Spag17 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag17
Entrez Gene ID	690746
Full Name	sperm associated antigen 17
General protein information	Preferred Names sperm associated antigen 17 Names LOW QUALITY PROTEIN: sperm-associated antigen 17 sperm associated antigen 17 sperm-associated antigen 17
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594049.1	XP_017449538.1	sperm-associated antigen 17 isoform X2
XM_008761363.2	XP_008759585.1	LOW QUALITY PROTEIN: sperm-associated antigen 17 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100170502	NP_001123754.1
Homo sapiens (human)	SPAG17	NP_996879.1
Pan troglodytes (chimpanzee)	SPAG17	XP_513687.2
	SPAG17	XP_002801770.1
Canis lupus familiaris (dog)	SPAG17	XP_849615.1
Mus musculus (house mouse)	Spag17	NP_083168.3
Gallus gallus (chicken)	SPAG17	XP_416538.4
Bos taurus (cattle)	SPAG17	NP_001193803.1
Rattus norvegicus (Norway rat)	Spag17	XP_006233092.1
Danio rerio (zebrafish)	si:dkey-208b23.5	XP_689610.5

Gene Ontology

The following Spag17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa50510	XM_008761363.2 Latest version!	Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa54810	XM_017594049.1 Latest version!	Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag17 ( XM_008761363.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759585.1 Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X1, mRNA.

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CloneID	ORa50510
Clone ID Related Accession (Same CDS sequence)	XM_008761363.2
Accession Version	XM_008761363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCACAAGCT ATAAGATATG GGAACCCTCA 
CTCATTGCTG CACATATGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAATTG AGGATGAGCT TCCCATTCAT GCCCTCAACC TGGCTGTCCA ACTGCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGAAA GACATTCTGC AGCAGATCAA TGAAATACAG 
GCCCTTGTTG GAGCTACTAC TAAAAAGGGG AAGAAGACCA TAACTGGCAA CTTACCATTA 
CACTATGAGG TGTTGCTGGC AGCAAAAACA ATCATGGACA ATGGAGAGAA GCTGACCTTA 
CCATTGGTAG GGAAACTCTT GAAATTTCAA CTCCTGCAGA TTAAAACCAA GGACCAGCAG 
AGGCGAGAAA ACGAAAAGAA GATGATAGAA GAAAGAGCAA AGTCGGAGAA GGACAAAGGA 
AAAGGGAAGT CTCCCAAGGA GAAGAAGGCC GCCACTGTTA AGGCTGGGAA GGGAAAGAGC 
AAGGACCAGC CCGAGGCCAC CGTAACAGTG AAGAAGACCA CGCAGTTAAA ACGGAGGGGA 
GAAGATGACG ACACCAAATT CTACATCGAC GATGAGCCGG ACGATGGTGC CCAACATTAC 
GTTATTGTTG TGGGCTTCAA CCATCCTCAG CTATTGGCCA TTATGACTGA ACTTGGCATT 
CCTATTACCA GTGTGATTAA AATATCTTCA GAGAATTATG AACCTCTGAA GACATACCTG 
GCAGCAGTCA TGCAGCAACA GGAAGCTCTC CTTCAGCCGG AAGATATAGC AGCTGAAAAT 
TTGAAGAGAG AAAATGACAT TAAGGAACTT GAGGTCTTCT GGAAGTACTT GGAACCAATC 
CTGAATAATG AGAAGCCTGA AACAAATCTC TTGGATGTTG CTCGACTCCA CTACATGGTC 
AAGACAAAAC ATTTCCCTTC TGACTGGTCA GACTATGAGC AGATGCTTGA CCTAGGCACG 
GAGATCTTTG AAAATGTCGC CTGTTTGATG TACGACAGCC TGGACTGGAA GAGGCAGCAT 
CATCACTATC TGCAAAGCAT GCAGCTCATC AATGTCCCAC AAGTGGTTAG CGATAAGACC 
ATGTTGGAAG CGATCCCGAT TCCAGAGCCT CCACCATCAA CTGCTCCAGC CCCCGGAAAG 
AAGAAAGCAC AGTATGAGGA ATCACATGCT CCCCCATCAG TGGCTTTTAT CATCACAACT 
GAAGTAGACA TGCGGTATTA CAATGACCTG CTGAATTACC AGAAACTTAC AACATTAAGA 
AATTTAGACC ACTGTCAAAT GCAGAAAGTT ATAGCAACGG AAGAGGATCT CACCCCGCCG 
AGTTTGGTGG AGCCAGCCCC CAGAGCAGAT GGGTTAGACT ACAGAATCGC AGCTCACATC 
GTGTCCATCC TGCCTTCCCT CTGCCTCACT GAGAAGGAAA AGAAGAATCT CCACGAGATA 
TTCTTAACTG AAGGAGAGAG CGAAAGCAAA ACCNCCCCCA AAGGCCCTCT CCTGCTGAAC 
TACCACGACA CGTATGCCCA CAAGAAGTAC GCGCTGAAGG ACCAAAAGAA TTTTGACCCG 
GTTCAAATTG AGGAGGAGAT GCAATCCAAG CTGCCACTGT GGGAATTCCT TCAGTTCCCT 
CTGCCGCCTC CGTGGAACAG CACTAAACGG CTTGCCACCA TTCATGAACT GATGCATTTC 
TGTACCAACG AAGTTCTGAG CTGGAATGAA GTAGAACGAG CCTTCAAGGT ATTTACTTTT 
GAAAGCCTGA AGCTCTCAGA GGTGGATGAG GCAGGACGGC TCAAGCCCAC CGAGGTGATC 
AGTGAGACAA ATGTTGAAAA TGTCAACATT CCTTGGGACA ATCCTGCCAG GTTCGCTAAG 
CTGATAAGAC AGCGGTACAT CCACAGGATG AGCATACAGA AGGCCAAGCC AGTATTAGTA 
GAAACCGAAC ACAAAGACAG AACACTATTC GTGAACCAGA ATTTTGCCAA AGCTGAGCAA 
GATGCACAAG GAGATGAAAA CTCCCCAAAT TGTGATCATC CTGCTGCTAA CAATGTGATG 
GGGTCTACTT CCAACTCCAC GAGACCCCGG TATTCTAGTG ATAGACAGCT CACAGAGAAA 
GAGACCAGTG GGTCCATGTG TCCCCAACCA GAGTCTGTGG AGCAGATCAT GGACACTGAG 
ATCAAAGGTG GAGCAGCCAC AAAGGATGAT TCTCTTGAAA AGAAACCCAA GAAGATGGTG 
GTGGAGGCCG ACATAGAGGA CATTAAAAAA ACACAACAGC GCAGTTTAAT GGACTGGAGC 
TTTACTGAAC ATTTTCAGCC GAAAGTCCTG CTTCAGGTTC TTCAGGAGGC TCATCAGCAG 
TACCGATGTG TTGACTCGTA CTACCACACG CAGGACAATT CCTTACTTCT CGTCTTTCAT 
AACCCAATGA ATCTACACCA TCTGCATTGT GAATATTGGA ATATTGCTCT TCATTCCAAC 
GTTGGATTCA GGAATTACTT AGAACTTGTT GCAAAATCTA TTGAAGAATG GGTCATACAG 
GAAGAAGCAA AGTACCAGGA AGCCAAAATG GCTGAGGAGC TCAACAGAGC CAAGAATGAG 
TTGGACTTGA AAGCTACTAC TGCCAAAACT TCTGCTAGCA AAATTGGTGG CCCCAAAAAA 
TCTAAAAGTA ACAAAGTGTC CAGCAAAACA GAGCTAACAG ACCAAGAAAA AGATAAAGAG 
AAAGAGAAAG AAAAAGAGAA AGACAAGATC CCCTTCATAT TAGAAGGCTC TCTCAAGGCA 
TGGAAGGAAG AACAAGAGAG GTTGGCTGAG GAGGAACGCC TGAAGGAGGA AAAGAAGGCA 
GAGAAGAAGG GGAAGGAAGC AGGTAGAAAG AAAGGCAAGG ATAAGGCAGA CAAAGAAGAC 
ACTAAGAGTT TGAAAAAAAA GTCGTCTTCC AAGGAGAAAC CTAAGGAAGA ACCATCTAAG 
TCGCTGGAAG CAATAGAGGA AGCCGTCCCA CTTCCAGAAC CGGAAATTGT TTACCCGTTC 
CGAGGATACA ATATGGGAGA CATTCCTATC CAAATCTCAG GGACAAATTA CTATCTGTAT 
CCTTCTGACG GTGGGCAGAT TGAAGTGGAA AAGATCAGGT TTGAACGAGG CCCAACTTTC 
ATCAAAGTGA AAGTAAAAAA AGATAAACAC AGTTTTATAA TTCACCTGAA AGATCCAAAG 
GAAATCGTGA AAAGGGAAAA GGAAGAGGAG CACTCAGAAG AAGAGGAAGA GGAAGAGGAG 
GAGGAAGAGG AAGAGGAAGA GGAAGAGAAT GAACAGAAGG AAGGGAAAGA GGAGAATGTT 
AAACAAAATG TGGAAATGAA GAGAACACAG ATAAAGAAGG AAGCTGTCAG CAAGTTTGGA 
TCCTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCCA TAAGTTACTA TGGATCAAAT 
GGAATGGCGC CAGAGGTTAT GGATTCTGAT TTGGAAGCAA TGATGAATAT CCCTTCAGCT 
ATGACAGCAA CAGTGGTTCC CTCTATAGTT ACTGTTCCCC AAGGCAAAGG CAAAACTAAG 
CCGAAGGGCA AAGAAAAACA CAAAGATTCC TTTAAAGAAG AGGAACTTCC AAAAGAAGAA 
GAAAAGAAGA ATCACTCACA GGAAGAAGTA GAACCAGAGA TTGTTATACA GGAAACACCT 
TATGTGCCCA CCTTCCAAAA CCTAAATGTA TCTTGCCCCA ATGGGCTCCT GCTTACCTTC 
ATTGGGCAAG AATCTACAGA TGAAGAATCT ACCTGGAACC TCATGATCCG CCAGAGCTAC 
CCCCAGAGGT TGAAGCACTA TGAATTCTAT AAAGCAGTGA TGCCACCCAC AGAGCAGGAG 
GCATCAAGAG TTGTCACCAG CCAGGGCACC GTTATCAAGT ATATGCTGGA TGGATCCACA 
CAGATTCTTT TTGCAGATGG TGCTGTGAGC AGCAGTCCTG ACTCAGGTCC TGTTCATGCA 
TCTCCTGAGA CGTCTGCAAG CCCCCACAAT GGAGATGTGG CAGACAACAC TTCTCAGCAT 
AAATCCGAAA CAGCACCTTC TGAGATCATC ACCAAGAAAG GAAAAGGTCA CAAAAATCAA 
ACATCAACGG CAAGTAAGAG CGAAGCCCAT GATACTCTCC CAGAAGTGGT TCCAACTCCT 
ATGGAGTCCC ACACTGGCAC CTGGTTTACA ACAACGCCTG ATGGAAGGAG GATCGGCACC 
AAAGGCCTAG AGAAAATCAC CAACTTGAAA CCATTTTTAT TCTTCCAGGC TACAGATCCC 
ATCAATGGAA CGGTCATGAC TACTCGAGAA GACAAGGTGA TCATTGTTGA GAAGAAGGAC 
GGGACCCGGA TCGTGGACCA TGCCGATGGT ACCAGAATCA CAACCTTTTA CCAAGTGTAT 
GAAGATCATA TTACTCCCCC AAATGATGAA GAAACAACCG AAGATCCAAG GACTGTCACT 
AGACAGGTGA AGTGCATGCG GATAGAAAGC TCGCACTATG CCACTATTAT CACGAACTGC 
GAGGACAGTA GCTGCTGTGC CACTTTTGGG GACGGAACAT CTATTATTGC AAAGCCACAG 
GGATCATACC AGGTGCTACC TTCAAACACA GGCTGCCTTT ATATCGACAA AGACTGCTCG 
GCCACATATT GCCATGAATT GAGCAGTAAT ATGTACCATC CCTTCCAGAA GCGTGAAGAG 
CTGAGAGCCA GCAGGTACAT CATGAAGCAC ACCTCAGAGG TGATCTGTGA GGTCCAGGAT 
CCCGAGGGGA ACATCTTTAA GGTCATGGCT GATGGCTCTG TATCAACAGC GTTACCTAAA 
AAGAAGCTAG AAGACAAGTT GAATGTACAG ACGGAGGGCT ACGAAAGCTT ATCATCACTT 
CACCTTGAAA AGAATCGCAT GCAGATCTAT GGTGAACATG TTCCCAGATT TTTTGTTGTC 
TATACTGATG GATCAGGAGT GGAACTTCTC CGAGACAGTG ACATAGAAGA ATACTTATCC 
TTGGCCTATG GAGAGTCAAC GACTGTGGTT CTCCAGGAAC CAGTACAGGA ACACCCAGGT 
GCCCTGAGCA TCACAGTCCT TCGCCCTTTC CATGAGGCAT CACAATGGAT CATGAAGAAG 
GAGCTGGACA GCATCGTTCC TCCAAATCTC CAGTCGAGAT CATGGGAGAC ATTTCCTTCA 
GTTGAGAAAA AGACTCCAGG ACCACCATTT GGCACTCAGA TTTGGAAAGG CCTCTGTATT 
GGGGCTAAGC AGCTGACAAA CATCCCAGCT CCTATACTTG AAGGCCCCAA GGTGCTACAG 
ATGCGTCAGT TCATCCAGCA CGAAGTCATA AAGAACGAGG TGAAACTGAA GCTGCAAATT 
TCCCTGAAGG ACTATATAAA CCACATCCTG AAGAAAGAAG ATGAGCAACA AGAGATGACA 
GTCAAGGATT CCAGAACGGA GGAGGAGAGA GGCAATGCTG CAGATCTCCT CAAGCTGGTT 
ATGGTGAGAG GGTGTCCCAC AGTCCAGTGT TCTTGCTACT GTCTCACTGT CAGGTGCTCC 
CATGCAGACT GTATATGGGG AAAGCTACAT GAGATTCCTA CCAAGGCTTT GAAACATTTG 
CTTCATCCAC ATCATGCCTT ATTTCTACTA CTGCCCGCTA CAGCTTGGGT ACAGAAATCC 
TCCAAATCAA CTGTTGCTGC CCACCTAACA GATTTATTCA AGCAATCTAT GGCTTCGGCT 
CCAAAATGTT CACCAGAAAA CTTCACTAAA GATTTCTTTG AAAAGAAGTG GAGGGAGGCT 
CCACAAGGAA AGAGTTGGAA AGAAAAGCTT GAGCAACAGA GGAACAATAT TAAGAAAACA 
CAAAATTACC TAATGCAAAT TAGGACAAAA GAAGTAACGC CTTATTTTAA GTCTGAATTG 
AGCCCATTGA TTAAGTCACA GTATGATTAT CTGGAGAAGC TTTCCAAAAA CCTGCCTCCG 
TTTACAAAGA AAAATGAGGC TGAAACCAAG ACAGCTGACC CAGATCTTCA CTCAGATCAC 
ATCCCCTCAC AACAAAGAGA AAGTGTTAAC AATGAGAATA GCCAGGAATC TACTGGCCAA 
AAGGAAACTG AATATGTAAC CAAATATCTC CAGACCTCTT CTCCCCCAAA CCAATATGAA 
CATTTAACAA AATCTCCCAA GGAGTCATAT AGTCAAAGTC AAAGTGAAAT TGAAACCAAC 
GCTACCAAGG AACCTGCTGG CCAGAACCAC ACTGAAAAGT TTAAAATTTC TACCAAGAAG 
TCTGCTAGCC AAAACCAAAT TGAAAATCTA ATAAAATCTA CCAAGGAGCC TGCTGGTCAA 
AGCCAAATGG AAAATATCTC CAGACCGCCT ACTGAAGAAC CAGAAAATTT TATGCCAATC 
AAAATTCCAA CCCAGTCATT GCTGCAGGAT GTTACTGGAA AAGCAAGAAA AGAGAAAGTG 
AGGTTACCTC ATTACATGAT GAGCTGCAAG CCCAAGTGTC AGCCTCACAC CAAGGTGGAA 
GATTCTGTTG GAGGAAAAGT GAACACATCC TCCATTGCAT GTGCTGCTAT TTATAACCCA 
GACGTATCCC CTTTCGGGTT CCACCTTCTC CCACCATCAA TACAGTTTGG AGTGCTTAAA 
GAAGGACACA CCTATGCAAC GATTGTGAAG CTCAAGAATG TTGGTGTGGA CTTCTGCAGG 
TTTAGAGTGA AGCAGCCCCC ACCCAGCACG GGACTGAAAG TGACATATAA ACCTGGGCCT 
GTGGCTGCTG GCTTGCAAGC AGAGCTAAAG GTAGAGCTAT TTGCCATGGC TGTTGGAGAA 
GACGGCGCCA AGGACTCAGC ACACATCTCC CACACCATTG AAATCCTGAC GGAGCACGAT 
GTTCTATTCT TACCCGTGGA GGCAACAGTT TTAACAAGCA GTAACTATGA CAATCGACCA 
AAAGATCTTC CCCAGGGAAG AGAAAATCCC ATGGTCTACA GGACTTCCAC AATCTCCTCC 
TCTGCACTTG GAGTCGTCAT GTCTCAGAAG GCCACTCGTC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759585.1
CDS	140..7204
Translation	MAPKKEKPTGSTSYKIWEPSLIAAHMNQNDWKASIAFVVGNRIEDELPIHALNLAVQLPQRKLFSIVSWKDILQQINEIQALVGATTKKGKKTITGNLPLHYEVLLAAKTIMDNGEKLTLPLVGKLLKFQLLQIKTKDQQRRENEKKMIEERAKSEKDKGKGKSPKEKKAATVKAGKGKSKDQPEATVTVKKTTQLKRRGEDDDTKFYIDDEPDDGAQHYVIVVGFNHPQLLAIMTELGIPITSVIKISSENYEPLKTYLAAVMQQQEALLQPEDIAAENLKRENDIKELEVFWKYLEPILNNEKPETNLLDVARLHYMVKTKHFPSDWSDYEQMLDLGTEIFENVACLMYDSLDWKRQHHHYLQSMQLINVPQVVSDKTMLEAIPIPEPPPSTAPAPGKKKAQYEESHAPPSVAFIITTEVDMRYYNDLLNYQKLTTLRNLDHCQMQKVIATEEDLTPPSLVEPAPRADGLDYRIAAHIVSILPSLCLTEKEKKNLHEIFLTEGESESKTnullPKGPLLLNYHDTYAHKKYALKDQKNFDPVQIEEEMQSKLPLWEFLQFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSEVDEAGRLKPTEVISETNVENVNIPWDNPARFAKLIRQRYIHRMSIQKAKPVLVETEHKDRTLFVNQNFAKAEQDAQGDENSPNCDHPAANNVMGSTSNSTRPRYSSDRQLTEKETSGSMCPQPESVEQIMDTEIKGGAATKDDSLEKKPKKMVVEADIEDIKKTQQRSLMDWSFTEHFQPKVLLQVLQEAHQQYRCVDSYYHTQDNSLLLVFHNPMNLHHLHCEYWNIALHSNVGFRNYLELVAKSIEEWVIQEEAKYQEAKMAEELNRAKNELDLKATTAKTSASKIGGPKKSKSNKVSSKTELTDQEKDKEKEKEKEKDKIPFILEGSLKAWKEEQERLAEEERLKEEKKAEKKGKEAGRKKGKDKADKEDTKSLKKKSSSKEKPKEEPSKSLEAIEEAVPLPEPEIVYPFRGYNMGDIPIQISGTNYYLYPSDGGQIEVEKIRFERGPTFIKVKVKKDKHSFIIHLKDPKEIVKREKEEEHSEEEEEEEEEEEEEEEEENEQKEGKEENVKQNVEMKRTQIKKEAVSKFGSFSATLENGICLSISYYGSNGMAPEVMDSDLEAMMNIPSAMTATVVPSIVTVPQGKGKTKPKGKEKHKDSFKEEELPKEEEKKNHSQEEVEPEIVIQETPYVPTFQNLNVSCPNGLLLTFIGQESTDEESTWNLMIRQSYPQRLKHYEFYKAVMPPTEQEASRVVTSQGTVIKYMLDGSTQILFADGAVSSSPDSGPVHASPETSASPHNGDVADNTSQHKSETAPSEIITKKGKGHKNQTSTASKSEAHDTLPEVVPTPMESHTGTWFTTTPDGRRIGTKGLEKITNLKPFLFFQATDPINGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHITPPNDEETTEDPRTVTRQVKCMRIESSHYATIITNCEDSSCCATFGDGTSIIAKPQGSYQVLPSNTGCLYIDKDCSATYCHELSSNMYHPFQKREELRASRYIMKHTSEVICEVQDPEGNIFKVMADGSVSTALPKKKLEDKLNVQTEGYESLSSLHLEKNRMQIYGEHVPRFFVVYTDGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEHPGALSITVLRPFHEASQWIMKKELDSIVPPNLQSRSWETFPSVEKKTPGPPFGTQIWKGLCIGAKQLTNIPAPILEGPKVLQMRQFIQHEVIKNEVKLKLQISLKDYINHILKKEDEQQEMTVKDSRTEEERGNAADLLKLVMVRGCPTVQCSCYCLTVRCSHADCIWGKLHEIPTKALKHLLHPHHALFLLLPATAWVQKSSKSTVAAHLTDLFKQSMASAPKCSPENFTKDFFEKKWREAPQGKSWKEKLEQQRNNIKKTQNYLMQIRTKEVTPYFKSELSPLIKSQYDYLEKLSKNLPPFTKKNEAETKTADPDLHSDHIPSQQRESVNNENSQESTGQKETEYVTKYLQTSSPPNQYEHLTKSPKESYSQSQSEIETNATKEPAGQNHTEKFKISTKKSASQNQIENLIKSTKEPAGQSQMENISRPPTEEPENFMPIKIPTQSLLQDVTGKARKEKVRLPHYMMSCKPKCQPHTKVEDSVGGKVNTSSIACAAIYNPDVSPFGFHLLPPSIQFGVLKEGHTYATIVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFAMAVGEDGAKDSAHISHTIEILTEHDVLFLPVEATVLTSSNYDNRPKDLPQGRENPMVYRTSTISSSALGVVMSQKATRH

Target ORF information:

RefSeq Version	XM_008761363.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761363.2

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCACAAGCT ATAAGATATG GGAACCCTCA 
CTCATTGCTG CACATATGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAATTG AGGATGAGCT TCCCATTCAT GCCCTCAACC TGGCTGTCCA ACTGCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGAAA GACATTCTGC AGCAGATCAA TGAAATACAG 
GCCCTTGTTG GAGCTACTAC TAAAAAGGGG AAGAAGACCA TAACTGGCAA CTTACCATTA 
CACTATGAGG TGTTGCTGGC AGCAAAAACA ATCATGGACA ATGGAGAGAA GCTGACCTTA 
CCATTGGTAG GGAAACTCTT GAAATTTCAA CTCCTGCAGA TTAAAACCAA GGACCAGCAG 
AGGCGAGAAA ACGAAAAGAA GATGATAGAA GAAAGAGCAA AGTCGGAGAA GGACAAAGGA 
AAAGGGAAGT CTCCCAAGGA GAAGAAGGCC GCCACTGTTA AGGCTGGGAA GGGAAAGAGC 
AAGGACCAGC CCGAGGCCAC CGTAACAGTG AAGAAGACCA CGCAGTTAAA ACGGAGGGGA 
GAAGATGACG ACACCAAATT CTACATCGAC GATGAGCCGG ACGATGGTGC CCAACATTAC 
GTTATTGTTG TGGGCTTCAA CCATCCTCAG CTATTGGCCA TTATGACTGA ACTTGGCATT 
CCTATTACCA GTGTGATTAA AATATCTTCA GAGAATTATG AACCTCTGAA GACATACCTG 
GCAGCAGTCA TGCAGCAACA GGAAGCTCTC CTTCAGCCGG AAGATATAGC AGCTGAAAAT 
TTGAAGAGAG AAAATGACAT TAAGGAACTT GAGGTCTTCT GGAAGTACTT GGAACCAATC 
CTGAATAATG AGAAGCCTGA AACAAATCTC TTGGATGTTG CTCGACTCCA CTACATGGTC 
AAGACAAAAC ATTTCCCTTC TGACTGGTCA GACTATGAGC AGATGCTTGA CCTAGGCACG 
GAGATCTTTG AAAATGTCGC CTGTTTGATG TACGACAGCC TGGACTGGAA GAGGCAGCAT 
CATCACTATC TGCAAAGCAT GCAGCTCATC AATGTCCCAC AAGTGGTTAG CGATAAGACC 
ATGTTGGAAG CGATCCCGAT TCCAGAGCCT CCACCATCAA CTGCTCCAGC CCCCGGAAAG 
AAGAAAGCAC AGTATGAGGA ATCACATGCT CCCCCATCAG TGGCTTTTAT CATCACAACT 
GAAGTAGACA TGCGGTATTA CAATGACCTG CTGAATTACC AGAAACTTAC AACATTAAGA 
AATTTAGACC ACTGTCAAAT GCAGAAAGTT ATAGCAACGG AAGAGGATCT CACCCCGCCG 
AGTTTGGTGG AGCCAGCCCC CAGAGCAGAT GGGTTAGACT ACAGAATCGC AGCTCACATC 
GTGTCCATCC TGCCTTCCCT CTGCCTCACT GAGAAGGAAA AGAAGAATCT CCACGAGATA 
TTCTTAACTG AAGGAGAGAG CGAAAGCAAA ACCNCCCCCA AAGGCCCTCT CCTGCTGAAC 
TACCACGACA CGTATGCCCA CAAGAAGTAC GCGCTGAAGG ACCAAAAGAA TTTTGACCCG 
GTTCAAATTG AGGAGGAGAT GCAATCCAAG CTGCCACTGT GGGAATTCCT TCAGTTCCCT 
CTGCCGCCTC CGTGGAACAG CACTAAACGG CTTGCCACCA TTCATGAACT GATGCATTTC 
TGTACCAACG AAGTTCTGAG CTGGAATGAA GTAGAACGAG CCTTCAAGGT ATTTACTTTT 
GAAAGCCTGA AGCTCTCAGA GGTGGATGAG GCAGGACGGC TCAAGCCCAC CGAGGTGATC 
AGTGAGACAA ATGTTGAAAA TGTCAACATT CCTTGGGACA ATCCTGCCAG GTTCGCTAAG 
CTGATAAGAC AGCGGTACAT CCACAGGATG AGCATACAGA AGGCCAAGCC AGTATTAGTA 
GAAACCGAAC ACAAAGACAG AACACTATTC GTGAACCAGA ATTTTGCCAA AGCTGAGCAA 
GATGCACAAG GAGATGAAAA CTCCCCAAAT TGTGATCATC CTGCTGCTAA CAATGTGATG 
GGGTCTACTT CCAACTCCAC GAGACCCCGG TATTCTAGTG ATAGACAGCT CACAGAGAAA 
GAGACCAGTG GGTCCATGTG TCCCCAACCA GAGTCTGTGG AGCAGATCAT GGACACTGAG 
ATCAAAGGTG GAGCAGCCAC AAAGGATGAT TCTCTTGAAA AGAAACCCAA GAAGATGGTG 
GTGGAGGCCG ACATAGAGGA CATTAAAAAA ACACAACAGC GCAGTTTAAT GGACTGGAGC 
TTTACTGAAC ATTTTCAGCC GAAAGTCCTG CTTCAGGTTC TTCAGGAGGC TCATCAGCAG 
TACCGATGTG TTGACTCGTA CTACCACACG CAGGACAATT CCTTACTTCT CGTCTTTCAT 
AACCCAATGA ATCTACACCA TCTGCATTGT GAATATTGGA ATATTGCTCT TCATTCCAAC 
GTTGGATTCA GGAATTACTT AGAACTTGTT GCAAAATCTA TTGAAGAATG GGTCATACAG 
GAAGAAGCAA AGTACCAGGA AGCCAAAATG GCTGAGGAGC TCAACAGAGC CAAGAATGAG 
TTGGACTTGA AAGCTACTAC TGCCAAAACT TCTGCTAGCA AAATTGGTGG CCCCAAAAAA 
TCTAAAAGTA ACAAAGTGTC CAGCAAAACA GAGCTAACAG ACCAAGAAAA AGATAAAGAG 
AAAGAGAAAG AAAAAGAGAA AGACAAGATC CCCTTCATAT TAGAAGGCTC TCTCAAGGCA 
TGGAAGGAAG AACAAGAGAG GTTGGCTGAG GAGGAACGCC TGAAGGAGGA AAAGAAGGCA 
GAGAAGAAGG GGAAGGAAGC AGGTAGAAAG AAAGGCAAGG ATAAGGCAGA CAAAGAAGAC 
ACTAAGAGTT TGAAAAAAAA GTCGTCTTCC AAGGAGAAAC CTAAGGAAGA ACCATCTAAG 
TCGCTGGAAG CAATAGAGGA AGCCGTCCCA CTTCCAGAAC CGGAAATTGT TTACCCGTTC 
CGAGGATACA ATATGGGAGA CATTCCTATC CAAATCTCAG GGACAAATTA CTATCTGTAT 
CCTTCTGACG GTGGGCAGAT TGAAGTGGAA AAGATCAGGT TTGAACGAGG CCCAACTTTC 
ATCAAAGTGA AAGTAAAAAA AGATAAACAC AGTTTTATAA TTCACCTGAA AGATCCAAAG 
GAAATCGTGA AAAGGGAAAA GGAAGAGGAG CACTCAGAAG AAGAGGAAGA GGAAGAGGAG 
GAGGAAGAGG AAGAGGAAGA GGAAGAGAAT GAACAGAAGG AAGGGAAAGA GGAGAATGTT 
AAACAAAATG TGGAAATGAA GAGAACACAG ATAAAGAAGG AAGCTGTCAG CAAGTTTGGA 
TCCTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCCA TAAGTTACTA TGGATCAAAT 
GGAATGGCGC CAGAGGTTAT GGATTCTGAT TTGGAAGCAA TGATGAATAT CCCTTCAGCT 
ATGACAGCAA CAGTGGTTCC CTCTATAGTT ACTGTTCCCC AAGGCAAAGG CAAAACTAAG 
CCGAAGGGCA AAGAAAAACA CAAAGATTCC TTTAAAGAAG AGGAACTTCC AAAAGAAGAA 
GAAAAGAAGA ATCACTCACA GGAAGAAGTA GAACCAGAGA TTGTTATACA GGAAACACCT 
TATGTGCCCA CCTTCCAAAA CCTAAATGTA TCTTGCCCCA ATGGGCTCCT GCTTACCTTC 
ATTGGGCAAG AATCTACAGA TGAAGAATCT ACCTGGAACC TCATGATCCG CCAGAGCTAC 
CCCCAGAGGT TGAAGCACTA TGAATTCTAT AAAGCAGTGA TGCCACCCAC AGAGCAGGAG 
GCATCAAGAG TTGTCACCAG CCAGGGCACC GTTATCAAGT ATATGCTGGA TGGATCCACA 
CAGATTCTTT TTGCAGATGG TGCTGTGAGC AGCAGTCCTG ACTCAGGTCC TGTTCATGCA 
TCTCCTGAGA CGTCTGCAAG CCCCCACAAT GGAGATGTGG CAGACAACAC TTCTCAGCAT 
AAATCCGAAA CAGCACCTTC TGAGATCATC ACCAAGAAAG GAAAAGGTCA CAAAAATCAA 
ACATCAACGG CAAGTAAGAG CGAAGCCCAT GATACTCTCC CAGAAGTGGT TCCAACTCCT 
ATGGAGTCCC ACACTGGCAC CTGGTTTACA ACAACGCCTG ATGGAAGGAG GATCGGCACC 
AAAGGCCTAG AGAAAATCAC CAACTTGAAA CCATTTTTAT TCTTCCAGGC TACAGATCCC 
ATCAATGGAA CGGTCATGAC TACTCGAGAA GACAAGGTGA TCATTGTTGA GAAGAAGGAC 
GGGACCCGGA TCGTGGACCA TGCCGATGGT ACCAGAATCA CAACCTTTTA CCAAGTGTAT 
GAAGATCATA TTACTCCCCC AAATGATGAA GAAACAACCG AAGATCCAAG GACTGTCACT 
AGACAGGTGA AGTGCATGCG GATAGAAAGC TCGCACTATG CCACTATTAT CACGAACTGC 
GAGGACAGTA GCTGCTGTGC CACTTTTGGG GACGGAACAT CTATTATTGC AAAGCCACAG 
GGATCATACC AGGTGCTACC TTCAAACACA GGCTGCCTTT ATATCGACAA AGACTGCTCG 
GCCACATATT GCCATGAATT GAGCAGTAAT ATGTACCATC CCTTCCAGAA GCGTGAAGAG 
CTGAGAGCCA GCAGGTACAT CATGAAGCAC ACCTCAGAGG TGATCTGTGA GGTCCAGGAT 
CCCGAGGGGA ACATCTTTAA GGTCATGGCT GATGGCTCTG TATCAACAGC GTTACCTAAA 
AAGAAGCTAG AAGACAAGTT GAATGTACAG ACGGAGGGCT ACGAAAGCTT ATCATCACTT 
CACCTTGAAA AGAATCGCAT GCAGATCTAT GGTGAACATG TTCCCAGATT TTTTGTTGTC 
TATACTGATG GATCAGGAGT GGAACTTCTC CGAGACAGTG ACATAGAAGA ATACTTATCC 
TTGGCCTATG GAGAGTCAAC GACTGTGGTT CTCCAGGAAC CAGTACAGGA ACACCCAGGT 
GCCCTGAGCA TCACAGTCCT TCGCCCTTTC CATGAGGCAT CACAATGGAT CATGAAGAAG 
GAGCTGGACA GCATCGTTCC TCCAAATCTC CAGTCGAGAT CATGGGAGAC ATTTCCTTCA 
GTTGAGAAAA AGACTCCAGG ACCACCATTT GGCACTCAGA TTTGGAAAGG CCTCTGTATT 
GGGGCTAAGC AGCTGACAAA CATCCCAGCT CCTATACTTG AAGGCCCCAA GGTGCTACAG 
ATGCGTCAGT TCATCCAGCA CGAAGTCATA AAGAACGAGG TGAAACTGAA GCTGCAAATT 
TCCCTGAAGG ACTATATAAA CCACATCCTG AAGAAAGAAG ATGAGCAACA AGAGATGACA 
GTCAAGGATT CCAGAACGGA GGAGGAGAGA GGCAATGCTG CAGATCTCCT CAAGCTGGTT 
ATGGTGAGAG GGTGTCCCAC AGTCCAGTGT TCTTGCTACT GTCTCACTGT CAGGTGCTCC 
CATGCAGACT GTATATGGGG AAAGCTACAT GAGATTCCTA CCAAGGCTTT GAAACATTTG 
CTTCATCCAC ATCATGCCTT ATTTCTACTA CTGCCCGCTA CAGCTTGGGT ACAGAAATCC 
TCCAAATCAA CTGTTGCTGC CCACCTAACA GATTTATTCA AGCAATCTAT GGCTTCGGCT 
CCAAAATGTT CACCAGAAAA CTTCACTAAA GATTTCTTTG AAAAGAAGTG GAGGGAGGCT 
CCACAAGGAA AGAGTTGGAA AGAAAAGCTT GAGCAACAGA GGAACAATAT TAAGAAAACA 
CAAAATTACC TAATGCAAAT TAGGACAAAA GAAGTAACGC CTTATTTTAA GTCTGAATTG 
AGCCCATTGA TTAAGTCACA GTATGATTAT CTGGAGAAGC TTTCCAAAAA CCTGCCTCCG 
TTTACAAAGA AAAATGAGGC TGAAACCAAG ACAGCTGACC CAGATCTTCA CTCAGATCAC 
ATCCCCTCAC AACAAAGAGA AAGTGTTAAC AATGAGAATA GCCAGGAATC TACTGGCCAA 
AAGGAAACTG AATATGTAAC CAAATATCTC CAGACCTCTT CTCCCCCAAA CCAATATGAA 
CATTTAACAA AATCTCCCAA GGAGTCATAT AGTCAAAGTC AAAGTGAAAT TGAAACCAAC 
GCTACCAAGG AACCTGCTGG CCAGAACCAC ACTGAAAAGT TTAAAATTTC TACCAAGAAG 
TCTGCTAGCC AAAACCAAAT TGAAAATCTA ATAAAATCTA CCAAGGAGCC TGCTGGTCAA 
AGCCAAATGG AAAATATCTC CAGACCGCCT ACTGAAGAAC CAGAAAATTT TATGCCAATC 
AAAATTCCAA CCCAGTCATT GCTGCAGGAT GTTACTGGAA AAGCAAGAAA AGAGAAAGTG 
AGGTTACCTC ATTACATGAT GAGCTGCAAG CCCAAGTGTC AGCCTCACAC CAAGGTGGAA 
GATTCTGTTG GAGGAAAAGT GAACACATCC TCCATTGCAT GTGCTGCTAT TTATAACCCA 
GACGTATCCC CTTTCGGGTT CCACCTTCTC CCACCATCAA TACAGTTTGG AGTGCTTAAA 
GAAGGACACA CCTATGCAAC GATTGTGAAG CTCAAGAATG TTGGTGTGGA CTTCTGCAGG 
TTTAGAGTGA AGCAGCCCCC ACCCAGCACG GGACTGAAAG TGACATATAA ACCTGGGCCT 
GTGGCTGCTG GCTTGCAAGC AGAGCTAAAG GTAGAGCTAT TTGCCATGGC TGTTGGAGAA 
GACGGCGCCA AGGACTCAGC ACACATCTCC CACACCATTG AAATCCTGAC GGAGCACGAT 
GTTCTATTCT TACCCGTGGA GGCAACAGTT TTAACAAGCA GTAACTATGA CAATCGACCA 
AAAGATCTTC CCCAGGGAAG AGAAAATCCC ATGGTCTACA GGACTTCCAC AATCTCCTCC 
TCTGCACTTG GAGTCGTCAT GTCTCAGAAG GCCACTCGTC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag17 ( XM_017594049.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449538.1 Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X2, mRNA.

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CloneID	ORa54810
Clone ID Related Accession (Same CDS sequence)	XM_017594049.1
Accession Version	XM_017594049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCACAAGCT ATAAGATATG GGAACCCTCA 
CTCATTGCTG CACATATGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAATTG AGGATGAGCT TCCCATTCAT GCCCTCAACC TGGCTGTCCA ACTGCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGAAA GACATTCTGC AGCAGATCAA TGAAATACAG 
GCCCTTGTTG GAGCTACTAC TAAAAAGGGG AAGAAGACCA TAACTGGCAA CTTACCATTA 
CACTATGAGG TGTTGCTGGC AGCAAAAACA ATCATGGACA ATGGAGAGAA GCTGACCTTA 
CCATTGGTAG GGAAACTCTT GAAATTTCAA CTCCTGCAGA TTAAAACCAA GGACCAGCAG 
AGGCGAGAAA ACGAAAAGAA GATGATAGAA GAAAGAGCAA AGTCGGAGAA GGACAAAGGA 
AAAGGGAAGT CTCCCAAGGA GAAGAAGGCC GCCACTGTTA AGGCTGGGAA GGGAAAGAGC 
AAGGACCAGC CCGAGGCCAC CGTAACAGTG AAGAAGACCA CGCAGTTAAA ACGGAGGGGA 
GAAGATGACG ACACCAAATT CTACATCGAC GATGAGCCGG ACGATGGTGC CCAACATTAC 
GTTATTGTTG TGGGCTTCAA CCATCCTCAG CTATTGGCCA TTATGACTGA ACTTGGCATT 
CCTATTACCA GTGTGATTAA AATATCTTCA GAGAATTATG AACCTCTGAA GACATACCTG 
GCAGCAGTCA TGCAGCAACA GGAAGCTCTC CTTCAGCCGG AAGATATAGC AGCTGAAAAT 
TTGAAGAGAG AAAATGACAT TAAGGAACTT GAGGTCTTCT GGAAGTACTT GGAACCAATC 
CTGAATAATG AGAAGCCTGA AACAAATCTC TTGGATGTTG CTCGACTCCA CTACATGGTC 
AAGACAAAAC ATTTCCCTTC TGACTGGTCA GACTATGAGC AGATGCTTGA CCTAGGCACG 
GAGATCTTTG AAAATGTCGC CTGTTTGATG TACGACAGCC TGGACTGGAA GAGGCAGCAT 
CATCACTATC TGCAAAGCAT GCAGCTCATC AATGTCCCAC AAGTGGTTAG CGATAAGACC 
ATGTTGGAAG CGATCCCGAT TCCAGAGCCT CCACCATCAA CTGCTCCAGC CCCCGGAAAG 
AAGAAAGCAC AGTATGAGGA ATCACATGCT CCCCCATCAG TGGCTTTTAT CATCACAACT 
GAAGTAGACA TGCGGTATTA CAATGACCTG CTGAATCCAA TTCCAGAGGA ATTTATTTCA 
GTGCCCCTGA TACTGCACTG TATGCTGGAA CAGGTTATAG CAACGGAAGA GGATCTCACC 
CCGCCGAGTT TGGTGGAGCC AGCCCCCAGA GCAGATGGGT TAGACTACAG AATCGCAGCT 
CACATCGTGT CCATCCTGCC TTCCCTCTGC CTCACTGAGA AGGAAAAGAA GAATCTCCAC 
GAGATATTCT TAACTGAAGG AGAGAGCGAA AGCAAAACCC CACCCAAAGG CCCTCTCCTG 
CTGAACTACC ACGACACGTA TGCCCACAAG AAGTACGCGC TGAAGGACCA AAAGAATTTT 
GACCCGGTTC AAATTGAGGA GGAGATGCAA TCCAAGCTGC CACTGTGGGA ATTCCTTCAG 
TTCCCTCTGC CGCCTCCGTG GAACAGCACT AAACGGCTTG CCACCATTCA TGAACTGATG 
CATTTCTGTA CCAACGAAGT TCTGAGCTGG AATGAAGTAG AACGAGCCTT CAAGGTATTT 
ACTTTTGAAA GCCTGAAGCT CTCAGAGGTG GATGAGGCAG GACGGCTCAA GCCCACCGAG 
GTGATCAGTG AGACAAATGT TGAAAATGTC AACATTCCTT GGGACAATCC TGCCAGGTTC 
GCTAAGCTGA TAAGACAGCG GTACATCCAC AGGATGAGCA TACAGAAGGC CAAGCCAGTA 
TTAGTAGAAA CCGAACACAA AGACAGAACA CTATTCGTGA ACCAGAATTT TGCCAAAGCT 
GAGCAAGATG CACAAGGAGA TGAAAACTCC CCAAATTGTG ATCATCCTGC TGCTAACAAT 
GTGATGGGGT CTACTTCCAA CTCCACGAGA CCCCGGTATT CTAGTGATAG ACAGCTCACA 
GAGAAAGAGA CCAGTGGGTC CATGTGTCCC CAACCAGAGT CTGTGGAGCA GATCATGGAC 
ACTGAGATCA AAGGTGGAGC AGCCACAAAG GATGATTCTC TTGAAAAGAA ACCCAAGAAG 
ATGGTGGTGG AGGCCGACAT AGAGGACATT AAAAAAACAC AACAGCGCAG TTTAATGGAC 
TGGAGCTTTA CTGAACATTT TCAGCCGAAA GTCCTGCTTC AGGTTCTTCA GGAGGCTCAT 
CAGCAGTACC GATGTGTTGA CTCGTACTAC CACACGCAGG ACAATTCCTT ACTTCTCGTC 
TTTCATAACC CAATGAATCT ACACCATCTG CATTGTGAAT ATTGGAATAT TGCTCTTCAT 
TCCAACGTTG GATTCAGGAA TTACTTAGAA CTTGTTGCAA AATCTATTGA AGAATGGGTC 
ATACAGGAAG AAGCAAAGTA CCAGGAAGCC AAAATGGCTG AGGAGCTCAA CAGAGCCAAG 
AATGAGTTGG ACTTGAAAGC TACTACTGCC AAAACTTCTG CTAGCAAAAT TGGTGGCCCC 
AAAAAATCTA AAAGTAACAA AGTGTCCAGC AAAACAGAGC TAACAGACCA AGAAAAAGAT 
AAAGAGAAAG AGAAAGAAAA AGAGAAAGAC AAGATCCCCT TCATATTAGA AGGCTCTCTC 
AAGGCATGGA AGGAAGAACA AGAGAGGTTG GCTGAGGAGG AACGCCTGAA GGAGGAAAAG 
AAGGCAGAGA AGAAGGGGAA GGAAGCAGGT AGAAAGAAAG GCAAGGATAA GGCAGACAAA 
GAAGACACTA AGAGTTTGAA AAAAAAGTCG TCTTCCAAGG AGAAACCTAA GGAAGAACCA 
TCTAAGTCGC TGGAAGCAAT AGAGGAAGCC GTCCCACTTC CAGAACCGGA AATTGTTTAC 
CCGTTCCGAG GATACAATAT GGGAGACATT CCTATCCAAA TCTCAGGGAC AAATTACTAT 
CTGTATCCTT CTGACGGTGG GCAGATTGAA GTGGAAAAGA TCAGGTTTGA ACGAGGCCCA 
ACTTTCATCA AAGTGAAAGT AAAAAAAGAT AAACACAGTT TTATAATTCA CCTGAAAGAT 
CCAAAGGAAA TCGTGAAAAG GGAAAAGGAA GAGGAGCACT CAGAAGAAGA GGAAGAGGAA 
GAGGAGGAGG AAGAGGAAGA GGAAGAGGAA GAGAATGAAC AGAAGGAAGG GAAAGAGGAG 
AATGTTAAAC AAAATGTGGA AATGAAGAGA ACACAGATAA AGAAGGAAGC TGTCAGCAAG 
TTTGGATCCT TTTCTGCCAC CTTAGAAAAT GGAATCTGCC TCTCCATAAG TTACTATGGA 
TCAAATGGAA TGGCGCCAGA GGTTATGGAT TCTGATTTGG AAGCAATGAT GAATATCCCT 
TCAGCTATGA CAGCAACAGT GGTTCCCTCT ATAGTTACTG TTCCCCAAGG CAAAGGCAAA 
ACTAAGCCGA AGGGCAAAGA AAAACACAAA GATTCCTTTA AAGAAGAGGA ACTTCCAAAA 
GAAGAAGAAA AGAAGAATCA CTCACAGGAA GAAGTAGAAC CAGAGATTGT TATACAGGAA 
ACACCTTATG TGCCCACCTT CCAAAACCTA AATGTATCTT GCCCCAATGG GCTCCTGCTT 
ACCTTCATTG GGCAAGAATC TACAGATGAA GAATCTACCT GGAACCTCAT GATCCGCCAG 
AGCTACCCCC AGAGGTTGAA GCACTATGAA TTCTATAAAG CAGTGATGCC ACCCACAGAG 
CAGGAGGCAT CAAGAGTTGT CACCAGCCAG GGCACCGTTA TCAAGTATAT GCTGGATGGA 
TCCACACAGA TTCTTTTTGC AGATGGTGCT GTGAGCAGCA GTCCTGACTC AGGTCCTGTT 
CATGCATCTC CTGAGACGTC TGCAAGCCCC CACAATGGAG ATGTGGCAGA CAACACTTCT 
CAGCATAAAT CCGAAACAGC ACCTTCTGAG ATCATCACCA AGAAAGGAAA AGGTCACAAA 
AATCAAACAT CAACGGCAAG TAAGAGCGAA GCCCATGATA CTCTCCCAGA AGTGGTTCCA 
ACTCCTATGG AGTCCCACAC TGGCACCTGG TTTACAACAA CGCCTGATGG AAGGAGGATC 
GGCACCAAAG GCCTAGAGAA AATCACCAAC TTGAAACCAT TTTTATTCTT CCAGGCTACA 
GATCCCATCA ATGGAACGGT CATGACTACT CGAGAAGACA AGGTGATCAT TGTTGAGAAG 
AAGGACGGGA CCCGGATCGT GGACCATGCC GATGGTACCA GAATCACAAC CTTTTACCAA 
GTGTATGAAG ATCATATTAC TCCCCCAAAT GATGAAGAAA CAACCGAAGA TCCAAGGACT 
GTCACTAGAC AGGTGAAGTG CATGCGGATA GAAAGCTCGC ACTATGCCAC TATTATCACG 
AACTGCGAGG ACAGTAGCTG CTGTGCCACT TTTGGGGACG GAACATCTAT TATTGCAAAG 
CCACAGGGAT CATACCAGGT GCTACCTTCA AACACAGGCT GCCTTTATAT CGACAAAGAC 
TGCTCGGCCA CATATTGCCA TGAATTGAGC AGTAATATGT ACCATCCCTT CCAGAAGCGT 
GAAGAGCTGA GAGCCAGCAG GTACATCATG AAGCACACCT CAGAGGTGAT CTGTGAGGTC 
CAGGATCCCG AGGGGAACAT CTTTAAGGTC ATGGCTGATG GCTCTGTATC AACAGCGTTA 
CCTAAAAAGA AGCTAGAAGA CAAGTTGAAT GTACAGACGG AGGGCTACGA AAGCTTATCA 
TCACTTCACC TTGAAAAGAA TCGCATGCAG ATCTATGGTG AACATGTTCC CAGATTTTTT 
GTTGTCTATA CTGATGGATC AGGAGTGGAA CTTCTCCGAG ACAGTGACAT AGAAGAATAC 
TTATCCTTGG CCTATGGAGA GTCAACGACT GTGGTTCTCC AGGAACCAGT ACAGGAACAC 
CCAGGTGCCC TGAGCATCAC AGTCCTTCGC CCTTTCCATG AGGCATCACA ATGGATCATG 
AAGAAGGAGC TGGACAGCAT CGTTCCTCCA AATCTCCAGT CGAGATCATG GGAGACATTT 
CCTTCAGTTG AGAAAAAGAC TCCAGGACCA CCATTTGGCA CTCAGATTTG GAAAGGCCTC 
TGTATTGGGG CTAAGCAGCT GACAAACATC CCAGCTCCTA TACTTGAAGG CCCCAAGGTG 
CTACAGATGC GTCAGTTCAT CCAGCACGAA GTCATAAAGA ACGAGGTGAA ACTGAAGCTG 
CAAATTTCCC TGAAGGACTA TATAAACCAC ATCCTGAAGA AAGAAGATGA GCAACAAGAG 
ATGACAGTCA AGGATTCCAG AACGGAGGAG GAGAGAGGCA ATGCTGCAGA TCTCCTCAAG 
CTGGTTATGT CTTTCCCTAA AATGGAGGAA ACTACAAAAA GTCACGTGAC TGAAGTTGCT 
GCCCACCTAA CAGATTTATT CAAGCAATCT ATGGCTTCGG CTCCAAAATG TTCACCAGAA 
AACTTCACTA AAGATTTCTT TGAAAAGAAG TGGAGGGAGG CTCCACAAGG AAAGAGTTGG 
AAAGAAAAGC TTGAGCAACA GAGGAACAAT ATTAAGAAAA CACAAAATTA CCTAATGCAA 
ATTAGGACAA AAGAAGTAAC GCCTTATTTT AAGTCTGAAT TGAGCCCATT GATTAAGTCA 
CAGTATGATT ATCTGGAGAA GCTTTCCAAA AACCTGCCTC CGTTTACAAA GAAAAATGAG 
GCTGAAACCA AGACAGCTGA CCCAGATCTT CACTCAGGTA TAGGAATTGG AATAGACTGG 
AAATTTCTGA AGGTGGGGCC CCTCCGTAGG AGGAAATCTT TCAACGTCAG TGTTTCACTA 
GCTACCCTTG CTAACTTAAA GGAAAGGATC CAGAATCCCA AAGTTCAAGA TGAGCTCACT 
GTAGGGAAGT TACTTGAAAA GTATTCTCTA ACAGAACCAG AAAATTTTAT GCCAATCAAA 
ATTCCAACCC AGTCATTGCT GCAGGATGTT ACTGGAAAAG CAAGAAAAGA GAAAGTGAGG 
TTACCTCATT ACATGATGAG CTGCAAGCCC AAGTGTCAGC CTCACACCAA GGTGGAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCC ATTGCATGTG CTGCTATTTA TAACCCAGAC 
GTATCCCCTT TCGGGTTCCA CCTTCTCCCA CCATCAATAC AGTTTGGAGT GCTTAAAGAA 
GGACACACCT ATGCAACGAT TGTGAAGCTC AAGAATGTTG GTGTGGACTT CTGCAGGTTT 
AGAGTGAAGC AGCCCCCACC CAGCACGGGA CTGAAAGTGA CATATAAACC TGGGCCTGTG 
GCTGCTGGCT TGCAAGCAGA GCTAAAGGTA GAGCTATTTG CCATGGCTGT TGGAGAAGAC 
GGCGCCAAGG ACTCAGCACA CATCTCCCAC ACCATTGAAA TCCTGACGGA GCACGATGTT 
CTATTCTTAC CCGTGGAGGC AACAGTTTTA ACAAGCAGTA ACTATGACAA TCGACCAAAA 
GATCTTCCCC AGGGAAGAGA AAATCCCATG GTCTACAGGA CTTCCACAAT CTCCTCCTCT 
GCACTTGGAG TCGTCATGTC TCAGAAGGCC ACTCGTCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449538.1
CDS	140..6901
Translation	MAPKKEKPTGSTSYKIWEPSLIAAHMNQNDWKASIAFVVGNRIEDELPIHALNLAVQLPQRKLFSIVSWKDILQQINEIQALVGATTKKGKKTITGNLPLHYEVLLAAKTIMDNGEKLTLPLVGKLLKFQLLQIKTKDQQRRENEKKMIEERAKSEKDKGKGKSPKEKKAATVKAGKGKSKDQPEATVTVKKTTQLKRRGEDDDTKFYIDDEPDDGAQHYVIVVGFNHPQLLAIMTELGIPITSVIKISSENYEPLKTYLAAVMQQQEALLQPEDIAAENLKRENDIKELEVFWKYLEPILNNEKPETNLLDVARLHYMVKTKHFPSDWSDYEQMLDLGTEIFENVACLMYDSLDWKRQHHHYLQSMQLINVPQVVSDKTMLEAIPIPEPPPSTAPAPGKKKAQYEESHAPPSVAFIITTEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVIATEEDLTPPSLVEPAPRADGLDYRIAAHIVSILPSLCLTEKEKKNLHEIFLTEGESESKTPPKGPLLLNYHDTYAHKKYALKDQKNFDPVQIEEEMQSKLPLWEFLQFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSEVDEAGRLKPTEVISETNVENVNIPWDNPARFAKLIRQRYIHRMSIQKAKPVLVETEHKDRTLFVNQNFAKAEQDAQGDENSPNCDHPAANNVMGSTSNSTRPRYSSDRQLTEKETSGSMCPQPESVEQIMDTEIKGGAATKDDSLEKKPKKMVVEADIEDIKKTQQRSLMDWSFTEHFQPKVLLQVLQEAHQQYRCVDSYYHTQDNSLLLVFHNPMNLHHLHCEYWNIALHSNVGFRNYLELVAKSIEEWVIQEEAKYQEAKMAEELNRAKNELDLKATTAKTSASKIGGPKKSKSNKVSSKTELTDQEKDKEKEKEKEKDKIPFILEGSLKAWKEEQERLAEEERLKEEKKAEKKGKEAGRKKGKDKADKEDTKSLKKKSSSKEKPKEEPSKSLEAIEEAVPLPEPEIVYPFRGYNMGDIPIQISGTNYYLYPSDGGQIEVEKIRFERGPTFIKVKVKKDKHSFIIHLKDPKEIVKREKEEEHSEEEEEEEEEEEEEEEEENEQKEGKEENVKQNVEMKRTQIKKEAVSKFGSFSATLENGICLSISYYGSNGMAPEVMDSDLEAMMNIPSAMTATVVPSIVTVPQGKGKTKPKGKEKHKDSFKEEELPKEEEKKNHSQEEVEPEIVIQETPYVPTFQNLNVSCPNGLLLTFIGQESTDEESTWNLMIRQSYPQRLKHYEFYKAVMPPTEQEASRVVTSQGTVIKYMLDGSTQILFADGAVSSSPDSGPVHASPETSASPHNGDVADNTSQHKSETAPSEIITKKGKGHKNQTSTASKSEAHDTLPEVVPTPMESHTGTWFTTTPDGRRIGTKGLEKITNLKPFLFFQATDPINGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHITPPNDEETTEDPRTVTRQVKCMRIESSHYATIITNCEDSSCCATFGDGTSIIAKPQGSYQVLPSNTGCLYIDKDCSATYCHELSSNMYHPFQKREELRASRYIMKHTSEVICEVQDPEGNIFKVMADGSVSTALPKKKLEDKLNVQTEGYESLSSLHLEKNRMQIYGEHVPRFFVVYTDGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEHPGALSITVLRPFHEASQWIMKKELDSIVPPNLQSRSWETFPSVEKKTPGPPFGTQIWKGLCIGAKQLTNIPAPILEGPKVLQMRQFIQHEVIKNEVKLKLQISLKDYINHILKKEDEQQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSMASAPKCSPENFTKDFFEKKWREAPQGKSWKEKLEQQRNNIKKTQNYLMQIRTKEVTPYFKSELSPLIKSQYDYLEKLSKNLPPFTKKNEAETKTADPDLHSGIGIGIDWKFLKVGPLRRRKSFNVSVSLATLANLKERIQNPKVQDELTVGKLLEKYSLTEPENFMPIKIPTQSLLQDVTGKARKEKVRLPHYMMSCKPKCQPHTKVEDSVGGKVNTSSIACAAIYNPDVSPFGFHLLPPSIQFGVLKEGHTYATIVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFAMAVGEDGAKDSAHISHTIEILTEHDVLFLPVEATVLTSSNYDNRPKDLPQGRENPMVYRTSTISSSALGVVMSQKATRH

Target ORF information:

RefSeq Version	XM_017594049.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 17 (Spag17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594049.1

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA ACCTACCGGG AGCACAAGCT ATAAGATATG GGAACCCTCA 
CTCATTGCTG CACATATGAA CCAGAATGAT TGGAAGGCCT CCATCGCCTT CGTGGTTGGG 
AACCGAATTG AGGATGAGCT TCCCATTCAT GCCCTCAACC TGGCTGTCCA ACTGCCTCAG 
CGGAAACTCT TCAGTATTGT GTCATGGAAA GACATTCTGC AGCAGATCAA TGAAATACAG 
GCCCTTGTTG GAGCTACTAC TAAAAAGGGG AAGAAGACCA TAACTGGCAA CTTACCATTA 
CACTATGAGG TGTTGCTGGC AGCAAAAACA ATCATGGACA ATGGAGAGAA GCTGACCTTA 
CCATTGGTAG GGAAACTCTT GAAATTTCAA CTCCTGCAGA TTAAAACCAA GGACCAGCAG 
AGGCGAGAAA ACGAAAAGAA GATGATAGAA GAAAGAGCAA AGTCGGAGAA GGACAAAGGA 
AAAGGGAAGT CTCCCAAGGA GAAGAAGGCC GCCACTGTTA AGGCTGGGAA GGGAAAGAGC 
AAGGACCAGC CCGAGGCCAC CGTAACAGTG AAGAAGACCA CGCAGTTAAA ACGGAGGGGA 
GAAGATGACG ACACCAAATT CTACATCGAC GATGAGCCGG ACGATGGTGC CCAACATTAC 
GTTATTGTTG TGGGCTTCAA CCATCCTCAG CTATTGGCCA TTATGACTGA ACTTGGCATT 
CCTATTACCA GTGTGATTAA AATATCTTCA GAGAATTATG AACCTCTGAA GACATACCTG 
GCAGCAGTCA TGCAGCAACA GGAAGCTCTC CTTCAGCCGG AAGATATAGC AGCTGAAAAT 
TTGAAGAGAG AAAATGACAT TAAGGAACTT GAGGTCTTCT GGAAGTACTT GGAACCAATC 
CTGAATAATG AGAAGCCTGA AACAAATCTC TTGGATGTTG CTCGACTCCA CTACATGGTC 
AAGACAAAAC ATTTCCCTTC TGACTGGTCA GACTATGAGC AGATGCTTGA CCTAGGCACG 
GAGATCTTTG AAAATGTCGC CTGTTTGATG TACGACAGCC TGGACTGGAA GAGGCAGCAT 
CATCACTATC TGCAAAGCAT GCAGCTCATC AATGTCCCAC AAGTGGTTAG CGATAAGACC 
ATGTTGGAAG CGATCCCGAT TCCAGAGCCT CCACCATCAA CTGCTCCAGC CCCCGGAAAG 
AAGAAAGCAC AGTATGAGGA ATCACATGCT CCCCCATCAG TGGCTTTTAT CATCACAACT 
GAAGTAGACA TGCGGTATTA CAATGACCTG CTGAATCCAA TTCCAGAGGA ATTTATTTCA 
GTGCCCCTGA TACTGCACTG TATGCTGGAA CAGGTTATAG CAACGGAAGA GGATCTCACC 
CCGCCGAGTT TGGTGGAGCC AGCCCCCAGA GCAGATGGGT TAGACTACAG AATCGCAGCT 
CACATCGTGT CCATCCTGCC TTCCCTCTGC CTCACTGAGA AGGAAAAGAA GAATCTCCAC 
GAGATATTCT TAACTGAAGG AGAGAGCGAA AGCAAAACCC CACCCAAAGG CCCTCTCCTG 
CTGAACTACC ACGACACGTA TGCCCACAAG AAGTACGCGC TGAAGGACCA AAAGAATTTT 
GACCCGGTTC AAATTGAGGA GGAGATGCAA TCCAAGCTGC CACTGTGGGA ATTCCTTCAG 
TTCCCTCTGC CGCCTCCGTG GAACAGCACT AAACGGCTTG CCACCATTCA TGAACTGATG 
CATTTCTGTA CCAACGAAGT TCTGAGCTGG AATGAAGTAG AACGAGCCTT CAAGGTATTT 
ACTTTTGAAA GCCTGAAGCT CTCAGAGGTG GATGAGGCAG GACGGCTCAA GCCCACCGAG 
GTGATCAGTG AGACAAATGT TGAAAATGTC AACATTCCTT GGGACAATCC TGCCAGGTTC 
GCTAAGCTGA TAAGACAGCG GTACATCCAC AGGATGAGCA TACAGAAGGC CAAGCCAGTA 
TTAGTAGAAA CCGAACACAA AGACAGAACA CTATTCGTGA ACCAGAATTT TGCCAAAGCT 
GAGCAAGATG CACAAGGAGA TGAAAACTCC CCAAATTGTG ATCATCCTGC TGCTAACAAT 
GTGATGGGGT CTACTTCCAA CTCCACGAGA CCCCGGTATT CTAGTGATAG ACAGCTCACA 
GAGAAAGAGA CCAGTGGGTC CATGTGTCCC CAACCAGAGT CTGTGGAGCA GATCATGGAC 
ACTGAGATCA AAGGTGGAGC AGCCACAAAG GATGATTCTC TTGAAAAGAA ACCCAAGAAG 
ATGGTGGTGG AGGCCGACAT AGAGGACATT AAAAAAACAC AACAGCGCAG TTTAATGGAC 
TGGAGCTTTA CTGAACATTT TCAGCCGAAA GTCCTGCTTC AGGTTCTTCA GGAGGCTCAT 
CAGCAGTACC GATGTGTTGA CTCGTACTAC CACACGCAGG ACAATTCCTT ACTTCTCGTC 
TTTCATAACC CAATGAATCT ACACCATCTG CATTGTGAAT ATTGGAATAT TGCTCTTCAT 
TCCAACGTTG GATTCAGGAA TTACTTAGAA CTTGTTGCAA AATCTATTGA AGAATGGGTC 
ATACAGGAAG AAGCAAAGTA CCAGGAAGCC AAAATGGCTG AGGAGCTCAA CAGAGCCAAG 
AATGAGTTGG ACTTGAAAGC TACTACTGCC AAAACTTCTG CTAGCAAAAT TGGTGGCCCC 
AAAAAATCTA AAAGTAACAA AGTGTCCAGC AAAACAGAGC TAACAGACCA AGAAAAAGAT 
AAAGAGAAAG AGAAAGAAAA AGAGAAAGAC AAGATCCCCT TCATATTAGA AGGCTCTCTC 
AAGGCATGGA AGGAAGAACA AGAGAGGTTG GCTGAGGAGG AACGCCTGAA GGAGGAAAAG 
AAGGCAGAGA AGAAGGGGAA GGAAGCAGGT AGAAAGAAAG GCAAGGATAA GGCAGACAAA 
GAAGACACTA AGAGTTTGAA AAAAAAGTCG TCTTCCAAGG AGAAACCTAA GGAAGAACCA 
TCTAAGTCGC TGGAAGCAAT AGAGGAAGCC GTCCCACTTC CAGAACCGGA AATTGTTTAC 
CCGTTCCGAG GATACAATAT GGGAGACATT CCTATCCAAA TCTCAGGGAC AAATTACTAT 
CTGTATCCTT CTGACGGTGG GCAGATTGAA GTGGAAAAGA TCAGGTTTGA ACGAGGCCCA 
ACTTTCATCA AAGTGAAAGT AAAAAAAGAT AAACACAGTT TTATAATTCA CCTGAAAGAT 
CCAAAGGAAA TCGTGAAAAG GGAAAAGGAA GAGGAGCACT CAGAAGAAGA GGAAGAGGAA 
GAGGAGGAGG AAGAGGAAGA GGAAGAGGAA GAGAATGAAC AGAAGGAAGG GAAAGAGGAG 
AATGTTAAAC AAAATGTGGA AATGAAGAGA ACACAGATAA AGAAGGAAGC TGTCAGCAAG 
TTTGGATCCT TTTCTGCCAC CTTAGAAAAT GGAATCTGCC TCTCCATAAG TTACTATGGA 
TCAAATGGAA TGGCGCCAGA GGTTATGGAT TCTGATTTGG AAGCAATGAT GAATATCCCT 
TCAGCTATGA CAGCAACAGT GGTTCCCTCT ATAGTTACTG TTCCCCAAGG CAAAGGCAAA 
ACTAAGCCGA AGGGCAAAGA AAAACACAAA GATTCCTTTA AAGAAGAGGA ACTTCCAAAA 
GAAGAAGAAA AGAAGAATCA CTCACAGGAA GAAGTAGAAC CAGAGATTGT TATACAGGAA 
ACACCTTATG TGCCCACCTT CCAAAACCTA AATGTATCTT GCCCCAATGG GCTCCTGCTT 
ACCTTCATTG GGCAAGAATC TACAGATGAA GAATCTACCT GGAACCTCAT GATCCGCCAG 
AGCTACCCCC AGAGGTTGAA GCACTATGAA TTCTATAAAG CAGTGATGCC ACCCACAGAG 
CAGGAGGCAT CAAGAGTTGT CACCAGCCAG GGCACCGTTA TCAAGTATAT GCTGGATGGA 
TCCACACAGA TTCTTTTTGC AGATGGTGCT GTGAGCAGCA GTCCTGACTC AGGTCCTGTT 
CATGCATCTC CTGAGACGTC TGCAAGCCCC CACAATGGAG ATGTGGCAGA CAACACTTCT 
CAGCATAAAT CCGAAACAGC ACCTTCTGAG ATCATCACCA AGAAAGGAAA AGGTCACAAA 
AATCAAACAT CAACGGCAAG TAAGAGCGAA GCCCATGATA CTCTCCCAGA AGTGGTTCCA 
ACTCCTATGG AGTCCCACAC TGGCACCTGG TTTACAACAA CGCCTGATGG AAGGAGGATC 
GGCACCAAAG GCCTAGAGAA AATCACCAAC TTGAAACCAT TTTTATTCTT CCAGGCTACA 
GATCCCATCA ATGGAACGGT CATGACTACT CGAGAAGACA AGGTGATCAT TGTTGAGAAG 
AAGGACGGGA CCCGGATCGT GGACCATGCC GATGGTACCA GAATCACAAC CTTTTACCAA 
GTGTATGAAG ATCATATTAC TCCCCCAAAT GATGAAGAAA CAACCGAAGA TCCAAGGACT 
GTCACTAGAC AGGTGAAGTG CATGCGGATA GAAAGCTCGC ACTATGCCAC TATTATCACG 
AACTGCGAGG ACAGTAGCTG CTGTGCCACT TTTGGGGACG GAACATCTAT TATTGCAAAG 
CCACAGGGAT CATACCAGGT GCTACCTTCA AACACAGGCT GCCTTTATAT CGACAAAGAC 
TGCTCGGCCA CATATTGCCA TGAATTGAGC AGTAATATGT ACCATCCCTT CCAGAAGCGT 
GAAGAGCTGA GAGCCAGCAG GTACATCATG AAGCACACCT CAGAGGTGAT CTGTGAGGTC 
CAGGATCCCG AGGGGAACAT CTTTAAGGTC ATGGCTGATG GCTCTGTATC AACAGCGTTA 
CCTAAAAAGA AGCTAGAAGA CAAGTTGAAT GTACAGACGG AGGGCTACGA AAGCTTATCA 
TCACTTCACC TTGAAAAGAA TCGCATGCAG ATCTATGGTG AACATGTTCC CAGATTTTTT 
GTTGTCTATA CTGATGGATC AGGAGTGGAA CTTCTCCGAG ACAGTGACAT AGAAGAATAC 
TTATCCTTGG CCTATGGAGA GTCAACGACT GTGGTTCTCC AGGAACCAGT ACAGGAACAC 
CCAGGTGCCC TGAGCATCAC AGTCCTTCGC CCTTTCCATG AGGCATCACA ATGGATCATG 
AAGAAGGAGC TGGACAGCAT CGTTCCTCCA AATCTCCAGT CGAGATCATG GGAGACATTT 
CCTTCAGTTG AGAAAAAGAC TCCAGGACCA CCATTTGGCA CTCAGATTTG GAAAGGCCTC 
TGTATTGGGG CTAAGCAGCT GACAAACATC CCAGCTCCTA TACTTGAAGG CCCCAAGGTG 
CTACAGATGC GTCAGTTCAT CCAGCACGAA GTCATAAAGA ACGAGGTGAA ACTGAAGCTG 
CAAATTTCCC TGAAGGACTA TATAAACCAC ATCCTGAAGA AAGAAGATGA GCAACAAGAG 
ATGACAGTCA AGGATTCCAG AACGGAGGAG GAGAGAGGCA ATGCTGCAGA TCTCCTCAAG 
CTGGTTATGT CTTTCCCTAA AATGGAGGAA ACTACAAAAA GTCACGTGAC TGAAGTTGCT 
GCCCACCTAA CAGATTTATT CAAGCAATCT ATGGCTTCGG CTCCAAAATG TTCACCAGAA 
AACTTCACTA AAGATTTCTT TGAAAAGAAG TGGAGGGAGG CTCCACAAGG AAAGAGTTGG 
AAAGAAAAGC TTGAGCAACA GAGGAACAAT ATTAAGAAAA CACAAAATTA CCTAATGCAA 
ATTAGGACAA AAGAAGTAAC GCCTTATTTT AAGTCTGAAT TGAGCCCATT GATTAAGTCA 
CAGTATGATT ATCTGGAGAA GCTTTCCAAA AACCTGCCTC CGTTTACAAA GAAAAATGAG 
GCTGAAACCA AGACAGCTGA CCCAGATCTT CACTCAGGTA TAGGAATTGG AATAGACTGG 
AAATTTCTGA AGGTGGGGCC CCTCCGTAGG AGGAAATCTT TCAACGTCAG TGTTTCACTA 
GCTACCCTTG CTAACTTAAA GGAAAGGATC CAGAATCCCA AAGTTCAAGA TGAGCTCACT 
GTAGGGAAGT TACTTGAAAA GTATTCTCTA ACAGAACCAG AAAATTTTAT GCCAATCAAA 
ATTCCAACCC AGTCATTGCT GCAGGATGTT ACTGGAAAAG CAAGAAAAGA GAAAGTGAGG 
TTACCTCATT ACATGATGAG CTGCAAGCCC AAGTGTCAGC CTCACACCAA GGTGGAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCC ATTGCATGTG CTGCTATTTA TAACCCAGAC 
GTATCCCCTT TCGGGTTCCA CCTTCTCCCA CCATCAATAC AGTTTGGAGT GCTTAAAGAA 
GGACACACCT ATGCAACGAT TGTGAAGCTC AAGAATGTTG GTGTGGACTT CTGCAGGTTT 
AGAGTGAAGC AGCCCCCACC CAGCACGGGA CTGAAAGTGA CATATAAACC TGGGCCTGTG 
GCTGCTGGCT TGCAAGCAGA GCTAAAGGTA GAGCTATTTG CCATGGCTGT TGGAGAAGAC 
GGCGCCAAGG ACTCAGCACA CATCTCCCAC ACCATTGAAA TCCTGACGGA GCACGATGTT 
CTATTCTTAC CCGTGGAGGC AACAGTTTTA ACAAGCAGTA ACTATGACAA TCGACCAAAA 
GATCTTCCCC AGGGAAGAGA AAATCCCATG GTCTACAGGA CTTCCACAAT CTCCTCCTCT 
GCACTTGGAG TCGTCATGTC TCAGAAGGCC ACTCGTCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Spag17 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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