Twnk cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following Twnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa46747 XM_017589287.1
Latest version!
Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
ORa46747 XM_008760430.2
Latest version!
Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
ORa46747 XM_008760431.2
Latest version!
Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
ORa04146 NM_001107599.1
Latest version!
Rattus norvegicus twinkle mtDNA helicase (Twnk), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa46746 XM_008760429.2
Latest version!
Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa46747
    Clone ID Related Accession (Same CDS sequence) XM_008760430.2 , XM_008760431.2 , XM_017589287.1
    Accession Version XM_008760430.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product twinkle protein, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008758652.1
    CDS203..898
    Translation

    Target ORF information:

    RefSeq Version XM_008760430.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008760430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa46747
    Clone ID Related Accession (Same CDS sequence) XM_008760430.2 , XM_008760431.2 , XM_017589287.1
    Accession Version XM_008760431.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product twinkle protein, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008758653.1
    CDS251..946
    Translation

    Target ORF information:

    RefSeq Version XM_008760431.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008760431.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa46747
    Clone ID Related Accession (Same CDS sequence) XM_008760430.2 , XM_008760431.2 , XM_017589287.1
    Accession Version XM_017589287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product twinkle protein, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017444776.1
    CDS238..933
    Translation

    Target ORF information:

    RefSeq Version XM_017589287.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017589287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACTC AGTTTGCTGT GACACGGCTA GAAGAACAAC TGGACAAGTA TGAAGAGTGG 
    GCAGACCGTT TTGAGGACCT GCCTCTCTAT TTCATGACTT TCCACGGACA ACAGAGCATC
    AGGTCTGTCA TAGACACAAT GCAACATGCT GTCTACGTCT ATGACGTTTG TCATGTAGTC
    ATCGACAACT TGCAGTTCAT GATGGGTCAT GAGCAGCTCT CTACTGACAG GATCGCAGCT
    CAAGACTACA TTGTTGGAGC CTTTCGGAAG TTTGCCACAG ACAATAGCTG CCACGTGACT
    CTGGTTATTC ACCCACGGAA AGAAGATGAC GACAAGGAAC TACAGACAGC ATCCATTTTT
    GGCTCAGCCA AAGCCAGCCA AGAAGCAGAC AATGTTCTGA TCTTGCAGGA CAGGAAGCTG
    GTGACTGGGC CTGGGAAACG GTATCTGCAG GTGTCCAAAA ATCGCTTTGA TGGAGACGTA
    GGCGTCTTCC CACTTGAGTT CAACAAGAGT TCCCTTACCT TCTCCATCCC ACCTAAGAGC
    AAAGCCCGAC TCAAGAAGAT TAAGGATGAC AATGGACTAG TGGCCAAGAA GTCCTCCTCT
    GGAAAAAAAG GGTCTGCGCA CCAGAACTCT GAGACTTGCA TAGGCCAGGA CCCCTGCCAG
    CCAGATACCT CCCCGTCCTC AAGGCATAAG AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa04146
    Clone ID Related Accession (Same CDS sequence) NM_001107599.1
    Accession Version NM_001107599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Rattus norvegicus(Norway rat)
    Product twinkle protein, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473986.1. On or before Oct 4, 2007 this sequence version replaced XM_219939.4, XM_001063420.1. ##Evidence-Data-START## Transcript exon combination :: EV770275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTCC TTCTCCGGCG TGCTTATCCC CTCCGGATCT TGCTGCCACT GCGTGGGGAG 
    TGGATGGGTA GGAGAGGCCT GCCCCGAAGC TTGGCTCCAG GCCCTCCCCG AAGAAGGTAC
    AGAAAAGAAG CGCTTCCAGC CTTAGAGGTA CCAGTATCAC CTGTGACTAC AACTGAAATC
    CGCCAATATT TACGAGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGCACC CTCAGACATC AAAAATCAGA AGAAGGATGC TACCACTTCC
    TTCTGCCTCT TCATCGACAA GACCACAGGA CACTTTCTCT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACCTCCA GGCCAGTGTG GAGGGACGAG GCGATGGGGC CAAAGAGGGA
    ATCCTACTTG GGGAGGGCCC AGAAGCTGAG GACAGGGAGG AGGTCTTGAG AATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTGCCTGAT CCCCAGGAAA CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGAGAT TCAATGTCCG CTATCTTCGA
    TCTACTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGTTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATTCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCCCTGA TCGGCCGTAG AGACACTGAG
    GTGGTCCTGA CAAGTCGAGA GCTGGACAGC CTGGCCTTGA GCCAGTCCAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG AACGGTCTGC TTACCCCCAG CCCTACTCCC TTACCTCGAA
    CAGTTCCGGA GGATTGTGTT CTGGCTAGGG GATGACCTTC GATCGTGGGA AGCTGCCAAA
    CTGTTTGCCC GAAAACTGAA CCCCAAGCGA TGCTCTTTGG TGAGGCCTGG GAACCAACAA
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT CCGTACTGCC
    CTCCCTGCCT GGCACAAGTC TATTGTGTCT TTTCGCCAGC TCCGAGAAGA GGTTTTAGGA
    GAGCTATCAA ATGTGGACCA AGCAGCCGGT GTCCGTTGGA GCCGATTCCC AGACCTTAAT
    CGGCTGCTGA AGGGTCATCG GAAGGGCGAG CTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAATGTG AGACTAGCCC GGGTCATGCT GACTCAGTTT
    GCTGTGACAC GGCTAGAAGA ACAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTATTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC TGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTTTGTCATG TAGTCATCGA CAACTTGCAG
    TTCATGATGG GTCATGAGCA GCTCTCTACT GACAGGATCG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC CACAGACAAT AGCTGCCACG TGACTCTGGT TATTCACCCA
    CGGAAAGAAG ATGACGACAA GGAACTACAG ACAGCATCCA TTTTTGGCTC AGCCAAAGCC
    AGCCAAGAAG CAGACAATGT TCTGATCTTG CAGGACAGGA AGCTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAAAATCGC TTTGATGGAG ACGTAGGCGT CTTCCCACTT
    GAGTTCAACA AGAGTTCCCT TACCTTCTCC ATCCCACCTA AGAGCAAAGC CCGACTCAAG
    AAGATTAAGG ATGACAATGG ACTAGTGGCC AAGAAGTCCT CCTCTGGAAA AAAAGGGTCT
    GCGCACCAGA ACTCTGAGAC TTGCATAGGC CAGGACCCCT GCCAGCCAGA TACCTCCCCG
    TCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001101069.1
    CDS713..2764
    Translation

    Target ORF information:

    RefSeq Version NM_001107599.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus twinkle mtDNA helicase (Twnk), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001107599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTCC TTCTCCGGCG TGCTTATCCC CTCCGGATCT TGCTGCCACT GCGTGGGGAG 
    TGGATGGGTA GGAGAGGCCT GCCCCGAAGC TTGGCTCCAG GCCCTCCCCG AAGAAGGTAC
    AGAAAAGAAG CGCTTCCAGC CTTAGAGGTA CCAGTATCAC CTGTGACTAC AACTGAAATC
    CGCCAATATT TACGAGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGCACC CTCAGACATC AAAAATCAGA AGAAGGATGC TACCACTTCC
    TTCTGCCTCT TCATCGACAA GACCACAGGA CACTTTCTCT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACCTCCA GGCCAGTGTG GAGGGACGAG GCGATGGGGC CAAAGAGGGA
    ATCCTACTTG GGGAGGGCCC AGAAGCTGAG GACAGGGAGG AGGTCTTGAG AATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTGCCTGAT CCCCAGGAAA CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGAGAT TCAATGTCCG CTATCTTCGA
    TCTACTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGTTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATTCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCCCTGA TCGGCCGTAG AGACACTGAG
    GTGGTCCTGA CAAGTCGAGA GCTGGACAGC CTGGCCTTGA GCCAGTCCAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG AACGGTCTGC TTACCCCCAG CCCTACTCCC TTACCTCGAA
    CAGTTCCGGA GGATTGTGTT CTGGCTAGGG GATGACCTTC GATCGTGGGA AGCTGCCAAA
    CTGTTTGCCC GAAAACTGAA CCCCAAGCGA TGCTCTTTGG TGAGGCCTGG GAACCAACAA
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT CCGTACTGCC
    CTCCCTGCCT GGCACAAGTC TATTGTGTCT TTTCGCCAGC TCCGAGAAGA GGTTTTAGGA
    GAGCTATCAA ATGTGGACCA AGCAGCCGGT GTCCGTTGGA GCCGATTCCC AGACCTTAAT
    CGGCTGCTGA AGGGTCATCG GAAGGGCGAG CTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAATGTG AGACTAGCCC GGGTCATGCT GACTCAGTTT
    GCTGTGACAC GGCTAGAAGA ACAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTATTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC TGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTTTGTCATG TAGTCATCGA CAACTTGCAG
    TTCATGATGG GTCATGAGCA GCTCTCTACT GACAGGATCG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC CACAGACAAT AGCTGCCACG TGACTCTGGT TATTCACCCA
    CGGAAAGAAG ATGACGACAA GGAACTACAG ACAGCATCCA TTTTTGGCTC AGCCAAAGCC
    AGCCAAGAAG CAGACAATGT TCTGATCTTG CAGGACAGGA AGCTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAAAATCGC TTTGATGGAG ACGTAGGCGT CTTCCCACTT
    GAGTTCAACA AGAGTTCCCT TACCTTCTCC ATCCCACCTA AGAGCAAAGC CCGACTCAAG
    AAGATTAAGG ATGACAATGG ACTAGTGGCC AAGAAGTCCT CCTCTGGAAA AAAAGGGTCT
    GCGCACCAGA ACTCTGAGAC TTGCATAGGC CAGGACCCCT GCCAGCCAGA TACCTCCCCG
    TCCTCAAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa46746
    Clone ID Related Accession (Same CDS sequence) XM_008760429.2
    Accession Version XM_008760429.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product twinkle protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTCC TTCTCCGGCG TGCTTATCCC CTCCGGATCT TGCTGCCACT GCGTGGGGAG 
    TGGATGGGTA GGAGAGGCCT GCCCCGAAGC TTGGCTCCAG GCCCTCCCCG AAGAAGGTAC
    AGAAAAGAAG CGCTTCCAGC CTTAGAGGTA CCAGTATCAC CTGTGACTAC AACTGAAATC
    CGCCAATATT TACGAGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGCACC CTCAGACATC AAAAATCAGA AGAAGGATGC TACCACTTCC
    TTCTGCCTCT TCATCGACAA GACCACAGGA CACTTTCTCT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACCTCCA GGCCAGTGTG GAGGGACGAG GCGATGGGGC CAAAGAGGGA
    ATCCTACTTG GGGAGGGCCC AGAAGCTGAG GACAGGGAGG AGGTCTTGAG AATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTGCCTGAT CCCCAGGAAA CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGAGAT TCAATGTCCG CTATCTTCGA
    TCTACTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGTTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATTCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCCCTGA TCGGCCGTAG AGACACTGAG
    GTGGTCCTGA CAAGTCGAGA GCTGGACAGC CTGGCCTTGA GCCAGTCCAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG AACGGTCTGC TTACCCCCAG CCCTACTCCC TTACCTCGAA
    CAGTTCCGGA GGATTGTGTT CTGGCTAGGG GATGACCTTC GATCGTGGGA AGCTGCCAAA
    CTGTTTGCCC GAAAACTGAA CCCCAAGCGA TGCTCTTTGG TGAGGCCTGG GAACCAACAA
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT CCGTACTGCC
    CTCCCTGCCT GGCACAAGTC TATTGTGTCT TTTCGCCAGC TCCGAGAAGA GGTTTTAGGA
    GAGCTATCAA ATGTGGACCA AGCAGCCGGT GTCCGTTGGA GCCGATTCCC AGACCTTAAT
    CGGCTGCTGA AGGGTCATCG GAAGGGCGAG CTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAATGTG AGACTAGCCC GGGTCATGCT GACTCAGTTT
    GCTGTGACAC GGCTAGAAGA ACAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTATTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC TGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTTTGTCATG TAGTCATCGA CAACTTGCAG
    TTCATGATGG GTCATGAGCA GCTCTCTACT GACAGGATCG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC CACAGACAAT AGCTGCCACG TGACTCTGGT TATTCACCCA
    CGGAAAGAAG ATGACGACAA GGAACTACAG ACAGCATCCA TTTTTGGCTC AGCCAAAGCC
    AGCCAAGAAG CAGACAATGT TCTGATCTTG CAGGACAGGA AGCTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAAAATCGC TTTGATGGAG ACGTAGGCGT CTTCCCACTT
    GAGTTCAACA AGAGTTCCCT TACCTTCTCC ATCCCACCTA AGAGCAAAGC CCGACTCAAG
    AAGATTAAGG ATGACAATGG ACTAGTGGCC AAGAAGTCCT CCTCTGGAAA AAAAGGGTCT
    GCGCACCAGA ACTCTGAGAC TTGCATAGGC CAGGACCCCT GCCAGCCAGA TACCTCCCCG
    TCCTCAAGGC ATAAGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008758651.1
    CDS769..2829
    Translation

    Target ORF information:

    RefSeq Version XM_008760429.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus progressive external ophthalmoplegia 1 (Peo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008760429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGCTCC TTCTCCGGCG TGCTTATCCC CTCCGGATCT TGCTGCCACT GCGTGGGGAG 
    TGGATGGGTA GGAGAGGCCT GCCCCGAAGC TTGGCTCCAG GCCCTCCCCG AAGAAGGTAC
    AGAAAAGAAG CGCTTCCAGC CTTAGAGGTA CCAGTATCAC CTGTGACTAC AACTGAAATC
    CGCCAATATT TACGAGCACA TGGGATCCCC TTCCAGGATG GCCACAGCTG CCTTCGAGCA
    CCAAGTCCCT TTGTGGCACC CTCAGACATC AAAAATCAGA AGAAGGATGC TACCACTTCC
    TTCTGCCTCT TCATCGACAA GACCACAGGA CACTTTCTCT GCATGACCAG CCTAGCAGAA
    GGGAGCTGGG AAGACCTCCA GGCCAGTGTG GAGGGACGAG GCGATGGGGC CAAAGAGGGA
    ATCCTACTTG GGGAGGGCCC AGAAGCTGAG GACAGGGAGG AGGTCTTGAG AATCTGGAAC
    CGAGCCATAC CTCTTTGGGA GCTGCCTGAT CCCCAGGAAA CCCAGCTAGC TCGTGTGATG
    TTTGGCCTTA CCAAGGTGAC AGATGACACA CTCAGGAGAT TCAATGTCCG CTATCTTCGA
    TCTACTCGAA GTCTGGTCTT CCCTTGGTTC ACCCCTGGGA GCTCAGGATT ACGTGGCCTG
    AAGCTACTAG GGGTTGAGGG TCAGGAAAAT GGAGTACAGT ATGTAGAGAC CACCATTCCC
    CGGCCTGGTG TCTATCACAA CCTATTTGGA TTACCCCTGA TCGGCCGTAG AGACACTGAG
    GTGGTCCTGA CAAGTCGAGA GCTGGACAGC CTGGCCTTGA GCCAGTCCAC GGGGCTGCCA
    ACCCTTTCCC TACCCCGAGG AACGGTCTGC TTACCCCCAG CCCTACTCCC TTACCTCGAA
    CAGTTCCGGA GGATTGTGTT CTGGCTAGGG GATGACCTTC GATCGTGGGA AGCTGCCAAA
    CTGTTTGCCC GAAAACTGAA CCCCAAGCGA TGCTCTTTGG TGAGGCCTGG GAACCAACAA
    CCTCGTCCCC TGGAGGCCTT AAACCAAGGC TTAAGTCTTC CCCGTATTCT CCGTACTGCC
    CTCCCTGCCT GGCACAAGTC TATTGTGTCT TTTCGCCAGC TCCGAGAAGA GGTTTTAGGA
    GAGCTATCAA ATGTGGACCA AGCAGCCGGT GTCCGTTGGA GCCGATTCCC AGACCTTAAT
    CGGCTGCTGA AGGGTCATCG GAAGGGCGAG CTGACGGTCT TTACAGGGCC AACAGGCAGT
    GGCAAGACAA CATTCATCAG TGAGTATGCC CTGGACTTGT GTACCCAGGG AGTGAACACA
    CTGTGGGGTA GCTTTGAGAT CAGCAATGTG AGACTAGCCC GGGTCATGCT GACTCAGTTT
    GCTGTGACAC GGCTAGAAGA ACAACTGGAC AAGTATGAAG AGTGGGCAGA CCGTTTTGAG
    GACCTGCCTC TCTATTTCAT GACTTTCCAC GGACAACAGA GCATCAGGTC TGTCATAGAC
    ACAATGCAAC ATGCTGTCTA CGTCTATGAC GTTTGTCATG TAGTCATCGA CAACTTGCAG
    TTCATGATGG GTCATGAGCA GCTCTCTACT GACAGGATCG CAGCTCAAGA CTACATTGTT
    GGAGCCTTTC GGAAGTTTGC CACAGACAAT AGCTGCCACG TGACTCTGGT TATTCACCCA
    CGGAAAGAAG ATGACGACAA GGAACTACAG ACAGCATCCA TTTTTGGCTC AGCCAAAGCC
    AGCCAAGAAG CAGACAATGT TCTGATCTTG CAGGACAGGA AGCTGGTGAC TGGGCCTGGG
    AAACGGTATC TGCAGGTGTC CAAAAATCGC TTTGATGGAG ACGTAGGCGT CTTCCCACTT
    GAGTTCAACA AGAGTTCCCT TACCTTCTCC ATCCCACCTA AGAGCAAAGC CCGACTCAAG
    AAGATTAAGG ATGACAATGG ACTAGTGGCC AAGAAGTCCT CCTCTGGAAA AAAAGGGTCT
    GCGCACCAGA ACTCTGAGAC TTGCATAGGC CAGGACCCCT GCCAGCCAGA TACCTCCCCG
    TCCTCAAGGC ATAAGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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