Sptlc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptlc2
Entrez Gene ID	366697
Full Name	serine palmitoyltransferase, long chain base subunit 2
Synonyms	Pomt2
General protein information	Preferred Names serine palmitoyltransferase, long chain base subunit 2 Names serine palmitoyltransferase 2 protein-O-mannosyltransferase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764823.2	XP_008763045.2	serine palmitoyltransferase 2 isoform X1
NM_001037097.1	NP_001032174.1	serine palmitoyltransferase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPTLC2	XP_510095.3
Caenorhabditis elegans (roundworm)	sptl-2	NP_505065.1
Magnaporthe oryzae (rice blast fungus)	MGG_05197	XP_003712740.1
Homo sapiens (human)	SPTLC2	NP_004854.1
Rattus norvegicus (Norway rat)	Sptlc2	NP_001032174.1
Gallus gallus (chicken)	SPTLC2	NP_001006483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007941	XP_317536.4
Schizosaccharomyces pombe (fission yeast)	lcb2	XP_001713103.1
Arabidopsis thaliana (thale cress)	LCB2	NP_197756.1
Oryza sativa (rice)	Os01g0928700	NP_001045278.1
Oryza sativa (rice)	Os01g0928800	NP_001045279.1
Mus musculus (house mouse)	Sptlc2	NP_035609.1
Kluyveromyces lactis	KLLA0D02134g	XP_453163.1
Oryza sativa (rice)	Os11g0516000	NP_001067972.1
Danio rerio (zebrafish)	sptlc2a	NP_001018455.1
Saccharomyces cerevisiae (baker's yeast)	LCB2	NP_010347.1
Neurospora crassa	NCU00447	XP_956766.1
	LOC707625	XP_001096042.2
Canis lupus familiaris (dog)	SPTLC2	XP_537524.3
Bos taurus (cattle)	SPTLC2	NP_001092551.1
Drosophila melanogaster (fruit fly)	lace	NP_476614.1
Eremothecium gossypii	AGOS_AGR127C	NP_986793.2
Arabidopsis thaliana (thale cress)	SPT1	NP_190447.1
Xenopus tropicalis (tropical clawed frog)	sptlc2	XP_002938915.1

Gene Ontology
Bibliography

Related articles in PubMed

Adipocyte-Specific Deficiency of De Novo Sphingolipid Biosynthesis Leads to Lipodystrophy and Insulin Resistance.
Lee SY, Lee HY, Song JH, Kim GT, Jeon S, Song YJ, Lee JS, Hur JH, Oh HH, Park SY, Shim SM, Yoo HJ, Lee BC, Jiang XC, Choi CS, Park TS
Diabetes66(10)2596-2609(2017 10)

Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation, and Metabolism.
Chaurasia B, Kaddai VA, Lancaster GI, Henstridge DC, Sriram S, Galam DL, Gopalan V, Prakash KN, Velan SS, Bulchand S, Tsong TJ, Wang M, Siddique MM, Yuguang G, Sigmundsson K, Mellet NA, Weir JM, Meikle PJ, Bin M Yassin MS, Shabbir A, Shayman JA, Hirabayashi Y, Shiow ST, Sugii S, Summers SA
Cell metabolism24(6)820-834(2016 Dec)

The Variant p.(Arg183Trp) in SPTLC2 Causes Late-Onset Hereditary Sensory Neuropathy.
Suriyanarayanan S, Auranen M, Toppila J, Paetau A, Shcherbii M, Palin E, Wei Y, Lohioja T, Schlotter-Weigel B, Sch?n U, Abicht A, Rautenstrauss B, Tyynismaa H, Walter MC, Hornemann T, Ylikallio E
Neuromolecular medicine18(1)81-90(2016 Mar)

Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.
Obanda DN, Yu Y, Wang ZQ, Cefalu WT
The Journal of nutritional biochemistry26(7)687-95(2015 Jul)

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Siow D, Sunkara M, Dunn TM, Morris AJ, Wattenberg B
Journal of lipid research56(4)898-908(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that expression of enzymes involved in sphingolipid metabolism in skeletal muscle are regulated by dietary factors; here, caloric restriction down-regulates expression of Spt1 and Spt2 but up-regulates ceramide synthases (Cers1, Cers2).
Title: Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.

marked upregulation of expression of both SPT subunits in actively proliferating cells in balloon-injured vessels(serine palmitoyltransferase 1)(serine palmitoyltransferase 2)
Title: Increased expression of serine palmitoyltransferase (SPT) in balloon-injured rat carotid artery.

The following Sptlc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptlc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09225	NM_001037097.1 Latest version!	Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
ORa61690	XM_008764823.2 Latest version!	Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptlc2 ( NM_001037097.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001032174.1 Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.

Price & Availability↑

CloneID	ORa09225
Clone ID Related Accession (Same CDS sequence)	NM_001037097.1
Accession Version	NM_001037097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	serine palmitoyltransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107662.1. On Nov 5, 2005 this sequence version replaced XM_345708.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107662.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGGCCGG AGCCAGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTG 
AAAAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCAT CGCGGCTGCG 
GGCCAGATTC ATCATATTAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT AGTTGCTGTG CTCACATACG TGGGCTATGG TGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG GATCAGAGAC AACTGGAATC GACCCATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGACAG TCTCATGACT ACAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTTC TCAAGACATA TGGAGCAGGG 
GTGTGCAGCA CTCGCCAGGA AATTGGGAAC CTGGACAAGC ACGAGGAACT AGAGAAACTG 
GTAGCAAGGT TCTTGGGTGT GGAAGCTGCT TTGACCTATG GCATGGGATT TGCGACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCATCCT TGGTTCTAGG AGCCAGACTG TCGGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTGGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGGC CCTGGAAGAA AATCCTAATC CTCGTGGAAG GCATATATAG CATGGAAGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGTGGTGT AGTAGACTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATTGG CGGCAAGAAG GAGCTGATAG ACTACCTACG CACACATTCT 
CACAGTGCTG TGTACGCCAC GTCAATGTCA CCACCTGTGA TGGAGCAGAT CATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT CCAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGGTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGGAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCA 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCGGCTC ATACCAAAGA AATACTTGAC 
ACGGCTTTGA AGGAAATAGA TGAAGTTGGG GATCTGCTGC AACTGAAGTA CTCCCGCCAC 
CGGCCGCTGC CTCTGCTGGA CAGGCCCTTT GACGAGACTA CCTATGAGGA GACAGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032174.1
CDS	101..1783
Translation	MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATIAAAGQIHHITENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKTYGAGVCSTRQEIGNLDKHEELEKLVARFLGVEAALTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKECIQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRPLPLLDRPFDETTYEETED

Target ORF information:

RefSeq Version	NM_001037097.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037097.1

ORF Insert Sequence:

ATGCGGCCGG AGCCAGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTG 
AAAAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCAT CGCGGCTGCG 
GGCCAGATTC ATCATATTAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT AGTTGCTGTG CTCACATACG TGGGCTATGG TGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG GATCAGAGAC AACTGGAATC GACCCATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGACAG TCTCATGACT ACAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTTC TCAAGACATA TGGAGCAGGG 
GTGTGCAGCA CTCGCCAGGA AATTGGGAAC CTGGACAAGC ACGAGGAACT AGAGAAACTG 
GTAGCAAGGT TCTTGGGTGT GGAAGCTGCT TTGACCTATG GCATGGGATT TGCGACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCATCCT TGGTTCTAGG AGCCAGACTG TCGGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTGGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGGC CCTGGAAGAA AATCCTAATC CTCGTGGAAG GCATATATAG CATGGAAGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGTGGTGT AGTAGACTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATTGG CGGCAAGAAG GAGCTGATAG ACTACCTACG CACACATTCT 
CACAGTGCTG TGTACGCCAC GTCAATGTCA CCACCTGTGA TGGAGCAGAT CATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT CCAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGGTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGGAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCA 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCGGCTC ATACCAAAGA AATACTTGAC 
ACGGCTTTGA AGGAAATAGA TGAAGTTGGG GATCTGCTGC AACTGAAGTA CTCCCGCCAC 
CGGCCGCTGC CTCTGCTGGA CAGGCCCTTT GACGAGACTA CCTATGAGGA GACAGAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptlc2 ( XM_008764823.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763045.2 Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61690
Clone ID Related Accession (Same CDS sequence)	XM_008764823.2
Accession Version	XM_008764823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine palmitoyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGG AGCCAGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTG 
AAAAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCAT CGCGGCTGCG 
GGCCAGATTC ATCATATTAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT AGTTGCTGTG CTCACATACG TGGGCTATGG TGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG GATCAGAGAC AACTGGAATC GACCCATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGACAG TCTCATGACT ACAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTTC TCAAGACATA TGGAGCAGGG 
GTGTGCAGCA CTCGCCAGGA AATTGGGAAC CTGGACAAGC ACGAGGAACT AGAGAAACTG 
GTAGCAAGGT TCTTGGGTGT GGAAGCTGCT TTGACCTATG GCATGGGATT TGCGACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCATCCT TGGTTCTAGG AGCCAGACTG TCGGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTGGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGGC CCTGGAAGAA AATCCTAATC CTCGTGGAAG GCATATATAG CATGGAAGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGTGGTGT AGTAGACTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATTGG CGGCAAGAAG GAGCTGATAG ACTACCTACG CACACATTCT 
CACAGTGCTG TGTACGCCAC GTCAATGTCA CCACCTGTGA TGGAGCAGAT CATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT CCAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGTTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGGAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCA 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCGGCTC ATACCAAAGA AATACCAGGA 
GAGAAAGCTT TGAAGGAGAT AGATGAAGTT GGGGATCTGC TGCAACTGAA GTACTCCCGC 
CACCGGCCGC TGCCTCTGCT GGACAGGCCC TTTGACGAGA CTACCTATGA GGAGACAGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763045.2
CDS	148..1833
Translation	MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATIAAAGQIHHITENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKTYGAGVCSTRQEIGNLDKHEELEKLVARFLGVEAALTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKECIQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEIPGEKALKEIDEVGDLLQLKYSRHRPLPLLDRPFDETTYEETED

Target ORF information:

RefSeq Version	XM_008764823.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764823.2

ORF Insert Sequence:

ATGCGGCCGG AGCCAGGAGG CTGCTGCTGC CGCCGCCCGA TGCGGGCGAA CGGCTGCGTG 
AAAAACGGGG AAGTGAGGAA CGGGTACTTG AGGAGCAGCA CCGCCACCAT CGCGGCTGCG 
GGCCAGATTC ATCATATTAC AGAAAATGGA GGACTGTACA AAAGACCGTT TAATGAAGCT 
TTTGAAGAAA CACCCATGCT AGTTGCTGTG CTCACATACG TGGGCTATGG TGTACTCACC 
CTCTTTGGAT ATCTTCGAGA TTTCTTGAGG CATTGGAGAA TTGAAAAGTG CCACCATGCA 
ACAGAAAGAG AAGAACAAAA GGACTTTGTG TCCTTGTATC AGGATTTTGA AAACTTCTAT 
ACAAGGAACC TCTACATGAG GATCAGAGAC AACTGGAATC GACCCATCTG TAGTGTGCCT 
GGAGCCAAGG TGGATATCAT GGAGAGACAG TCTCATGACT ACAACTGGTC ATTCAAGTAC 
ACAGGGAATA TAATTAAAGG TGTAATAAAC ATGGGTTCCT ACAACTATCT TGGATTTGCG 
AGGAACACTG GATCATGTCA GGAAGCAGCT GCTGAAGTTC TCAAGACATA TGGAGCAGGG 
GTGTGCAGCA CTCGCCAGGA AATTGGGAAC CTGGACAAGC ACGAGGAACT AGAGAAACTG 
GTAGCAAGGT TCTTGGGTGT GGAAGCTGCT TTGACCTATG GCATGGGATT TGCGACAAAT 
TCAATGAACA TTCCTGCTCT TGTTGGCAAA GGTTGCCTGA TTCTGAGTGA TGAGCTGAAC 
CATGCATCCT TGGTTCTAGG AGCCAGACTG TCGGGAGCAA CCATTCGAAT CTTCAAACAC 
AACAATATGC AAAGCTTGGA GAAGCTTTTA AAAGATGCCA TTGTTTATGG TCAGCCTCGG 
ACAAGAAGGC CCTGGAAGAA AATCCTAATC CTCGTGGAAG GCATATATAG CATGGAAGGG 
TCTATTGTTC GCCTTCCTGA AGTGATTGCT CTCAAGAAGA AATACAAGGC ATACTTGTAT 
CTGGATGAGG CTCACAGCAT TGGGGCTCTT GGCCCTTCAG GGCGTGGTGT AGTAGACTAC 
TTTGGCCTGG ATCCTGAGGA TGTAGATGTT ATGATGGGAA CATTCACAAA GAGCTTCGGT 
GCTTCAGGAG GATACATTGG CGGCAAGAAG GAGCTGATAG ACTACCTACG CACACATTCT 
CACAGTGCTG TGTACGCCAC GTCAATGTCA CCACCTGTGA TGGAGCAGAT CATCACCTCC 
ATGAAGTGCA TCATGGGGCA GGATGGCACC AGTCTTGGCA AAGAATGTAT CCAGCAGTTG 
GCTGAGAACA CCAGGTATTT CAGGAGACGC CTGAAGGAAA TGGGTTTCAT CATCTATGGC 
AATGAAGACT CCCCGGTGGT GCCTTTGATG CTCTACATGC CGGCCAAAAT TGGCGCCTTT 
GGGAGAGAGA TGCTGAAGCG GAACATTGGT GTAGTTGTGG TGGGATTTCC TGCTACCCCA 
ATCATTGAGT CCAGAGCCAG ATTTTGCCTG TCAGCGGCTC ATACCAAAGA AATACCAGGA 
GAGAAAGCTT TGAAGGAGAT AGATGAAGTT GGGGATCTGC TGCAACTGAA GTACTCCCGC 
CACCGGCCGC TGCCTCTGCT GGACAGGCCC TTTGACGAGA CTACCTATGA GGAGACAGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adipocyte-Specific Deficiency of De Novo Sphingolipid Biosynthesis Leads to Lipodystrophy and Insulin Resistance.
Diabetes66(10)2596-2609(2017 10)
Lee SY,Lee HY,Song JH,Kim GT,Jeon S,Song YJ,Lee JS,Hur JH,Oh HH,Park SY,Shim SM,Yoo HJ,Lee BC,Jiang XC,Choi CS,Park TS

Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation, and Metabolism.
Cell metabolism24(6)820-834(2016 Dec)
Chaurasia B,Kaddai VA,Lancaster GI,Henstridge DC,Sriram S,Galam DL,Gopalan V,Prakash KN,Velan SS,Bulchand S,Tsong TJ,Wang M,Siddique MM,Yuguang G,Sigmundsson K,Mellet NA,Weir JM,Meikle PJ,Bin M Yassin MS,Shabbir A,Shayman JA,Hirabayashi Y,Shiow ST,Sugii S,Summers SA

The Variant p.(Arg183Trp) in SPTLC2 Causes Late-Onset Hereditary Sensory Neuropathy.
Neuromolecular medicine18(1)81-90(2016 Mar)
Suriyanarayanan S,Auranen M,Toppila J,Paetau A,Shcherbii M,Palin E,Wei Y,Lohioja T,Schlotter-Weigel B,Sch?n U,Abicht A,Rautenstrauss B,Tyynismaa H,Walter MC,Hornemann T,Ylikallio E

Modulation of sphingolipid metabolism with calorie restriction enhances insulin action in skeletal muscle.
The Journal of nutritional biochemistry26(7)687-95(2015 Jul)
Obanda DN,Yu Y,Wang ZQ,Cefalu WT

ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.
Journal of lipid research56(4)898-908(2015 Apr)
Siow D,Sunkara M,Dunn TM,Morris AJ,Wattenberg B