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Gas8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Gas8
Entrez Gene ID 361438
Full Name growth arrest specific 8
General protein information
Preferred Names

growth arrest specific 8

Names

growth arrest-specific protein 8
GAS-11
GAS-8
growth arrest-specific protein 11
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

19

19q12

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006255779.3 XP_006255841.1 growth arrest-specific protein 8 isoform X3
XM_008772637.2 XP_008770859.1 growth arrest-specific protein 8 isoform X6
XM_017601329.1 XP_017456818.1 growth arrest-specific protein 8 isoform X2
XM_008772635.2 XP_008770857.1 growth arrest-specific protein 8 isoform X4
XM_006255777.3 XP_006255839.1 growth arrest-specific protein 8 isoform X1
XM_008772636.2 XP_008770858.1 growth arrest-specific protein 8 isoform X5
NM_001039030.1 NP_001034119.1 dynein regulatory complex subunit 4

Pathways from BioSystems


Homologies


Danio rerio (zebrafish) gas8 NP_955928.1
Drosophila melanogaster (fruit fly) Gas8 NP_570065.2
Homo sapiens (human) GAS8 NP_001472.1
Canis lupus familiaris (dog) GAS8 XP_005620585.1
Mus musculus (house mouse) Gas8 NP_061343.2
Gallus gallus (chicken) GAS8 NP_001264546.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005850 XP_001237728.2
Pan troglodytes (chimpanzee) GAS8 XP_511182.2
Xenopus tropicalis (tropical clawed frog) gas8 NP_001106441.1
GAS8 XP_001093935.1
Bos taurus (cattle) GAS8 NP_001091038.1
Rattus norvegicus (Norway rat) Gas8 NP_001034119.1

Gene Ontology


Bibliography

Related articles in PubMed

A mutation in
Abdelhamed Z, Vuong SM, Hill L, Shula C, Timms A, Beier D, Campbell K, Mangano FT, Stottmann RW, Goto J
Development (Cambridge, England)145(1)(2018 Jan)

Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory Complex Subunit, Cause Primary Ciliary Dyskinesia with Axonemal Disorganization.
Jeanson L, Thomas L, Copin B, Coste A, Sermet-Gaudelus I, Dastot-Le Moal F, Duquesnoy P, Montantin G, Collot N, Tissier S, Papon JF, Clement A, Louis B, Escudier E, Amselem S, Legendre M
Human mutation37(8)776-85(2016 08)

Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease.
Lewis WR, Malarkey EB, Tritschler D, Bower R, Pasek RC, Porath JD, Birket SE, Saunier S, Antignac C, Knowles MR, Leigh MW, Zariwala MA, Challa AK, Kesterson RA, Rowe SM, Drummond IA, Parant JM, Hildebrandt F, Porter ME, Yoder BK, Berbari NF
PLoS genetics12(7)e1006220(2016 07)

Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskinesia and Disrupt the Nexin-Dynein Regulatory Complex.
Olbrich H, Cremers C, Loges NT, Werner C, Nielsen KG, Marthin JK, Philipsen M, Wallmeier J, Pennekamp P, Menchen T, Edelbusch C, Dougherty GW, Schwartz O, Thiele H, Altm?ller J, Rommelmann F, Omran H
American journal of human genetics97(4)546-54(2015 Oct)

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
Evron T, Philipp M, Lu J, Meloni AR, Burkhalter M, Chen W, Caron MG
The Journal of biological chemistry286(31)27676-86(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gas8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gas8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa08405 NM_001039030.1
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa47920 XM_008772636.2
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
ORa39025 XM_006255777.3
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
ORa39027 XM_006255779.3
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
ORa47919 XM_008772635.2
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
ORa47921 XM_008772637.2
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
ORa59466 XM_017601329.1
Latest version!		 Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa08405
Clone ID Related Accession (Same CDS sequence) NM_001039030.1
Accession Version NM_001039030.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-18
Organism Rattus norvegicus(Norway rat)
Product dynein regulatory complex subunit 4
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099759.1. On Jan 12, 2006 this sequence version replaced XM_341716.2. ##Evidence-Data-START## Transcript exon combination :: BC099759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCACCCA AAAGGAGAGG GAAGAAAGGG AAAGCCAAAG GGAATGCAGT CGTTGATGGG 
GTCGCACCAG AGGACATGTC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACC GGGAGCGGGA GGAGCGCAAC TACTTTCAGC TGGAGCGGGA CAAAATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT TTACAAGCAG 
AAAGTGAAGC ACCTTCTGTA CGAACACCAG AACAATCTGG CAGAGGTGAA GACTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCGCCC AAGAGGGCGC TCTGCGCAAG 
GACATGCGCG TGCTGAAAGT GGAGCTGAAG GAGCAGGAGC TGGCTAACGA GGTGGTGATA 
AAGAACCTGC GTCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATCGAGGC CAAATATGAT AAAAAGATGA AGATGCTTCG AGACGAGCTT 
GACCTGCGGA GAAAGACTGA GATCCACGAG GTGGAGGAGA GGAAGAACGG GCAGATCAGT 
ACGCTGATGC AGCGTCATGA GGAGGCCTTC ACTGACATCA AAAACTACTA CAATGACATC 
ACTCTCAACA ACCTGGCCCT CATCAATTCC CTCAAGGAGC AGATGGAGGA TATGAGGAAG 
AAGGAGGAGT ACCTGGAGAG GGAGATGGCG GAGGTGTCTC TGCAGAACAA GCGGCTGGCT 
GAGCCGCTGC AGAGAGCCAA GGAGGAGATG AGCGAGATGC AGAAGAGACT GGGGAACCAT 
GAGAGGGACA AGCAGATCCT GGCCTGTACG AAGGCACGTT TGAAGGTCAC TGAGAAGGAG 
CTGAAGGACC TGAAGTGGGA GCATGAGATC CTGGAGCAGA GGTTCATCAA GGTGCAGCAG 
GAGAGGGAAG AGCTCTATCG GAAGTTCACT GCAGCCATCC AGGAGGTGCA GCAGAAGACA 
GGCTTCAAGA ACCTGGTGCT GGAGCGTAAG CTGCAGGCAC TGAATGCTGC TGTGGAGAAG 
AGAGAGGTGC AGTTCAATGA GGTGCTGGCT GCCTCCAACC TGGACCCTAC AGCACTAACA 
CTTGTGTCTC GCAAACTTGA GGACGTTCTA GAGTCGAAGA ATACCACCAT CAAGGACTTG 
CAGTATGAGC TGGCCCGAGT CTGCAAGGCC CACAACGACC TGTTGCGTAC ATATGAGGCA 
AAGTTATTGG CCTTCGGGAT CCCCCTGGAC AATGTGGGGT TCAAGCCCCT GGAGACAGCA 
GTGATTGGAC AGACACTGGG ACAGGGCCCT GCAGGACTTG TGGGTGCGCC GACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001034119.1
CDS24..1460
Translation

Target ORF information:

RefSeq Version NM_001039030.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001039030.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCACCCA AAAGGAGAGG GAAGAAAGGG AAAGCCAAAG GGAATGCAGT CGTTGATGGG 
GTCGCACCAG AGGACATGTC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACC GGGAGCGGGA GGAGCGCAAC TACTTTCAGC TGGAGCGGGA CAAAATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT TTACAAGCAG 
AAAGTGAAGC ACCTTCTGTA CGAACACCAG AACAATCTGG CAGAGGTGAA GACTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCGCCC AAGAGGGCGC TCTGCGCAAG 
GACATGCGCG TGCTGAAAGT GGAGCTGAAG GAGCAGGAGC TGGCTAACGA GGTGGTGATA 
AAGAACCTGC GTCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATCGAGGC CAAATATGAT AAAAAGATGA AGATGCTTCG AGACGAGCTT 
GACCTGCGGA GAAAGACTGA GATCCACGAG GTGGAGGAGA GGAAGAACGG GCAGATCAGT 
ACGCTGATGC AGCGTCATGA GGAGGCCTTC ACTGACATCA AAAACTACTA CAATGACATC 
ACTCTCAACA ACCTGGCCCT CATCAATTCC CTCAAGGAGC AGATGGAGGA TATGAGGAAG 
AAGGAGGAGT ACCTGGAGAG GGAGATGGCG GAGGTGTCTC TGCAGAACAA GCGGCTGGCT 
GAGCCGCTGC AGAGAGCCAA GGAGGAGATG AGCGAGATGC AGAAGAGACT GGGGAACCAT 
GAGAGGGACA AGCAGATCCT GGCCTGTACG AAGGCACGTT TGAAGGTCAC TGAGAAGGAG 
CTGAAGGACC TGAAGTGGGA GCATGAGATC CTGGAGCAGA GGTTCATCAA GGTGCAGCAG 
GAGAGGGAAG AGCTCTATCG GAAGTTCACT GCAGCCATCC AGGAGGTGCA GCAGAAGACA 
GGCTTCAAGA ACCTGGTGCT GGAGCGTAAG CTGCAGGCAC TGAATGCTGC TGTGGAGAAG 
AGAGAGGTGC AGTTCAATGA GGTGCTGGCT GCCTCCAACC TGGACCCTAC AGCACTAACA 
CTTGTGTCTC GCAAACTTGA GGACGTTCTA GAGTCGAAGA ATACCACCAT CAAGGACTTG 
CAGTATGAGC TGGCCCGAGT CTGCAAGGCC CACAACGACC TGTTGCGTAC ATATGAGGCA 
AAGTTATTGG CCTTCGGGAT CCCCCTGGAC AATGTGGGGT TCAAGCCCCT GGAGACAGCA 
GTGATTGGAC AGACACTGGG ACAGGGCCCT GCAGGACTTG TGGGTGCGCC GACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa47920
Clone ID Related Accession (Same CDS sequence) XM_008772636.2
Accession Version XM_008772636.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 918bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGAAATG ACTTTGAAAG GCAAGTTCGA GAAATCGAGG CCAAATATGA TAAAAAGATG 
AAGATGCTTC GAGACGAGCT TGACCTGCGG AGAAAGACTG AGATCCACGA GGTGGAGGAG 
AGGAAGAACG GGCAGATCAG TACGCTGATG CAGCGTCATG AGGAGGCCTT CACTGACATC 
AAAAACTACT ACAATGACAT CACTCTCAAC AACCTGGCCC TCATCAATTC CCTCAAGGAG 
CAGATGGAGG ATATGAGGAA GAAGGAGGAG TACCTGGAGA GGGAGATGGC GGAGGTGTCT 
CTGCAGAACA AGCGGCTGGC TGAGCCGCTG CAGAGAGCCA AGGAGGAGAT GAGCGAGATG 
CAGAAGAGAC TGGGGAACCA TGAGAGGGAC AAGCAGATCC TGGCCTGTAC GAAGGCACGT 
TTGAAGGTCA CTGAGAAGGA GCTGAAGGAC CTGAAGTGGG AGCATGAGAT CCTGGAGCAG 
AGGTTCATCA AGGTGCAGCA GGAGAGGGAA GAGCTCTATC GGAAGTTCAC TGCAGCCATC 
CAGGAGGTGC AGCAGAAGAC AGGCTTCAAG AACCTGGTGC TGGAGCGTAA GCTGCAGGCA 
CTGAATGCTG CTGTGGAGAA GAGAGAGGTG CAGTTCAATG AGGTGCTGGC TGCCTCCAAC 
CTGGACCCTA CAGCACTAAC ACTTGTGTCT CGCAAACTTG AGGACGTTCT AGAGTCGAAG 
AATACCACCA TCAAGGACTT GCAGTATGAG CTGGCCCGAG TCTGCAAGGC CCACAACGAC 
CTGTTGCGTA CATATGAGGC AAAGTTATTG GCCTTCGGGA TCCCCCTGGA CAATGTGGGG 
TTCAAGCCCC TGGAGACAGC AGTGATTGGA CAGACACTGG GACAGGGCCC TGCAGGACTT 
GTGGGTGCGC CGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008770858.1
CDS114..1031
Translation

Target ORF information:

RefSeq Version XM_008772636.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008772636.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGAAATG ACTTTGAAAG GCAAGTTCGA GAAATCGAGG CCAAATATGA TAAAAAGATG 
AAGATGCTTC GAGACGAGCT TGACCTGCGG AGAAAGACTG AGATCCACGA GGTGGAGGAG 
AGGAAGAACG GGCAGATCAG TACGCTGATG CAGCGTCATG AGGAGGCCTT CACTGACATC 
AAAAACTACT ACAATGACAT CACTCTCAAC AACCTGGCCC TCATCAATTC CCTCAAGGAG 
CAGATGGAGG ATATGAGGAA GAAGGAGGAG TACCTGGAGA GGGAGATGGC GGAGGTGTCT 
CTGCAGAACA AGCGGCTGGC TGAGCCGCTG CAGAGAGCCA AGGAGGAGAT GAGCGAGATG 
CAGAAGAGAC TGGGGAACCA TGAGAGGGAC AAGCAGATCC TGGCCTGTAC GAAGGCACGT 
TTGAAGGTCA CTGAGAAGGA GCTGAAGGAC CTGAAGTGGG AGCATGAGAT CCTGGAGCAG 
AGGTTCATCA AGGTGCAGCA GGAGAGGGAA GAGCTCTATC GGAAGTTCAC TGCAGCCATC 
CAGGAGGTGC AGCAGAAGAC AGGCTTCAAG AACCTGGTGC TGGAGCGTAA GCTGCAGGCA 
CTGAATGCTG CTGTGGAGAA GAGAGAGGTG CAGTTCAATG AGGTGCTGGC TGCCTCCAAC 
CTGGACCCTA CAGCACTAAC ACTTGTGTCT CGCAAACTTG AGGACGTTCT AGAGTCGAAG 
AATACCACCA TCAAGGACTT GCAGTATGAG CTGGCCCGAG TCTGCAAGGC CCACAACGAC 
CTGTTGCGTA CATATGAGGC AAAGTTATTG GCCTTCGGGA TCCCCCTGGA CAATGTGGGG 
TTCAAGCCCC TGGAGACAGC AGTGATTGGA CAGACACTGG GACAGGGCCC TGCAGGACTT 
GTGGGTGCGC CGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa39025
Clone ID Related Accession (Same CDS sequence) XM_006255777.3
Accession Version XM_006255777.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGTGG TGGAGGAACA TGTCGCCCGC ATCCGGGAGG AGCTGGACCG GGAGCGGGAG 
GAGCGCAACT ACTTTCAGCT GGAGCGGGAC AAAATCCACA CCTTCTGGGA GATCACGCGG 
AGGCAGCTGG AGGAGAAGAA GGCTGAGCTG CGCAACAAAG ACCGGGAGAT GGAGGAGGCC 
GAGGAGAGGC ACCAGGTGGA GATCAAGGTT TACAAGCAGA AAGTGAAGCA CCTTCTGTAC 
GAACACCAGA ACAATCTGGC AGAGGTGAAG ACTGAGGGCA CCGTGGTCAT GAAGCTGGCC 
CAGAAGGAGC ACCGCGCCCA AGAGGGCGCT CTGCGCAAGG ACATGCGCGT GCTGAAAGTG 
GAGCTGAAGG AGCAGGAGCT GGCTAACGAG GTGGTGATAA AGAACCTGCG TCTGAAACAA 
GCCGAGGAGA TTACCAAGAT GCGAAATGAC TTTGAAAGGC AAGTTCGAGA AATCGAGGCC 
AAATATGATA AAAAGATGAA GATGCTTCGA GACGAGCTTG ACCTGCGGAG AAAGACTGAG 
ATCCACGAGG TGGAGGAGAG GAAGAACGGG CAGATCAGTA CGCTGATGCA GCGTCATGAG 
GAGGCCTTCA CTGACATCAA AAACTACTAC AATGACATCA CTCTCAACAA CCTGGCCCTC 
ATCAATTCCC TCAAGGAGCA GATGGAGGAT ATGAGGAAGA AGGAGGAGTA CCTGGAGAGG 
GAGATGGCGG AGGTGTCTCT GCAGAACAAG CGGCTGGCTG AGCCGCTGCA GAGAGCCAAG 
GAGGAGATGA GCGAGATGCA GAAGAGACTG GGGAACCATG AGAGGGACAA GCAGATCCTG 
GCCTGTACGA AGGCACGTTT GAAGGTCACT GAGAAGGAGC TGAAGGACCT GAAGTGGGAG 
CATGAGATCC TGGAGCAGAG GTTCATCAAG GTGCAGCAGG AGAGGGAAGA GCTCTATCGG 
AAGTTCACTG CAGCCATCCA GGAGGTGCAG CAGAAGACAG GCTTCAAGAA CCTGGTGCTG 
GAGCGTAAGC TGCAGGCACT GAATGCTGCT GTGGAGAAGA GAGAGGTGCA GTTCAATGAG 
GTGCTGGCTG CCTCCAACCT GGACCCTACA GCACTAACAC TTGTGTCTCG CAAACTTGAG 
GACGTTCTAG AGTCGAAGAA TACCACCATC AAGGACTTGC AGTATGAGCT GGCCCGAGTC 
TGCAAGGCCC ACAACGACCT GTTGCGTACA TATGAGGCAA AGTTATTGGC CTTCGGGATC 
CCCCTGGACA ATGTGGGGTT CAAGCCCCTG GAGACAGCAG TGATTGGACA GACACTGGGA 
CAGGGCCCTG CAGGACTTGT GGGTGCGCCG ACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006255839.1
CDS173..1528
Translation

Target ORF information:

RefSeq Version XM_006255777.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006255777.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGTGG TGGAGGAACA TGTCGCCCGC ATCCGGGAGG AGCTGGACCG GGAGCGGGAG 
GAGCGCAACT ACTTTCAGCT GGAGCGGGAC AAAATCCACA CCTTCTGGGA GATCACGCGG 
AGGCAGCTGG AGGAGAAGAA GGCTGAGCTG CGCAACAAAG ACCGGGAGAT GGAGGAGGCC 
GAGGAGAGGC ACCAGGTGGA GATCAAGGTT TACAAGCAGA AAGTGAAGCA CCTTCTGTAC 
GAACACCAGA ACAATCTGGC AGAGGTGAAG ACTGAGGGCA CCGTGGTCAT GAAGCTGGCC 
CAGAAGGAGC ACCGCGCCCA AGAGGGCGCT CTGCGCAAGG ACATGCGCGT GCTGAAAGTG 
GAGCTGAAGG AGCAGGAGCT GGCTAACGAG GTGGTGATAA AGAACCTGCG TCTGAAACAA 
GCCGAGGAGA TTACCAAGAT GCGAAATGAC TTTGAAAGGC AAGTTCGAGA AATCGAGGCC 
AAATATGATA AAAAGATGAA GATGCTTCGA GACGAGCTTG ACCTGCGGAG AAAGACTGAG 
ATCCACGAGG TGGAGGAGAG GAAGAACGGG CAGATCAGTA CGCTGATGCA GCGTCATGAG 
GAGGCCTTCA CTGACATCAA AAACTACTAC AATGACATCA CTCTCAACAA CCTGGCCCTC 
ATCAATTCCC TCAAGGAGCA GATGGAGGAT ATGAGGAAGA AGGAGGAGTA CCTGGAGAGG 
GAGATGGCGG AGGTGTCTCT GCAGAACAAG CGGCTGGCTG AGCCGCTGCA GAGAGCCAAG 
GAGGAGATGA GCGAGATGCA GAAGAGACTG GGGAACCATG AGAGGGACAA GCAGATCCTG 
GCCTGTACGA AGGCACGTTT GAAGGTCACT GAGAAGGAGC TGAAGGACCT GAAGTGGGAG 
CATGAGATCC TGGAGCAGAG GTTCATCAAG GTGCAGCAGG AGAGGGAAGA GCTCTATCGG 
AAGTTCACTG CAGCCATCCA GGAGGTGCAG CAGAAGACAG GCTTCAAGAA CCTGGTGCTG 
GAGCGTAAGC TGCAGGCACT GAATGCTGCT GTGGAGAAGA GAGAGGTGCA GTTCAATGAG 
GTGCTGGCTG CCTCCAACCT GGACCCTACA GCACTAACAC TTGTGTCTCG CAAACTTGAG 
GACGTTCTAG AGTCGAAGAA TACCACCATC AAGGACTTGC AGTATGAGCT GGCCCGAGTC 
TGCAAGGCCC ACAACGACCT GTTGCGTACA TATGAGGCAA AGTTATTGGC CTTCGGGATC 
CCCCTGGACA ATGTGGGGTT CAAGCCCCTG GAGACAGCAG TGATTGGACA GACACTGGGA 
CAGGGCCCTG CAGGACTTGT GGGTGCGCCG ACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa39027
Clone ID Related Accession (Same CDS sequence) XM_006255779.3
Accession Version XM_006255779.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCACCCA AAAGGAGAGG GAAGAAAGGG AAAGCCAAAG GGAATGCAGT CGTTGATGGG 
GTCGCACCAG AGGACATGTC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACC GGGAGCGGGA GGAGCGCAAC TACTTTCAGC TGGAGCGGGA CAAAATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT TTACAAGCAG 
AAAGTGAAGC ACCTTCTGTA CGAACACCAG AACAATCTGG CAGAGGTGAA GACTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCGCCC AAGAGGGCGC TCTGCGCAAG 
GACATGCGCG TGCTGAAAGT GGAGCTGAAG GAGCAGGAGC TGGCTAACGA GGTGGTGATA 
AAGAACCTGC GTCTGAAACA AGCCGAGGAG ATTACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATCGAGGC CAAATATGAT AAAAAGATGA AGATGCTTCG AGACGAGCTT 
GACCTGCGGA GAAAGACTGA GATCCACGAG GTGGAGGAGA GGAAGAACGG GCAGATCAGT 
ACGCTGATGC AGCGTCATGA GGAGGCCTTC ACTGACATCA AAAACTACTA CAATGACATC 
ACTCTCAACA ACCTGGCCCT CATCAATTCC CTCAAGGAGC AGATGGAGGA TATGAGGAAG 
AAGGAGGAGT ACCTGGAGAG GGAGATGGCG GAGGTGTCTC TGCAGAACAA GCGGCTGGCT 
GAGCCGCTGC AGAGAGCCAA GGAGGAGATG AGCGAGATGC AGAAGAGACT GGGGAACCAT 
GAGAGGGACA AGCAGATCCT GGCCTGTACG AAGGCACGTT TGAAGGTCAC TGAGAAGGAG 
CTGAAGGACC TGAAGTGGGA GCATGAGATC CTGGAGCAGA GGTTCATCAA GCTTTTTGAA 
CAACGGCGTA CCCTTGAGCA GCATCCAAGA TCGAGCACAC GTGGTTTCAC CACCCGTCCA 
GCTACAGAGT TCTCCCAGCC TCTAACTCCA TGTCCAGTGA ATATGCATAG CTTCAGTGCC 
TTGGGTCCCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006255841.1
CDS101..1252
Translation

Target ORF information:

RefSeq Version XM_006255779.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006255779.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCACCCA AAAGGAGAGG GAAGAAAGGG AAAGCCAAAG GGAATGCAGT CGTTGATGGG 
GTCGCACCAG AGGACATGTC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACC GGGAGCGGGA GGAGCGCAAC TACTTTCAGC TGGAGCGGGA CAAAATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT TTACAAGCAG 
AAAGTGAAGC ACCTTCTGTA CGAACACCAG AACAATCTGG CAGAGGTGAA GACTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCGCCC AAGAGGGCGC TCTGCGCAAG 
GACATGCGCG TGCTGAAAGT GGAGCTGAAG GAGCAGGAGC TGGCTAACGA GGTGGTGATA 
AAGAACCTGC GTCTGAAACA AGCCGAGGAG ATTACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATCGAGGC CAAATATGAT AAAAAGATGA AGATGCTTCG AGACGAGCTT 
GACCTGCGGA GAAAGACTGA GATCCACGAG GTGGAGGAGA GGAAGAACGG GCAGATCAGT 
ACGCTGATGC AGCGTCATGA GGAGGCCTTC ACTGACATCA AAAACTACTA CAATGACATC 
ACTCTCAACA ACCTGGCCCT CATCAATTCC CTCAAGGAGC AGATGGAGGA TATGAGGAAG 
AAGGAGGAGT ACCTGGAGAG GGAGATGGCG GAGGTGTCTC TGCAGAACAA GCGGCTGGCT 
GAGCCGCTGC AGAGAGCCAA GGAGGAGATG AGCGAGATGC AGAAGAGACT GGGGAACCAT 
GAGAGGGACA AGCAGATCCT GGCCTGTACG AAGGCACGTT TGAAGGTCAC TGAGAAGGAG 
CTGAAGGACC TGAAGTGGGA GCATGAGATC CTGGAGCAGA GGTTCATCAA GCTTTTTGAA 
CAACGGCGTA CCCTTGAGCA GCATCCAAGA TCGAGCACAC GTGGTTTCAC CACCCGTCCA 
GCTACAGAGT TCTCCCAGCC TCTAACTCCA TGTCCAGTGA ATATGCATAG CTTCAGTGCC 
TTGGGTCCCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa47919
Clone ID Related Accession (Same CDS sequence) XM_008772635.2
Accession Version XM_008772635.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACTTTGA AAGGCAAGTT CGAGGTCAGT TTCGCAGACA TACACGGTGT AGGATGCTGT 
CAGGGCACCC TCCAATTGCT GCTTGAAATC GAGGCCAAAT ATGATAAAAA GATGAAGATG 
CTTCGAGACG AGCTTGACCT GCGGAGAAAG ACTGAGATCC ACGAGGTGGA GGAGAGGAAG 
AACGGGCAGA TCAGTACGCT GATGCAGCGT CATGAGGAGG CCTTCACTGA CATCAAAAAC 
TACTACAATG ACATCACTCT CAACAACCTG GCCCTCATCA ATTCCCTCAA GGAGCAGATG 
GAGGATATGA GGAAGAAGGA GGAGTACCTG GAGAGGGAGA TGGCGGAGGT GTCTCTGCAG 
AACAAGCGGC TGGCTGAGCC GCTGCAGAGA GCCAAGGAGG AGATGAGCGA GATGCAGAAG 
AGACTGGGGA ACCATGAGAG GGACAAGCAG ATCCTGGCCT GTACGAAGGC ACGTTTGAAG 
GTCACTGAGA AGGAGCTGAA GGACCTGAAG TGGGAGCATG AGATCCTGGA GCAGAGGTTC 
ATCAAGGTGC AGCAGGAGAG GGAAGAGCTC TATCGGAAGT TCACTGCAGC CATCCAGGAG 
GTGCAGCAGA AGACAGGCTT CAAGAACCTG GTGCTGGAGC GTAAGCTGCA GGCACTGAAT 
GCTGCTGTGG AGAAGAGAGA GGTGCAGTTC AATGAGGTGC TGGCTGCCTC CAACCTGGAC 
CCTACAGCAC TAACACTTGT GTCTCGCAAA CTTGAGGACG TTCTAGAGTC GAAGAATACC 
ACCATCAAGG ACTTGCAGTA TGAGCTGGCC CGAGTCTGCA AGGCCCACAA CGACCTGTTG 
CGTACATATG AGGCAAAGTT ATTGGCCTTC GGGATCCCCC TGGACAATGT GGGGTTCAAG 
CCCCTGGAGA CAGCAGTGAT TGGACAGACA CTGGGACAGG GCCCTGCAGG ACTTGTGGGT 
GCGCCGACAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008770857.1
CDS122..1093
Translation

Target ORF information:

RefSeq Version XM_008772635.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008772635.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACTTTGA AAGGCAAGTT CGAGGTCAGT TTCGCAGACA TACACGGTGT AGGATGCTGT 
CAGGGCACCC TCCAATTGCT GCTTGAAATC GAGGCCAAAT ATGATAAAAA GATGAAGATG 
CTTCGAGACG AGCTTGACCT GCGGAGAAAG ACTGAGATCC ACGAGGTGGA GGAGAGGAAG 
AACGGGCAGA TCAGTACGCT GATGCAGCGT CATGAGGAGG CCTTCACTGA CATCAAAAAC 
TACTACAATG ACATCACTCT CAACAACCTG GCCCTCATCA ATTCCCTCAA GGAGCAGATG 
GAGGATATGA GGAAGAAGGA GGAGTACCTG GAGAGGGAGA TGGCGGAGGT GTCTCTGCAG 
AACAAGCGGC TGGCTGAGCC GCTGCAGAGA GCCAAGGAGG AGATGAGCGA GATGCAGAAG 
AGACTGGGGA ACCATGAGAG GGACAAGCAG ATCCTGGCCT GTACGAAGGC ACGTTTGAAG 
GTCACTGAGA AGGAGCTGAA GGACCTGAAG TGGGAGCATG AGATCCTGGA GCAGAGGTTC 
ATCAAGGTGC AGCAGGAGAG GGAAGAGCTC TATCGGAAGT TCACTGCAGC CATCCAGGAG 
GTGCAGCAGA AGACAGGCTT CAAGAACCTG GTGCTGGAGC GTAAGCTGCA GGCACTGAAT 
GCTGCTGTGG AGAAGAGAGA GGTGCAGTTC AATGAGGTGC TGGCTGCCTC CAACCTGGAC 
CCTACAGCAC TAACACTTGT GTCTCGCAAA CTTGAGGACG TTCTAGAGTC GAAGAATACC 
ACCATCAAGG ACTTGCAGTA TGAGCTGGCC CGAGTCTGCA AGGCCCACAA CGACCTGTTG 
CGTACATATG AGGCAAAGTT ATTGGCCTTC GGGATCCCCC TGGACAATGT GGGGTTCAAG 
CCCCTGGAGA CAGCAGTGAT TGGACAGACA CTGGGACAGG GCCCTGCAGG ACTTGTGGGT 
GCGCCGACAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa47921
Clone ID Related Accession (Same CDS sequence) XM_008772637.2
Accession Version XM_008772637.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 900bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATCGA GGCCAAATAT GATAAAAAGA TGAAGATGCT TCGAGACGAG 
CTTGACCTGC GGAGAAAGAC TGAGATCCAC GAGGTGGAGG AGAGGAAGAA CGGGCAGATC 
AGTACGCTGA TGCAGCGTCA TGAGGAGGCC TTCACTGACA TCAAAAACTA CTACAATGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAT TCCCTCAAGG AGCAGATGGA GGATATGAGG 
AAGAAGGAGG AGTACCTGGA GAGGGAGATG GCGGAGGTGT CTCTGCAGAA CAAGCGGCTG 
GCTGAGCCGC TGCAGAGAGC CAAGGAGGAG ATGAGCGAGA TGCAGAAGAG ACTGGGGAAC 
CATGAGAGGG ACAAGCAGAT CCTGGCCTGT ACGAAGGCAC GTTTGAAGGT CACTGAGAAG 
GAGCTGAAGG ACCTGAAGTG GGAGCATGAG ATCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTC ACTGCAGCCA TCCAGGAGGT GCAGCAGAAG 
ACAGGCTTCA AGAACCTGGT GCTGGAGCGT AAGCTGCAGG CACTGAATGC TGCTGTGGAG 
AAGAGAGAGG TGCAGTTCAA TGAGGTGCTG GCTGCCTCCA ACCTGGACCC TACAGCACTA 
ACACTTGTGT CTCGCAAACT TGAGGACGTT CTAGAGTCGA AGAATACCAC CATCAAGGAC 
TTGCAGTATG AGCTGGCCCG AGTCTGCAAG GCCCACAACG ACCTGTTGCG TACATATGAG 
GCAAAGTTAT TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC GCCGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008770859.1
CDS122..1021
Translation

Target ORF information:

RefSeq Version XM_008772637.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008772637.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATCGA GGCCAAATAT GATAAAAAGA TGAAGATGCT TCGAGACGAG 
CTTGACCTGC GGAGAAAGAC TGAGATCCAC GAGGTGGAGG AGAGGAAGAA CGGGCAGATC 
AGTACGCTGA TGCAGCGTCA TGAGGAGGCC TTCACTGACA TCAAAAACTA CTACAATGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAT TCCCTCAAGG AGCAGATGGA GGATATGAGG 
AAGAAGGAGG AGTACCTGGA GAGGGAGATG GCGGAGGTGT CTCTGCAGAA CAAGCGGCTG 
GCTGAGCCGC TGCAGAGAGC CAAGGAGGAG ATGAGCGAGA TGCAGAAGAG ACTGGGGAAC 
CATGAGAGGG ACAAGCAGAT CCTGGCCTGT ACGAAGGCAC GTTTGAAGGT CACTGAGAAG 
GAGCTGAAGG ACCTGAAGTG GGAGCATGAG ATCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTC ACTGCAGCCA TCCAGGAGGT GCAGCAGAAG 
ACAGGCTTCA AGAACCTGGT GCTGGAGCGT AAGCTGCAGG CACTGAATGC TGCTGTGGAG 
AAGAGAGAGG TGCAGTTCAA TGAGGTGCTG GCTGCCTCCA ACCTGGACCC TACAGCACTA 
ACACTTGTGT CTCGCAAACT TGAGGACGTT CTAGAGTCGA AGAATACCAC CATCAAGGAC 
TTGCAGTATG AGCTGGCCCG AGTCTGCAAG GCCCACAACG ACCTGTTGCG TACATATGAG 
GCAAAGTTAT TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC GCCGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa59466
Clone ID Related Accession (Same CDS sequence) XM_017601329.1
Accession Version XM_017601329.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product growth arrest-specific protein 8 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGG CCGAGGAGAG GCACCAGGTG GAGATCAAGG TTTACAAGCA GAAAGTGAAG 
CACCTTCTGT ACGAACACCA GAACAATCTG GCAGAGGTGA AGACTGAGGG CACCGTGGTC 
ATGAAGCTGG CCCAGAAGGA GCACCGCGCC CAAGAGGGCG CTCTGCGCAA GGACATGCGC 
GTGCTGAAAG TGGAGCTGAA GGAGCAGGAG CTGGCTAACG AGGTGGTGAT AAAGAACCTG 
CGTCTGAAAC AAGCCGAGGA GATTACCAAG ATGCGAAATG ACTTTGAAAG GCAAGTTCGA 
GAAATCGAGG CCAAATATGA TAAAAAGATG AAGATGCTTC GAGACGAGCT TGACCTGCGG 
AGAAAGACTG AGATCCACGA GGTGGAGGAG AGGAAGAACG GGCAGATCAG TACGCTGATG 
CAGCGTCATG AGGAGGCCTT CACTGACATC AAAAACTACT ACAATGACAT CACTCTCAAC 
AACCTGGCCC TCATCAATTC CCTCAAGGAG CAGATGGAGG ATATGAGGAA GAAGGAGGAG 
TACCTGGAGA GGGAGATGGC GGAGGTGTCT CTGCAGAACA AGCGGCTGGC TGAGCCGCTG 
CAGAGAGCCA AGGAGGAGAT GAGCGAGATG CAGAAGAGAC TGGGGAACCA TGAGAGGGAC 
AAGCAGATCC TGGCCTGTAC GAAGGCACGT TTGAAGGTCA CTGAGAAGGA GCTGAAGGAC 
CTGAAGTGGG AGCATGAGAT CCTGGAGCAG AGGTTCATCA AGGTGCAGCA GGAGAGGGAA 
GAGCTCTATC GGAAGTTCAC TGCAGCCATC CAGGAGGTGC AGCAGAAGAC AGGCTTCAAG 
AACCTGGTGC TGGAGCGTAA GCTGCAGGCA CTGAATGCTG CTGTGGAGAA GAGAGAGGTG 
CAGTTCAATG AGGTGCTGGC TGCCTCCAAC CTGGACCCTA CAGCACTAAC ACTTGTGTCT 
CGCAAACTTG AGGACGTTCT AGAGTCGAAG AATACCACCA TCAAGGACTT GCAGTATGAG 
CTGGCCCGAG TCTGCAAGGC CCACAACGAC CTGTTGCGTA CATATGAGGC AAAGTTATTG 
GCCTTCGGGA TCCCCCTGGA CAATGTGGGG TTCAAGCCCC TGGAGACAGC AGTGATTGGA 
CAGACACTGG GACAGGGCCC TGCAGGACTT GTGGGTGCGC CGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017456818.1
CDS393..1580
Translation

Target ORF information:

RefSeq Version XM_017601329.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus growth arrest specific 8 (Gas8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017601329.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGG CCGAGGAGAG GCACCAGGTG GAGATCAAGG TTTACAAGCA GAAAGTGAAG 
CACCTTCTGT ACGAACACCA GAACAATCTG GCAGAGGTGA AGACTGAGGG CACCGTGGTC 
ATGAAGCTGG CCCAGAAGGA GCACCGCGCC CAAGAGGGCG CTCTGCGCAA GGACATGCGC 
GTGCTGAAAG TGGAGCTGAA GGAGCAGGAG CTGGCTAACG AGGTGGTGAT AAAGAACCTG 
CGTCTGAAAC AAGCCGAGGA GATTACCAAG ATGCGAAATG ACTTTGAAAG GCAAGTTCGA 
GAAATCGAGG CCAAATATGA TAAAAAGATG AAGATGCTTC GAGACGAGCT TGACCTGCGG 
AGAAAGACTG AGATCCACGA GGTGGAGGAG AGGAAGAACG GGCAGATCAG TACGCTGATG 
CAGCGTCATG AGGAGGCCTT CACTGACATC AAAAACTACT ACAATGACAT CACTCTCAAC 
AACCTGGCCC TCATCAATTC CCTCAAGGAG CAGATGGAGG ATATGAGGAA GAAGGAGGAG 
TACCTGGAGA GGGAGATGGC GGAGGTGTCT CTGCAGAACA AGCGGCTGGC TGAGCCGCTG 
CAGAGAGCCA AGGAGGAGAT GAGCGAGATG CAGAAGAGAC TGGGGAACCA TGAGAGGGAC 
AAGCAGATCC TGGCCTGTAC GAAGGCACGT TTGAAGGTCA CTGAGAAGGA GCTGAAGGAC 
CTGAAGTGGG AGCATGAGAT CCTGGAGCAG AGGTTCATCA AGGTGCAGCA GGAGAGGGAA 
GAGCTCTATC GGAAGTTCAC TGCAGCCATC CAGGAGGTGC AGCAGAAGAC AGGCTTCAAG 
AACCTGGTGC TGGAGCGTAA GCTGCAGGCA CTGAATGCTG CTGTGGAGAA GAGAGAGGTG 
CAGTTCAATG AGGTGCTGGC TGCCTCCAAC CTGGACCCTA CAGCACTAAC ACTTGTGTCT 
CGCAAACTTG AGGACGTTCT AGAGTCGAAG AATACCACCA TCAAGGACTT GCAGTATGAG 
CTGGCCCGAG TCTGCAAGGC CCACAACGAC CTGTTGCGTA CATATGAGGC AAAGTTATTG 
GCCTTCGGGA TCCCCCTGGA CAATGTGGGG TTCAAGCCCC TGGAGACAGC AGTGATTGGA 
CAGACACTGG GACAGGGCCC TGCAGGACTT GTGGGTGCGC CGACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A mutation in
Development (Cambridge, England)145(1)(2018 Jan)
Abdelhamed Z,Vuong SM,Hill L,Shula C,Timms A,Beier D,Campbell K,Mangano FT,Stottmann RW,Goto J


book

Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory Complex Subunit, Cause Primary Ciliary Dyskinesia with Axonemal Disorganization.
Human mutation37(8)776-85(2016 08)
Jeanson L,Thomas L,Copin B,Coste A,Sermet-Gaudelus I,Dastot-Le Moal F,Duquesnoy P,Montantin G,Collot N,Tissier S,Papon JF,Clement A,Louis B,Escudier E,Amselem S,Legendre M


book

Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease.
PLoS genetics12(7)e1006220(2016 07)
Lewis WR,Malarkey EB,Tritschler D,Bower R,Pasek RC,Porath JD,Birket SE,Saunier S,Antignac C,Knowles MR,Leigh MW,Zariwala MA,Challa AK,Kesterson RA,Rowe SM,Drummond IA,Parant JM,Hildebrandt F,Porter ME,Yoder BK,Berbari NF


book

Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskinesia and Disrupt the Nexin-Dynein Regulatory Complex.
American journal of human genetics97(4)546-54(2015 Oct)
Olbrich H,Cremers C,Loges NT,Werner C,Nielsen KG,Marthin JK,Philipsen M,Wallmeier J,Pennekamp P,Menchen T,Edelbusch C,Dougherty GW,Schwartz O,Thiele H,Altm?ller J,Rommelmann F,Omran H


book

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
The Journal of biological chemistry286(31)27676-86(2011 Aug)
Evron T,Philipp M,Lu J,Meloni AR,Burkhalter M,Chen W,Caron MG