Pcgf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Pcgf5
Entrez Gene ID	681178
Full Name	polycomb group ring finger 5
General protein information	Preferred Names polycomb group ring finger 5 Names polycomb group RING finger protein 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231311.3	XP_006231373.1	polycomb group RING finger protein 5 isoform X2
XM_017589707.1	XP_017445196.1	polycomb group RING finger protein 5 isoform X1
NM_001129882.1	NP_001123354.1	polycomb group RING finger protein 5
XM_017589708.1	XP_017445197.1	polycomb group RING finger protein 5 isoform X1
XM_017589709.1	XP_017445198.1	polycomb group RING finger protein 5 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pcgf5b	NP_001038700.1
	LOC697020	XP_002805794.1
Bos taurus (cattle)	PCGF5	NP_001071448.1
Danio rerio (zebrafish)	pcgf5a	NP_001122184.1
Canis lupus familiaris (dog)	PCGF5	XP_005637589.1
Mus musculus (house mouse)	Pcgf5	NP_083784.1
Gallus gallus (chicken)	PCGF5	NP_001264290.1
Homo sapiens (human)	PCGF5	NP_001243478.1
Pan troglodytes (chimpanzee)	PCGF5	XP_003312724.1
Rattus norvegicus (Norway rat)	Pcgf5	NP_001123354.1
Xenopus tropicalis (tropical clawed frog)	pcgf5	XP_004915945.1

Gene Ontology
Bibliography

Related articles in PubMed

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Almeida M, Pintacuda G, Masui O, Koseki Y, Gdula M, Cerase A, Brown D, Mould A, Innocent C, Nakayama M, Schermelleh L, Nesterova TB, Koseki H, Brockdorff N
Science (New York, N.Y.)356(6342)1081-1084(2017 06)

An AUTS2-Polycomb complex activates gene expression in the CNS.
Gao Z, Lee P, Stafford JM, von Schimmelmann M, Schaefer A, Reinberg D
Nature516(7531)349-54(2014 Dec)

PRC1 complex diversity: where is it taking us?
Gil J, O'Loghlen A
Trends in cell biology24(11)632-41(2014 Nov)

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon JA, Kingston RE
Molecular cell49(5)808-24(2013 Mar)

Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.
Vandamme J, V?lkel P, Rosnoblet C, Le Faou P, Angrand PO
Molecular & cellular proteomics : MCP10(4)M110.002642(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcgf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcgf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02232	NM_001129882.1 Latest version!	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa02232	XM_006231311.3 Latest version!	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa24048	XM_017589708.1 Latest version!	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa24048	XM_017589709.1 Latest version!	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa24048	XM_017589707.1 Latest version!	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcgf5 ( NM_001129882.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001123354.1 Rattus norvegicus polycomb group ring finger 5 (Pcgf5), mRNA.

Price & Availability↑

CloneID	ORa02232
Clone ID Related Accession (Same CDS sequence)	XM_006231311.3 , NM_001129882.1
Accession Version	NM_001129882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167067.1. On or before Jul 10, 2008 this sequence version replaced XM_001080537.1, XM_001080543.1, XM_001080549.1. ##Evidence-Data-START## Transcript exon combination :: BC167067.1, FQ214233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTCCTACC CGATGGTATT GCAGTATCGA CCAAGAATTG ATTTCGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123354.1
CDS	303..1013
Translation	MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELQRELEFWKKNKPQENGQDEVSKVDKSKADEEGDENQDDKDYHRSDPQIAICLDCLRNHGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENSYPMVLQYRPRIDFG

Target ORF information:

RefSeq Version	NM_001129882.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129882.1

ORF Insert Sequence:

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTCCTACC CGATGGTATT GCAGTATCGA CCAAGAATTG ATTTCGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf5 ( XM_006231311.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231373.1 Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa02232
Clone ID Related Accession (Same CDS sequence)	XM_006231311.3 , NM_001129882.1
Accession Version	XM_006231311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231311.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTCCTACC CGATGGTATT GCAGTATCGA CCAAGAATTG ATTTCGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231373.1
CDS	393..1103
Translation	MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELQRELEFWKKNKPQENGQDEVSKVDKSKADEEGDENQDDKDYHRSDPQIAICLDCLRNHGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENSYPMVLQYRPRIDFG

Target ORF information:

RefSeq Version	XM_006231311.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231311.3

ORF Insert Sequence:

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTCCTACC CGATGGTATT GCAGTATCGA CCAAGAATTG ATTTCGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf5 ( XM_017589707.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445196.1 Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24048
Clone ID Related Accession (Same CDS sequence)	XM_017589707.1 , XM_017589708.1 , XM_017589709.1
Accession Version	XM_017589707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445196.1
CDS	395..1156
Translation	MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELQRELEFWKKNKPQENGQDEVSKVDKSKADEEGDENQDDKDYHRSDPQIAICLDCLRNHGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENFCCLELFSLASLLSGWHFVSVLPDGIAVSTKN

Target ORF information:

RefSeq Version	XM_017589707.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589707.1

ORF Insert Sequence:

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf5 ( XM_017589708.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445197.1 Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24048
Clone ID Related Accession (Same CDS sequence)	XM_017589707.1 , XM_017589708.1 , XM_017589709.1
Accession Version	XM_017589708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445197.1
CDS	404..1165
Translation	MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELQRELEFWKKNKPQENGQDEVSKVDKSKADEEGDENQDDKDYHRSDPQIAICLDCLRNHGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENFCCLELFSLASLLSGWHFVSVLPDGIAVSTKN

Target ORF information:

RefSeq Version	XM_017589708.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589708.1

ORF Insert Sequence:

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcgf5 ( XM_017589709.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445198.1 Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24048
Clone ID Related Accession (Same CDS sequence)	XM_017589707.1 , XM_017589708.1 , XM_017589709.1
Accession Version	XM_017589709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445198.1
CDS	390..1151
Translation	MATQRKHLVKDFNPYITCYICKGYLIKPTTVTECLHTFCKTCIVQHFEDSNDCPRCGNQVHETNPLEMLRLDNTLEEIIFKLVPGLREQELQRELEFWKKNKPQENGQDEVSKVDKSKADEEGDENQDDKDYHRSDPQIAICLDCLRNHGQSGDNVVKGLMKKFIRCSTRVTVGTIKKFLSLKLKLPSSYELDVLCNGEIMGKDHTMEFIYMTRWRLRGENFCCLELFSLASLLSGWHFVSVLPDGIAVSTKN

Target ORF information:

RefSeq Version	XM_017589709.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 5 (Pcgf5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589709.1

ORF Insert Sequence:

ATGGCTACCC AAAGGAAACA CTTGGTGAAA GATTTCAACC CGTACATCAC CTGCTACATC 
TGTAAAGGAT ATCTGATCAA GCCCACCACG GTGACAGAAT GCCTCCATAC ATTCTGTAAG 
ACCTGTATTG TCCAGCACTT TGAAGACAGC AATGATTGTC CAAGGTGTGG CAACCAAGTT 
CATGAAACAA ATCCCTTAGA AATGTTAAGG CTAGATAACA CGTTGGAGGA GATCATATTT 
AAGCTGGTGC CTGGACTACG AGAGCAAGAA CTTCAGCGTG AGCTGGAATT TTGGAAGAAA 
AATAAACCTC AAGAAAATGG GCAAGATGAG GTTTCGAAGG TTGACAAATC TAAAGCAGAT 
GAAGAAGGTG ATGAAAACCA AGATGATAAG GACTATCACA GAAGTGACCC ACAAATCGCT 
ATCTGCCTGG ACTGTTTACG AAACCATGGG CAGTCAGGGG ACAATGTGGT AAAGGGTCTC 
ATGAAGAAGT TCATTCGCTG TTCTACACGA GTTACTGTAG GAACTATTAA AAAGTTTCTG 
AGTTTAAAAC TAAAACTTCC AAGTTCTTAT GAGTTGGATG TGCTGTGCAA TGGTGAAATT 
ATGGGAAAGG ATCATACTAT GGAATTCATC TATATGACAA GATGGCGGCT AAGAGGAGAA 
AATTTTTGCT GTCTTGAACT GTTCAGCCTC GCCAGTCTGC TCTCAGGATG GCACTTTGTA 
TCAGTCCTAC CCGATGGTAT TGCAGTATCG ACCAAGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Science (New York, N.Y.)356(6342)1081-1084(2017 06)
Almeida M,Pintacuda G,Masui O,Koseki Y,Gdula M,Cerase A,Brown D,Mould A,Innocent C,Nakayama M,Schermelleh L,Nesterova TB,Koseki H,Brockdorff N

An AUTS2-Polycomb complex activates gene expression in the CNS.
Nature516(7531)349-54(2014 Dec)
Gao Z,Lee P,Stafford JM,von Schimmelmann M,Schaefer A,Reinberg D

PRC1 complex diversity: where is it taking us?
Trends in cell biology24(11)632-41(2014 Nov)
Gil J,O'Loghlen A

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Molecular cell49(5)808-24(2013 Mar)
Simon JA,Kingston RE

Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.
Molecular & cellular proteomics : MCP10(4)M110.002642(2011 Apr)
Vandamme J,V?lkel P,Rosnoblet C,Le Faou P,Angrand PO

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Pcgf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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