Bbc3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Bbc3
Entrez Gene ID	317673
Full Name	Bcl-2 binding component 3
Synonyms	Puma
General protein information	Preferred Names Bcl-2 binding component 3 Names bcl-2-binding component 3 p53 up-regulated modulator of apoptosis
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA Protein Name

NM_173837.2 NP_776209.1 bcl-2-binding component 3
Pathways from BioSystems

mRNA	Protein	Name
NM_173837.2	NP_776209.1	bcl-2-binding component 3

Homologies

Homo sapiens (human)	BBC3	NP_055232.1
Pan troglodytes (chimpanzee)	BBC3	XP_001168815.1
Rattus norvegicus (Norway rat)	Bbc3	NP_776209.1
Canis lupus familiaris (dog)	BBC3	XP_005616461.1
Mus musculus (house mouse)	Bbc3	NP_573497.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of a proapoptotic gene (BBC3) in islet injury mediated by cold preservation and rewarming.
Omori K, Kobayashi E, Komatsu H, Rawson J, Agrawal G, Parimi M, Oancea AR, Valiente L, Ferreri K, Al-Abdullah IH, Kandeel F, Takahashi M, Mullen Y
American journal of physiology. Endocrinology and metabolism310(11)E1016-26(2016 Jun)

Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine.
Cai D, Huang E, Luo B, Yang Y, Zhang F, Liu C, Lin Z, Xie WB, Wang H
Cell death & disease7e2161(2016 Mar)

The regulation of p53 up-regulated modulator of apoptosis by JNK/c-Jun pathway in ?-amyloid-induced neuron death.
Akhter R, Sanphui P, Das H, Saha P, Biswas SC
Journal of neurochemistry134(6)1091-103(2015 Sep)

PUMA-mediated mitochondrial apoptotic disruption by hypoxic postconditioning.
Li Y, Guo Q, Liu X, Wang C, Song D
Apoptosis : an international journal on programmed cell death20(8)1026-32(2015 Aug)

Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA.
Xie W, Zhang L, Jiao H, Guan L, Zha J, Li X, Wu M, Wang Z, Han J, You H
Autophagy11(9)1623-35(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrated the involvement of BBC3/Bbc3 in cold preservation/rewarming-mediated islet injury, possibly through modulating HMGB1- and oxidative stress-mediated injury to islets.
Title: Involvement of a proapoptotic gene (BBC3) in islet injury mediated by cold preservation and rewarming.

we identified a novel AP1-binding site on rat puma gene which is necessary for direct binding of c-Jun with Puma promoter
Title: The regulation of p53 up-regulated modulator of apoptosis by JNK/c-Jun pathway in ?-amyloid-induced neuron death.

Reduction of p53 upregulated modulator of apoptosis (PUMA) expression mediates the endogenous cardioprotective mechanisms of postconditioning by disrupting mitochondrial apoptotic pathway.
Title: PUMA-mediated mitochondrial apoptotic disruption by hypoxic postconditioning.

Puma is activated by both p53 and PI3K/Akt/FoxO3a pathways and cooperates with Bim to induce neuron death in response to Abeta(1-42)
Title: The essential role of p53-up-regulated modulator of apoptosis (Puma) and its regulation by FoxO3a transcription factor in ?-amyloid-induced neuron death.

over-expression of PUMA correlates with cauda equina compression caused apoptosis of spinal cord cells
Title: Over-expression of PUMA correlates with the apoptosis of spinal cord cells in rat neuropathic intermittent claudication model.

The following Bbc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bbc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42677	NM_173837.2 Latest version!	Rattus norvegicus Bcl-2 binding component 3 (Bbc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bbc3 ( NM_173837.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_776209.1 Rattus norvegicus Bcl-2 binding component 3 (Bbc3), mRNA.

Price & Availability↑

CloneID	ORa42677
Clone ID Related Accession (Same CDS sequence)	NM_173837.2
Accession Version	NM_173837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	bcl-2-binding component 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY157758.1. On Jun 3, 2003 this sequence version replaced NM_173837.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY157758.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCCGCG CACGCCAGGA GGGCAGCTCT CCGGAGCCCG TAGAGGGCCT AGCCCGCGAC 
AGCCCGCGTC CTTTCCCGCT CGGCCGCCTG ATGCCCTCCG CTGTATCCTG CGGCCTCTGC 
GAGCCCGGCC TGCCCGCTGC CCCTGCTGCC CCTGCCTTGC TGCCGGCCGC CTACCTCTGC 
GCCCCCACCG CCCCGCCTGC CGTCACCGCC GCCCTGGGGG GCCCCCGCTG GCCTGGGGGT 
CACCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT GTCACCAGCC 
CAGCAGCACC TAGAGTCGCC CGTGCCCAGC GCCCCGGAGG CCCTGGCGGG AGGCCCCACC 
CAAGCTGCCC CGGGAGTGCG TGTGGAGGAG GAGGAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGAGGA TGGCGGACGA CCTCAACGCG CAGTACGAGC GGCGGAGACA AGAAGAGCAA 
CATCGACACC GACCCTCGCC CTGGAGGGTC ATGTATAATC TCTTCATGGG ACTCCTCCCC 
TTACCCAGGG ATCCTGGAGC CCCAGAAATG GAGCCCAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776209.1
CDS	279..860
Translation	MARARQEGSSPEPVEGLARDSPRPFPLGRLMPSAVSCGLCEPGLPAAPAAPALLPAAYLCAPTAPPAVTAALGGPRWPGGHRSRPRGPRPDGPQPSLSPAQQHLESPVPSAPEALAGGPTQAAPGVRVEEEEWAREIGAQLRRMADDLNAQYERRRQEEQHRHRPSPWRVMYNLFMGLLPLPRDPGAPEMEPN

Target ORF information:

RefSeq Version	NM_173837.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Bcl-2 binding component 3 (Bbc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173837.2

ORF Insert Sequence:

ATGGCCCGCG CACGCCAGGA GGGCAGCTCT CCGGAGCCCG TAGAGGGCCT AGCCCGCGAC 
AGCCCGCGTC CTTTCCCGCT CGGCCGCCTG ATGCCCTCCG CTGTATCCTG CGGCCTCTGC 
GAGCCCGGCC TGCCCGCTGC CCCTGCTGCC CCTGCCTTGC TGCCGGCCGC CTACCTCTGC 
GCCCCCACCG CCCCGCCTGC CGTCACCGCC GCCCTGGGGG GCCCCCGCTG GCCTGGGGGT 
CACCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT GTCACCAGCC 
CAGCAGCACC TAGAGTCGCC CGTGCCCAGC GCCCCGGAGG CCCTGGCGGG AGGCCCCACC 
CAAGCTGCCC CGGGAGTGCG TGTGGAGGAG GAGGAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGAGGA TGGCGGACGA CCTCAACGCG CAGTACGAGC GGCGGAGACA AGAAGAGCAA 
CATCGACACC GACCCTCGCC CTGGAGGGTC ATGTATAATC TCTTCATGGG ACTCCTCCCC 
TTACCCAGGG ATCCTGGAGC CCCAGAAATG GAGCCCAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of a proapoptotic gene (BBC3) in islet injury mediated by cold preservation and rewarming.
American journal of physiology. Endocrinology and metabolism310(11)E1016-26(2016 Jun)
Omori K,Kobayashi E,Komatsu H,Rawson J,Agrawal G,Parimi M,Oancea AR,Valiente L,Ferreri K,Al-Abdullah IH,Kandeel F,Takahashi M,Mullen Y

Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine.
Cell death & disease7e2161(2016 Mar)
Cai D,Huang E,Luo B,Yang Y,Zhang F,Liu C,Lin Z,Xie WB,Wang H

The regulation of p53 up-regulated modulator of apoptosis by JNK/c-Jun pathway in ?-amyloid-induced neuron death.
Journal of neurochemistry134(6)1091-103(2015 Sep)
Akhter R,Sanphui P,Das H,Saha P,Biswas SC

PUMA-mediated mitochondrial apoptotic disruption by hypoxic postconditioning.
Apoptosis : an international journal on programmed cell death20(8)1026-32(2015 Aug)
Li Y,Guo Q,Liu X,Wang C,Song D

Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA.
Autophagy11(9)1623-35(2015)
Xie W,Zhang L,Jiao H,Guan L,Zha J,Li X,Wu M,Wang Z,Han J,You H

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Bbc3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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