Thsd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thsd1
Entrez Gene ID	364630
Full Name	thrombospondin type 1 domain containing 1
General protein information	Preferred Names thrombospondin type 1 domain containing 1 Names thrombospondin type-1 domain-containing protein 1 thrombospondin, type I, domain 1 thrombospondin, type I, domain containing 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006253357.3	XP_006253419.1	thrombospondin type-1 domain-containing protein 1 isoform X1
NM_001108878.1	NP_001102348.1	thrombospondin type-1 domain-containing protein 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THSD1	XP_509797.4
Canis lupus familiaris (dog)	THSD1	XP_534485.2
Homo sapiens (human)	THSD1	NP_061146.1
	THSD1	XP_002800815.1
Mus musculus (house mouse)	Thsd1	NP_062522.1
Xenopus tropicalis (tropical clawed frog)	thsd1	XP_004912028.1
Rattus norvegicus (Norway rat)	Thsd1	NP_001102348.1
Gallus gallus (chicken)	THSD1	XP_417078.1
Bos taurus (cattle)	THSD1	NP_001014967.1
Danio rerio (zebrafish)	LOC797520	XP_001337990.3

Gene Ontology
Bibliography

Related articles in PubMed

Effects of PI3-k/Akt short hairpin RNA on proliferation, fibronectin production and synthesis of thrombospondin-1 and transforming growth factor-beta1 in glomerular mesangial cells induced by sublytic C5b-9 complexes.
Gao L, Zhang Y, Qiu W, Xu W, Feng X, Ren J, Jiang X, Wang H, Zhao D, Wang Y
Cell proliferation42(1)83-93(2009 Feb)

Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain.
Takayanagi S, Hiroyama T, Yamazaki S, Nakajima T, Morita Y, Usui J, Eto K, Motohashi T, Shiomi K, Keino-Masu K, Masu M, Oike Y, Mori S, Yoshida N, Iwama A, Nakauchi H
Blood107(11)4317-25(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C5b-9 can promote proliferation of GMCs and secretion of fibronectin as well as synthesis of thrombospondin-1 and transforming growth factor-beta(1)
Title: Effects of PI3-k/Akt short hairpin RNA on proliferation, fibronectin production and synthesis of thrombospondin-1 and transforming growth factor-beta1 in glomerular mesangial cells induced by sublytic C5b-9 complexes.

The following Thsd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thsd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08548	NM_001108878.1 Latest version!	Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa37514	XM_006253357.3 Latest version!	Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thsd1 ( NM_001108878.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102348.1 Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), mRNA.

Price & Availability↑

CloneID	ORa08548
Clone ID Related Accession (Same CDS sequence)	NM_001108878.1
Accession Version	NM_001108878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	thrombospondin type-1 domain-containing protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473970.2. On or before Oct 4, 2007 this sequence version replaced XM_344542.3, XM_001064856.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAACCCA TGTTGAAAGA CTTTTCAAAT CTCTTGCTGG TGGTGCTCTG TGACTATGTC 
CTTGGAGAAG CCGACTACCT CGTCTTACGA GAGCCAGTCC ACGTGGCGCT GAGCGACCGA 
ACGGTGTCAG TGGGTTTCCA CTACCTCGGT GACGTCAACG GGACACTGAG GAATGTGACT 
GTCATGCTGT GGGAGGCCAA CACCAACCGG ACTCTTACCA CTAAGTACCT CCTGACCAAC 
CAGGCCCAGG GAACACTCCA GTTTGAATGC TTCTACTTCA AGGAGGCTGG TGACTACTGG 
TTTGTGATGA TCCCAGAAGA GACAGACAAT GGCACACGAG TTCCCCTCTG GGAGAAAAGT 
GCCTCTCTGA AGGTCGAATG GCCCGTCTTT CACATCGATT TAAATAGGAC AGCCAAGGCG 
GCAGAAGGCA CCTTCCAAGT GGGTATTTTT ACCTCCCAAC CGCTCTGCCT GTTTCCTGTG 
GACAAGCCAG ACCTGCTGGT GGATGTCATC TTCAAGGACA GCCTTCCTGA GGCAAGAACA 
AGTTTGGGAC AGCCGCTGGA GATCCGAGCC AGCAAAAGGA CAAGACTCAC TCAAGGCCAG 
TGGGTCGAGT TTGGCTGTGC ACCCCTAGGG GTGGAAGCCT ACGTTACGGT GATGCTGAGG 
CTTTTGGGTC AAGACTCAGT CATTGCTTCG ACGGGACCTA TTGACCTGGC CCAAAAGTTC 
GGATACAAAT TGATGATGGC ACCGGAAGTC AGGTGTGAGT CTGTCCTGGA GGTGATGGTA 
CTGCCACCTC CTTGTGTCTT TGTCCAAGGA GTGCTGGCTG TTTACAAAGA AGCCCCCAAA 
GCCCCGGAGG AGAGGACCTT CCAGATGGCT GAAAACAGGC TGCCCCTGGG AGAGAGGAGA 
TCGGTGTTTA ACTGCACTTT GTTTGATGTG GGGAAGAACA AGTATTGCTT TAACTTTGGA 
ATTTTGAAGA AAAGCCATTT TTCTGCAAAG GGATGCATGC TAATTCAGAG AAATATAGAA 
ACTTGGGGAC CATGGCAGCC GTGGAGCCCG TGTAGCACCA CGTGCGGGGA TGCTGTCCGA 
GAGCGTCGCC GCACGTGCGT CACTTCCTTC CCCTCCAGAC CCAGCTGCTC TGGAAAGCCC 
TCAGAGACCT CTCCATGCTC CCTGGAGGAG TGTGCTGTTT TCCGGCCACC AGACCCATCG 
CCGGACCCGG AGAAGTACAA CAACGTGGTG ACCGTCACAG GGATATCCCT GTGCTTGTTC 
ATCATCTTTG CCACCGTGCT CATCACCCTC TGGAGGAGAT TCGGCAGAGC CCCCAAATGC 
AGCACGCCGG CCCGCCACAA CTCCATCCAT TCCCCCGGCT TCCGGAAGAA CTCGGATGAG 
GAGAACATCT GCGAGCTGAG CGAGCCTCGC GGAAGCTTCT CGGATGCGGG GGACGGGCCC 
AGGGGAAGCC CAGGGGACAC GGGCATCCCG TTGACTTACC GGTGCAGTGC GTCAGCGCCT 
CCTGAGGACG AGGCCTCCGG CAGTGAGAGC TTTCAGTCCA ACGCTCAGAA GATCATCCCG 
CCCCTGTTTA GCTACCGCCT GGCCCAGCAG CAGCTGAAGG AGATGAAGAA GAAAGGGCTG 
ACAGAGACCA CCAAAGTGTA CCACGTGTCT CAGAGCCCCC TGACAGACAC GGTAGTGGAT 
GCCACTGCCA GCCCCCCCTT AGACCTGGAG TGCCCCGAAG AGGCAGCAGC AAGCAAGTTC 
CGGATCAAGT CTCCTTTTCT GGACCAGCCT GGGGCAGGTC CCGGGGAAAG GCCCCCCTCC 
AGGCTGGATG GCGTCCTGCC TCCGCCCAGC TGTGCGGTCA GCCCCAGCCA GACGCTGATC 
CGCAAGTCCC AGATGAGGTC GACTGGTGGG AGAGATGGCT CGTCGGAGCG GGGCCACTGC 
AGAAGTTCCC TCTTCCGGAG GACTGCTAGT TTTCACGAAA CCAAGCAGGC TCGCCCTTTC 
AGGGAGAGGA GTTTGTCAGC CCTGACTCCC CGCCAGGCCC CCGCCTACAG TTCCAGGATG 
AGGACCTGGG AGCAGATGGA GGATAGGGGT CGGCCTCCCA GTCGAAGTGC TCACCTGCTT 
CCAGAGAGGC CGGAACACTT CCAAGGGGCG GCAGGTCGGG CCAGCAGTCC TTTGGGTCCA 
CTCTCCAAAT CCTACACTGT GGGGCATCCC AGGAGGAAAC CCGACCCGGG GGATCGCCAG 
GCTGGGTTGG TGGCAGGAGC TGCTGCGGAG AAAATGGAGC CTCACCGAGC TCACAGGGGA 
CCGTCCCCCA GTCACAGGAG TGCGTCAAGG AAGCAGACTT CCCCCGTTTT CCTCAAGGAC 
AGCTACCAGA AAGTCAGTCA GCTTAGCCCT TCTCACTTCA GGAAAGATAA ATGCCAGAGC 
TTCCCCATCC ACCCTGAGTT CGCCTTCTAC GACAACACCT CTTTCCGCCT CACCGAGGCT 
GAGCAGAGAA TGCTGGACCT CCCGGGATAC TTTGGCTCCA ACGAAGAGGA CGAAACCACT 
AGTACACTGA GCGTGGAGAA GTTAGTAATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102348.1
CDS	200..2752
Translation	MKPMLKDFSNLLLVVLCDYVLGEADYLVLREPVHVALSDRTVSVGFHYLGDVNGTLRNVTVMLWEANTNRTLTTKYLLTNQAQGTLQFECFYFKEAGDYWFVMIPEETDNGTRVPLWEKSASLKVEWPVFHIDLNRTAKAAEGTFQVGIFTSQPLCLFPVDKPDLLVDVIFKDSLPEARTSLGQPLEIRASKRTRLTQGQWVEFGCAPLGVEAYVTVMLRLLGQDSVIASTGPIDLAQKFGYKLMMAPEVRCESVLEVMVLPPPCVFVQGVLAVYKEAPKAPEERTFQMAENRLPLGERRSVFNCTLFDVGKNKYCFNFGILKKSHFSAKGCMLIQRNIETWGPWQPWSPCSTTCGDAVRERRRTCVTSFPSRPSCSGKPSETSPCSLEECAVFRPPDPSPDPEKYNNVVTVTGISLCLFIIFATVLITLWRRFGRAPKCSTPARHNSIHSPGFRKNSDEENICELSEPRGSFSDAGDGPRGSPGDTGIPLTYRCSASAPPEDEASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTVVDATASPPLDLECPEEAAASKFRIKSPFLDQPGAGPGERPPSRLDGVLPPPSCAVSPSQTLIRKSQMRSTGGRDGSSERGHCRSSLFRRTASFHETKQARPFRERSLSALTPRQAPAYSSRMRTWEQMEDRGRPPSRSAHLLPERPEHFQGAAGRASSPLGPLSKSYTVGHPRRKPDPGDRQAGLVAGAAAEKMEPHRAHRGPSPSHRSASRKQTSPVFLKDSYQKVSQLSPSHFRKDKCQSFPIHPEFAFYDNTSFRLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	NM_001108878.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108878.1

ORF Insert Sequence:

ATGAAACCCA TGTTGAAAGA CTTTTCAAAT CTCTTGCTGG TGGTGCTCTG TGACTATGTC 
CTTGGAGAAG CCGACTACCT CGTCTTACGA GAGCCAGTCC ACGTGGCGCT GAGCGACCGA 
ACGGTGTCAG TGGGTTTCCA CTACCTCGGT GACGTCAACG GGACACTGAG GAATGTGACT 
GTCATGCTGT GGGAGGCCAA CACCAACCGG ACTCTTACCA CTAAGTACCT CCTGACCAAC 
CAGGCCCAGG GAACACTCCA GTTTGAATGC TTCTACTTCA AGGAGGCTGG TGACTACTGG 
TTTGTGATGA TCCCAGAAGA GACAGACAAT GGCACACGAG TTCCCCTCTG GGAGAAAAGT 
GCCTCTCTGA AGGTCGAATG GCCCGTCTTT CACATCGATT TAAATAGGAC AGCCAAGGCG 
GCAGAAGGCA CCTTCCAAGT GGGTATTTTT ACCTCCCAAC CGCTCTGCCT GTTTCCTGTG 
GACAAGCCAG ACCTGCTGGT GGATGTCATC TTCAAGGACA GCCTTCCTGA GGCAAGAACA 
AGTTTGGGAC AGCCGCTGGA GATCCGAGCC AGCAAAAGGA CAAGACTCAC TCAAGGCCAG 
TGGGTCGAGT TTGGCTGTGC ACCCCTAGGG GTGGAAGCCT ACGTTACGGT GATGCTGAGG 
CTTTTGGGTC AAGACTCAGT CATTGCTTCG ACGGGACCTA TTGACCTGGC CCAAAAGTTC 
GGATACAAAT TGATGATGGC ACCGGAAGTC AGGTGTGAGT CTGTCCTGGA GGTGATGGTA 
CTGCCACCTC CTTGTGTCTT TGTCCAAGGA GTGCTGGCTG TTTACAAAGA AGCCCCCAAA 
GCCCCGGAGG AGAGGACCTT CCAGATGGCT GAAAACAGGC TGCCCCTGGG AGAGAGGAGA 
TCGGTGTTTA ACTGCACTTT GTTTGATGTG GGGAAGAACA AGTATTGCTT TAACTTTGGA 
ATTTTGAAGA AAAGCCATTT TTCTGCAAAG GGATGCATGC TAATTCAGAG AAATATAGAA 
ACTTGGGGAC CATGGCAGCC GTGGAGCCCG TGTAGCACCA CGTGCGGGGA TGCTGTCCGA 
GAGCGTCGCC GCACGTGCGT CACTTCCTTC CCCTCCAGAC CCAGCTGCTC TGGAAAGCCC 
TCAGAGACCT CTCCATGCTC CCTGGAGGAG TGTGCTGTTT TCCGGCCACC AGACCCATCG 
CCGGACCCGG AGAAGTACAA CAACGTGGTG ACCGTCACAG GGATATCCCT GTGCTTGTTC 
ATCATCTTTG CCACCGTGCT CATCACCCTC TGGAGGAGAT TCGGCAGAGC CCCCAAATGC 
AGCACGCCGG CCCGCCACAA CTCCATCCAT TCCCCCGGCT TCCGGAAGAA CTCGGATGAG 
GAGAACATCT GCGAGCTGAG CGAGCCTCGC GGAAGCTTCT CGGATGCGGG GGACGGGCCC 
AGGGGAAGCC CAGGGGACAC GGGCATCCCG TTGACTTACC GGTGCAGTGC GTCAGCGCCT 
CCTGAGGACG AGGCCTCCGG CAGTGAGAGC TTTCAGTCCA ACGCTCAGAA GATCATCCCG 
CCCCTGTTTA GCTACCGCCT GGCCCAGCAG CAGCTGAAGG AGATGAAGAA GAAAGGGCTG 
ACAGAGACCA CCAAAGTGTA CCACGTGTCT CAGAGCCCCC TGACAGACAC GGTAGTGGAT 
GCCACTGCCA GCCCCCCCTT AGACCTGGAG TGCCCCGAAG AGGCAGCAGC AAGCAAGTTC 
CGGATCAAGT CTCCTTTTCT GGACCAGCCT GGGGCAGGTC CCGGGGAAAG GCCCCCCTCC 
AGGCTGGATG GCGTCCTGCC TCCGCCCAGC TGTGCGGTCA GCCCCAGCCA GACGCTGATC 
CGCAAGTCCC AGATGAGGTC GACTGGTGGG AGAGATGGCT CGTCGGAGCG GGGCCACTGC 
AGAAGTTCCC TCTTCCGGAG GACTGCTAGT TTTCACGAAA CCAAGCAGGC TCGCCCTTTC 
AGGGAGAGGA GTTTGTCAGC CCTGACTCCC CGCCAGGCCC CCGCCTACAG TTCCAGGATG 
AGGACCTGGG AGCAGATGGA GGATAGGGGT CGGCCTCCCA GTCGAAGTGC TCACCTGCTT 
CCAGAGAGGC CGGAACACTT CCAAGGGGCG GCAGGTCGGG CCAGCAGTCC TTTGGGTCCA 
CTCTCCAAAT CCTACACTGT GGGGCATCCC AGGAGGAAAC CCGACCCGGG GGATCGCCAG 
GCTGGGTTGG TGGCAGGAGC TGCTGCGGAG AAAATGGAGC CTCACCGAGC TCACAGGGGA 
CCGTCCCCCA GTCACAGGAG TGCGTCAAGG AAGCAGACTT CCCCCGTTTT CCTCAAGGAC 
AGCTACCAGA AAGTCAGTCA GCTTAGCCCT TCTCACTTCA GGAAAGATAA ATGCCAGAGC 
TTCCCCATCC ACCCTGAGTT CGCCTTCTAC GACAACACCT CTTTCCGCCT CACCGAGGCT 
GAGCAGAGAA TGCTGGACCT CCCGGGATAC TTTGGCTCCA ACGAAGAGGA CGAAACCACT 
AGTACACTGA GCGTGGAGAA GTTAGTAATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd1 ( XM_006253357.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253419.1 Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37514
Clone ID Related Accession (Same CDS sequence)	XM_006253357.3
Accession Version	XM_006253357.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	thrombospondin type-1 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCCA TGTTGAAAGA CTTTTCAAAT CTCTTGCTGG TGGTGCTCTG TGACTATGTC 
CTTGGAGAAG CCGACTACCT CGTCTTACGA GAGCCAGTCC ACGTGGCGCT GAGCGACCGA 
ACGGTGTCAG TGGGTTTCCA CTACCTCGGT GACGTCAACG GGACACTGAG GAATGTGACT 
GTCATGCTGT GGGAGGCCAA CACCAACCGG ACTCTTACCA CTAAGTACCT CCTGACCAAC 
CAGGCCCAGG GAACACTCCA GTTTGAATGC TTCTACTTCA AGGAGGCTGG TGACTACTGG 
TTTGTGATGA TCCCAGAAGA GACAGACAAT GGCACACGAG TTCCCCTCTG GGAGAAAAGT 
GCCTCTCTGA AGGTCGAATG GCCCGTCTTT CACATCGATT TAAATAGGAC AGCCAAGGCG 
GCAGAAGGCA CCTTCCAAGT GGGTATTTTT ACCTCCCAAC CGCTCTGCCT GTTTCCTGTG 
GACAAGCCAG ACCTGCTGGT GGATGTCATC TTCAAGGACA GCCTTCCTGA GGCAAGAACA 
AGTTTGGGAC AGCCGCTGGA GATCCGAGCC AGCAAAAGGA CAAGACTCAC TCAAGGCCAG 
TGGGTCGAGT TTGGCTGTGC ACCCCTAGGG GTGGAAGCCT ACGTTACGGT GATGCTGAGG 
CTTTTGGGTC AAGACTCAGT CATTGCTTCG ACGGGACCTA TTGACCTGGC CCAAAAGTTC 
GGATACAAAT TGATGATGGC ACCGGAAGTC AGGTGTGAGT CTGTCCTGGA GGTGATGGTA 
CTGCCACCTC CTTGTGTCTT TGTCCAAGGA GTGCTGGCTG TTTACAAAGA AGCCCCCAAA 
GCCCCGGAGG AGAGGACCTT CCAGATGGCT GAAAACAGGC TGCCCCTGGG AGAGAGGAGA 
TCGGTGTTTA ACTGCACTTT GTTTGATGTG GGGAAGAACA AGTATTGCTT TAACTTTGGA 
ATTTTGAAGA AAAGCCATTT TTCTGCAAAG GGATGCATGC TAATTCAGAG AAATATAGTT 
TTCCGGCCAC CAGACCCATC GCCGGACCCG GAGAAGTACA ACAACGTGGT GACCGTCACA 
GGGATATCCC TGTGCTTGTT CATCATCTTT GCCACCGTGC TCATCACCCT CTGGAGGAGA 
TTCGGCAGAG CCCCCAAATG CAGCACGCCG GCCCGCCACA ACTCCATCCA TTCCCCCGGC 
TTCCGGAAGA ACTCGGATGA GGAGAACATC TGCGAGCTGA GCGAGCCTCG CGGAAGCTTC 
TCGGATGCGG GGGACGGGCC CAGGGGAAGC CCAGGGGACA CGGGCATCCC GTTGACTTAC 
CGGTGCAGTG CGTCAGCGCC TCCTGAGGAC GAGGCCTCCG GCAGTGAGAG CTTTCAGTCC 
AACGCTCAGA AGATCATCCC GCCCCTGTTT AGCTACCGCC TGGCCCAGCA GCAGCTGAAG 
GAGATGAAGA AGAAAGGGCT GACAGAGACC ACCAAAGTGT ACCACGTGTC TCAGAGCCCC 
CTGACAGACA CGGTAGTGGA TGCCACTGCC AGCCCCCCCT TAGACCTGGA GTGCCCCGAA 
GAGGCAGCAG CAAGCAAGTT CCGGATCAAG TCTCCTTTTC TGGACCAGCC TGGGGCAGGT 
CCCGGGGAAA GGCCCCCCTC CAGGCTGGAT GGCGTCCTGC CTCCGCCCAG CTGTGCGGTC 
AGCCCCAGCC AGACGCTGAT CCGCAAGTCC CAGATGAGGT CGACTGGTGG GAGAGATGGC 
TCGTCGGAGC GGGGCCACTG CAGAAGTTCC CTCTTCCGGA GGACTGCTAG TTTTCACGAA 
ACCAAGCAGG CTCGCCCTTT CAGGGAGAGG AGTTTGTCAG CCCTGACTCC CCGCCAGGCC 
CCCGCCTACA GTTCCAGGAT GAGGACCTGG GAGCAGATGG AGGATAGGGG TCGGCCTCCC 
AGTCGAAGTG CTCACCTGCT TCCAGAGAGG CCGGAACACT TCCAAGGGGC GGCAGGTCGG 
GCCAGCAGTC CTTTGGGTCC ACTCTCCAAA TCCTACACTG TGGGGCATCC CAGGAGGAAA 
CCCGACCCGG GGGATCGCCA GGCTGGGTTG GTGGCAGGAG CTGCTGCGGA GAAAATGGAG 
CCTCACCGAG CTCACAGGGG ACCGTCCCCC AGTCACAGGA GTGCGTCAAG GAAGCAGACT 
TCCCCCGTTT TCCTCAAGGA CAGCTACCAG AAAGTCAGTC AGCTTAGCCC TTCTCACTTC 
AGGAAAGATA AATGCCAGAG CTTCCCCATC CACCCTGAGT TCGCCTTCTA CGACAACACC 
TCTTTCCGCC TCACCGAGGC TGAGCAGAGA ATGCTGGACC TCCCGGGATA CTTTGGCTCC 
AACGAAGAGG ACGAAACCAC TAGTACACTG AGCGTGGAGA AGTTAGTAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253419.1
CDS	1864..4257
Translation	MKPMLKDFSNLLLVVLCDYVLGEADYLVLREPVHVALSDRTVSVGFHYLGDVNGTLRNVTVMLWEANTNRTLTTKYLLTNQAQGTLQFECFYFKEAGDYWFVMIPEETDNGTRVPLWEKSASLKVEWPVFHIDLNRTAKAAEGTFQVGIFTSQPLCLFPVDKPDLLVDVIFKDSLPEARTSLGQPLEIRASKRTRLTQGQWVEFGCAPLGVEAYVTVMLRLLGQDSVIASTGPIDLAQKFGYKLMMAPEVRCESVLEVMVLPPPCVFVQGVLAVYKEAPKAPEERTFQMAENRLPLGERRSVFNCTLFDVGKNKYCFNFGILKKSHFSAKGCMLIQRNIVFRPPDPSPDPEKYNNVVTVTGISLCLFIIFATVLITLWRRFGRAPKCSTPARHNSIHSPGFRKNSDEENICELSEPRGSFSDAGDGPRGSPGDTGIPLTYRCSASAPPEDEASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTVVDATASPPLDLECPEEAAASKFRIKSPFLDQPGAGPGERPPSRLDGVLPPPSCAVSPSQTLIRKSQMRSTGGRDGSSERGHCRSSLFRRTASFHETKQARPFRERSLSALTPRQAPAYSSRMRTWEQMEDRGRPPSRSAHLLPERPEHFQGAAGRASSPLGPLSKSYTVGHPRRKPDPGDRQAGLVAGAAAEKMEPHRAHRGPSPSHRSASRKQTSPVFLKDSYQKVSQLSPSHFRKDKCQSFPIHPEFAFYDNTSFRLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	XM_006253357.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thrombospondin type 1 domain containing 1 (Thsd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253357.3

ORF Insert Sequence:

ATGAAACCCA TGTTGAAAGA CTTTTCAAAT CTCTTGCTGG TGGTGCTCTG TGACTATGTC 
CTTGGAGAAG CCGACTACCT CGTCTTACGA GAGCCAGTCC ACGTGGCGCT GAGCGACCGA 
ACGGTGTCAG TGGGTTTCCA CTACCTCGGT GACGTCAACG GGACACTGAG GAATGTGACT 
GTCATGCTGT GGGAGGCCAA CACCAACCGG ACTCTTACCA CTAAGTACCT CCTGACCAAC 
CAGGCCCAGG GAACACTCCA GTTTGAATGC TTCTACTTCA AGGAGGCTGG TGACTACTGG 
TTTGTGATGA TCCCAGAAGA GACAGACAAT GGCACACGAG TTCCCCTCTG GGAGAAAAGT 
GCCTCTCTGA AGGTCGAATG GCCCGTCTTT CACATCGATT TAAATAGGAC AGCCAAGGCG 
GCAGAAGGCA CCTTCCAAGT GGGTATTTTT ACCTCCCAAC CGCTCTGCCT GTTTCCTGTG 
GACAAGCCAG ACCTGCTGGT GGATGTCATC TTCAAGGACA GCCTTCCTGA GGCAAGAACA 
AGTTTGGGAC AGCCGCTGGA GATCCGAGCC AGCAAAAGGA CAAGACTCAC TCAAGGCCAG 
TGGGTCGAGT TTGGCTGTGC ACCCCTAGGG GTGGAAGCCT ACGTTACGGT GATGCTGAGG 
CTTTTGGGTC AAGACTCAGT CATTGCTTCG ACGGGACCTA TTGACCTGGC CCAAAAGTTC 
GGATACAAAT TGATGATGGC ACCGGAAGTC AGGTGTGAGT CTGTCCTGGA GGTGATGGTA 
CTGCCACCTC CTTGTGTCTT TGTCCAAGGA GTGCTGGCTG TTTACAAAGA AGCCCCCAAA 
GCCCCGGAGG AGAGGACCTT CCAGATGGCT GAAAACAGGC TGCCCCTGGG AGAGAGGAGA 
TCGGTGTTTA ACTGCACTTT GTTTGATGTG GGGAAGAACA AGTATTGCTT TAACTTTGGA 
ATTTTGAAGA AAAGCCATTT TTCTGCAAAG GGATGCATGC TAATTCAGAG AAATATAGTT 
TTCCGGCCAC CAGACCCATC GCCGGACCCG GAGAAGTACA ACAACGTGGT GACCGTCACA 
GGGATATCCC TGTGCTTGTT CATCATCTTT GCCACCGTGC TCATCACCCT CTGGAGGAGA 
TTCGGCAGAG CCCCCAAATG CAGCACGCCG GCCCGCCACA ACTCCATCCA TTCCCCCGGC 
TTCCGGAAGA ACTCGGATGA GGAGAACATC TGCGAGCTGA GCGAGCCTCG CGGAAGCTTC 
TCGGATGCGG GGGACGGGCC CAGGGGAAGC CCAGGGGACA CGGGCATCCC GTTGACTTAC 
CGGTGCAGTG CGTCAGCGCC TCCTGAGGAC GAGGCCTCCG GCAGTGAGAG CTTTCAGTCC 
AACGCTCAGA AGATCATCCC GCCCCTGTTT AGCTACCGCC TGGCCCAGCA GCAGCTGAAG 
GAGATGAAGA AGAAAGGGCT GACAGAGACC ACCAAAGTGT ACCACGTGTC TCAGAGCCCC 
CTGACAGACA CGGTAGTGGA TGCCACTGCC AGCCCCCCCT TAGACCTGGA GTGCCCCGAA 
GAGGCAGCAG CAAGCAAGTT CCGGATCAAG TCTCCTTTTC TGGACCAGCC TGGGGCAGGT 
CCCGGGGAAA GGCCCCCCTC CAGGCTGGAT GGCGTCCTGC CTCCGCCCAG CTGTGCGGTC 
AGCCCCAGCC AGACGCTGAT CCGCAAGTCC CAGATGAGGT CGACTGGTGG GAGAGATGGC 
TCGTCGGAGC GGGGCCACTG CAGAAGTTCC CTCTTCCGGA GGACTGCTAG TTTTCACGAA 
ACCAAGCAGG CTCGCCCTTT CAGGGAGAGG AGTTTGTCAG CCCTGACTCC CCGCCAGGCC 
CCCGCCTACA GTTCCAGGAT GAGGACCTGG GAGCAGATGG AGGATAGGGG TCGGCCTCCC 
AGTCGAAGTG CTCACCTGCT TCCAGAGAGG CCGGAACACT TCCAAGGGGC GGCAGGTCGG 
GCCAGCAGTC CTTTGGGTCC ACTCTCCAAA TCCTACACTG TGGGGCATCC CAGGAGGAAA 
CCCGACCCGG GGGATCGCCA GGCTGGGTTG GTGGCAGGAG CTGCTGCGGA GAAAATGGAG 
CCTCACCGAG CTCACAGGGG ACCGTCCCCC AGTCACAGGA GTGCGTCAAG GAAGCAGACT 
TCCCCCGTTT TCCTCAAGGA CAGCTACCAG AAAGTCAGTC AGCTTAGCCC TTCTCACTTC 
AGGAAAGATA AATGCCAGAG CTTCCCCATC CACCCTGAGT TCGCCTTCTA CGACAACACC 
TCTTTCCGCC TCACCGAGGC TGAGCAGAGA ATGCTGGACC TCCCGGGATA CTTTGGCTCC 
AACGAAGAGG ACGAAACCAC TAGTACACTG AGCGTGGAGA AGTTAGTAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of PI3-k/Akt short hairpin RNA on proliferation, fibronectin production and synthesis of thrombospondin-1 and transforming growth factor-beta1 in glomerular mesangial cells induced by sublytic C5b-9 complexes.
Cell proliferation42(1)83-93(2009 Feb)
Gao L,Zhang Y,Qiu W,Xu W,Feng X,Ren J,Jiang X,Wang H,Zhao D,Wang Y

Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain.
Blood107(11)4317-25(2006 Jun)
Takayanagi S,Hiroyama T,Yamazaki S,Nakajima T,Morita Y,Usui J,Eto K,Motohashi T,Shiomi K,Keino-Masu K,Masu M,Oike Y,Mori S,Yoshida N,Iwama A,Nakauchi H

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Thsd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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