Gaa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gaa
Entrez Gene ID	367562
Full Name	glucosidase, alpha, acid
General protein information	Preferred Names glucosidase, alpha, acid Names lysosomal alpha-glucosidase acid (Pompe disease, glycogen storage disease type II) acid alpha-glucosidase acid maltase glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	human homolog catalyzes the degradation of glycogen [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199118.1	NP_954549.1	lysosomal alpha-glucosidase precursor
XM_006247905.2	XP_006247967.1	lysosomal alpha-glucosidase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422082	XP_420085.4
Schizosaccharomyces pombe (fission yeast)	SPAC30D11.01c	NP_593216.2
Canis lupus familiaris (dog)	GAA	XP_005624079.1
Mus musculus (house mouse)	Gaa	NP_032090.3
Pan troglodytes (chimpanzee)	GAA	XP_001160653.1
Rattus norvegicus (Norway rat)	Gaa	NP_954549.1
Danio rerio (zebrafish)	gaa	XP_001921957.2
Schizosaccharomyces pombe (fission yeast)	agl1	NP_593996.1
Oryza sativa (rice)	Os01g0130400	NP_001041922.1
Bos taurus (cattle)	GAA	NP_776338.1
Schizosaccharomyces pombe (fission yeast)	SPAC1039.11c	XP_001713119.1
Neurospora crassa	NCU09281	XP_958500.1
Xenopus tropicalis (tropical clawed frog)	gaa	XP_002932561.2
Homo sapiens (human)	GAA	NP_001073272.1
	GAA	XP_001109980.1
Magnaporthe oryzae (rice blast fungus)	MGG_10662	XP_003710096.1
Arabidopsis thaliana (thale cress)	XYL1	NP_177023.1
Arabidopsis thaliana (thale cress)	AT3G45940	NP_190180.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Mortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)

Rat brain contains high levels of mannose-6-phosphorylated glycoproteins including lysosomal enzymes and palmitoyl-protein thioesterase, an enzyme implicated in infantile neuronal lipofuscinosis.
Sleat DE, Sohar I, Lackland H, Majercak J, Lobel P
The Journal of biological chemistry271(32)19191-8(1996 Aug)

Leaky splicing mutation in the acid maltase gene is associated with delayed onset of glycogenosis type II.
Boerkoel CF, Exelbert R, Nicastri C, Nichols RC, Miller FW, Plotz PH, Raben N
American journal of human genetics56(4)887-97(1995 Apr)

Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7.
Stallings RL, Siciliano MJ
Somatic cell genetics7(6)683-98(1981 Nov)

Simultaneous absence of alpha-1,4-glucosidase and alpha-1,6-glucosidase activities (pH 4) in tissues of children with type II glycogen storage disease.
Brown BI, Brown DH, Jeffrey PL
Biochemistry9(6)1423-8(1970 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05188	NM_199118.1 Latest version!	Rattus norvegicus glucosidase, alpha, acid (Gaa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa34043	XM_006247905.2 Latest version!	Rattus norvegicus glucosidase, alpha, acid (Gaa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gaa ( NM_199118.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954549.1 Rattus norvegicus glucosidase, alpha, acid (Gaa), mRNA.

Price & Availability↑

CloneID	ORa05188
Clone ID Related Accession (Same CDS sequence)	NM_199118.1
Accession Version	NM_199118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-glucosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061753.1. On Jan 1, 2004 this sequence version replaced XM_346213.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATATAC GGAAGCCCCT CTGTTCCAAC TCCGTGGTTG GGGCCTGCAC CCTCGTCTCC 
TTGACTACGG CTGTCATCCT GGGTCACCTC ATGCTCCGGG AGCTAATGCT ACTCCCCCAG 
GATCTTCATG AGTCCTCCTC GGGACTGTGG AAGACTTACC GACCTCACCA TCAGGAAAGT 
TACGAGCCAG CGCCCCTGCA CATCCAGGAG CACGCCGAAC AGCTAAGAGC AGTACCCACA 
CAGTGTGATG TAACCCCCAA CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCACCCAG 
GAGCAGTGCG AGGCCCGAGG CTGCTGCTGG GTCCCAGCAG GGCAGGTGCT GAATGGGCCA 
GTGATGGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG GCTAGAGAAC 
CTGAGCTCTA CAGAGTCAGG GTACACAGCC ACCCTGACCC GGACCAGTCC GACCTTCTTT 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACTGACAG CCGCCTCCAC 
TTTATGATCA AAGATCCCAC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCGCGTGTC 
CTGAGCCAGG CGCCATCCCC ACTTTACAGT GTGGAGTTCT CAGAGGAGCC CTTCGGCGTG 
ATCGTTCGCA GGAAGCTTGG TGGCCGAGTG CTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACC TCACTGCCCT CCCAGCACAT CGCAGGCCTG 
GGTGAGCACC TCAGTCCACT CATGCTCAGC ACCGAATGGA CTCGCATCAC CCTCTGGAAC 
CGGGATGTGG CACCCTCGCA AGGTGTCAAC CTCTATGGGT CACACCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ATAGCAATGC CATGGATGTG 
GTCCTGCAGC CCAGCCCGGC CCTAACTTGG AGGTCAACAG GCGGAATCCT GGATGTTTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGTGTT GTCCAACAAT ACCTGGATGT CGTGGGATAC 
CCCTTCATGC CTCCGTACTG GGGCCTGGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCC 
ACCGCCATCG TCCGACAGGT GGTGGAGAAC ATGACCAGGA CGCACTTCCC CCTGGACGTG 
CAGTGGAATG ACCTGGACTA CATGGACGCC CGGAGAGACT TCACTTTCAA CCAGGACGGC 
TTTGCAGACT TCCCAGACAT GGTGCACGAG CTCCACCAGG GTGGCCGGCG GTACATGATG 
ATTGTGGACC CTGCCATCAG CAGTTCAGGC CCTGCTGGGA GTTACAGGCC CTATGATGAG 
GGTCTTCGGA GGGGCGTGTT CATCACCAAT GAGACTGGGC AGCCATTGAT TGGGAAGGTT 
TGGCCTGGAT CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTCGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGATG GCATGTGGAT CGACATGAAC 
GAACCGTCCA ACTTCATCAG AGGCTCTCAG CAGGGCTGCC CTGACAATGA ACTAGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG GCCTTGCAGG CCGCCACCAT CTGTGCCTCC 
AGCCACCAGT TCCTCTCTAC ACACTACAAC CTCCACAACC TATACGGCCT CACTGAAGCA 
ATTGCCTCCA GCAGGGCCCT AGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTTG CGGGCCACGG CCGGTATGCC GGTCACTGGA CAGGGGACGT GTGGAGCTCT 
TGGGAGCATC TTGCGTACTC TGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CAGACATCTG CGGCTTCCAG GGAAACACGA CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCTTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCACAGCAGG CCATGAGGAA GGCCTTCACC 
TTGCGCTATG CCCTTCTACC CTACCTGTAT ACACTCTTCC ACGGCGCCCA CGTCAAAGGG 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTTATCACAC CTGTGCTTGA ACCTGGGAAA 
ACTGACGTGA CAGGCTACTT CCCCAAGGGC ATGTGGTACA ACCTGCAGAT GGTACCTGTG 
GAGACCCTTG GTAGCCTCCC TTCTTCGTCA CCAGCCTCGT CTTTCAGATC TATTGTCCAC 
AGCAAGGGTC AGTGGCTGAC ACTGGAAGCC CCCCTGGATA CCATCAACGT GCACCTCAGG 
GCGGGGTACA TCATACCACT GCAGGGTCCC AGCCTCACAA CCACAGAGTC CCGAAAGCAG 
CCCATGGCCC TGGCTGTGGC GTTAACTGAA AGCGGCGAGG CCTCTGGGGA GCTGTTTTGG 
GACGACGGGG AGAGCCTCGG GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAATA ATACCATTGT AAACAAGTTA GTGCACGTGA CCAAGGAGGG AGGTGAACTG 
CAACTGAGGG AGGTGACCAT CTTGGGAGTG ACCACAGCTC CCACCCAGGT CCTTTCCAAT 
GGCATCTCTG TCTCCAATTT CACCTACAGC CCGGACGACA AGAGCCTGTC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCG GTTTCAAATC GATTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954549.1
CDS	171..3032
Translation	MNIRKPLCSNSVVGACTLVSLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQESYEPAPLHIQEHAEQLRAVPTQCDVTPNSRFDCAPDKGITQEQCEARGCCWVPAGQVLNGPVMGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFMIKDPTSKRYEVPLETPRVLSQAPSPLYSVEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHIAGLGEHLSPLMLSTEWTRITLWNRDVAPSQGVNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVVLQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDGFADFPDMVHELHQGGRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWIDMNEPSNFIRGSQQGCPDNELENPPYVPGVVGGALQAATICASSHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEHLAYSVPEILQFNLLGVPLVGADICGFQGNTTEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSETAQQAMRKAFTLRYALLPYLYTLFHGAHVKGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTDVTGYFPKGMWYNLQMVPVETLGSLPSSSPASSFRSIVHSKGQWLTLEAPLDTINVHLRAGYIIPLQGPSLTTTESRKQPMALAVALTESGEASGELFWDDGESLGVLERGAYTLVTFSAKNNTIVNKLVHVTKEGGELQLREVTILGVTTAPTQVLSNGISVSNFTYSPDDKSLSIPVSLLMGERFQIDWS

Target ORF information:

RefSeq Version	NM_199118.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosidase, alpha, acid (Gaa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199118.1

ORF Insert Sequence:

ATGAATATAC GGAAGCCCCT CTGTTCCAAC TCCGTGGTTG GGGCCTGCAC CCTCGTCTCC 
TTGACTACGG CTGTCATCCT GGGTCACCTC ATGCTCCGGG AGCTAATGCT ACTCCCCCAG 
GATCTTCATG AGTCCTCCTC GGGACTGTGG AAGACTTACC GACCTCACCA TCAGGAAAGT 
TACGAGCCAG CGCCCCTGCA CATCCAGGAG CACGCCGAAC AGCTAAGAGC AGTACCCACA 
CAGTGTGATG TAACCCCCAA CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCACCCAG 
GAGCAGTGCG AGGCCCGAGG CTGCTGCTGG GTCCCAGCAG GGCAGGTGCT GAATGGGCCA 
GTGATGGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG GCTAGAGAAC 
CTGAGCTCTA CAGAGTCAGG GTACACAGCC ACCCTGACCC GGACCAGTCC GACCTTCTTT 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACTGACAG CCGCCTCCAC 
TTTATGATCA AAGATCCCAC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCGCGTGTC 
CTGAGCCAGG CGCCATCCCC ACTTTACAGT GTGGAGTTCT CAGAGGAGCC CTTCGGCGTG 
ATCGTTCGCA GGAAGCTTGG TGGCCGAGTG CTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACC TCACTGCCCT CCCAGCACAT CGCAGGCCTG 
GGTGAGCACC TCAGTCCACT CATGCTCAGC ACCGAATGGA CTCGCATCAC CCTCTGGAAC 
CGGGATGTGG CACCCTCGCA AGGTGTCAAC CTCTATGGGT CACACCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ATAGCAATGC CATGGATGTG 
GTCCTGCAGC CCAGCCCGGC CCTAACTTGG AGGTCAACAG GCGGAATCCT GGATGTTTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGTGTT GTCCAACAAT ACCTGGATGT CGTGGGATAC 
CCCTTCATGC CTCCGTACTG GGGCCTGGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCC 
ACCGCCATCG TCCGACAGGT GGTGGAGAAC ATGACCAGGA CGCACTTCCC CCTGGACGTG 
CAGTGGAATG ACCTGGACTA CATGGACGCC CGGAGAGACT TCACTTTCAA CCAGGACGGC 
TTTGCAGACT TCCCAGACAT GGTGCACGAG CTCCACCAGG GTGGCCGGCG GTACATGATG 
ATTGTGGACC CTGCCATCAG CAGTTCAGGC CCTGCTGGGA GTTACAGGCC CTATGATGAG 
GGTCTTCGGA GGGGCGTGTT CATCACCAAT GAGACTGGGC AGCCATTGAT TGGGAAGGTT 
TGGCCTGGAT CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTCGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGATG GCATGTGGAT CGACATGAAC 
GAACCGTCCA ACTTCATCAG AGGCTCTCAG CAGGGCTGCC CTGACAATGA ACTAGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG GCCTTGCAGG CCGCCACCAT CTGTGCCTCC 
AGCCACCAGT TCCTCTCTAC ACACTACAAC CTCCACAACC TATACGGCCT CACTGAAGCA 
ATTGCCTCCA GCAGGGCCCT AGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTTG CGGGCCACGG CCGGTATGCC GGTCACTGGA CAGGGGACGT GTGGAGCTCT 
TGGGAGCATC TTGCGTACTC TGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CAGACATCTG CGGCTTCCAG GGAAACACGA CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCTTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCACAGCAGG CCATGAGGAA GGCCTTCACC 
TTGCGCTATG CCCTTCTACC CTACCTGTAT ACACTCTTCC ACGGCGCCCA CGTCAAAGGG 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTTATCACAC CTGTGCTTGA ACCTGGGAAA 
ACTGACGTGA CAGGCTACTT CCCCAAGGGC ATGTGGTACA ACCTGCAGAT GGTACCTGTG 
GAGACCCTTG GTAGCCTCCC TTCTTCGTCA CCAGCCTCGT CTTTCAGATC TATTGTCCAC 
AGCAAGGGTC AGTGGCTGAC ACTGGAAGCC CCCCTGGATA CCATCAACGT GCACCTCAGG 
GCGGGGTACA TCATACCACT GCAGGGTCCC AGCCTCACAA CCACAGAGTC CCGAAAGCAG 
CCCATGGCCC TGGCTGTGGC GTTAACTGAA AGCGGCGAGG CCTCTGGGGA GCTGTTTTGG 
GACGACGGGG AGAGCCTCGG GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAATA ATACCATTGT AAACAAGTTA GTGCACGTGA CCAAGGAGGG AGGTGAACTG 
CAACTGAGGG AGGTGACCAT CTTGGGAGTG ACCACAGCTC CCACCCAGGT CCTTTCCAAT 
GGCATCTCTG TCTCCAATTT CACCTACAGC CCGGACGACA AGAGCCTGTC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCG GTTTCAAATC GATTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gaa ( XM_006247905.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247967.1 Rattus norvegicus glucosidase, alpha, acid (Gaa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34043
Clone ID Related Accession (Same CDS sequence)	XM_006247905.2
Accession Version	XM_006247905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAC GGAAGCCCCT CTGTTCCAAC TCCGTGGTTG GGGCCTGCAC CCTCGTCTCC 
TTGACTACGG CTGTCATCCT GGGTCACCTC ATGCTCCGGG AGCTAATGCT ACTCCCCCAG 
GATCTTCATG AGTCCTCCTC GGGACTGTGG AAGACTTACC GACCTCACCA TCAGGAAAGT 
TACGAGCCAG CGCCCCTGCA CATCCAGGAG CACGCCGAAC AGCTAAGAGC AGTACCCACA 
CAGTGTGATG TAACCCCCAA CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCACCCAG 
GAGCAGTGCG AGGCCCGAGG CTGCTGCTGG GTCCCAGCAG GGCAGGTGCT GAATGGGCCA 
GTGATGGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG GCTAGAGAAC 
CTGAGCTCTA CAGAGTCAGG GTACACAGCC ACCCTGACCC GGACCAGTCC GACCTTCTTT 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACTGACAG CCGCCTCCAC 
TTTATGATCA AAGATCCCAC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCGCGTGTC 
CTGAGCCAGG CGCCATCCCC ACTTTACAGT GTGGAGTTCT CAGAGGAGCC CTTTGGAGTG 
ATCGTTCGCA GGAAGCTTGG TGGCCGAGTG CTGCTGAACA CAACTGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACC TCACTGCCCT CCCAGCACAT CACAGGCCTG 
GGTGAGCACC TCAGTCCACT CATGCTCAGC ACCGAATGGA CTCGCATCAC CCTCTGGAAC 
CGGGATGTGG CACCCTCGCA AGGTGTCAAC CTCTATGGGT CACACCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ATAGCAATGC CATGGATGTG 
GTCCTGCAGC CCAGCCCGGC CCTAACTTGG AGGTCAACAG GCGGAATCCT GGATGTTTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGTGTT GTCCAACAAT ACCTGGATGT CGTGGGATAC 
CCCTTCATGC CTCCGTACTG GGGCCTGGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCC 
ACCGCCATCG TCCGACAGGT GGTGGAGAAC ATGACCAGGA CGCACTTCCC CCTGGACGTG 
CAGTGGAATG ACCTGGACTA CATGGACGCC CGGAGAGACT TCACTTTCAA CCAGGACGGC 
TTTGCAGACT TCCCAGACAT GGTGCACGAG CTCCACCAGG GTGGCCGGCG GTACATGATG 
ATTGTGGACC CTGCCATCAG CAGTTCAGGC CCTGCTGGGA GTTACAGGCC CTATGATGAG 
GGTCTTCGGA GGGGCGTGTT CATCACCAAT GAGACCGGGC AGCCATTGAT TGGGAAGGTT 
TGGCCTGGAT CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTCGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGATG GCATGTGGAT CGACATGAAC 
GAACCGTCCA ACTTCATCAG AGGCTCTCAG CAGGGCTGCC CTGACAATGA ACTAGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG GCCTTGCAGG CCGCCACCAT CTGTGCCTCC 
AGCCACCAGT TCCTCTCTAC ACACTACAAC CTCCACAACC TATACGGCCT CACTGAAGCG 
ATTGCCTCCA GCAGGGCCCT AGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTTG CGGGCCACGG CCTGTATGCC GGTCACTGGA CAGGGGACGT GTGGAGCTCT 
TGGGAGCATC TTGCGTACTC TGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CAGACATCTG CGGCTTCCAG GGAAACACGA CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCTTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCACAGCAGG CCATGAGGAA GGCCTTCACC 
TTGCGCTATG CCCTTCTACC CTACCTGTAT ACACTCTTCC ACGGCGCCCA CGTCAAAGGG 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTTGTCACAC CTGTGCTTGA ACCTGGGAAA 
ACTGACGTGA CAGGCTACTT CCCCAAGGGC ATGTGGTACA ACCTGCAGAT GGTACCTGTG 
GAGACCCTTG GTAGCCTCCC TTCTTCGTCA CCAGCCTCGT CTTTCAGATC TATTGTCCAC 
AGCAAGGGTC AGTGGCTGAC ACTGGAAGCC CCCCTGGATA CCATCAACGT GCACCTCAGG 
GCGGGGTACA TCATACCACT GCAGGGTCCC AGCCTCACAA CCACAGAGTC CCGAAAGCAG 
CCCATGGCCC TGGCTGTGGC GTTAACTGAA AGCGGCGAGG CCTCTGGGGA GCTGTTTTGG 
GACGACGGGG AGAGCCTCGG GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAATA ATACCATTGT AAACAAGTTA GTGCACGTGA CCAAGGAGGG AGGTGAACTG 
CAACTGAGGG AGGTGACCAT CTTGGGAGTG ACCACAGCTC CCACCCAGGT CCTTTCCAAT 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCGGACGACA AGACCCTGTC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCG GTTTCAAATC GATTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247967.1
CDS	152..3013
Translation	MNIRKPLCSNSVVGACTLVSLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQESYEPAPLHIQEHAEQLRAVPTQCDVTPNSRFDCAPDKGITQEQCEARGCCWVPAGQVLNGPVMGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFMIKDPTSKRYEVPLETPRVLSQAPSPLYSVEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLMLSTEWTRITLWNRDVAPSQGVNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVVLQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDGFADFPDMVHELHQGGRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWIDMNEPSNFIRGSQQGCPDNELENPPYVPGVVGGALQAATICASSHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFAGHGLYAGHWTGDVWSSWEHLAYSVPEILQFNLLGVPLVGADICGFQGNTTEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSETAQQAMRKAFTLRYALLPYLYTLFHGAHVKGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLVTPVLEPGKTDVTGYFPKGMWYNLQMVPVETLGSLPSSSPASSFRSIVHSKGQWLTLEAPLDTINVHLRAGYIIPLQGPSLTTTESRKQPMALAVALTESGEASGELFWDDGESLGVLERGAYTLVTFSAKNNTIVNKLVHVTKEGGELQLREVTILGVTTAPTQVLSNGIPVSNFTYSPDDKTLSIPVSLLMGERFQIDWS

Target ORF information:

RefSeq Version	XM_006247905.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosidase, alpha, acid (Gaa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247905.2

ORF Insert Sequence:

ATGAATATAC GGAAGCCCCT CTGTTCCAAC TCCGTGGTTG GGGCCTGCAC CCTCGTCTCC 
TTGACTACGG CTGTCATCCT GGGTCACCTC ATGCTCCGGG AGCTAATGCT ACTCCCCCAG 
GATCTTCATG AGTCCTCCTC GGGACTGTGG AAGACTTACC GACCTCACCA TCAGGAAAGT 
TACGAGCCAG CGCCCCTGCA CATCCAGGAG CACGCCGAAC AGCTAAGAGC AGTACCCACA 
CAGTGTGATG TAACCCCCAA CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCACCCAG 
GAGCAGTGCG AGGCCCGAGG CTGCTGCTGG GTCCCAGCAG GGCAGGTGCT GAATGGGCCA 
GTGATGGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG GCTAGAGAAC 
CTGAGCTCTA CAGAGTCAGG GTACACAGCC ACCCTGACCC GGACCAGTCC GACCTTCTTT 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACTGACAG CCGCCTCCAC 
TTTATGATCA AAGATCCCAC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCGCGTGTC 
CTGAGCCAGG CGCCATCCCC ACTTTACAGT GTGGAGTTCT CAGAGGAGCC CTTTGGAGTG 
ATCGTTCGCA GGAAGCTTGG TGGCCGAGTG CTGCTGAACA CAACTGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACC TCACTGCCCT CCCAGCACAT CACAGGCCTG 
GGTGAGCACC TCAGTCCACT CATGCTCAGC ACCGAATGGA CTCGCATCAC CCTCTGGAAC 
CGGGATGTGG CACCCTCGCA AGGTGTCAAC CTCTATGGGT CACACCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ATAGCAATGC CATGGATGTG 
GTCCTGCAGC CCAGCCCGGC CCTAACTTGG AGGTCAACAG GCGGAATCCT GGATGTTTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGTGTT GTCCAACAAT ACCTGGATGT CGTGGGATAC 
CCCTTCATGC CTCCGTACTG GGGCCTGGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCC 
ACCGCCATCG TCCGACAGGT GGTGGAGAAC ATGACCAGGA CGCACTTCCC CCTGGACGTG 
CAGTGGAATG ACCTGGACTA CATGGACGCC CGGAGAGACT TCACTTTCAA CCAGGACGGC 
TTTGCAGACT TCCCAGACAT GGTGCACGAG CTCCACCAGG GTGGCCGGCG GTACATGATG 
ATTGTGGACC CTGCCATCAG CAGTTCAGGC CCTGCTGGGA GTTACAGGCC CTATGATGAG 
GGTCTTCGGA GGGGCGTGTT CATCACCAAT GAGACCGGGC AGCCATTGAT TGGGAAGGTT 
TGGCCTGGAT CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTCGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGATG GCATGTGGAT CGACATGAAC 
GAACCGTCCA ACTTCATCAG AGGCTCTCAG CAGGGCTGCC CTGACAATGA ACTAGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG GCCTTGCAGG CCGCCACCAT CTGTGCCTCC 
AGCCACCAGT TCCTCTCTAC ACACTACAAC CTCCACAACC TATACGGCCT CACTGAAGCG 
ATTGCCTCCA GCAGGGCCCT AGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTTG CGGGCCACGG CCTGTATGCC GGTCACTGGA CAGGGGACGT GTGGAGCTCT 
TGGGAGCATC TTGCGTACTC TGTGCCAGAA ATCCTGCAGT TTAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CAGACATCTG CGGCTTCCAG GGAAACACGA CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCTTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCACAGCAGG CCATGAGGAA GGCCTTCACC 
TTGCGCTATG CCCTTCTACC CTACCTGTAT ACACTCTTCC ACGGCGCCCA CGTCAAAGGG 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTTGTCACAC CTGTGCTTGA ACCTGGGAAA 
ACTGACGTGA CAGGCTACTT CCCCAAGGGC ATGTGGTACA ACCTGCAGAT GGTACCTGTG 
GAGACCCTTG GTAGCCTCCC TTCTTCGTCA CCAGCCTCGT CTTTCAGATC TATTGTCCAC 
AGCAAGGGTC AGTGGCTGAC ACTGGAAGCC CCCCTGGATA CCATCAACGT GCACCTCAGG 
GCGGGGTACA TCATACCACT GCAGGGTCCC AGCCTCACAA CCACAGAGTC CCGAAAGCAG 
CCCATGGCCC TGGCTGTGGC GTTAACTGAA AGCGGCGAGG CCTCTGGGGA GCTGTTTTGG 
GACGACGGGG AGAGCCTCGG GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAATA ATACCATTGT AAACAAGTTA GTGCACGTGA CCAAGGAGGG AGGTGAACTG 
CAACTGAGGG AGGTGACCAT CTTGGGAGTG ACCACAGCTC CCACCCAGGT CCTTTCCAAT 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCGGACGACA AGACCCTGTC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCG GTTTCAAATC GATTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)
Mortensen M,Ferguson DJ,Edelmann M,Kessler B,Morten KJ,Komatsu M,Simon AK

Rat brain contains high levels of mannose-6-phosphorylated glycoproteins including lysosomal enzymes and palmitoyl-protein thioesterase, an enzyme implicated in infantile neuronal lipofuscinosis.
The Journal of biological chemistry271(32)19191-8(1996 Aug)
Sleat DE,Sohar I,Lackland H,Majercak J,Lobel P

Leaky splicing mutation in the acid maltase gene is associated with delayed onset of glycogenosis type II.
American journal of human genetics56(4)887-97(1995 Apr)
Boerkoel CF,Exelbert R,Nicastri C,Nichols RC,Miller FW,Plotz PH,Raben N

Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7.
Somatic cell genetics7(6)683-98(1981 Nov)
Stallings RL,Siciliano MJ

Simultaneous absence of alpha-1,4-glucosidase and alpha-1,6-glucosidase activities (pH 4) in tissues of children with type II glycogen storage disease.
Biochemistry9(6)1423-8(1970 Mar)
Brown BI,Brown DH,Jeffrey PL