ORF cDNA Clones and Custom Clones » Species Summary » Rattus norvegicus » Ptafr cDNA ORF clone

Ptafr cDNA ORF clone, Rattus norvegicus(Norway rat)

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Summary

Gene Symbol	Ptafr
Entrez Gene ID	58949
Full Name	platelet-activating factor receptor
General protein information	Preferred Names platelet-activating factor receptor Names platelet-activating factor receptor PAF-R PAFr
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	acts as a receptor to mediate the biological effects of platelet-activating factor [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_053321.2	NP_445773.1	platelet-activating factor receptor
XM_006239064.2	XP_006239126.1	platelet-activating factor receptor isoform X1
XM_006239063.3	XP_006239125.1	platelet-activating factor receptor isoform X1

Homologies

	PTAFR	XP_001112942.2
Canis lupus familiaris (dog)	PTAFR	XP_005617766.1
Rattus norvegicus (Norway rat)	Ptafr	NP_445773.1
Xenopus tropicalis (tropical clawed frog)	ptafr	NP_001008003.1
Homo sapiens (human)	PTAFR	NP_000943.1
Pan troglodytes (chimpanzee)	PTAFR	XP_001151906.1
Bos taurus (cattle)	PTAFR	NP_001035628.1
Mus musculus (house mouse)	Ptafr	NP_001074680.1
Danio rerio (zebrafish)	LOC100002627	XP_001342372.1
Gallus gallus (chicken)	PTAFR	XP_004947758.1

Bibliography

Related articles in PubMed

PAF enhances MMP-2 production in rat aortic VSMCs via a ?-arrestin2-dependent ERK signaling pathway.
Kim YH, Lee SJ, Seo KW, Bae JU, Park SY, Kim EK, Bae SS, Kim JH, Kim CD
Journal of lipid research54(10)2678-86(2013 Oct)

Up-regulation of platelet-activating factor synthases and its receptor in spinal cord contribute to development of neuropathic pain following peripheral nerve injury.
Okubo M, Yamanaka H, Kobayashi K, Kanda H, Dai Y, Noguchi K
Molecular pain88(2012 Feb)

Anti-ischaemic effects of bilobalide on neonatal rat cardiomyocytes and the involvement of the platelet-activating factor receptor.
Maerz S, Liu CH, Guo W, Zhu YZ
Bioscience reports31(5)439-47(2011 Oct)

Mechanism and dose-effect of Ginkgolide B on severe acute pancreatitis of rats.
Ji RL, Xia SH, Di Y, Xu W
World journal of gastroenterology17(17)2241-7(2011 May)

Platelet activating factor blocks interkinetic nuclear migration in retinal progenitors through an arrest of the cell cycle at the S/G2 transition.
Fragel-Madeira L, Meletti T, Mariante RM, Monteiro RQ, Einicker-Lamas M, Bernardo RR, Lopes AH, Linden R
PloS one6(1)e16058(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show the histological localization of PAF synthases and its receptor in the spinal cord following peripheral nerve injury.
Title: Up-regulation of platelet-activating factor synthases and its receptor in spinal cord contribute to development of neuropathic pain following peripheral nerve injury.

In hypoxic tissue, PAFR protein expression is up-regulated, but is reduced in the bilobalide treated tissue.
Title: Anti-ischaemic effects of bilobalide on neonatal rat cardiomyocytes and the involvement of the platelet-activating factor receptor.

PAF inhibition interkinetic nuclear migration (IKNM) in retinal proliferating progenitors depended on the PAF receptor, Erk and p38 pathways and Chk1
Title: Platelet activating factor blocks interkinetic nuclear migration in retinal progenitors through an arrest of the cell cycle at the S/G2 transition.

Cirrhosis sensitizes hepatic stellate cells to PAF by elevating its receptor level
Title: Hepatic stellate cells may be potential effectors of platelet activating factor induced portal hypertension.

Platelet activating factor receptor (PAF-R) plays an important role in occurrence and development of severe acute pancreatitis. BN52021 exerts biological effects through competitively inhibiting the binding of increased both PAF and PAF-R expression.
Title: Significance of platelet activating factor receptor expression in pancreatic tissues of rats with severe acute pancreatitis and effects of BN52021.

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The following Ptafr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptafr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09269	NM_053321.2 Latest version!	Rattus norvegicus platelet-activating factor receptor (Ptafr), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$223.30 $319.00
ORa09269	XM_006239064.2 Latest version!	Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$223.30 $319.00
ORa09269	XM_006239063.3 Latest version!	Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptafr ( NM_053321.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445773.1 Rattus norvegicus platelet-activating factor receptor (Ptafr), mRNA.

Price & Availability↑

CloneID	ORa09269
Clone ID Related Accession (Same CDS sequence)	NM_053321.2 , XM_006239064.2 , XM_006239063.3
Accession Version	NM_053321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Rattus norvegicus(Norway rat)
Product	platelet-activating factor receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072491.1. On Jun 13, 2004 this sequence version replaced NM_053321.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072491.1, U04740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445773.1
CDS	99..1124
Translation	MEQNGSFRVDSEFRYTLFPIVYSVIFVLGVVANGYVLWVFATLYPSKKLNEIKIFMVNLTVADLLFLMTLPLWIVYYSNEGDWIVHKFLCNLAGCLFFINTYCSVAFLGVITYNRYQAVAYPIKTAQATTRKRGITLSLVIWISIAATASYFLATDSTNVVPKKDGSGNITRCFEHYEPYSVPILVVHIFITSCFFLVFFLIFYCNMVIIHTLLTRPVRQQRKPEVKRRALWMVCTVLAVFVICFVPHHVVQLPWTLAELGYQTNFHQAINDAHQITLCLLSTNCVLDPVIYCFLTKKFRKHLSEKFYSMRSSRKCSRATSDTCTEVMMPANQTPVLPLKN

Target ORF information:

RefSeq Version	NM_053321.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus platelet-activating factor receptor (Ptafr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053321.2

ORF Insert Sequence:

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptafr ( XM_006239064.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239126.1 Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09269
Clone ID Related Accession (Same CDS sequence)	NM_053321.2 , XM_006239064.2 , XM_006239063.3
Accession Version	XM_006239064.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	platelet-activating factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006239064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239126.1
CDS	710..1735
Translation	MEQNGSFRVDSEFRYTLFPIVYSVIFVLGVVANGYVLWVFATLYPSKKLNEIKIFMVNLTVADLLFLMTLPLWIVYYSNEGDWIVHKFLCNLAGCLFFINTYCSVAFLGVITYNRYQAVAYPIKTAQATTRKRGITLSLVIWISIAATASYFLATDSTNVVPKKDGSGNITRCFEHYEPYSVPILVVHIFITSCFFLVFFLIFYCNMVIIHTLLTRPVRQQRKPEVKRRALWMVCTVLAVFVICFVPHHVVQLPWTLAELGYQTNFHQAINDAHQITLCLLSTNCVLDPVIYCFLTKKFRKHLSEKFYSMRSSRKCSRATSDTCTEVMMPANQTPVLPLKN

Target ORF information:

RefSeq Version	XM_006239064.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239064.2

ORF Insert Sequence:

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptafr ( XM_006239063.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239125.1 Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa09269
Clone ID Related Accession (Same CDS sequence)	NM_053321.2 , XM_006239064.2 , XM_006239063.3
Accession Version	XM_006239063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	platelet-activating factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239125.1
CDS	434..1459
Translation	MEQNGSFRVDSEFRYTLFPIVYSVIFVLGVVANGYVLWVFATLYPSKKLNEIKIFMVNLTVADLLFLMTLPLWIVYYSNEGDWIVHKFLCNLAGCLFFINTYCSVAFLGVITYNRYQAVAYPIKTAQATTRKRGITLSLVIWISIAATASYFLATDSTNVVPKKDGSGNITRCFEHYEPYSVPILVVHIFITSCFFLVFFLIFYCNMVIIHTLLTRPVRQQRKPEVKRRALWMVCTVLAVFVICFVPHHVVQLPWTLAELGYQTNFHQAINDAHQITLCLLSTNCVLDPVIYCFLTKKFRKHLSEKFYSMRSSRKCSRATSDTCTEVMMPANQTPVLPLKN

Target ORF information:

RefSeq Version	XM_006239063.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus platelet-activating factor receptor (Ptafr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239063.3

ORF Insert Sequence:

ATGGAGCAAA ATGGCTCCTT CCGTGTGGAT TCTGAGTTTC GATACACCCT CTTTCCGATC 
GTTTACAGCG TCATCTTTGT ACTGGGCGTG GTTGCCAATG GCTATGTGCT GTGGGTCTTT 
GCCACCTTGT ACCCGTCCAA GAAACTGAAT GAGATAAAGA TCTTCATGGT GAATCTCACC 
GTGGCGGACC TGCTCTTCTT GATGACCCTC CCGCTGTGGA TTGTCTATTA CTCCAACGAG 
GGCGACTGGA TTGTACACAA ATTCCTGTGC AACCTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT CCTTGGCGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACCGCCCA GGCCACCACC CGCAAGCGTG GCATCACTTT GTCCCTGGTC 
ATTTGGATAT CCATCGCGGC TACTGCATCC TACTTCCTGG CCACAGACTC TACCAACGTG 
GTGCCCAAAA AGGATGGCTC AGGTAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTCGT CCACATCTTC ATCACCTCCT GTTTCTTCCT CGTCTTCTTC 
CTCATCTTCT ACTGCAACAT GGTCATCATC CACACGCTGC TCACGCGGCC TGTGCGGCAG 
CAGCGCAAAC CGGAAGTGAA GCGCCGGGCG CTGTGGATGG TCTGCACTGT CTTGGCGGTA 
TTTGTCATCT GCTTTGTGCC CCATCACGTG GTCCAGCTAC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ACCAAATCAC CCTCTGCCTC 
CTGAGCACCA ACTGCGTCTT AGATCCTGTT ATCTACTGCT TTCTCACCAA GAAGTTCCGG 
AAGCACCTCA GCGAAAAGTT TTACAGCATG CGAAGTAGCC GGAAGTGCTC CCGAGCCACT 
AGCGACACGT GCACCGAGGT GATGATGCCA GCCAACCAGA CTCCTGTCCT GCCATTGAAA 
AATTAA