ORF cDNA Clones and Custom Clones » Species Summary » Rattus norvegicus » Pikfyve cDNA ORF clone

Pikfyve cDNA ORF clone, Rattus norvegicus(Norway rat)

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Summary

Gene Symbol	Pikfyve
Entrez Gene ID	316457
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
Synonyms	Pip5k3
General protein information	Preferred Names phosphoinositide kinase, FYVE-type zinc finger containing Names 1-phosphatidylinositol-3-phosphate 5-kinase 1-phosphatidylinositol 3-phosphate 5-kinase phosphatidylinositol-3-phosphate 5-kinase type III phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III phosphoinositide kinase, FYVE finger containing
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q32

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006226860.3	XP_006226922.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006245076.3	XP_006245138.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006226857.3	XP_006226919.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006226861.2	XP_006226923.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_006245073.2	XP_006245135.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_006245077.3	XP_006245139.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006245078.2	XP_006245140.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_008758042.1	XP_008756264.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_006226856.2	XP_006226918.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_006226859.3	XP_006226921.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006226862.2	XP_006226924.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_006245071.2	XP_006245133.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_006245074.3	XP_006245136.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_006245072.2	XP_006245134.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_006245079.2	XP_006245141.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_008767170.1	XP_008765392.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_006226854.2	XP_006226916.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_006226855.2	XP_006226917.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2

Homologies

Mus musculus (house mouse)	Pikfyve	NP_035216.2
Canis lupus familiaris (dog)	PIKFYVE	XP_005640650.1
Rattus norvegicus (Norway rat)	Pikfyve	XP_006245139.1
Gallus gallus (chicken)	PIKFYVE	XP_004942735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005216	XP_314118.4
Drosophila melanogaster (fruit fly)	fab1	NP_995880.1
Homo sapiens (human)	PIKFYVE	NP_055855.2
Bos taurus (cattle)	PIKFYVE	NP_001192396.1
Pan troglodytes (chimpanzee)	PIKFYVE	XP_516057.2
	PIKFYVE	XP_001108540.1
Danio rerio (zebrafish)	pikfyve	NP_001120777.1

The following Pikfyve gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pikfyve cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa47544	XM_008758042.1 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa47544	XM_008767170.1 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa18421	XM_006226854.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18421	XM_006245071.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18422	XM_006226855.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18422	XM_006245072.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18423	XM_006226856.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18423	XM_006245073.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006245074.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006245076.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006226859.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006226860.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006245077.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18424	XM_006226857.3 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18425	XM_006226861.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18425	XM_006245078.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18426	XM_006226862.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa18426	XM_006245079.2 Latest version!	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pikfyve ( XM_008758042.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756264.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ORa47544
Clone ID Related Accession (Same CDS sequence)	XM_008758042.1 , XM_008767170.1
Accession Version	XM_008758042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756264.1
CDS	270..2012
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_008758042.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758042.1

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_008767170.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765392.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ORa47544
Clone ID Related Accession (Same CDS sequence)	XM_008758042.1 , XM_008767170.1
Accession Version	XM_008767170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765392.1
CDS	270..2012
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_008767170.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767170.1

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245071.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245133.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

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CloneID	ORa18421
Clone ID Related Accession (Same CDS sequence)	XM_006245071.2 , XM_006226854.2
Accession Version	XM_006245071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006245071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245133.1
CDS	270..6659
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245071.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245071.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226854.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226916.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

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CloneID	ORa18421
Clone ID Related Accession (Same CDS sequence)	XM_006245071.2 , XM_006226854.2
Accession Version	XM_006226854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006226854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226916.1
CDS	270..6659
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226854.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226854.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245072.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245134.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

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CloneID	ORa18422
Clone ID Related Accession (Same CDS sequence)	XM_006245072.2 , XM_006226855.2
Accession Version	XM_006245072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006245072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGGG TAGAAGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG GGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCTTA CTCCAGCAGC TGCTTCAAAA TGAGTCATTG 
TCATCATCTT GGAGGGACAT CATTGTGTCA CTAGTTTGTC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCCGTG GTTGTCAATG GCTTTGTTTG TACCAAAAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACTT GCATTGACCC CATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTCGTTG AGAAAACAGT GTCTCGGATT GCTCAAGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGA 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACCTGGGCAC TTGCCACAAG 
TTTTATATGC AGCTCTTTCA GTTGCCTAAC GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTGCAC AGCACCTCGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCCTTTC TCATGGATGA GTTTGCTATG CCTCCAACGT TAACACAAAG CCCTTCGTTC 
CATTTTCTGG CTGAGGGAAG AGGGGAAGAG GGAGCTTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCCC AGGATCCTGA GTGCCCCTTT GATGCCCTGT CTTCTGATGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCC TGAGAAGGGT GAGCTGGACA ATAAAAGTGT CCCACAGGCT 
GTTGCCTCCT TGAAGCATCA AGATTACACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CACTGGTACC CGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAGATG AACAGCCAGA GACATCACAG CAAACGGTTG AGCAACAGGA CCCCAAAAGC 
CAGATGAGAG CTTTTAGAGA CCCTCTACAG GATGACACTG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TACTCCTTGA CATTTAAACA GGAATTAAAA 
GACGTCATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GGTGCTCAAC TCGAGATTAC TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAATAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGAGCATGAA TGGCAATGTT CAGACCAAGT CCATACAAGT CTTACCTTCA 
CATGAGCTAG TGAGCACCAG GATCGCTGAA CATCTGGGGG ACAGCCAGAG CTTGGGTAGA 
ATGCTAGCTG ACTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATACATC ATGTACTTCA GGGAGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTCTTATGGC CAACAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGCGTG CTCTTCAGCA GTTCTTCTGC CCAGTCCAGC 
AATGCGCCCA GTGCTTGTGT CAGCCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CGATGTCATT TGCAAAATAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACTCGCAGAG CCAATGCTGA GCCCTGCGGT CACTCCATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ATCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTG 
TGTGTTCCAC TGCCAAAAAT ATTCATTAAG CGCCAAGCCC CGCTGAAGGT GTCTCTTCTT 
CAGGACCTCA AGGACTTCTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAAAGA 
CTTGCGTCCT TGAAAACAGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAGGATATC 
TTTGCACAAA AGGAGATGGA GGAGGGTGAG TTTAAGAGCT GGACTGAGAA GATGCAGGCG 
CGGCTCATGT CCTCGTGTGT GGACACCCCG CAGCAACTGC AGTCCGTTCT CGAGTCACTC 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGCG CTCCAAGCGT GGAACAGCAG GTTACAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGGAAGAGG CCTTCGGTTC CTCCCAGTCC TGGAAGACTG 
AGACAAGGAG AAGAGAGCAA GATAAGTGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ACGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG TCAGAGCTCC 
ACGAGCTCCA CCCATCTCCA GCTGCCCACT CCTCCTGAGG CCCTGGCTGA GCAGTTAGTG 
GGAGGGCCTT CTGACCTGGA CACAGCCAGT GGCTCTGAAG ATGTATTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA TAATCCCATT CCATTTCCTT TCGATCCAGA TAAACATTAT 
TTAATGTATG AACACGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGTGGA ACAATGCTGA AGAAGGGCTT CCAACCAATA GTGCTTTAGA TAACAGACCA 
AAAAGTAGCA GTCCTATTAG ATTACCTGAA GTCAGTGGAG GACAGACAAA CCGTACAGTA 
GAAGGAGAAC CTCAACCAAC CAAAAAAGCT TCAGGAATGC TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCTCAG AAGAGAGAGA CCTTACGTGG GGCCGACAGT 
GCATACTACC AGGTTGGGCA GACTGGCAAG GAGGGGATGG AGAGTCAAGG CCTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGACACACAG AAGAAACAAC TCACAAATCC CCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGTCGAC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GGGAAGTGAT TCTGGGCAGC AGTGAGGAAG ATTTCATTCG TTCCCTTTCT 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACAGAA 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCGCACT ACTTCAATTA TATCACCAAT GCTGTTCAAC AGAAGAGGCC CACTGCTTTG 
GCTAAAATTC TTGGTGTGTA CAGAATTGGT TACAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTGAAAACTG ACACTGGGAA AGAGAGCTGT 
GACGTGGTTC TGCTGGATGA GAACCTCCTG AAGATGGTTC GAGACAACCC TCTGTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCATA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTTTGCTG GTTGGTCGAG ATGACACTAG CAATGAGCTG 
GTTGTTGGGA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT CGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACCGTGGT CTCTCCAGAG 
TTATACAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTCAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245134.1
CDS	270..6656
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245072.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245072.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGGG TAGAAGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG GGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCTTA CTCCAGCAGC TGCTTCAAAA TGAGTCATTG 
TCATCATCTT GGAGGGACAT CATTGTGTCA CTAGTTTGTC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCCGTG GTTGTCAATG GCTTTGTTTG TACCAAAAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACTT GCATTGACCC CATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTCGTTG AGAAAACAGT GTCTCGGATT GCTCAAGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGA 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACCTGGGCAC TTGCCACAAG 
TTTTATATGC AGCTCTTTCA GTTGCCTAAC GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTGCAC AGCACCTCGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCCTTTC TCATGGATGA GTTTGCTATG CCTCCAACGT TAACACAAAG CCCTTCGTTC 
CATTTTCTGG CTGAGGGAAG AGGGGAAGAG GGAGCTTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCCC AGGATCCTGA GTGCCCCTTT GATGCCCTGT CTTCTGATGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCC TGAGAAGGGT GAGCTGGACA ATAAAAGTGT CCCACAGGCT 
GTTGCCTCCT TGAAGCATCA AGATTACACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CACTGGTACC CGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAGATG AACAGCCAGA GACATCACAG CAAACGGTTG AGCAACAGGA CCCCAAAAGC 
CAGATGAGAG CTTTTAGAGA CCCTCTACAG GATGACACTG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TACTCCTTGA CATTTAAACA GGAATTAAAA 
GACGTCATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GGTGCTCAAC TCGAGATTAC TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAATAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGAGCATGAA TGGCAATGTT CAGACCAAGT CCATACAAGT CTTACCTTCA 
CATGAGCTAG TGAGCACCAG GATCGCTGAA CATCTGGGGG ACAGCCAGAG CTTGGGTAGA 
ATGCTAGCTG ACTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATACATC ATGTACTTCA GGGAGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTCTTATGGC CAACAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGCGTG CTCTTCAGCA GTTCTTCTGC CCAGTCCAGC 
AATGCGCCCA GTGCTTGTGT CAGCCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CGATGTCATT TGCAAAATAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACTCGCAGAG CCAATGCTGA GCCCTGCGGT CACTCCATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ATCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTG 
TGTGTTCCAC TGCCAAAAAT ATTCATTAAG CGCCAAGCCC CGCTGAAGGT GTCTCTTCTT 
CAGGACCTCA AGGACTTCTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAAAGA 
CTTGCGTCCT TGAAAACAGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAGGATATC 
TTTGCACAAA AGGAGATGGA GGAGGGTGAG TTTAAGAGCT GGACTGAGAA GATGCAGGCG 
CGGCTCATGT CCTCGTGTGT GGACACCCCG CAGCAACTGC AGTCCGTTCT CGAGTCACTC 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGCG CTCCAAGCGT GGAACAGCAG GTTACAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGGAAGAGG CCTTCGGTTC CTCCCAGTCC TGGAAGACTG 
AGACAAGGAG AAGAGAGCAA GATAAGTGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ACGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG TCAGAGCTCC 
ACGAGCTCCA CCCATCTCCA GCTGCCCACT CCTCCTGAGG CCCTGGCTGA GCAGTTAGTG 
GGAGGGCCTT CTGACCTGGA CACAGCCAGT GGCTCTGAAG ATGTATTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA TAATCCCATT CCATTTCCTT TCGATCCAGA TAAACATTAT 
TTAATGTATG AACACGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGTGGA ACAATGCTGA AGAAGGGCTT CCAACCAATA GTGCTTTAGA TAACAGACCA 
AAAAGTAGCA GTCCTATTAG ATTACCTGAA GTCAGTGGAG GACAGACAAA CCGTACAGTA 
GAAGGAGAAC CTCAACCAAC CAAAAAAGCT TCAGGAATGC TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCTCAG AAGAGAGAGA CCTTACGTGG GGCCGACAGT 
GCATACTACC AGGTTGGGCA GACTGGCAAG GAGGGGATGG AGAGTCAAGG CCTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGACACACAG AAGAAACAAC TCACAAATCC CCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGTCGAC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GGGAAGTGAT TCTGGGCAGC AGTGAGGAAG ATTTCATTCG TTCCCTTTCT 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACAGAA 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCGCACT ACTTCAATTA TATCACCAAT GCTGTTCAAC AGAAGAGGCC CACTGCTTTG 
GCTAAAATTC TTGGTGTGTA CAGAATTGGT TACAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTGAAAACTG ACACTGGGAA AGAGAGCTGT 
GACGTGGTTC TGCTGGATGA GAACCTCCTG AAGATGGTTC GAGACAACCC TCTGTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCATA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTTTGCTG GTTGGTCGAG ATGACACTAG CAATGAGCTG 
GTTGTTGGGA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT CGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACCGTGGT CTCTCCAGAG 
TTATACAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTCAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226855.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226917.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

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CloneID	ORa18422
Clone ID Related Accession (Same CDS sequence)	XM_006245072.2 , XM_006226855.2
Accession Version	XM_006226855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006226855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGGG TAGAAGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG GGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCTTA CTCCAGCAGC TGCTTCAAAA TGAGTCATTG 
TCATCATCTT GGAGGGACAT CATTGTGTCA CTAGTTTGTC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCCGTG GTTGTCAATG GCTTTGTTTG TACCAAAAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACTT GCATTGACCC CATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTCGTTG AGAAAACAGT GTCTCGGATT GCTCAAGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGA 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACCTGGGCAC TTGCCACAAG 
TTTTATATGC AGCTCTTTCA GTTGCCTAAC GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTGCAC AGCACCTCGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCCTTTC TCATGGATGA GTTTGCTATG CCTCCAACGT TAACACAAAG CCCTTCGTTC 
CATTTTCTGG CTGAGGGAAG AGGGGAAGAG GGAGCTTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCCC AGGATCCTGA GTGCCCCTTT GATGCCCTGT CTTCTGATGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCC TGAGAAGGGT GAGCTGGACA ATAAAAGTGT CCCACAGGCT 
GTTGCCTCCT TGAAGCATCA AGATTACACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CACTGGTACC CGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAGATG AACAGCCAGA GACATCACAG CAAACGGTTG AGCAACAGGA CCCCAAAAGC 
CAGATGAGAG CTTTTAGAGA CCCTCTACAG GATGACACTG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TACTCCTTGA CATTTAAACA GGAATTAAAA 
GACGTCATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GGTGCTCAAC TCGAGATTAC TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAATAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGAGCATGAA TGGCAATGTT CAGACCAAGT CCATACAAGT CTTACCTTCA 
CATGAGCTAG TGAGCACCAG GATCGCTGAA CATCTGGGGG ACAGCCAGAG CTTGGGTAGA 
ATGCTAGCTG ACTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATACATC ATGTACTTCA GGGAGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTCTTATGGC CAACAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGCGTG CTCTTCAGCA GTTCTTCTGC CCAGTCCAGC 
AATGCGCCCA GTGCTTGTGT CAGCCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CGATGTCATT TGCAAAATAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACTCGCAGAG CCAATGCTGA GCCCTGCGGT CACTCCATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ATCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTG 
TGTGTTCCAC TGCCAAAAAT ATTCATTAAG CGCCAAGCCC CGCTGAAGGT GTCTCTTCTT 
CAGGACCTCA AGGACTTCTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAAAGA 
CTTGCGTCCT TGAAAACAGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAGGATATC 
TTTGCACAAA AGGAGATGGA GGAGGGTGAG TTTAAGAGCT GGACTGAGAA GATGCAGGCG 
CGGCTCATGT CCTCGTGTGT GGACACCCCG CAGCAACTGC AGTCCGTTCT CGAGTCACTC 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGCG CTCCAAGCGT GGAACAGCAG GTTACAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGGAAGAGG CCTTCGGTTC CTCCCAGTCC TGGAAGACTG 
AGACAAGGAG AAGAGAGCAA GATAAGTGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ACGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG TCAGAGCTCC 
ACGAGCTCCA CCCATCTCCA GCTGCCCACT CCTCCTGAGG CCCTGGCTGA GCAGTTAGTG 
GGAGGGCCTT CTGACCTGGA CACAGCCAGT GGCTCTGAAG ATGTATTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA TAATCCCATT CCATTTCCTT TCGATCCAGA TAAACATTAT 
TTAATGTATG AACACGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGTGGA ACAATGCTGA AGAAGGGCTT CCAACCAATA GTGCTTTAGA TAACAGACCA 
AAAAGTAGCA GTCCTATTAG ATTACCTGAA GTCAGTGGAG GACAGACAAA CCGTACAGTA 
GAAGGAGAAC CTCAACCAAC CAAAAAAGCT TCAGGAATGC TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCTCAG AAGAGAGAGA CCTTACGTGG GGCCGACAGT 
GCATACTACC AGGTTGGGCA GACTGGCAAG GAGGGGATGG AGAGTCAAGG CCTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGACACACAG AAGAAACAAC TCACAAATCC CCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGTCGAC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GGGAAGTGAT TCTGGGCAGC AGTGAGGAAG ATTTCATTCG TTCCCTTTCT 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACAGAA 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCGCACT ACTTCAATTA TATCACCAAT GCTGTTCAAC AGAAGAGGCC CACTGCTTTG 
GCTAAAATTC TTGGTGTGTA CAGAATTGGT TACAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTGAAAACTG ACACTGGGAA AGAGAGCTGT 
GACGTGGTTC TGCTGGATGA GAACCTCCTG AAGATGGTTC GAGACAACCC TCTGTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCATA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTTTGCTG GTTGGTCGAG ATGACACTAG CAATGAGCTG 
GTTGTTGGGA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT CGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACCGTGGT CTCTCCAGAG 
TTATACAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTCAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226917.1
CDS	270..6656
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226855.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226855.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGGG TAGAAGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG GGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCTTA CTCCAGCAGC TGCTTCAAAA TGAGTCATTG 
TCATCATCTT GGAGGGACAT CATTGTGTCA CTAGTTTGTC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCCGTG GTTGTCAATG GCTTTGTTTG TACCAAAAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACTT GCATTGACCC CATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTCGTTG AGAAAACAGT GTCTCGGATT GCTCAAGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGA 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACCTGGGCAC TTGCCACAAG 
TTTTATATGC AGCTCTTTCA GTTGCCTAAC GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTGCAC AGCACCTCGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCCTTTC TCATGGATGA GTTTGCTATG CCTCCAACGT TAACACAAAG CCCTTCGTTC 
CATTTTCTGG CTGAGGGAAG AGGGGAAGAG GGAGCTTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCCC AGGATCCTGA GTGCCCCTTT GATGCCCTGT CTTCTGATGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCC TGAGAAGGGT GAGCTGGACA ATAAAAGTGT CCCACAGGCT 
GTTGCCTCCT TGAAGCATCA AGATTACACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CACTGGTACC CGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAGATG AACAGCCAGA GACATCACAG CAAACGGTTG AGCAACAGGA CCCCAAAAGC 
CAGATGAGAG CTTTTAGAGA CCCTCTACAG GATGACACTG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TACTCCTTGA CATTTAAACA GGAATTAAAA 
GACGTCATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GGTGCTCAAC TCGAGATTAC TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAATAAAG AGGTGAAGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGAGCATGAA TGGCAATGTT CAGACCAAGT CCATACAAGT CTTACCTTCA 
CATGAGCTAG TGAGCACCAG GATCGCTGAA CATCTGGGGG ACAGCCAGAG CTTGGGTAGA 
ATGCTAGCTG ACTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATACATC ATGTACTTCA GGGAGCAAAT CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT TCCAAGTGAT GTCTTATGGC CAACAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGCGTG CTCTTCAGCA GTTCTTCTGC CCAGTCCAGC 
AATGCGCCCA GTGCTTGTGT CAGCCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCT 
AGCATGTTCT GTGATACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATATT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CGATGTCATT TGCAAAATAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACTCGCAGAG CCAATGCTGA GCCCTGCGGT CACTCCATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ATCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTG 
TGTGTTCCAC TGCCAAAAAT ATTCATTAAG CGCCAAGCCC CGCTGAAGGT GTCTCTTCTT 
CAGGACCTCA AGGACTTCTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAAAGA 
CTTGCGTCCT TGAAAACAGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAGGATATC 
TTTGCACAAA AGGAGATGGA GGAGGGTGAG TTTAAGAGCT GGACTGAGAA GATGCAGGCG 
CGGCTCATGT CCTCGTGTGT GGACACCCCG CAGCAACTGC AGTCCGTTCT CGAGTCACTC 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGCG CTCCAAGCGT GGAACAGCAG GTTACAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGGAAGAGG CCTTCGGTTC CTCCCAGTCC TGGAAGACTG 
AGACAAGGAG AAGAGAGCAA GATAAGTGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ACGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG TCAGAGCTCC 
ACGAGCTCCA CCCATCTCCA GCTGCCCACT CCTCCTGAGG CCCTGGCTGA GCAGTTAGTG 
GGAGGGCCTT CTGACCTGGA CACAGCCAGT GGCTCTGAAG ATGTATTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA TAATCCCATT CCATTTCCTT TCGATCCAGA TAAACATTAT 
TTAATGTATG AACACGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCA 
ACTCTGTGGA ACAATGCTGA AGAAGGGCTT CCAACCAATA GTGCTTTAGA TAACAGACCA 
AAAAGTAGCA GTCCTATTAG ATTACCTGAA GTCAGTGGAG GACAGACAAA CCGTACAGTA 
GAAGGAGAAC CTCAACCAAC CAAAAAAGCT TCAGGAATGC TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GCCCTGATCT GTCTTCTCAG AAGAGAGAGA CCTTACGTGG GGCCGACAGT 
GCATACTACC AGGTTGGGCA GACTGGCAAG GAGGGGATGG AGAGTCAAGG CCTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGACACACAG AAGAAACAAC TCACAAATCC CCATGTGGAA 
CTTCAATTTT CTGACGCTAA CGCCAAGTTT TACTGTCGAC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GGGAAGTGAT TCTGGGCAGC AGTGAGGAAG ATTTCATTCG TTCCCTTTCT 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACAGAA 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCGCACT ACTTCAATTA TATCACCAAT GCTGTTCAAC AGAAGAGGCC CACTGCTTTG 
GCTAAAATTC TTGGTGTGTA CAGAATTGGT TACAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTGAAAACTG ACACTGGGAA AGAGAGCTGT 
GACGTGGTTC TGCTGGATGA GAACCTCCTG AAGATGGTTC GAGACAACCC TCTGTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCATA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTTTGCTG GTTGGTCGAG ATGACACTAG CAATGAGCTG 
GTTGTTGGGA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT CGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACCGTGGT CTCTCCAGAG 
TTATACAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTCAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245073.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245135.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

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CloneID	ORa18423
Clone ID Related Accession (Same CDS sequence)	XM_006245073.2 , XM_006226856.2
Accession Version	XM_006245073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006245073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTCCA GCTTCGAAGC CTCAGCACAG TGTTGAAACG CCTCAAGGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAGT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACCACCT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGGGCATGC ACATACTGTA GAAAAATAGC CTTAAGTTAC 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAGGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGTGAA CCTCGGACCC CTGTTGGGAG TAGGAAAGCC 
AGTCGTAACA TCTTCTTAGA GGATGACTTA GCCTGGCAAA GTTTGATTCA TCCGGACTCC 
TCGAGTAGTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTGAGTCCC CAGGCTAACC GAAACTACAT CAGGACAGAG 
ACTACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCATACCAG TGGGATGGAA TTTCAGGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATT 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TCGATGGACG TTGGTTGGAT 
TGTGTCAGTC ATCATGACCA GCTTTTCAGG GACGAGTATG CATTGTATAG ACCACTGCAG 
AGTACTGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CGGTGAACTC TGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGGGTAG AAGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGGGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCTTACTC 
CAGCAGCTGC TTCAAAATGA GTCATTGTCA TCATCTTGGA GGGACATCAT TGTGTCACTA 
GTTTGTCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCCGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAAAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACTTGCA TTGACCCCAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTCGTTGAGA AAACAGTGTC TCGGATTGCT 
CAAGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGAGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACC TGGGCACTTG CCACAAGTTT TATATGCAGC TCTTTCAGTT GCCTAACGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTGCACAGC ACCTCGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCCTTTCTCA TGGATGAGTT TGCTATGCCT 
CCAACGTTAA CACAAAGCCC TTCGTTCCAT TTTCTGGCTG AGGGAAGAGG GGAAGAGGGA 
GCTTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCCCAGG ATCCTGAGTG CCCCTTTGAT 
GCCCTGTCTT CTGATGATAG CACTTTGTTG GAATCAAGGA CTGTGCCTGA GAAGGGTGAG 
CTGGACAATA AAAGTGTCCC ACAGGCTGTT GCCTCCTTGA AGCATCAAGA TTACACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAC TGGTACCCGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAGATGAAC AGCCAGAGAC ATCACAGCAA 
ACGGTTGAGC AACAGGACCC CAAAAGCCAG ATGAGAGCTT TTAGAGACCC TCTACAGGAT 
GACACTGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATAAACG AAAGACTTAC 
TCCTTGACAT TTAAACAGGA ATTAAAAGAC GTCATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGGT GCTCAACTCG AGATTACTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AATAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGA GCATGAATGG CAATGTTCAG 
ACCAAGTCCA TACAAGTCTT ACCTTCACAT GAGCTAGTGA GCACCAGGAT CGCTGAACAT 
CTGGGGGACA GCCAGAGCTT GGGTAGAATG CTAGCTGACT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATACATCATG TACTTCAGGG 
AGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTC 
TTATGGCCAA CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGCGTGCTC 
TTCAGCAGTT CTTCTGCCCA GTCCAGCAAT GCGCCCAGTG CTTGTGTCAG CCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCTAGC ATGTTCTGTG ATACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TCCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCGA TGTCATTTGC AAAATACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACT CGCAGAGCCA ATGCTGAGCC CTGCGGTCAC 
TCCATCCACC ATGATTATCA CCAGTATTTC TCTTATAATC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTGTGT GTTCCACTGC CAAAAATATT CATTAAGCGC 
CAAGCCCCGC TGAAGGTGTC TCTTCTTCAG GACCTCAAGG ACTTCTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAAAGACTT GCGTCCTTGA AAACAGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA GGATATCTTT GCACAAAAGG AGATGGAGGA GGGTGAGTTT 
AAGAGCTGGA CTGAGAAGAT GCAGGCGCGG CTCATGTCCT CGTGTGTGGA CACCCCGCAG 
CAACTGCAGT CCGTTCTCGA GTCACTCATT GCCAAGAAGC AAAGCCTCTG TGAGGCGCTC 
CAAGCGTGGA ACAGCAGGTT ACAGGACCTC TTCCAGCAGG AAAAAGGTAG GAAGAGGCCT 
TCGGTTCCTC CCAGTCCTGG AAGACTGAGA CAAGGAGAAG AGAGCAAGAT AAGTGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAACG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGTCA GAGCTCCACG AGCTCCACCC ATCTCCAGCT GCCCACTCCT 
CCTGAGGCCC TGGCTGAGCA GTTAGTGGGA GGGCCTTCTG ACCTGGACAC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG GCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTATAA TCCCATTCCA 
TTTCCTTTCG ATCCAGATAA ACATTATTTA ATGTATGAAC ACGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGTGGAACA ATGCTGAAGA AGGGCTTCCA 
ACCAATAGTG CTTTAGATAA CAGACCAAAA AGTAGCAGTC CTATTAGATT ACCTGAAGTC 
AGTGGAGGAC AGACAAACCG TACAGTAGAA GGAGAACCTC AACCAACCAA AAAAGCTTCA 
GGAATGCTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCTCAGAAG 
AGAGAGACCT TACGTGGGGC CGACAGTGCA TACTACCAGG TTGGGCAGAC TGGCAAGGAG 
GGGATGGAGA GTCAAGGCCT TGAGCCTCAA GATGAAGTAG ATGGAGGAGA CACACAGAAG 
AAACAACTCA CAAATCCCCA TGTGGAACTT CAATTTTCTG ACGCTAACGC CAAGTTTTAC 
TGTCGACTGT ACTACGCGGG AGAGTTCCAT AAGATGCGGG AAGTGATTCT GGGCAGCAGT 
GAGGAAGATT TCATTCGTTC CCTTTCTCAC TCATCACCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACAGAAGAT GATAGATTTA TTTTGAAGCA AATGCCTCGT 
CTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCGCACTACT TCAATTATAT CACCAATGCT 
GTTCAACAGA AGAGGCCCAC TGCTTTGGCT AAAATTCTTG GTGTGTACAG AATTGGTTAC 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTG 
AAAACTGACA CTGGGAAAGA GAGCTGTGAC GTGGTTCTGC TGGATGAGAA CCTCCTGAAG 
ATGGTTCGAG ACAACCCTCT GTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCATAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TTTGCTGGTT 
GGTCGAGATG ACACTAGCAA TGAGCTGGTT GTTGGGATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTCGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CCGTGGTCTC TCCAGAGTTA TACAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTCAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245135.1
CDS	270..6623
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245073.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245073.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTCCA GCTTCGAAGC CTCAGCACAG TGTTGAAACG CCTCAAGGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAGT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACCACCT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGGGCATGC ACATACTGTA GAAAAATAGC CTTAAGTTAC 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAGGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGTGAA CCTCGGACCC CTGTTGGGAG TAGGAAAGCC 
AGTCGTAACA TCTTCTTAGA GGATGACTTA GCCTGGCAAA GTTTGATTCA TCCGGACTCC 
TCGAGTAGTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTGAGTCCC CAGGCTAACC GAAACTACAT CAGGACAGAG 
ACTACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCATACCAG TGGGATGGAA TTTCAGGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATT 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TCGATGGACG TTGGTTGGAT 
TGTGTCAGTC ATCATGACCA GCTTTTCAGG GACGAGTATG CATTGTATAG ACCACTGCAG 
AGTACTGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CGGTGAACTC TGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGGGTAG AAGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGGGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCTTACTC 
CAGCAGCTGC TTCAAAATGA GTCATTGTCA TCATCTTGGA GGGACATCAT TGTGTCACTA 
GTTTGTCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCCGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAAAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACTTGCA TTGACCCCAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTCGTTGAGA AAACAGTGTC TCGGATTGCT 
CAAGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGAGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACC TGGGCACTTG CCACAAGTTT TATATGCAGC TCTTTCAGTT GCCTAACGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTGCACAGC ACCTCGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCCTTTCTCA TGGATGAGTT TGCTATGCCT 
CCAACGTTAA CACAAAGCCC TTCGTTCCAT TTTCTGGCTG AGGGAAGAGG GGAAGAGGGA 
GCTTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCCCAGG ATCCTGAGTG CCCCTTTGAT 
GCCCTGTCTT CTGATGATAG CACTTTGTTG GAATCAAGGA CTGTGCCTGA GAAGGGTGAG 
CTGGACAATA AAAGTGTCCC ACAGGCTGTT GCCTCCTTGA AGCATCAAGA TTACACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAC TGGTACCCGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAGATGAAC AGCCAGAGAC ATCACAGCAA 
ACGGTTGAGC AACAGGACCC CAAAAGCCAG ATGAGAGCTT TTAGAGACCC TCTACAGGAT 
GACACTGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATAAACG AAAGACTTAC 
TCCTTGACAT TTAAACAGGA ATTAAAAGAC GTCATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGGT GCTCAACTCG AGATTACTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AATAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGA GCATGAATGG CAATGTTCAG 
ACCAAGTCCA TACAAGTCTT ACCTTCACAT GAGCTAGTGA GCACCAGGAT CGCTGAACAT 
CTGGGGGACA GCCAGAGCTT GGGTAGAATG CTAGCTGACT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATACATCATG TACTTCAGGG 
AGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTC 
TTATGGCCAA CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGCGTGCTC 
TTCAGCAGTT CTTCTGCCCA GTCCAGCAAT GCGCCCAGTG CTTGTGTCAG CCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCTAGC ATGTTCTGTG ATACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TCCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCGA TGTCATTTGC AAAATACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACT CGCAGAGCCA ATGCTGAGCC CTGCGGTCAC 
TCCATCCACC ATGATTATCA CCAGTATTTC TCTTATAATC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTGTGT GTTCCACTGC CAAAAATATT CATTAAGCGC 
CAAGCCCCGC TGAAGGTGTC TCTTCTTCAG GACCTCAAGG ACTTCTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAAAGACTT GCGTCCTTGA AAACAGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA GGATATCTTT GCACAAAAGG AGATGGAGGA GGGTGAGTTT 
AAGAGCTGGA CTGAGAAGAT GCAGGCGCGG CTCATGTCCT CGTGTGTGGA CACCCCGCAG 
CAACTGCAGT CCGTTCTCGA GTCACTCATT GCCAAGAAGC AAAGCCTCTG TGAGGCGCTC 
CAAGCGTGGA ACAGCAGGTT ACAGGACCTC TTCCAGCAGG AAAAAGGTAG GAAGAGGCCT 
TCGGTTCCTC CCAGTCCTGG AAGACTGAGA CAAGGAGAAG AGAGCAAGAT AAGTGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAACG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGTCA GAGCTCCACG AGCTCCACCC ATCTCCAGCT GCCCACTCCT 
CCTGAGGCCC TGGCTGAGCA GTTAGTGGGA GGGCCTTCTG ACCTGGACAC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG GCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTATAA TCCCATTCCA 
TTTCCTTTCG ATCCAGATAA ACATTATTTA ATGTATGAAC ACGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGTGGAACA ATGCTGAAGA AGGGCTTCCA 
ACCAATAGTG CTTTAGATAA CAGACCAAAA AGTAGCAGTC CTATTAGATT ACCTGAAGTC 
AGTGGAGGAC AGACAAACCG TACAGTAGAA GGAGAACCTC AACCAACCAA AAAAGCTTCA 
GGAATGCTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCTCAGAAG 
AGAGAGACCT TACGTGGGGC CGACAGTGCA TACTACCAGG TTGGGCAGAC TGGCAAGGAG 
GGGATGGAGA GTCAAGGCCT TGAGCCTCAA GATGAAGTAG ATGGAGGAGA CACACAGAAG 
AAACAACTCA CAAATCCCCA TGTGGAACTT CAATTTTCTG ACGCTAACGC CAAGTTTTAC 
TGTCGACTGT ACTACGCGGG AGAGTTCCAT AAGATGCGGG AAGTGATTCT GGGCAGCAGT 
GAGGAAGATT TCATTCGTTC CCTTTCTCAC TCATCACCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACAGAAGAT GATAGATTTA TTTTGAAGCA AATGCCTCGT 
CTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCGCACTACT TCAATTATAT CACCAATGCT 
GTTCAACAGA AGAGGCCCAC TGCTTTGGCT AAAATTCTTG GTGTGTACAG AATTGGTTAC 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTG 
AAAACTGACA CTGGGAAAGA GAGCTGTGAC GTGGTTCTGC TGGATGAGAA CCTCCTGAAG 
ATGGTTCGAG ACAACCCTCT GTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCATAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TTTGCTGGTT 
GGTCGAGATG ACACTAGCAA TGAGCTGGTT GTTGGGATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTCGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CCGTGGTCTC TCCAGAGTTA TACAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTCAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226856.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226918.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

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CloneID	ORa18423
Clone ID Related Accession (Same CDS sequence)	XM_006245073.2 , XM_006226856.2
Accession Version	XM_006226856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6354bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006226856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTCCA GCTTCGAAGC CTCAGCACAG TGTTGAAACG CCTCAAGGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAGT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACCACCT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGGGCATGC ACATACTGTA GAAAAATAGC CTTAAGTTAC 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAGGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGTGAA CCTCGGACCC CTGTTGGGAG TAGGAAAGCC 
AGTCGTAACA TCTTCTTAGA GGATGACTTA GCCTGGCAAA GTTTGATTCA TCCGGACTCC 
TCGAGTAGTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTGAGTCCC CAGGCTAACC GAAACTACAT CAGGACAGAG 
ACTACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCATACCAG TGGGATGGAA TTTCAGGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATT 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TCGATGGACG TTGGTTGGAT 
TGTGTCAGTC ATCATGACCA GCTTTTCAGG GACGAGTATG CATTGTATAG ACCACTGCAG 
AGTACTGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CGGTGAACTC TGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGGGTAG AAGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGGGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCTTACTC 
CAGCAGCTGC TTCAAAATGA GTCATTGTCA TCATCTTGGA GGGACATCAT TGTGTCACTA 
GTTTGTCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCCGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAAAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACTTGCA TTGACCCCAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTCGTTGAGA AAACAGTGTC TCGGATTGCT 
CAAGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGAGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACC TGGGCACTTG CCACAAGTTT TATATGCAGC TCTTTCAGTT GCCTAACGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTGCACAGC ACCTCGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCCTTTCTCA TGGATGAGTT TGCTATGCCT 
CCAACGTTAA CACAAAGCCC TTCGTTCCAT TTTCTGGCTG AGGGAAGAGG GGAAGAGGGA 
GCTTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCCCAGG ATCCTGAGTG CCCCTTTGAT 
GCCCTGTCTT CTGATGATAG CACTTTGTTG GAATCAAGGA CTGTGCCTGA GAAGGGTGAG 
CTGGACAATA AAAGTGTCCC ACAGGCTGTT GCCTCCTTGA AGCATCAAGA TTACACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAC TGGTACCCGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAGATGAAC AGCCAGAGAC ATCACAGCAA 
ACGGTTGAGC AACAGGACCC CAAAAGCCAG ATGAGAGCTT TTAGAGACCC TCTACAGGAT 
GACACTGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATAAACG AAAGACTTAC 
TCCTTGACAT TTAAACAGGA ATTAAAAGAC GTCATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGGT GCTCAACTCG AGATTACTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AATAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGA GCATGAATGG CAATGTTCAG 
ACCAAGTCCA TACAAGTCTT ACCTTCACAT GAGCTAGTGA GCACCAGGAT CGCTGAACAT 
CTGGGGGACA GCCAGAGCTT GGGTAGAATG CTAGCTGACT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATACATCATG TACTTCAGGG 
AGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTC 
TTATGGCCAA CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGCGTGCTC 
TTCAGCAGTT CTTCTGCCCA GTCCAGCAAT GCGCCCAGTG CTTGTGTCAG CCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCTAGC ATGTTCTGTG ATACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TCCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCGA TGTCATTTGC AAAATACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACT CGCAGAGCCA ATGCTGAGCC CTGCGGTCAC 
TCCATCCACC ATGATTATCA CCAGTATTTC TCTTATAATC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTGTGT GTTCCACTGC CAAAAATATT CATTAAGCGC 
CAAGCCCCGC TGAAGGTGTC TCTTCTTCAG GACCTCAAGG ACTTCTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAAAGACTT GCGTCCTTGA AAACAGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA GGATATCTTT GCACAAAAGG AGATGGAGGA GGGTGAGTTT 
AAGAGCTGGA CTGAGAAGAT GCAGGCGCGG CTCATGTCCT CGTGTGTGGA CACCCCGCAG 
CAACTGCAGT CCGTTCTCGA GTCACTCATT GCCAAGAAGC AAAGCCTCTG TGAGGCGCTC 
CAAGCGTGGA ACAGCAGGTT ACAGGACCTC TTCCAGCAGG AAAAAGGTAG GAAGAGGCCT 
TCGGTTCCTC CCAGTCCTGG AAGACTGAGA CAAGGAGAAG AGAGCAAGAT AAGTGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAACG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGTCA GAGCTCCACG AGCTCCACCC ATCTCCAGCT GCCCACTCCT 
CCTGAGGCCC TGGCTGAGCA GTTAGTGGGA GGGCCTTCTG ACCTGGACAC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG GCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTATAA TCCCATTCCA 
TTTCCTTTCG ATCCAGATAA ACATTATTTA ATGTATGAAC ACGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGTGGAACA ATGCTGAAGA AGGGCTTCCA 
ACCAATAGTG CTTTAGATAA CAGACCAAAA AGTAGCAGTC CTATTAGATT ACCTGAAGTC 
AGTGGAGGAC AGACAAACCG TACAGTAGAA GGAGAACCTC AACCAACCAA AAAAGCTTCA 
GGAATGCTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCTCAGAAG 
AGAGAGACCT TACGTGGGGC CGACAGTGCA TACTACCAGG TTGGGCAGAC TGGCAAGGAG 
GGGATGGAGA GTCAAGGCCT TGAGCCTCAA GATGAAGTAG ATGGAGGAGA CACACAGAAG 
AAACAACTCA CAAATCCCCA TGTGGAACTT CAATTTTCTG ACGCTAACGC CAAGTTTTAC 
TGTCGACTGT ACTACGCGGG AGAGTTCCAT AAGATGCGGG AAGTGATTCT GGGCAGCAGT 
GAGGAAGATT TCATTCGTTC CCTTTCTCAC TCATCACCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACAGAAGAT GATAGATTTA TTTTGAAGCA AATGCCTCGT 
CTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCGCACTACT TCAATTATAT CACCAATGCT 
GTTCAACAGA AGAGGCCCAC TGCTTTGGCT AAAATTCTTG GTGTGTACAG AATTGGTTAC 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTG 
AAAACTGACA CTGGGAAAGA GAGCTGTGAC GTGGTTCTGC TGGATGAGAA CCTCCTGAAG 
ATGGTTCGAG ACAACCCTCT GTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCATAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TTTGCTGGTT 
GGTCGAGATG ACACTAGCAA TGAGCTGGTT GTTGGGATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTCGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CCGTGGTCTC TCCAGAGTTA TACAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTCAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226918.1
CDS	270..6623
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226856.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226856.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTCCA GCTTCGAAGC CTCAGCACAG TGTTGAAACG CCTCAAGGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAGT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACCACCT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGGGCATGC ACATACTGTA GAAAAATAGC CTTAAGTTAC 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAGGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGTGAA CCTCGGACCC CTGTTGGGAG TAGGAAAGCC 
AGTCGTAACA TCTTCTTAGA GGATGACTTA GCCTGGCAAA GTTTGATTCA TCCGGACTCC 
TCGAGTAGTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGAAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTGAGTCCC CAGGCTAACC GAAACTACAT CAGGACAGAG 
ACTACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCATACCAG TGGGATGGAA TTTCAGGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATT 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TCGATGGACG TTGGTTGGAT 
TGTGTCAGTC ATCATGACCA GCTTTTCAGG GACGAGTATG CATTGTATAG ACCACTGCAG 
AGTACTGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CGGTGAACTC TGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGGGTAG AAGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGGGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCTTACTC 
CAGCAGCTGC TTCAAAATGA GTCATTGTCA TCATCTTGGA GGGACATCAT TGTGTCACTA 
GTTTGTCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCCGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAAAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACTTGCA TTGACCCCAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTCGTTGAGA AAACAGTGTC TCGGATTGCT 
CAAGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGAGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACC TGGGCACTTG CCACAAGTTT TATATGCAGC TCTTTCAGTT GCCTAACGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTGCACAGC ACCTCGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCCTTTCTCA TGGATGAGTT TGCTATGCCT 
CCAACGTTAA CACAAAGCCC TTCGTTCCAT TTTCTGGCTG AGGGAAGAGG GGAAGAGGGA 
GCTTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCCCAGG ATCCTGAGTG CCCCTTTGAT 
GCCCTGTCTT CTGATGATAG CACTTTGTTG GAATCAAGGA CTGTGCCTGA GAAGGGTGAG 
CTGGACAATA AAAGTGTCCC ACAGGCTGTT GCCTCCTTGA AGCATCAAGA TTACACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAC TGGTACCCGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAGATGAAC AGCCAGAGAC ATCACAGCAA 
ACGGTTGAGC AACAGGACCC CAAAAGCCAG ATGAGAGCTT TTAGAGACCC TCTACAGGAT 
GACACTGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATAAACG AAAGACTTAC 
TCCTTGACAT TTAAACAGGA ATTAAAAGAC GTCATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGGT GCTCAACTCG AGATTACTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AATAAAGAGG TGAAGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGA GCATGAATGG CAATGTTCAG 
ACCAAGTCCA TACAAGTCTT ACCTTCACAT GAGCTAGTGA GCACCAGGAT CGCTGAACAT 
CTGGGGGACA GCCAGAGCTT GGGTAGAATG CTAGCTGACT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATACATCATG TACTTCAGGG 
AGCAAATCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATTCC AAGTGATGTC 
TTATGGCCAA CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGCGTGCTC 
TTCAGCAGTT CTTCTGCCCA GTCCAGCAAT GCGCCCAGTG CTTGTGTCAG CCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCTAGC ATGTTCTGTG ATACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TCCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATATTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCGA TGTCATTTGC AAAATACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACT CGCAGAGCCA ATGCTGAGCC CTGCGGTCAC 
TCCATCCACC ATGATTATCA CCAGTATTTC TCTTATAATC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTGTGT GTTCCACTGC CAAAAATATT CATTAAGCGC 
CAAGCCCCGC TGAAGGTGTC TCTTCTTCAG GACCTCAAGG ACTTCTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAAAGACTT GCGTCCTTGA AAACAGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA GGATATCTTT GCACAAAAGG AGATGGAGGA GGGTGAGTTT 
AAGAGCTGGA CTGAGAAGAT GCAGGCGCGG CTCATGTCCT CGTGTGTGGA CACCCCGCAG 
CAACTGCAGT CCGTTCTCGA GTCACTCATT GCCAAGAAGC AAAGCCTCTG TGAGGCGCTC 
CAAGCGTGGA ACAGCAGGTT ACAGGACCTC TTCCAGCAGG AAAAAGGTAG GAAGAGGCCT 
TCGGTTCCTC CCAGTCCTGG AAGACTGAGA CAAGGAGAAG AGAGCAAGAT AAGTGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAACG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGTCA GAGCTCCACG AGCTCCACCC ATCTCCAGCT GCCCACTCCT 
CCTGAGGCCC TGGCTGAGCA GTTAGTGGGA GGGCCTTCTG ACCTGGACAC AGCCAGTGGC 
TCTGAAGATG TATTTGATGG GCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTATAA TCCCATTCCA 
TTTCCTTTCG ATCCAGATAA ACATTATTTA ATGTATGAAC ACGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCAACT CTGTGGAACA ATGCTGAAGA AGGGCTTCCA 
ACCAATAGTG CTTTAGATAA CAGACCAAAA AGTAGCAGTC CTATTAGATT ACCTGAAGTC 
AGTGGAGGAC AGACAAACCG TACAGTAGAA GGAGAACCTC AACCAACCAA AAAAGCTTCA 
GGAATGCTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGCC CTGATCTGTC TTCTCAGAAG 
AGAGAGACCT TACGTGGGGC CGACAGTGCA TACTACCAGG TTGGGCAGAC TGGCAAGGAG 
GGGATGGAGA GTCAAGGCCT TGAGCCTCAA GATGAAGTAG ATGGAGGAGA CACACAGAAG 
AAACAACTCA CAAATCCCCA TGTGGAACTT CAATTTTCTG ACGCTAACGC CAAGTTTTAC 
TGTCGACTGT ACTACGCGGG AGAGTTCCAT AAGATGCGGG AAGTGATTCT GGGCAGCAGT 
GAGGAAGATT TCATTCGTTC CCTTTCTCAC TCATCACCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACAGAAGAT GATAGATTTA TTTTGAAGCA AATGCCTCGT 
CTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCGCACTACT TCAATTATAT CACCAATGCT 
GTTCAACAGA AGAGGCCCAC TGCTTTGGCT AAAATTCTTG GTGTGTACAG AATTGGTTAC 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTCATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTG 
AAAACTGACA CTGGGAAAGA GAGCTGTGAC GTGGTTCTGC TGGATGAGAA CCTCCTGAAG 
ATGGTTCGAG ACAACCCTCT GTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCATAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TTTGCTGGTT 
GGTCGAGATG ACACTAGCAA TGAGCTGGTT GTTGGGATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTCGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CCGTGGTCTC TCCAGAGTTA TACAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTCAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226860.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226922.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006226860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226922.1
CDS	139..6468
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226860.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226860.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245076.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245138.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006245076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245138.1
CDS	139..6468
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245076.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245076.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226857.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226919.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006226857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226919.1
CDS	194..6523
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226857.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226857.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245077.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245139.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006245077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245139.1
CDS	169..6498
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245077.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245077.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226859.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226921.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006226859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226921.1
CDS	169..6498
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226859.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226859.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245074.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245136.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

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CloneID	ORa18424
Clone ID Related Accession (Same CDS sequence)	XM_006226860.3 , XM_006245076.3 , XM_006226857.3 , XM_006245077.3 , XM_006226859.3 , XM_006245074.3
Accession Version	XM_006245074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245136.1
CDS	194..6523
Translation	MATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245074.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245074.3

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GGGTAGAAGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGGGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC TTACTCCAGC AGCTGCTTCA AAATGAGTCA 
TTGTCATCAT CTTGGAGGGA CATCATTGTG TCACTAGTTT GTCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCC GTGGTTGTCA ATGGCTTTGT TTGTACCAAA 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CTTGCATTGA CCCCATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTCG TTGAGAAAAC AGTGTCTCGG ATTGCTCAAG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGAGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACCTGGG CACTTGCCAC 
AAGTTTTATA TGCAGCTCTT TCAGTTGCCT AACGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTG CACAGCACCT CGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCCT TTCTCATGGA TGAGTTTGCT ATGCCTCCAA CGTTAACACA AAGCCCTTCG 
TTCCATTTTC TGGCTGAGGG AAGAGGGGAA GAGGGAGCTT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CCCAGGATCC TGAGTGCCCC TTTGATGCCC TGTCTTCTGA TGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCCTGAGAAG GGTGAGCTGG ACAATAAAAG TGTCCCACAG 
GCTGTTGCCT CCTTGAAGCA TCAAGATTAC ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCACTGGT ACCCGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAG ATGAACAGCC AGAGACATCA CAGCAAACGG TTGAGCAACA GGACCCCAAA 
AGCCAGATGA GAGCTTTTAG AGACCCTCTA CAGGATGACA CTGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTACTCCT TGACATTTAA ACAGGAATTA 
AAAGACGTCA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGGTGCTC AACTCGAGAT TACTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAATA AAGAGGTGAA GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGAGCAT GAATGGCAAT GTTCAGACCA AGTCCATACA AGTCTTACCT 
TCACATGAGC TAGTGAGCAC CAGGATCGCT GAACATCTGG GGGACAGCCA GAGCTTGGGT 
AGAATGCTAG CTGACTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATAC ATCATGTACT TCAGGGAGCA AATCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATTCCAAGT GATGTCTTAT GGCCAACAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGC GTGCTCTTCA GCAGTTCTTC TGCCCAGTCC 
AGCAATGCGC CCAGTGCTTG TGTCAGCCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCTAGCATGT TCTGTGATAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATCCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ATTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCGATGTC ATTTGCAAAA TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACTCGCA GAGCCAATGC TGAGCCCTGC GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAATCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTGTGTGTTC CACTGCCAAA AATATTCATT AAGCGCCAAG CCCCGCTGAA GGTGTCTCTT 
CTTCAGGACC TCAAGGACTT CTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAA 
AGACTTGCGT CCTTGAAAAC AGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAGGAT 
ATCTTTGCAC AAAAGGAGAT GGAGGAGGGT GAGTTTAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCA TGTCCTCGTG TGTGGACACC CCGCAGCAAC TGCAGTCCGT TCTCGAGTCA 
CTCATTGCCA AGAAGCAAAG CCTCTGTGAG GCGCTCCAAG CGTGGAACAG CAGGTTACAG 
GACCTCTTCC AGCAGGAAAA AGGTAGGAAG AGGCCTTCGG TTCCTCCCAG TCCTGGAAGA 
CTGAGACAAG GAGAAGAGAG CAAGATAAGT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAACGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGTCAGAGC 
TCCACGAGCT CCACCCATCT CCAGCTGCCC ACTCCTCCTG AGGCCCTGGC TGAGCAGTTA 
GTGGGAGGGC CTTCTGACCT GGACACAGCC AGTGGCTCTG AAGATGTATT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTATAATCCC ATTCCATTTC CTTTCGATCC AGATAAACAT 
TATTTAATGT ATGAACACGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCAACTCTGT GGAACAATGC TGAAGAAGGG CTTCCAACCA ATAGTGCTTT AGATAACAGA 
CCAAAAAGTA GCAGTCCTAT TAGATTACCT GAAGTCAGTG GAGGACAGAC AAACCGTACA 
GTAGAAGGAG AACCTCAACC AACCAAAAAA GCTTCAGGAA TGCTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGCCCTGA TCTGTCTTCT CAGAAGAGAG AGACCTTACG TGGGGCCGAC 
AGTGCATACT ACCAGGTTGG GCAGACTGGC AAGGAGGGGA TGGAGAGTCA AGGCCTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGACACA CAGAAGAAAC AACTCACAAA TCCCCATGTG 
GAACTTCAAT TTTCTGACGC TAACGCCAAG TTTTACTGTC GACTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGGGAAGT GATTCTGGGC AGCAGTGAGG AAGATTTCAT TCGTTCCCTT 
TCTCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACA 
GAAGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCGC ACTACTTCAA TTATATCACC AATGCTGTTC AACAGAAGAG GCCCACTGCT 
TTGGCTAAAA TTCTTGGTGT GTACAGAATT GGTTACAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTGAAAA CTGACACTGG GAAAGAGAGC 
TGTGACGTGG TTCTGCTGGA TGAGAACCTC CTGAAGATGG TTCGAGACAA CCCTCTGTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ATAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTTTG CTGGTTGGTC GAGATGACAC TAGCAATGAG 
CTGGTTGTTG GGATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTCGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACCGT GGTCTCTCCA 
GAGTTATACA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT CAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226861.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226923.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

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CloneID	ORa18425
Clone ID Related Accession (Same CDS sequence)	XM_006226861.2 , XM_006245078.2
Accession Version	XM_006226861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006226861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGGGTAGAA GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGGGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCTTACTCCA GCAGCTGCTT 
CAAAATGAGT CATTGTCATC ATCTTGGAGG GACATCATTG TGTCACTAGT TTGTCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CCGTGGTTGT CAATGGCTTT 
GTTTGTACCA AAAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACTTGCATT 
GACCCCATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT CGTTGAGAAA ACAGTGTCTC GGATTGCTCA AGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGAGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACCTG 
GGCACTTGCC ACAAGTTTTA TATGCAGCTC TTTCAGTTGC CTAACGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TGCACAGCAC CTCGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC CTTTCTCATG GATGAGTTTG CTATGCCTCC AACGTTAACA 
CAAAGCCCTT CGTTCCATTT TCTGGCTGAG GGAAGAGGGG AAGAGGGAGC TTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCCCAGGAT CCTGAGTGCC CCTTTGATGC CCTGTCTTCT 
GATGATAGCA CTTTGTTGGA ATCAAGGACT GTGCCTGAGA AGGGTGAGCT GGACAATAAA 
AGTGTCCCAC AGGCTGTTGC CTCCTTGAAG CATCAAGATT ACACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCACTG GTACCCGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AGATGAACAG CCAGAGACAT CACAGCAAAC GGTTGAGCAA 
CAGGACCCCA AAAGCCAGAT GAGAGCTTTT AGAGACCCTC TACAGGATGA CACTGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTACTC CTTGACATTT 
AAACAGGAAT TAAAAGACGT CATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGGTGC TCAACTCGAG ATTACTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA TAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGAGC ATGAATGGCA ATGTTCAGAC CAAGTCCATA 
CAAGTCTTAC CTTCACATGA GCTAGTGAGC ACCAGGATCG CTGAACATCT GGGGGACAGC 
CAGAGCTTGG GTAGAATGCT AGCTGACTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT ACATCATGTA CTTCAGGGAG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTCTT ATGGCCAACA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GCGTGCTCTT CAGCAGTTCT 
TCTGCCCAGT CCAGCAATGC GCCCAGTGCT TGTGTCAGCC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCTAGCAT GTTCTGTGAT ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATC CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCGATG TCATTTGCAA AATACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACTCG CAGAGCCAAT GCTGAGCCCT GCGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC TTATAATCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTGTGTGT TCCACTGCCA AAAATATTCA TTAAGCGCCA AGCCCCGCTG 
AAGGTGTCTC TTCTTCAGGA CCTCAAGGAC TTCTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AAAGACTTGC GTCCTTGAAA ACAGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAGG ATATCTTTGC ACAAAAGGAG ATGGAGGAGG GTGAGTTTAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CATGTCCTCG TGTGTGGACA CCCCGCAGCA ACTGCAGTCC 
GTTCTCGAGT CACTCATTGC CAAGAAGCAA AGCCTCTGTG AGGCGCTCCA AGCGTGGAAC 
AGCAGGTTAC AGGACCTCTT CCAGCAGGAA AAAGGTAGGA AGAGGCCTTC GGTTCCTCCC 
AGTCCTGGAA GACTGAGACA AGGAGAAGAG AGCAAGATAA GTGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAACGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGTCAGA GCTCCACGAG CTCCACCCAT CTCCAGCTGC CCACTCCTCC TGAGGCCCTG 
GCTGAGCAGT TAGTGGGAGG GCCTTCTGAC CTGGACACAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTATAATC CCATTCCATT TCCTTTCGAT 
CCAGATAAAC ATTATTTAAT GTATGAACAC GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GTGGAACAAT GCTGAAGAAG GGCTTCCAAC CAATAGTGCT 
TTAGATAACA GACCAAAAAG TAGCAGTCCT ATTAGATTAC CTGAAGTCAG TGGAGGACAG 
ACAAACCGTA CAGTAGAAGG AGAACCTCAA CCAACCAAAA AAGCTTCAGG AATGCTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CTCAGAAGAG AGAGACCTTA 
CGTGGGGCCG ACAGTGCATA CTACCAGGTT GGGCAGACTG GCAAGGAGGG GATGGAGAGT 
CAAGGCCTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGACA CACAGAAGAA ACAACTCACA 
AATCCCCATG TGGAACTTCA ATTTTCTGAC GCTAACGCCA AGTTTTACTG TCGACTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGGGAA GTGATTCTGG GCAGCAGTGA GGAAGATTTC 
ATTCGTTCCC TTTCTCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CAGAAGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC GCACTACTTC AATTATATCA CCAATGCTGT TCAACAGAAG 
AGGCCCACTG CTTTGGCTAA AATTCTTGGT GTGTACAGAA TTGGTTACAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTGAA AACTGACACT 
GGGAAAGAGA GCTGTGACGT GGTTCTGCTG GATGAGAACC TCCTGAAGAT GGTTCGAGAC 
AACCCTCTGT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCATAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTT TGCTGGTTGG TCGAGATGAC 
ACTAGCAATG AGCTGGTTGT TGGGATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTCGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACC 
GTGGTCTCTC CAGAGTTATA CAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTCAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226923.1
CDS	270..6491
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226861.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226861.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGGGTAGAA GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGGGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCTTACTCCA GCAGCTGCTT 
CAAAATGAGT CATTGTCATC ATCTTGGAGG GACATCATTG TGTCACTAGT TTGTCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CCGTGGTTGT CAATGGCTTT 
GTTTGTACCA AAAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACTTGCATT 
GACCCCATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT CGTTGAGAAA ACAGTGTCTC GGATTGCTCA AGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGAGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACCTG 
GGCACTTGCC ACAAGTTTTA TATGCAGCTC TTTCAGTTGC CTAACGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TGCACAGCAC CTCGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC CTTTCTCATG GATGAGTTTG CTATGCCTCC AACGTTAACA 
CAAAGCCCTT CGTTCCATTT TCTGGCTGAG GGAAGAGGGG AAGAGGGAGC TTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCCCAGGAT CCTGAGTGCC CCTTTGATGC CCTGTCTTCT 
GATGATAGCA CTTTGTTGGA ATCAAGGACT GTGCCTGAGA AGGGTGAGCT GGACAATAAA 
AGTGTCCCAC AGGCTGTTGC CTCCTTGAAG CATCAAGATT ACACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCACTG GTACCCGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AGATGAACAG CCAGAGACAT CACAGCAAAC GGTTGAGCAA 
CAGGACCCCA AAAGCCAGAT GAGAGCTTTT AGAGACCCTC TACAGGATGA CACTGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTACTC CTTGACATTT 
AAACAGGAAT TAAAAGACGT CATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGGTGC TCAACTCGAG ATTACTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA TAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGAGC ATGAATGGCA ATGTTCAGAC CAAGTCCATA 
CAAGTCTTAC CTTCACATGA GCTAGTGAGC ACCAGGATCG CTGAACATCT GGGGGACAGC 
CAGAGCTTGG GTAGAATGCT AGCTGACTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT ACATCATGTA CTTCAGGGAG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTCTT ATGGCCAACA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GCGTGCTCTT CAGCAGTTCT 
TCTGCCCAGT CCAGCAATGC GCCCAGTGCT TGTGTCAGCC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCTAGCAT GTTCTGTGAT ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATC CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCGATG TCATTTGCAA AATACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACTCG CAGAGCCAAT GCTGAGCCCT GCGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC TTATAATCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTGTGTGT TCCACTGCCA AAAATATTCA TTAAGCGCCA AGCCCCGCTG 
AAGGTGTCTC TTCTTCAGGA CCTCAAGGAC TTCTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AAAGACTTGC GTCCTTGAAA ACAGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAGG ATATCTTTGC ACAAAAGGAG ATGGAGGAGG GTGAGTTTAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CATGTCCTCG TGTGTGGACA CCCCGCAGCA ACTGCAGTCC 
GTTCTCGAGT CACTCATTGC CAAGAAGCAA AGCCTCTGTG AGGCGCTCCA AGCGTGGAAC 
AGCAGGTTAC AGGACCTCTT CCAGCAGGAA AAAGGTAGGA AGAGGCCTTC GGTTCCTCCC 
AGTCCTGGAA GACTGAGACA AGGAGAAGAG AGCAAGATAA GTGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAACGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGTCAGA GCTCCACGAG CTCCACCCAT CTCCAGCTGC CCACTCCTCC TGAGGCCCTG 
GCTGAGCAGT TAGTGGGAGG GCCTTCTGAC CTGGACACAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTATAATC CCATTCCATT TCCTTTCGAT 
CCAGATAAAC ATTATTTAAT GTATGAACAC GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GTGGAACAAT GCTGAAGAAG GGCTTCCAAC CAATAGTGCT 
TTAGATAACA GACCAAAAAG TAGCAGTCCT ATTAGATTAC CTGAAGTCAG TGGAGGACAG 
ACAAACCGTA CAGTAGAAGG AGAACCTCAA CCAACCAAAA AAGCTTCAGG AATGCTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CTCAGAAGAG AGAGACCTTA 
CGTGGGGCCG ACAGTGCATA CTACCAGGTT GGGCAGACTG GCAAGGAGGG GATGGAGAGT 
CAAGGCCTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGACA CACAGAAGAA ACAACTCACA 
AATCCCCATG TGGAACTTCA ATTTTCTGAC GCTAACGCCA AGTTTTACTG TCGACTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGGGAA GTGATTCTGG GCAGCAGTGA GGAAGATTTC 
ATTCGTTCCC TTTCTCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CAGAAGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC GCACTACTTC AATTATATCA CCAATGCTGT TCAACAGAAG 
AGGCCCACTG CTTTGGCTAA AATTCTTGGT GTGTACAGAA TTGGTTACAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTGAA AACTGACACT 
GGGAAAGAGA GCTGTGACGT GGTTCTGCTG GATGAGAACC TCCTGAAGAT GGTTCGAGAC 
AACCCTCTGT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCATAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTT TGCTGGTTGG TCGAGATGAC 
ACTAGCAATG AGCTGGTTGT TGGGATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTCGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACC 
GTGGTCTCTC CAGAGTTATA CAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTCAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245078.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245140.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

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CloneID	ORa18425
Clone ID Related Accession (Same CDS sequence)	XM_006226861.2 , XM_006245078.2
Accession Version	XM_006245078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006245078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGGGTAGAA GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGGGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCTTACTCCA GCAGCTGCTT 
CAAAATGAGT CATTGTCATC ATCTTGGAGG GACATCATTG TGTCACTAGT TTGTCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CCGTGGTTGT CAATGGCTTT 
GTTTGTACCA AAAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACTTGCATT 
GACCCCATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT CGTTGAGAAA ACAGTGTCTC GGATTGCTCA AGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGAGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACCTG 
GGCACTTGCC ACAAGTTTTA TATGCAGCTC TTTCAGTTGC CTAACGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TGCACAGCAC CTCGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC CTTTCTCATG GATGAGTTTG CTATGCCTCC AACGTTAACA 
CAAAGCCCTT CGTTCCATTT TCTGGCTGAG GGAAGAGGGG AAGAGGGAGC TTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCCCAGGAT CCTGAGTGCC CCTTTGATGC CCTGTCTTCT 
GATGATAGCA CTTTGTTGGA ATCAAGGACT GTGCCTGAGA AGGGTGAGCT GGACAATAAA 
AGTGTCCCAC AGGCTGTTGC CTCCTTGAAG CATCAAGATT ACACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCACTG GTACCCGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AGATGAACAG CCAGAGACAT CACAGCAAAC GGTTGAGCAA 
CAGGACCCCA AAAGCCAGAT GAGAGCTTTT AGAGACCCTC TACAGGATGA CACTGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTACTC CTTGACATTT 
AAACAGGAAT TAAAAGACGT CATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGGTGC TCAACTCGAG ATTACTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA TAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGAGC ATGAATGGCA ATGTTCAGAC CAAGTCCATA 
CAAGTCTTAC CTTCACATGA GCTAGTGAGC ACCAGGATCG CTGAACATCT GGGGGACAGC 
CAGAGCTTGG GTAGAATGCT AGCTGACTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT ACATCATGTA CTTCAGGGAG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTCTT ATGGCCAACA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GCGTGCTCTT CAGCAGTTCT 
TCTGCCCAGT CCAGCAATGC GCCCAGTGCT TGTGTCAGCC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCTAGCAT GTTCTGTGAT ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATC CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCGATG TCATTTGCAA AATACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACTCG CAGAGCCAAT GCTGAGCCCT GCGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC TTATAATCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTGTGTGT TCCACTGCCA AAAATATTCA TTAAGCGCCA AGCCCCGCTG 
AAGGTGTCTC TTCTTCAGGA CCTCAAGGAC TTCTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AAAGACTTGC GTCCTTGAAA ACAGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAGG ATATCTTTGC ACAAAAGGAG ATGGAGGAGG GTGAGTTTAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CATGTCCTCG TGTGTGGACA CCCCGCAGCA ACTGCAGTCC 
GTTCTCGAGT CACTCATTGC CAAGAAGCAA AGCCTCTGTG AGGCGCTCCA AGCGTGGAAC 
AGCAGGTTAC AGGACCTCTT CCAGCAGGAA AAAGGTAGGA AGAGGCCTTC GGTTCCTCCC 
AGTCCTGGAA GACTGAGACA AGGAGAAGAG AGCAAGATAA GTGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAACGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGTCAGA GCTCCACGAG CTCCACCCAT CTCCAGCTGC CCACTCCTCC TGAGGCCCTG 
GCTGAGCAGT TAGTGGGAGG GCCTTCTGAC CTGGACACAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTATAATC CCATTCCATT TCCTTTCGAT 
CCAGATAAAC ATTATTTAAT GTATGAACAC GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GTGGAACAAT GCTGAAGAAG GGCTTCCAAC CAATAGTGCT 
TTAGATAACA GACCAAAAAG TAGCAGTCCT ATTAGATTAC CTGAAGTCAG TGGAGGACAG 
ACAAACCGTA CAGTAGAAGG AGAACCTCAA CCAACCAAAA AAGCTTCAGG AATGCTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CTCAGAAGAG AGAGACCTTA 
CGTGGGGCCG ACAGTGCATA CTACCAGGTT GGGCAGACTG GCAAGGAGGG GATGGAGAGT 
CAAGGCCTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGACA CACAGAAGAA ACAACTCACA 
AATCCCCATG TGGAACTTCA ATTTTCTGAC GCTAACGCCA AGTTTTACTG TCGACTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGGGAA GTGATTCTGG GCAGCAGTGA GGAAGATTTC 
ATTCGTTCCC TTTCTCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CAGAAGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC GCACTACTTC AATTATATCA CCAATGCTGT TCAACAGAAG 
AGGCCCACTG CTTTGGCTAA AATTCTTGGT GTGTACAGAA TTGGTTACAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTGAA AACTGACACT 
GGGAAAGAGA GCTGTGACGT GGTTCTGCTG GATGAGAACC TCCTGAAGAT GGTTCGAGAC 
AACCCTCTGT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCATAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTT TGCTGGTTGG TCGAGATGAC 
ACTAGCAATG AGCTGGTTGT TGGGATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTCGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACC 
GTGGTCTCTC CAGAGTTATA CAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTCAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245140.1
CDS	270..6491
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245078.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245078.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAT ATTTTGCCAA ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTCCAGCTT 
CGAAGCCTCA GCACAGTGTT GAAACGCCTC AAGGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAGTATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CCACCTTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGG 
GCATGCACAT ACTGTAGAAA AATAGCCTTA AGTTACGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAGG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGTGAACCTC GGACCCCTGT TGGGAGTAGG AAAGCCAGTC GTAACATCTT CTTAGAGGAT 
GACTTAGCCT GGCAAAGTTT GATTCATCCG GACTCCTCGA GTAGTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GAAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTG 
AGTCCCCAGG CTAACCGAAA CTACATCAGG ACAGAGACTA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA TACCAGTGGG 
ATGGAATTTC AGGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATTGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTCGA TGGACGTTGG TTGGATTGTG TCAGTCATCA TGACCAGCTT 
TTCAGGGACG AGTATGCATT GTATAGACCA CTGCAGAGTA CTGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGGGTAGAA GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGGGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCTTACTCCA GCAGCTGCTT 
CAAAATGAGT CATTGTCATC ATCTTGGAGG GACATCATTG TGTCACTAGT TTGTCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CCGTGGTTGT CAATGGCTTT 
GTTTGTACCA AAAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACTTGCATT 
GACCCCATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT CGTTGAGAAA ACAGTGTCTC GGATTGCTCA AGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGAGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACCTG 
GGCACTTGCC ACAAGTTTTA TATGCAGCTC TTTCAGTTGC CTAACGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TGCACAGCAC CTCGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC CTTTCTCATG GATGAGTTTG CTATGCCTCC AACGTTAACA 
CAAAGCCCTT CGTTCCATTT TCTGGCTGAG GGAAGAGGGG AAGAGGGAGC TTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCCCAGGAT CCTGAGTGCC CCTTTGATGC CCTGTCTTCT 
GATGATAGCA CTTTGTTGGA ATCAAGGACT GTGCCTGAGA AGGGTGAGCT GGACAATAAA 
AGTGTCCCAC AGGCTGTTGC CTCCTTGAAG CATCAAGATT ACACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCACTG GTACCCGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AGATGAACAG CCAGAGACAT CACAGCAAAC GGTTGAGCAA 
CAGGACCCCA AAAGCCAGAT GAGAGCTTTT AGAGACCCTC TACAGGATGA CACTGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTACTC CTTGACATTT 
AAACAGGAAT TAAAAGACGT CATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGGTGC TCAACTCGAG ATTACTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA TAAAGAGGTG AAGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGAGC ATGAATGGCA ATGTTCAGAC CAAGTCCATA 
CAAGTCTTAC CTTCACATGA GCTAGTGAGC ACCAGGATCG CTGAACATCT GGGGGACAGC 
CAGAGCTTGG GTAGAATGCT AGCTGACTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT ACATCATGTA CTTCAGGGAG CAAATCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATTCCAA GTGATGTCTT ATGGCCAACA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GCGTGCTCTT CAGCAGTTCT 
TCTGCCCAGT CCAGCAATGC GCCCAGTGCT TGTGTCAGCC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCTAGCAT GTTCTGTGAT ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATC CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATATTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCGATG TCATTTGCAA AATACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACTCG CAGAGCCAAT GCTGAGCCCT GCGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC TTATAATCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTGTGTGT TCCACTGCCA AAAATATTCA TTAAGCGCCA AGCCCCGCTG 
AAGGTGTCTC TTCTTCAGGA CCTCAAGGAC TTCTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AAAGACTTGC GTCCTTGAAA ACAGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAGG ATATCTTTGC ACAAAAGGAG ATGGAGGAGG GTGAGTTTAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CATGTCCTCG TGTGTGGACA CCCCGCAGCA ACTGCAGTCC 
GTTCTCGAGT CACTCATTGC CAAGAAGCAA AGCCTCTGTG AGGCGCTCCA AGCGTGGAAC 
AGCAGGTTAC AGGACCTCTT CCAGCAGGAA AAAGGTAGGA AGAGGCCTTC GGTTCCTCCC 
AGTCCTGGAA GACTGAGACA AGGAGAAGAG AGCAAGATAA GTGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAACGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGTCAGA GCTCCACGAG CTCCACCCAT CTCCAGCTGC CCACTCCTCC TGAGGCCCTG 
GCTGAGCAGT TAGTGGGAGG GCCTTCTGAC CTGGACACAG CCAGTGGCTC TGAAGATGTA 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTATAATC CCATTCCATT TCCTTTCGAT 
CCAGATAAAC ATTATTTAAT GTATGAACAC GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCAACTCT GTGGAACAAT GCTGAAGAAG GGCTTCCAAC CAATAGTGCT 
TTAGATAACA GACCAAAAAG TAGCAGTCCT ATTAGATTAC CTGAAGTCAG TGGAGGACAG 
ACAAACCGTA CAGTAGAAGG AGAACCTCAA CCAACCAAAA AAGCTTCAGG AATGCTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGCCCT GATCTGTCTT CTCAGAAGAG AGAGACCTTA 
CGTGGGGCCG ACAGTGCATA CTACCAGGTT GGGCAGACTG GCAAGGAGGG GATGGAGAGT 
CAAGGCCTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGACA CACAGAAGAA ACAACTCACA 
AATCCCCATG TGGAACTTCA ATTTTCTGAC GCTAACGCCA AGTTTTACTG TCGACTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGGGAA GTGATTCTGG GCAGCAGTGA GGAAGATTTC 
ATTCGTTCCC TTTCTCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CAGAAGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC GCACTACTTC AATTATATCA CCAATGCTGT TCAACAGAAG 
AGGCCCACTG CTTTGGCTAA AATTCTTGGT GTGTACAGAA TTGGTTACAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTGAA AACTGACACT 
GGGAAAGAGA GCTGTGACGT GGTTCTGCTG GATGAGAACC TCCTGAAGAT GGTTCGAGAC 
AACCCTCTGT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCATAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTT TGCTGGTTGG TCGAGATGAC 
ACTAGCAATG AGCTGGTTGT TGGGATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTCGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACC 
GTGGTCTCTC CAGAGTTATA CAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTCAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006226862.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226924.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

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CloneID	ORa18426
Clone ID Related Accession (Same CDS sequence)	XM_006226862.2 , XM_006245079.2
Accession Version	XM_006226862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006226862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG GGTAGAAGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGGGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCT TACTCCAGCA GCTGCTTCAA 
AATGAGTCAT TGTCATCATC TTGGAGGGAC ATCATTGTGT CACTAGTTTG TCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCCG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAAA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC TTGCATTGAC 
CCCATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTCGT TGAGAAAACA GTGTCTCGGA TTGCTCAAGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GAGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACCTGGGC 
ACTTGCCACA AGTTTTATAT GCAGCTCTTT CAGTTGCCTA ACGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTGC ACAGCACCTC GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCCTT TCTCATGGAT GAGTTTGCTA TGCCTCCAAC GTTAACACAA 
AGCCCTTCGT TCCATTTTCT GGCTGAGGGA AGAGGGGAAG AGGGAGCTTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC CCAGGATCCT GAGTGCCCCT TTGATGCCCT GTCTTCTGAT 
GATAGCACTT TGTTGGAATC AAGGACTGTG CCTGAGAAGG GTGAGCTGGA CAATAAAAGT 
GTCCCACAGG CTGTTGCCTC CTTGAAGCAT CAAGATTACA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCACTGGTA CCCGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAGA TGAACAGCCA GAGACATCAC AGCAAACGGT TGAGCAACAG 
GACCCCAAAA GCCAGATGAG AGCTTTTAGA GACCCTCTAC AGGATGACAC TGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTACTCCTT GACATTTAAA 
CAGGAATTAA AAGACGTCAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGGTGCTCA ACTCGAGATT ACTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAATAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGAGCATG AATGGCAATG TTCAGACCAA GTCCATACAA 
GTCTTACCTT CACATGAGCT AGTGAGCACC AGGATCGCTG AACATCTGGG GGACAGCCAG 
AGCTTGGGTA GAATGCTAGC TGACTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATACA TCATGTACTT CAGGGAGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTCTTATG GCCAACAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGCG TGCTCTTCAG CAGTTCTTCT 
GCCCAGTCCA GCAATGCGCC CAGTGCTTGT GTCAGCCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CTAGCATGTT CTGTGATACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATCCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCGATGTCA TTTGCAAAAT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACTCGCAG AGCCAATGCT GAGCCCTGCG GTCACTCCAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAATCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TGTGTGTTCC ACTGCCAAAA ATATTCATTA AGCGCCAAGC CCCGCTGAAG 
GTGTCTCTTC TTCAGGACCT CAAGGACTTC TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAAA GACTTGCGTC CTTGAAAACA GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAGGATA TCTTTGCACA AAAGGAGATG GAGGAGGGTG AGTTTAAGAG CTGGACTGAG 
AAGATGCAGG CGCGGCTCAT GTCCTCGTGT GTGGACACCC CGCAGCAACT GCAGTCCGTT 
CTCGAGTCAC TCATTGCCAA GAAGCAAAGC CTCTGTGAGG CGCTCCAAGC GTGGAACAGC 
AGGTTACAGG ACCTCTTCCA GCAGGAAAAA GGTAGGAAGA GGCCTTCGGT TCCTCCCAGT 
CCTGGAAGAC TGAGACAAGG AGAAGAGAGC AAGATAAGTG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAACGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGTCAGAGCT CCACGAGCTC CACCCATCTC CAGCTGCCCA CTCCTCCTGA GGCCCTGGCT 
GAGCAGTTAG TGGGAGGGCC TTCTGACCTG GACACAGCCA GTGGCTCTGA AGATGTATTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TATAATCCCA TTCCATTTCC TTTCGATCCA 
GATAAACATT ATTTAATGTA TGAACACGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGTG GAACAATGCT GAAGAAGGGC TTCCAACCAA TAGTGCTTTA 
GATAACAGAC CAAAAAGTAG CAGTCCTATT AGATTACCTG AAGTCAGTGG AGGACAGACA 
AACCGTACAG TAGAAGGAGA ACCTCAACCA ACCAAAAAAG CTTCAGGAAT GCTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCTC AGAAGAGAGA GACCTTACGT 
GGGGCCGACA GTGCATACTA CCAGGTTGGG CAGACTGGCA AGGAGGGGAT GGAGAGTCAA 
GGCCTTGAGC CTCAAGATGA AGTAGATGGA GGAGACACAC AGAAGAAACA ACTCACAAAT 
CCCCATGTGG AACTTCAATT TTCTGACGCT AACGCCAAGT TTTACTGTCG ACTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGGGAAGTG ATTCTGGGCA GCAGTGAGGA AGATTTCATT 
CGTTCCCTTT CTCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACAG AAGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCGCA CTACTTCAAT TATATCACCA ATGCTGTTCA ACAGAAGAGG 
CCCACTGCTT TGGCTAAAAT TCTTGGTGTG TACAGAATTG GTTACAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTGAAAAC TGACACTGGG 
AAAGAGAGCT GTGACGTGGT TCTGCTGGAT GAGAACCTCC TGAAGATGGT TCGAGACAAC 
CCTCTGTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA TAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTTTGC TGGTTGGTCG AGATGACACT 
AGCAATGAGC TGGTTGTTGG GATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTCGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACCGTG 
GTCTCTCCAG AGTTATACAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTC AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226924.1
CDS	270..6488
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006226862.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226862.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG GGTAGAAGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGGGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCT TACTCCAGCA GCTGCTTCAA 
AATGAGTCAT TGTCATCATC TTGGAGGGAC ATCATTGTGT CACTAGTTTG TCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCCG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAAA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC TTGCATTGAC 
CCCATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTCGT TGAGAAAACA GTGTCTCGGA TTGCTCAAGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GAGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACCTGGGC 
ACTTGCCACA AGTTTTATAT GCAGCTCTTT CAGTTGCCTA ACGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTGC ACAGCACCTC GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCCTT TCTCATGGAT GAGTTTGCTA TGCCTCCAAC GTTAACACAA 
AGCCCTTCGT TCCATTTTCT GGCTGAGGGA AGAGGGGAAG AGGGAGCTTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC CCAGGATCCT GAGTGCCCCT TTGATGCCCT GTCTTCTGAT 
GATAGCACTT TGTTGGAATC AAGGACTGTG CCTGAGAAGG GTGAGCTGGA CAATAAAAGT 
GTCCCACAGG CTGTTGCCTC CTTGAAGCAT CAAGATTACA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCACTGGTA CCCGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAGA TGAACAGCCA GAGACATCAC AGCAAACGGT TGAGCAACAG 
GACCCCAAAA GCCAGATGAG AGCTTTTAGA GACCCTCTAC AGGATGACAC TGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTACTCCTT GACATTTAAA 
CAGGAATTAA AAGACGTCAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGGTGCTCA ACTCGAGATT ACTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAATAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGAGCATG AATGGCAATG TTCAGACCAA GTCCATACAA 
GTCTTACCTT CACATGAGCT AGTGAGCACC AGGATCGCTG AACATCTGGG GGACAGCCAG 
AGCTTGGGTA GAATGCTAGC TGACTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATACA TCATGTACTT CAGGGAGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTCTTATG GCCAACAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGCG TGCTCTTCAG CAGTTCTTCT 
GCCCAGTCCA GCAATGCGCC CAGTGCTTGT GTCAGCCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CTAGCATGTT CTGTGATACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATCCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCGATGTCA TTTGCAAAAT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACTCGCAG AGCCAATGCT GAGCCCTGCG GTCACTCCAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAATCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TGTGTGTTCC ACTGCCAAAA ATATTCATTA AGCGCCAAGC CCCGCTGAAG 
GTGTCTCTTC TTCAGGACCT CAAGGACTTC TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAAA GACTTGCGTC CTTGAAAACA GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAGGATA TCTTTGCACA AAAGGAGATG GAGGAGGGTG AGTTTAAGAG CTGGACTGAG 
AAGATGCAGG CGCGGCTCAT GTCCTCGTGT GTGGACACCC CGCAGCAACT GCAGTCCGTT 
CTCGAGTCAC TCATTGCCAA GAAGCAAAGC CTCTGTGAGG CGCTCCAAGC GTGGAACAGC 
AGGTTACAGG ACCTCTTCCA GCAGGAAAAA GGTAGGAAGA GGCCTTCGGT TCCTCCCAGT 
CCTGGAAGAC TGAGACAAGG AGAAGAGAGC AAGATAAGTG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAACGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGTCAGAGCT CCACGAGCTC CACCCATCTC CAGCTGCCCA CTCCTCCTGA GGCCCTGGCT 
GAGCAGTTAG TGGGAGGGCC TTCTGACCTG GACACAGCCA GTGGCTCTGA AGATGTATTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TATAATCCCA TTCCATTTCC TTTCGATCCA 
GATAAACATT ATTTAATGTA TGAACACGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGTG GAACAATGCT GAAGAAGGGC TTCCAACCAA TAGTGCTTTA 
GATAACAGAC CAAAAAGTAG CAGTCCTATT AGATTACCTG AAGTCAGTGG AGGACAGACA 
AACCGTACAG TAGAAGGAGA ACCTCAACCA ACCAAAAAAG CTTCAGGAAT GCTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCTC AGAAGAGAGA GACCTTACGT 
GGGGCCGACA GTGCATACTA CCAGGTTGGG CAGACTGGCA AGGAGGGGAT GGAGAGTCAA 
GGCCTTGAGC CTCAAGATGA AGTAGATGGA GGAGACACAC AGAAGAAACA ACTCACAAAT 
CCCCATGTGG AACTTCAATT TTCTGACGCT AACGCCAAGT TTTACTGTCG ACTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGGGAAGTG ATTCTGGGCA GCAGTGAGGA AGATTTCATT 
CGTTCCCTTT CTCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACAG AAGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCGCA CTACTTCAAT TATATCACCA ATGCTGTTCA ACAGAAGAGG 
CCCACTGCTT TGGCTAAAAT TCTTGGTGTG TACAGAATTG GTTACAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTGAAAAC TGACACTGGG 
AAAGAGAGCT GTGACGTGGT TCTGCTGGAT GAGAACCTCC TGAAGATGGT TCGAGACAAC 
CCTCTGTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA TAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTTTGC TGGTTGGTCG AGATGACACT 
AGCAATGAGC TGGTTGTTGG GATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTCGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACCGTG 
GTCTCTCCAG AGTTATACAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTC AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_006245079.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245141.1 Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

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CloneID	ORa18426
Clone ID Related Accession (Same CDS sequence)	XM_006226862.2 , XM_006245079.2
Accession Version	XM_006245079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006245079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG GGTAGAAGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGGGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCT TACTCCAGCA GCTGCTTCAA 
AATGAGTCAT TGTCATCATC TTGGAGGGAC ATCATTGTGT CACTAGTTTG TCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCCG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAAA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC TTGCATTGAC 
CCCATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTCGT TGAGAAAACA GTGTCTCGGA TTGCTCAAGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GAGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACCTGGGC 
ACTTGCCACA AGTTTTATAT GCAGCTCTTT CAGTTGCCTA ACGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTGC ACAGCACCTC GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCCTT TCTCATGGAT GAGTTTGCTA TGCCTCCAAC GTTAACACAA 
AGCCCTTCGT TCCATTTTCT GGCTGAGGGA AGAGGGGAAG AGGGAGCTTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC CCAGGATCCT GAGTGCCCCT TTGATGCCCT GTCTTCTGAT 
GATAGCACTT TGTTGGAATC AAGGACTGTG CCTGAGAAGG GTGAGCTGGA CAATAAAAGT 
GTCCCACAGG CTGTTGCCTC CTTGAAGCAT CAAGATTACA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCACTGGTA CCCGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAGA TGAACAGCCA GAGACATCAC AGCAAACGGT TGAGCAACAG 
GACCCCAAAA GCCAGATGAG AGCTTTTAGA GACCCTCTAC AGGATGACAC TGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTACTCCTT GACATTTAAA 
CAGGAATTAA AAGACGTCAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGGTGCTCA ACTCGAGATT ACTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAATAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGAGCATG AATGGCAATG TTCAGACCAA GTCCATACAA 
GTCTTACCTT CACATGAGCT AGTGAGCACC AGGATCGCTG AACATCTGGG GGACAGCCAG 
AGCTTGGGTA GAATGCTAGC TGACTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATACA TCATGTACTT CAGGGAGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTCTTATG GCCAACAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGCG TGCTCTTCAG CAGTTCTTCT 
GCCCAGTCCA GCAATGCGCC CAGTGCTTGT GTCAGCCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CTAGCATGTT CTGTGATACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATCCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCGATGTCA TTTGCAAAAT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACTCGCAG AGCCAATGCT GAGCCCTGCG GTCACTCCAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAATCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TGTGTGTTCC ACTGCCAAAA ATATTCATTA AGCGCCAAGC CCCGCTGAAG 
GTGTCTCTTC TTCAGGACCT CAAGGACTTC TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAAA GACTTGCGTC CTTGAAAACA GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAGGATA TCTTTGCACA AAAGGAGATG GAGGAGGGTG AGTTTAAGAG CTGGACTGAG 
AAGATGCAGG CGCGGCTCAT GTCCTCGTGT GTGGACACCC CGCAGCAACT GCAGTCCGTT 
CTCGAGTCAC TCATTGCCAA GAAGCAAAGC CTCTGTGAGG CGCTCCAAGC GTGGAACAGC 
AGGTTACAGG ACCTCTTCCA GCAGGAAAAA GGTAGGAAGA GGCCTTCGGT TCCTCCCAGT 
CCTGGAAGAC TGAGACAAGG AGAAGAGAGC AAGATAAGTG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAACGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGTCAGAGCT CCACGAGCTC CACCCATCTC CAGCTGCCCA CTCCTCCTGA GGCCCTGGCT 
GAGCAGTTAG TGGGAGGGCC TTCTGACCTG GACACAGCCA GTGGCTCTGA AGATGTATTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TATAATCCCA TTCCATTTCC TTTCGATCCA 
GATAAACATT ATTTAATGTA TGAACACGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGTG GAACAATGCT GAAGAAGGGC TTCCAACCAA TAGTGCTTTA 
GATAACAGAC CAAAAAGTAG CAGTCCTATT AGATTACCTG AAGTCAGTGG AGGACAGACA 
AACCGTACAG TAGAAGGAGA ACCTCAACCA ACCAAAAAAG CTTCAGGAAT GCTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCTC AGAAGAGAGA GACCTTACGT 
GGGGCCGACA GTGCATACTA CCAGGTTGGG CAGACTGGCA AGGAGGGGAT GGAGAGTCAA 
GGCCTTGAGC CTCAAGATGA AGTAGATGGA GGAGACACAC AGAAGAAACA ACTCACAAAT 
CCCCATGTGG AACTTCAATT TTCTGACGCT AACGCCAAGT TTTACTGTCG ACTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGGGAAGTG ATTCTGGGCA GCAGTGAGGA AGATTTCATT 
CGTTCCCTTT CTCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACAG AAGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCGCA CTACTTCAAT TATATCACCA ATGCTGTTCA ACAGAAGAGG 
CCCACTGCTT TGGCTAAAAT TCTTGGTGTG TACAGAATTG GTTACAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTGAAAAC TGACACTGGG 
AAAGAGAGCT GTGACGTGGT TCTGCTGGAT GAGAACCTCC TGAAGATGGT TCGAGACAAC 
CCTCTGTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA TAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTTTGC TGGTTGGTCG AGATGACACT 
AGCAATGAGC TGGTTGTTGG GATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTCGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACCGTG 
GTCTCTCCAG AGTTATACAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTC AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245141.1
CDS	270..6488
Translation	MPELISEVDHVISSSERTHEMATDDKSSPTLDSANDLPHSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWSSPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENILPNPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSSSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQLFQLPNEQTKTLMFFEGCAQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQSPSFHFLAEGRGEEGASQEQVSGSSLPQDPECPFDALSSDDSTLLESRTVPEKGELDNKSVPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGDEQPETSQQTVEQQDPKSQMRAFRDPLQDDTGMYVTEEVTSSEDKRKTYSLTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQSMNGNVQTKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQSKHLDPFVHSKDTSCTSGSKSGNKTESDEERGLIPSDVLWPTKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKSWTEKMQARLMSSCVDTPQQLQSVLESLIAKKQSLCEALQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQLVGGPSDLDTASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNNAEEGLPTNSALDNRPKSSSPIRLPEVSGGQTNRTVEGEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGMESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_006245079.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245079.2

ORF Insert Sequence:

ATGCCCGAAC TTATTTCGGA GGTGGACCAT GTCATCTCTT CCTCTGAGCG GACTCATGAA 
ATGGCCACAG ATGACAAGAG CTCCCCGACA TTGGACTCTG CTAATGACTT GCCTCACTCT 
CCTGCCAGTC CCTCTCACCT CACTCACTTT AAACCGTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCGTTTGTA AATCTTTTTC GTTTTAACAA AGAGAGAGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGTCCAGCCC TCAGATCCCT 
TCAAGGACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AGCAGCTTAA TGAGGAGCTT 
CACCGGCGCT CTTCAGTGTT AGAGAATATT TTGCCAAATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT CCAGCTTCGA 
AGCCTCAGCA CAGTGTTGAA ACGCCTCAAG GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AGTATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACCA CCTTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGGGCA 
TGCACATACT GTAGAAAAAT AGCCTTAAGT TACGCTCATT CCACAGACAG TAATTCCATT 
GGGGAGGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGT 
GAACCTCGGA CCCCTGTTGG GAGTAGGAAA GCCAGTCGTA ACATCTTCTT AGAGGATGAC 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAC TCCTCGAGTA GTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGAA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTGAGT 
CCCCAGGCTA ACCGAAACTA CATCAGGACA GAGACTACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCATAC CAGTGGGATG 
GAATTTCAGG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATTGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTCGATGG ACGTTGGTTG GATTGTGTCA GTCATCATGA CCAGCTTTTC 
AGGGACGAGT ATGCATTGTA TAGACCACTG CAGAGTACTG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG GGTAGAAGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGGGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCT TACTCCAGCA GCTGCTTCAA 
AATGAGTCAT TGTCATCATC TTGGAGGGAC ATCATTGTGT CACTAGTTTG TCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCCG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAAA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC TTGCATTGAC 
CCCATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTCGT TGAGAAAACA GTGTCTCGGA TTGCTCAAGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GAGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACCTGGGC 
ACTTGCCACA AGTTTTATAT GCAGCTCTTT CAGTTGCCTA ACGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTGC ACAGCACCTC GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCCTT TCTCATGGAT GAGTTTGCTA TGCCTCCAAC GTTAACACAA 
AGCCCTTCGT TCCATTTTCT GGCTGAGGGA AGAGGGGAAG AGGGAGCTTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC CCAGGATCCT GAGTGCCCCT TTGATGCCCT GTCTTCTGAT 
GATAGCACTT TGTTGGAATC AAGGACTGTG CCTGAGAAGG GTGAGCTGGA CAATAAAAGT 
GTCCCACAGG CTGTTGCCTC CTTGAAGCAT CAAGATTACA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCACTGGTA CCCGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAGA TGAACAGCCA GAGACATCAC AGCAAACGGT TGAGCAACAG 
GACCCCAAAA GCCAGATGAG AGCTTTTAGA GACCCTCTAC AGGATGACAC TGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTACTCCTT GACATTTAAA 
CAGGAATTAA AAGACGTCAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGGTGCTCA ACTCGAGATT ACTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAATAA AGAGGTGAAG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGAGCATG AATGGCAATG TTCAGACCAA GTCCATACAA 
GTCTTACCTT CACATGAGCT AGTGAGCACC AGGATCGCTG AACATCTGGG GGACAGCCAG 
AGCTTGGGTA GAATGCTAGC TGACTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATACA TCATGTACTT CAGGGAGCAA ATCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATTCCAAGTG ATGTCTTATG GCCAACAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGCG TGCTCTTCAG CAGTTCTTCT 
GCCCAGTCCA GCAATGCGCC CAGTGCTTGT GTCAGCCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CTAGCATGTT CTGTGATACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATCCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA TTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCGATGTCA TTTGCAAAAT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACTCGCAG AGCCAATGCT GAGCCCTGCG GTCACTCCAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAATCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TGTGTGTTCC ACTGCCAAAA ATATTCATTA AGCGCCAAGC CCCGCTGAAG 
GTGTCTCTTC TTCAGGACCT CAAGGACTTC TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAAA GACTTGCGTC CTTGAAAACA GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAGGATA TCTTTGCACA AAAGGAGATG GAGGAGGGTG AGTTTAAGAG CTGGACTGAG 
AAGATGCAGG CGCGGCTCAT GTCCTCGTGT GTGGACACCC CGCAGCAACT GCAGTCCGTT 
CTCGAGTCAC TCATTGCCAA GAAGCAAAGC CTCTGTGAGG CGCTCCAAGC GTGGAACAGC 
AGGTTACAGG ACCTCTTCCA GCAGGAAAAA GGTAGGAAGA GGCCTTCGGT TCCTCCCAGT 
CCTGGAAGAC TGAGACAAGG AGAAGAGAGC AAGATAAGTG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAACGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGTCAGAGCT CCACGAGCTC CACCCATCTC CAGCTGCCCA CTCCTCCTGA GGCCCTGGCT 
GAGCAGTTAG TGGGAGGGCC TTCTGACCTG GACACAGCCA GTGGCTCTGA AGATGTATTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TATAATCCCA TTCCATTTCC TTTCGATCCA 
GATAAACATT ATTTAATGTA TGAACACGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CAACTCTGTG GAACAATGCT GAAGAAGGGC TTCCAACCAA TAGTGCTTTA 
GATAACAGAC CAAAAAGTAG CAGTCCTATT AGATTACCTG AAGTCAGTGG AGGACAGACA 
AACCGTACAG TAGAAGGAGA ACCTCAACCA ACCAAAAAAG CTTCAGGAAT GCTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGCCCTGAT CTGTCTTCTC AGAAGAGAGA GACCTTACGT 
GGGGCCGACA GTGCATACTA CCAGGTTGGG CAGACTGGCA AGGAGGGGAT GGAGAGTCAA 
GGCCTTGAGC CTCAAGATGA AGTAGATGGA GGAGACACAC AGAAGAAACA ACTCACAAAT 
CCCCATGTGG AACTTCAATT TTCTGACGCT AACGCCAAGT TTTACTGTCG ACTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGGGAAGTG ATTCTGGGCA GCAGTGAGGA AGATTTCATT 
CGTTCCCTTT CTCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACAG AAGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCGCA CTACTTCAAT TATATCACCA ATGCTGTTCA ACAGAAGAGG 
CCCACTGCTT TGGCTAAAAT TCTTGGTGTG TACAGAATTG GTTACAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTGAAAAC TGACACTGGG 
AAAGAGAGCT GTGACGTGGT TCTGCTGGAT GAGAACCTCC TGAAGATGGT TCGAGACAAC 
CCTCTGTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA TAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTTTGC TGGTTGGTCG AGATGACACT 
AGCAATGAGC TGGTTGTTGG GATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTCGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACCGTG 
GTCTCTCCAG AGTTATACAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTC AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed