Usp28 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Usp28
Entrez Gene ID	315639
Full Name	ubiquitin specific peptidase 28
General protein information	Preferred Names ubiquitin specific peptidase 28 Names ubiquitin carboxyl-terminal hydrolase 28 deubiquitinating enzyme 28 ubiquitin specific protease 28 ubiquitin thioesterase 28 ubiquitin thiolesterase 28 ubiquitin-specific-processing protease 28
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q23

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243007.3	XP_006243069.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
XM_006243008.3	XP_006243070.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_006243009.3	XP_006243071.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
XM_006243010.3	XP_006243072.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
XM_006243011.3	XP_006243073.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
XM_006243006.3	XP_006243068.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
NM_001108144.1	NP_001101614.1	ubiquitin carboxyl-terminal hydrolase 28
XM_006243012.3	XP_006243074.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X7

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Usp28	NP_001101614.1
Danio rerio (zebrafish)	usp28	XP_001920096.1
Xenopus tropicalis (tropical clawed frog)	usp28	XP_004916150.1
Canis lupus familiaris (dog)	USP28	XP_005619813.1
Mus musculus (house mouse)	Usp28	NP_780691.2
Pan troglodytes (chimpanzee)	USP28	XP_003313357.2
	USP28	XP_002799842.1
Bos taurus (cattle)	USP28	NP_001179927.1
Homo sapiens (human)	USP28	NP_065937.1
Gallus gallus (chicken)	USP28	NP_001026667.1

Gene Ontology
Bibliography

Related articles in PubMed

A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
Serra RW, Fang M, Park SM, Hutchinson L, Green MR
eLife3e02313(2014 Mar)

The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint.
Bassermann F, Frescas D, Guardavaccaro D, Busino L, Peschiaroli A, Pagano M
Cell134(2)256-67(2008 Jul)

Fbw7 and Usp28 regulate myc protein stability in response to DNA damage.
Popov N, Herold S, Llamazares M, Sch?lein C, Eilers M
Cell cycle (Georgetown, Tex.)6(19)2327-31(2007 Oct)

The ubiquitin-specific protease USP28 is required for MYC stability.
Popov N, Wanzel M, Madiredjo M, Zhang D, Beijersbergen R, Bernards R, Moll R, Elledge SJ, Eilers M
Nature cell biology9(7)765-74(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usp28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06741	NM_001108144.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31125	XM_006243006.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31126	XM_006243007.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31127	XM_006243008.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31128	XM_006243009.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31129	XM_006243010.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31130	XM_006243011.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa31131	XM_006243012.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp28 ( NM_001108144.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101614.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), mRNA.

Price & Availability↑

CloneID	ORa06741
Clone ID Related Accession (Same CDS sequence)	NM_001108144.1
Accession Version	NM_001108144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473975.1. On or before Oct 4, 2007 this sequence version replaced XM_001070037.1, XM_236240.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTTGCCT 
GAATTTCGAA GACTTGTTCT CAGTTACAGC CTGCCACAGA ACATTCTTGA AAATTGTCGA 
AGTCACACTG AAAAAAGAAA TATCATGTTT ATGCAAGAGC TTCAGTATTT ATTTGCTCTG 
TTATTGGGAT CAAATCGGAA ATTTGTAGAT CCTTCTGCTG CCCTGGATCT ACTGAAGGGG 
GCCTTCCGAT CCTCAGAGGA GCAACAGCAA GATGTGAGTG AATTCACACA CAAGCTCCTG 
GATTGGCTGG AGGATGCGTT CCAGCTAGCT GTTAATGTTA ACAGCAATCC CAGGACTAAA 
TCTGAAAACC CCATGGTGCA GCTGTTCTAC GGTACCTTCC TGACAGAAGG GGTCCGAGAA 
GGAAAACCCT TTTGTAACAA CGAGACCTTC GGCCAGTACC CTCTTCAGGT AAACGGTTAC 
CACAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAAG GTGACATTGC GCTGCTTCCT 
TCTGATCGCT CAGTGAAATA CGGACAAGAG CGTTGGTTTA CAAAGCTGCC TCCAGTGTTG 
ACCTTCGAAC TCTCCAGATT TGAGTTTAAT CAGTCTCTTG GGCAGCCTGA GAAAATTCAT 
AATAAGCTGG AATTTCCTCA GGTTATTTAT ATGGATAGGT ACATGTACAA AAGCAAAGAG 
CTTATCCGAA GTAAGAGAGA GAGCATTCGA AAGTTGAAGG AGGAAATACA AGTTCTGCAG 
CAGAAATTAG AAAGGTATGT GAAGTACGGC TCCGGTCCAT CTCGCTTCCC ACTCCCCGAC 
ATGTTGAAAT ACGTTATTGA ATTTGCCAGT ACAAAACCTG CCTCTGAAAG CTGTCTGTCT 
GGAAGTGTTG AGCATATGAC GTTACCACTT CCTTCTGTGC ACTGCCCGAT TTCTGACCTC 
ACAGCCAAGG AAAGTTCAAG TCCCAAAAGC TGCTCTCAGA ATGCTGAGGG TTCCTTTTCC 
TCTCCGGAAG ATGCTCTACC CAACTCTGAG GTAATGAATG GGCCATTTAC TTCTCCTCAC 
TCTTCCCTGG AAATGCCTGC ACCCCCACCA GCTCCTCGGA CAGTCACAGA TGAGGAAATG 
AATTTCGTTA AGACCTGTCT TCAGAGGTGG CGGAGTGAAA TTGAACAGGA TATACAAGAC 
TTAAAGAATT GTATCTCGAG CACCACCCAG GCTATTGAGC AGATGTACTG TGATCCTCTT 
CTTCGTCAGG TGCCTTATCG CTTACATGCA GTTCTTGTTC ATGAAGGACA AGCCAGTGCA 
GGGCACTACT GGGCGTACAT CTATAACCAG CCCCGGCAGA TCTGGCTCAA ATACAACGAC 
ATCTCTGTCA CTGAGTCTTC CTGGGAAGAA CTTGAAAGAG ATTCTTACGG GGGCCTGAGA 
AATGTCAGCG CATACTGCTT GATGTATATC AATGACAAGT TGCCCCACTG CAGTGCAGAG 
GCAGCGCATG GTGAATCTGA TCAGACTGCA GAAGTGGAAG CCCTGTCTGT GGAACTCAGG 
CAGTACATTC AGGAAGATAA CTGGAGGTTT GAGCAAGAGG TGGAGGAATG GGAGGAAGAA 
CAGTCCTGTA AGATCCCACA GATGGAGTCC TCCCCAAACT CTTCCTCACA GGATTTTTCC 
ACATCACAAG AGTCTTCAGC AGCGTCCTCG CATGGCGTTC GATGTTTGTC TTCTGAACAT 
GCTGTGATTG CAAAGGAGCA GACTGCCCAG GCCATCGCAA ACACGGCTCA TGCCTATGAG 
AAGAGCGGGG TAGAAGCAGC CTTGAGTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
CTCCTGGCCA TAGCTAAGGC GCGCCAGACC TTTGACCGAG ACGGGTCTGA AGCAGGGCTT 
ATTAAGGCAT TTCATGAAGA GTACTCCAGG CTCTATCAGC TTGCCAAAGA GACACCCACC 
TCTCACAGTG ATCCTCGCCT TCAGCATGTT CTCGTCTACT TTTTCCAAAA TGAAGCACCC 
AAGAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAGAAACCT TAGCTATGAT 
GAGAGATCTA TCAGTATCAT GAAGGTGGCT CAGGCCAAAC TGATGGAAAT TGGTCCAGAG 
GACATGAACA TGGAAGAGTA TAAGAGGTGG CATGAAGACT ATAGTCTGTT CCGAAAGGTG 
TCTGTGTACC TGCTAACGGG CCTGGAACTC TTCCAAAAAG GAAAGTACCA GGAAGCTTTG 
TCCTACCTGG TGTACGCGTA CCAGAGCAAT GCTGGCCTGC TGGTGAAGGG GCCCCGCAGG 
GGCGTGAAGG AGTCTGTGAT TGCTTTGTAC CGCAGAAAAT GCCTTCTGGA GTTGAATGCC 
AAGGCTGCTT CTCTTTTTGA AACAAATGAC GACCATTCTG TAACAGAGGG CATTAATGTG 
ATGAATGAGC TGATCATTCC CTGCATCCAC CTGATCATTA ATAATGACAT TTCCAAGGAC 
GACCTTGATG CCATCGAGGT CATGAGGAAC CACTGGTGCT CTTACCTGGG GAAGGACATC 
GCAGAAAATC TGCAGCTGTG TTTAGGGGAG TTCCTACCCA GGCTTCTAGA TCCTTCCGCA 
GAAATCATCG TCCTAAAGGA GCCTCCAACT ATTCGACCGA ATTCTCCCTA TGATCTTTGC 
AGCCGGTTTG CAGCTGTCAT GGAGTCCATT CAAGGAGTTT CAACAGTAAC AGTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101614.1
CDS	4..3243
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	NM_001108144.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108144.1

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTTGCCT 
GAATTTCGAA GACTTGTTCT CAGTTACAGC CTGCCACAGA ACATTCTTGA AAATTGTCGA 
AGTCACACTG AAAAAAGAAA TATCATGTTT ATGCAAGAGC TTCAGTATTT ATTTGCTCTG 
TTATTGGGAT CAAATCGGAA ATTTGTAGAT CCTTCTGCTG CCCTGGATCT ACTGAAGGGG 
GCCTTCCGAT CCTCAGAGGA GCAACAGCAA GATGTGAGTG AATTCACACA CAAGCTCCTG 
GATTGGCTGG AGGATGCGTT CCAGCTAGCT GTTAATGTTA ACAGCAATCC CAGGACTAAA 
TCTGAAAACC CCATGGTGCA GCTGTTCTAC GGTACCTTCC TGACAGAAGG GGTCCGAGAA 
GGAAAACCCT TTTGTAACAA CGAGACCTTC GGCCAGTACC CTCTTCAGGT AAACGGTTAC 
CACAACTTAG ACGAGTGTTT GGAAGGGGCC ATGGTGGAAG GTGACATTGC GCTGCTTCCT 
TCTGATCGCT CAGTGAAATA CGGACAAGAG CGTTGGTTTA CAAAGCTGCC TCCAGTGTTG 
ACCTTCGAAC TCTCCAGATT TGAGTTTAAT CAGTCTCTTG GGCAGCCTGA GAAAATTCAT 
AATAAGCTGG AATTTCCTCA GGTTATTTAT ATGGATAGGT ACATGTACAA AAGCAAAGAG 
CTTATCCGAA GTAAGAGAGA GAGCATTCGA AAGTTGAAGG AGGAAATACA AGTTCTGCAG 
CAGAAATTAG AAAGGTATGT GAAGTACGGC TCCGGTCCAT CTCGCTTCCC ACTCCCCGAC 
ATGTTGAAAT ACGTTATTGA ATTTGCCAGT ACAAAACCTG CCTCTGAAAG CTGTCTGTCT 
GGAAGTGTTG AGCATATGAC GTTACCACTT CCTTCTGTGC ACTGCCCGAT TTCTGACCTC 
ACAGCCAAGG AAAGTTCAAG TCCCAAAAGC TGCTCTCAGA ATGCTGAGGG TTCCTTTTCC 
TCTCCGGAAG ATGCTCTACC CAACTCTGAG GTAATGAATG GGCCATTTAC TTCTCCTCAC 
TCTTCCCTGG AAATGCCTGC ACCCCCACCA GCTCCTCGGA CAGTCACAGA TGAGGAAATG 
AATTTCGTTA AGACCTGTCT TCAGAGGTGG CGGAGTGAAA TTGAACAGGA TATACAAGAC 
TTAAAGAATT GTATCTCGAG CACCACCCAG GCTATTGAGC AGATGTACTG TGATCCTCTT 
CTTCGTCAGG TGCCTTATCG CTTACATGCA GTTCTTGTTC ATGAAGGACA AGCCAGTGCA 
GGGCACTACT GGGCGTACAT CTATAACCAG CCCCGGCAGA TCTGGCTCAA ATACAACGAC 
ATCTCTGTCA CTGAGTCTTC CTGGGAAGAA CTTGAAAGAG ATTCTTACGG GGGCCTGAGA 
AATGTCAGCG CATACTGCTT GATGTATATC AATGACAAGT TGCCCCACTG CAGTGCAGAG 
GCAGCGCATG GTGAATCTGA TCAGACTGCA GAAGTGGAAG CCCTGTCTGT GGAACTCAGG 
CAGTACATTC AGGAAGATAA CTGGAGGTTT GAGCAAGAGG TGGAGGAATG GGAGGAAGAA 
CAGTCCTGTA AGATCCCACA GATGGAGTCC TCCCCAAACT CTTCCTCACA GGATTTTTCC 
ACATCACAAG AGTCTTCAGC AGCGTCCTCG CATGGCGTTC GATGTTTGTC TTCTGAACAT 
GCTGTGATTG CAAAGGAGCA GACTGCCCAG GCCATCGCAA ACACGGCTCA TGCCTATGAG 
AAGAGCGGGG TAGAAGCAGC CTTGAGTGAG GTGATGCTGA GCCCTGCCAT GCAAGGGGTC 
CTCCTGGCCA TAGCTAAGGC GCGCCAGACC TTTGACCGAG ACGGGTCTGA AGCAGGGCTT 
ATTAAGGCAT TTCATGAAGA GTACTCCAGG CTCTATCAGC TTGCCAAAGA GACACCCACC 
TCTCACAGTG ATCCTCGCCT TCAGCATGTT CTCGTCTACT TTTTCCAAAA TGAAGCACCC 
AAGAGGGTAG TAGAACGAAC CCTTCTGGAA CAGTTTGCAG ATAGAAACCT TAGCTATGAT 
GAGAGATCTA TCAGTATCAT GAAGGTGGCT CAGGCCAAAC TGATGGAAAT TGGTCCAGAG 
GACATGAACA TGGAAGAGTA TAAGAGGTGG CATGAAGACT ATAGTCTGTT CCGAAAGGTG 
TCTGTGTACC TGCTAACGGG CCTGGAACTC TTCCAAAAAG GAAAGTACCA GGAAGCTTTG 
TCCTACCTGG TGTACGCGTA CCAGAGCAAT GCTGGCCTGC TGGTGAAGGG GCCCCGCAGG 
GGCGTGAAGG AGTCTGTGAT TGCTTTGTAC CGCAGAAAAT GCCTTCTGGA GTTGAATGCC 
AAGGCTGCTT CTCTTTTTGA AACAAATGAC GACCATTCTG TAACAGAGGG CATTAATGTG 
ATGAATGAGC TGATCATTCC CTGCATCCAC CTGATCATTA ATAATGACAT TTCCAAGGAC 
GACCTTGATG CCATCGAGGT CATGAGGAAC CACTGGTGCT CTTACCTGGG GAAGGACATC 
GCAGAAAATC TGCAGCTGTG TTTAGGGGAG TTCCTACCCA GGCTTCTAGA TCCTTCCGCA 
GAAATCATCG TCCTAAAGGA GCCTCCAACT ATTCGACCGA ATTCTCCCTA TGATCTTTGC 
AGCCGGTTTG CAGCTGTCAT GGAGTCCATT CAAGGAGTTT CAACAGTAAC AGTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243006.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243068.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31125
Clone ID Related Accession (Same CDS sequence)	XM_006243006.3
Accession Version	XM_006243006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGCATAAGGA AGCTGAACCC 
GAGAAGCCCC AGGAAACAAA CCTTGCAGAG CAGTCAGAAC AGGCTGCAGA GGTCAAAGAC 
ACAGAGGCTG CTGCCCCACC TAACTCTGAG GTCTCTGAAG TGGAGATCCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGC TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243068.1
CDS	108..3539
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEHKEAEPEKPQETNLAEQSEQAAEVKDTEAAAPPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243006.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243006.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGCATAAGGA AGCTGAACCC 
GAGAAGCCCC AGGAAACAAA CCTTGCAGAG CAGTCAGAAC AGGCTGCAGA GGTCAAAGAC 
ACAGAGGCTG CTGCCCCACC TAACTCTGAG GTCTCTGAAG TGGAGATCCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGC TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243007.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243069.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X2, mRNA.

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CloneID	ORa31126
Clone ID Related Accession (Same CDS sequence)	XM_006243007.3
Accession Version	XM_006243007.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGC ATAAGGAAGC TGAACCCGAG 
AAGCCCCAGG AAACAAACCT TGCAGAGCAG TCAGAACAGG CTGCAGAGGT CAAAGACACA 
GAGGCTGCTG CCCCACCTAA CTCTGAGGTC TCTGAAGTGG AGATCCCCAG TGTGGGAAGG 
ATTCTGGTTA GATCTGATGC AGATGGCTAT GATGAGGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCC TCCTGGCCAT AGCTAAGGCG CGCCAGACCT TTGACCGAGA CGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT TCATGAAGAG TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACACCCACCT CTCACAGTGA TCCTCGCCTT CAGCATGTTC TCGTCTACTT TTTCCAAAAT 
GAAGCACCCA AGAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAGAAACCTT 
AGCTATGATG AGAGATCTAT CAGTATCATG AAGGTGGCTC AGGCCAAACT GATGGAAATT 
GGTCCAGAGG ACATGAACAT GGAAGAGTAT AAGAGGTGGC ATGAAGACTA TAGTCTGTTC 
CGAAAGGTGT CTGTGTACCT GCTAACGGGC CTGGAACTCT TCCAAAAAGG AAAGTACCAG 
GAAGCTTTGT CCTACCTGGT GTACGCGTAC CAGAGCAATG CTGGCCTGCT GGTGAAGGGG 
CCCCGCAGGG GCGTGAAGGA GTCTGTGATT GCTTTGTACC GCAGAAAATG CCTTCTGGAG 
TTGAATGCCA AGGCTGCTTC TCTTTTTGAA ACAAATGACG ACCATTCTGT AACAGAGGGC 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGCATCCACC TGATCATTAA TAATGACATT 
TCCAAGGACG ACCTTGATGC CATCGAGGTC ATGAGGAACC ACTGGTGCTC TTACCTGGGG 
AAGGACATCG CAGAAAATCT GCAGCTGTGT TTAGGGGAGT TCCTACCCAG GCTTCTAGAT 
CCTTCCGCAG AAATCATCGT CCTAAAGGAG CCTCCAACTA TTCGACCGAA TTCTCCCTAT 
GATCTTTGCA GCCGGTTTGC AGCTGTCATG GAGTCCATTC AAGGAGTTTC AACAGTAACA 
GTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243069.1
CDS	108..3536
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEHKEAEPEKPQETNLAEQSEQAAEVKDTEAAAPPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243007.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243007.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGC ATAAGGAAGC TGAACCCGAG 
AAGCCCCAGG AAACAAACCT TGCAGAGCAG TCAGAACAGG CTGCAGAGGT CAAAGACACA 
GAGGCTGCTG CCCCACCTAA CTCTGAGGTC TCTGAAGTGG AGATCCCCAG TGTGGGAAGG 
ATTCTGGTTA GATCTGATGC AGATGGCTAT GATGAGGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCC TCCTGGCCAT AGCTAAGGCG CGCCAGACCT TTGACCGAGA CGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT TCATGAAGAG TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACACCCACCT CTCACAGTGA TCCTCGCCTT CAGCATGTTC TCGTCTACTT TTTCCAAAAT 
GAAGCACCCA AGAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAGAAACCTT 
AGCTATGATG AGAGATCTAT CAGTATCATG AAGGTGGCTC AGGCCAAACT GATGGAAATT 
GGTCCAGAGG ACATGAACAT GGAAGAGTAT AAGAGGTGGC ATGAAGACTA TAGTCTGTTC 
CGAAAGGTGT CTGTGTACCT GCTAACGGGC CTGGAACTCT TCCAAAAAGG AAAGTACCAG 
GAAGCTTTGT CCTACCTGGT GTACGCGTAC CAGAGCAATG CTGGCCTGCT GGTGAAGGGG 
CCCCGCAGGG GCGTGAAGGA GTCTGTGATT GCTTTGTACC GCAGAAAATG CCTTCTGGAG 
TTGAATGCCA AGGCTGCTTC TCTTTTTGAA ACAAATGACG ACCATTCTGT AACAGAGGGC 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGCATCCACC TGATCATTAA TAATGACATT 
TCCAAGGACG ACCTTGATGC CATCGAGGTC ATGAGGAACC ACTGGTGCTC TTACCTGGGG 
AAGGACATCG CAGAAAATCT GCAGCTGTGT TTAGGGGAGT TCCTACCCAG GCTTCTAGAT 
CCTTCCGCAG AAATCATCGT CCTAAAGGAG CCTCCAACTA TTCGACCGAA TTCTCCCTAT 
GATCTTTGCA GCCGGTTTGC AGCTGTCATG GAGTCCATTC AAGGAGTTTC AACAGTAACA 
GTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243008.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243070.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X3, mRNA.

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CloneID	ORa31127
Clone ID Related Accession (Same CDS sequence)	XM_006243008.3
Accession Version	XM_006243008.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243070.1
CDS	108..3359
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243008.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243008.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243009.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243071.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X4, mRNA.

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CloneID	ORa31128
Clone ID Related Accession (Same CDS sequence)	XM_006243009.3
Accession Version	XM_006243009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGCATAAGGA AGCTGAACCC 
GAGAAGCCCC AGGAAACAAA CCTTGCAGAG CAGTCAGAAC AGGCTGCAGA GGTCAAAGAC 
ACAGAGGCTG CTGCCCCACC TAACTCTGAG GTCTCTGAAG TGGAGATCCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGC TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243071.1
CDS	108..3359
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEHKEAEPEKPQETNLAEQSEQAAEVKDTEAAAPPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243009.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243009.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGCATAAGGA AGCTGAACCC 
GAGAAGCCCC AGGAAACAAA CCTTGCAGAG CAGTCAGAAC AGGCTGCAGA GGTCAAAGAC 
ACAGAGGCTG CTGCCCCACC TAACTCTGAG GTCTCTGAAG TGGAGATCCC CAGTGTGGGA 
AGGATTCTGG TTAGATCTGA TGCAGATGGC TATGATGAGG AGGTGATGCT GAGCCCTGCC 
ATGCAAGGGG TCCTCCTGGC CATAGCTAAG GCGCGCCAGA CCTTTGACCG AGACGGGTCT 
GAAGCAGGGC TTATTAAGGC ATTTCATGAA GAGTACTCCA GGCTCTATCA GCTTGCCAAA 
GAGACACCCA CCTCTCACAG TGATCCTCGC CTTCAGCATG TTCTCGTCTA CTTTTTCCAA 
AATGAAGCAC CCAAGAGGGT AGTAGAACGA ACCCTTCTGG AACAGTTTGC AGATAGAAAC 
CTTAGCTATG ATGAGAGATC TATCAGTATC ATGAAGGTGG CTCAGGCCAA ACTGATGGAA 
ATTGGTCCAG AGGACATGAA CATGGAAGAG TATAAGAGGT GGCATGAAGA CTATAGTCTG 
TTCCGAAAGG TGTCTGTGTA CCTGCTAACG GGCCTGGAAC TCTTCCAAAA AGGAAAGTAC 
CAGGAAGCTT TGTCCTACCT GGTGTACGCG TACCAGAGCA ATGCTGGCCT GCTGGTGAAG 
GGGCCCCGCA GGGGCGTGAA GGAGTCTGTG ATTGCTTTGT ACCGCAGAAA ATGCCTTCTG 
GAGTTGAATG CCAAGGCTGC TTCTCTTTTT GAAACAAATG ACGACCATTC TGTAACAGAG 
GGCATTAATG TGATGAATGA GCTGATCATT CCCTGCATCC ACCTGATCAT TAATAATGAC 
ATTTCCAAGG ACGACCTTGA TGCCATCGAG GTCATGAGGA ACCACTGGTG CTCTTACCTG 
GGGAAGGACA TCGCAGAAAA TCTGCAGCTG TGTTTAGGGG AGTTCCTACC CAGGCTTCTA 
GATCCTTCCG CAGAAATCAT CGTCCTAAAG GAGCCTCCAA CTATTCGACC GAATTCTCCC 
TATGATCTTT GCAGCCGGTT TGCAGCTGTC ATGGAGTCCA TTCAAGGAGT TTCAACAGTA 
ACAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243010.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243072.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa31129
Clone ID Related Accession (Same CDS sequence)	XM_006243010.3
Accession Version	XM_006243010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCC TCCTGGCCAT AGCTAAGGCG CGCCAGACCT TTGACCGAGA CGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT TCATGAAGAG TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACACCCACCT CTCACAGTGA TCCTCGCCTT CAGCATGTTC TCGTCTACTT TTTCCAAAAT 
GAAGCACCCA AGAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAGAAACCTT 
AGCTATGATG AGAGATCTAT CAGTATCATG AAGGTGGCTC AGGCCAAACT GATGGAAATT 
GGTCCAGAGG ACATGAACAT GGAAGAGTAT AAGAGGTGGC ATGAAGACTA TAGTCTGTTC 
CGAAAGGTGT CTGTGTACCT GCTAACGGGC CTGGAACTCT TCCAAAAAGG AAAGTACCAG 
GAAGCTTTGT CCTACCTGGT GTACGCGTAC CAGAGCAATG CTGGCCTGCT GGTGAAGGGG 
CCCCGCAGGG GCGTGAAGGA GTCTGTGATT GCTTTGTACC GCAGAAAATG CCTTCTGGAG 
TTGAATGCCA AGGCTGCTTC TCTTTTTGAA ACAAATGACG ACCATTCTGT AACAGAGGGC 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGCATCCACC TGATCATTAA TAATGACATT 
TCCAAGGACG ACCTTGATGC CATCGAGGTC ATGAGGAACC ACTGGTGCTC TTACCTGGGG 
AAGGACATCG CAGAAAATCT GCAGCTGTGT TTAGGGGAGT TCCTACCCAG GCTTCTAGAT 
CCTTCCGCAG AAATCATCGT CCTAAAGGAG CCTCCAACTA TTCGACCGAA TTCTCCCTAT 
GATCTTTGCA GCCGGTTTGC AGCTGTCATG GAGTCCATTC AAGGAGTTTC AACAGTAACA 
GTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243072.1
CDS	108..3356
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEVMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243010.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243010.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGG TGATGCTGAG CCCTGCCATG 
CAAGGGGTCC TCCTGGCCAT AGCTAAGGCG CGCCAGACCT TTGACCGAGA CGGGTCTGAA 
GCAGGGCTTA TTAAGGCATT TCATGAAGAG TACTCCAGGC TCTATCAGCT TGCCAAAGAG 
ACACCCACCT CTCACAGTGA TCCTCGCCTT CAGCATGTTC TCGTCTACTT TTTCCAAAAT 
GAAGCACCCA AGAGGGTAGT AGAACGAACC CTTCTGGAAC AGTTTGCAGA TAGAAACCTT 
AGCTATGATG AGAGATCTAT CAGTATCATG AAGGTGGCTC AGGCCAAACT GATGGAAATT 
GGTCCAGAGG ACATGAACAT GGAAGAGTAT AAGAGGTGGC ATGAAGACTA TAGTCTGTTC 
CGAAAGGTGT CTGTGTACCT GCTAACGGGC CTGGAACTCT TCCAAAAAGG AAAGTACCAG 
GAAGCTTTGT CCTACCTGGT GTACGCGTAC CAGAGCAATG CTGGCCTGCT GGTGAAGGGG 
CCCCGCAGGG GCGTGAAGGA GTCTGTGATT GCTTTGTACC GCAGAAAATG CCTTCTGGAG 
TTGAATGCCA AGGCTGCTTC TCTTTTTGAA ACAAATGACG ACCATTCTGT AACAGAGGGC 
ATTAATGTGA TGAATGAGCT GATCATTCCC TGCATCCACC TGATCATTAA TAATGACATT 
TCCAAGGACG ACCTTGATGC CATCGAGGTC ATGAGGAACC ACTGGTGCTC TTACCTGGGG 
AAGGACATCG CAGAAAATCT GCAGCTGTGT TTAGGGGAGT TCCTACCCAG GCTTCTAGAT 
CCTTCCGCAG AAATCATCGT CCTAAAGGAG CCTCCAACTA TTCGACCGAA TTCTCCCTAT 
GATCTTTGCA GCCGGTTTGC AGCTGTCATG GAGTCCATTC AAGGAGTTTC AACAGTAACA 
GTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243011.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243073.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa31130
Clone ID Related Accession (Same CDS sequence)	XM_006243011.3
Accession Version	XM_006243011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGGCATTTCA TGAAGAGTAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACA CCCACCTCTC ACAGTGATCC TCGCCTTCAG 
CATGTTCTCG TCTACTTTTT CCAAAATGAA GCACCCAAGA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAG AAACCTTAGC TATGATGAGA GATCTATCAG TATCATGAAG 
GTGGCTCAGG CCAAACTGAT GGAAATTGGT CCAGAGGACA TGAACATGGA AGAGTATAAG 
AGGTGGCATG AAGACTATAG TCTGTTCCGA AAGGTGTCTG TGTACCTGCT AACGGGCCTG 
GAACTCTTCC AAAAAGGAAA GTACCAGGAA GCTTTGTCCT ACCTGGTGTA CGCGTACCAG 
AGCAATGCTG GCCTGCTGGT GAAGGGGCCC CGCAGGGGCG TGAAGGAGTC TGTGATTGCT 
TTGTACCGCA GAAAATGCCT TCTGGAGTTG AATGCCAAGG CTGCTTCTCT TTTTGAAACA 
AATGACGACC ATTCTGTAAC AGAGGGCATT AATGTGATGA ATGAGCTGAT CATTCCCTGC 
ATCCACCTGA TCATTAATAA TGACATTTCC AAGGACGACC TTGATGCCAT CGAGGTCATG 
AGGAACCACT GGTGCTCTTA CCTGGGGAAG GACATCGCAG AAAATCTGCA GCTGTGTTTA 
GGGGAGTTCC TACCCAGGCT TCTAGATCCT TCCGCAGAAA TCATCGTCCT AAAGGAGCCT 
CCAACTATTC GACCGAATTC TCCCTATGAT CTTTGCAGCC GGTTTGCAGC TGTCATGGAG 
TCCATTCAAG GAGTTTCAAC AGTAACAGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243073.1
CDS	108..3263
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243011.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243011.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAGCAAT 
CCCAGGACTA AATCTGAAAA CCCCATGGTG CAGCTGTTCT ACGGTACCTT CCTGACAGAA 
GGGGTCCGAG AAGGAAAACC CTTTTGTAAC AACGAGACCT TCGGCCAGTA CCCTCTTCAG 
GTAAACGGTT ACCACAACTT AGACGAGTGT TTGGAAGGGG CCATGGTGGA AGGTGACATT 
GCGCTGCTTC CTTCTGATCG CTCAGTGAAA TACGGACAAG AGCGTTGGTT TACAAAGCTG 
CCTCCAGTGT TGACCTTCGA ACTCTCCAGA TTTGAGTTTA ATCAGTCTCT TGGGCAGCCT 
GAGAAAATTC ATAATAAGCT GGAATTTCCT CAGGTTATTT ATATGGATAG GTACATGTAC 
AAAAGCAAAG AGCTTATCCG AAGTAAGAGA GAGAGCATTC GAAAGTTGAA GGAGGAAATA 
CAAGTTCTGC AGCAGAAATT AGAAAGGTAT GTGAAGTACG GCTCCGGTCC ATCTCGCTTC 
CCACTCCCCG ACATGTTGAA ATACGTTATT GAATTTGCCA GTACAAAACC TGCCTCTGAA 
AGCTGTCTGT CTGGAAGTGT TGAGCATATG ACGTTACCAC TTCCTTCTGT GCACTGCCCG 
ATTTCTGACC TCACAGCCAA GGAAAGTTCA AGTCCCAAAA GCTGCTCTCA GAATGCTGAG 
GGTTCCTTTT CCTCTCCGGA AGATGCTCTA CCCAACTCTG AGGTAATGAA TGGGCCATTT 
ACTTCTCCTC ACTCTTCCCT GGAAATGCCT GCACCCCCAC CAGCTCCTCG GACAGTCACA 
GATGAGGAAA TGAATTTCGT TAAGACCTGT CTTCAGAGGT GGCGGAGTGA AATTGAACAG 
GATATACAAG ACTTAAAGAA TTGTATCTCG AGCACCACCC AGGCTATTGA GCAGATGTAC 
TGTGATCCTC TTCTTCGTCA GGTGCCTTAT CGCTTACATG CAGTTCTTGT TCATGAAGGA 
CAAGCCAGTG CAGGGCACTA CTGGGCGTAC ATCTATAACC AGCCCCGGCA GATCTGGCTC 
AAATACAACG ACATCTCTGT CACTGAGTCT TCCTGGGAAG AACTTGAAAG AGATTCTTAC 
GGGGGCCTGA GAAATGTCAG CGCATACTGC TTGATGTATA TCAATGACAA GTTGCCCCAC 
TGCAGTGCAG AGGCAGCGCA TGGTGAATCT GATCAGACTG CAGAAGTGGA AGCCCTGTCT 
GTGGAACTCA GGCAGTACAT TCAGGAAGAT AACTGGAGGT TTGAGCAAGA GGTGGAGGAA 
TGGGAGGAAG AACAGTCCTG TAAGATCCCA CAGATGGAGT CCTCCCCAAA CTCTTCCTCA 
CAGGATTTTT CCACATCACA AGAGTCTTCA GCAGCGTCCT CGCATGGCGT TCGATGTTTG 
TCTTCTGAAC ATGCTGTGAT TGCAAAGGAG CAGACTGCCC AGGCCATCGC AAACACGGCT 
CATGCCTATG AGAAGAGCGG GGTAGAAGCA GCCTTGAGTG AGGCATTTCA TGAAGAGTAC 
TCCAGGCTCT ATCAGCTTGC CAAAGAGACA CCCACCTCTC ACAGTGATCC TCGCCTTCAG 
CATGTTCTCG TCTACTTTTT CCAAAATGAA GCACCCAAGA GGGTAGTAGA ACGAACCCTT 
CTGGAACAGT TTGCAGATAG AAACCTTAGC TATGATGAGA GATCTATCAG TATCATGAAG 
GTGGCTCAGG CCAAACTGAT GGAAATTGGT CCAGAGGACA TGAACATGGA AGAGTATAAG 
AGGTGGCATG AAGACTATAG TCTGTTCCGA AAGGTGTCTG TGTACCTGCT AACGGGCCTG 
GAACTCTTCC AAAAAGGAAA GTACCAGGAA GCTTTGTCCT ACCTGGTGTA CGCGTACCAG 
AGCAATGCTG GCCTGCTGGT GAAGGGGCCC CGCAGGGGCG TGAAGGAGTC TGTGATTGCT 
TTGTACCGCA GAAAATGCCT TCTGGAGTTG AATGCCAAGG CTGCTTCTCT TTTTGAAACA 
AATGACGACC ATTCTGTAAC AGAGGGCATT AATGTGATGA ATGAGCTGAT CATTCCCTGC 
ATCCACCTGA TCATTAATAA TGACATTTCC AAGGACGACC TTGATGCCAT CGAGGTCATG 
AGGAACCACT GGTGCTCTTA CCTGGGGAAG GACATCGCAG AAAATCTGCA GCTGTGTTTA 
GGGGAGTTCC TACCCAGGCT TCTAGATCCT TCCGCAGAAA TCATCGTCCT AAAGGAGCCT 
CCAACTATTC GACCGAATTC TCCCTATGAT CTTTGCAGCC GGTTTGCAGC TGTCATGGAG 
TCCATTCAAG GAGTTTCAAC AGTAACAGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp28 ( XM_006243012.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243074.1 Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X7, mRNA.

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CloneID	ORa31131
Clone ID Related Accession (Same CDS sequence)	XM_006243012.3
Accession Version	XM_006243012.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGG CATTTCATGA AGAGTACTCC 
AGGCTCTATC AGCTTGCCAA AGAGACACCC ACCTCTCACA GTGATCCTCG CCTTCAGCAT 
GTTCTCGTCT ACTTTTTCCA AAATGAAGCA CCCAAGAGGG TAGTAGAACG AACCCTTCTG 
GAACAGTTTG CAGATAGAAA CCTTAGCTAT GATGAGAGAT CTATCAGTAT CATGAAGGTG 
GCTCAGGCCA AACTGATGGA AATTGGTCCA GAGGACATGA ACATGGAAGA GTATAAGAGG 
TGGCATGAAG ACTATAGTCT GTTCCGAAAG GTGTCTGTGT ACCTGCTAAC GGGCCTGGAA 
CTCTTCCAAA AAGGAAAGTA CCAGGAAGCT TTGTCCTACC TGGTGTACGC GTACCAGAGC 
AATGCTGGCC TGCTGGTGAA GGGGCCCCGC AGGGGCGTGA AGGAGTCTGT GATTGCTTTG 
TACCGCAGAA AATGCCTTCT GGAGTTGAAT GCCAAGGCTG CTTCTCTTTT TGAAACAAAT 
GACGACCATT CTGTAACAGA GGGCATTAAT GTGATGAATG AGCTGATCAT TCCCTGCATC 
CACCTGATCA TTAATAATGA CATTTCCAAG GACGACCTTG ATGCCATCGA GGTCATGAGG 
AACCACTGGT GCTCTTACCT GGGGAAGGAC ATCGCAGAAA ATCTGCAGCT GTGTTTAGGG 
GAGTTCCTAC CCAGGCTTCT AGATCCTTCC GCAGAAATCA TCGTCCTAAA GGAGCCTCCA 
ACTATTCGAC CGAATTCTCC CTATGATCTT TGCAGCCGGT TTGCAGCTGT CATGGAGTCC 
ATTCAAGGAG TTTCAACAGT AACAGTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243074.1
CDS	108..3260
Translation	MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDTAATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRKRCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNNPRTKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEELERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

Target ORF information:

RefSeq Version	XM_006243012.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 28 (Usp28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243012.3

ORF Insert Sequence:

ATGACTGCGG AGCTGCAGCA GGACGACGCG GCGGGGGCGG CGGACGGCCA TGGCTCGAGC 
TGCCAGATGC TGTTAAATCA GCTGCGAGAA ATCACGGGCA TCCAGGATCC TTCCTTTCTT 
CATGAAGCAT TAAAGGCCAG TAATGGTGAC ATCACCCAGG CTGTCAGCCT TCTCACAGAC 
CAAAGAGTTA AGGAGCCCAG TCACGACACA GCTGCTACAG AACCATCTGA AGTAGAAGAG 
AGTGCTACCA GCAAAGATCT CTTAGCAAAA GTGATAGACC TTACACATGA CAACAAAGAT 
GACCTTCAGG CAGCCATTGC CTTGAGTCTC CTGGAGTCTC CTAACATTCA GACTGACAGC 
AGAGATCTTA ATAGAATACA TGAAGCTAAC TCTGCAGAGA CTAAACGATC AAAGAGAAAA 
CGCTGTGAAG TTTGGGGAGA AAACCACAAT CCCAATAACT GGAGGAGAGT GGATGGTTGG 
CCAGTTGGGC TGAAAAATGT TGGCAACACA TGTTGGTTCA GTGCTGTTAT TCAGTCTCTC 
TTTCAGTTGC CTGAATTTCG AAGACTTGTT CTCAGTTACA GCCTGCCACA GAACATTCTT 
GAAAATTGTC GAAGTCACAC TGAAAAAAGA AATATCATGT TTATGCAAGA GCTTCAGTAT 
TTATTTGCTC TGTTATTGGG ATCAAATCGG AAATTTGTAG ATCCTTCTGC TGCCCTGGAT 
CTACTGAAGG GGGCCTTCCG ATCCTCAGAG GAGCAACAGC AAGATGTGAG TGAATTCACA 
CACAAGCTCC TGGATTGGCT GGAGGATGCG TTCCAGCTAG CTGTTAATGT TAACAATCCC 
AGGACTAAAT CTGAAAACCC CATGGTGCAG CTGTTCTACG GTACCTTCCT GACAGAAGGG 
GTCCGAGAAG GAAAACCCTT TTGTAACAAC GAGACCTTCG GCCAGTACCC TCTTCAGGTA 
AACGGTTACC ACAACTTAGA CGAGTGTTTG GAAGGGGCCA TGGTGGAAGG TGACATTGCG 
CTGCTTCCTT CTGATCGCTC AGTGAAATAC GGACAAGAGC GTTGGTTTAC AAAGCTGCCT 
CCAGTGTTGA CCTTCGAACT CTCCAGATTT GAGTTTAATC AGTCTCTTGG GCAGCCTGAG 
AAAATTCATA ATAAGCTGGA ATTTCCTCAG GTTATTTATA TGGATAGGTA CATGTACAAA 
AGCAAAGAGC TTATCCGAAG TAAGAGAGAG AGCATTCGAA AGTTGAAGGA GGAAATACAA 
GTTCTGCAGC AGAAATTAGA AAGGTATGTG AAGTACGGCT CCGGTCCATC TCGCTTCCCA 
CTCCCCGACA TGTTGAAATA CGTTATTGAA TTTGCCAGTA CAAAACCTGC CTCTGAAAGC 
TGTCTGTCTG GAAGTGTTGA GCATATGACG TTACCACTTC CTTCTGTGCA CTGCCCGATT 
TCTGACCTCA CAGCCAAGGA AAGTTCAAGT CCCAAAAGCT GCTCTCAGAA TGCTGAGGGT 
TCCTTTTCCT CTCCGGAAGA TGCTCTACCC AACTCTGAGG TAATGAATGG GCCATTTACT 
TCTCCTCACT CTTCCCTGGA AATGCCTGCA CCCCCACCAG CTCCTCGGAC AGTCACAGAT 
GAGGAAATGA ATTTCGTTAA GACCTGTCTT CAGAGGTGGC GGAGTGAAAT TGAACAGGAT 
ATACAAGACT TAAAGAATTG TATCTCGAGC ACCACCCAGG CTATTGAGCA GATGTACTGT 
GATCCTCTTC TTCGTCAGGT GCCTTATCGC TTACATGCAG TTCTTGTTCA TGAAGGACAA 
GCCAGTGCAG GGCACTACTG GGCGTACATC TATAACCAGC CCCGGCAGAT CTGGCTCAAA 
TACAACGACA TCTCTGTCAC TGAGTCTTCC TGGGAAGAAC TTGAAAGAGA TTCTTACGGG 
GGCCTGAGAA ATGTCAGCGC ATACTGCTTG ATGTATATCA ATGACAAGTT GCCCCACTGC 
AGTGCAGAGG CAGCGCATGG TGAATCTGAT CAGACTGCAG AAGTGGAAGC CCTGTCTGTG 
GAACTCAGGC AGTACATTCA GGAAGATAAC TGGAGGTTTG AGCAAGAGGT GGAGGAATGG 
GAGGAAGAAC AGTCCTGTAA GATCCCACAG ATGGAGTCCT CCCCAAACTC TTCCTCACAG 
GATTTTTCCA CATCACAAGA GTCTTCAGCA GCGTCCTCGC ATGGCGTTCG ATGTTTGTCT 
TCTGAACATG CTGTGATTGC AAAGGAGCAG ACTGCCCAGG CCATCGCAAA CACGGCTCAT 
GCCTATGAGA AGAGCGGGGT AGAAGCAGCC TTGAGTGAGG CATTTCATGA AGAGTACTCC 
AGGCTCTATC AGCTTGCCAA AGAGACACCC ACCTCTCACA GTGATCCTCG CCTTCAGCAT 
GTTCTCGTCT ACTTTTTCCA AAATGAAGCA CCCAAGAGGG TAGTAGAACG AACCCTTCTG 
GAACAGTTTG CAGATAGAAA CCTTAGCTAT GATGAGAGAT CTATCAGTAT CATGAAGGTG 
GCTCAGGCCA AACTGATGGA AATTGGTCCA GAGGACATGA ACATGGAAGA GTATAAGAGG 
TGGCATGAAG ACTATAGTCT GTTCCGAAAG GTGTCTGTGT ACCTGCTAAC GGGCCTGGAA 
CTCTTCCAAA AAGGAAAGTA CCAGGAAGCT TTGTCCTACC TGGTGTACGC GTACCAGAGC 
AATGCTGGCC TGCTGGTGAA GGGGCCCCGC AGGGGCGTGA AGGAGTCTGT GATTGCTTTG 
TACCGCAGAA AATGCCTTCT GGAGTTGAAT GCCAAGGCTG CTTCTCTTTT TGAAACAAAT 
GACGACCATT CTGTAACAGA GGGCATTAAT GTGATGAATG AGCTGATCAT TCCCTGCATC 
CACCTGATCA TTAATAATGA CATTTCCAAG GACGACCTTG ATGCCATCGA GGTCATGAGG 
AACCACTGGT GCTCTTACCT GGGGAAGGAC ATCGCAGAAA ATCTGCAGCT GTGTTTAGGG 
GAGTTCCTAC CCAGGCTTCT AGATCCTTCC GCAGAAATCA TCGTCCTAAA GGAGCCTCCA 
ACTATTCGAC CGAATTCTCC CTATGATCTT TGCAGCCGGT TTGCAGCTGT CATGGAGTCC 
ATTCAAGGAG TTTCAACAGT AACAGTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
eLife3e02313(2014 Mar)
Serra RW,Fang M,Park SM,Hutchinson L,Green MR

The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint.
Cell134(2)256-67(2008 Jul)
Bassermann F,Frescas D,Guardavaccaro D,Busino L,Peschiaroli A,Pagano M

Fbw7 and Usp28 regulate myc protein stability in response to DNA damage.
Cell cycle (Georgetown, Tex.)6(19)2327-31(2007 Oct)
Popov N,Herold S,Llamazares M,Sch?lein C,Eilers M

The ubiquitin-specific protease USP28 is required for MYC stability.
Nature cell biology9(7)765-74(2007 Jul)
Popov N,Wanzel M,Madiredjo M,Zhang D,Beijersbergen R,Bernards R,Moll R,Elledge SJ,Eilers M

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Usp28 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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