Mis12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Mis12
Entrez Gene ID	501706
Full Name	MIS12 kinetochore complex component
General protein information	Preferred Names MIS12 kinetochore complex component Names protein MIS12 homolog MIS12, MIND kinetochore complex component, homolog liver regeneration-related protein LRRG112
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246793.3	XP_006246855.1	protein MIS12 homolog isoform X1
XM_017597477.1	XP_017452966.1	protein MIS12 homolog isoform X1
XM_006246791.3	XP_006246853.1	protein MIS12 homolog isoform X1
XM_006246794.3	XP_006246856.1	protein MIS12 homolog isoform X1
NM_001047972.1	NP_001041437.1	protein MIS12 homolog

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MIS12	XP_005619942.1
Rattus norvegicus (Norway rat)	Mis12	NP_001041437.1
Homo sapiens (human)	MIS12	NP_001245149.1
Pan troglodytes (chimpanzee)	MIS12	XP_003953003.1
Danio rerio (zebrafish)	zgc:64116	NP_956672.1
	MIS12	XP_001101672.1
Canis lupus familiaris (dog)	LOC102154136	XP_005620242.1
Bos taurus (cattle)	MIS12	NP_001070437.1
Mus musculus (house mouse)	Mis12	NP_080269.1
Gallus gallus (chicken)	MIS12	NP_001038142.1
Xenopus tropicalis (tropical clawed frog)	mis12	NP_001016808.1

Gene Ontology
Bibliography

Related articles in PubMed

Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Shrestha RL, Draviam VM
Current biology : CB23(16)1514-26(2013 Aug)

The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation.
Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A
The Journal of cell biology173(1)9-17(2006 Apr)

A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.
Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR 3rd, Oegema K, Desai A
Genes & development18(18)2255-68(2004 Sep)

Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway.
Goshima G, Kiyomitsu T, Yoda K, Yanagida M
The Journal of cell biology160(1)25-39(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mis12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mis12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42284	NM_001047972.1 Latest version!	Rattus norvegicus MIS12 kinetochore complex component (Mis12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa33372	XM_006246791.3 Latest version!	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa33372	XM_006246793.3 Latest version!	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa33372	XM_006246794.3 Latest version!	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa33372	XM_017597477.1 Latest version!	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mis12 ( NM_001047972.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001041437.1 Rattus norvegicus MIS12 kinetochore complex component (Mis12), mRNA.

Price & Availability↑

CloneID	ORa42284
Clone ID Related Accession (Same CDS sequence)	NM_001047972.1
Accession Version	NM_001047972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Rattus norvegicus(Norway rat)
Product	protein MIS12 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY310159.2. On or before Sep 8, 2006 this sequence version replaced XM_577111.2, XM_001080081.1. ##Evidence-Data-START## Transcript exon combination :: AY310159.2 [ECO:0000332] ##Evidence-Data-END##

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGGGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041437.1
CDS	41..661
Translation	MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVILKKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPEDKCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKEGKRLETQ

Target ORF information:

RefSeq Version	NM_001047972.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MIS12 kinetochore complex component (Mis12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047972.1

ORF Insert Sequence:

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGGGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mis12 ( XM_006246793.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246855.1 Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33372
Clone ID Related Accession (Same CDS sequence)	XM_006246793.3 , XM_017597477.1 , XM_006246791.3 , XM_006246794.3
Accession Version	XM_006246793.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein MIS12 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246793.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246855.1
CDS	153..773
Translation	MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVILKKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPEDKCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKESKRLETQ

Target ORF information:

RefSeq Version	XM_006246793.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246793.3

ORF Insert Sequence:

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mis12 ( XM_017597477.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452966.1 Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33372
Clone ID Related Accession (Same CDS sequence)	XM_006246793.3 , XM_017597477.1 , XM_006246791.3 , XM_006246794.3
Accession Version	XM_017597477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein MIS12 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452966.1
CDS	314..934
Translation	MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVILKKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPEDKCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKESKRLETQ

Target ORF information:

RefSeq Version	XM_017597477.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597477.1

ORF Insert Sequence:

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mis12 ( XM_006246791.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246853.1 Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33372
Clone ID Related Accession (Same CDS sequence)	XM_006246793.3 , XM_017597477.1 , XM_006246791.3 , XM_006246794.3
Accession Version	XM_006246791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein MIS12 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246853.1
CDS	431..1051
Translation	MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVILKKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPEDKCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKESKRLETQ

Target ORF information:

RefSeq Version	XM_006246791.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246791.3

ORF Insert Sequence:

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mis12 ( XM_006246794.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246856.1 Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33372
Clone ID Related Accession (Same CDS sequence)	XM_006246793.3 , XM_017597477.1 , XM_006246791.3 , XM_006246794.3
Accession Version	XM_006246794.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein MIS12 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246856.1
CDS	269..889
Translation	MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVILKKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPEDKCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKESKRLETQ

Target ORF information:

RefSeq Version	XM_006246794.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MIS12 kinetochore complex component (Mis12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246794.3

ORF Insert Sequence:

ATGTCTGTGG ATCCGATGGC CTATGAGGCC CAGTTCTTTG GCTTCACCCC ACAGACTTGC 
TTGCTGAGGA TCTACATAGC ATTTCAAGAC CACCTCTTTG AAGTGATGCA GGCTGTTGAA 
CAGGTTATCT TAAAGAAGCT GGAGGGCATT CCAGACTGTG AGATTAGCTC TATCCAGACT 
CGTAAATGCA CAGAGAAGTT TCTTTGCTTC ATGAAAGGAC GTTTTGATAA CCTTTTTGGT 
AAAATGGAGC AGCTGATTTT ACAATCGATT TTGCAAATTC CTCCAAACAT CCTGCTTCCT 
GAAGATAAGT GTCAGGAGAC GAATCCTTTC AGTGAAGAAA AATTCCAGCT TCTCAAACAG 
GAAATCAAAG AGTTACAGGA GAAATATAAG GTTGAATTAT GCACTGAGCA GGCCCTTCTT 
GCAGAATTAG AGGAGCAAAA AACTGTTAAA GCCAAACTCA GAGAGACGTT GACTTTCTTT 
GATGAGCTTG AAAACATCGG CAGGTATCAA GGAACTAGTA ACTTTAGGGA GAGTTTGGTG 
TCTCTGGTCC AGAACTGCAG AAAACTCCAG GATATTAGAG ATAATGTAGA AAAAGAGAGC 
AAAAGGCTGG AAACACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Current biology : CB23(16)1514-26(2013 Aug)
Shrestha RL,Draviam VM

The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation.
The Journal of cell biology173(1)9-17(2006 Apr)
Kline SL,Cheeseman IM,Hori T,Fukagawa T,Desai A

A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.
Genes & development18(18)2255-68(2004 Sep)
Cheeseman IM,Niessen S,Anderson S,Hyndman F,Yates JR 3rd,Oegema K,Desai A

Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway.
The Journal of cell biology160(1)25-39(2003 Jan)
Goshima G,Kiyomitsu T,Yoda K,Yanagida M

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Mis12 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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