAACG GGAACAACCA AGCTTGTATA CGCCAATGAG 
TGCGCCAACT TTACCACCAA CGTCTCAGCC AGATTCTGGC TGTCGGACTG TCCTCGGACC 
GCTGAGGCCG TACACTTTGC CACTCTCTTG TACAAAGAGC TCACGGCAGT TCCCTACATG 
GCCAAGTTTG TCATTTTCGC CAAGATGAAC GACCCCCGGG AAGGACGGCT ACGCTGCTAC 
TGCATGACAG ATGACAAGGT GGACAAGACC CTGGAACAGC ATGAAAACTT CGTGGAGGTG 
GCCCGGAGCA GGGACATAGA GGTTCTAGAA GGGATGCCTC TGTTTGCAGA ACTCTCTGGG 
AACTTGGTGC CTGTCAAGAA AGCGGCCCAG CAGCGAAGCT TCCATTTCCA GTCATTTCGA 
GAGAATCGCC TGGCCATCCC CGTGAAGGTG AGGGACAGCA GCCGCGAGCC AGGAGGGTTC 
TTGTCGTTCC TGCGGAAGCC GATGAAATAT GAAGACGCAC AGCACATCCT CTGCCACTTG 
AATGTCACCA TGCCCCCCTG TACCAAGGGC AGTGGAGCAG AAGACAGGAG AAGGACCCTG 
ACGCCCTTGA CTCTTCGCTA TAGCATTCTC AGCGAGTCCC GGCTGGGTTT TACCAGTGAC 
ACAGACCGTG TTGAAATGAA GATGGCTGTC ATCAGGGAGC ACCTCGGCCT CAGCTGGGCA 
GGTGAGTTAT GGAATTTGGA CAAAAAGAAA AATAGCCTGA TATCTATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.
The Journal of biological chemistry290(46)27854-67(2015 Nov)
Desmond PF,Muriel J,Markwardt ML,Rizzo MA,Bloch RJ

A deep intronic mutation in the ankyrin-1 gene causes diminished protein expression resulting in hemolytic anemia in mice.
G3 (Bethesda, Md.)3(10)1687-95(2013 Oct)
Huang H,Zhao P,Arimatsu K,Tabeta K,Yamazaki K,Krieg L,Fu E,Zhang T,Du X

Suppression of hepcidin expression and iron overload mediate Salmonella susceptibility in ankyrin 1 ENU-induced mutant.
PloS one8(2)e55331(2013)
Yuki KE,Eva MM,Richer E,Chung D,Paquet M,Cellier M,Canonne-Hergaux F,Vaulont S,Vidal SM,Malo D

Hydrophobic residues in small ankyrin 1 participate in binding to obscurin.
Molecular membrane biology29(2)36-51(2012 Mar)
Willis CD,Oashi T,Busby B,Mackerell AD Jr,Bloch RJ

Integrity of the network sarcoplasmic reticulum in skeletal muscle requires small ankyrin 1.
Journal of cell science124(Pt 21)3619-30(2011 Nov)
Ackermann MA,Ziman AP,Strong J,Zhang Y,Hartford AK,Ward CW,Randall WR,Kontrogianni-Konstantopoulos A,Bloch RJ

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Ank1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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