• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

Kcnt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Kcnt1
Entrez Gene ID 60444
Full Name potassium sodium-activated channel subfamily T member 1
Synonyms Slack,rSlo2
General protein information
Preferred Names

potassium sodium-activated channel subfamily T member 1

Names

potassium channel subfamily T member 1
potassium channel subunit (Slack)
potassium channel, sodium-activated subfamily T, member 1
potassium channel, subfamily T, member 1
sequence like a calcium-activated potassium channel subunit
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

3

3p13
Summary Na(+)-activated potassium channel; may be involved in regulating the firing properties of neurons [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006233697.3 XP_006233759.1 potassium channel subfamily T member 1 isoform X1
XM_006233698.3 XP_006233760.1 potassium channel subfamily T member 1 isoform X2
XM_006233705.3 XP_006233767.1 potassium channel subfamily T member 1 isoform X9
XM_006233706.3 XP_006233768.1 potassium channel subfamily T member 1 isoform X10
XM_008761624.2 XP_008759846.1 potassium channel subfamily T member 1 isoform X13
XM_006233699.3 XP_006233761.1 potassium channel subfamily T member 1 isoform X3
XM_006233702.3 XP_006233764.1 potassium channel subfamily T member 1 isoform X6
XM_017592015.1 XP_017447504.1 potassium channel subfamily T member 1 isoform X12
NM_021853.1 NP_068625.1 potassium channel subfamily T member 1
XM_006233700.3 XP_006233762.1 potassium channel subfamily T member 1 isoform X4
XM_006233704.3 XP_006233766.1 potassium channel subfamily T member 1 isoform X8
XM_006233701.3 XP_006233763.1 potassium channel subfamily T member 1 isoform X5
XM_006233703.3 XP_006233765.1 potassium channel subfamily T member 1 isoform X7
XM_006233707.3 XP_006233769.1 potassium channel subfamily T member 1 isoform X11

Pathways from BioSystems


Homologies


Homo sapiens (human) KCNT1 NP_065873.2
Caenorhabditis elegans (roundworm) slo-2 NP_001129924.1
Xenopus tropicalis (tropical clawed frog) kcnt2 NP_001163984.2
LOC721830 XP_001118025.2
Mus musculus (house mouse) Kcnt1 NP_780671.2
Pan troglodytes (chimpanzee) KCNT1 XP_003312453.2
Canis lupus familiaris (dog) KCNT1 XP_005625191.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007585 XP_001687885.2
Drosophila melanogaster (fruit fly) CG42732 NP_001097259.2
Bos taurus (cattle) KCNT1 XP_002691660.3
Rattus norvegicus (Norway rat) Kcnt1 NP_068625.1
Gallus gallus (chicken) KCNT1 NP_989893.1
Danio rerio (zebrafish) kcnt1 XP_001920632.3

Gene Ontology


Bibliography

Related articles in PubMed

The Phe932Ile mutation in KCNT1 channels associated with severe epilepsy, delayed myelination and leukoencephalopathy produces a loss-of-function channel phenotype.
Evely KM, Pryce KD, Bhattacharjee A
Neuroscience35165-70(2017 May)

Heteromeric Slick/Slack K+ channels show graded sensitivity to cell volume changes.
Tejada MA, Hashem N, Calloe K, Klaerke DA
PloS one12(2)e0169914(2017)

Slack sodium-activated potassium channel membrane expression requires p38 mitogen-activated protein kinase phosphorylation.
Gururaj S, Fleites J, Bhattacharjee A
Neuropharmacology103279-89(2016 Apr)

Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.
Tejada MA, Stople K, Hammami Bomholtz S, Meinild AK, Poulsen AN, Klaerke DA
PloS one9(10)e110833(2014)

cAMP-dependent kinase does not modulate the Slack sodium-activated potassium channel.
Nuwer MO, Picchione KE, Bhattacharjee A
Neuropharmacology57(3)219-26(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mRNA of aquaporin 1 was co-expressed in Xenopus oocytes with homomeric or heteromeric Slick and Slack alpha-subunits. Hypotonic or hypertonic buffers caused changes in current. The heteromeric channel had a characteristic graded sensitivity to small and fast changes in cell volume. This the cell volume sensitivity is dependent on the number of volume sensitive Slick alpha-subunits in the tetrameric channels.
Title: Heteromeric Slick/Slack K+ channels show graded sensitivity to cell volume changes.

Results show that p38 MAPK is an obligate regulator of Slack channel function via the trafficking of channels into the membrane. The present study identifies Slack KNa channels as p38 MAPK substrates.
Title: Slack sodium-activated potassium channel membrane expression requires p38 mitogen-activated protein kinase phosphorylation.

Slick channels, in contrast to the similar Slack channels, are the only high-conductance K+ channels strongly sensitive to small changes in cell volume.
Title: Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.

phosphorylation by protein kinase A does not acutely modulate the function and gating activation kinetics of Slack channels
Title: cAMP-dependent kinase does not modulate the Slack sodium-activated potassium channel.

Heteromer formation requires the N-terminal domain of Slack-B, one of the alternative splice variants of the Slack channel.
Title: The N-terminal domain of Slack determines the formation and trafficking of Slick/Slack heteromeric sodium-activated potassium channels.

The following Kcnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa05286 NM_021853.1
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $909.30
$1299.00
ORa60528 XM_017592015.1
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
ORa25514 XM_006233697.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25515 XM_006233698.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25516 XM_006233699.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25517 XM_006233700.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25518 XM_006233701.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25519 XM_006233702.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25520 XM_006233703.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25521 XM_006233704.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25522 XM_006233705.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25523 XM_006233706.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa25524 XM_006233707.3
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa43525 XM_008761624.2
Latest version!		 Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa05286
Clone ID Related Accession (Same CDS sequence) NM_021853.1
Accession Version NM_021853.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF089730.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642611, SAMN02642612 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAATAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGTGGGGGC GGCGGCAAGC TGACTCTGCC CACTGAGAAC 
GGCTCTGGCA GTCGGCGTCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAA TCAGAGGATG AGGTGACACC CTCAGACGAC 
GAGGGGCTCT CTGTGGTTGA GTATGTGAAG GGCTACCCTC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCTTGCCT GTAAAGGCCC CCTTCTGCTG CCTGCGACTG 
GATAAGGGCT GCAAACACAA CAGCTATGAG GATGCCAAGG CCTACGGGTT CAAGAACAAG 
TTGATCATCG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGTGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGC TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTGCTGCAG TGTGGCATCA TCTATGCTGA CAATCTGGTG 
GTGGTGGACA AGGAGAGTAC CATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATTGTCAACG TGCAGACCAT GTTCAGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACGCACCCTT CCAACATGCG GTTCATGCAG TTCCGGGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AATGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG GGTGTTTAGT ATCAGCATGT TGGATACGCT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TGCTGGGCCT TGACACGACG 
CCAGGCTCTG GCTACCTCTG TGCTATGAAG GTAACTGAGG ATGACCTGTG GATCCGTACT 
TATGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCTG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCCTCTGAG CCTCATGACC TCAGAGCCCA GTCTCAGATC 
TCGGTGAACA TGGAGGATTG CGAGGACACT CGGGAGGCCA AGGGGCCCTG GGGCACACGG 
GCTGCGTCTG GCGGTGGCAG CACCCATGGC CGTCACGGGG GCAGTGCTGA CCCGGTGGAG 
CACCCACTAC TGCGCCGCAA GAGCCTGCAG TGGGCCCGCA AGCTGAGCCG CAAGAGCAGC 
AAGCAGGCGG GGAAGGCACC CATGACCACA GACTGGATCA CCCAGCAGCG ACTCAGCCTG 
TACCGGCGCT CAGAGCGCCA GGAGCTCTCG GAGCTGGTCA AGAACCGAAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGACGTA GCAAATTTAA CAGCCAGTGA TGTGATGAAT 
CGGGTAAACC TGGGATATTT GCAAGATGAG ATGAATGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAGC CCAACGATAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCACGTGACC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAAC TGTCATCCTG TAATCCTGAG ACCCGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068625.1
CDS1..3714
Translation

Target ORF information:

RefSeq Version NM_021853.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_021853.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAATAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGTGGGGGC GGCGGCAAGC TGACTCTGCC CACTGAGAAC 
GGCTCTGGCA GTCGGCGTCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAA TCAGAGGATG AGGTGACACC CTCAGACGAC 
GAGGGGCTCT CTGTGGTTGA GTATGTGAAG GGCTACCCTC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCTTGCCT GTAAAGGCCC CCTTCTGCTG CCTGCGACTG 
GATAAGGGCT GCAAACACAA CAGCTATGAG GATGCCAAGG CCTACGGGTT CAAGAACAAG 
TTGATCATCG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGTGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGC TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTGCTGCAG TGTGGCATCA TCTATGCTGA CAATCTGGTG 
GTGGTGGACA AGGAGAGTAC CATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATTGTCAACG TGCAGACCAT GTTCAGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACGCACCCTT CCAACATGCG GTTCATGCAG TTCCGGGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AATGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG GGTGTTTAGT ATCAGCATGT TGGATACGCT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TGCTGGGCCT TGACACGACG 
CCAGGCTCTG GCTACCTCTG TGCTATGAAG GTAACTGAGG ATGACCTGTG GATCCGTACT 
TATGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCTG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCCTCTGAG CCTCATGACC TCAGAGCCCA GTCTCAGATC 
TCGGTGAACA TGGAGGATTG CGAGGACACT CGGGAGGCCA AGGGGCCCTG GGGCACACGG 
GCTGCGTCTG GCGGTGGCAG CACCCATGGC CGTCACGGGG GCAGTGCTGA CCCGGTGGAG 
CACCCACTAC TGCGCCGCAA GAGCCTGCAG TGGGCCCGCA AGCTGAGCCG CAAGAGCAGC 
AAGCAGGCGG GGAAGGCACC CATGACCACA GACTGGATCA CCCAGCAGCG ACTCAGCCTG 
TACCGGCGCT CAGAGCGCCA GGAGCTCTCG GAGCTGGTCA AGAACCGAAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGACGTA GCAAATTTAA CAGCCAGTGA TGTGATGAAT 
CGGGTAAACC TGGGATATTT GCAAGATGAG ATGAATGATC ATCACCAGAA CACCCTTTCC 
TATGTACTCA TCAACCCCCC GCCAGACACA AGACTGGAGC CCAACGATAT TGTGTACCTC 
ATCCGTTCCG ACCCCCTGGC CCACGTGACC AGCAGCTCCC AGAGTCGGAA AAGCAGCTGC 
AGCAACAAAC TGTCATCCTG TAATCCTGAG ACCCGGGATG AGACCCAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa60528
Clone ID Related Accession (Same CDS sequence) XM_017592015.1
Accession Version XM_017592015.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X12
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GATGACTGGG TGGGGCTGCG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017447504.1
CDS309..3812
Translation

Target ORF information:

RefSeq Version XM_017592015.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017592015.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GATGACTGGG TGGGGCTGCG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25514
Clone ID Related Accession (Same CDS sequence) XM_006233697.3
Accession Version XM_006233697.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAATGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAGCC CAACGATATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CACGTGACCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAACT GTCATCCTGT 
AATCCTGAGA CCCGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233759.1
CDS309..4061
Translation

Target ORF information:

RefSeq Version XM_006233697.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233697.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGAGA TGAATGATCA TCACCAGAAC ACCCTTTCCT ATGTACTCAT CAACCCCCCG 
CCAGACACAA GACTGGAGCC CAACGATATT GTGTACCTCA TCCGTTCCGA CCCCCTGGCC 
CACGTGACCA GCAGCTCCCA GAGTCGGAAA AGCAGCTGCA GCAACAAACT GTCATCCTGT 
AATCCTGAGA CCCGGGATGA GACCCAGCTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25515
Clone ID Related Accession (Same CDS sequence) XM_006233698.3
Accession Version XM_006233698.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGTGGGGGC GGCGGCAAGC TGACTCTGCC CACTGAGAAC 
GGCTCTGGCA GTCGGCGTCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAA TCAGAGGATG AGGTGACACC CTCAGACGAC 
GAGGGGCTCT CTGTGGTTGA GTATGTGAAG GGCTACCCTC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCTTGCCT GTAAAGGCCC CCTTCTGCTG CCTGCGACTG 
GATAAGGGCT GCAAACACAA CAGCTATGAG GATGCCAAGG CCTACGGGTT CAAGAACAAG 
TTGATCATCG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGTGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGC TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTGCTGCAG TGTGGCATCA TCTATGCTGA CAATCTGGTG 
GTGGTGGACA AGGAGAGTAC CATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATTGTCAACG TGCAGACCAT GTTCAGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACGCACCCTT CCAACATGCG GTTCATGCAG TTCCGGGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AATGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG GGTGTTTAGT ATCAGCATGT TGGATACGCT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TGCTGGGCCT TGACACGACG 
CCAGGCTCTG GCTACCTCTG TGCTATGAAG GTAACTGAGG ATGACCTGTG GATCCGTACT 
TATGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCTG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCCTCTGAG CCTCATGACC TCAGAGCCCA GGGGGGCCCA 
GCCATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA TTGCGAGGAC 
ACTCGGGAGG CCAAGGGGCC CTGGGGCACA CGGGCTGCGT CTGGCGGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCGGTG GAGCACCCAC TACTGCGCCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG CCGCAAGAGC AGCAAGCAGG CGGGGAAGGC ACCCATGACC 
ACAGACTGGA TCACCCAGCA GCGACTCAGC CTGTACCGGC GCTCAGAGCG CCAGGAGCTC 
TCGGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGGCTGC CCACCACTGG CTACGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAATG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AGCCCAACGA TATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCACGTG 
ACCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AACTGTCATC CTGTAATCCT 
GAGACCCGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233760.1
CDS309..4055
Translation

Target ORF information:

RefSeq Version XM_006233698.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233698.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GTGGCCATGG ACCTGCAGAA CACAGATTGC 
CGGCCCTCCC AGGGTGGCAG TGGTGGGGGC GGCGGCAAGC TGACTCTGCC CACTGAGAAC 
GGCTCTGGCA GTCGGCGTCC CAGCATCGCA CCCGTTCTGG AGTTGGCAGA CAGCTCAGCC 
CTGTTGCCCT GCGACCTGCT GAGTGACCAA TCAGAGGATG AGGTGACACC CTCAGACGAC 
GAGGGGCTCT CTGTGGTTGA GTATGTGAAG GGCTACCCTC CCAACTCACC CTACATTGGC 
AGCTCCCCGA CTTTATGCCA CCTCTTGCCT GTAAAGGCCC CCTTCTGCTG CCTGCGACTG 
GATAAGGGCT GCAAACACAA CAGCTATGAG GATGCCAAGG CCTACGGGTT CAAGAACAAG 
TTGATCATCG TCTCTGCTGA GACGGCAGGC AACGGGCTCT ACAACTTCAT CGTGCCTCTG 
CGTGCCTACT ACCGGTCCCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT TGACAACAAG 
CCTGACCACC ACTTCCTGGA GGCCATCTGC TGCTTCCCCA TGGTCTACTA CATGGAGGGA 
TCCGTGGACA ACCTGGACAG CTTGCTGCAG TGTGGCATCA TCTATGCTGA CAATCTGGTG 
GTGGTGGACA AGGAGAGTAC CATGAGCGCT GAAGAGGACT ACATGGCAGA TGCCAAGACC 
ATTGTCAACG TGCAGACCAT GTTCAGGCTT TTCCCCAGTC TCAGCATCAC CACGGAGCTC 
ACGCACCCTT CCAACATGCG GTTCATGCAG TTCCGGGCCA AGGACAGCTA CTCTCTGGCT 
CTTTCCAAAC TTGAAAAGCA AGAACGGGAG AATGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCATTTG CTGCTGGTCG GGTGTTTAGT ATCAGCATGT TGGATACGCT GCTCTACCAG 
TCCTTCGTGA AGGACTACAT GATCACCATC ACCAGGCTGC TGCTGGGCCT TGACACGACG 
CCAGGCTCTG GCTACCTCTG TGCTATGAAG GTAACTGAGG ATGACCTGTG GATCCGTACT 
TATGGCCGCC TCTTCCAGAA ACTCTGCTCC TCCAGCGCTG AGATCCCCAT CGGCATCTAC 
AGGACCGAGT GCCATGTCTT CTCCTCTGAG CCTCATGACC TCAGAGCCCA GGGGGGCCCA 
GCCATAGCCC TCCTGCCTGC CCAGTCTCAG ATCTCGGTGA ACATGGAGGA TTGCGAGGAC 
ACTCGGGAGG CCAAGGGGCC CTGGGGCACA CGGGCTGCGT CTGGCGGTGG CAGCACCCAT 
GGCCGTCACG GGGGCAGTGC TGACCCGGTG GAGCACCCAC TACTGCGCCG CAAGAGCCTG 
CAGTGGGCCC GCAAGCTGAG CCGCAAGAGC AGCAAGCAGG CGGGGAAGGC ACCCATGACC 
ACAGACTGGA TCACCCAGCA GCGACTCAGC CTGTACCGGC GCTCAGAGCG CCAGGAGCTC 
TCGGAGCTGG TCAAGAACCG AATGAAGCAC CTGGGGCTGC CCACCACTGG CTACGAGGAC 
GTAGCAAATT TAACAGCCAG TGATGTGATG AATCGGGTAA ACCTGGGATA TTTGCAAGAT 
GAGATGAATG ATCATCACCA GAACACCCTT TCCTATGTAC TCATCAACCC CCCGCCAGAC 
ACAAGACTGG AGCCCAACGA TATTGTGTAC CTCATCCGTT CCGACCCCCT GGCCCACGTG 
ACCAGCAGCT CCCAGAGTCG GAAAAGCAGC TGCAGCAACA AACTGTCATC CTGTAATCCT 
GAGACCCGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25516
Clone ID Related Accession (Same CDS sequence) XM_006233699.3
Accession Version XM_006233699.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ATGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAGCCCAA CGATATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAC GTGACCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAACTGTC ATCCTGTAAT CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233761.1
CDS309..4028
Translation

Target ORF information:

RefSeq Version XM_006233699.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233699.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ATGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAGCCCAA CGATATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAC GTGACCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAACTGTC ATCCTGTAAT CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25517
Clone ID Related Accession (Same CDS sequence) XM_006233700.3
Accession Version XM_006233700.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGGGGGG CCCAGCCATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGATTGCGA GGACACTCGG GAGGCCAAGG GGCCCTGGGG CACACGGGCT 
GCGTCTGGCG GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC GGTGGAGCAC 
CCACTACTGC GCCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGCCGCAA GAGCAGCAAG 
CAGGCGGGGA AGGCACCCAT GACCACAGAC TGGATCACCC AGCAGCGACT CAGCCTGTAC 
CGGCGCTCAG AGCGCCAGGA GCTCTCGGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AATGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAGCCCA ACGATATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA CGTGACCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAACTGT CATCCTGTAA TCCTGAGACC CGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233762.1
CDS309..4019
Translation

Target ORF information:

RefSeq Version XM_006233700.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233700.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGGGGGG CCCAGCCATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGATTGCGA GGACACTCGG GAGGCCAAGG GGCCCTGGGG CACACGGGCT 
GCGTCTGGCG GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC GGTGGAGCAC 
CCACTACTGC GCCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGCCGCAA GAGCAGCAAG 
CAGGCGGGGA AGGCACCCAT GACCACAGAC TGGATCACCC AGCAGCGACT CAGCCTGTAC 
CGGCGCTCAG AGCGCCAGGA GCTCTCGGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AATGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAGCCCA ACGATATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA CGTGACCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAACTGT CATCCTGTAA TCCTGAGACC CGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25518
Clone ID Related Accession (Same CDS sequence) XM_006233701.3
Accession Version XM_006233701.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GATGAGATGA ATGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAGCCCAA CGATATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAC 
GTGACCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAACTGTC ATCCTGTAAT 
CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233763.1
CDS309..3998
Translation

Target ORF information:

RefSeq Version XM_006233701.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233701.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GATGAGATGA ATGATCATCA CCAGAACACC CTTTCCTATG TACTCATCAA CCCCCCGCCA 
GACACAAGAC TGGAGCCCAA CGATATTGTG TACCTCATCC GTTCCGACCC CCTGGCCCAC 
GTGACCAGCA GCTCCCAGAG TCGGAAAAGC AGCTGCAGCA ACAAACTGTC ATCCTGTAAT 
CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25519
Clone ID Related Accession (Same CDS sequence) XM_006233702.3
Accession Version XM_006233702.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGTCTCA GATCTCGGTG AACATGGAGG ATTGCGAGGA CACTCGGGAG 
GCCAAGGGGC CCTGGGGCAC ACGGGCTGCG TCTGGCGGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCGGT GGAGCACCCA CTACTGCGCC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GCCGCAAGAG CAGCAAGCAG GCGGGGAAGG CACCCATGAC CACAGACTGG 
ATCACCCAGC AGCGACTCAG CCTGTACCGG CGCTCAGAGC GCCAGGAGCT CTCGGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAT 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAGCCCAACG ATATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCACGT GACCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAACTGTCAT CCTGTAATCC TGAGACCCGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233764.1
CDS309..3986
Translation

Target ORF information:

RefSeq Version XM_006233702.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233702.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGTCTCA GATCTCGGTG AACATGGAGG ATTGCGAGGA CACTCGGGAG 
GCCAAGGGGC CCTGGGGCAC ACGGGCTGCG TCTGGCGGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCGGT GGAGCACCCA CTACTGCGCC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GCCGCAAGAG CAGCAAGCAG GCGGGGAAGG CACCCATGAC CACAGACTGG 
ATCACCCAGC AGCGACTCAG CCTGTACCGG CGCTCAGAGC GCCAGGAGCT CTCGGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA CGTAGCAAAT 
TTAACAGCCA GTGATGTGAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA TGAGATGAAT 
GATCATCACC AGAACACCCT TTCCTATGTA CTCATCAACC CCCCGCCAGA CACAAGACTG 
GAGCCCAACG ATATTGTGTA CCTCATCCGT TCCGACCCCC TGGCCCACGT GACCAGCAGC 
TCCCAGAGTC GGAAAAGCAG CTGCAGCAAC AAACTGTCAT CCTGTAATCC TGAGACCCGG 
GATGAGACCC AGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25520
Clone ID Related Accession (Same CDS sequence) XM_006233703.3
Accession Version XM_006233703.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X7
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAT GAGATGAATG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AGCCCAACGA TATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCACGTG ACCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AACTGTCATC CTGTAATCCT GAGACCCGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233765.1
CDS309..3965
Translation

Target ORF information:

RefSeq Version XM_006233703.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233703.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAT GAGATGAATG ATCATCACCA GAACACCCTT 
TCCTATGTAC TCATCAACCC CCCGCCAGAC ACAAGACTGG AGCCCAACGA TATTGTGTAC 
CTCATCCGTT CCGACCCCCT GGCCCACGTG ACCAGCAGCT CCCAGAGTCG GAAAAGCAGC 
TGCAGCAACA AACTGTCATC CTGTAATCCT GAGACCCGGG ATGAGACCCA GCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25521
Clone ID Related Accession (Same CDS sequence) XM_006233704.3
Accession Version XM_006233704.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X8
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGGGGGG CCCAGCCATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGATTGCGA GGACACTCGG GAGGCCAAGG GGCCCTGGGG CACACGGGCT 
GCGTCTGGCG GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC GGTGGAGCAC 
CCACTACTGC GCCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGCCGCAA GAGCAGCAAG 
CAGGCGGGGA AGGCACCCAT GACCACAGAC TGGATCACCC AGCAGCGACT CAGCCTGTAC 
CGGCGCTCAG AGCGCCAGGA GCTCTCGGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AATGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAGCCCA ACGATATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA CGTGACCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAACTGT CATCCTGTAA TCCTGAGACC CGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233766.1
CDS471..4121
Translation

Target ORF information:

RefSeq Version XM_006233704.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233704.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGGGGGG CCCAGCCATA GCCCTCCTGC CTGCCCAGTC TCAGATCTCG 
GTGAACATGG AGGATTGCGA GGACACTCGG GAGGCCAAGG GGCCCTGGGG CACACGGGCT 
GCGTCTGGCG GTGGCAGCAC CCATGGCCGT CACGGGGGCA GTGCTGACCC GGTGGAGCAC 
CCACTACTGC GCCGCAAGAG CCTGCAGTGG GCCCGCAAGC TGAGCCGCAA GAGCAGCAAG 
CAGGCGGGGA AGGCACCCAT GACCACAGAC TGGATCACCC AGCAGCGACT CAGCCTGTAC 
CGGCGCTCAG AGCGCCAGGA GCTCTCGGAG CTGGTCAAGA ACCGAATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGACGTAGCA AATTTAACAG CCAGTGATGT GATGAATCGG 
GTAAACCTGG GATATTTGCA AGATGAGATG AATGATCATC ACCAGAACAC CCTTTCCTAT 
GTACTCATCA ACCCCCCGCC AGACACAAGA CTGGAGCCCA ACGATATTGT GTACCTCATC 
CGTTCCGACC CCCTGGCCCA CGTGACCAGC AGCTCCCAGA GTCGGAAAAG CAGCTGCAGC 
AACAAACTGT CATCCTGTAA TCCTGAGACC CGGGATGAGA CCCAGCTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25522
Clone ID Related Accession (Same CDS sequence) XM_006233705.3
Accession Version XM_006233705.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X9
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGT GGCCATGGAC 
CTGCAGAACA CAGATTGCCG GCCCTCCCAG GGTGGCAGTG GTGGGGGCGG CGGCAAGCTG 
ACTCTGCCCA CTGAGAACGG CTCTGGCAGT CGGCGTCCCA GCATCGCACC CGTTCTGGAG 
TTGGCAGACA GCTCAGCCCT GTTGCCCTGC GACCTGCTGA GTGACCAATC AGAGGATGAG 
GTGACACCCT CAGACGACGA GGGGCTCTCT GTGGTTGAGT ATGTGAAGGG CTACCCTCCC 
AACTCACCCT ACATTGGCAG CTCCCCGACT TTATGCCACC TCTTGCCTGT AAAGGCCCCC 
TTCTGCTGCC TGCGACTGGA TAAGGGCTGC AAACACAACA GCTATGAGGA TGCCAAGGCC 
TACGGGTTCA AGAACAAGTT GATCATCGTC TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC 
AACTTCATCG TGCCTCTGCG TGCCTACTAC CGGTCCCGCC GGGAGCTCAA CCCCATTGTG 
CTGCTGCTTG ACAACAAGCC TGACCACCAC TTCCTGGAGG CCATCTGCTG CTTCCCCATG 
GTCTACTACA TGGAGGGATC CGTGGACAAC CTGGACAGCT TGCTGCAGTG TGGCATCATC 
TATGCTGACA ATCTGGTGGT GGTGGACAAG GAGAGTACCA TGAGCGCTGA AGAGGACTAC 
ATGGCAGATG CCAAGACCAT TGTCAACGTG CAGACCATGT TCAGGCTTTT CCCCAGTCTC 
AGCATCACCA CGGAGCTCAC GCACCCTTCC AACATGCGGT TCATGCAGTT CCGGGCCAAG 
GACAGCTACT CTCTGGCTCT TTCCAAACTT GAAAAGCAAG AACGGGAGAA TGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCATTTGCT GCTGGTCGGG TGTTTAGTAT CAGCATGTTG 
GATACGCTGC TCTACCAGTC CTTCGTGAAG GACTACATGA TCACCATCAC CAGGCTGCTG 
CTGGGCCTTG ACACGACGCC AGGCTCTGGC TACCTCTGTG CTATGAAGGT AACTGAGGAT 
GACCTGTGGA TCCGTACTTA TGGCCGCCTC TTCCAGAAAC TCTGCTCCTC CAGCGCTGAG 
ATCCCCATCG GCATCTACAG GACCGAGTGC CATGTCTTCT CCTCTGAGCC TCATGACCTC 
AGAGCCCAGT CTCAGATCTC GGTGAACATG GAGGATTGCG AGGACACTCG GGAGGCCAAG 
GGGCCCTGGG GCACACGGGC TGCGTCTGGC GGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CGGTGGAGCA CCCACTACTG CGCCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGCCGCA AGAGCAGCAA GCAGGCGGGG AAGGCACCCA TGACCACAGA CTGGATCACC 
CAGCAGCGAC TCAGCCTGTA CCGGCGCTCA GAGCGCCAGG AGCTCTCGGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAATGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAGCCC 
AACGATATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ACGTGACCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAACTG TCATCCTGTA ATCCTGAGAC CCGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233767.1
CDS471..4082
Translation

Target ORF information:

RefSeq Version XM_006233705.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233705.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGT GGCCATGGAC 
CTGCAGAACA CAGATTGCCG GCCCTCCCAG GGTGGCAGTG GTGGGGGCGG CGGCAAGCTG 
ACTCTGCCCA CTGAGAACGG CTCTGGCAGT CGGCGTCCCA GCATCGCACC CGTTCTGGAG 
TTGGCAGACA GCTCAGCCCT GTTGCCCTGC GACCTGCTGA GTGACCAATC AGAGGATGAG 
GTGACACCCT CAGACGACGA GGGGCTCTCT GTGGTTGAGT ATGTGAAGGG CTACCCTCCC 
AACTCACCCT ACATTGGCAG CTCCCCGACT TTATGCCACC TCTTGCCTGT AAAGGCCCCC 
TTCTGCTGCC TGCGACTGGA TAAGGGCTGC AAACACAACA GCTATGAGGA TGCCAAGGCC 
TACGGGTTCA AGAACAAGTT GATCATCGTC TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC 
AACTTCATCG TGCCTCTGCG TGCCTACTAC CGGTCCCGCC GGGAGCTCAA CCCCATTGTG 
CTGCTGCTTG ACAACAAGCC TGACCACCAC TTCCTGGAGG CCATCTGCTG CTTCCCCATG 
GTCTACTACA TGGAGGGATC CGTGGACAAC CTGGACAGCT TGCTGCAGTG TGGCATCATC 
TATGCTGACA ATCTGGTGGT GGTGGACAAG GAGAGTACCA TGAGCGCTGA AGAGGACTAC 
ATGGCAGATG CCAAGACCAT TGTCAACGTG CAGACCATGT TCAGGCTTTT CCCCAGTCTC 
AGCATCACCA CGGAGCTCAC GCACCCTTCC AACATGCGGT TCATGCAGTT CCGGGCCAAG 
GACAGCTACT CTCTGGCTCT TTCCAAACTT GAAAAGCAAG AACGGGAGAA TGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCATTTGCT GCTGGTCGGG TGTTTAGTAT CAGCATGTTG 
GATACGCTGC TCTACCAGTC CTTCGTGAAG GACTACATGA TCACCATCAC CAGGCTGCTG 
CTGGGCCTTG ACACGACGCC AGGCTCTGGC TACCTCTGTG CTATGAAGGT AACTGAGGAT 
GACCTGTGGA TCCGTACTTA TGGCCGCCTC TTCCAGAAAC TCTGCTCCTC CAGCGCTGAG 
ATCCCCATCG GCATCTACAG GACCGAGTGC CATGTCTTCT CCTCTGAGCC TCATGACCTC 
AGAGCCCAGT CTCAGATCTC GGTGAACATG GAGGATTGCG AGGACACTCG GGAGGCCAAG 
GGGCCCTGGG GCACACGGGC TGCGTCTGGC GGTGGCAGCA CCCATGGCCG TCACGGGGGC 
AGTGCTGACC CGGTGGAGCA CCCACTACTG CGCCGCAAGA GCCTGCAGTG GGCCCGCAAG 
CTGAGCCGCA AGAGCAGCAA GCAGGCGGGG AAGGCACCCA TGACCACAGA CTGGATCACC 
CAGCAGCGAC TCAGCCTGTA CCGGCGCTCA GAGCGCCAGG AGCTCTCGGA GCTGGTCAAG 
AACCGAATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGACGTAGC AAATTTAACA 
GCCAGTGATG TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAATGATCAT 
CACCAGAACA CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAGCCC 
AACGATATTG TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ACGTGACCAG CAGCTCCCAG 
AGTCGGAAAA GCAGCTGCAG CAACAAACTG TCATCCTGTA ATCCTGAGAC CCGGGATGAG 
ACCCAGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25523
Clone ID Related Accession (Same CDS sequence) XM_006233706.3
Accession Version XM_006233706.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X10
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGACT GGGTGGGGCT GCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233768.1
CDS309..3875
Translation

Target ORF information:

RefSeq Version XM_006233706.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233706.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCGCGGG CCAAGCTGCC GCGCTCACCG TCCGAGGGCA AGGCGGGTCC GGGGGACACC 
CCAGCCGGCT CTGCAGCCCC TGAGGAGCCG CACGGACTCA GCCCGCTACT GCCCACCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGGGG 
GGCCCAGCCA TAGCCCTCCT GCCTGCCCAG TCTCAGATCT CGGTGAACAT GGAGGATTGC 
GAGGACACTC GGGAGGCCAA GGGGCCCTGG GGCACACGGG CTGCGTCTGG CGGTGGCAGC 
ACCCATGGCC GTCACGGGGG CAGTGCTGAC CCGGTGGAGC ACCCACTACT GCGCCGCAAG 
AGCCTGCAGT GGGCCCGCAA GCTGAGCCGC AAGAGCAGCA AGCAGGCGGG GAAGGCACCC 
ATGACCACAG ACTGGATCAC CCAGCAGCGA CTCAGCCTGT ACCGGCGCTC AGAGCGCCAG 
GAGCTCTCGG AGCTGGTCAA GAACCGAATG AAGCACCTGG GGCTGCCCAC CACTGGCTAC 
GAGGACGTAG CAAATTTAAC AGCCAGTGAT GTGATGAATC GGGTAAACCT GGGATATTTG 
CAAGATGACT GGGTGGGGCT GCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa25524
Clone ID Related Accession (Same CDS sequence) XM_006233707.3
Accession Version XM_006233707.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X11
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGTCTCA GATCTCGGTG AACATGGAGG ATTGCGAGGA CACTCGGGAG 
GCCAAGGGGC CCTGGGGCAC ACGGGCTGCG TCTGGCGGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCGGT GGAGCACCCA CTACTGCGCC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GCCGCAAGAG CAGCAAGCAG GCGGGGAAGG CACCCATGAC CACAGACTGG 
ATCACCCAGC AGCGACTCAG CCTGTACCGG CGCTCAGAGC GCCAGGAGCT CTCGGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGATGA GATGAATGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAG 
CCCAACGATA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCACGTGAC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAA CTGTCATCCT GTAATCCTGA GACCCGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006233769.1
CDS469..4023
Translation

Target ORF information:

RefSeq Version XM_006233707.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006233707.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGGTCC AGGTGGAATT CTATGTCAAT 
GAGAACACCT TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT 
GTCCTGCTCG ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC 
ACGTTCAATG GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA 
ATGGCTCTGT GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC 
TTGGAGATGA TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC 
CTGTTCATCC CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AATGACTTCC ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC 
CTGTTCTGCA CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG 
CGGGCAGGTG GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA 
ACCGTGGGCT TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC 
CTGATCTGTG TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG 
ATGGAGCGGC AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT 
GTGGTCCTGT GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC 
TATGCCCACC CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT 
GTCCAGGTGC GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG 
GGCTCTGCCC TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC 
TTTATCCTCA GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT 
CGAGCCTGGG CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG 
CCCGAAAACA AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG 
TACGCCATGC TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG 
GTGCACACAT CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC 
GGGCGCTGCT CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG 
GAGTATGAGG GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG 
TGCCTCATTG GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG 
CACATCCTGG CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA 
GCTTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGCGGG GCCTGGCAGG GCAGGCACTT 
TACGAAGGGC CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGG GACAGTGGCC 
ATGGACCTGC AGAACACAGA TTGCCGGCCC TCCCAGGGTG GCAGTGGTGG GGGCGGCGGC 
AAGCTGACTC TGCCCACTGA GAACGGCTCT GGCAGTCGGC GTCCCAGCAT CGCACCCGTT 
CTGGAGTTGG CAGACAGCTC AGCCCTGTTG CCCTGCGACC TGCTGAGTGA CCAATCAGAG 
GATGAGGTGA CACCCTCAGA CGACGAGGGG CTCTCTGTGG TTGAGTATGT GAAGGGCTAC 
CCTCCCAACT CACCCTACAT TGGCAGCTCC CCGACTTTAT GCCACCTCTT GCCTGTAAAG 
GCCCCCTTCT GCTGCCTGCG ACTGGATAAG GGCTGCAAAC ACAACAGCTA TGAGGATGCC 
AAGGCCTACG GGTTCAAGAA CAAGTTGATC ATCGTCTCTG CTGAGACGGC AGGCAACGGG 
CTCTACAACT TCATCGTGCC TCTGCGTGCC TACTACCGGT CCCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTTGACAA CAAGCCTGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTC 
CCCATGGTCT ACTACATGGA GGGATCCGTG GACAACCTGG ACAGCTTGCT GCAGTGTGGC 
ATCATCTATG CTGACAATCT GGTGGTGGTG GACAAGGAGA GTACCATGAG CGCTGAAGAG 
GACTACATGG CAGATGCCAA GACCATTGTC AACGTGCAGA CCATGTTCAG GCTTTTCCCC 
AGTCTCAGCA TCACCACGGA GCTCACGCAC CCTTCCAACA TGCGGTTCAT GCAGTTCCGG 
GCCAAGGACA GCTACTCTCT GGCTCTTTCC AAACTTGAAA AGCAAGAACG GGAGAATGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCA TTTGCTGCTG GTCGGGTGTT TAGTATCAGC 
ATGTTGGATA CGCTGCTCTA CCAGTCCTTC GTGAAGGACT ACATGATCAC CATCACCAGG 
CTGCTGCTGG GCCTTGACAC GACGCCAGGC TCTGGCTACC TCTGTGCTAT GAAGGTAACT 
GAGGATGACC TGTGGATCCG TACTTATGGC CGCCTCTTCC AGAAACTCTG CTCCTCCAGC 
GCTGAGATCC CCATCGGCAT CTACAGGACC GAGTGCCATG TCTTCTCCTC TGAGCCTCAT 
GACCTCAGAG CCCAGTCTCA GATCTCGGTG AACATGGAGG ATTGCGAGGA CACTCGGGAG 
GCCAAGGGGC CCTGGGGCAC ACGGGCTGCG TCTGGCGGTG GCAGCACCCA TGGCCGTCAC 
GGGGGCAGTG CTGACCCGGT GGAGCACCCA CTACTGCGCC GCAAGAGCCT GCAGTGGGCC 
CGCAAGCTGA GCCGCAAGAG CAGCAAGCAG GCGGGGAAGG CACCCATGAC CACAGACTGG 
ATCACCCAGC AGCGACTCAG CCTGTACCGG CGCTCAGAGC GCCAGGAGCT CTCGGAGCTG 
GTCAAGAACC GAATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGATGA GATGAATGAT 
CATCACCAGA ACACCCTTTC CTATGTACTC ATCAACCCCC CGCCAGACAC AAGACTGGAG 
CCCAACGATA TTGTGTACCT CATCCGTTCC GACCCCCTGG CCCACGTGAC CAGCAGCTCC 
CAGAGTCGGA AAAGCAGCTG CAGCAACAAA CTGTCATCCT GTAATCCTGA GACCCGGGAT 
GAGACCCAGC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa43525
Clone ID Related Accession (Same CDS sequence) XM_008761624.2
Accession Version XM_008761624.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product potassium channel subfamily T member 1 isoform X13
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ATGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAGCCCAA CGATATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAC GTGACCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAACTGTC ATCCTGTAAT CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008759846.1
CDS471..4130
Translation

Target ORF information:

RefSeq Version XM_008761624.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus potassium sodium-activated channel subfamily T member 1 (Kcnt1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008761624.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAACGACC TGGACACAGA GGTACTGCCC TTGCCGCCAC GGTATAGATT CCGTGACCTG 
CTTTTAGGAG ACCAGACATT CCCGAACGAC GACAGGCTTC ATGTGGAAGA TTTCAGCCTG 
GACTCTTCCC TTTCTCAGGT CCAGGTGGAA TTCTATGTCA ATGAGAACAC CTTCAAAGAA 
CGCCTCAAGC TGTTCTTCAT CAAAAACCAG AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTCA AGCTCCTCAC CTGCCTGCTG TACATTGTCC GTGTCCTGCT CGACAACCCA 
GACCAGGGCA TCGGATGCTG GGGCTGCACG AAGTATAACT ACACGTTCAA TGGTTCGTCC 
TCGGAGTTCC ACTGGGCTCC CATCCTGTGG GTGGAGAGGA AAATGGCTCT GTGGGTGATC 
CAGGTCATTG TGGCCACAAT AAGCTTCTTA GAGACCATGC TCCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATATTCCAT GTGTCTTTCG TCTTGGAGAT GATCAACACA 
CTGCCCTTCA TCATCACGGT CTTCTGGCCA CCTCTCCGGA ACCTGTTCAT CCCTGTGTTT 
CTCAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAATGACTT CCACCGTGCC 
ATCCTGCGCA CACAGTCAGC CATGTTCAAC CAGGTGCTCA TCCTGTTCTG CACCCTGCTG 
TGCCTGGTCT TCACAGGGAC CTGTGGGATT CAGCACTTAG AGCGGGCAGG TGGCAACTTG 
AACTTGCTGA CCTCCTTCTA CTTCTGCATT GTGACTTTCT CAACCGTGGG CTTCGGTGAT 
GTGACACCCA AGATCTGGCC ATCCCAGCTC CTGGTGGTCA TCCTGATCTG TGTCACCCTT 
GTGGTGCTCC CACTGCAGTT TGAAGAGCTT GTCTACCTCT GGATGGAGCG GCAGAAGTCA 
GGGGGCAACT ATAGCCGCCA CCGAGCACGG ACAGAGAAGC ATGTGGTCCT GTGTGTGAGC 
TCCCTCAAGA TCGATCTCCT CATGGATTTC CTGAATGAGT TCTATGCCCA CCCCCGTCTC 
CAGGACTACT ACGTGGTCAT CCTGTGTCCC TCGGAAATGG ATGTCCAGGT GCGCAGGGTC 
CTACAGATTC CCCTGTGGTC CCAGCGTGTC ATCTACCTCC AGGGCTCTGC CCTCAAGGAC 
CAGGACCTCA TGCGAGCTAA GATGGACAAC GGAGAGGCCT GCTTTATCCT CAGCAGCAGG 
AATGAGGTGG ACCGCACAGC TGCGGATCAC CAGACCATCC TTCGAGCCTG GGCTGTGAAA 
GACTTTGCCC CCAACTGCCC CCTCTATGTC CAGATTCTCA AGCCCGAAAA CAAGTTTCAC 
GTCAAATTTG CTGACCATGT GGTGTGCGAG GAAGAGTGCA AGTACGCCAT GCTGGCCCTG 
AACTGCATCT GCCCGGCCAC CTCCACCCTC ATCACCCTGC TGGTGCACAC ATCCCGTGGC 
CAGGAAGGAC AGGAGTCACC AGAGCAGTGG CAGCGCATGT ACGGGCGCTG CTCGGGCAAC 
GAGGTGTACC ACATTCGCAT GGGTGACAGT AAATTCTTCC GGGAGTATGA GGGCAAGAGC 
TTCACCTACG CAGCCTTCCA CGCGCACAAG AAGTATGGGG TGTGCCTCAT TGGGCTGAAA 
CGTGAAGAGA ACAAGAGTAT CCTGCTGAAC CCGGGGCCAC GGCACATCCT GGCCGCCTCT 
GACACCTGCT TCTATATCAA TATTACCAAG GAGGAGAACT CAGCTTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGCG GGGCCTGGCA GGGCAGGCAC TTTACGAAGG GCCCTCCCGG 
CTCCCAGTGC ACAGCATCAT CGCCTCTATG GGGACAGTGG CCATGGACCT GCAGAACACA 
GATTGCCGGC CCTCCCAGGG TGGCAGTGGT GGGGGCGGCG GCAAGCTGAC TCTGCCCACT 
GAGAACGGCT CTGGCAGTCG GCGTCCCAGC ATCGCACCCG TTCTGGAGTT GGCAGACAGC 
TCAGCCCTGT TGCCCTGCGA CCTGCTGAGT GACCAATCAG AGGATGAGGT GACACCCTCA 
GACGACGAGG GGCTCTCTGT GGTTGAGTAT GTGAAGGGCT ACCCTCCCAA CTCACCCTAC 
ATTGGCAGCT CCCCGACTTT ATGCCACCTC TTGCCTGTAA AGGCCCCCTT CTGCTGCCTG 
CGACTGGATA AGGGCTGCAA ACACAACAGC TATGAGGATG CCAAGGCCTA CGGGTTCAAG 
AACAAGTTGA TCATCGTCTC TGCTGAGACG GCAGGCAACG GGCTCTACAA CTTCATCGTG 
CCTCTGCGTG CCTACTACCG GTCCCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTTGAC 
AACAAGCCTG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TCCCCATGGT CTACTACATG 
GAGGGATCCG TGGACAACCT GGACAGCTTG CTGCAGTGTG GCATCATCTA TGCTGACAAT 
CTGGTGGTGG TGGACAAGGA GAGTACCATG AGCGCTGAAG AGGACTACAT GGCAGATGCC 
AAGACCATTG TCAACGTGCA GACCATGTTC AGGCTTTTCC CCAGTCTCAG CATCACCACG 
GAGCTCACGC ACCCTTCCAA CATGCGGTTC ATGCAGTTCC GGGCCAAGGA CAGCTACTCT 
CTGGCTCTTT CCAAACTTGA AAAGCAAGAA CGGGAGAATG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CATTTGCTGC TGGTCGGGTG TTTAGTATCA GCATGTTGGA TACGCTGCTC 
TACCAGTCCT TCGTGAAGGA CTACATGATC ACCATCACCA GGCTGCTGCT GGGCCTTGAC 
ACGACGCCAG GCTCTGGCTA CCTCTGTGCT ATGAAGGTAA CTGAGGATGA CCTGTGGATC 
CGTACTTATG GCCGCCTCTT CCAGAAACTC TGCTCCTCCA GCGCTGAGAT CCCCATCGGC 
ATCTACAGGA CCGAGTGCCA TGTCTTCTCC TCTGAGCCTC ATGACCTCAG AGCCCAGTCT 
CAGATCTCGG TGAACATGGA GGATTGCGAG GACACTCGGG AGGCCAAGGG GCCCTGGGGC 
ACACGGGCTG CGTCTGGCGG TGGCAGCACC CATGGCCGTC ACGGGGGCAG TGCTGACCCG 
GTGGAGCACC CACTACTGCG CCGCAAGAGC CTGCAGTGGG CCCGCAAGCT GAGCCGCAAG 
AGCAGCAAGC AGGCGGGGAA GGCACCCATG ACCACAGACT GGATCACCCA GCAGCGACTC 
AGCCTGTACC GGCGCTCAGA GCGCCAGGAG CTCTCGGAGC TGGTCAAGAA CCGAATGAAG 
CACCTGGGGC TGCCCACCAC TGGCTACGAG GACGTAGCAA ATTTAACAGC CAGTGATGTG 
ATGAATCGGG TAAACCTGGG ATATTTGCAA GATGAGATGA ATGATCATCA CCAGAACACC 
CTTTCCTATG TACTCATCAA CCCCCCGCCA GACACAAGAC TGGAGCCCAA CGATATTGTG 
TACCTCATCC GTTCCGACCC CCTGGCCCAC GTGACCAGCA GCTCCCAGAG TCGGAAAAGC 
AGCTGCAGCA ACAAACTGTC ATCCTGTAAT CCTGAGACCC GGGATGAGAC CCAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The Phe932Ile mutation in KCNT1 channels associated with severe epilepsy, delayed myelination and leukoencephalopathy produces a loss-of-function channel phenotype.
Neuroscience35165-70(2017 May)
Evely KM,Pryce KD,Bhattacharjee A


book

Heteromeric Slick/Slack K+ channels show graded sensitivity to cell volume changes.
PloS one12(2)e0169914(2017)
Tejada MA,Hashem N,Calloe K,Klaerke DA


book

Slack sodium-activated potassium channel membrane expression requires p38 mitogen-activated protein kinase phosphorylation.
Neuropharmacology103279-89(2016 Apr)
Gururaj S,Fleites J,Bhattacharjee A


book

Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.
PloS one9(10)e110833(2014)
Tejada MA,Stople K,Hammami Bomholtz S,Meinild AK,Poulsen AN,Klaerke DA


book

cAMP-dependent kinase does not modulate the Slack sodium-activated potassium channel.
Neuropharmacology57(3)219-26(2009 Sep)
Nuwer MO,Picchione KE,Bhattacharjee A