Hace1 cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following Hace1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hace1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa00363 NM_001108539.3
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $531.30
$759.00
ORa39553 XM_006256595.3
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
ORa39555 XM_006256597.3
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
ORa39556 XM_006256598.2
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa60132 XM_017601694.1
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
ORa60133 XM_017601695.1
Latest version!
Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa00363
    Clone ID Related Accession (Same CDS sequence) NM_001108539.3
    Accession Version NM_001108539.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-23
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07045530.1, AABR07045531.1, AABR07045532.1 and AABR07045534.1. On Nov 27, 2008 this sequence version replaced NM_001108539.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTCTGTCC AGCAGCTACG AGGCGCAGAT GAAGAGCCTC
    CTGCGGATCG TGAGGATCTT CTGCCACGTC TTCCGCATTG GCCCCTCGTC TCCCAGTAAC
    GGCATGGATA TGGGCTACAA TGGGAATAAG ACTCCAAGGA GCCAGGTGTT CAAGCCTTTG
    GAATTGCTTT GGCACTCTCT GGATGAGTGG TTGGTTTTAA TAGCCACAGA ATTAATGAAG
    AACAAAGAGG ACTCAGCGGA TATCACCTCC ATTTTACTGA AACAGAAAGG CCAGGATCAA
    GAGGCCTCTT GTATTTCAGC GTTTGAGCCA CCAGGACCTG GGAGCTATGA ACGCCTGTCC
    ACTGGTCCCG GGGAATCCAA ACCAGATGTG CTTGCCGGGA AGCAGGAAGC CAGTGCGGAC
    TGTCAGGATG TCATTTCTGT GACGGCCAAC CGGCTAAGTG CTGTCATTCA AGCCTTTTAC
    ATGTGCTGCT CCTGTCAGAT GCCTCCAGGA ATGACGTCGC CTCGGTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTGCT AAAGTGCTTT GTGAATAGAA ATCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA CTGATGTCGA GATTCATGCA CATCATAAAG
    GCACAGCCAT TCAAAGACCG CTGTGAATGG TTCTATGAGC ATTTGCACTC AGGACAGCCA
    GACTCAGACA TGGTGCACAG ACCAGTGAGT GAAAACGACA TTCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATCGTA TCAAAGGCCA ACTGTGCAAA GCTGAAGCAG
    GGGATCGCTG TGCGCTTTCA TGGAGAGGAA GGCATGGGTC AAGGTGTTGT ACGTGAATGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGACTATG CACTGTTTAC CCAGTCAGCA
    GATGGAACAA CTTTTCAGCC CAATAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAATC ACAGGCAGTT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAACACATT CTTGGTATTC CTGTGAATTA CCAAGATGTA
    GCGTCTATCG ATCCAGAATA TGCCAAAAAT CTGCAGTGGA TTTTAGACAA TGACATTAGT
    GACCTGGGTC TAGAACTGAC TTTCTCTGTT GAGACTGATG TGTTTGGAGC TATGGAAGAG
    GTACCGTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTGG TTACTGAACT CCGGATGACA AGAGCCATTC AGCCTCAGAT CAATGCGTTT
    TTACAGGGTT TCCATATGTT CATCCCTCCT TCCCTCATAC AGCTGTTCGA CGAGTATGAA
    TTGGAGCTAC TGCTCTCTGG CATGCCGGAA ATTGACGTGA ATGATTGGAT CAAAAATACA
    GAATACACAA GTGGCTATGA AAGGGAAGAT CCAGTTATTC AGTGGTTCTG GGAGGTTGTG
    GAGGACATGA CTCAAGAAGA GCGGGTCCTT CTCTTACAGT TCGTGACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATCATG GGTGGCAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACGTGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAGATC CTCAAGGACA GACTCCTCGT AGCTCTGCAT
    TGTGGCAGCT ACGGCTATAC AATGGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001102009.2
    CDS1..2730
    Translation

    Target ORF information:

    RefSeq Version NM_001108539.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001108539.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTCTGTCC AGCAGCTACG AGGCGCAGAT GAAGAGCCTC
    CTGCGGATCG TGAGGATCTT CTGCCACGTC TTCCGCATTG GCCCCTCGTC TCCCAGTAAC
    GGCATGGATA TGGGCTACAA TGGGAATAAG ACTCCAAGGA GCCAGGTGTT CAAGCCTTTG
    GAATTGCTTT GGCACTCTCT GGATGAGTGG TTGGTTTTAA TAGCCACAGA ATTAATGAAG
    AACAAAGAGG ACTCAGCGGA TATCACCTCC ATTTTACTGA AACAGAAAGG CCAGGATCAA
    GAGGCCTCTT GTATTTCAGC GTTTGAGCCA CCAGGACCTG GGAGCTATGA ACGCCTGTCC
    ACTGGTCCCG GGGAATCCAA ACCAGATGTG CTTGCCGGGA AGCAGGAAGC CAGTGCGGAC
    TGTCAGGATG TCATTTCTGT GACGGCCAAC CGGCTAAGTG CTGTCATTCA AGCCTTTTAC
    ATGTGCTGCT CCTGTCAGAT GCCTCCAGGA ATGACGTCGC CTCGGTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTGCT AAAGTGCTTT GTGAATAGAA ATCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA CTGATGTCGA GATTCATGCA CATCATAAAG
    GCACAGCCAT TCAAAGACCG CTGTGAATGG TTCTATGAGC ATTTGCACTC AGGACAGCCA
    GACTCAGACA TGGTGCACAG ACCAGTGAGT GAAAACGACA TTCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATCGTA TCAAAGGCCA ACTGTGCAAA GCTGAAGCAG
    GGGATCGCTG TGCGCTTTCA TGGAGAGGAA GGCATGGGTC AAGGTGTTGT ACGTGAATGG
    TTTGATATTC TGTCTAATGA GATAGTCAAT CCTGACTATG CACTGTTTAC CCAGTCAGCA
    GATGGAACAA CTTTTCAGCC CAATAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAATC ACAGGCAGTT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAACACATT CTTGGTATTC CTGTGAATTA CCAAGATGTA
    GCGTCTATCG ATCCAGAATA TGCCAAAAAT CTGCAGTGGA TTTTAGACAA TGACATTAGT
    GACCTGGGTC TAGAACTGAC TTTCTCTGTT GAGACTGATG TGTTTGGAGC TATGGAAGAG
    GTACCGTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAC
    GTCCAGCTGG TTACTGAACT CCGGATGACA AGAGCCATTC AGCCTCAGAT CAATGCGTTT
    TTACAGGGTT TCCATATGTT CATCCCTCCT TCCCTCATAC AGCTGTTCGA CGAGTATGAA
    TTGGAGCTAC TGCTCTCTGG CATGCCGGAA ATTGACGTGA ATGATTGGAT CAAAAATACA
    GAATACACAA GTGGCTATGA AAGGGAAGAT CCAGTTATTC AGTGGTTCTG GGAGGTTGTG
    GAGGACATGA CTCAAGAAGA GCGGGTCCTT CTCTTACAGT TCGTGACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATCATG GGTGGCAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACGTGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAGATC CTCAAGGACA GACTCCTCGT AGCTCTGCAT
    TGTGGCAGCT ACGGCTATAC AATGGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa39553
    Clone ID Related Accession (Same CDS sequence) XM_006256595.3
    Accession Version XM_006256595.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC
    GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT
    GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG
    AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA
    ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG
    AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT
    GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG
    AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT
    GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG
    CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA
    AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG
    AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG
    CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC
    ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC
    AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT
    CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT
    GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT
    CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT
    CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG
    ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT
    GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA CGTCCAGCTG GTTACTGAAC TCCGGATGAC AAGAGCCATT
    CAGCCTCAGA TCAATGCGTT TTTACAGGGT TTCCATATGT TCATCCCTCC TTCCCTCATA
    CAGCTGTTCG ACGAGTATGA ATTGGAGCTA CTGCTCTCTG GCATGCCGGA AATTGACGTG
    AATGATTGGA TCAAAAATAC AGAATACACA AGTGGCTATG AAAGGGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAGGTTGT GGAGGACATG ACTCAAGAAG AGCGGGTCCT TCTCTTACAG
    TTCGTGACTG GCAGTTCCAG GGTCCCGCAT GGTGGCTTTG CCAATATCAT GGGTGGCAGT
    GGTTTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTTTT ACCAACTTCC
    AGCACGTGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAGAT CCTCAAGGAC
    AGACTCCTCG TAGCTCTGCA TTGTGGCAGC TACGGCTATA CAATGGCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006256657.1
    CDS257..3007
    Translation

    Target ORF information:

    RefSeq Version XM_006256595.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006256595.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC
    GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT
    GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG
    AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA
    ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG
    AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT
    GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG
    AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT
    GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG
    CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA
    AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG
    AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG
    CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC
    ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC
    AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT
    CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT
    GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT
    CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT
    CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG
    ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT
    GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA CGTCCAGCTG GTTACTGAAC TCCGGATGAC AAGAGCCATT
    CAGCCTCAGA TCAATGCGTT TTTACAGGGT TTCCATATGT TCATCCCTCC TTCCCTCATA
    CAGCTGTTCG ACGAGTATGA ATTGGAGCTA CTGCTCTCTG GCATGCCGGA AATTGACGTG
    AATGATTGGA TCAAAAATAC AGAATACACA AGTGGCTATG AAAGGGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAGGTTGT GGAGGACATG ACTCAAGAAG AGCGGGTCCT TCTCTTACAG
    TTCGTGACTG GCAGTTCCAG GGTCCCGCAT GGTGGCTTTG CCAATATCAT GGGTGGCAGT
    GGTTTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTTTT ACCAACTTCC
    AGCACGTGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAGAT CCTCAAGGAC
    AGACTCCTCG TAGCTCTGCA TTGTGGCAGC TACGGCTATA CAATGGCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa39555
    Clone ID Related Accession (Same CDS sequence) XM_006256597.3
    Accession Version XM_006256597.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC
    GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT
    GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG
    AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA
    ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG
    AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT
    GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG
    AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT
    GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG
    CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA
    AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG
    AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG
    CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC
    ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC
    AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT
    CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT
    GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT
    CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT
    CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG
    ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT
    GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGAGCTACT GCTCTCTGGC ATGCCGGAAA TTGACGTGAA TGATTGGATC
    AAAAATACAG AATACACAAG TGGCTATGAA AGGGAAGATC CAGTTATTCA GTGGTTCTGG
    GAGGTTGTGG AGGACATGAC TCAAGAAGAG CGGGTCCTTC TCTTACAGTT CGTGACTGGC
    AGTTCCAGGG TCCCGCATGG TGGCTTTGCC AATATCATGG GTGGCAGTGG TTTGCAAAAC
    TTTACAATTG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCCAG CACGTGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAGATCC TCAAGGACAG ACTCCTCGTA
    GCTCTGCATT GTGGCAGCTA CGGCTATACA ATGGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006256659.1
    CDS257..2875
    Translation

    Target ORF information:

    RefSeq Version XM_006256597.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006256597.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA CGAGACTGCT GTTTACACAT TAATGCCAAT GGTGATGGCG
    GATCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTTGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCAGCAA ACTGTGGATC TGTGGAGTGC
    TTGGTTCTTC TGTTAAAGAA AGGAGCAAAC CCCAACTACC AAGACATTTC AGGCTGTACG
    CCCCTTCATC TGGCCGCTAG GAACGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCAGACG TCAACATTTG CAATAATGAA GGCCTTACCG CAATCCACTG GCTGGCTGTG
    AATGGAAGGA CAGAGCTACT CCATGACCTG GTGCAGCACG TCACGGATGT GGATGTGGAG
    GATGCAATGG GGCAGACAGC ATTACATGTG GCCTGCCAGA ACGGGCACAA AACGACCGTG
    CAGTGTCTGC TGGACAGCGG TGCTGACATC AACCGGCCGA ATGTGGCAGG AGCTACGCCA
    CTGTACTTTG CTTGCAGTCA TGGCCAGAGA GATACTGCGC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAACGGAGTA ACCCCTCTGG ACCTGTGTGT GCAGGGTGGC
    TATGGACAGA CTTGCGAAGT GTTAATTCAG TATCACCCCA GGCTTTTCCA GACGATTGTT
    CAAATGACGC AGAATGAAGA CCTTCGGGAG AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAGA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GTCTTGCTGA AGTTGCTACA
    ACAAATGGCC ATAAACTGCT TAGTGTGTTC CGGTTGGTCT CCAGTCTGTC CAGCAGCTAC
    GAGGCGCAGA TGAAGAGCCT CCTGCGGATC GTGAGGATCT TCTGCCACGT CTTCCGCATT
    GGCCCCTCGT CTCCCAGTAA CGGCATGGAT ATGGGCTACA ATGGGAATAA GACTCCAAGG
    AGCCAGGTGT TCAAGCCTTT GGAATTGCTT TGGCACTCTC TGGATGAGTG GTTGGTTTTA
    ATAGCCACAG AATTAATGAA GAACAAAGAG GACTCAGCGG ATATCACCTC CATTTTACTG
    AAACAGAAAG GCCAGGATCA AGAGGCCTCT TGTATTTCAG CGTTTGAGCC ACCAGGACCT
    GGGAGCTATG AACGCCTGTC CACTGGTCCC GGGGAATCCA AACCAGATGT GCTTGCCGGG
    AAGCAGGAAG CCAGTGCGGA CTGTCAGGAT GTCATTTCTG TGACGGCCAA CCGGCTAAGT
    GCTGTCATTC AAGCCTTTTA CATGTGCTGC TCCTGTCAGA TGCCTCCAGG AATGACGTCG
    CCTCGGTTCA TTGAGTTTGT CTGCAAGCAT GATGAAGTGC TAAAGTGCTT TGTGAATAGA
    AATCCCAAAA TTATATTTGA CCATTTTCAC TTTCTTCTTG AATGTCCTGA ACTGATGTCG
    AGATTCATGC ACATCATAAA GGCACAGCCA TTCAAAGACC GCTGTGAATG GTTCTATGAG
    CATTTGCACT CAGGACAGCC AGACTCAGAC ATGGTGCACA GACCAGTGAG TGAAAACGAC
    ATTCTCCTGG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAATCGT ATCAAAGGCC
    AACTGTGCAA AGCTGAAGCA GGGGATCGCT GTGCGCTTTC ATGGAGAGGA AGGCATGGGT
    CAAGGTGTTG TACGTGAATG GTTTGATATT CTGTCTAATG AGATAGTCAA TCCTGACTAT
    GCACTGTTTA CCCAGTCAGC AGATGGAACA ACTTTTCAGC CCAATAGCAA CTCCTATGTC
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAT
    CACAGGCAGT TGGTCAATAT TTACTTCACA CGATCCTTCT ACAAACACAT TCTTGGTATT
    CCTGTGAATT ACCAAGATGT AGCGTCTATC GATCCAGAAT ATGCCAAAAA TCTGCAGTGG
    ATTTTAGACA ATGACATTAG TGACCTGGGT CTAGAACTGA CTTTCTCTGT TGAGACTGAT
    GTGTTTGGAG CTATGGAAGA GGTACCGTTA AAGCCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGAGCTACT GCTCTCTGGC ATGCCGGAAA TTGACGTGAA TGATTGGATC
    AAAAATACAG AATACACAAG TGGCTATGAA AGGGAAGATC CAGTTATTCA GTGGTTCTGG
    GAGGTTGTGG AGGACATGAC TCAAGAAGAG CGGGTCCTTC TCTTACAGTT CGTGACTGGC
    AGTTCCAGGG TCCCGCATGG TGGCTTTGCC AATATCATGG GTGGCAGTGG TTTGCAAAAC
    TTTACAATTG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCCAG CACGTGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAGATCC TCAAGGACAG ACTCCTCGTA
    GCTCTGCATT GTGGCAGCTA CGGCTATACA ATGGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa39556
    Clone ID Related Accession (Same CDS sequence) XM_006256598.2
    Accession Version XM_006256598.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
    ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC
    CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC
    GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA
    ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT
    CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT
    GTGTTCCGGT TGGTCTCCAG TCTGTCCAGC AGCTACGAGG CGCAGATGAA GAGCCTCCTG
    CGGATCGTGA GGATCTTCTG CCACGTCTTC CGCATTGGCC CCTCGTCTCC CAGTAACGGC
    ATGGATATGG GCTACAATGG GAATAAGACT CCAAGGAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCTCTGGA TGAGTGGTTG GTTTTAATAG CCACAGAATT AATGAAGAAC
    AAAGAGGACT CAGCGGATAT CACCTCCATT TTACTGAAAC AGAAAGGCCA GGATCAAGAG
    GCCTCTTGTA TTTCAGCGTT TGAGCCACCA GGACCTGGGA GCTATGAACG CCTGTCCACT
    GGTCCCGGGG AATCCAAACC AGATGTGCTT GCCGGGAAGC AGGAAGCCAG TGCGGACTGT
    CAGGATGTCA TTTCTGTGAC GGCCAACCGG CTAAGTGCTG TCATTCAAGC CTTTTACATG
    TGCTGCTCCT GTCAGATGCC TCCAGGAATG ACGTCGCCTC GGTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTGCTAAA GTGCTTTGTG AATAGAAATC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAACTG ATGTCGAGAT TCATGCACAT CATAAAGGCA
    CAGCCATTCA AAGACCGCTG TGAATGGTTC TATGAGCATT TGCACTCAGG ACAGCCAGAC
    TCAGACATGG TGCACAGACC AGTGAGTGAA AACGACATTC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATCGTATCA AAGGCCAACT GTGCAAAGCT GAAGCAGGGG
    ATCGCTGTGC GCTTTCATGG AGAGGAAGGC ATGGGTCAAG GTGTTGTACG TGAATGGTTT
    GATATTCTGT CTAATGAGAT AGTCAATCCT GACTATGCAC TGTTTACCCA GTCAGCAGAT
    GGAACAACTT TTCAGCCCAA TAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGATTAGCT TTGAATCACA GGCAGTTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA ACACATTCTT GGTATTCCTG TGAATTACCA AGATGTAGCG
    TCTATCGATC CAGAATATGC CAAAAATCTG CAGTGGATTT TAGACAATGA CATTAGTGAC
    CTGGGTCTAG AACTGACTTT CTCTGTTGAG ACTGATGTGT TTGGAGCTAT GGAAGAGGTA
    CCGTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTACGTC
    CAGCTGGTTA CTGAACTCCG GATGACAAGA GCCATTCAGC CTCAGATCAA TGCGTTTTTA
    CAGGGTTTCC ATATGTTCAT CCCTCCTTCC CTCATACAGC TGTTCGACGA GTATGAATTG
    GAGCTACTGC TCTCTGGCAT GCCGGAAATT GACGTGAATG ATTGGATCAA AAATACAGAA
    TACACAAGTG GCTATGAAAG GGAAGATCCA GTTATTCAGT GGTTCTGGGA GGTTGTGGAG
    GACATGACTC AAGAAGAGCG GGTCCTTCTC TTACAGTTCG TGACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATCATGGGT GGCAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CGTGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAGATCCTC AAGGACAGAC TCCTCGTAGC TCTGCATTGT
    GGCAGCTACG GCTATACAAT GGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006256660.1
    CDS96..2342
    Translation

    Target ORF information:

    RefSeq Version XM_006256598.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006256598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
    ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC
    CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC
    GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA
    ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT
    CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT
    GTGTTCCGGT TGGTCTCCAG TCTGTCCAGC AGCTACGAGG CGCAGATGAA GAGCCTCCTG
    CGGATCGTGA GGATCTTCTG CCACGTCTTC CGCATTGGCC CCTCGTCTCC CAGTAACGGC
    ATGGATATGG GCTACAATGG GAATAAGACT CCAAGGAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCTCTGGA TGAGTGGTTG GTTTTAATAG CCACAGAATT AATGAAGAAC
    AAAGAGGACT CAGCGGATAT CACCTCCATT TTACTGAAAC AGAAAGGCCA GGATCAAGAG
    GCCTCTTGTA TTTCAGCGTT TGAGCCACCA GGACCTGGGA GCTATGAACG CCTGTCCACT
    GGTCCCGGGG AATCCAAACC AGATGTGCTT GCCGGGAAGC AGGAAGCCAG TGCGGACTGT
    CAGGATGTCA TTTCTGTGAC GGCCAACCGG CTAAGTGCTG TCATTCAAGC CTTTTACATG
    TGCTGCTCCT GTCAGATGCC TCCAGGAATG ACGTCGCCTC GGTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTGCTAAA GTGCTTTGTG AATAGAAATC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAACTG ATGTCGAGAT TCATGCACAT CATAAAGGCA
    CAGCCATTCA AAGACCGCTG TGAATGGTTC TATGAGCATT TGCACTCAGG ACAGCCAGAC
    TCAGACATGG TGCACAGACC AGTGAGTGAA AACGACATTC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATCGTATCA AAGGCCAACT GTGCAAAGCT GAAGCAGGGG
    ATCGCTGTGC GCTTTCATGG AGAGGAAGGC ATGGGTCAAG GTGTTGTACG TGAATGGTTT
    GATATTCTGT CTAATGAGAT AGTCAATCCT GACTATGCAC TGTTTACCCA GTCAGCAGAT
    GGAACAACTT TTCAGCCCAA TAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGATTAGCT TTGAATCACA GGCAGTTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA ACACATTCTT GGTATTCCTG TGAATTACCA AGATGTAGCG
    TCTATCGATC CAGAATATGC CAAAAATCTG CAGTGGATTT TAGACAATGA CATTAGTGAC
    CTGGGTCTAG AACTGACTTT CTCTGTTGAG ACTGATGTGT TTGGAGCTAT GGAAGAGGTA
    CCGTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTACGTC
    CAGCTGGTTA CTGAACTCCG GATGACAAGA GCCATTCAGC CTCAGATCAA TGCGTTTTTA
    CAGGGTTTCC ATATGTTCAT CCCTCCTTCC CTCATACAGC TGTTCGACGA GTATGAATTG
    GAGCTACTGC TCTCTGGCAT GCCGGAAATT GACGTGAATG ATTGGATCAA AAATACAGAA
    TACACAAGTG GCTATGAAAG GGAAGATCCA GTTATTCAGT GGTTCTGGGA GGTTGTGGAG
    GACATGACTC AAGAAGAGCG GGTCCTTCTC TTACAGTTCG TGACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATCATGGGT GGCAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CGTGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAGATCCTC AAGGACAGAC TCCTCGTAGC TCTGCATTGT
    GGCAGCTACG GCTATACAAT GGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60132
    Clone ID Related Accession (Same CDS sequence) XM_017601694.1
    Accession Version XM_017601694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTAAAT TACTGGAGTA CAGTGCAGAC GTCAACATTT GCAATAATGA AGGCCTTACC 
    GCAATCCACT GGCTGGCTGT GAATGGAAGG ACAGAGCTAC TCCATGACCT GGTGCAGCAC
    GTCACGGATG TGGATGTGGA GGATGCAATG GGGCAGACAG CATTACATGT GGCCTGCCAG
    AACGGGCACA AAACGACCGT GCAGTGTCTG CTGGACAGCG GTGCTGACAT CAACCGGCCG
    AATGTGGCAG GAGCTACGCC ACTGTACTTT GCTTGCAGTC ATGGCCAGAG AGATACTGCG
    CAGATTCTTC TATTACGAGG AGCCAAATAT TTACCAGATA AAAACGGAGT AACCCCTCTG
    GACCTGTGTG TGCAGGGTGG CTATGGACAG ACTTGCGAAG TGTTAATTCA GTATCACCCC
    AGGCTTTTCC AGACGATTGT TCAAATGACG CAGAATGAAG ACCTTCGGGA GAACATGTTA
    CGGCAAGTTC TGCAGCATTT GTCTCAGCAG AGTGAAAGCC AGTACCTAAA GATCCTGACA
    GGTCTTGCTG AAGTTGCTAC AACAAATGGC CATAAACTGC TTAGTGTGTT CCGGTTGGTC
    TCCAGTCTGT CCAGCAGCTA CGAGGCGCAG ATGAAGAGCC TCCTGCGGAT CGTGAGGATC
    TTCTGCCACG TCTTCCGCAT TGGCCCCTCG TCTCCCAGTA ACGGCATGGA TATGGGCTAC
    AATGGGAATA AGACTCCAAG GAGCCAGGTG TTCAAGCCTT TGGAATTGCT TTGGCACTCT
    CTGGATGAGT GGTTGGTTTT AATAGCCACA GAATTAATGA AGAACAAAGA GGACTCAGCG
    GATATCACCT CCATTTTACT GAAACAGAAA GGCCAGGATC AAGAGGCCTC TTGTATTTCA
    GCGTTTGAGC CACCAGGACC TGGGAGCTAT GAACGCCTGT CCACTGGTCC CGGGGAATCC
    AAACCAGATG TGCTTGCCGG GAAGCAGGAA GCCAGTGCGG ACTGTCAGGA TGTCATTTCT
    GTGACGGCCA ACCGGCTAAG TGCTGTCATT CAAGCCTTTT ACATGTGCTG CTCCTGTCAG
    ATGCCTCCAG GAATGACGTC GCCTCGGTTC ATTGAGTTTG TCTGCAAGCA TGATGAAGTG
    CTAAAGTGCT TTGTGAATAG AAATCCCAAA ATTATATTTG ACCATTTTCA CTTTCTTCTT
    GAATGTCCTG AACTGATGTC GAGATTCATG CACATCATAA AGGCACAGCC ATTCAAAGAC
    CGCTGTGAAT GGTTCTATGA GCATTTGCAC TCAGGACAGC CAGACTCAGA CATGGTGCAC
    AGACCAGTGA GTGAAAACGA CATTCTCCTG GTTCACAGAG ATTCTATTTT TAGGAGTAGC
    TGTGAAATCG TATCAAAGGC CAACTGTGCA AAGCTGAAGC AGGGGATCGC TGTGCGCTTT
    CATGGAGAGG AAGGCATGGG TCAAGGTGTT GTACGTGAAT GGTTTGATAT TCTGTCTAAT
    GAGATAGTCA ATCCTGACTA TGCACTGTTT ACCCAGTCAG CAGATGGAAC AACTTTTCAG
    CCCAATAGCA ACTCCTATGT CAATCCTGAT CACTTGAACT ATTTCCGGTT TGCTGGACAG
    ATCTTGGGAT TAGCTTTGAA TCACAGGCAG TTGGTCAATA TTTACTTCAC ACGATCCTTC
    TACAAACACA TTCTTGGTAT TCCTGTGAAT TACCAAGATG TAGCGTCTAT CGATCCAGAA
    TATGCCAAAA ATCTGCAGTG GATTTTAGAC AATGACATTA GTGACCTGGG TCTAGAACTG
    ACTTTCTCTG TTGAGACTGA TGTGTTTGGA GCTATGGAAG AGGTACCGTT AAAGCCTGGG
    GGTGGAAGTA TTCTTGTGAC ACAAAATAAC AAAGCGGAGT ACGTCCAGCT GGTTACTGAA
    CTCCGGATGA CAAGAGCCAT TCAGCCTCAG ATCAATGCGT TTTTACAGGG TTTCCATATG
    TTCATCCCTC CTTCCCTCAT ACAGCTGTTC GACGAGTATG AATTGGAGCT ACTGCTCTCT
    GGCATGCCGG AAATTGACGT GAATGATTGG ATCAAAAATA CAGAATACAC AAGTGGCTAT
    GAAAGGGAAG ATCCAGTTAT TCAGTGGTTC TGGGAGGTTG TGGAGGACAT GACTCAAGAA
    GAGCGGGTCC TTCTCTTACA GTTCGTGACT GGCAGTTCCA GGGTCCCGCA TGGTGGCTTT
    GCCAATATCA TGGGTGGCAG TGGTTTGCAA AACTTTACAA TTGCTGCTGT GCCATATACT
    CCAAATCTTT TACCAACTTC CAGCACGTGC ATCAACATGC TCAAGTTACC TGAATACCCA
    AGTAAAGAGA TCCTCAAGGA CAGACTCCTC GTAGCTCTGC ATTGTGGCAG CTACGGCTAT
    ACAATGGCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457183.1
    CDS1051..3462
    Translation

    Target ORF information:

    RefSeq Version XM_017601694.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTAAAT TACTGGAGTA CAGTGCAGAC GTCAACATTT GCAATAATGA AGGCCTTACC 
    GCAATCCACT GGCTGGCTGT GAATGGAAGG ACAGAGCTAC TCCATGACCT GGTGCAGCAC
    GTCACGGATG TGGATGTGGA GGATGCAATG GGGCAGACAG CATTACATGT GGCCTGCCAG
    AACGGGCACA AAACGACCGT GCAGTGTCTG CTGGACAGCG GTGCTGACAT CAACCGGCCG
    AATGTGGCAG GAGCTACGCC ACTGTACTTT GCTTGCAGTC ATGGCCAGAG AGATACTGCG
    CAGATTCTTC TATTACGAGG AGCCAAATAT TTACCAGATA AAAACGGAGT AACCCCTCTG
    GACCTGTGTG TGCAGGGTGG CTATGGACAG ACTTGCGAAG TGTTAATTCA GTATCACCCC
    AGGCTTTTCC AGACGATTGT TCAAATGACG CAGAATGAAG ACCTTCGGGA GAACATGTTA
    CGGCAAGTTC TGCAGCATTT GTCTCAGCAG AGTGAAAGCC AGTACCTAAA GATCCTGACA
    GGTCTTGCTG AAGTTGCTAC AACAAATGGC CATAAACTGC TTAGTGTGTT CCGGTTGGTC
    TCCAGTCTGT CCAGCAGCTA CGAGGCGCAG ATGAAGAGCC TCCTGCGGAT CGTGAGGATC
    TTCTGCCACG TCTTCCGCAT TGGCCCCTCG TCTCCCAGTA ACGGCATGGA TATGGGCTAC
    AATGGGAATA AGACTCCAAG GAGCCAGGTG TTCAAGCCTT TGGAATTGCT TTGGCACTCT
    CTGGATGAGT GGTTGGTTTT AATAGCCACA GAATTAATGA AGAACAAAGA GGACTCAGCG
    GATATCACCT CCATTTTACT GAAACAGAAA GGCCAGGATC AAGAGGCCTC TTGTATTTCA
    GCGTTTGAGC CACCAGGACC TGGGAGCTAT GAACGCCTGT CCACTGGTCC CGGGGAATCC
    AAACCAGATG TGCTTGCCGG GAAGCAGGAA GCCAGTGCGG ACTGTCAGGA TGTCATTTCT
    GTGACGGCCA ACCGGCTAAG TGCTGTCATT CAAGCCTTTT ACATGTGCTG CTCCTGTCAG
    ATGCCTCCAG GAATGACGTC GCCTCGGTTC ATTGAGTTTG TCTGCAAGCA TGATGAAGTG
    CTAAAGTGCT TTGTGAATAG AAATCCCAAA ATTATATTTG ACCATTTTCA CTTTCTTCTT
    GAATGTCCTG AACTGATGTC GAGATTCATG CACATCATAA AGGCACAGCC ATTCAAAGAC
    CGCTGTGAAT GGTTCTATGA GCATTTGCAC TCAGGACAGC CAGACTCAGA CATGGTGCAC
    AGACCAGTGA GTGAAAACGA CATTCTCCTG GTTCACAGAG ATTCTATTTT TAGGAGTAGC
    TGTGAAATCG TATCAAAGGC CAACTGTGCA AAGCTGAAGC AGGGGATCGC TGTGCGCTTT
    CATGGAGAGG AAGGCATGGG TCAAGGTGTT GTACGTGAAT GGTTTGATAT TCTGTCTAAT
    GAGATAGTCA ATCCTGACTA TGCACTGTTT ACCCAGTCAG CAGATGGAAC AACTTTTCAG
    CCCAATAGCA ACTCCTATGT CAATCCTGAT CACTTGAACT ATTTCCGGTT TGCTGGACAG
    ATCTTGGGAT TAGCTTTGAA TCACAGGCAG TTGGTCAATA TTTACTTCAC ACGATCCTTC
    TACAAACACA TTCTTGGTAT TCCTGTGAAT TACCAAGATG TAGCGTCTAT CGATCCAGAA
    TATGCCAAAA ATCTGCAGTG GATTTTAGAC AATGACATTA GTGACCTGGG TCTAGAACTG
    ACTTTCTCTG TTGAGACTGA TGTGTTTGGA GCTATGGAAG AGGTACCGTT AAAGCCTGGG
    GGTGGAAGTA TTCTTGTGAC ACAAAATAAC AAAGCGGAGT ACGTCCAGCT GGTTACTGAA
    CTCCGGATGA CAAGAGCCAT TCAGCCTCAG ATCAATGCGT TTTTACAGGG TTTCCATATG
    TTCATCCCTC CTTCCCTCAT ACAGCTGTTC GACGAGTATG AATTGGAGCT ACTGCTCTCT
    GGCATGCCGG AAATTGACGT GAATGATTGG ATCAAAAATA CAGAATACAC AAGTGGCTAT
    GAAAGGGAAG ATCCAGTTAT TCAGTGGTTC TGGGAGGTTG TGGAGGACAT GACTCAAGAA
    GAGCGGGTCC TTCTCTTACA GTTCGTGACT GGCAGTTCCA GGGTCCCGCA TGGTGGCTTT
    GCCAATATCA TGGGTGGCAG TGGTTTGCAA AACTTTACAA TTGCTGCTGT GCCATATACT
    CCAAATCTTT TACCAACTTC CAGCACGTGC ATCAACATGC TCAAGTTACC TGAATACCCA
    AGTAAAGAGA TCCTCAAGGA CAGACTCCTC GTAGCTCTGC ATTGTGGCAG CTACGGCTAT
    ACAATGGCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa60133
    Clone ID Related Accession (Same CDS sequence) XM_017601695.1
    Accession Version XM_017601695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HACE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
    ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC
    CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC
    GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA
    ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT
    CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT
    CTGTCCAGCA GCTACGAGGC GCAGATGAAG AGCCTCCTGC GGATCGTGAG GATCTTCTGC
    CACGTCTTCC GCATTGGCCC CTCGTCTCCC AGTAACGGCA TGGATATGGG CTACAATGGG
    AATAAGACTC CAAGGAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCTCTGGAT
    GAGTGGTTGG TTTTAATAGC CACAGAATTA ATGAAGAACA AAGAGGACTC AGCGGATATC
    ACCTCCATTT TACTGAAACA GAAAGGCCAG GATCAAGAGG CCTCTTGTAT TTCAGCGTTT
    GAGCCACCAG GACCTGGGAG CTATGAACGC CTGTCCACTG GTCCCGGGGA ATCCAAACCA
    GATGTGCTTG CCGGGAAGCA GGAAGCCAGT GCGGACTGTC AGGATGTCAT TTCTGTGACG
    GCCAACCGGC TAAGTGCTGT CATTCAAGCC TTTTACATGT GCTGCTCCTG TCAGATGCCT
    CCAGGAATGA CGTCGCCTCG GTTCATTGAG TTTGTCTGCA AGCATGATGA AGTGCTAAAG
    TGCTTTGTGA ATAGAAATCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAACTGA TGTCGAGATT CATGCACATC ATAAAGGCAC AGCCATTCAA AGACCGCTGT
    GAATGGTTCT ATGAGCATTT GCACTCAGGA CAGCCAGACT CAGACATGGT GCACAGACCA
    GTGAGTGAAA ACGACATTCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATCGTATCAA AGGCCAACTG TGCAAAGCTG AAGCAGGGGA TCGCTGTGCG CTTTCATGGA
    GAGGAAGGCA TGGGTCAAGG TGTTGTACGT GAATGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ACTATGCACT GTTTACCCAG TCAGCAGATG GAACAACTTT TCAGCCCAAT
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTT TGAATCACAG GCAGTTGGTC AATATTTACT TCACACGATC CTTCTACAAA
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTAGCGT CTATCGATCC AGAATATGCC
    AAAAATCTGC AGTGGATTTT AGACAATGAC ATTAGTGACC TGGGTCTAGA ACTGACTTTC
    TCTGTTGAGA CTGATGTGTT TGGAGCTATG GAAGAGGTAC CGTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTACGTCC AGCTGGTTAC TGAACTCCGG
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCGTTTTTAC AGGGTTTCCA TATGTTCATC
    CCTCCTTCCC TCATACAGCT GTTCGACGAG TATGAATTGG AGCTACTGCT CTCTGGCATG
    CCGGAAATTG ACGTGAATGA TTGGATCAAA AATACAGAAT ACACAAGTGG CTATGAAAGG
    GAAGATCCAG TTATTCAGTG GTTCTGGGAG GTTGTGGAGG ACATGACTCA AGAAGAGCGG
    GTCCTTCTCT TACAGTTCGT GACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATCATGGGTG GCAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC GTGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAGATCCTCA AGGACAGACT CCTCGTAGCT CTGCATTGTG GCAGCTACGG CTATACAATG
    GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017457184.1
    CDS96..2321
    Translation

    Target ORF information:

    RefSeq Version XM_017601695.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGGAC AGAGCTACTC CATGACCTGG TGCAGCACGT CACGGATGTG GATGTGGAGG 
    ATGCAATGGG GCAGACAGCA TTACATGTGG CCTGCCAGAA CGGGCACAAA ACGTCATGGC
    CAGAGAGATA CTGCGCAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAC
    GGAGTAACCC CTCTGGACCT GTGTGTGCAG GGTGGCTATG GACAGACTTG CGAAGTGTTA
    ATTCAGTATC ACCCCAGGCT TTTCCAGACG ATTGTTCAAA TGACGCAGAA TGAAGACCTT
    CGGGAGAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAGAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGTCT TGCTGAAGTT GCTACAACAA ATGGCCATAA ACTGCTTAGT
    CTGTCCAGCA GCTACGAGGC GCAGATGAAG AGCCTCCTGC GGATCGTGAG GATCTTCTGC
    CACGTCTTCC GCATTGGCCC CTCGTCTCCC AGTAACGGCA TGGATATGGG CTACAATGGG
    AATAAGACTC CAAGGAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCTCTGGAT
    GAGTGGTTGG TTTTAATAGC CACAGAATTA ATGAAGAACA AAGAGGACTC AGCGGATATC
    ACCTCCATTT TACTGAAACA GAAAGGCCAG GATCAAGAGG CCTCTTGTAT TTCAGCGTTT
    GAGCCACCAG GACCTGGGAG CTATGAACGC CTGTCCACTG GTCCCGGGGA ATCCAAACCA
    GATGTGCTTG CCGGGAAGCA GGAAGCCAGT GCGGACTGTC AGGATGTCAT TTCTGTGACG
    GCCAACCGGC TAAGTGCTGT CATTCAAGCC TTTTACATGT GCTGCTCCTG TCAGATGCCT
    CCAGGAATGA CGTCGCCTCG GTTCATTGAG TTTGTCTGCA AGCATGATGA AGTGCTAAAG
    TGCTTTGTGA ATAGAAATCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAACTGA TGTCGAGATT CATGCACATC ATAAAGGCAC AGCCATTCAA AGACCGCTGT
    GAATGGTTCT ATGAGCATTT GCACTCAGGA CAGCCAGACT CAGACATGGT GCACAGACCA
    GTGAGTGAAA ACGACATTCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATCGTATCAA AGGCCAACTG TGCAAAGCTG AAGCAGGGGA TCGCTGTGCG CTTTCATGGA
    GAGGAAGGCA TGGGTCAAGG TGTTGTACGT GAATGGTTTG ATATTCTGTC TAATGAGATA
    GTCAATCCTG ACTATGCACT GTTTACCCAG TCAGCAGATG GAACAACTTT TCAGCCCAAT
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTT TGAATCACAG GCAGTTGGTC AATATTTACT TCACACGATC CTTCTACAAA
    CACATTCTTG GTATTCCTGT GAATTACCAA GATGTAGCGT CTATCGATCC AGAATATGCC
    AAAAATCTGC AGTGGATTTT AGACAATGAC ATTAGTGACC TGGGTCTAGA ACTGACTTTC
    TCTGTTGAGA CTGATGTGTT TGGAGCTATG GAAGAGGTAC CGTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTACGTCC AGCTGGTTAC TGAACTCCGG
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCGTTTTTAC AGGGTTTCCA TATGTTCATC
    CCTCCTTCCC TCATACAGCT GTTCGACGAG TATGAATTGG AGCTACTGCT CTCTGGCATG
    CCGGAAATTG ACGTGAATGA TTGGATCAAA AATACAGAAT ACACAAGTGG CTATGAAAGG
    GAAGATCCAG TTATTCAGTG GTTCTGGGAG GTTGTGGAGG ACATGACTCA AGAAGAGCGG
    GTCCTTCTCT TACAGTTCGT GACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATCATGGGTG GCAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC GTGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAGATCCTCA AGGACAGACT CCTCGTAGCT CTGCATTGTG GCAGCTACGG CTATACAATG
    GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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