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Phka2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Phka2
Entrez Gene ID 678739
Full Name phosphorylase kinase regulatory subunit alpha 2
General protein information
Preferred Names

phosphorylase kinase regulatory subunit alpha 2

Names

phosphorylase b kinase regulatory subunit alpha, liver isoform
phosphorylase kinase, alpha 2
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

X

Xq14

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001190994.1 NP_001177923.1 phosphorylase b kinase regulatory subunit alpha, liver isoform
XM_008773195.2 XP_008771417.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_008773196.2 XP_008771418.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_008773197.2 XP_008771419.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
XM_017602153.1 XP_017457642.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
XM_017602154.1 XP_017457643.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
XM_008773194.2 XP_008771416.1 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1

Pathways from BioSystems


Homologies


PHKA2 XP_001084454.1
Rattus norvegicus (Norway rat) Phka2 NP_001177923.1
Danio rerio (zebrafish) LOC556199 XP_684044.4
Drosophila melanogaster (fruit fly) CG7766 NP_001138180.1
Pan troglodytes (chimpanzee) PHKA2 XP_003317431.1
Mus musculus (house mouse) Phka2 NP_001171350.1
Homo sapiens (human) PHKA2 NP_000283.1
Bos taurus (cattle) PHKA2 NP_001178474.1
Canis lupus familiaris (dog) PHKA2 XP_005641224.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000708 XP_559348.4
Caenorhabditis elegans (roundworm) C14B9.8 NP_498777.2
Gallus gallus (chicken) PHKA2 XP_004934744.1
Xenopus tropicalis (tropical clawed frog) phka2 XP_004911720.1

Gene Ontology


Bibliography

Related articles in PubMed

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Brushia RJ, Walsh DA
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)

Mutations in the phosphorylase kinase gene PHKA2 are responsible for X-linked liver glycogen storage disease.
Hendrickx J, Coucke P, Dams E, Lee P, Odi?vre M, Corbeel L, Fernandes JF, Willems PJ
Human molecular genetics4(1)77-83(1995 Jan)

Purification of rat liver phosphorylase kinase.
Chrisman TD, Jordan JE, Exton JH
The Journal of biological chemistry257(18)10798-804(1982 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phka2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phka2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa63098 XM_017602153.1
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
ORa63099 XM_017602154.1
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
ORa34922 NM_001190994.1
Latest version!		 Rattus norvegicus phosphorylase kinase regulatory subunit alpha 2 (Phka2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa49671 XM_008773194.2
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa49672 XM_008773195.2
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
ORa49673 XM_008773196.2
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
ORa49674 XM_008773197.2
Latest version!		 Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa63098
Clone ID Related Accession (Same CDS sequence) XM_017602153.1
Accession Version XM_017602153.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCGAGGTC TTCTCCAGTG TATGATGAGG CAGGTGGATA AAGTGGAGAA GTTCAAGCAT 
ACTCAGAGTA CCAAGGACAG TCTACATGCC AAGTACAACA CTGCCACATG CAGCACTGTG 
GTGGGTGATG ACCAGTGGGG CCACCTCCAG GTGGATGCCA CCTCCCTCTT CCTCCTGTTC 
CTGGCCCAGA TGACTGCCTC AGGTTTGCGT ATTATTTTTA CCCTCGACGA AGTGGCCTTC 
ATACAGAATC TTGTCTTCTA CATAGAGGCT GCATATAAAG TTGCTGATTA TGGAATGTGG 
GAACGTGGAG ATAAGACCAA TCAGGGCATT CCAGAATTGA ATGCAAGCTC AGTGGGAATG 
GCCAAGGCAG CACTCGAAGC AATTGATGAA CTAGATCTTT TTGGAGCCCA TGGAGGACGC 
AAATCGGTGA TCCATGTCCT GCCTGACGAA GTTGAGCACT GCCAGTCAAT TCTTTTCTCC 
ATGTTGCCGA GAGCATCAAC ATCTAAAGAA ATCGATGCTG GACTTCTTTC TATTATTTCT 
TTCCCGGCCT TTGCAGTAGA AGATGTGCAC CTTGTGAATG TGACCAAAAA TGAAATTATT 
TCCAAGCTTC AGGGGCGTTA CGGATGCTGT CGCTTCCTTC GGGATGGTTA TAAAACCCCA 
AGAGAGGACC CACACCGATT GCATTATGAC CCTGCTGAAC TTAAGCTTTT TGAAAACATT 
GAATGTGAAT GGCCTGTGTT CTGGACTTAT TTAATCATAG ATGGAGTCTT CAATGGTGAT 
GCTGTTCAGG TCCAGGAATA CCGAGAAGCC CTGGAAGGAA TATTAATCAG AGGCAAAGAT 
GGGATCCACT TGGTGCCAGA GCTCTATGCC ATCCCACCAG ACAAGGTGGA TGAAGAGTAT 
AAGAATCCAC ACACAGTAGA CCGAATTCCA CTGGGGAAGC TGCCCCATCT TTGGGGACAG 
TCCTTGTATA TCCTCAGCTC ACTGCTAGCC GAGGGTTTCC TTGCCACTGG TGAAATTGAT 
CCGTTAAATA GAAGATTTTC TACTTCAGTC AAACCTGATG TTGTAGTCCA AGTTGCTGTT 
TTGGCAGAAA ACAGTCACAT TAAGGGGCTC TTGAAGCAAC ATGGAATAAA TGTCCAGAGC 
ATTGCTGATG TGCATCCAAT TCGAGTCCAG CCAGGCCGGA TTCTTAGTCA CATATATGCC 
AAATTGGGAC AAAATAAGAA TATGAAATTG AGTGGTCGAC CATATCGGCA CATCGGGGTC 
CTTGGCACCT CTAAACTCTA TGTGATTAGG AATCACATCT TCACTTTTAC ACCCCAGTTC 
ACTGACCAGC ATCACTTCTA CCTGGCCCTG GACAACGAGA TGATTGTGGA GATGCTGAGG 
ATCGAACTGG CCTATCTGTG CACCTGCTGG CGGATGACTG GCCGCCCCAC TCTCACCTTC 
CCTGTCACAC ACACCATGCT CACAAATGAT GGCTCAGACA TTCATCCTGC AGTTCTTTCT 
ACAATTAGAA AACTGGAAGA TGGCTATTTT GGAGGTGCTA GAGTAAAACT AGGAATCCTG 
GCAGAGTTCC TTACTACCTC ATTCTACACA TACCTGACCT TCCTGGATCC AGACTGTGAT 
GAGAAGTTGT TTGGTGACAT CAGTGATAGG AGCTTTAGTC CTGACAGTGA GTCAGACCTG 
GGAGGATACC TTGAAGACAG CAGTCATCAA GAAAGCCAAG ATGAACTTGA CCAGTATATC 
AGTCACCTTC TTCAAAGCAC ATCCTTGAAG TGCTACCTGC CCCCTCTTTG TAAGAAGTCA 
GAAGACAGCC ATGTTTTCAG TGCTGTCCAC TCCACTCGGG ACATACTTTC TGTGATGGCC 
AAAGCAAAGG GTTTGGAAAC TCCATTTTTT CCCATGATTT TGCCAACTAG AGTTCTAAGT 
GGACACCGTA AGTCACTGAA TCTTGTTGAC TCCCCTCAGC CATTCCTAAA GAAGACTCCT 
GAATATGACT ACCAGTGGCC CAGAGATGAC CAGGATGAAG TGGACTGTGA GAAGCTAGTT 
GGGCAACTGA AAGACTGCTC AAACCTACAG GACCAAGCAG ACATTCTGTA CATTCTTTAT 
GTAATAAAGG GTCCCCGCTG GGATACCAAT TTGTTTGGAC AGCATGGAGT CACTGTTCAC 
AGTCTTCTCG GCGAGCTTTA TGGAAAAGCT GGCCTGAACC AAGAATGGAG TTTGATTCGC 
TACATTTCAG GCCTGCTCAG GAAGAAAGTG GAGGTCCTGG CTGAGGCCTG TGCAGATCTG 
CTGTCCCACC AGAAGCAGCT TACAGTGGGC CTGCCCCCTG AGCCCCGGGA GAAGACCATA 
TCTACGCCTC TTCCCCCAGA GGAGCTCACA GAACTCATAT ACGAAGCCAG TGGACAGGAC 
ATCAGCATTG CTGTCCTCAC ACAGGAGATC GTGGTTTACC TGGCCATGTA TGTCCGGGCC 
CAGCCTAGCC TCTTTGCAGA GATGCTCAGA CTCCGGATTG GATTGATCAT CCAGGTGATG 
GCCACAGAGC TGGCTCGGAG CCTGAACTGC TCAGGGGAAG AAGCTTCAGA GAGCTTGATG 
AACCTCAGCC CTTTCGACAT GAAGAGCCTT CTGCATCATA TACTGAGTGG GAAGGAGTTC 
GGCGTAGAGA GAAGTGTGCG CCCAATACAC TCCTCCATGT CCAGCCCTGC CATCTCCATC 
CATGAGGTGG GTCATACTGG AGTCACCAAA ACTGAGAGGA GTGGTATCAG CAGACTGAGG 
AGTGAGATGA AACAGATGAA TAGGCGAGCT AGTGCTGATG AGCAGAGGTC CAGCACACCG 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC ACCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CAGGAGAAGA TTGGATGGGG CTATCAACAG GGTCCCTGTG 
GGATTCTACC AGAGAGTGTG GAAGATCCTT CAGAAGTGCC ATGGTCTATC CATCGACGGT 
TATGTCCTCC CATCTTCAAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTCGCTGTC 
CATGTGGAAT CAGTGCTCAA CCGTGTGTCC CAGCCCGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACACT GCTCTCAGAT ACGGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTAGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ATATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCCA GTGGGGCTTA TGGCACAATG ACCTACCTAA CGAAAGCAGT AGCTTCTCAT 
TTGCAGGACC TGTTGCCCAG TTCAGGCTGC CAGATGCAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017457642.1
CDS1002..4403
Translation

Target ORF information:

RefSeq Version XM_017602153.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017602153.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCGAGGTC TTCTCCAGTG TATGATGAGG CAGGTGGATA AAGTGGAGAA GTTCAAGCAT 
ACTCAGAGTA CCAAGGACAG TCTACATGCC AAGTACAACA CTGCCACATG CAGCACTGTG 
GTGGGTGATG ACCAGTGGGG CCACCTCCAG GTGGATGCCA CCTCCCTCTT CCTCCTGTTC 
CTGGCCCAGA TGACTGCCTC AGGTTTGCGT ATTATTTTTA CCCTCGACGA AGTGGCCTTC 
ATACAGAATC TTGTCTTCTA CATAGAGGCT GCATATAAAG TTGCTGATTA TGGAATGTGG 
GAACGTGGAG ATAAGACCAA TCAGGGCATT CCAGAATTGA ATGCAAGCTC AGTGGGAATG 
GCCAAGGCAG CACTCGAAGC AATTGATGAA CTAGATCTTT TTGGAGCCCA TGGAGGACGC 
AAATCGGTGA TCCATGTCCT GCCTGACGAA GTTGAGCACT GCCAGTCAAT TCTTTTCTCC 
ATGTTGCCGA GAGCATCAAC ATCTAAAGAA ATCGATGCTG GACTTCTTTC TATTATTTCT 
TTCCCGGCCT TTGCAGTAGA AGATGTGCAC CTTGTGAATG TGACCAAAAA TGAAATTATT 
TCCAAGCTTC AGGGGCGTTA CGGATGCTGT CGCTTCCTTC GGGATGGTTA TAAAACCCCA 
AGAGAGGACC CACACCGATT GCATTATGAC CCTGCTGAAC TTAAGCTTTT TGAAAACATT 
GAATGTGAAT GGCCTGTGTT CTGGACTTAT TTAATCATAG ATGGAGTCTT CAATGGTGAT 
GCTGTTCAGG TCCAGGAATA CCGAGAAGCC CTGGAAGGAA TATTAATCAG AGGCAAAGAT 
GGGATCCACT TGGTGCCAGA GCTCTATGCC ATCCCACCAG ACAAGGTGGA TGAAGAGTAT 
AAGAATCCAC ACACAGTAGA CCGAATTCCA CTGGGGAAGC TGCCCCATCT TTGGGGACAG 
TCCTTGTATA TCCTCAGCTC ACTGCTAGCC GAGGGTTTCC TTGCCACTGG TGAAATTGAT 
CCGTTAAATA GAAGATTTTC TACTTCAGTC AAACCTGATG TTGTAGTCCA AGTTGCTGTT 
TTGGCAGAAA ACAGTCACAT TAAGGGGCTC TTGAAGCAAC ATGGAATAAA TGTCCAGAGC 
ATTGCTGATG TGCATCCAAT TCGAGTCCAG CCAGGCCGGA TTCTTAGTCA CATATATGCC 
AAATTGGGAC AAAATAAGAA TATGAAATTG AGTGGTCGAC CATATCGGCA CATCGGGGTC 
CTTGGCACCT CTAAACTCTA TGTGATTAGG AATCACATCT TCACTTTTAC ACCCCAGTTC 
ACTGACCAGC ATCACTTCTA CCTGGCCCTG GACAACGAGA TGATTGTGGA GATGCTGAGG 
ATCGAACTGG CCTATCTGTG CACCTGCTGG CGGATGACTG GCCGCCCCAC TCTCACCTTC 
CCTGTCACAC ACACCATGCT CACAAATGAT GGCTCAGACA TTCATCCTGC AGTTCTTTCT 
ACAATTAGAA AACTGGAAGA TGGCTATTTT GGAGGTGCTA GAGTAAAACT AGGAATCCTG 
GCAGAGTTCC TTACTACCTC ATTCTACACA TACCTGACCT TCCTGGATCC AGACTGTGAT 
GAGAAGTTGT TTGGTGACAT CAGTGATAGG AGCTTTAGTC CTGACAGTGA GTCAGACCTG 
GGAGGATACC TTGAAGACAG CAGTCATCAA GAAAGCCAAG ATGAACTTGA CCAGTATATC 
AGTCACCTTC TTCAAAGCAC ATCCTTGAAG TGCTACCTGC CCCCTCTTTG TAAGAAGTCA 
GAAGACAGCC ATGTTTTCAG TGCTGTCCAC TCCACTCGGG ACATACTTTC TGTGATGGCC 
AAAGCAAAGG GTTTGGAAAC TCCATTTTTT CCCATGATTT TGCCAACTAG AGTTCTAAGT 
GGACACCGTA AGTCACTGAA TCTTGTTGAC TCCCCTCAGC CATTCCTAAA GAAGACTCCT 
GAATATGACT ACCAGTGGCC CAGAGATGAC CAGGATGAAG TGGACTGTGA GAAGCTAGTT 
GGGCAACTGA AAGACTGCTC AAACCTACAG GACCAAGCAG ACATTCTGTA CATTCTTTAT 
GTAATAAAGG GTCCCCGCTG GGATACCAAT TTGTTTGGAC AGCATGGAGT CACTGTTCAC 
AGTCTTCTCG GCGAGCTTTA TGGAAAAGCT GGCCTGAACC AAGAATGGAG TTTGATTCGC 
TACATTTCAG GCCTGCTCAG GAAGAAAGTG GAGGTCCTGG CTGAGGCCTG TGCAGATCTG 
CTGTCCCACC AGAAGCAGCT TACAGTGGGC CTGCCCCCTG AGCCCCGGGA GAAGACCATA 
TCTACGCCTC TTCCCCCAGA GGAGCTCACA GAACTCATAT ACGAAGCCAG TGGACAGGAC 
ATCAGCATTG CTGTCCTCAC ACAGGAGATC GTGGTTTACC TGGCCATGTA TGTCCGGGCC 
CAGCCTAGCC TCTTTGCAGA GATGCTCAGA CTCCGGATTG GATTGATCAT CCAGGTGATG 
GCCACAGAGC TGGCTCGGAG CCTGAACTGC TCAGGGGAAG AAGCTTCAGA GAGCTTGATG 
AACCTCAGCC CTTTCGACAT GAAGAGCCTT CTGCATCATA TACTGAGTGG GAAGGAGTTC 
GGCGTAGAGA GAAGTGTGCG CCCAATACAC TCCTCCATGT CCAGCCCTGC CATCTCCATC 
CATGAGGTGG GTCATACTGG AGTCACCAAA ACTGAGAGGA GTGGTATCAG CAGACTGAGG 
AGTGAGATGA AACAGATGAA TAGGCGAGCT AGTGCTGATG AGCAGAGGTC CAGCACACCG 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC ACCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CAGGAGAAGA TTGGATGGGG CTATCAACAG GGTCCCTGTG 
GGATTCTACC AGAGAGTGTG GAAGATCCTT CAGAAGTGCC ATGGTCTATC CATCGACGGT 
TATGTCCTCC CATCTTCAAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTCGCTGTC 
CATGTGGAAT CAGTGCTCAA CCGTGTGTCC CAGCCCGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACACT GCTCTCAGAT ACGGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTAGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ATATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCCA GTGGGGCTTA TGGCACAATG ACCTACCTAA CGAAAGCAGT AGCTTCTCAT 
TTGCAGGACC TGTTGCCCAG TTCAGGCTGC CAGATGCAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa63099
Clone ID Related Accession (Same CDS sequence) XM_017602154.1
Accession Version XM_017602154.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCACTGG GCCAAGCTGT GCCCAAACGT TTACATTCCA TCAAGTCTGT GAGGTCCAGC 
ACACCGTCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACACCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCAGG AGAAGATTGG ATGGGGCTAT CAACAGGGTC 
CCTGTGGGAT TCTACCAGAG AGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTATCCATC 
GACGGTTATG TCCTCCCATC TTCAACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTC 
GCTGTCCATG TGGAATCAGT GCTCAACCGT GTGTCCCAGC CCGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACACTGCTC TCAGATACGG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTA GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGATAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCCAGTGG GGCTTATGGC ACAATGACCT ACCTAACGAA AGCAGTAGCT 
TCTCATTTGC AGGACCTGTT GCCCAGTTCA GGCTGCCAGA TGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017457643.1
CDS81..3788
Translation

Target ORF information:

RefSeq Version XM_017602154.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017602154.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCACTGG GCCAAGCTGT GCCCAAACGT TTACATTCCA TCAAGTCTGT GAGGTCCAGC 
ACACCGTCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACACCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCAGG AGAAGATTGG ATGGGGCTAT CAACAGGGTC 
CCTGTGGGAT TCTACCAGAG AGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTATCCATC 
GACGGTTATG TCCTCCCATC TTCAACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTC 
GCTGTCCATG TGGAATCAGT GCTCAACCGT GTGTCCCAGC CCGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACACTGCTC TCAGATACGG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTA GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGATAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCCAGTGG GGCTTATGGC ACAATGACCT ACCTAACGAA AGCAGTAGCT 
TCTCATTTGC AGGACCTGTT GCCCAGTTCA GGCTGCCAGA TGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa34922
Clone ID Related Accession (Same CDS sequence) NM_001190994.1
Accession Version NM_001190994.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-31
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform
Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07037997.1, AABR07037996.1, AABR07037995.1 and AABR07037943.1. On Jul 8, 2010 this sequence version replaced XM_001053483.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906350 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCACTGG GCCAAGCTGT GCCCAAACGT TTACATTCCA TCAAGTCTGC AAGGTCCAGC 
ACACCGTCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACACCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCAGG AGAAGATTGG ATGGGGCTAT CAACAGGGTC 
CCTGTGGGAT TCTACCAGAG AGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTATCCATC 
GACGGTTATG TCCTCCCATC TTCAACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTC 
GCTGTCCATG TGGAATCAGT GCTCAACCGT GTGTCCCAGC CCGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACACTGCTC TCAGATACGG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTA GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGATAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCCAGTGG GGCTTATGGC ACAATGACCT ACCTAACGAA AGCAGTAGCT 
TCTCATTTGC AGGACCTGTT GCCCAGTTCA GGCTGCCAGA TGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001177923.1
CDS1..3708
Translation

Target ORF information:

RefSeq Version NM_001190994.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase regulatory subunit alpha 2 (Phka2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001190994.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCACTGG GCCAAGCTGT GCCCAAACGT TTACATTCCA TCAAGTCTGC AAGGTCCAGC 
ACACCGTCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACACCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCAGG AGAAGATTGG ATGGGGCTAT CAACAGGGTC 
CCTGTGGGAT TCTACCAGAG AGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTATCCATC 
GACGGTTATG TCCTCCCATC TTCAACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTC 
GCTGTCCATG TGGAATCAGT GCTCAACCGT GTGTCCCAGC CCGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACACTGCTC TCAGATACGG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTA GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGATAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCCAGTGG GGCTTATGGC ACAATGACCT ACCTAACGAA AGCAGTAGCT 
TCTCATTTGC AGGACCTGTT GCCCAGTTCA GGCTGCCAGA TGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa49671
Clone ID Related Accession (Same CDS sequence) XM_008773194.2
Accession Version XM_008773194.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGAGG 
TCCAGCACAC CGTCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACACCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCAGGAGAA GATTGGATGG GGCTATCAAC 
AGGGTCCCTG TGGGATTCTA CCAGAGAGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTA 
TCCATCGACG GTTATGTCCT CCCATCTTCA ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTCGCTG TCCATGTGGA ATCAGTGCTC AACCGTGTGT CCCAGCCCGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACA CTGCTCTCAG ATACGGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGATATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC CAGTGGGGCT TATGGCACAA TGACCTACCT AACGAAAGCA 
GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008771416.1
CDS704..4357
Translation

Target ORF information:

RefSeq Version XM_008773194.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008773194.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGAGG 
TCCAGCACAC CGTCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACACCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCAGGAGAA GATTGGATGG GGCTATCAAC 
AGGGTCCCTG TGGGATTCTA CCAGAGAGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTA 
TCCATCGACG GTTATGTCCT CCCATCTTCA ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTCGCTG TCCATGTGGA ATCAGTGCTC AACCGTGTGT CCCAGCCCGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACA CTGCTCTCAG ATACGGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGATATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC CAGTGGGGCT TATGGCACAA TGACCTACCT AACGAAAGCA 
GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa49672
Clone ID Related Accession (Same CDS sequence) XM_008773195.2
Accession Version XM_008773195.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGAGG TCCAGCACAC CGTCCTCCCC GACAGGCACA 
TCATCTACAG ACTCTGGAGG ACACCACCTG GGCTGGGGAG AACAGCAGGG CCAGTGGCTG 
CGCAGGAGAA GATTGGATGG GGCTATCAAC AGGGTCCCTG TGGGATTCTA CCAGAGAGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTA TCCATCGACG GTTATGTCCT CCCATCTTCA 
ACAACCCAAG AGATGACCCC TTGTGAGATC AAGTTCGCTG TCCATGTGGA ATCAGTGCTC 
AACCGTGTGT CCCAGCCCGA GTATCGGCAG CTACTGGTAG AAGCCATCAT GGTACTGACA 
CTGCTCTCAG ATACGGAAAT GGACAGTATC GGGGGCATCA TCCATGTGGA CCAGATAGTG 
CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC CAGGTGTCAT TTGGAACCAC AGATATCCTG 
GAAAAAGACC AAGCCACAGG AATTTGCCAC CTCTTTTATG ACAGTGCTCC CAGTGGGGCT 
TATGGCACAA TGACCTACCT AACGAAAGCA GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC 
AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008771417.1
CDS704..4327
Translation

Target ORF information:

RefSeq Version XM_008773195.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008773195.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA TAAAGTGGAG 
AAGTTCAAGC ATACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGCAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TCCTGGCCCA GATGACTGCC TCAGGTTTGC GTATTATTTT TACCCTCGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAGG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGAGG TCCAGCACAC CGTCCTCCCC GACAGGCACA 
TCATCTACAG ACTCTGGAGG ACACCACCTG GGCTGGGGAG AACAGCAGGG CCAGTGGCTG 
CGCAGGAGAA GATTGGATGG GGCTATCAAC AGGGTCCCTG TGGGATTCTA CCAGAGAGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTA TCCATCGACG GTTATGTCCT CCCATCTTCA 
ACAACCCAAG AGATGACCCC TTGTGAGATC AAGTTCGCTG TCCATGTGGA ATCAGTGCTC 
AACCGTGTGT CCCAGCCCGA GTATCGGCAG CTACTGGTAG AAGCCATCAT GGTACTGACA 
CTGCTCTCAG ATACGGAAAT GGACAGTATC GGGGGCATCA TCCATGTGGA CCAGATAGTG 
CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC CAGGTGTCAT TTGGAACCAC AGATATCCTG 
GAAAAAGACC AAGCCACAGG AATTTGCCAC CTCTTTTATG ACAGTGCTCC CAGTGGGGCT 
TATGGCACAA TGACCTACCT AACGAAAGCA GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC 
AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa49673
Clone ID Related Accession (Same CDS sequence) XM_008773196.2
Accession Version XM_008773196.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCGTACC GTAAGAATGC TGACCGTGAT GAGGATAAAG CCAAGGCCTA TGAATTGGAA 
CAGAACGTAG TGAAGCTGAT GCGAGGTCTT CTCCAGTGTA TGATGAGGCA GGTGGATAAA 
GTGGAGAAGT TCAAGCATAC TCAGAGTACC AAGGACAGTC TACATGCCAA GTACAACACT 
GCCACATGCA GCACTGTGGT GGGTGATGAC CAGTGGGGCC ACCTCCAGGT GGATGCCACC 
TCCCTCTTCC TCCTGTTCCT GGCCCAGATG ACTGCCTCAG GTTTGCGTAT TATTTTTACC 
CTCGACGAAG TGGCCTTCAT ACAGAATCTT GTCTTCTACA TAGAGGCTGC ATATAAAGTT 
GCTGATTATG GAATGTGGGA ACGTGGAGAT AAGACCAATC AGGGCATTCC AGAATTGAAT 
GCAAGCTCAG TGGGAATGGC CAAGGCAGCA CTCGAAGCAA TTGATGAACT AGATCTTTTT 
GGAGCCCATG GAGGACGCAA ATCGGTGATC CATGTCCTGC CTGACGAAGT TGAGCACTGC 
CAGTCAATTC TTTTCTCCAT GTTGCCGAGA GCATCAACAT CTAAAGAAAT CGATGCTGGA 
CTTCTTTCTA TTATTTCTTT CCCGGCCTTT GCAGTAGAAG ATGTGCACCT TGTGAATGTG 
ACCAAAAATG AAATTATTTC CAAGCTTCAG GGGCGTTACG GATGCTGTCG CTTCCTTCGG 
GATGGTTATA AAACCCCAAG AGAGGACCCA CACCGATTGC ATTATGACCC TGCTGAACTT 
AAGCTTTTTG AAAACATTGA ATGTGAATGG CCTGTGTTCT GGACTTATTT AATCATAGAT 
GGAGTCTTCA ATGGTGATGC TGTTCAGGTC CAGGAATACC GAGAAGCCCT GGAAGGAATA 
TTAATCAGAG GCAAAGATGG GATCCACTTG GTGCCAGAGC TCTATGCCAT CCCACCAGAC 
AAGGTGGATG AAGAGTATAA GAATCCACAC ACAGTAGACC GAATTCCACT GGGGAAGCTG 
CCCCATCTTT GGGGACAGTC CTTGTATATC CTCAGCTCAC TGCTAGCCGA GGGTTTCCTT 
GCCACTGGTG AAATTGATCC GTTAAATAGA AGATTTTCTA CTTCAGTCAA ACCTGATGTT 
GTAGTCCAAG TTGCTGTTTT GGCAGAAAAC AGTCACATTA AGGGGCTCTT GAAGCAACAT 
GGAATAAATG TCCAGAGCAT TGCTGATGTG CATCCAATTC GAGTCCAGCC AGGCCGGATT 
CTTAGTCACA TATATGCCAA ATTGGGACAA AATAAGAATA TGAAATTGAG TGGTCGACCA 
TATCGGCACA TCGGGGTCCT TGGCACCTCT AAACTCTATG TGATTAGGAA TCACATCTTC 
ACTTTTACAC CCCAGTTCAC TGACCAGCAT CACTTCTACC TGGCCCTGGA CAACGAGATG 
ATTGTGGAGA TGCTGAGGAT CGAACTGGCC TATCTGTGCA CCTGCTGGCG GATGACTGGC 
CGCCCCACTC TCACCTTCCC TGTCACACAC ACCATGCTCA CAAATGATGG CTCAGACATT 
CATCCTGCAG TTCTTTCTAC AATTAGAAAA CTGGAAGATG GCTATTTTGG AGGTGCTAGA 
GTAAAACTAG GAATCCTGGC AGAGTTCCTT ACTACCTCAT TCTACACATA CCTGACCTTC 
CTGGATCCAG ACTGTGATGA GAAGTTGTTT GGTGACATCA GTGATAGGAG CTTTAGTCCT 
GACAGTGAGT CAGACCTGGG AGGATACCTT GAAGACAGCA GTCATCAAGA AAGCCAAGAT 
GAACTTGACC AGTATATCAG TCACCTTCTT CAAAGCACAT CCTTGAAGTG CTACCTGCCC 
CCTCTTTGTA AGAAGTCAGA AGACAGCCAT GTTTTCAGTG CTGTCCACTC CACTCGGGAC 
ATACTTTCTG TGATGGCCAA AGCAAAGGGT TTGGAAACTC CATTTTTTCC CATGATTTTG 
CCAACTAGAG TTCTAAGTGG ACACCGTAAG TCACTGAATC TTGTTGACTC CCCTCAGCCA 
TTCCTAAAGA AGACTCCTGA ATATGACTAC CAGTGGCCCA GAGATGACCA GGATGAAGTG 
GACTGTGAGA AGCTAGTTGG GCAACTGAAA GACTGCTCAA ACCTACAGGA CCAAGCAGAC 
ATTCTGTACA TTCTTTATGT AATAAAGGGT CCCCGCTGGG ATACCAATTT GTTTGGACAG 
CATGGAGTCA CTGTTCACAG TCTTCTCGGC GAGCTTTATG GAAAAGCTGG CCTGAACCAA 
GAATGGAGTT TGATTCGCTA CATTTCAGGC CTGCTCAGGA AGAAAGTGGA GGTCCTGGCT 
GAGGCCTGTG CAGATCTGCT GTCCCACCAG AAGCAGCTTA CAGTGGGCCT GCCCCCTGAG 
CCCCGGGAGA AGACCATATC TACGCCTCTT CCCCCAGAGG AGCTCACAGA ACTCATATAC 
GAAGCCAGTG GACAGGACAT CAGCATTGCT GTCCTCACAC AGGAGATCGT GGTTTACCTG 
GCCATGTATG TCCGGGCCCA GCCTAGCCTC TTTGCAGAGA TGCTCAGACT CCGGATTGGA 
TTGATCATCC AGGTGATGGC CACAGAGCTG GCTCGGAGCC TGAACTGCTC AGGGGAAGAA 
GCTTCAGAGA GCTTGATGAA CCTCAGCCCT TTCGACATGA AGAGCCTTCT GCATCATATA 
CTGAGTGGGA AGGAGTTCGG CGTAGAGAGA AGTGTGCGCC CAATACACTC CTCCATGTCC 
AGCCCTGCCA TCTCCATCCA TGAGGTGGGT CATACTGGAG TCACCAAAAC TGAGAGGAGT 
GGTATCAGCA GACTGAGGAG TGAGATGAAA CAGATGAATA GGCGAGCTAG TGCTGATGAG 
CAGAGGTCCA GCACACCGTC CTCCCCGACA GGCACATCAT CTACAGACTC TGGAGGACAC 
CACCTGGGCT GGGGAGAACA GCAGGGCCAG TGGCTGCGCA GGAGAAGATT GGATGGGGCT 
ATCAACAGGG TCCCTGTGGG ATTCTACCAG AGAGTGTGGA AGATCCTTCA GAAGTGCCAT 
GGTCTATCCA TCGACGGTTA TGTCCTCCCA TCTTCAACAA CCCAAGAGAT GACCCCTTGT 
GAGATCAAGT TCGCTGTCCA TGTGGAATCA GTGCTCAACC GTGTGTCCCA GCCCGAGTAT 
CGGCAGCTAC TGGTAGAAGC CATCATGGTA CTGACACTGC TCTCAGATAC GGAAATGGAC 
AGTATCGGGG GCATCATCCA TGTGGACCAG ATAGTGCAGC TAGCCAATCA GCTGTTCCTG 
CAGGACCAGG TGTCATTTGG AACCACAGAT ATCCTGGAAA AAGACCAAGC CACAGGAATT 
TGCCACCTCT TTTATGACAG TGCTCCCAGT GGGGCTTATG GCACAATGAC CTACCTAACG 
AAAGCAGTAG CTTCTCATTT GCAGGACCTG TTGCCCAGTT CAGGCTGCCA GATGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008771418.1
CDS932..4411
Translation

Target ORF information:

RefSeq Version XM_008773196.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008773196.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCGTACC GTAAGAATGC TGACCGTGAT GAGGATAAAG CCAAGGCCTA TGAATTGGAA 
CAGAACGTAG TGAAGCTGAT GCGAGGTCTT CTCCAGTGTA TGATGAGGCA GGTGGATAAA 
GTGGAGAAGT TCAAGCATAC TCAGAGTACC AAGGACAGTC TACATGCCAA GTACAACACT 
GCCACATGCA GCACTGTGGT GGGTGATGAC CAGTGGGGCC ACCTCCAGGT GGATGCCACC 
TCCCTCTTCC TCCTGTTCCT GGCCCAGATG ACTGCCTCAG GTTTGCGTAT TATTTTTACC 
CTCGACGAAG TGGCCTTCAT ACAGAATCTT GTCTTCTACA TAGAGGCTGC ATATAAAGTT 
GCTGATTATG GAATGTGGGA ACGTGGAGAT AAGACCAATC AGGGCATTCC AGAATTGAAT 
GCAAGCTCAG TGGGAATGGC CAAGGCAGCA CTCGAAGCAA TTGATGAACT AGATCTTTTT 
GGAGCCCATG GAGGACGCAA ATCGGTGATC CATGTCCTGC CTGACGAAGT TGAGCACTGC 
CAGTCAATTC TTTTCTCCAT GTTGCCGAGA GCATCAACAT CTAAAGAAAT CGATGCTGGA 
CTTCTTTCTA TTATTTCTTT CCCGGCCTTT GCAGTAGAAG ATGTGCACCT TGTGAATGTG 
ACCAAAAATG AAATTATTTC CAAGCTTCAG GGGCGTTACG GATGCTGTCG CTTCCTTCGG 
GATGGTTATA AAACCCCAAG AGAGGACCCA CACCGATTGC ATTATGACCC TGCTGAACTT 
AAGCTTTTTG AAAACATTGA ATGTGAATGG CCTGTGTTCT GGACTTATTT AATCATAGAT 
GGAGTCTTCA ATGGTGATGC TGTTCAGGTC CAGGAATACC GAGAAGCCCT GGAAGGAATA 
TTAATCAGAG GCAAAGATGG GATCCACTTG GTGCCAGAGC TCTATGCCAT CCCACCAGAC 
AAGGTGGATG AAGAGTATAA GAATCCACAC ACAGTAGACC GAATTCCACT GGGGAAGCTG 
CCCCATCTTT GGGGACAGTC CTTGTATATC CTCAGCTCAC TGCTAGCCGA GGGTTTCCTT 
GCCACTGGTG AAATTGATCC GTTAAATAGA AGATTTTCTA CTTCAGTCAA ACCTGATGTT 
GTAGTCCAAG TTGCTGTTTT GGCAGAAAAC AGTCACATTA AGGGGCTCTT GAAGCAACAT 
GGAATAAATG TCCAGAGCAT TGCTGATGTG CATCCAATTC GAGTCCAGCC AGGCCGGATT 
CTTAGTCACA TATATGCCAA ATTGGGACAA AATAAGAATA TGAAATTGAG TGGTCGACCA 
TATCGGCACA TCGGGGTCCT TGGCACCTCT AAACTCTATG TGATTAGGAA TCACATCTTC 
ACTTTTACAC CCCAGTTCAC TGACCAGCAT CACTTCTACC TGGCCCTGGA CAACGAGATG 
ATTGTGGAGA TGCTGAGGAT CGAACTGGCC TATCTGTGCA CCTGCTGGCG GATGACTGGC 
CGCCCCACTC TCACCTTCCC TGTCACACAC ACCATGCTCA CAAATGATGG CTCAGACATT 
CATCCTGCAG TTCTTTCTAC AATTAGAAAA CTGGAAGATG GCTATTTTGG AGGTGCTAGA 
GTAAAACTAG GAATCCTGGC AGAGTTCCTT ACTACCTCAT TCTACACATA CCTGACCTTC 
CTGGATCCAG ACTGTGATGA GAAGTTGTTT GGTGACATCA GTGATAGGAG CTTTAGTCCT 
GACAGTGAGT CAGACCTGGG AGGATACCTT GAAGACAGCA GTCATCAAGA AAGCCAAGAT 
GAACTTGACC AGTATATCAG TCACCTTCTT CAAAGCACAT CCTTGAAGTG CTACCTGCCC 
CCTCTTTGTA AGAAGTCAGA AGACAGCCAT GTTTTCAGTG CTGTCCACTC CACTCGGGAC 
ATACTTTCTG TGATGGCCAA AGCAAAGGGT TTGGAAACTC CATTTTTTCC CATGATTTTG 
CCAACTAGAG TTCTAAGTGG ACACCGTAAG TCACTGAATC TTGTTGACTC CCCTCAGCCA 
TTCCTAAAGA AGACTCCTGA ATATGACTAC CAGTGGCCCA GAGATGACCA GGATGAAGTG 
GACTGTGAGA AGCTAGTTGG GCAACTGAAA GACTGCTCAA ACCTACAGGA CCAAGCAGAC 
ATTCTGTACA TTCTTTATGT AATAAAGGGT CCCCGCTGGG ATACCAATTT GTTTGGACAG 
CATGGAGTCA CTGTTCACAG TCTTCTCGGC GAGCTTTATG GAAAAGCTGG CCTGAACCAA 
GAATGGAGTT TGATTCGCTA CATTTCAGGC CTGCTCAGGA AGAAAGTGGA GGTCCTGGCT 
GAGGCCTGTG CAGATCTGCT GTCCCACCAG AAGCAGCTTA CAGTGGGCCT GCCCCCTGAG 
CCCCGGGAGA AGACCATATC TACGCCTCTT CCCCCAGAGG AGCTCACAGA ACTCATATAC 
GAAGCCAGTG GACAGGACAT CAGCATTGCT GTCCTCACAC AGGAGATCGT GGTTTACCTG 
GCCATGTATG TCCGGGCCCA GCCTAGCCTC TTTGCAGAGA TGCTCAGACT CCGGATTGGA 
TTGATCATCC AGGTGATGGC CACAGAGCTG GCTCGGAGCC TGAACTGCTC AGGGGAAGAA 
GCTTCAGAGA GCTTGATGAA CCTCAGCCCT TTCGACATGA AGAGCCTTCT GCATCATATA 
CTGAGTGGGA AGGAGTTCGG CGTAGAGAGA AGTGTGCGCC CAATACACTC CTCCATGTCC 
AGCCCTGCCA TCTCCATCCA TGAGGTGGGT CATACTGGAG TCACCAAAAC TGAGAGGAGT 
GGTATCAGCA GACTGAGGAG TGAGATGAAA CAGATGAATA GGCGAGCTAG TGCTGATGAG 
CAGAGGTCCA GCACACCGTC CTCCCCGACA GGCACATCAT CTACAGACTC TGGAGGACAC 
CACCTGGGCT GGGGAGAACA GCAGGGCCAG TGGCTGCGCA GGAGAAGATT GGATGGGGCT 
ATCAACAGGG TCCCTGTGGG ATTCTACCAG AGAGTGTGGA AGATCCTTCA GAAGTGCCAT 
GGTCTATCCA TCGACGGTTA TGTCCTCCCA TCTTCAACAA CCCAAGAGAT GACCCCTTGT 
GAGATCAAGT TCGCTGTCCA TGTGGAATCA GTGCTCAACC GTGTGTCCCA GCCCGAGTAT 
CGGCAGCTAC TGGTAGAAGC CATCATGGTA CTGACACTGC TCTCAGATAC GGAAATGGAC 
AGTATCGGGG GCATCATCCA TGTGGACCAG ATAGTGCAGC TAGCCAATCA GCTGTTCCTG 
CAGGACCAGG TGTCATTTGG AACCACAGAT ATCCTGGAAA AAGACCAAGC CACAGGAATT 
TGCCACCTCT TTTATGACAG TGCTCCCAGT GGGGCTTATG GCACAATGAC CTACCTAACG 
AAAGCAGTAG CTTCTCATTT GCAGGACCTG TTGCCCAGTT CAGGCTGCCA GATGCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa49674
Clone ID Related Accession (Same CDS sequence) XM_008773197.2
Accession Version XM_008773197.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGAGG 
TCCAGCACAC CGTCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACACCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCAGGAGAA GATTGGATGG GGCTATCAAC 
AGGGTCCCTG TGGGATTCTA CCAGAGAGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTA 
TCCATCGACG GTTATGTCCT CCCATCTTCA ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTCGCTG TCCATGTGGA ATCAGTGCTC AACCGTGTGT CCCAGCCCGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACA CTGCTCTCAG ATACGGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGATATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC CAGTGGGGCT TATGGCACAA TGACCTACCT AACGAAAGCA 
GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_008771419.1
CDS705..4058
Translation

Target ORF information:

RefSeq Version XM_008773197.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphorylase kinase, alpha 2 (Phka2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_008773197.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCTAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA CATCTATAGC ATACTGGCTG TTTGGGGCCT GGGCATGGCG 
TACCGTAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAATT GGAACAGGAT 
TATGGAATGT GGGAACGTGG AGATAAGACC AATCAGGGCA TTCCAGAATT GAATGCAAGC 
TCAGTGGGAA TGGCCAAGGC AGCACTCGAA GCAATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCATGTC CTGCCTGACG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC GAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGC ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGT TACGGATGCT GTCGCTTCCT TCGGGATGGT 
TATAAAACCC CAAGAGAGGA CCCACACCGA TTGCATTATG ACCCTGCTGA ACTTAAGCTT 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAGTC 
TTCAATGGTG ATGCTGTTCA GGTCCAGGAA TACCGAGAAG CCCTGGAAGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAGCTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAATTC CACTGGGGAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA TATCCTCAGC TCACTGCTAG CCGAGGGTTT CCTTGCCACT 
GGTGAAATTG ATCCGTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTC 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TCTTGAAGCA ACATGGAATA 
AATGTCCAGA GCATTGCTGA TGTGCATCCA ATTCGAGTCC AGCCAGGCCG GATTCTTAGT 
CACATATATG CCAAATTGGG ACAAAATAAG AATATGAAAT TGAGTGGTCG ACCATATCGG 
CACATCGGGG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAACGA GATGATTGTG 
GAGATGCTGA GGATCGAACT GGCCTATCTG TGCACCTGCT GGCGGATGAC TGGCCGCCCC 
ACTCTCACCT TCCCTGTCAC ACACACCATG CTCACAAATG ATGGCTCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTGGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAATCC TGGCAGAGTT CCTTACTACC TCATTCTACA CATACCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGGTGAC ATCAGTGATA GGAGCTTTAG TCCTGACAGT 
GAGTCAGACC TGGGAGGATA CCTTGAAGAC AGCAGTCATC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGTCACCT TCTTCAAAGC ACATCCTTGA AGTGCTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATGTTTTC AGTGCTGTCC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCAAA GGGTTTGGAA ACTCCATTTT TTCCCATGAT TTTGCCAACT 
AGAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCATTCCTA 
AAGAAGACTC CTGAATATGA CTACCAGTGG CCCAGAGATG ACCAGGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATAAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG ACAGCATGGA 
GTCACTGTTC ACAGTCTTCT CGGCGAGCTT TATGGAAAAG CTGGCCTGAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTGG GCCTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTTCCCCCA GAGGAGCTCA CAGAACTCAT ATACGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGGGA AGAAGCTTCA 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATACTGAGT 
GGGAAGGAGT TCGGCGTAGA GAGAAGTGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGTCACCA AAACTGAGAG GAGTGGTATC 
AGCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGAGG 
TCCAGCACAC CGTCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACACCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCAGGAGAA GATTGGATGG GGCTATCAAC 
AGGGTCCCTG TGGGATTCTA CCAGAGAGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTA 
TCCATCGACG GTTATGTCCT CCCATCTTCA ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTCGCTG TCCATGTGGA ATCAGTGCTC AACCGTGTGT CCCAGCCCGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACA CTGCTCTCAG ATACGGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTAGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGATATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC CAGTGGGGCT TATGGCACAA TGACCTACCT AACGAAAGCA 
GTAGCTTCTC ATTTGCAGGA CCTGTTGCCC AGTTCAGGCT GCCAGATGCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)
Brushia RJ,Walsh DA


book

Mutations in the phosphorylase kinase gene PHKA2 are responsible for X-linked liver glycogen storage disease.
Human molecular genetics4(1)77-83(1995 Jan)
Hendrickx J,Coucke P,Dams E,Lee P,Odi?vre M,Corbeel L,Fernandes JF,Willems PJ


book

Purification of rat liver phosphorylase kinase.
The Journal of biological chemistry257(18)10798-804(1982 Sep)
Chrisman TD,Jordan JE,Exton JH