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Tor2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Tor2a
Entrez Gene ID 362112
Full Name torsin family 2, member A
General protein information
Preferred Names

torsin family 2, member A

Names

torsin-2A
prosalusin
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

3

3p11

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017591883.1 XP_017447372.1 torsin-2A isoform X2
NM_001007744.1 NP_001007745.1 torsin-2A precursor
XM_006233981.3 XP_006234043.1 torsin-2A isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) TOR2A NP_001078816.1
Canis lupus familiaris (dog) TOR2A XP_851500.3
Mus musculus (house mouse) Tor2a NP_690013.1
Danio rerio (zebrafish) tor2a NP_001029352.1
TOR2A XP_001095206.1
Bos taurus (cattle) TOR2A NP_001076945.1
Pan troglodytes (chimpanzee) TOR2A XP_520274.2
Gallus gallus (chicken) TOR2A XP_415507.4
Rattus norvegicus (Norway rat) Tor2a NP_001007745.1
Xenopus tropicalis (tropical clawed frog) tor2a NP_001090629.1

Gene Ontology


Bibliography

Related articles in PubMed

Silencing salusin-? attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.
Ren XS, Ling L, Zhou B, Han Y, Zhou YB, Chen Q, Li YH, Kang YM, Zhu GQ
Scientific reports743259(2017 Feb)

Salusin-? Promotes Vascular Smooth Muscle Cell Migration and Intimal Hyperplasia After Vascular Injury via ROS/NF?B/MMP-9 Pathway.
Sun HJ, Zhao MX, Ren XS, Liu TY, Chen Q, Li YH, Kang YM, Wang JJ, Zhu GQ
Antioxidants & redox signaling24(18)1045-57(2016 Jun)

Anti-salusin-? antibody enhances angiogenesis after myocardial ischemia reperfusion injury.
Masumura M, Watanabe R, Nagashima A, Ogawa M, Suzuki J, Shichiri M, Komuro I, Isobe M
Expert opinion on therapeutic targets17(9)1003-9(2013 Sep)

Salusin-? in paraventricular nucleus increases blood pressure and sympathetic outflow via vasopressin in hypertensive rats.
Chen WW, Sun HJ, Zhang F, Zhou YB, Xiong XQ, Wang JJ, Zhu GQ
Cardiovascular research98(3)344-51(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

salusin-beta may be important in the vascular smooth muscle cell migration and neointima of some vascular diseases.
Title: Salusin-? Promotes Vascular Smooth Muscle Cell Migration and Intimal Hyperplasia After Vascular Injury via ROS/NF?B/MMP-9 Pathway.

Salusin-beta suppresses angiogenesis which is critical in the development of cardiac remodeling following ischemia reperfusion injury.
Title: Anti-salusin-? antibody enhances angiogenesis after myocardial ischemia reperfusion injury.

Salusin-beta in the paraventricular nucleus increases blood pressure, heart rate, and sympathetic outflow via both circulating AVP and AVP in the rostral ventrolateral medulla in hypertensive rats.
Title: Salusin-? in paraventricular nucleus increases blood pressure and sympathetic outflow via vasopressin in hypertensive rats.

Salusin-beta may be involved in the regulation of body fluid balance by stimulating neurohypophyseal hormone release from nerve endings by an autocrine/paracrine mechanism.
Title: Chronic osmotic stimuli increase salusin-beta-like immunoreactivity in the rat hypothalamo-neurohypophyseal system: possible involvement of salusin-beta on [Ca2+]i increase and neurohypophyseal hormone release from the axon terminals.

rat salusin is immunologically similar to human salusin-beta and widely expressed, especially in the immune, gastrointestinal, and central nervous systems and mainly in endocrine- and hematopoietic-derived cells
Title: Systemic distribution of salusin expression in the rat.

The following Tor2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tor2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12639 NM_001007744.1
Latest version!		 Rattus norvegicus torsin family 2, member A (Tor2a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
ORa25740 XM_006233981.3
Latest version!		 Rattus norvegicus torsin family 2, member A (Tor2a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
ORa60472 XM_017591883.1
Latest version!		 Rattus norvegicus torsin family 2, member A (Tor2a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12639
Clone ID Related Accession (Same CDS sequence) NM_001007744.1
Accession Version NM_001007744.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 966bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-27
Organism Rattus norvegicus(Norway rat)
Product torsin-2A precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078943.1. On Nov 29, 2004 this sequence version replaced XM_342415.1. ##Evidence-Data-START## Transcript exon combination :: BC078943.1, EV779984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGTGG CTAGGCACGG CTGCCCGCCC TGGGGCTCGA TCCTCGGGCT GCTCGTGCTG 
GCCTTGGCTG CCGCCGCCGC CTGGGACGTG TCTTTTCTGC GATGCAGTTT AGGGTCCTTC 
TGCGAATGTG ACTTCTGGCC CGACTTGCCT GGTCTGGAAT GTGACCTGGC TCGACACCTA 
GCTGGCCAGC ATTTGGCCAA GGCCCTGGTG GTGAAGTCAT TGAAGGCCTT TGTACAGGAC 
CCAGCCCCCA GCAAGCCATT GGTCCTTTCC CTGCACGGCT GGACAGGCAC TGGGAAGTCC 
TATGTGAGCT CCCTGCTGGC GCAGTATCTC TTCCGGGGTG GCCTTCGCAG CCCTCATGTC 
CATCACTTCT CTCCTATCAT CCACTTCCCA CATCCCAGCC ACACTGAGCA GTACAAGAAT 
GAGCTCAAGA GCTGGGTCCA GGGGAACCTC ACTGCCTGTG GCCGGTCTCT TTTCCTCTTC 
GATGAGATGG ACAAGCTGCC CCCTGGCCTG ATGGAAGTCC TGAAGCCCTT CCTGGGCCCT 
TCTTGGGTTG TGTATGGGAC CAACTATCGC AAAGCCATTT TCATCTTTAT CAGCAATACT 
GGAGGTGAGC AGATCAACCA GGTGGCCCTG GAGGCATGGC GCAGCCGTAG GGACAGGGAA 
GAAATCAGCC TACAGGAGGT GGAGCCAGCA GTGTCCCAAG CTGTGTTGGA CAACCCTCAC 
CATGGCTTCT GGCGGTCTGG CATCATGGAG GAGCAGCTGC TGGATGCTGT GGTCCCCTTC 
CTCCCCCTCC AGCGGCATCA TGTGCGCCAC TGTGTGCTCA ATGAGCTGGC TCAGCTGGGT 
TTGGAGCCCC GAGAGGAGGT GGTTCAGGCT GTGCTGGACA GTACCACCTA CTTCCCTGAG 
GAGGAGCAGC TCTTCTCCTC CAATGGCTGC AAGACAGTGG CATCCCGAAT CACATTTTTC 
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001007745.1
CDS51..1016
Translation

Target ORF information:

RefSeq Version NM_001007744.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus torsin family 2, member A (Tor2a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001007744.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGTGG CTAGGCACGG CTGCCCGCCC TGGGGCTCGA TCCTCGGGCT GCTCGTGCTG 
GCCTTGGCTG CCGCCGCCGC CTGGGACGTG TCTTTTCTGC GATGCAGTTT AGGGTCCTTC 
TGCGAATGTG ACTTCTGGCC CGACTTGCCT GGTCTGGAAT GTGACCTGGC TCGACACCTA 
GCTGGCCAGC ATTTGGCCAA GGCCCTGGTG GTGAAGTCAT TGAAGGCCTT TGTACAGGAC 
CCAGCCCCCA GCAAGCCATT GGTCCTTTCC CTGCACGGCT GGACAGGCAC TGGGAAGTCC 
TATGTGAGCT CCCTGCTGGC GCAGTATCTC TTCCGGGGTG GCCTTCGCAG CCCTCATGTC 
CATCACTTCT CTCCTATCAT CCACTTCCCA CATCCCAGCC ACACTGAGCA GTACAAGAAT 
GAGCTCAAGA GCTGGGTCCA GGGGAACCTC ACTGCCTGTG GCCGGTCTCT TTTCCTCTTC 
GATGAGATGG ACAAGCTGCC CCCTGGCCTG ATGGAAGTCC TGAAGCCCTT CCTGGGCCCT 
TCTTGGGTTG TGTATGGGAC CAACTATCGC AAAGCCATTT TCATCTTTAT CAGCAATACT 
GGAGGTGAGC AGATCAACCA GGTGGCCCTG GAGGCATGGC GCAGCCGTAG GGACAGGGAA 
GAAATCAGCC TACAGGAGGT GGAGCCAGCA GTGTCCCAAG CTGTGTTGGA CAACCCTCAC 
CATGGCTTCT GGCGGTCTGG CATCATGGAG GAGCAGCTGC TGGATGCTGT GGTCCCCTTC 
CTCCCCCTCC AGCGGCATCA TGTGCGCCAC TGTGTGCTCA ATGAGCTGGC TCAGCTGGGT 
TTGGAGCCCC GAGAGGAGGT GGTTCAGGCT GTGCTGGACA GTACCACCTA CTTCCCTGAG 
GAGGAGCAGC TCTTCTCCTC CAATGGCTGC AAGACAGTGG CATCCCGAAT CACATTTTTC 
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25740
Clone ID Related Accession (Same CDS sequence) XM_006233981.3
Accession Version XM_006233981.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 942bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product torsin-2A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233981.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCTCTTAA CAATCTTTAC CCGTGACCAT GGCAACGCGG GACTGGGCGG TTTGCTGGCT 
CCCCCAGGAG GCCTATTTGC GCTACTGCTG CCGGACAACC GGTCGGGTTT TAGAGCCCCG 
AAGAAAGGTC TGGAATGTGA CCTGGCTCGA CACCTAGCTG GCCAGCATTT GGCCAAGGCC 
CTGGTGGTGA AGTCATTGAA GGCCTTTGTA CAGGACCCAG CCCCCAGCAA GCCATTGGTC 
CTTTCCCTGC ACGGCTGGAC AGGCACTGGG AAGTCCTATG TGAGCTCCCT GCTGGCGCAG 
TATCTCTTCC GGGGTGGCCT TCGCAGCCCT CATGTCCATC ACTTCTCTCC TATCATCCAC 
TTCCCACATC CCAGCCACAC TGAGCAGTAC AAGAATGAGC TCAAGAGCTG GGTCCAGGGG 
AACCTCACTG CCTGTGGCCG GTCTCTTTTC CTCTTCGATG AGATGGACAA GCTGCCCCCT 
GGCCTGATGG AAGTCCTGAA GCCCTTCCTG GGCCCTTCTT GGGTTGTGTA TGGGACCAAC 
TATCGCAAAG CCATTTTCAT CTTTATCAGC AATACTGGAG GTGAGCAGAT CAACCAGGTG 
GCCCTGGAGG CATGGCGCAG CCGTAGGGAC AGGGAAGAAA TCAGCCTACA GGAGGTGGAG 
CCAGCAGTGT CCCAAGCTGT GTTGGACAAC CCTCACCATG GCTTCTGGCG GTCTGGCATC 
ATGGAGGAGC AGCTGCTGGA TGCTGTGGTC CCCTTCCTCC CCCTCCAGCG GCATCATGTG 
CGCCACTGTG TGCTCAATGA GCTGGCTCAG CTGGGTTTGG AGCCCCGAGA GGAGGTGGTT 
CAGGCTGTGC TGGACAGTAC CACCTACTTC CCTGAGGAGG AGCAGCTCTT CTCCTCCAAT 
GGCTGCAAGA CAGTGGCATC CCGAATCACA TTTTTCCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006234043.1
CDS706..1647
Translation

Target ORF information:

RefSeq Version XM_006233981.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus torsin family 2, member A (Tor2a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233981.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCTCTTAA CAATCTTTAC CCGTGACCAT GGCAACGCGG GACTGGGCGG TTTGCTGGCT 
CCCCCAGGAG GCCTATTTGC GCTACTGCTG CCGGACAACC GGTCGGGTTT TAGAGCCCCG 
AAGAAAGGTC TGGAATGTGA CCTGGCTCGA CACCTAGCTG GCCAGCATTT GGCCAAGGCC 
CTGGTGGTGA AGTCATTGAA GGCCTTTGTA CAGGACCCAG CCCCCAGCAA GCCATTGGTC 
CTTTCCCTGC ACGGCTGGAC AGGCACTGGG AAGTCCTATG TGAGCTCCCT GCTGGCGCAG 
TATCTCTTCC GGGGTGGCCT TCGCAGCCCT CATGTCCATC ACTTCTCTCC TATCATCCAC 
TTCCCACATC CCAGCCACAC TGAGCAGTAC AAGAATGAGC TCAAGAGCTG GGTCCAGGGG 
AACCTCACTG CCTGTGGCCG GTCTCTTTTC CTCTTCGATG AGATGGACAA GCTGCCCCCT 
GGCCTGATGG AAGTCCTGAA GCCCTTCCTG GGCCCTTCTT GGGTTGTGTA TGGGACCAAC 
TATCGCAAAG CCATTTTCAT CTTTATCAGC AATACTGGAG GTGAGCAGAT CAACCAGGTG 
GCCCTGGAGG CATGGCGCAG CCGTAGGGAC AGGGAAGAAA TCAGCCTACA GGAGGTGGAG 
CCAGCAGTGT CCCAAGCTGT GTTGGACAAC CCTCACCATG GCTTCTGGCG GTCTGGCATC 
ATGGAGGAGC AGCTGCTGGA TGCTGTGGTC CCCTTCCTCC CCCTCCAGCG GCATCATGTG 
CGCCACTGTG TGCTCAATGA GCTGGCTCAG CTGGGTTTGG AGCCCCGAGA GGAGGTGGTT 
CAGGCTGTGC TGGACAGTAC CACCTACTTC CCTGAGGAGG AGCAGCTCTT CTCCTCCAAT 
GGCTGCAAGA CAGTGGCATC CCGAATCACA TTTTTCCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa60472
Clone ID Related Accession (Same CDS sequence) XM_017591883.1
Accession Version XM_017591883.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product torsin-2A isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
ATGGACAAGC TGCCCCCTGG CCTGATGGAA GTCCTGAAGC CCTTCCTGGG CCCTTCTTGG 
GTTGTGTATG GGACCAACTA TCGCAAAGCC ATTTTCATCT TTATCAGCAA TACTGGAGGT 
GAGCAGATCA ACCAGGTGGC CCTGGAGGCA TGGCGCAGCC GTAGGGACAG GGAAGAAATC 
AGCCTACAGG AGGTGGAGCC AGCAGTGTCC CAAGCTGTGT TGGACAACCC TCACCATGGC 
TTCTGGCGGT CTGGCATCAT GGAGGAGCAG CTGCTGGATG CTGTGGTCCC CTTCCTCCCC 
CTCCAGCGGC ATCATGTGCG CCACTGTGTG CTCAATGAGC TGGCTCAGCT GGGTTTGGAG 
CCCCGAGAGG AGGTGGTTCA GGCTGTGCTG GACAGTACCA CCTACTTCCC TGAGGAGGAG 
CAGCTCTTCT CCTCCAATGG CTGCAAGACA GTGGCATCCC GAATCACATT TTTCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017447372.1
CDS1008..1487
Translation

Target ORF information:

RefSeq Version XM_017591883.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus torsin family 2, member A (Tor2a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017591883.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGACAAGC TGCCCCCTGG CCTGATGGAA GTCCTGAAGC CCTTCCTGGG CCCTTCTTGG 
GTTGTGTATG GGACCAACTA TCGCAAAGCC ATTTTCATCT TTATCAGCAA TACTGGAGGT 
GAGCAGATCA ACCAGGTGGC CCTGGAGGCA TGGCGCAGCC GTAGGGACAG GGAAGAAATC 
AGCCTACAGG AGGTGGAGCC AGCAGTGTCC CAAGCTGTGT TGGACAACCC TCACCATGGC 
TTCTGGCGGT CTGGCATCAT GGAGGAGCAG CTGCTGGATG CTGTGGTCCC CTTCCTCCCC 
CTCCAGCGGC ATCATGTGCG CCACTGTGTG CTCAATGAGC TGGCTCAGCT GGGTTTGGAG 
CCCCGAGAGG AGGTGGTTCA GGCTGTGCTG GACAGTACCA CCTACTTCCC TGAGGAGGAG 
CAGCTCTTCT CCTCCAATGG CTGCAAGACA GTGGCATCCC GAATCACATT TTTCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Silencing salusin-? attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.
Scientific reports743259(2017 Feb)
Ren XS,Ling L,Zhou B,Han Y,Zhou YB,Chen Q,Li YH,Kang YM,Zhu GQ


book

Salusin-? Promotes Vascular Smooth Muscle Cell Migration and Intimal Hyperplasia After Vascular Injury via ROS/NF?B/MMP-9 Pathway.
Antioxidants & redox signaling24(18)1045-57(2016 Jun)
Sun HJ,Zhao MX,Ren XS,Liu TY,Chen Q,Li YH,Kang YM,Wang JJ,Zhu GQ


book

Anti-salusin-? antibody enhances angiogenesis after myocardial ischemia reperfusion injury.
Expert opinion on therapeutic targets17(9)1003-9(2013 Sep)
Masumura M,Watanabe R,Nagashima A,Ogawa M,Suzuki J,Shichiri M,Komuro I,Isobe M


book

Salusin-? in paraventricular nucleus increases blood pressure and sympathetic outflow via vasopressin in hypertensive rats.
Cardiovascular research98(3)344-51(2013 Jun)
Chen WW,Sun HJ,Zhang F,Zhou YB,Xiong XQ,Wang JJ,Zhu GQ