Stk25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stk25
Entrez Gene ID	373542
Full Name	serine/threonine kinase 25
General protein information	Preferred Names serine/threonine kinase 25 Names serine/threonine-protein kinase 25 serine/threonine kinase 25 (STE20 homolog, yeast)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q36
Summary	human homolog is a member of the Ste20-like kinase family which is activated by cellular, particularly oxidant, stress but which does not activate either of the stress-activated MAP kinase cascades (p38 and SAPKs) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_184049.1	NP_908938.1	serine/threonine-protein kinase 25

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STK25	XP_005636037.1
Danio rerio (zebrafish)	stk25b	NP_998473.1
Pan troglodytes (chimpanzee)	STK25	XP_001162228.2
Bos taurus (cattle)	STK25	NP_899666.1
Rattus norvegicus (Norway rat)	Stk25	NP_908938.1
Caenorhabditis elegans (roundworm)	gck-1	NP_001024141.1
Xenopus tropicalis (tropical clawed frog)	stk25	NP_001015684.1
Homo sapiens (human)	STK25	NP_006365.2
Mus musculus (house mouse)	Stk25	NP_067512.3
	STK25	XP_001092650.1
Gallus gallus (chicken)	STK25	NP_989705.1

Gene Ontology
Bibliography

Related articles in PubMed

Serine/threonine protein kinase 25 (STK25): a novel negative regulator of lipid and glucose metabolism in rodent and human skeletal muscle.
Nerstedt A, Cansby E, Andersson CX, Laakso M, Stan??kov? A, Bl?her M, Smith U, Mahlapuu M
Diabetologia55(6)1797-807(2012 Jun)

PDCD10 interacts with STK25 to accelerate cell apoptosis under oxidative stress.
Zhang H, Ma X, Deng X, Chen Y, Mo X, Zhang Y, Zhao H, Ma D
Frontiers in bioscience (Landmark edition)172295-305(2012 Jun)

Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress.
Fidalgo M, Guerrero A, Fraile M, Iglesias C, Pombo CM, Zalvide J
The Journal of biological chemistry287(14)11556-65(2012 Mar)

Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment.
Matsuki T, Matthews RT, Cooper JA, van der Brug MP, Cookson MR, Hardy JA, Olson EC, Howell BW
Cell143(5)826-36(2010 Nov)

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.
Fidalgo M, Fraile M, Pires A, Force T, Pombo C, Zalvide J
Journal of cell science123(Pt 8)1274-84(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study indicates a possible role for STK25 in the regulation of glucose and lipid metabolism in L6 myoblasts.
Title: Serine/threonine protein kinase 25 (STK25): a novel negative regulator of lipid and glucose metabolism in rodent and human skeletal muscle.

The following Stk25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stk25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04721	NM_184049.1 Latest version!	Rattus norvegicus serine/threonine kinase 25 (Stk25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stk25 ( NM_184049.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_908938.1 Rattus norvegicus serine/threonine kinase 25 (Stk25), mRNA.

Price & Availability↑

CloneID	ORa04721
Clone ID Related Accession (Same CDS sequence)	NM_184049.1
Accession Version	NM_184049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY346152.1. On Jan 12, 2005 this sequence version replaced XM_343632.1. ##Evidence-Data-START## Transcript exon combination :: AY346152.1, BC087092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCACC TCAGGGGCTT CGCGCACCAG CATTCTCGTG TGGACCCAGA GGAACTCTTC 
ACCAAGCTTG ACCGAATTGG CAAGGGCTCA TTTGGGGAGG TGTACAAGGG GATCGACAAC 
CACACGAAGG AGGTGGTGGC CATCAAGATC ATTGACCTGG AAGAGGCCGA GGATGAGATT 
GAGGACATCC AACAGGAGAT CACCGTGCTC AGCCAATGTG ATAGCCCTTA CATCACTCGC 
TACTTCGGCT CCTACCTAAA GAGCACCAAG CTATGGATTA TCATGGAGTA CCTGGGTGGT 
GGCTCTGCAC TGGACTTGCT GAAACCTGGT CCACTGGAGG AGACCTATAT TGCCACCATC 
TTGCGGGAGA TCCTGAAAGG CTTGGATTAT CTGCATTCAG AGCGCAAGAT CCACCGAGAC 
ATCAAAGCTG CCAACGTGCT GCTCTCAGAG CAGGGTGATG TGAAGCTGGC AGACTTCGGT 
GTGGCTGGGC AGCTCACAGA CACACAAATC AAGAGGAACA CATTTGTGGG TACTCCCTTC 
TGGATGGCAC CTGAGGTTAT CAAGCAGTCG GCCTATGACT TCAAGGCTGA CATCTGGTCC 
CTGGGCATCA CAGCCATCGA GCTGGCCAAG GGGGAGCCGC CAAACTCTGA CCTCCACCCT 
ATGCGAGTCC TGTTCCTGAT TCCCAAGAAC AATCCACCCA CATTGGAAGG CCACCACAGC 
AAACCCTTCA AGGAGTTTGT AGAGGCGTGC CTCAATAAGG ACCCACGATT CCGACCCACT 
GCCAAGGAGC TCCTGAAGCA CAAATTCATC ACACGTTACA CCAAGAAGAC CTCCTTTCTC 
ACTGAACTTA TTGACCGATA TAAGCGTTGG AAGTCTGAGG GGCATGGCGA GGAGTCCAGT 
TCTGAGGATT CTGACATTGA TGGCGAGGCT GAGGATGGGG AGCAGGGTCC CATCTGGACG 
TTTCCTCCAA CCATCCGGCC AAGTCCACAT AGCAAGCTGC ACAAGGGGAC TGCTCTGCAC 
AGCTCACAGA AGCCTGCTGA GCCTATCAAG AGACAGCCAA GGTCCCAGTG CTTGTCCACA 
CTAGTCCGTC CTGTCTTTGG AGAGCTCAAA GAGAAGCACA AGCAGAGCGG TGGGAGCGTG 
GGTGCCCTGG AGGAGCTAGA GAATGCCTTC AGCCTGGCTG AGGAATCCTG TCCTGGCATC 
TCAGACAAGC TGATGGTGCA TCTGGTGGAG CGTGTGCAGA GGTTCTCACA CAGCAGAAAC 
CACCTGACAT CTACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_908938.1
CDS	162..1442
Translation	MAHLRGFAHQHSRVDPEELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGHHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTSFLTELIDRYKRWKSEGHGEESSSEDSDIDGEAEDGEQGPIWTFPPTIRPSPHSKLHKGTALHSSQKPAEPIKRQPRSQCLSTLVRPVFGELKEKHKQSGGSVGALEELENAFSLAEESCPGISDKLMVHLVERVQRFSHSRNHLTSTR

Target ORF information:

RefSeq Version	NM_184049.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine/threonine kinase 25 (Stk25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_184049.1

ORF Insert Sequence:

ATGGCTCACC TCAGGGGCTT CGCGCACCAG CATTCTCGTG TGGACCCAGA GGAACTCTTC 
ACCAAGCTTG ACCGAATTGG CAAGGGCTCA TTTGGGGAGG TGTACAAGGG GATCGACAAC 
CACACGAAGG AGGTGGTGGC CATCAAGATC ATTGACCTGG AAGAGGCCGA GGATGAGATT 
GAGGACATCC AACAGGAGAT CACCGTGCTC AGCCAATGTG ATAGCCCTTA CATCACTCGC 
TACTTCGGCT CCTACCTAAA GAGCACCAAG CTATGGATTA TCATGGAGTA CCTGGGTGGT 
GGCTCTGCAC TGGACTTGCT GAAACCTGGT CCACTGGAGG AGACCTATAT TGCCACCATC 
TTGCGGGAGA TCCTGAAAGG CTTGGATTAT CTGCATTCAG AGCGCAAGAT CCACCGAGAC 
ATCAAAGCTG CCAACGTGCT GCTCTCAGAG CAGGGTGATG TGAAGCTGGC AGACTTCGGT 
GTGGCTGGGC AGCTCACAGA CACACAAATC AAGAGGAACA CATTTGTGGG TACTCCCTTC 
TGGATGGCAC CTGAGGTTAT CAAGCAGTCG GCCTATGACT TCAAGGCTGA CATCTGGTCC 
CTGGGCATCA CAGCCATCGA GCTGGCCAAG GGGGAGCCGC CAAACTCTGA CCTCCACCCT 
ATGCGAGTCC TGTTCCTGAT TCCCAAGAAC AATCCACCCA CATTGGAAGG CCACCACAGC 
AAACCCTTCA AGGAGTTTGT AGAGGCGTGC CTCAATAAGG ACCCACGATT CCGACCCACT 
GCCAAGGAGC TCCTGAAGCA CAAATTCATC ACACGTTACA CCAAGAAGAC CTCCTTTCTC 
ACTGAACTTA TTGACCGATA TAAGCGTTGG AAGTCTGAGG GGCATGGCGA GGAGTCCAGT 
TCTGAGGATT CTGACATTGA TGGCGAGGCT GAGGATGGGG AGCAGGGTCC CATCTGGACG 
TTTCCTCCAA CCATCCGGCC AAGTCCACAT AGCAAGCTGC ACAAGGGGAC TGCTCTGCAC 
AGCTCACAGA AGCCTGCTGA GCCTATCAAG AGACAGCCAA GGTCCCAGTG CTTGTCCACA 
CTAGTCCGTC CTGTCTTTGG AGAGCTCAAA GAGAAGCACA AGCAGAGCGG TGGGAGCGTG 
GGTGCCCTGG AGGAGCTAGA GAATGCCTTC AGCCTGGCTG AGGAATCCTG TCCTGGCATC 
TCAGACAAGC TGATGGTGCA TCTGGTGGAG CGTGTGCAGA GGTTCTCACA CAGCAGAAAC 
CACCTGACAT CTACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Serine/threonine protein kinase 25 (STK25): a novel negative regulator of lipid and glucose metabolism in rodent and human skeletal muscle.
Diabetologia55(6)1797-807(2012 Jun)
Nerstedt A,Cansby E,Andersson CX,Laakso M,Stan??kov? A,Bl?her M,Smith U,Mahlapuu M

PDCD10 interacts with STK25 to accelerate cell apoptosis under oxidative stress.
Frontiers in bioscience (Landmark edition)172295-305(2012 Jun)
Zhang H,Ma X,Deng X,Chen Y,Mo X,Zhang Y,Zhao H,Ma D

Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress.
The Journal of biological chemistry287(14)11556-65(2012 Mar)
Fidalgo M,Guerrero A,Fraile M,Iglesias C,Pombo CM,Zalvide J

Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment.
Cell143(5)826-36(2010 Nov)
Matsuki T,Matthews RT,Cooper JA,van der Brug MP,Cookson MR,Hardy JA,Olson EC,Howell BW

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.
Journal of cell science123(Pt 8)1274-84(2010 Apr)
Fidalgo M,Fraile M,Pires A,Force T,Pombo C,Zalvide J

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Stk25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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