Tsku cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Tsku
Entrez Gene ID	308843
Full Name	tsukushi, small leucine rich proteoglycan
Synonyms	Eiih
General protein information	Preferred Names tsukushi, small leucine rich proteoglycan Names tsukushin early insulin-induced hepatic gene protein hepatic protein EIIH leucine-rich repeat-containing protein 54
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229759.3	XP_006229821.1	tsukushin isoform X1
XM_006229760.2	XP_006229822.1	tsukushin isoform X1
XM_006229761.3	XP_006229823.1	tsukushin isoform X1
NM_001009965.2	NP_001009965.1	tsukushin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tsku	NP_001009965.1
Gallus gallus (chicken)	TSKU	NP_001005346.1
Mus musculus (house mouse)	Tsku	NP_001162011.1
Canis lupus familiaris (dog)	TSKU	XP_005633522.1
Homo sapiens (human)	TSKU	NP_001245139.1
Xenopus tropicalis (tropical clawed frog)	tsku	NP_001011330.1
Pan troglodytes (chimpanzee)	TSKU	XP_508654.3
	TSKU	XP_002799755.1
Danio rerio (zebrafish)	tsku	NP_956027.1

Gene Ontology
Bibliography

Related articles in PubMed

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
Henke A, Grace OC, Ashley GR, Stewart GD, Riddick AC, Yeun H, O'Donnell M, Anderson RA, Thomson AA
PloS one7(8)e42516(2012)

Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4.
Ohta K, Ito A, Kuriyama S, Lupo G, Kosaka M, Ohnuma S, Nakagawa S, Tanaka H
Proceedings of the National Academy of Sciences of the United States of America108(36)14962-7(2011 Sep)

Tsukushi is required for anterior commissure formation in mouse brain.
Ito A, Shinmyo Y, Abe T, Oshima N, Tanaka H, Ohta K
Biochemical and biophysical research communications402(4)813-8(2010 Nov)

Identification of a novel rat hepatic gene induced early by insulin, independently of glucose.
Coffy S, Decaux JF, Girard J, de Keyzer Y, Asfari M
The Biochemical journal385(Pt 1)165-71(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human.
Title: Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.

Tissue distribution of EIIH in adult rats revealed a pattern of expression mainly in the liver, intestine and islets of Langerhans, closely following that of the Glut2 (glucose transporter 2), suggesting that it may play a role in carbohydrate metabolism
Title: Identification of a novel rat hepatic gene induced early by insulin, independently of glucose.

The following Tsku gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsku cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01309	NM_001009965.2 Latest version!	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa23136	XM_006229761.3 Latest version!	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23136	XM_006229759.3 Latest version!	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23136	XM_006229760.2 Latest version!	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tsku ( NM_001009965.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009965.1 Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), mRNA.

Price & Availability↑

CloneID	ORa01309
Clone ID Related Accession (Same CDS sequence)	NM_001009965.2
Accession Version	NM_001009965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	tsukushin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC100644.1. On Aug 7, 2005 this sequence version replaced NM_001009965.1. ##Evidence-Data-START## Transcript exon combination :: BC100644.1, DN936790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA TAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCGATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCGATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009965.1
CDS	143..1204
Translation	MLCTLFLLLLALGIVQTTRPCFPGCQCEEETFGLFDSFSLIRVDCSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLGGPGYTTLAGLDLSHNLLTSITPTAFSRLRYLESLDLSHNGLAALPAEVFTSSPLSDINLSHNRLREVSISAFTTHSQGRALHVDLSHNLIHRLLPYPARASLSAPTIQSLNLSWNRLRAVPDLRDLPLRYLSLDGNPLATINPGAFMGLAGLTHLSLASLQGILQLPPHGFRELPGLQVLDLSGNPKLKWAGAEVFSGLGLLQELDLSGSSLVPLPETLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSPKVVLHCGDTQESARGPDIL

Target ORF information:

RefSeq Version	NM_001009965.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009965.2

ORF Insert Sequence:

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA TAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCGATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCGATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsku ( XM_006229759.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229821.1 Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23136
Clone ID Related Accession (Same CDS sequence)	XM_006229759.3 , XM_006229760.2 , XM_006229761.3
Accession Version	XM_006229759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tsukushin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229821.1
CDS	158..1219
Translation	MLCTLFLLLLALGTVQTTRPCFPGCQCEEETFGLFDSFSLIRVDCSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLGGPGYTTLAGLDLSHNLLTSITPTAFSRLRYLESLDLSHNGLAALPAEVFTSSPLSDINLSHNRLREVSISAFTTHSQGRALHVDLSHNLIHRLLPYPARASLSAPTIQSLNLSWNRLRAVPNLRDLPLRYLSLDGNPLATINPGAFMGLAGLTHLSLASLQGILQLPPHGFRELPGLQVLDLSGNPKLKWAGAEVFSGLGLLQELDLSGSSLVPLPETLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSPKVVLHCGDTQESARGPDIL

Target ORF information:

RefSeq Version	XM_006229759.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229759.3

ORF Insert Sequence:

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsku ( XM_006229760.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229822.1 Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa23136
Clone ID Related Accession (Same CDS sequence)	XM_006229759.3 , XM_006229760.2 , XM_006229761.3
Accession Version	XM_006229760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tsukushin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006229760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229822.1
CDS	1036..2097
Translation	MLCTLFLLLLALGTVQTTRPCFPGCQCEEETFGLFDSFSLIRVDCSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLGGPGYTTLAGLDLSHNLLTSITPTAFSRLRYLESLDLSHNGLAALPAEVFTSSPLSDINLSHNRLREVSISAFTTHSQGRALHVDLSHNLIHRLLPYPARASLSAPTIQSLNLSWNRLRAVPNLRDLPLRYLSLDGNPLATINPGAFMGLAGLTHLSLASLQGILQLPPHGFRELPGLQVLDLSGNPKLKWAGAEVFSGLGLLQELDLSGSSLVPLPETLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSPKVVLHCGDTQESARGPDIL

Target ORF information:

RefSeq Version	XM_006229760.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229760.2

ORF Insert Sequence:

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsku ( XM_006229761.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229823.1 Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23136
Clone ID Related Accession (Same CDS sequence)	XM_006229759.3 , XM_006229760.2 , XM_006229761.3
Accession Version	XM_006229761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tsukushin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229761.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229823.1
CDS	348..1409
Translation	MLCTLFLLLLALGTVQTTRPCFPGCQCEEETFGLFDSFSLIRVDCSSLGPHIVPVPIPLDTAHLDLSSNRLETVNESVLGGPGYTTLAGLDLSHNLLTSITPTAFSRLRYLESLDLSHNGLAALPAEVFTSSPLSDINLSHNRLREVSISAFTTHSQGRALHVDLSHNLIHRLLPYPARASLSAPTIQSLNLSWNRLRAVPNLRDLPLRYLSLDGNPLATINPGAFMGLAGLTHLSLASLQGILQLPPHGFRELPGLQVLDLSGNPKLKWAGAEVFSGLGLLQELDLSGSSLVPLPETLLHHLPALQSVSVGQDVQCRRLVREGAYHRQPGSSPKVVLHCGDTQESARGPDIL

Target ORF information:

RefSeq Version	XM_006229761.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tsukushi, small leucine rich proteoglycan (Tsku), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229761.3

ORF Insert Sequence:

ATGTTGTGCA CCCTGTTCCT GCTACTGCTG GCCCTGGGCA CAGTGCAGAC AACTCGGCCA 
TGTTTCCCTG GCTGCCAGTG TGAGGAAGAG ACGTTTGGCC TCTTTGACAG TTTCAGCCTG 
ATCCGAGTGG ACTGCAGCAG CCTGGGCCCC CACATTGTGC CTGTGCCCAT CCCTCTGGAC 
ACAGCCCACC TGGACCTGTC TTCCAACCGG CTAGAAACCG TGAATGAGTC AGTCCTGGGA 
GGGCCAGGCT ATACCACACT GGCTGGCCTG GATCTCAGTC ACAACCTCCT CACCAGCATC 
ACGCCCACTG CCTTCTCCCG CCTTCGCTAC CTGGAGTCAC TGGACCTCAG TCACAATGGC 
CTGGCAGCCC TGCCAGCAGA GGTTTTCACC AGCTCCCCCT TGAGTGATAT CAACCTGAGC 
CATAATCGAC TTAGAGAGGT CTCAATATCT GCCTTCACCA CCCACAGCCA GGGGCGGGCA 
CTGCACGTGG ACCTATCCCA CAATCTTATC CACCGCCTGC TCCCCTATCC AGCCAGGGCC 
AGCCTGTCCG CACCTACCAT TCAGAGCCTG AACCTGTCCT GGAACCGGCT CCGAGCCGTG 
CCCAATCTCC GAGACCTACC CCTGCGTTAC CTGAGCCTGG ATGGGAACCC TCTGGCTACC 
ATCAACCCAG GCGCCTTCAT GGGGCTGGCG GGCCTCACCC ACCTTTCACT GGCAAGCCTA 
CAGGGTATCC TCCAGCTACC ACCCCATGGC TTCCGAGAGC TCCCAGGCCT TCAGGTCCTG 
GACTTGTCTG GTAACCCCAA GCTCAAGTGG GCAGGAGCCG AGGTATTTTC AGGCCTGGGT 
TTGCTGCAAG AACTAGACCT GTCTGGCTCC AGCCTGGTGC CCCTGCCTGA GACGCTGCTA 
CATCACCTCC CTGCTTTACA GAGTGTCAGT GTAGGCCAAG ATGTGCAGTG CCGGCGTCTG 
GTACGGGAGG GTGCCTACCA CCGCCAACCC GGTTCCAGCC CTAAGGTAGT CCTGCACTGT 
GGAGACACCC AGGAATCTGC CAGGGGCCCA GACATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
PloS one7(8)e42516(2012)
Henke A,Grace OC,Ashley GR,Stewart GD,Riddick AC,Yeun H,O'Donnell M,Anderson RA,Thomson AA

Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4.
Proceedings of the National Academy of Sciences of the United States of America108(36)14962-7(2011 Sep)
Ohta K,Ito A,Kuriyama S,Lupo G,Kosaka M,Ohnuma S,Nakagawa S,Tanaka H

Tsukushi is required for anterior commissure formation in mouse brain.
Biochemical and biophysical research communications402(4)813-8(2010 Nov)
Ito A,Shinmyo Y,Abe T,Oshima N,Tanaka H,Ohta K

Identification of a novel rat hepatic gene induced early by insulin, independently of glucose.
The Biochemical journal385(Pt 1)165-71(2005 Jan)
Coffy S,Decaux JF,Girard J,de Keyzer Y,Asfari M

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Tsku cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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