Rc3h2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Rc3h2
Entrez Gene ID	311909
Full Name	ring finger and CCCH-type domains 2
Synonyms	Mnab
General protein information	Preferred Names ring finger and CCCH-type domains 2 Names roquin-2 RING finger and CCCH-type zinc finger domain-containing protein 2 membrane associated DNA binding protein ring finger and CCCH-type zinc finger domains 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761754.2	XP_008759976.1	roquin-2 isoform X2
NM_001107839.1	NP_001101309.1	roquin-2
XM_017591850.1	XP_017447339.1	roquin-2 isoform X1
XM_008761755.2	XP_008759977.1	roquin-2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RC3H2	XP_520240.3
Canis lupus familiaris (dog)	RC3H2	XP_003639362.1
Rattus norvegicus (Norway rat)	Rc3h2	NP_001101309.1
	RC3H2	XP_001086022.2
Homo sapiens (human)	RC3H2	NP_001094058.1
Bos taurus (cattle)	RC3H2	XP_002691620.3
Gallus gallus (chicken)	RC3H2	XP_415394.2
Mus musculus (house mouse)	Rc3h2	NP_001094061.1
Danio rerio (zebrafish)	rc3h2	XP_005155680.1

Gene Ontology
Bibliography

Related articles in PubMed

New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang Q, Fan L, Hou F, Dong A, Wang YX, Tong Y
Scientific reports515660(2015 Oct)

Structure of human Roquin-2 and its complex with constitutive-decay element RNA.
Sakurai S, Ohto U, Shimizu T
Acta crystallographica. Section F, Structural biology communications71(Pt 8)1048-54(2015 Aug)

Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.
Srivastava M, Duan G, Kershaw NJ, Athanasopoulos V, Yeo JH, Ose T, Hu D, Brown SH, Jergic S, Patel HR, Pratama A, Richards S, Verma A, Jones EY, Heissmeyer V, Preiss T, Dixon NE, Chong MM, Babon JJ, Vinuesa CG
Nature communications66253(2015 Feb)

Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote T(H)17 differentiation.
Jeltsch KM, Hu D, Brenner S, Z?ller J, Heinz GA, Nagel D, Vogel KU, Rehage N, Warth SC, Edelmann SL, Gloury R, Martin N, Lohs C, Lech M, Stehklein JE, Geerlof A, Kremmer E, Weber A, Anders HJ, Schmitz I, Schmidt-Supprian M, Fu M, Holtmann H, Krappmann D, Ruland J, Kallies A, Heikenwalder M, Heissmeyer V
Nature immunology15(11)1079-89(2014 Nov)

Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to regulate stress responses.
Maruyama T, Araki T, Kawarazaki Y, Naguro I, Heynen S, Aza-Blanc P, Ronai Z, Matsuzawa A, Ichijo H
Science signaling7(309)ra8(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rc3h2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rc3h2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06106	NM_001107839.1 Latest version!	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa06106	XM_017591850.1 Latest version!	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa25799	XM_008761754.2 Latest version!	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa47098	XM_008761755.2 Latest version!	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rc3h2 ( NM_001107839.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101309.1 Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), mRNA.

Price & Availability↑

CloneID	ORa06106
Clone ID Related Accession (Same CDS sequence)	NM_001107839.1 , XM_017591850.1
Accession Version	NM_001107839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	roquin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473983.2. On or before Oct 4, 2007 this sequence version replaced XM_001053668.1, XM_001053333.1, XM_001053613.1, XM_231249.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCAGTAACAT CTTGCTTTAG CCAGCCACGC 
CCAGTGTCTA TTAGCAGTGC AAGCTGCCTC CCTATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GAGAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101309.1
CDS	192..3755
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVAVNSTVTTTAGNVISVIGSTETTGKIVPSTNGISNTESSVSQLIPRSADSAGRTLETVKKVGKVGTNGQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSENIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSNNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVTCQPPNDPRTTVPLPREPCGHLKSSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESGSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSLELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPVQQHQKDPVKQKRQSLGEDHVILEEQKPILPVTSCFSQPRPVSISSASCLPITTSVSVGNLILKTHVMSEEKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	NM_001107839.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107839.1

ORF Insert Sequence:

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCAGTAACAT CTTGCTTTAG CCAGCCACGC 
CCAGTGTCTA TTAGCAGTGC AAGCTGCCTC CCTATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GAGAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( XM_017591850.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447339.1 Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06106
Clone ID Related Accession (Same CDS sequence)	NM_001107839.1 , XM_017591850.1
Accession Version	XM_017591850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	roquin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCAGTAACAT CTTGCTTTAG CCAGCCACGC 
CCAGTGTCTA TTAGCAGTGC AAGCTGCCTC CCTATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GAGAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447339.1
CDS	521..4084
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVAVNSTVTTTAGNVISVIGSTETTGKIVPSTNGISNTESSVSQLIPRSADSAGRTLETVKKVGKVGTNGQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSENIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSNNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVTCQPPNDPRTTVPLPREPCGHLKSSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESGSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSLELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPVQQHQKDPVKQKRQSLGEDHVILEEQKPILPVTSCFSQPRPVSISSASCLPITTSVSVGNLILKTHVMSEEKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	XM_017591850.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591850.1

ORF Insert Sequence:

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG TGAAGACCAT 
GTGATTCTGG AGGAGCAAAA ACCAATTCTG CCAGTAACAT CTTGCTTTAG CCAGCCACGC 
CCAGTGTCTA TTAGCAGTGC AAGCTGCCTC CCTATCACCA CATCTGTCAG TGTTGGCAAC 
CTCATTCTGA AAACTCACGT TATGTCTGAA GAGAAAAACG ACTTTTTAAA ACCTATTGCA 
AATGGGAAGA TGGTTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( XM_008761754.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759976.1 Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X2, mRNA.

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CloneID	ORa25799
Clone ID Related Accession (Same CDS sequence)	XM_008761754.2
Accession Version	XM_008761754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	roquin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG GAGGAGCAAA 
AACCAATTCT GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759976.1
CDS	302..3679
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKMSATVRTFPLLNKVAVNSTVTTTAGNVISVIGSTETTGKIVPSTNGISNTESSVSQLIPRSADSAGRTLETVKKVGKVGTNGQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSENIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSNNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVTCQPPNDPRTTVPLPREPCGHLKSSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESGSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSLELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPVQQHQKDPVKQKRQSLGRSKNQFCQ

Target ORF information:

RefSeq Version	XM_008761754.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761754.2

ORF Insert Sequence:

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAAGTACC GATTAAGGAA CAAAAAGATG AGCGCGACTG TAAGAACATT TCCTCTTCTG 
AATAAAGTTG CTGTAAACAG CACTGTCACA ACCACAGCCG GAAATGTCAT TTCTGTCATA 
GGAAGTACTG AAACAACTGG GAAAATTGTT CCAAGTACAA ATGGAATTTC AAATACAGAA 
AGCAGTGTTT CCCAGCTAAT CCCACGAAGT GCTGACAGTG CAGGAAGGAC TTTGGAGACT 
GTGAAGAAAG TTGGGAAAGT TGGCACTAAT GGTCAGAATG CTGGGCCCTC TGCAGAGTCT 
GTGTCTGAAA ATAAAATTGG TTCTCCACCC AAGACTCCTG TAAGTAATGC AGCAGCTACC 
TCCGCTGGGC CCTCTAATTT TGGAACAGAG CTGAATTCCC TGCCTCCCAA ATCCAGCCCA 
TTTCTAACTC GAGTTCCAGT ATATCCTCAG CATTCTGAAA ACATCCAGTA TTTTCAAGAT 
CCAAGGACTC AGATACCTTT TGAAGTCCCA CAATACCCAC AAACAGGATA CTACCCACCA 
CCTCCAACGG TACCAGCTGG TGTGACTCCC TGTGTTCCTC GCTTTGTGAG GTCCAATAAT 
GTTCCAGAGT CCTCCCTCCC ACCTGCTTCC ATGCCATATG CTGATCATTA CAGTACATTT 
TCCCCTCGAG ATCGAATGAA TTCTTCTCCT TACCAACCTC CTCCTCCGCA GCAGTATGGA 
CCAGTTCCTC CAGTACCCTC TGGGATGTAT GCTCCTGTGT ATGACAGCAG GCGCATCTGG 
CGCCCAGCTA TGTACCAGCG AGATGACATT ATTAGAAGCA ATTCTTTACC TCCAATGGAT 
GTGATGCACT CATCTGTCTA TCAGACATCT TTACGGGAAA GATACAACTC ATTAGATGGT 
TATTATTCAG TGACTTGTCA GCCACCAAAT GACCCAAGGA CAACTGTGCC TTTACCAAGG 
GAACCTTGTG GTCATTTAAA AAGCAGTTGT GAGGAACAGT TAAGAAGAAA GCCAGATCAG 
TGGACACAGT ATCATACCCA GAAGACACCT GTCTCTTCAA CTCTTCCTGT GGCAACACAG 
TCACCAACAC CACCTTCTCC TTTGTTCAGT GTAGACTTCA GATCGGATTT CTCTGAGAGT 
GGGAGTGGCA CAAAATTTGA GGAAGACCAT CTTTCCCATT ATTCTCCTTG GTCTTGTGGC 
ACTATAGGCT CTTGTATAAA TGCCATTGAT TCAGAGCCCA AAGATGTAAT TGCCAATTCA 
AATGCTGTGC TGATGGACCT GGACAGTGGT GATGTAAAGA GAAGAGTGCA TTTATTTGAA 
GCTCAGAGAA GGACAAAAGA AGAAGACCCA ATTATTCCCT TTAGTGACGG ACCCATTATC 
TCAAAATGGG GTGCAATTTC CCGATCCTCC CGAACAGGTT ACCATACAAC AGATCCCGTC 
CAGGCAACTG CTTCCCAAGG AAGTGCAACT AAGCCCATCA GTGTATCAGA TTATGTCCCT 
TATGTTAATG CTGTCGATTC AAGGTGGAGT TCATATGGTA ACGATGCCAC ATCATCAGCA 
CACTATATTG AACGGGACAG ATTCATTGTT ACCGATTTAT CTGGTCATAG AAAGCACTCC 
AGTACCGGAG ACCTCTTGAG CCTTGAGCTT CAGCAGGCCA AGAGTAACTC ATTACTTCTT 
CAGAGGGAAG CTAATGCTCT GGCCATGCAA CAAAAGTGGA ATTCCCTGGA TGAAGGCCGT 
CATCTTACTT TAAATCTCCT AAGCAAGGAA ATTGAACTGA GAAATGGAGA GAATGATTAC 
ACAGAAGACA CTGTAGATAC CAAGCCTGAT AGAGATATTG AGTTAGAACT TTCAGCCCTT 
GATACTGATG AGCCTGATGG CCAAAGTGAA CAAATTGAAG AAATTCTGGA CATACAACTT 
GGTATCAGTT CTCAAAATGA TCAGTTGCTA AATGGAACTG CAGTGGAAAA TGGTCATCCT 
GTCCAGCAGC ACCAAAAGGA CCCCGTAAAG CAGAAGAGAC AGAGTTTAGG GAGGAGCAAA 
AACCAATTCT GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rc3h2 ( XM_008761755.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759977.1 Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X3, mRNA.

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CloneID	ORa47098
Clone ID Related Accession (Same CDS sequence)	XM_008761755.2
Accession Version	XM_008761755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	roquin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAATAAAA TTGGTTCTCC ACCCAAGACT CCTGTAAGTA ATGCAGCAGC TACCTCCGCT 
GGGCCCTCTA ATTTTGGAAC AGAGCTGAAT TCCCTGCCTC CCAAATCCAG CCCATTTCTA 
ACTCGAGTTC CAGTATATCC TCAGCATTCT GAAAACATCC AGTATTTTCA AGATCCAAGG 
ACTCAGATAC CTTTTGAAGT CCCACAATAC CCACAAACAG GATACTACCC ACCACCTCCA 
ACGGTACCAG CTGGTGTGAC TCCCTGTGTT CCTCGCTTTG TGAGGTCCAA TAATGTTCCA 
GAGTCCTCCC TCCCACCTGC TTCCATGCCA TATGCTGATC ATTACAGTAC ATTTTCCCCT 
CGAGATCGAA TGAATTCTTC TCCTTACCAA CCTCCTCCTC CGCAGCAGTA TGGACCAGTT 
CCTCCAGTAC CCTCTGGGAT GTATGCTCCT GTGTATGACA GCAGGCGCAT CTGGCGCCCA 
GCTATGTACC AGCGAGATGA CATTATTAGA AGCAATTCTT TACCTCCAAT GGATGTGATG 
CACTCATCTG TCTATCAGAC ATCTTTACGG GAAAGATACA ACTCATTAGA TGGTTATTAT 
TCAGTGACTT GTCAGCCACC AAATGACCCA AGGACAACTG TGCCTTTACC AAGGGAACCT 
TGTGGTCATT TAAAAAGCAG TTGTGAGGAA CAGTTAAGAA GAAAGCCAGA TCAGTGGACA 
CAGTATCATA CCCAGAAGAC ACCTGTCTCT TCAACTCTTC CTGTGGCAAC ACAGTCACCA 
ACACCACCTT CTCCTTTGTT CAGTGTAGAC TTCAGATCGG ATTTCTCTGA GAGTGGGAGT 
GGCACAAAAT TTGAGGAAGA CCATCTTTCC CATTATTCTC CTTGGTCTTG TGGCACTATA 
GGCTCTTGTA TAAATGCCAT TGATTCAGAG CCCAAAGATG TAATTGCCAA TTCAAATGCT 
GTGCTGATGG ACCTGGACAG TGGTGATGTA AAGAGAAGAG TGCATTTATT TGAAGCTCAG 
AGAAGGACAA AAGAAGAAGA CCCAATTATT CCCTTTAGTG ACGGACCCAT TATCTCAAAA 
TGGGGTGCAA TTTCCCGATC CTCCCGAACA GGTTACCATA CAACAGATCC CGTCCAGGCA 
ACTGCTTCCC AAGGAAGTGC AACTAAGCCC ATCAGTGTAT CAGATTATGT CCCTTATGTT 
AATGCTGTCG ATTCAAGGTG GAGTTCATAT GGTAACGATG CCACATCATC AGCACACTAT 
ATTGAACGGG ACAGATTCAT TGTTACCGAT TTATCTGGTC ATAGAAAGCA CTCCAGTACC 
GGAGACCTCT TGAGCCTTGA GCTTCAGCAG GCCAAGAGTA ACTCATTACT TCTTCAGAGG 
GAAGCTAATG CTCTGGCCAT GCAACAAAAG TGGAATTCCC TGGATGAAGG CCGTCATCTT 
ACTTTAAATC TCCTAAGCAA GGAAATTGAA CTGAGAAATG GAGAGAATGA TTACACAGAA 
GACACTGTAG ATACCAAGCC TGATAGAGAT ATTGAGTTAG AACTTTCAGC CCTTGATACT 
GATGAGCCTG ATGGCCAAAG TGAACAAATT GAAGAAATTC TGGACATACA ACTTGGTATC 
AGTTCTCAAA ATGATCAGTT GCTAAATGGA ACTGCAGTGG AAAATGGTCA TCCTGTCCAG 
CAGCACCAAA AGGACCCCGT AAAGCAGAAG AGACAGAGTT TAGGTGAAGA CCATGTGATT 
CTGGAGGAGC AAAAACCAAT TCTGCCAGTA ACATCTTGCT TTAGCCAGCC ACGCCCAGTG 
TCTATTAGCA GTGCAAGCTG CCTCCCTATC ACCACATCTG TCAGTGTTGG CAACCTCATT 
CTGAAAACTC ACGTTATGTC TGAAGAGAAA AACGACTTTT TAAAACCTAT TGCAAATGGG 
AAGATGGTTA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759977.1
CDS	302..3559
Translation	MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCVEDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFIQNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELENKIGSPPKTPVSNAAATSAGPSNFGTELNSLPPKSSPFLTRVPVYPQHSENIQYFQDPRTQIPFEVPQYPQTGYYPPPPTVPAGVTPCVPRFVRSNNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPVPPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVTCQPPNDPRTTVPLPREPCGHLKSSCEEQLRRKPDQWTQYHTQKTPVSSTLPVATQSPTPPSPLFSVDFRSDFSESGSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDVIANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTDLSGHRKHSSTGDLLSLELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKEIELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQLLNGTAVENGHPVQQHQKDPVKQKRQSLGEDHVILEEQKPILPVTSCFSQPRPVSISSASCLPITTSVSVGNLILKTHVMSEEKNDFLKPIANGKMVNS

Target ORF information:

RefSeq Version	XM_008761755.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and CCCH-type domains 2 (Rc3h2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761755.2

ORF Insert Sequence:

ATGCCTGTGC AGGCCGCTCA ATGGACAGAG TTTCTGTCCT GTCCAATCTG CTATAATGAA 
TTTGATGAGA ATGTGCACAA ACCCATCAGT TTAGGTTGTT CACACACTGT TTGCAAGACC 
TGCTTGAATA AACTTCACCG AAAAGCGTGT CCTTTTGACC AGACTGCCAT AAACACAGAC 
ATTGATGTGC TCCCTGTCAA CTTTGCACTT CTTCAGTTAG TTGGAGCCCA GGTACCAGAT 
CATCAGTCAA TAAAGTTAAG TAATCTAGGT GAGAATAAAC ACTATGAAGT GGCAAAAAAA 
TGTGTGGAGG ATTTGGCACT CTACTTAAAA CCACTAAGTG GAGGTAAAGG TGTAGCTAGT 
TTGAACCAGA GTGCCCTGAG CCGTCCAATG CAAAGGAAAC TAGTTACACT TGTAAACTGT 
CAACTGGTGG AGGAAGAAGG TCGTGTCAGA GCTATGCGAG CGGCCCGTTC ACTAGGAGAA 
AGAACTGTGA CAGAACTGAT ACTGCAGCAC CAGAACCCTC AGCAGCTGTC TGCCAACCTG 
TGGGCTGCTG TCAGGGCTCG AGGATGCCAG TTTCTAGGGC CAGCTATGCA AGAAGAAGCC 
TTGAAGTTGG TATTGCTGGC ATTAGAAGAT GGTTCTGCTC TCTCTAGGAA AGTTCTGGTA 
CTTTTTGTTG TGCAAAGACT AGAACCAAGA TTTCCTCAGG CATCAAAAAC AAGTATTGGC 
CATGTTGTGC AGCTACTGTA CCGAGCTTCT TGTTTTAAGG TTACTAAAAG GGATGAAGAC 
TCTTCCCTGA TGCAGCTGAA GGAGGAATTT CGGAGTTATG AGGCACTACG CAGAGAACAT 
GATGCCCAAA TTGTTCATAT TGCCATGGAA GCAGGACTCC GTATTTCACC TGAGCAGTGG 
TCTTCACTGC TATATGGGGA CTTGGCTCAT AAATCACACA TGCAGTCTAT CATTGATAAG 
CTACAGTCTC CAGAATCATT TGCAAAGAGT GTCCAAGAAT TGACAATTGT TTTGCAACGA 
ACAGGTGACC CCGCTAATTT AAATAGACTG AGGCCTCATT TAGAGCTTCT TGCAAACATA 
GATCCTAATC CAGATGCTGT TTCACCAACT TGGGAGCAAC TGGAAAATGC AATGGTAGCT 
GTTAAAACAG TGGTTCATGG TCTTGTGGAC TTCATACAAA ATTATAGTAG GAAAGGTCAT 
GAGACACCCC AGCCACAGCC AAACAGCAAG TATAAAACTA GTATGTGCCG AGATTTACGA 
CAACAAGGGG GTTGTCCACG AGGAACAAAT TGTACATTTG CCCATTCTCA GGAAGAGCTT 
GAAAATAAAA TTGGTTCTCC ACCCAAGACT CCTGTAAGTA ATGCAGCAGC TACCTCCGCT 
GGGCCCTCTA ATTTTGGAAC AGAGCTGAAT TCCCTGCCTC CCAAATCCAG CCCATTTCTA 
ACTCGAGTTC CAGTATATCC TCAGCATTCT GAAAACATCC AGTATTTTCA AGATCCAAGG 
ACTCAGATAC CTTTTGAAGT CCCACAATAC CCACAAACAG GATACTACCC ACCACCTCCA 
ACGGTACCAG CTGGTGTGAC TCCCTGTGTT CCTCGCTTTG TGAGGTCCAA TAATGTTCCA 
GAGTCCTCCC TCCCACCTGC TTCCATGCCA TATGCTGATC ATTACAGTAC ATTTTCCCCT 
CGAGATCGAA TGAATTCTTC TCCTTACCAA CCTCCTCCTC CGCAGCAGTA TGGACCAGTT 
CCTCCAGTAC CCTCTGGGAT GTATGCTCCT GTGTATGACA GCAGGCGCAT CTGGCGCCCA 
GCTATGTACC AGCGAGATGA CATTATTAGA AGCAATTCTT TACCTCCAAT GGATGTGATG 
CACTCATCTG TCTATCAGAC ATCTTTACGG GAAAGATACA ACTCATTAGA TGGTTATTAT 
TCAGTGACTT GTCAGCCACC AAATGACCCA AGGACAACTG TGCCTTTACC AAGGGAACCT 
TGTGGTCATT TAAAAAGCAG TTGTGAGGAA CAGTTAAGAA GAAAGCCAGA TCAGTGGACA 
CAGTATCATA CCCAGAAGAC ACCTGTCTCT TCAACTCTTC CTGTGGCAAC ACAGTCACCA 
ACACCACCTT CTCCTTTGTT CAGTGTAGAC TTCAGATCGG ATTTCTCTGA GAGTGGGAGT 
GGCACAAAAT TTGAGGAAGA CCATCTTTCC CATTATTCTC CTTGGTCTTG TGGCACTATA 
GGCTCTTGTA TAAATGCCAT TGATTCAGAG CCCAAAGATG TAATTGCCAA TTCAAATGCT 
GTGCTGATGG ACCTGGACAG TGGTGATGTA AAGAGAAGAG TGCATTTATT TGAAGCTCAG 
AGAAGGACAA AAGAAGAAGA CCCAATTATT CCCTTTAGTG ACGGACCCAT TATCTCAAAA 
TGGGGTGCAA TTTCCCGATC CTCCCGAACA GGTTACCATA CAACAGATCC CGTCCAGGCA 
ACTGCTTCCC AAGGAAGTGC AACTAAGCCC ATCAGTGTAT CAGATTATGT CCCTTATGTT 
AATGCTGTCG ATTCAAGGTG GAGTTCATAT GGTAACGATG CCACATCATC AGCACACTAT 
ATTGAACGGG ACAGATTCAT TGTTACCGAT TTATCTGGTC ATAGAAAGCA CTCCAGTACC 
GGAGACCTCT TGAGCCTTGA GCTTCAGCAG GCCAAGAGTA ACTCATTACT TCTTCAGAGG 
GAAGCTAATG CTCTGGCCAT GCAACAAAAG TGGAATTCCC TGGATGAAGG CCGTCATCTT 
ACTTTAAATC TCCTAAGCAA GGAAATTGAA CTGAGAAATG GAGAGAATGA TTACACAGAA 
GACACTGTAG ATACCAAGCC TGATAGAGAT ATTGAGTTAG AACTTTCAGC CCTTGATACT 
GATGAGCCTG ATGGCCAAAG TGAACAAATT GAAGAAATTC TGGACATACA ACTTGGTATC 
AGTTCTCAAA ATGATCAGTT GCTAAATGGA ACTGCAGTGG AAAATGGTCA TCCTGTCCAG 
CAGCACCAAA AGGACCCCGT AAAGCAGAAG AGACAGAGTT TAGGTGAAGA CCATGTGATT 
CTGGAGGAGC AAAAACCAAT TCTGCCAGTA ACATCTTGCT TTAGCCAGCC ACGCCCAGTG 
TCTATTAGCA GTGCAAGCTG CCTCCCTATC ACCACATCTG TCAGTGTTGG CAACCTCATT 
CTGAAAACTC ACGTTATGTC TGAAGAGAAA AACGACTTTT TAAAACCTAT TGCAAATGGG 
AAGATGGTTA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Scientific reports515660(2015 Oct)
Zhang Q,Fan L,Hou F,Dong A,Wang YX,Tong Y

Structure of human Roquin-2 and its complex with constitutive-decay element RNA.
Acta crystallographica. Section F, Structural biology communications71(Pt 8)1048-54(2015 Aug)
Sakurai S,Ohto U,Shimizu T

Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.
Nature communications66253(2015 Feb)
Srivastava M,Duan G,Kershaw NJ,Athanasopoulos V,Yeo JH,Ose T,Hu D,Brown SH,Jergic S,Patel HR,Pratama A,Richards S,Verma A,Jones EY,Heissmeyer V,Preiss T,Dixon NE,Chong MM,Babon JJ,Vinuesa CG

Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote T(H)17 differentiation.
Nature immunology15(11)1079-89(2014 Nov)
Jeltsch KM,Hu D,Brenner S,Z?ller J,Heinz GA,Nagel D,Vogel KU,Rehage N,Warth SC,Edelmann SL,Gloury R,Martin N,Lohs C,Lech M,Stehklein JE,Geerlof A,Kremmer E,Weber A,Anders HJ,Schmitz I,Schmidt-Supprian M,Fu M,Holtmann H,Krappmann D,Ruland J,Kallies A,Heikenwalder M,Heissmeyer V

Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to regulate stress responses.
Science signaling7(309)ra8(2014 Jan)
Maruyama T,Araki T,Kawarazaki Y,Naguro I,Heynen S,Aza-Blanc P,Ronai Z,Matsuzawa A,Ichijo H

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Rc3h2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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